LOCUS       DASJPC010000001       147029 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJPC010000001 DASJPC010000000
VERSION     DASJPC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 147029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 147029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..147029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.001"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(110..820)
                     /locus_tag="U4A13_000001"
     CDS             complement(110..820)
                     /locus_tag="U4A13_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN1778339.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(817..2289)
                     /locus_tag="U4A13_000002"
     CDS             complement(817..2289)
                     /locus_tag="U4A13_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1778340.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2427..2900
                     /locus_tag="U4A13_000003"
     CDS             2427..2900
                     /locus_tag="U4A13_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1778341.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(3053..3604)
                     /locus_tag="U4A13_000004"
     CDS             complement(3053..3604)
                     /locus_tag="U4A13_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1778342.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3709..4239
                     /locus_tag="U4A13_000005"
     CDS             3709..4239
                     /locus_tag="U4A13_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN1778343.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4722..5165)
                     /locus_tag="U4A13_000006"
     CDS             complement(4722..5165)
                     /locus_tag="U4A13_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1778344.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5181..6341)
                     /locus_tag="U4A13_000007"
     CDS             complement(5181..6341)
                     /locus_tag="U4A13_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1778345.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6848..8548
                     /locus_tag="U4A13_000008"
     CDS             6848..8548
                     /locus_tag="U4A13_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778346.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8650..9483)
                     /locus_tag="U4A13_000009"
     CDS             complement(8650..9483)
                     /locus_tag="U4A13_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN1778347.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9787..11952)
                     /locus_tag="U4A13_000010"
     CDS             complement(9787..11952)
                     /locus_tag="U4A13_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1778348.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12212..14506
                     /locus_tag="U4A13_000011"
     CDS             12212..14506
                     /locus_tag="U4A13_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1778349.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14499..15164
                     /gene="pgmB"
                     /locus_tag="U4A13_000012"
     CDS             14499..15164
                     /gene="pgmB"
                     /locus_tag="U4A13_000012"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1778350.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            15157..16164
                     /locus_tag="U4A13_000013"
     CDS             15157..16164
                     /locus_tag="U4A13_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1778351.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16186..17205
                     /locus_tag="U4A13_000014"
     CDS             16186..17205
                     /locus_tag="U4A13_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1778352.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17486..18853)
                     /gene="nhaC"
                     /locus_tag="U4A13_000015"
     CDS             complement(17486..18853)
                     /gene="nhaC"
                     /locus_tag="U4A13_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1778353.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18963..20384)
                     /locus_tag="U4A13_000016"
     CDS             complement(18963..20384)
                     /locus_tag="U4A13_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1778354.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20627..22504)
                     /gene="tdc"
                     /locus_tag="U4A13_000017"
     CDS             complement(20627..22504)
                     /gene="tdc"
                     /locus_tag="U4A13_000017"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1778355.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22795..24051)
                     /locus_tag="U4A13_000018"
     CDS             complement(22795..24051)
                     /locus_tag="U4A13_000018"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN1778356.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24163..24368)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24631..25932
                     /locus_tag="U4A13_000019"
     CDS             24631..25932
                     /locus_tag="U4A13_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1778357.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(26001..26600)
                     /locus_tag="U4A13_000020"
     CDS             complement(26001..26600)
                     /locus_tag="U4A13_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1778358.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26854..27735
                     /locus_tag="U4A13_000021"
     CDS             26854..27735
                     /locus_tag="U4A13_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778359.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27748..29358
                     /locus_tag="U4A13_000022"
     CDS             27748..29358
                     /locus_tag="U4A13_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN1778360.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29435..30094)
                     /locus_tag="U4A13_000023"
     CDS             complement(29435..30094)
                     /locus_tag="U4A13_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1778361.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30377..31042)
                     /locus_tag="U4A13_000024"
     CDS             complement(30377..31042)
                     /locus_tag="U4A13_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN1778362.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(31189..31794)
                     /gene="dhaL"
                     /locus_tag="U4A13_000025"
     CDS             complement(31189..31794)
                     /gene="dhaL"
                     /locus_tag="U4A13_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN1778363.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(31822..32832)
                     /gene="dhaK"
                     /locus_tag="U4A13_000026"
     CDS             complement(31822..32832)
                     /gene="dhaK"
                     /locus_tag="U4A13_000026"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN1778364.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(32807..33253)
                     /gene="dhaM"
                     /locus_tag="U4A13_000027"
     CDS             complement(32807..33253)
                     /gene="dhaM"
                     /locus_tag="U4A13_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN1778365.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            33356..33676
                     /locus_tag="U4A13_000028"
     CDS             33356..33676
                     /locus_tag="U4A13_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN1778366.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(33788..34657)
                     /locus_tag="U4A13_000029"
     CDS             complement(33788..34657)
                     /locus_tag="U4A13_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1778367.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(34753..36056)
                     /locus_tag="U4A13_000030"
                     /pseudo
     CDS             complement(34753..36056)
                     /locus_tag="U4A13_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(36053..36562)
                     /locus_tag="U4A13_000031"
     CDS             complement(36053..36562)
                     /locus_tag="U4A13_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778368.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(36737..38872)
                     /locus_tag="U4A13_000032"
     CDS             complement(36737..38872)
                     /locus_tag="U4A13_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1778369.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(38955..40610)
                     /locus_tag="U4A13_000033"
     CDS             complement(38955..40610)
                     /locus_tag="U4A13_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1778370.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(40612..40926)
                     /locus_tag="U4A13_000034"
     CDS             complement(40612..40926)
                     /locus_tag="U4A13_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1778371.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            41096..41356
                     /locus_tag="U4A13_000035"
     CDS             41096..41356
                     /locus_tag="U4A13_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN1778372.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            41370..41684
                     /locus_tag="U4A13_000036"
     CDS             41370..41684
                     /locus_tag="U4A13_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN1778373.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(42414..42968)
                     /locus_tag="U4A13_000037"
     CDS             complement(42414..42968)
                     /locus_tag="U4A13_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN1778374.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(43094..45022)
                     /locus_tag="U4A13_000038"
     CDS             complement(43094..45022)
                     /locus_tag="U4A13_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1778375.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(45328..46044)
                     /locus_tag="U4A13_000039"
     CDS             complement(45328..46044)
                     /locus_tag="U4A13_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1778376.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(46045..47868)
                     /gene="glpO"
                     /locus_tag="U4A13_000040"
     CDS             complement(46045..47868)
                     /gene="glpO"
                     /locus_tag="U4A13_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN1778377.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(47882..49378)
                     /gene="glpK"
                     /locus_tag="U4A13_000041"
     CDS             complement(47882..49378)
                     /gene="glpK"
                     /locus_tag="U4A13_000041"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN1778378.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(49514..50974)
                     /locus_tag="U4A13_000042"
     CDS             complement(49514..50974)
                     /locus_tag="U4A13_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778379.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(50964..52259)
                     /locus_tag="U4A13_000043"
     CDS             complement(50964..52259)
                     /locus_tag="U4A13_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1778380.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(52591..53268)
                     /locus_tag="U4A13_000044"
     CDS             complement(52591..53268)
                     /locus_tag="U4A13_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN1778381.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(53298..54389)
                     /locus_tag="U4A13_000045"
     CDS             complement(53298..54389)
                     /locus_tag="U4A13_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN1778382.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(54490..54924)
                     /locus_tag="U4A13_000046"
     CDS             complement(54490..54924)
                     /locus_tag="U4A13_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1778383.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(55508..56965)
                     /gene="ascB"
                     /locus_tag="U4A13_000047"
     CDS             complement(55508..56965)
                     /gene="ascB"
                     /locus_tag="U4A13_000047"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1778384.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(56962..58857)
                     /locus_tag="U4A13_000048"
     CDS             complement(56962..58857)
                     /locus_tag="U4A13_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1778385.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(58915..60690)
                     /locus_tag="U4A13_000049"
     CDS             complement(58915..60690)
                     /locus_tag="U4A13_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1778386.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(60740..62236)
                     /locus_tag="U4A13_000050"
     CDS             complement(60740..62236)
                     /locus_tag="U4A13_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1778387.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(62430..64052)
                     /locus_tag="U4A13_000051"
     CDS             complement(62430..64052)
                     /locus_tag="U4A13_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1778388.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(64501..65814)
                     /locus_tag="U4A13_000052"
     CDS             complement(64501..65814)
                     /locus_tag="U4A13_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1778389.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(66059..66283)
                     /locus_tag="U4A13_000053"
     CDS             complement(66059..66283)
                     /locus_tag="U4A13_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778390.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(66303..66725)
                     /locus_tag="U4A13_000054"
     CDS             complement(66303..66725)
                     /locus_tag="U4A13_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN1778391.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(66869..67579)
                     /locus_tag="U4A13_000055"
     CDS             complement(66869..67579)
                     /locus_tag="U4A13_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778392.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(67597..68094)
                     /locus_tag="U4A13_000056"
     CDS             complement(67597..68094)
                     /locus_tag="U4A13_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1778393.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(68131..68583)
                     /locus_tag="U4A13_000057"
     CDS             complement(68131..68583)
                     /locus_tag="U4A13_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1778394.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(68718..69323)
                     /locus_tag="U4A13_000058"
     CDS             complement(68718..69323)
                     /locus_tag="U4A13_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1778395.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(69773..70393)
                     /locus_tag="U4A13_000059"
     CDS             complement(69773..70393)
                     /locus_tag="U4A13_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1778396.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(70395..71264)
                     /locus_tag="U4A13_000060"
     CDS             complement(70395..71264)
                     /locus_tag="U4A13_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN1778397.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(71287..71694)
                     /locus_tag="U4A13_000061"
     CDS             complement(71287..71694)
                     /locus_tag="U4A13_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1778398.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(71713..73002)
                     /locus_tag="U4A13_000062"
     CDS             complement(71713..73002)
                     /locus_tag="U4A13_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1778399.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(73029..73331)
                     /locus_tag="U4A13_000063"
     CDS             complement(73029..73331)
                     /locus_tag="U4A13_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1778400.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(73356..75449)
                     /locus_tag="U4A13_000064"
     CDS             complement(73356..75449)
                     /locus_tag="U4A13_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1778401.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(75938..76702)
                     /locus_tag="U4A13_000065"
     CDS             complement(75938..76702)
                     /locus_tag="U4A13_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN1778402.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(76813..77682)
                     /gene="mdcB"
                     /locus_tag="U4A13_000066"
     CDS             complement(76813..77682)
                     /gene="mdcB"
                     /locus_tag="U4A13_000066"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN1778403.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(77707..78783)
                     /locus_tag="U4A13_000067"
     CDS             complement(77707..78783)
                     /locus_tag="U4A13_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN1778404.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(78807..79400)
                     /locus_tag="U4A13_000068"
     CDS             complement(78807..79400)
                     /locus_tag="U4A13_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN1778405.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(79404..81080)
                     /gene="mdcD"
                     /locus_tag="U4A13_000069"
     CDS             complement(79404..81080)
                     /gene="mdcD"
                     /locus_tag="U4A13_000069"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN1778406.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(81070..81369)
                     /locus_tag="U4A13_000070"
     CDS             complement(81070..81369)
                     /locus_tag="U4A13_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN1778407.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(81471..83117)
                     /gene="mdcA"
                     /locus_tag="U4A13_000071"
     CDS             complement(81471..83117)
                     /gene="mdcA"
                     /locus_tag="U4A13_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEN1778408.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(83124..84035)
                     /locus_tag="U4A13_000072"
     CDS             complement(83124..84035)
                     /locus_tag="U4A13_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1778409.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            84155..85012
                     /locus_tag="U4A13_000073"
     CDS             84155..85012
                     /locus_tag="U4A13_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1778410.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(85199..85561)
                     /locus_tag="U4A13_000074"
     CDS             complement(85199..85561)
                     /locus_tag="U4A13_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN1778411.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(85750..85932)
                     /locus_tag="U4A13_000075"
     CDS             complement(85750..85932)
                     /locus_tag="U4A13_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778412.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            85907..87346
                     /locus_tag="U4A13_000076"
     CDS             85907..87346
                     /locus_tag="U4A13_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778413.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(87353..89905)
                     /locus_tag="U4A13_000077"
     CDS             complement(87353..89905)
                     /locus_tag="U4A13_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN1778414.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(90019..91350)
                     /locus_tag="U4A13_000078"
     CDS             complement(90019..91350)
                     /locus_tag="U4A13_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1778415.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(91316..92794)
                     /locus_tag="U4A13_000079"
     CDS             complement(91316..92794)
                     /locus_tag="U4A13_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1778416.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(93099..94934)
                     /gene="tdc"
                     /locus_tag="U4A13_000080"
     CDS             complement(93099..94934)
                     /gene="tdc"
                     /locus_tag="U4A13_000080"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN1778417.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            95404..96831
                     /locus_tag="U4A13_000081"
     CDS             95404..96831
                     /locus_tag="U4A13_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778418.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(96923..97288)
                     /locus_tag="U4A13_000082"
     CDS             complement(96923..97288)
                     /locus_tag="U4A13_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1778419.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(97285..97650)
                     /locus_tag="U4A13_000083"
     CDS             complement(97285..97650)
                     /locus_tag="U4A13_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN1778420.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(97842..98090)
                     /locus_tag="U4A13_000084"
     CDS             complement(97842..98090)
                     /locus_tag="U4A13_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778421.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            98089..98217
                     /locus_tag="U4A13_000085"
     CDS             98089..98217
                     /locus_tag="U4A13_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN1778422.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            98274..99968
                     /locus_tag="U4A13_000086"
     CDS             98274..99968
                     /locus_tag="U4A13_000086"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN1778423.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            99972..100091
                     /locus_tag="U4A13_000087"
                     /pseudo
     CDS             99972..100091
                     /locus_tag="U4A13_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            100619..101752
                     /locus_tag="U4A13_000088"
     CDS             100619..101752
                     /locus_tag="U4A13_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN1778424.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            101783..102103
                     /locus_tag="U4A13_000089"
     CDS             101783..102103
                     /locus_tag="U4A13_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN1778425.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(102132..102596)
                     /locus_tag="U4A13_000090"
     CDS             complement(102132..102596)
                     /locus_tag="U4A13_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1778426.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(102728..103981)
                     /locus_tag="U4A13_000091"
     CDS             complement(102728..103981)
                     /locus_tag="U4A13_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1778427.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(104010..104327)
                     /locus_tag="U4A13_000092"
     CDS             complement(104010..104327)
                     /locus_tag="U4A13_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1778428.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(104353..104673)
                     /locus_tag="U4A13_000093"
     CDS             complement(104353..104673)
                     /locus_tag="U4A13_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1778429.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(105137..106552)
                     /locus_tag="U4A13_000094"
     CDS             complement(105137..106552)
                     /locus_tag="U4A13_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1778430.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(106569..107885)
                     /locus_tag="U4A13_000095"
     CDS             complement(106569..107885)
                     /locus_tag="U4A13_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1778431.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(108035..108733)
                     /locus_tag="U4A13_000096"
     CDS             complement(108035..108733)
                     /locus_tag="U4A13_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN1778432.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(109022..109978)
                     /locus_tag="U4A13_000097"
     CDS             complement(109022..109978)
                     /locus_tag="U4A13_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1778433.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(110091..110924)
                     /gene="msrA"
                     /locus_tag="U4A13_000098"
     CDS             complement(110091..110924)
                     /gene="msrA"
                     /locus_tag="U4A13_000098"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1778434.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(111099..112733)
                     /locus_tag="U4A13_000099"
     CDS             complement(111099..112733)
                     /locus_tag="U4A13_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778435.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(112720..114588)
                     /locus_tag="U4A13_000100"
     CDS             complement(112720..114588)
                     /locus_tag="U4A13_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778436.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            114554..116191
                     /locus_tag="U4A13_000101"
     CDS             114554..116191
                     /locus_tag="U4A13_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778437.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(116336..117076)
                     /gene="truA"
                     /locus_tag="U4A13_000102"
     CDS             complement(116336..117076)
                     /gene="truA"
                     /locus_tag="U4A13_000102"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1778438.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(117267..117902)
                     /locus_tag="U4A13_000103"
     CDS             complement(117267..117902)
                     /locus_tag="U4A13_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1778439.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(118049..119290)
                     /gene="sstT"
                     /locus_tag="U4A13_000104"
     CDS             complement(118049..119290)
                     /gene="sstT"
                     /locus_tag="U4A13_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN1778440.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            119488..120486
                     /locus_tag="U4A13_000105"
     CDS             119488..120486
                     /locus_tag="U4A13_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778441.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            120499..122517
                     /locus_tag="U4A13_000106"
     CDS             120499..122517
                     /locus_tag="U4A13_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN1778442.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(122577..123293)
                     /locus_tag="U4A13_000107"
     CDS             complement(122577..123293)
                     /locus_tag="U4A13_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN1778443.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(123520..124062)
                     /locus_tag="U4A13_000108"
     CDS             complement(123520..124062)
                     /locus_tag="U4A13_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1778444.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(124067..124294)
                     /locus_tag="U4A13_000109"
     CDS             complement(124067..124294)
                     /locus_tag="U4A13_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1778445.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(124373..126253)
                     /locus_tag="U4A13_000110"
     CDS             complement(124373..126253)
                     /locus_tag="U4A13_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1778446.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(126442..127056)
                     /locus_tag="U4A13_000111"
     CDS             complement(126442..127056)
                     /locus_tag="U4A13_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1778447.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            127198..127758
                     /locus_tag="U4A13_000112"
     CDS             127198..127758
                     /locus_tag="U4A13_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1778448.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(128063..128377)
                     /locus_tag="U4A13_000113"
                     /pseudo
     CDS             complement(128063..128377)
                     /locus_tag="U4A13_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(128631..130266)
                     /locus_tag="U4A13_000114"
                     /pseudo
     CDS             complement(128631..130266)
                     /locus_tag="U4A13_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(130821..131384)
                     /locus_tag="U4A13_000115"
     CDS             complement(130821..131384)
                     /locus_tag="U4A13_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1778449.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(131399..132442)
                     /locus_tag="U4A13_000116"
     CDS             complement(131399..132442)
                     /locus_tag="U4A13_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778450.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(132575..132979)
                     /locus_tag="U4A13_000117"
     CDS             complement(132575..132979)
                     /locus_tag="U4A13_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1778451.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(133212..133847)
                     /locus_tag="U4A13_000118"
     CDS             complement(133212..133847)
                     /locus_tag="U4A13_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN1778452.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(134113..135165)
                     /locus_tag="U4A13_000119"
     CDS             complement(134113..135165)
                     /locus_tag="U4A13_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1778453.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(135178..136116)
                     /locus_tag="U4A13_000120"
     CDS             complement(135178..136116)
                     /locus_tag="U4A13_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1778454.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(136567..137217)
                     /locus_tag="U4A13_000121"
     CDS             complement(136567..137217)
                     /locus_tag="U4A13_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778455.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(137317..137826)
                     /locus_tag="U4A13_000122"
     CDS             complement(137317..137826)
                     /locus_tag="U4A13_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEN1778456.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(138027..138896)
                     /locus_tag="U4A13_000123"
     CDS             complement(138027..138896)
                     /locus_tag="U4A13_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1778457.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(138916..140037)
                     /locus_tag="U4A13_000124"
     CDS             complement(138916..140037)
                     /locus_tag="U4A13_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1778458.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(140039..140434)
                     /locus_tag="U4A13_000125"
     CDS             complement(140039..140434)
                     /locus_tag="U4A13_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1778459.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(140453..141259)
                     /locus_tag="U4A13_000126"
     CDS             complement(140453..141259)
                     /locus_tag="U4A13_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1778460.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(141252..142040)
                     /locus_tag="U4A13_000127"
     CDS             complement(141252..142040)
                     /locus_tag="U4A13_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1778461.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(142054..142530)
                     /locus_tag="U4A13_000128"
     CDS             complement(142054..142530)
                     /locus_tag="U4A13_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1778462.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(142602..145259)
                     /locus_tag="U4A13_000129"
     CDS             complement(142602..145259)
                     /locus_tag="U4A13_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1778463.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(145278..145682)
                     /locus_tag="U4A13_000130"
     CDS             complement(145278..145682)
                     /locus_tag="U4A13_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEN1778464.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(145914..146171)
                     /locus_tag="U4A13_000131"
     CDS             complement(145914..146171)
                     /locus_tag="U4A13_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEN1778465.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(146296..146628)
                     /locus_tag="U4A13_000132"
     CDS             complement(146296..146628)
                     /locus_tag="U4A13_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778466.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        43987 a        30308 c        24605 g        48129 t
ORIGIN      
        1 gcactaagcc caatcagatg aaaaatatgg agtgctattt ttatctgatt gttcttactg
       61 cttgatgcag agcagctttt tcacaacctc ctacatggtg ttcaaaatat tattcgcctt
      121 ttgcttcttt cactaatttt gcgaatgctt gttgttggaa ttcagcagct aaggctttcg
      181 tacgttgttc ttgctctgct ttgctcattt ttggattttc agtcattgct tttaccatac
      241 cgtcttgaac ataagctttt gcttcacttt ccatcgtact ttgctgtttc ttcactaatt
      301 cagctaactt ggctgcttgg tccgtactta attctaccca tgtctctggg attttgaatg
      361 tattcactcg gctgtcatat tcgtgattgt tatcagcaat attgaaccag aatttataca
      421 tgtcgttttt gtattcccaa taagtagttt tcgtcacttt ttcagcatcg ccgtctgtct
      481 tttctacttt atttgtttcg ttatccgtac cagttgcttt tggtttttct tgtttttcat
      541 ttgttcggta aagatacact ttttcttttc cattccctaa agctttatac aaaagcatat
      601 ctgctccttg ggaatcagca ctggaaacaa ttttttctgt ttttgtttcc gttacttttt
      661 ccattccaaa gtgattggac aaattagcga cgatcaaact tagtgaagcg acgaagatta
      721 gtccgaaaat caaagataaa aaggtttgcc atgttttttt agcgaacacg ttcgttaaag
      781 caaaagcaag caagctgaca atcaaaactc caataatcat gcattatccc ccgctttcat
      841 tagaacaggt tcttttttct tcaataaaaa ggcaataata aagccaagaa tcccaaagat
      901 gatcgctacg ataaacgcgg catggtaacc tgacagtgtg gcattgattg cctgatcttt
      961 ataagctaat ggcgtattat ccaatacaga tttgcttggg atatttgctt ttgtcacatt
     1021 cgtcaacaca ctgatcaaaa tagctgtacc aatcgagctt gcgatttgac ggaatgtatt
     1081 atttaccgct gtcccatgac tgatcaagtt atttggcaat gcattcatac cagaagtcgt
     1141 taccggcatc atgaccatgg agatcccaaa catacggatc gcataaagga tcacgatata
     1201 taaaactgga gtcgtttttg tcaagaaagc aaatggtgca gttgccgctg tcaaaatgaa
     1261 catccctgtg atggctaaac gtttcgcgcc gtatttatcg aaaatcgcac ccgtcacagg
     1321 catcatagcc cccatgatca atgcacccgg caacaacatc aagccagaat ggaatgctga
     1381 ttctccacga atattttgaa tatacaatgg tacgaccatt tctgccccta ccatcgccat
     1441 atttgttaca ccagctaaaa cagctgcaat cgtgaatgta gatgatttga atacacgaag
     1501 ttccaagaat ggatgttcca aatgtaattg acgccatgag aacaaggcga tgaaaatcac
     1561 accaacaact aagaaaccaa ttactttcaa gcttccccag ccgtcatctc ctacactaga
     1621 aaatccgtaa agtaaactac cgaatccaat cgttgataaa atagcggata aaatatcaat
     1681 gctaggattt gacaatggaa taacactttt cattaggaag aatgctaaaa ccaagacaag
     1741 aacaacgatt ggcagtacca taccaaacaa gtcacgccaa tggtagttat caatgatata
     1801 accagataat gtagggccta aagcaggagc aagtccaata acgatccctg acatccccat
     1861 tgctgatcct cttttttctg gcgggaaaat agatagcatg atattttgca ataatggcat
     1921 cgatactccc acgccgcaag cttggatcaa gcgaccgatt agtaatgtac taaaattagg
     1981 tgctgtaaag caagtaatcg ttccgatcaa aaagatcgtc atggcagaaa gatataaatt
     2041 tcttgaactg aatttattga tcagccatgc tgttattggg atcatgatcc cgttgactag
     2101 caagaaacca gtcgttaacc attgcacatc agacgctgaa atatcgaaag ctttcatcaa
     2161 tgtaggaaaa gctgtcgtca ataaggtctg attcaaaacg gtacaaaatg taccaattaa
     2221 taagacaaca actaaaagtg tccgattata tggttttccg taaatatcta cgggttggtt
     2281 gttactcata tttctccttc tttctgaatt ttttctgaaa ctactagaat actttagtcc
     2341 taaattcgct atttgtcaac ctacttgaca cataagttaa tacggatata attaagggaa
     2401 gcaagaggac aaaaggagtg acaaaaatgg ttggggcttc ccttaaacgt ttgttggaaa
     2461 atgatcaatt ggttgttggc atcagtgaat taagtgatat cgtaggtgtg tctccacgtc
     2521 aattaaggta ttgggaacaa aagggattca ttcgttcgat cgaagctgat gcgaattgtc
     2581 ctagaaaata tcgtttacct acagtcataa aagtagaact gatcaaaaaa tatttagatg
     2641 acggatttac tttgtcaaaa gctgcagaaa aggcagaaat ccgccaaaag aaaatgcacc
     2701 atgtccgtaa agttttttca aaagcaatca aagatatcga attgatcgac gaacgctata
     2761 cgattttaca aatcggtaac ttcaaagaag agaatgctaa gatgtacatc attcatgaca
     2821 acgagacaga cgaattaact tacaagctcc ttcctgtcga tgaaaaagct gactacgaac
     2881 agttgttaaa agaactctaa acgccactaa tctcctaagt accaagcttt ttaataagta
     2941 gatgctacat aaatacttga aaaaaggcct gtttgaacat aagaatatcc gaacggtgat
     3001 ctgcgtttct ttcatataga aaggaattct ccatcactgt tcggatattt ttttattttt
     3061 ttcggttact ttcttctaat acatctgtta gccaatttgc atattctgtt tcccgattga
     3121 tcgcatgcgc taaaatcagg tagtgtccgt aattttcatg tttttctttt tcagtaggaa
     3181 agatcgtttt ttttcgctct aatagatgaa gtcgtttttc ttcatgaagg tcacgctgct
     3241 gctgaataat ttctactaat gccggatcat tcacgtcttt gacgaaatac aatttcagaa
     3301 caaattcgtc tcgattaacc ggtagttcat tggttggtgt atgaatccac tctgccaatt
     3361 gttccttccc ttcttctgtt aattcataaa ctttcttttc caatttttca cctgtgatct
     3421 ctacttgatg ctgaatgatt ccttgctctt ccatttttgc aagttcagga taaatctgac
     3481 tatgttttgc ttgccaaaat tctcctattt catttttgaa cgattgattc aattcatatc
     3541 ctgtcatctt cttcttactt aataacccta acaaaatata tcgaagtgta ttttttcttg
     3601 ccatcagttt tccctccttg acaaatcttt aaatccattc tatcataaat gagtaatcga
     3661 aaacatgtag tttattacat gttttaattt ataaggagat gcttaaacat gaaagaattt
     3721 aaagaattgg aagattttat tggaactcac tttatctata cgtatgacaa tggttgggaa
     3781 tatgaatggt atgccaaaaa tgaccatact gttgattacc gtatccatgg cggaatggtc
     3841 aaaggccgtt gggtaaaaga ccaagaagct gatatcgtta aattgacgga cggcgtattc
     3901 aaaatcactt ggacagagcc aacagggaca gacgtggcat tggactttat gccaaatgaa
     3961 aagaaacttc acggaatgat ctttttccct aaatgggttg aagaacatcc agaaatcact
     4021 gttactttcc aaaacgaaca tatcgcagaa atggaagcag ctcgtgaaaa atatgaaact
     4081 tatccaaaat tacttgttcc tgaatttgct acgatcactt atatgggtga tgcaggtata
     4141 gacaatgaag atgttattag cgaagaacca tacgatggaa tgccagatga tatccgtagc
     4201 ggaaaatact tcgatgaaaa ttataaacga gtaaaataag gttgtgaaag agaggttgtg
     4261 aaatcagcgt tctgacctga gtattagaga aagaaacgga aaaatagttc ctcatgtttt
     4321 gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatc aagaggttgt
     4381 gaaatcagcg ttatgctttg agtaataaga acgctagtta gaaaatagct tttcatattt
     4441 tctaggctag tgggcttatt accgagaagc agccgtttga acaccgttta tcaagaggtt
     4501 gtgaaatcag cgttttgacc tgagtattag agaaacaaat ggaaaaatag ttcctcatat
     4561 tttgccgttt tttgagctaa taccgagggt cagctgattg aacaccgttt atcaaagatt
     4621 gtaacaactg catattcaca cagttaaatt agtgacttca aaatgaaata atgctcccag
     4681 caaaaaatta ggctgggagc attatttcgt tattctgttc atcaaaaatc agcgttttga
     4741 ttccatttca tcacaagttc ctcctcattt actgcttcat ctttttgctg tgtttgaatt
     4801 gtaaaacctt gtgaaagata gaaatgaata gctgattgat ttttttgata gactgtcaaa
     4861 gtcagtgttt tattggtttc ttttattttt ttcattagtt gcgtaccgat tctttgttgc
     4921 cggtattccc ttttcacaaa gattcctgcg ataaactgat caatcacccc agcaaaacca
     4981 ataatttctt gatctatttc tgctaagtaa atcactgcat ctggtaaagc tttcctaaca
     5041 gattctactt gattctccca ataagttgat ggaatgaatt catgagaatc caagttagca
     5101 tgcagccaaa tcgatagaac agattccagt tctttggcag acaattttgt tgttgtgcgg
     5161 atcatttttt ctctccttac ttattcattg attttaaaat aaaaaccatt tttttgaagc
     5221 tcttttacaa gttttcgccc ctcaaaaaag tatatcttct cttgttttac ttttctaata
     5281 ccaaaagaaa cagcaacttt aggatcatca gctgaaacga ttcgataagt tccaccaggc
     5341 aattgatcca gcttgacttt cccatgatgg tcgctttgta ctgaaaacgg cacaccattt
     5401 ctagtaagtg tttttttctt atgtttcaaa atgaatgaac taccatttac cggtttccct
     5461 gacgaatcac taataataaa tgaaaaatca agcacttttt tacttaatct atactgatag
     5521 attagtcgtc cgataaacac taacagtatg actacagcta ttatcgttcc tgcaagaata
     5581 aaccattgcc gcatggttct ttcctgattt ccttttcctt cttctttttt taccgattcg
     5641 ttataaggaa tccgatgtcc tgtcactagc aaacgatgcg tgttgatcat atacggcgta
     5701 catgtaagca aggtaactaa gtcttctcct tctgaaatcg ttagaacaga cgtgtcatct
     5761 ggcagtacca cttgtatatt tttaacttga taagccaatt tctcccccaa aacagtcaaa
     5821 ataaacgtgt ctccgttttc taaacgatct aattcggtaa ataaccgtcg attaggtaaa
     5881 ccgctgtgag cagagatcac tgaatgcgtc gaatttccac caagcgggaa agaggttcct
     5941 tgcaagacgc ctgctccatt ttcaaggatc ctaccattga gccgatcata aagtgaaata
     6001 tttaattgga tcttaggtat attgatactt ccgatcagcc attcctctaa ttggctggat
     6061 gttgtacttt catttctgtt cttttcattg aaaggatcag taccaggatt gaatccttgg
     6121 tcagttaatc gttgtttttt ctcctccatt ttctttcgct gcacttcaat ttcagctgcg
     6181 gtccgttttt gtacttgttc gatacgtttt tgatcaataa aactatttaa agaatccaca
     6241 taaaatggat ataaagcgat cagtaaccct acgacgatac atattaataa aaacaaacga
     6301 aacaaaagtc gacgagactg tttttgctta ctctgcttca catgtgtcct cccttgcaag
     6361 tttttttgtt ctttctttta ttatatgggt tcaggaaaaa aatagaaaat gaaagccgat
     6421 gaggttgtga aaaagctgtc ctgcatcaag taataagtac agcccaaaaa aatagctact
     6481 catatttttt gttgggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag
     6541 gaagaggttg tgaaaactgc gttatgcttt gagtaataag agcgctagtc agaaaacagc
     6601 ttctcatatt ttctaggcta gtgagcttat taccgagaag cagctgattg aacaccgttt
     6661 accgagaggt catgaaaaag ctactttgtt tcgagtatta agaaaaattc tgtttcaaaa
     6721 caaggagatg caacaaaact tatgtcgcat cctctctcct tagaactttc acttatgttt
     6781 ttcttgtaaa attcataaat attttcatga attttctaat ttcttgccta cctctcgcat
     6841 aggatatatg atgaatttat ctaaagtttg caaaggagtt cgacaattga aaaaatatgg
     6901 cccattttta gcaatttttt tcattgcttt ggcatttctc atgcctcatt tattcacaca
     6961 taaactaatt ttgggtgctg atgccccatt ccattacaat cggttctatg atgccgcgca
     7021 acaaattaaa tatggggatt tccattattt tccttccctt tatggattcc agcaaagtgg
     7081 caggatcgtg aatgccgttt acggcccatt tttcgcttat ttacaaggat tgatcgtgtt
     7141 gcttgcaggt agctggtttc attatcagat tctttcaaat ttcattcttt atcttatttc
     7201 aggattttct atgctgattt tactgagata tgtcaatgtt cgaaattggg taagtgtacc
     7261 agtagccgtt ttattcatga gtacgtattc tattcaatac tggtctatca accaaggatt
     7321 cactagctgg gggacttgtt tttatccttt atgtatgatc cctattgctg atttcgtcct
     7381 tcataaaaag ttccctgtta tcaaagtcgc tgtctcagtg ggacttatga cacagatcca
     7441 tttactgtct tcgatcatgt tagctttgat gtatatccct ttttatcttt attacttttt
     7501 taaccagact gaaaaaggaa aagcactgct tcaactgatt aagtcggtct tgctttattt
     7561 tgttctgaca gcaaatattt gggcaggatt agctattgtt tacagtggga ataagattct
     7621 tcctcccttt gttaacagag aaatgagtca aaaggcgata gatctggatg gcagttattg
     7681 gctccattac cctaaggcct ttcctatatt gatattatca ggttttttgc tttttatttt
     7741 ctttcaacaa aagaacacct tattccaaaa aatcgtgttg tacacaagtc ttgttttctt
     7801 ttttctttct actagcttga ttccatggac gacattgatc gaaaaaaatg tgccttttat
     7861 tagtttgatc cagtttccat ttcgattttt cgtgccattt acgctattgc ttctacttta
     7921 ccttgccctt tcactgaacc agtggtctga acaaaagtgg ttccgtcttt taagtatatt
     7981 tggcattctt attttttcag cacagacgat ccagaactta taccagcatc tggaaaagtg
     8041 ggaaaatgaa acatttgtca gtcgacatac ttatctattc gatacgccgg aagaagcaag
     8101 gaaaacattc ttcagcaaag atatgtctac cagcttgttc gttttccaaa aaaccacacc
     8161 tgactatctg ccaatctata aggagacaaa agacaataaa tacgaccggt ataacgaaga
     8221 aatactagaa aacgaaacaa cttttataaa aacacatcca ggcggtcttt tgacaatcaa
     8281 gtggaaaaac actggggaca aaactgttca tatccctgtt atcgtctacg atcggactgt
     8341 gctacagcaa aatggaaaaa cattgactga ttatgaagta acagatatcg ggaccccaat
     8401 cgtgaagcag aaaaaaggaa tcaacgagct cacgcttcat tatcaaacgc caatctattt
     8461 ctatttcatt ctttctctta ctttgattgg ctggtttacc ttgctatgtt tatttattta
     8521 tcatagatac aagcttttac gtgcttaggc aaaagggata aatacatccg atcaaaaaac
     8581 tctccaaacg gtataatcgt ttggagagtt ttattttaat cagttttttt atgttatatg
     8641 ttatattttt tattcgttca catctaattc gatttccacc ccgtagtgat cactgatgat
     8701 tggactatcg atcccatcaa aaatccgttg atatttttta acctgcattc cttcagggac
     8761 gaaaataaaa tctatacgca atttttctgt attttgttcc caaccatcta ttttcttttc
     8821 cacagtagct gcttttcctt tttcattcgc gacaacatac gaatcttgaa gaagaaggca
     8881 actttctctt acagtataat aaccttgaga gtctactgga ttattgaaat cacctaggaa
     8941 aaaaagtggt acgcgagttt ccaaaaagta gttttccaag tttttccact caaaataaaa
     9001 gccacttttt ttatctgtcc accaagaaaa gtggcagctc gagacaaaaa tgtcctgatc
     9061 atctatcttt gttttcccaa tcaagatctt gcgtgtctga taatcttctt tcttttgaga
     9121 ttgggagacg gtaaggacct gactttccaa tgtttttttc gtaagcagtg catttccttc
     9181 ttcatattta tcataaccga tgtggctcat ttcccaactc caaaaatact cttttccgtg
     9241 ttcctttaaa agctgaacta aacggtaggc aaaattatct ttacggatag gtacttgatt
     9301 ctttacctga cagaacttca ttagcttcgc aggttctact tcttcagatt ccagtaattg
     9361 gttgatttct tgtaaagcaa tcacttcata atcttctaac aggatcttat ctgctaattc
     9421 ttgcaatttt ttctctggtt ctggctcaag ccagctatgc gtgttgagtg tcatgaattt
     9481 cattggtttt ctccttgtct tcatttcgaa acaaaagggt tgtgacaaaa tttttgtcac
     9541 aacctctcca gcaaatagtg ttctcctgct aactcctcag taataagctc aatggaccag
     9601 gagctatttt ttgtttggct gtttttatca cttggtgcag ggcagctttt tcacgacctc
     9661 tccactaaac agtattcaaa ctgctgcttc tcggtaataa gcccaaccaa acgaaaaata
     9721 ggaggagcta tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac
     9781 ctctccttac attttaatgg agccgatcac tttaccagct gtcactgtac ctaattgctt
     9841 gatcactact tcttctactt tatcgtttgt aaatacgatc atgatcgttg gatctttttc
     9901 ttcgttttgg atcacatcaa gatccatctc agctaatttc gttcctgttc ctacttcttg
     9961 gccttctttg acagcaatct caaaagctgg ctgattcatt tggactgtat cgattcccat
    10021 atggatcaaa acttcgatac catctttcgt ttccagccca attgcatgtt tgctagggaa
    10081 aatacttgtc acttttccag cgatcggcgc aacaacttct cttgatgttg gaataacagc
    10141 aaagccgtca cccatcattt tttgtgagaa taccggatct tttacttcag ataaaggaat
    10201 cacttctcca gaagctgcgg aaactagttc ttgttctata cctaagtatt gcttactacc
    10261 tgcttgtcca tttcctgtag aaaccatttc tggctctgga atctctgctc ctgattgcag
    10321 cagatcttcg atatctgatt tcaagacatc tgcttttggt ccataaactg cttggactcc
    10381 attgtctttg acgatcaatc ccatcgcccc tgctcgtttc caggcttctt cagtccctac
    10441 tttttctcga tcatttacac ttacgcgtaa ccgagtcata caagcatcga catctttgat
    10501 attttctttt cctcccagca aatgaatgat ctggaccact tgctgatctg ctgttcctgt
    10561 tccgctgtta gatacaacgg tttcttcact gtcattgtcg taatttccgt tacgtcccgg
    10621 tgtagcaaag ttgaattttt tgatcaagaa atttgctaag aaatacgtaa caacaccaaa
    10681 agcgatcaca cacaacacga agttgataag atctccacct agacccgctt tgatagctaa
    10741 cggtgtccga gtcagcaatt cgatattccc aaatgaatgg acccgcaatg gcaagatatc
    10801 tgccatcgca aatgcagccc cttggatcac tgaataaatg acatataatg gcacagcagc
    10861 aaacataaac atgaactcca atggttctgt tacacctgtt agaaagacag ccaacgctgc
    10921 tgaaaaatac attgatttat atcttgcttt cttatcagga tcgacattac gatacatcgc
    10981 aaaagccatt cccatcaaga tacctgaaga accaatcatt tgtcctactt tgaaacgagc
    11041 cggcgtccag ttatgcaaca caaactcata ttgcgacata tcccctgcac ctttaagatt
    11101 cactaaatca gtggcccaag ccaaccataa cggatcttgc ccaaatactt gtgtccccgc
    11161 ctgtgcgcct gataaaatct catacgatcc gccaagctgt gtatagttga taggaatcgt
    11221 cagcatatga tgcaatccaa atggcaatag taaacgttct aatgttccaa ataaaaacgg
    11281 tgctagaatc ggtgcgcttt cttgtgattc tgcaatccat aaaccaaaat tattgattcc
    11341 agcctgtacg tttggccaca ccacagcaag aatcaatgcc acgattgttg accataagat
    11401 aacaacaaac ggaacaaagc gctttccatt gaaaaatgac aaagcatccg gcaattttct
    11461 ataattatag tacttattat aagccattgc cccgacaaat ccagcgatga tccctacgaa
    11521 tactcccata ttcaaagctg gtgcttccaa tacacttgta aaaaatcctt taaccatgat
    11581 cttcgtaccg aacagagtgt gggtaaatgc atcttcattc gcaagcatgt cacttgtgac
    11641 accaaagata gccccagtga tccgattgat taaaataaat gagatcccag ctgcaaatgc
    11701 tccgcctgct ctttcttttg cccaacttcc tcctattgct aaagcaaata aaatatgaag
    11761 attaccaata attgcccaac caatattttc aattactccg cccgttgtga caagcggcgt
    11821 ccaatcagga ttgatcaagg gaattgattt ccctatactg atcatcaacc cagctgccgg
    11881 cataactgct accacaacca ttaaagcctt tccaaatttt tgccaaaact caaaactaaa
    11941 tatctttttc attccgttta gctcctttac gcaatcggtt tcgttaaacg tattatagcg
    12001 tgcgttttct tttttgtaaa tgctttattt tttatttttc tgtctatacc attctataaa
    12061 acctataata cgtgtgttcc cttctataat tttctcctaa aaatttattt ttttaagctt
    12121 ttttctatga aatattttct tttgtctatt gacttgtgca atcggttgcg tttattatga
    12181 acgtataaaa cagctcatta ggaggagaaa aatgaaacag atcaaacgac tatttgctat
    12241 tgatccatgg aaaatcagca cgacacaatt agataaaaaa aatcttcgac tgcaagaatc
    12301 tttaacaagt attggaaacg gttatatggg gatgcgcggg aactttgaag aaacttactc
    12361 aggggaccac catcaaggta cttatttggc gggtgtctgg tatcccgata agacacgtgt
    12421 cggctggtgg aaaaatggtt atccagaata ctttggaaaa gtgatcaacg ccttgaactt
    12481 tatcgcgata gatatttcga tcaacgatca gctgatcgac ttggcttctt tggaaccaga
    12541 agacttttac tgggaattag atatgaaaaa tggtgtatta tctcgtacat tcacaatcac
    12601 tacttctact aataaagtac gcatttcctt cgaacgtttt ctgagcattg tcaaaaaaga
    12661 atcagcttat atctatttaa ctatagaaat gcttgaaggt agcggaacag tcaaactcgt
    12721 atcaaaattg gatggaaatg ttcaaaacga agacagcaac tatgatgaac atttttggaa
    12781 tgagattgat cgtggaacta tagaagaaat cggctatttg acgactaaaa ctattccaaa
    12841 caactttggg attgaacaat ttactgttac tgcagcaatg cgccatttcg ttaatggaaa
    12901 atcagtcatc cctgactatc acgaagatac tttagcatta catggttgct tgtctgcaga
    12961 attatctcaa ggtgagacat ggtcagtgga aaaagaaatt atcgttctta ctagtagaga
    13021 tattccagaa gcgcagcaag cagaaaaagc tgtctcagaa ttaacgaacc agacaaacaa
    13081 ttatatccaa gcaaaagaag agcaaacagc ggcttgggca gaacgatgga agcttgccga
    13141 tgtggtcatc gaaggagacg aagaagcaca acaaggtatt cgattcaatt tgttccaact
    13201 attctctact tactacggcg aagatgaacg attgaatatc ggccccaaag gattcacagg
    13261 agaaaaatac ggcggagcca cttactggga tacagaagcg tatgctgtcc ctctttatct
    13321 tgctttagcc aaacctgaag taactaagaa tctgctgaaa tatcggcata atcaactgcc
    13381 gcaagcaatc cacaacgctc agcaacaagg attaaaaggc gcgctttatc ctatggtcac
    13441 cttcactggt gtcgaatgcc ataacgagtg ggaaatcaca ttcgaggaga ttcaccgaaa
    13501 tggcgcgatt gcctatgcaa tctacaatta tacgaattac accggcgata cggaatattt
    13561 gaaaaatgaa ggactggaag ttttagcaga aatcgcacgc ttctgggcag accgtgtcca
    13621 tttttcaaaa cgagcaaata aatacatgat ccacggtgtg accggcccaa acgaatatga
    13681 gaacaatatc aataacaatt ggtatacaaa tacgatcgca gcttgggtct tgcgttacac
    13741 gcgagaaaat tatttaactt atcaaaaaga agctgctatc actatcagcg aagaagaact
    13801 agccaaatgg aaagatatcg cagagaacat gtacttccca gttgacgaag aattaggaat
    13861 ctttgtccag catgacacat tcttagataa agatctcatg cctgtatccg atattcccac
    13921 aagcgatctt cctctgaatc agcattggtc ttgggacaaa atccttagat cttgcttcat
    13981 caagcaggca gatgtactac aggggattta tttcttcaat gatactttta caaaagaaga
    14041 aaagcaaaaa aactttgatt tctatgaacc aatgactgtc catgagtctt ccctttcgcc
    14101 aagtatccat tcgattttag cagcagaatt aggaatggaa agcaaagctg tagaaatgta
    14161 ccagcgaaca gctcgcttgg atttggataa ttacaataac gacacagaag acggcttgca
    14221 catcacttcc atgactggta gctggttgtc gattgtccaa ggatttgctc aaatgaaaac
    14281 tgcccatgat cagttgagat tcgcaccatt tctgccaaaa acttggaaag cttattcttt
    14341 ccatatcaat tatcgcggac gtttattgtt catctcagtc acagatgaac aagttacttt
    14401 cgagttatta gaaggttttc ctctagaaat ttacgtctat gaacagtctt attctttagc
    14461 ggatcgtcta gaaataacgc taagaaagga ggaagcaaat gtttaaaggg gtattgtttg
    14521 atttagacgg tgtcattacc gatactgctg aattccatta tcgtgcttgg cgaaagcttg
    14581 gtcaggaaat cggcattacg atcgaccaca cattcaacga acagctaaaa ggcgtcagcc
    14641 gagaagactc actctctttg cttttagctt atggcggaaa agaacactct ttttcaaagg
    14701 aagaatttgc agaattagcg aaaagaaaaa acgattatta tttagagatg atccagacga
    14761 tcgaaccaaa agatgtcttc cccggggtcg tgcctttact ggattcttta aaagaagaaa
    14821 aaatcaagat tgctcttgct tctgcaagta aaaacgggcc attcctactt gaaaaaatgg
    14881 gtcttacacc ttactttgat gctattgccg acccggcaga agcaacaaac ggaaaacctg
    14941 cgccagatat cttcctcctt gcagcaaaag ctgtcggact aaaagcagaa gaatgtatcg
    15001 ggatcgaaga cgcgcaagcc ggtatccaag ccatcctctc aagtggcgct caaccaattg
    15061 gcgtgggacg tgctgaagac cttggagagg agatcccgct tgtccctgat acgacttttc
    15121 ttacgatcga ttacttgaaa gaaaagtgtc atgaccatgg ataatccaga gatctccgtc
    15181 gtaccagttc cagagactga aataaagaaa atcacattga agaacgacta cttagaagtc
    15241 gttcttctca attacggtgc ccgcctgcac caaatcttcg caccagacaa agaaggaaaa
    15301 tcagaaaatg ttttattgtc ttacgatcgt ttcgaagatg ttctaacaga caaaagcttt
    15361 tttggagcaa ctgtcggacc tgtagccgga aggatccggg atgcttcttg gggtgagcat
    15421 agattggaga aaaactgcgg atcacaccat atccacggtg gcacgaaagg ctggtctttc
    15481 caattttggg aagtagaagt tttcaaaact ccatcatcaa taggtgtggt cttttactta
    15541 aaagatgaat tttctaccta tccaggacca atcactgcta cgatcactta ccgtttaaca
    15601 aaaaacgaat tagagatgac aacctctgct agtagccttg ttgaaaccat ctgcaatccg
    15661 accaaccatg cctacttcaa cctttcagga aatggaacaa gagacatcta tgaacatcag
    15721 ttaacggtct ttttagatgg tgtgcttgaa ttagatcaag aaaaactgcc tactggaaat
    15781 tggacaaaaa aagaaaaact gccaatcgac tttagaaaat ccccaacact gcaagagatc
    15841 cttgcatgtt atccagatgg actagatgat gtatttcttt tgcatcatcc taggctttcc
    15901 aaaacttcct tacaattatt cgagaagcat tctggtcgcc aaatgactat agcaacttct
    15961 aataaaagta tggtattatt ttctacgact ggattcgaag ctgatttctc tgtcaatgga
    16021 aaacagatgc attcaaacta cggattagca atcgaaccgc aagaaatccc tgatatcgtt
    16081 catttcccca aatttggatc gatcaacctc catcctggtc aagaacggat cagtcagacg
    16141 atttatcggt tctctgcaca ataacaagag aaaggtgttt ttatcatggc tattaccgta
    16201 aaagatgtgg cgaaaaaagc cggtgttgcc acctcgaccg tctctcgtgt catcaacgat
    16261 catcctagta tttccgagac aacaaagaag aaagtccgaa aaattatgga tgaactaggt
    16321 tatgttccta acatgactgc ccgcaatcta ggaaaacgca tttccagtgc catcggcgta
    16381 atcttacccc ctcttgattc aaaagagcgg atcggtaacc cattttatct tgagatcatg
    16441 gaagcaatca acgaagaagc gcggaattat gatatgacta cagcaatcgc tactgctaaa
    16501 aattttgaat tgctgttaga gaacgtccag cgtatgcatt tacaaaaaca agtagatggt
    16561 tttattctag tgtattcaga tagtcaagat cctgtcattg attatttata ccaaaaccac
    16621 atccctttca ctttgatcgg acagccttat catcatgaga acgaaatcgt ttatgtagat
    16681 aatgataacc agcttctagg taaacaagca acagaatttt tgatccagaa tggacataag
    16741 aatatcctgt ttgtgacaaa cacgactcat gaaagtttgt atttcgaacg atatttcggt
    16801 tatcaaaaag caatgatgat ggctggtctt cctgcgttat ccagtgtagt actagagcat
    16861 ccggaggatt atctgtcctt tgagaaaacg ttgaaagaaa acgaagccac agcattggtg
    16921 gtcattgacg atatcttcgc ccttcgcacg atgcagttgg cgcaaatgta cggctatgtc
    16981 attccagaga cactctccgt cattagcttc aacaactcga tcttctcaac attaacacac
    17041 ccttacctta caagtatcga tatcgatatc tccgaattag gacgtgtcgc tatgcaaaag
    17101 ctgcgcgaat tgatccatga agaattgagc agtggcgtac gtttggtcat ccctcatcgc
    17161 ttgatcaaaa gagagacagt cgttccttta gaaaacccat actaaatagc tgactcacta
    17221 aatacgctga ccatttatcc tatggtcagc tttttatgta aaagaagata ctacttctaa
    17281 cacattgttt atctgtaaaa aagagcgtgg gacaaaagta aagaacactt ttgtctcacg
    17341 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
    17401 acaaaaattt aaaaagcaat tttcttgagt ttcttcttat ttcttggagt taaacgcttc
    17461 tgtcccagcc tcttttttcc ttccttcaat tttttacacg tcgacgattc aataaaaaac
    17521 cgcctaatat tgtaaagaca ggtgcaagaa tcgagaaaaa gacgaatggc aagtaatgca
    17581 acgcgctaac tttcatcgtt cccataatga atgtgccgct gactccccac ggtacgagag
    17641 aattgacggt cgcacctgaa tccgctagtg ttcgtgtcaa ataggttttt gataagccaa
    17701 cgtcatcaaa agaacgcttg aatgtttctc ctggaagaat gatggacaga tactgttctc
    17761 caacgatcag attgattccg atacagctca aggcagtaaa cccgactaaa cgagaaggtc
    17821 ggtccatctt ttcttttaat tcttgaacga tggtatcaac gatcagatat ttgatcagta
    17881 acccacctaa tgccaatgca agtaaaatca aactagcaga acctaacata ctcatgatcc
    17941 cgcctcgaga aagcagttca tctagctttg gatcaggtgt atttgcgaca taaccattca
    18001 tcaagatgtc tgctgctttt ccgaccgtca aacttttctc atggataaaa gaaagaacga
    18061 gagcagctgt cgatcccgct agtaatgtag ggatagccgg aatttttttg attgcccccc
    18121 caaataaaat aactagtgga atcaatgtcc aaggagagat ccaaaagctc gctttcatgt
    18181 tttggatcat ttcattgata ctagctagat cggctgaatg actgttatgt cctaatatcg
    18241 tataaaaaat caacgaaagg acgaatgctg ggaaatccgt ataacgcata ccagcgatat
    18301 gttcgaatag attaactcct ccaatacccg ctgccaagtt ggtcgtatct gataaaggcg
    18361 agcagttatt ccctaaaaag gcaccggaaa cgactgcgcc tgtagtcatt gcatcattga
    18421 atccaagtaa atgaccgatc cccaaaaaag caattccaat cgtcgaaatc gttgtaaatg
    18481 aactgccgac agtcactccg acaattgcac aaacaagaaa tgctgtcggt aaaaagaaac
    18541 gcacagaaac aagtttaaat ccataaacca taatcgtagg gatggttcca gataaaatcc
    18601 atgaagcaac taagacacca atcaaaagaa aaatcaggat tggaatgatc cctggagtga
    18661 ttcctttgat gatcccgtca tggatctcat cccatgtgaa ctttttaaat ctgccataaa
    18721 acaacaaaca catgaaagct gcaaggatcg ggatatgtgg cgagagctgg aatccgatga
    18781 tcagtactcc taggagtgcc aacaacacga tcaatacagc gatactttct tttaaagaaa
    18841 tatttttatt catcgtcgat ctcctatttc aaatgaaaag gccctgccat gaaaaaacta
    18901 ttcacttggc aggacctcct actaaatcag cgacaaaacg tcggctgaaa gacttatttt
    18961 ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt cttgaagtga
    19021 ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa taatgcatca
    19081 ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa gatcagcatg
    19141 cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg acgttcgaat
    19201 tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta cacgatacaa
    19261 taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc atccattaca
    19321 ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac atttctagat
    19381 acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc ttcacgagct
    19441 acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa aagcacacca
    19501 ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag gatttgacag
    19561 tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg tacgacattt
    19621 gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc gataaatacg
    19681 ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga actcatttcg
    19741 atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat atgttccaag
    19801 tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac tagtccgact
    19861 ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata tacccctagc
    19921 catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa gatcgtgatg
    19981 atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc aagttctaca
    20041 ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac catacctggg
    20101 aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc cagtgctgtt
    20161 tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc accggaaacc
    20221 attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat caatgtccaa
    20281 ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat tgtcagaccg
    20341 ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga cactctcctt
    20401 gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac gaaacaattc
    20461 agcgaatatt tgaggggatt ctttgagtct attgcttgaa gggtttcttc aaagacaacc
    20521 tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc aaacatgcgt
    20581 cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat tttgcttcgc
    20641 ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct gctttgattt
    20701 tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt acgctggcac
    20761 gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct agttggttca
    20821 caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt gaagttaaga
    20881 actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg tcatggttca
    20941 atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag tctaccatat
    21001 tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact ttaaatgaca
    21061 agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc attaatttac
    21121 catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg gctgctgttg
    21181 cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata tctgcgcctt
    21241 tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata tctttgatga
    21301 cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc gttacagatt
    21361 ctgcttcttc gattgcttta tatgcactgt gtacttcttc taagatataa tctttgtttt
    21421 ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga atgaagttgt
    21481 tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct acgtgtaagt
    21541 agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta tcgataccgt
    21601 cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt ggtgtttttt
    21661 cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga tagttgtggt
    21721 caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca gcttttagcc
    21781 atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa tgttttccgc
    21841 tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct aacagattca
    21901 tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca actagttcag
    21961 gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac caaagtcctt
    22021 ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta tagctcatta
    22081 attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga gaagattcat
    22141 aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa gatggcatca
    22201 atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga acggaatggg
    22261 tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca gtgtgttcaa
    22321 aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc atgtagtttt
    22381 gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag atttggccgt
    22441 tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac aatgattcac
    22501 tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca ttacacctgt
    22561 tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa tatgtatcaa
    22621 ttatccgaat tgttcacact tttgaaataa ttaaaaaaaa cgagggaatt tacaaatgta
    22681 aactccctcg caaagattgt attaacgaca accagcgttc tattttagga tgggtttcaa
    22741 aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta aattctaatc
    22801 aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa acttaccatc
    22861 gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac cgttaatact
    22921 tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa tttttgtgga
    22981 aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg gcattttacc
    23041 gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga acaatgcttg
    23101 cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg taacttcttc
    23161 tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt tcgctaagtc
    23221 ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta catcgcgatc
    23281 atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg gatcaagcca
    23341 aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca tcaatgggat
    23401 cgttaaaccg aacgcttcta cttctggtcc ttctaatttt cggattaggt ctaatccagc
    23461 tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt atgttttatg
    23521 cagatgcaaa aagtcgatag attgcagaat ttggtaagta aactctgtaa atgagattcc
    23581 tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt tgaagttttt
    23641 accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt agttgttcac
    23701 aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa gtgcgtccac
    23761 gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc cgcttgggtc
    23821 gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg ccaattggaa
    23881 tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac cagttggatc
    23941 aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc tttcttcgtt
    24001 tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca ttttactgac
    24061 ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta attatttctt
    24121 caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga cgaaatctcg
    24181 cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat ttaacgctgt
    24241 cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc atgtactggt
    24301 ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt ttcgatcttc
    24361 gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg tcaacagaaa
    24421 aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta attccgaaca
    24481 ttttattttc aattattgtt aaaagaaata actttttctg tattttttct gcaatttatc
    24541 gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa tgggacaagt
    24601 aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat tattttagga
    24661 gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt catccgatca
    24721 tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc aaaagaaccg
    24781 acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag ctacgattat
    24841 gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga aacacaacag
    24901 cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga tcctactcag
    24961 atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt ccactctcaa
    25021 caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg ttcgatcgaa
    25081 caactttatc aaaatgattt tgttttagtt aatttaaatg atttgatcca aaaagacgaa
    25141 caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc attggttctt
    25201 tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc tagccgattg
    25261 gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa agaagaaatc
    25321 ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc tgatttttct
    25381 tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt aggttatcga
    25441 acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa agaagctaaa
    25501 aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac atggggtcac
    25561 ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt gtggcagcaa
    25621 gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg tgcagacatc
    25681 agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa gagtcaaggt
    25741 ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt aggtacggat
    25801 ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga tttagacgga
    25861 aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa agaagcacgc
    25921 gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca ttttatcgtc
    25981 agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat caggacttct
    26041 gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga aattccttct
    26101 accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc cacttttcct
    26161 tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg cgtatacgca
    26221 tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat caaatcgata
    26281 taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat ggcaatcagt
    26341 gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc taaatcattc
    26401 ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata tttaaaaatc
    26461 gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc agcggttcct
    26521 atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt ctcttgtgct
    26581 ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt ataatatata
    26641 tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat caagctagca
    26701 attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg tactaaaata
    26761 ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta atcttgttta
    26821 gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa agtcacacat
    26881 ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac cgtacattca
    26941 ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac gattcggaca
    27001 cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca agatgtatgg
    27061 gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat ctctttgtgg
    27121 ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg aaaagggagt
    27181 gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa aaaagcaaaa
    27241 aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc tgttgattcg
    27301 gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga aacgattttc
    27361 caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc ttctcatatt
    27421 ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg aacagtcgta
    27481 gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac tatagaaaca
    27541 gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt gcaaaaaggt
    27601 cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc tgaagcgtcc
    27661 aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt attcatgaga
    27721 cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg gtccaccctt
    27781 ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt cggtgtcact
    27841 gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca aggattagct
    27901 ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc aactccagct
    27961 tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt gcttttttgc
    28021 agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag aaaaaaagaa
    28081 gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc aaactcttta
    28141 gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat cagctgtacg
    28201 atgatttttt tccaagcaga ttcgatcact gcagaaggtg cttggctttt tgggctatct
    28261 gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca actgatgcca
    28321 agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta ccttctacga
    28381 gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg ctggtcttat
    28441 ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt tttgttcagt
    28501 ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa caccggctat
    28561 atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt gcgtggttta
    28621 ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg tttgatgggg
    28681 gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa cgacttaatg
    28741 aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag cacgatcatg
    28801 gtcgtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg tttatttaca
    28861 gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag gaaccaactt
    28921 tattggacca atatgttact agctattcta tttgctatat tggcaatcct gattagttct
    28981 ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat ggatctattg
    29041 atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat cggaattagt
    29101 ggctttctat tcggatggct tcctcgaatg ggtaaacttg cttatatcta cttagcttac
    29161 tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt ttccaaaaca
    29221 gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc cgtgttcctt
    29281 acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta taggcgaaga
    29341 gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc caaatctggg
    29401 tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac tgctctttat
    29461 caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct aataatttaa
    29521 attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct ttcattttca
    29581 ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga tagacagcga
    29641 ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact tttgttaatt
    29701 tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt aagagttccg
    29761 agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg ccaaataaag
    29821 actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc tctattactc
    29881 gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg ccggaggcca
    29941 cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt tccacatagt
    30001 gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga ttgaacaaag
    30061 gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct aaacagctat
    30121 ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt atcatagcat
    30181 aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc ccttgcttta
    30241 ttttccttct tttgagacct taagtaattg attcctatca attttttaaa tatttacttt
    30301 ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt ttttcgcatt
    30361 gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat agtggtgcta
    30421 gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc ggatagaaga
    30481 atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct tcttgtttct
    30541 ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt tctggttgcg
    30601 gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt atcgcaagca
    30661 actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt tggtacatca
    30721 aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc tcgtcttgac
    30781 gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca ttcgtccagc
    30841 tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct gtctggagct
    30901 gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt tcaatcttat
    30961 ctgttggatg gctctctttg agctgtttga atgcgtagat tggtcgatca aacaactgat
    31021 aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt attctacctg
    31081 aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca agtcataaga
    31141 acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt aaggttgatt
    31201 gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg ggtcgatatg
    31261 tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca agactttggt
    31321 gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca atgcctctgt
    31381 gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc ctcggctttg
    31441 aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat caatagaatc
    31501 gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg gaccagaagc
    31561 cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc tgattgtttc
    31621 aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca tgttatttcc
    31681 atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt tctgattgat
    31741 tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt tcatattatc
    31801 acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt ctaaagaatc
    31861 tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca tatctagtga
    31921 ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt ccaacagagc
    31981 taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca ttccgttgac
    32041 tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag taacaagttc
    32101 ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct caccgtggat
    32161 accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc ctacttctgg
    32221 tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga ccttttctcc
    32281 cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca cgattttgtg
    32341 tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat tttcaacggc
    32401 gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag ctaattcctt
    32461 tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca aaactccttt
    32521 tccactatcc gcttccttaa tagccgtctg aatctgatct ggagttggag aagtaaacac
    32581 atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat gggatggttc
    32641 atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat tcttggctac
    32701 aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta atccttccag
    32761 catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat tcccatcctt
    32821 tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt ataccggcag
    32881 tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga taacgttgct
    32941 gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg ctaccgatat
    33001 ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct acgattttca
    33061 tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc acctcttcgg
    33121 atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga ctatgagaaa
    33181 taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg ctaacgattg
    33241 gtttcgcttt cattaatgaa caattcaagt atataataaa tttctaattt tgggaagata
    33301 tgtgtagtgc ttcttgaagt ttgttatact ttaattaaaa gagaggagtg atcgaatgat
    33361 tacaacaaca acgaacagtg tagagggaaa agccgtagta gcttataaag ggatcgtttt
    33421 tggtgaggtc attactggga tccatgcatt taaagacttg gaggcggggt tgcgaaatat
    33481 ctttggcggc cgctcaaaaa gctatgaaaa cgaactaatg ggtgcaagag aagaagcact
    33541 tgccgaaatg gccgaacgtg caaaagcatt aggagctgat gcgatcattg gcgtaaaaat
    33601 ggactatgaa gtattaggca gcgacaatgg catgctgatg gtcacatgta gtggaacagc
    33661 tgtctccacg agataagtag gggacatctg ttttaataac taaagagaga tgggacatgt
    33721 agtggcactc aagaattaga tttttcagtc taacttcgag gtactctacc ttcccatcct
    33781 ctctttttta tttctttttg cggatgattc cgtaagctgc tgcgactaac acacccgccc
    33841 ctgctaagga ataactgaca gaagctgttt tttcatttgt ttttggcaaa gtctgacttg
    33901 ttgatttgga tggctctgtc gtttgctgaa ctgtttctgt cgtgcttcca gaggtcactg
    33961 atttttctcc tgccgaatgc aaaatatctt ttattcgttg gaaactagct gctaagtcag
    34021 ctttttttgc ttcaaaatca ttagcaactg tttgtgcttc gctgatcttt ccgtggattg
    34081 cttgactggc cgctgtgata tccgcttgag tttgacttag ctttccagaa gctgtttgag
    34141 caacctgatt taattgctga ttgaattctt ttgcttgttg gatcttcccg tcaagatctg
    34201 tttggacagc ttccgctttt gctttcgctt cggcaagctt tttctcaagt tgtgcacggt
    34261 catttcccac tttattttta agatcagcta aatcttgatc gattgctgct tggtcttctt
    34321 tcaatttgtt ttttgcagct tctaatgctt cttcgagttc ttttctttta ttttccaagt
    34381 cttgcaaatg atcgatttgg tcagaagagg tagaactatt tattgatgta tcttctgaag
    34441 atgaaatcgt ttctcctgaa ttatctgtcg gtacattgga agtcatttct gtttccgagg
    34501 caccgttttc agcaccagaa gagcttatag ttgttgaagg ttctgtttga gcaggttcag
    34561 aagtttccgc aaaagctgtc tgtcccaata agaaaatact ggaaacagca attggccaga
    34621 ttaacggctt cattcttttt ttcatcactt ttcccatcct tactactttt taactaattt
    34681 cttattataa gcaaaaatgt ttggaagaac cataaacaca acaggctgtt tgtctaaaaa
    34741 acggtgcatc ttttattttt ttgtacgttc cgggcttttc catgtcccgc ttccataacc
    34801 taaaatcgtt ttgactgctg gaatgaaagt cgtcacgatc gttatcgcgt tcactatcca
    34861 gaacaaaagc atatatagtg gtgcgaaaag aaggtatttc aattttcttc gtcgatcatc
    34921 tacaatcagc gaagctagca actggaaaaa tcctgcgacc atttcaaagc agatgaacac
    34981 aaaactgaat gtaaaagtaa tataaatctg ttcgtagttt ccttgatata cataatgccc
    35041 gattaatcca gcaaaaagaa ctacggaaat acaaaagaat attgaccaga caatactcaa
    35101 tgtttgatca atgaagatga tcgttcttcc aatatgttta aaaggatgga gcatcacttt
    35161 tttgaaattc gtcagccaga cttctgttcc accttttgcc caacgttttc tttgtcgata
    35221 tagcatcttc aacgtcacag gtacttccat gaaaaagata atctcaggag caaaaacaga
    35281 taaccagtca tttaactgat gatcccaagc aatactgatg tcttctgtcg ctcgatcttg
    35341 tctgaatccg cccacatcga tcaaggcttc ttttcgatac atcgtatttg ctccactgta
    35401 cgcataaaga ccaccgaaaa cagctgactg tgttcgttta atgatgccca cgatacttga
    35461 gaattcaacc gtttgagatt ttgcaatcaa tttggtacga ttttggacat ccatatttgc
    35521 agtgaccgca gcaatatgtc ttgcaccagg gcgaatgaaa taattcacat acctaatcag
    35581 tgcatctggc tccggtaccg tgtctgcatc attgcttaaa atcaatttcc cttttgcaaa
    35641 ggcaagtccg atattaaacg cgtgggcttt tcctttattt ttttcgatcc gaaccacacg
    35701 aagattagcg tattttttca tcagtctagc taatatttct ggtgtttggt ccgttgaacc
    35761 atcgtctgtc actaatacct catagttgtg ataattcaat ttcgtcatca aatactcgat
    35821 tgtatcttcg atcacaattt cttcattatg ggctggcacc atgatcgtga tgaacggttc
    35881 gacactcaag ggaacatcta cccattcttt ttgcttatgt ttgtataaaa acacataaca
    35941 ccagacacca ataaaccata caaatccgcc taaaatcgga taagaaacga gaaaaaatac
    36001 ataatagaca aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca
    36061 gttccacccc attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt
    36121 cggagacaga ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat
    36181 acacttcttc aagtaattct tgtctttttt tcaatttttc atcttcatat tcaaaagatt
    36241 tatttacttt tttgtataga tttcgattgt tccacctagt caatacaaat gaggtaatca
    36301 ggaaactgaa aaaaatcaat acaaaaaagc cgattaagaa atacagcatt ttcttttctg
    36361 ataaaaaagc gtacaaggaa aaatccttgg ctctttctgg tctcgttaat gggaaaaaga
    36421 cccatataaa aggaagaaca acgatcatcc atcctaaaag agccactaac gtttgccaaa
    36481 tctttaacca gtaattacct tttgaaaaat acttatcttt caccaaaatt gatgcaactt
    36541 cttctcttgt tacttttttc atatcctcac ctcacgtttt tttgttacga cgtaggagtt
    36601 taccatacat ttcatttctt ttcacataaa gtgttcctaa atttttccta agaaaaagaa
    36661 caaaacattt ctcatcagca aatgaaaaat attttgttct ttttatcagt tgttcttttt
    36721 attttttatc tcttaattat gttgctcttt ccaatacgac aatgcttttc attcctctag
    36781 atatcgccgt gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat
    36841 ataagattac atgatcaaat tccagtcctt ttgctagact caaaggaatg atttggcagt
    36901 tttctaatcc atatgaattt tttttaatcg attcttgtag cgtttgggtt tcctcttctg
    36961 tttgggtgat aactgccgct ttttctcctt ttagagaaac caaaatctca gctagtattt
    37021 tcaacaattc ttcagaggat cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga
    37081 tcgatacgat gtcgatcttc tcttgatcca cagcataggt tttgaaaagc tctgtgattg
    37141 ctccgctaga acggtaacta tttctaagat cataccgatg gatcggcaga tggtagtcgt
    37201 cgaaacaacg cataatatca gcaaaagtag tggaagaatt aaagattgcc tgattctcgt
    37261 cacctactaa agtaaagtga gtttttggaa ataattcgat caatagacct aattgtgcca
    37321 gggtataatc ttgtacttca tcaatcaata gatagcgaaa tgctggtaca tttacctttt
    37381 caacaagtaa atgacgaata gctaatataa tgactgcttc atcaacagta cgtgtgggtt
    37441 gccaagcata agctgagcca tatcgttttt cataaattct ttcaaacaac tgatgctcat
    37501 ccacccatgc catttcttcg atttgtctgg ttatttttcg atattttttt tgtaaaagtt
    37561 tccttgcata agctacaata ctttgttccg aatcatcaga aatcaattta ccgaataatt
    37621 ccaactgcct atcttctgtc aacgagagta cctgattctg cacagcagga ctttttgctt
    37681 gtttcaaaag atgattcttc cattcgctca tcagtgcctt tttcgttgct tgaagcctat
    37741 cgatcaattt aggatgagca ggaactttct catatatttt ttcgatcgtt tttttagaaa
    37801 ggactgtttt tccattttta gtaagtccac ggataaaatt cggatgatct aaaaacaaag
    37861 gatctgattg ttttagatta tcgataaact ttttgctacg caaacgttct gtctgctccg
    37921 atacattttc tcccgtgata cgtttgaaat aagcctcttc accttctatt tcctctgctg
    37981 aaagctgtga aaccagctgt atgatggtta gattcagtgg atttctttca cctaatgatg
    38041 gtaggacatt cgaaatatag tcgataaacc gatgattagg tgatagaatc agcaagtcat
    38101 cggatgtcat cgtatctcga tataaataga gcagatacgc aatacgctgc atgatagcag
    38161 aggttttacc acttcctgct acgccattta ccaagatatt ttgacttttc acatcccgaa
    38221 tgattgtgtt ttgttccgat tggatcgtag cagtgatagc cttcatttca ttggtctcat
    38281 cttggcctaa gacttctaat aatacgtcat cttgaatagc tacagacgta tcaaaaaact
    38341 tgaccaaacg atctctttcc aaaagaaatt gtcgtctttc tttaataacc gtatcgattt
    38401 tattttgacg aaccgtataa gaagatggac caatcatgtt attgtaaaaa agttcggcaa
    38461 ttggtgaacg ccaatcataa acaagatttt catctgcatc atctgtgaaa tttgctgtac
    38521 cgatataaaa ggattctttt ggctcattat ccagaaagtc tacaacgact tttccaaagt
    38581 agggactttt caataaacgt tcgatctttg ctagctgatt ttctgcgttt ttctgtttga
    38641 tattcatctg atcgatttct ctatttttca tttcaatcat cgaaaaagta tccaggttat
    38701 ctaagtaact gtcaaaattc aatcgaagct cgccagccat ttctttcaac gactggtttc
    38761 cttctatttt tgccgtatcc atcgattccc gcagttcgtt ttttttatca attagctttt
    38821 gatacacgtt cttcagatat gcttcttcta actgtttttg tgtgttgacc atacacattc
    38881 tcctcttttt ttaaagtggt ggaaccgatt agtttacctc ttttaagagt cgaatgcaag
    38941 ctttttaacg agtatcattt gttaggatat atcagttctt caggtcggac agtttgatat
    39001 aaagaaaatg caccatcaag gttttttacg tggtagccag actgcttcaa gattctttcc
    39061 gctagatagc tacgtaatcc gctatgacag ctgacgatat attcttgttg gctgtctaat
    39121 tcatttaatc ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt gaatttgccc
    39181 ttttccaatt cttctgctgt tcgcacatct aataaaattt ttccttttgc tagttctgtc
    39241 ggcaattcat gccattgtac ggtttcacta agcccttctg caatgttcat tgctgcatat
    39301 cctagcatgt ttactggatc tttggctgag ccaaatggcg gtgcatacgt caattctaat
    39361 tccggtaaat cgaagatcgt caaatgccct ttgattgctg ttgctaaaat atcgatccgt
    39421 ttatccacac cttttgcccc aacaccttgt gcaccataaa tctcgccagt ctctggatgg
    39481 aagatcaact tcagtaaaat atctgttgct tctggataat agcttgcatg atcttttcct
    39541 gatacatgaa tgacttcaaa agacagttcg ttttctttgg ctgttctttc acttaaaccg
    39601 gtggatgcgg cagtcattcc aaacgttcgt ataattgctg ttccaatacc gccttgattt
    39661 cttcgagaaa ttccggaaat cgtgtccgca acttgtctac cttgacgatt ggctggtgaa
    39721 gcaagagaaa taagtgcatc ttttccagtg atttgctgtt ttacaacgat agcatccccc
    39781 accgcataaa tatcaggaat attcgtttga taacgttcgt ctactaggat acccccgcgt
    39841 atgcctaatt cgattccagc ggtcactgct agctggttat caggacgaac accaatggat
    39901 aaaataacta aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg
    39961 tcttcaaatc cagcgacagc atgagaagta atcacttcta ctccttgatg agacaattct
    40021 ttttctataa aagctgccat ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg
    40081 atcaccatga cttctaaacc tcttcttttt aggttttctg ccatttccaa tccgatgaat
    40141 cctgcaccaa cgatcacggc acgctttggc tgtttttcta atgcatgcat cactttatcg
    40201 atatctggta catttctaat agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat
    40261 ggaacaactg gttttgcacc aggagataaa accaatgcat cgtactgttc aatgtgtttt
    40321 tgaccctcat gtacgacttc taccgttttg ttttcgggaa agatggctgt cacttcatga
    40381 tttggacgca catctaaaca aaaacgagcg gataatgatt ctggcgtttg gacaagaaga
    40441 ttttcccgtt cactgatttc tcccgaaaga taataaggca agccgcaatt tgcaaaagaa
    40501 acatacggtc ctttttcaaa aacaacgatt tctgcatctt ccattaatcg gcgaagccgt
    40561 gtagcagcag acattccacc tgctacacct ccgacaataa cgattttcat cttatttccc
    40621 tcctactgtt cgatcaaacc attgattcat gccaccacgt acattcacta catcataacc
    40681 agatttcttt aattctttag ctgcttgttt actcctcatc cctgattgac aaatcacata
    40741 gacggtcttt tctttgtctc catcatatct gtttatactt tgtaatggaa catttttagc
    40801 tccttttata tgcccgcccc ggtattctgc aggtgttcta acatcaagta acgctggatt
    40861 ttccaaaagc ttttccttta attctgggac tgttatcgat tttattgatt gaaatagcaa
    40921 attcattttc tgcacctcat ttacattttt ataaatacct catggggtat attaaacagg
    40981 agctttatta atatgtcaac cagaatgact cttcattttt ttcactactt tttatatttg
    41041 cataaaatac cgataagggg tattatctaa ttataaaaat agaggagaat gtgttatgga
    41101 gattgataat aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat
    41161 ccaaaaaatg atcgaagacg aaaaagaatg tatggatgtc atcactcaat taagcgctgt
    41221 tcgttctagt attgatcgcg taatgggcat gatcgttgca gataatctga aaaattgctt
    41281 tgagaaacct gatagtaatc cagaagaaca agcggctaaa ctagaacaag cgatcaagat
    41341 gattattaaa aagtaaagga atgatttcaa tgaaagtcgt tttccatatt gatgaattag
    41401 aaaaatggtc agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga
    41461 cgattgtcgt atctgtaaat ggcatcgcga ttaccggtta tctagactct gccaatgcag
    41521 aattcttaga tttgcaagga gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc
    41581 atatttcaga aagttcttta cctgaacaag taatcgttgt accagcaggt gtccttgatt
    41641 tagttgaact gcaatcccaa ggttatgctt atatcaagcc ttagtcagta gaggttgcga
    41701 cagaagtgat cagcttcaag aaatttcggc ttatttttcg aagaagctac ttctggaaaa
    41761 ccgtgaatca aaggttgtga aaacgctgtc ctgcatcaag taataagaac agccaaacaa
    41821 aaatagcttc tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa
    41881 caccgtttaa ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac
    41941 gggaaaatag ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg
    42001 aacaccgttt aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt
    42061 ttgtgaaaat ggatttctac atttttgcat aaatttggct taataccgag aaactggctt
    42121 ttgaataccg tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa
    42181 caaacggaaa aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct
    42241 gattgaacac cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa
    42301 taatttcaaa aacaacttct cctgtttttg gtttggttgg cttattatcg agaagtagct
    42361 actgggagtc tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt
    42421 ctttgaaaca tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa
    42481 gtgatgacgg caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa
    42541 aggatcatgc caacatagat gacagcagtc gctactttgc cataccactt cgctccactt
    42601 aatttgatat ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc
    42661 aagaatagac caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga
    42721 aaaagttgtg tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga
    42781 ttatatctcc gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc
    42841 gctcctgcta tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa
    42901 atacggaata tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt
    42961 aatttcattt tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg
    43021 ggaataaaac caatgatttc actgttttta tttttcttta cagccactga tattatgagc
    43081 taatttgaga taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc
    43141 gcatttcttt tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc
    43201 gtttacgtgt tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat
    43261 cttctgctga acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta
    43321 aatatacatc aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt
    43381 cattgacaaa tatccgatca ccttttgcaa agagctggcg ttctttttta ttctccttcc
    43441 tatttgtaac tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg
    43501 ccaagtaacg ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc
    43561 acaaagcttg gctttctcct gtttctccaa cccgcaactg atatttagga cgaagtgctt
    43621 cccgatcgaa agccatagca gccggcacaa aatcttcatg atctccggcg aatttcttga
    43681 tttctccata atgggtcgtg gcaatgatca atgccccttg ctcatacata gattccatga
    43741 ttgcgatcgc tagtgcagcc ccttcatttg gctctgttcc actgccaatc tcatctaaaa
    43801 gtaccaacgt attccttttt atctttttca aaatagcagc gatgttttgc atatgaccag
    43861 aaaatgtact caacgcattt tctaaatttt ggtggtcacc gacatcgacg aaaatctcat
    43921 caaagacagc aagctctgtc ccttcttttg ccggaacttg cataccgaac atcgccatca
    43981 aagtcaataa accgactgtc ttcaacacga ctgttttgcc tccggcattt gctccggtga
    44041 taatcaagcc tcgatagtct ttaccgattt caacatccaa tggtaccgca tgctcaggta
    44101 aaaatggatg tctgccttgc ttgataatga tatgttccga tttattgatt ctaggtgtgc
    44161 ttccattgat ttctcggcta aattttgccc gagcaaagat gatatctaac accgtgatcg
    44221 tttcgatcaa taaatcgatc acttcttcgt tttctgcgat agcaccagtc aatgccgcca
    44281 ggacttgata ttcttcactg atttcttcag cttttaacaa ctgatagtgt tcattcaatt
    44341 tagaaatcac agttggttca ataaaaaccg tcgttctttt atttgattca tcgataatcg
    44401 ttccatctat tttattttta tagctggctt tgataggaat cgtatagtat tcgccttttt
    44461 gaacaatcat tgcttcttgg atcatttctt tgtttttcgg atgacgcaag aatttcagta
    44521 attttgactg gatttctttt tcgattagtt gtaattgttt gcgtacttta cgcaaattac
    44581 gtgacgcgtc gtcactgact ttattatttt ttattttctg gtcaatcaac tcttctacat
    44641 ttatcaaatc aggaagatgt ttgctataag caaacaacaa gggtgtctgg tattgattct
    44701 tatcaaaaaa ctttgtgatc atccgactgc ttctaagaaa atccgcatat tcgatcaaat
    44761 cggttggctg cagaatcaac ccttttttga cttgttctgt cagtgcatcg atcctcggca
    44821 accccataaa tggaacatgc tgactgcttt ctaagatcaa gcgtgcttct cttgtttctt
    44881 cttgacgatc caagacagtt tgtaggtttg tcgagaccgt catatcgctg attcgttttt
    44941 tgctatagtt tccaattgct cttgcttgga tttccttttt gatccgatca aattgtaatt
    45001 gactaatcat ttcgttgtcc atattctctc cttttcttga agagatacct gtcccaaaat
    45061 aaataagggc ctagagataa ataaaaggat agaacacagc ttaaaaaagt catgtcctat
    45121 ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa gcacaccaaa
    45181 taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac tcacaaaaca
    45241 taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac acattttggt
    45301 tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac ctaccgcgat
    45361 aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat ctgaagttcc
    45421 tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc gagcagggtt
    45481 gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca atccacctac
    45541 agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca acccaagaac
    45601 taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc gtatagcagg
    45661 acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc aatgtggcaa
    45721 ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg caatgatgaa
    45781 aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag agactgcagg
    45841 attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta cagcgagtcc
    45901 ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt ttaaattcac
    45961 agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa actctcccat
    46021 gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca gatctgattc
    46081 tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg tccattcaaa
    46141 atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat ctcgttcaaa
    46201 caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca tttcttcttc
    46261 taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag gtgccatttc
    46321 ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga tataagtagc
    46381 atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca ctgtttcttc
    46441 taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt cgatttcttt
    46501 cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag ctccttctgc
    46561 catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat ctggttctct
    46621 ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc tgttttccaa
    46681 atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat attcacttac
    46741 tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc caccattgta
    46801 atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg cttcgatatc
    46861 attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata gataatctac
    46921 atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat ctgtcgtacc
    46981 aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt gctttcctgt
    47041 atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta aatggatccc
    47101 tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc gtactttatc
    47161 tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca cttcatctgt
    47221 cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa agccaaccgc
    47281 tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat tatcaatcac
    47341 taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta gcccttcttt
    47401 tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat tctcatattt
    47461 ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga ccgagaacat
    47521 attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat ctggttttgg
    47581 gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag ctactacttc
    47641 cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg agcgagagga
    47701 agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg atgctgctga
    47761 ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat ccaattcttc
    47821 ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat ggatttcctc
    47881 cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa agtttttctc
    47941 ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat tcgcggatct
    48001 cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc cctagcgccg
    48061 ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt tggaattgca
    48121 tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc ttgattccag
    48181 tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac tgtaaagtag
    48241 ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc cctcgtgctg
    48301 cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa acctcatctt
    48361 cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac atttgaagtc
    48421 catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct aaagcatagt
    48481 aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg gataattgcg
    48541 gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc ttgaccatgc
    48601 ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc atccctgaaa
    48661 ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact tctgagttag
    48721 aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc tcctgatccc
    48781 aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa tcagtaacat
    48841 gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca aacatcaatt
    48901 cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag cgtatcttcg
    48961 ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat tcttgatagc
    49021 cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa acaactgcat
    49081 gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc tggttcgtta
    49141 tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct ccggcaataa
    49201 ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt tcttgcggga
    49261 aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg tcaaagataa
    49321 ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat tctgtcattg
    49381 tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac tactgtttta
    49441 aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt tttattatga
    49501 agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt ttcaatcgaa
    49561 taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt ttgtttctct
    49621 ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca atctcaatac
    49681 cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga taaatcggca
    49741 gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg atcatcaaaa
    49801 gaacattggc atactggatc atcaggtaac taagctcact atcttcagct cttttcctcg
    49861 gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag gaatgcccga
    49921 gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc tttccaacaa
    49981 agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt tcttctttga
    50041 ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa gtatctaaaa
    50101 cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt tcttcgtcta
    50161 ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca ttcagttctg
    50221 ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa agccggtccg
    50281 actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca ttcaatagtc
    50341 tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc gtaaacgttg
    50401 tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta ttttgcaaaa
    50461 aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa tatcgtatct
    50521 gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc tctaagagct
    50581 ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac gctgtcgcta
    50641 atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta aacttcaagc
    50701 tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc aacacaagca
    50761 gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg ccaatcaatt
    50821 caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc tctctcaaac
    50881 cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg atctgtaact
    50941 gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac ctttgaccac
    51001 accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc taactgttca
    51061 aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa catctctttt
    51121 ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa tttgagttta
    51181 actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc aattttttgg
    51241 ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata gccaaaccac
    51301 tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact aattgtcttg
    51361 ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat tttttcattt
    51421 gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt ttgagacgtt
    51481 tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg ttcttcccac
    51541 atctgattgt cggggcgaga atgattagcc agcaaaacaa gatcatagtt ctcctttctt
    51601 ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg ttcttctaaa
    51661 aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat ttcaggatct
    51721 agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt ttttcttgat
    51781 gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc aattttcttt
    51841 gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac tgggtgaata
    51901 agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc tagaatcaaa
    51961 cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt caatcctatt
    52021 actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc tttcgtgatc
    52081 caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa cccgctggga
    52141 atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg catcaattgg
    52201 cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac aatgttcatc
    52261 tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa aacacagtat
    52321 tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac cctctccatc
    52381 ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg aagagtatca
    52441 tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc gccttttacg
    52501 tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc gtgtgtaatg
    52561 taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa caactgcttc
    52621 gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat caatttgaac
    52681 cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca aagcatcttc
    52741 tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat tactgaaccc
    52801 agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg gcaacaatcc
    52861 tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt gtgaagcgac
    52921 tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac cagcataata
    52981 aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc ccaaggcccg
    53041 atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat tcaaaacact
    53101 ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat cgatcccatt
    53161 ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg taggacgctc
    53221 gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt tcccatcctc
    53281 tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa tgggcttggc
    53341 aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc ctcacggttt
    53401 tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat tgctgcaagt
    53461 tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc gacaccaact
    53521 aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt atcgttgtat
    53581 ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata atctagtttt
    53641 gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc tggtgtttca
    53701 tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata ttgttcaacc
    53761 gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc caagctaccg
    53821 ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat caaatacgta
    53881 tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg tgtggctttg
    53941 ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg tgtttccgca
    54001 tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga taaagttgct
    54061 cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc ttctccttgg
    54121 attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat gctgagttca
    54181 cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac agcaccaaga
    54241 cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc attgaaaaag
    54301 ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac tcctgaaggt
    54361 aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg ctctgattaa
    54421 cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa ctaatttgct
    54481 taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct gggctaattc
    54541 tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt cccagctacc
    54601 atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg acaagcgacc
    54661 ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac gagcaatcgt
    54721 tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat ctttttcttt
    54781 taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa acgcttcatc
    54841 agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac attcatctaa
    54901 cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat catagtatga
    54961 tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta gatttgaaaa
    55021 aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc ttctggaaca
    55081 ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa aattttgtga
    55141 aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg gcttttgaac
    55201 accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag aacagccaaa
    55261 caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc agctgattgt
    55321 acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa ggagcaattt
    55381 caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga gttggcagga
    55441 gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag tcccttattt
    55501 ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa tcaaatgatt
    55561 ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca tagatatatc
    55621 cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag ccccacgacg
    55681 tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca atatgctgtt
    55741 tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact ttgtctatag
    55801 ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat aattgattca
    55861 aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat ggaagatacg
    55921 gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt tctggatgag
    55981 cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg taggctgcta
    56041 agatttcttc atcacctact tctattttta agttgacacc tttttcttca aacattcgtt
    56101 gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa gaagttcttt
    56161 gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca agaagctgat
    56221 agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct tttgtagcta
    56281 aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc aaatggggat
    56341 ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag ttgatctcat
    56401 tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt tcagcgaaac
    56461 gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta accaaatata
    56521 acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt agacattcgt
    56581 caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca tcaccatgag
    56641 gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt tcagcaaata
    56701 aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta ggataataat
    56761 acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata tgccattgat
    56821 ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac acagataatc
    56881 ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct ccgccccata
    56941 aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt ttcttgtttg
    57001 attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg taacaataac
    57061 cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt ccatcaataa
    57121 atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt tgccatttaa
    57181 attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac tgactacccc
    57241 aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacaggac tgtagatttt
    57301 tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa atgttggatc
    57361 gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca ctatatcctt
    57421 tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca tacaggaact
    57481 gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa tataaatcgg
    57541 gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg caccagctac
    57601 tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat atttaagagt
    57661 cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg cagataaaga
    57721 taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg ctcctgcttg
    57781 cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga aagaaagatt
    57841 gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca cttgccaaaa
    57901 tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta aatatccagc
    57961 tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaag taattgggac
    58021 catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt gcaagctttt
    58081 gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg gaataaccgt
    58141 acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta tgctatcact
    58201 atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag cgatgaaacg
    58261 atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga aatagaaaaa
    58321 tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta ataaattcaa
    58381 actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga cgcctaaagt
    58441 cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt ttttctcagt
    58501 tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata atacgtcata
    58561 cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt cgactacagt
    58621 gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat cttttagggt
    58681 aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc ctccgacaaa
    58741 tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct tgttaattaa
    58801 tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt tctccattca
    58861 attagtaccc cttataatag cgaacgaaac tgtacaatag tgtgtatggt ttacttagcc
    58921 gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat agtcaaagga
    58981 gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct caagctgata
    59041 ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag tctcggcatt
    59101 taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg aatctatctt
    59161 tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt ttgttttctt
    59221 tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc aagattcaaa
    59281 ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa gctcttgctc
    59341 agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga aaagatacac
    59401 tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct gtttttttag
    59461 tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt gttttcgaaa
    59521 aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta aattgggacg
    59581 taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag gataacggga
    59641 gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc tcgttttcat
    59701 ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa caggaagatc
    59761 taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt ctttgttttt
    59821 ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg cttgtttttc
    59881 ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg tctctaccat
    59941 cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc gaagaatggt
    60001 caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat aattatatga
    60061 tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa aaattgtact
    60121 gatttgttga ggcaaccttg cgtgttggat caccgtttct gttccaacaa gagataagaa
    60181 aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg aaacttccag
    60241 ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta atgatacggc
    60301 tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc ctttttgaaa
    60361 caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga ttttatgttt
    60421 cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta aaaaataacc
    60481 tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca tatttttgat
    60541 ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt cgcttgttat
    60601 ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat ttaagtccat
    60661 ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat gacaatagtc
    60721 acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt ttaccattag
    60781 actggataat ttgtttataa tattcaaaac tatctttttt caatctccta aagcttccgc
    60841 tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt tcccctgttc
    60901 ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg caaccatctt
    60961 ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt tttggatcgt
    61021 ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca actatgaata
    61081 atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct agaggatcaa
    61141 ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg aaaccatttg
    61201 ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta cgataataac
    61261 taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca tctaacatta
    61321 ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa tgccctcggc
    61381 acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca aatacattct
    61441 ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata atacccatct
    61501 tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct agaaacatat
    61561 gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca agtttatctc
    61621 tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca ttattgatct
    61681 cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg gtctgcgcat
    61741 aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct cctaccaatg
    61801 ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat cgatttaatg
    61861 tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt tcgtctcctc
    61921 ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct aactccttca
    61981 gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga gaaagataat
    62041 ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac tgaatactag
    62101 gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg ccgtcttcca
    62161 accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa ttttctggaa
    62221 atatttcctg atccataact atctctcctc gctatcaaca atttcttact tgaagtatag
    62281 ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa aaaaattgaa
    62341 tgctactctt catttttaat atttatatat aacaaataca taagacgatc cccgaaagaa
    62401 aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa tgcttcataa
    62461 ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga cacaataacc
    62521 cttaaagctt cacgtggaac agtgaacgtc tgcttttcac tggacatatt acaaataacg
    62581 agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg cgtcttttcc
    62641 actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc gatcaatttc
    62701 ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt gatttcttta
    62761 taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg ttcacgggcg
    62821 ttccattgca taggcgtcct agcattgtct cttcctttcg cattgattga ttccaagatt
    62881 tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt ctcaatatct
    62941 tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc tccttgatag
    63001 atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc actttctttt
    63061 cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg attattccaa
    63121 aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa tacttttttc
    63181 agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg ctgatctaat
    63241 cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt ggaataaagt
    63301 ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt tttgtcacca
    63361 aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt acttgtgatt
    63421 tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc cccaatacct
    63481 ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt atttttccaa
    63541 ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt tttttcatcc
    63601 aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt tccattgacc
    63661 ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg ggcctcaatg
    63721 aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat acggatattt
    63781 cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa ttctgccatg
    63841 attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc gtatactgga
    63901 gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga aataatcccc
    63961 cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata gatttgataa
    64021 acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat ggatgtttct
    64081 attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg ctttcaaata
    64141 gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag gaaattccct
    64201 caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca caacttcttt
    64261 ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca aaatatgagg
    64321 aactattttt tcgttttttt ctctaatact caggccagaa cactgatttc acaacctctt
    64381 tataaacggt gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag
    64441 aaactatttt ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct
    64501 ttatttcaca ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat
    64561 tggagatact tcttttcctc ttttagctgc taacatggta attggataga agataaaacc
    64621 taatgcaatc ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga
    64681 agggatcgca atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac
    64741 tcctaccata atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga
    64801 aaagaacaag cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc
    64861 tccaacggcg ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa
    64921 ggaacccgca agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt
    64981 gttgtctttc atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa
    65041 gaaagtcaca agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc
    65101 tacatgtttt aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc
    65161 agaaggcatt ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ctatcccgat
    65221 gaagatgcca cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat
    65281 cgtcaaccaa gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga
    65341 aacgatgatc ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga
    65401 aatcgcatat ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat
    65461 tgtgatcaaa ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg
    65521 aatccccatt cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc
    65581 cgttccaatt ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa
    65641 tgcagtcgca gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt
    65701 tacaaataag atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt
    65761 taccgtggtg ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca
    65821 tccccctaaa tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac
    65881 aacgtgtttt atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc
    65941 aaaatccgaa cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt
    66001 tggattacaa gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc
    66061 aatagtgtga tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat
    66121 cgacaggatt cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt
    66181 ttgtcttctt ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc
    66241 gccgatactt caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt
    66301 attcaatcag acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa
    66361 ttgaaagtca agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag
    66421 atagtttcaa attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt
    66481 gaaaacttct gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg
    66541 agtacaatac tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt
    66601 ttttctacaa agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt
    66661 ttttgtatca tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat
    66721 tccattatat cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc
    66781 aatagtttct aggattgctt tcctccacaa acttttttta caaaaaaaaa aaacggcaat
    66841 gaatgccgct cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta
    66901 gtgtgtgtct aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga
    66961 ttgcctgtgc gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg
    67021 cttgtgtttc ttccgtcagc attgttcttt gggcttcacg ccagttgaga cagcgacaga
    67081 tcgctccttg ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg
    67141 cacctaaagg caggaatgat tcgccacctt gggcttttcc taaatgcaga tttcctgcga
    67201 tcttttcgat atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga
    67261 tatctacaag cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag
    67321 cttcaataga agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact
    67381 gagcaatgac ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg
    67441 ctgcctcatt caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga
    67501 taccatttac ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat
    67561 ccactattac ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag
    67621 atagtctgtt tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc
    67681 ttttttacga taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc
    67741 tgttttttgc ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc
    67801 ttgatgttta aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat
    67861 ttcctcagat tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat
    67921 actcactagg aaaaaatcgg tttctttatc ttttagctct tctgtaagtt tttctagtgc
    67981 ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa ttatcttttc caaatgtatc
    68041 tgtaaatgtt tcgatactaa tttgttgtag taagtctaca tctgaaggcg tcatttttcg
    68101 aatttcgtat ttattcacgg cttcttcctt tcaatagatc cttttattgc cttttttgac
    68161 aaaattccaa tcttcgctaa cattcttgca tactttattt aaaagatatt ccaattgttt
    68221 tgcttcttcg tcagaaagac catttaatgc gactgcattg gagtagttgt tttctcgaat
    68281 gatgaaagga tagatttctg ctcctttttt agtcggatag agtttcttga tttttttatt
    68341 ttctttatct tccaaacgtt cgatcatccc attggattct aatttcttga ttgctcgggc
    68401 agtcgttgta cgatctactt tgatcatttc tgctagtttt tcttggatga ttccaggatt
    68461 ttcgcaaata cgtactaaat aaagatactg tcctcttgtt aaatcgactt ctttgaattc
    68521 gatattgcta atagaatcta gcgctcgggc aatcattccg atgtctcgaa ggatttcagc
    68581 catttcatcc cacctttatt ttgtttgtaa gactagttta cctctttttg ttgcaaatgc
    68641 aactattttc aataaaaaaa tcaagttctt ttttaataac tagtaaagta tattcttttc
    68701 ttcttttacg aaaaagctca atcacaatag atctggtttt tgttttctaa aataagtaac
    68761 tgtttcttcg ttgttcgctt ttctgatagc tttggcaact cttctatact aaaaaatccc
    68821 atgttggaag tttcattatt ctcaataaac ttggcctctt caccgtggat cgcacaagca
    68881 aaaacaagtt tataatactg aaaaagctgt ggaatatctt tttgtttatt cgtatcaaaa
    68941 actgctcgca actgagtagt ttctactgtt aatcctgttt cttcatagac ttctttacgc
    69001 acattttctt caggagacaa tcctatttca gcaaatccac caggaagtga ccattctttc
    69061 gtccgtttat cttcgattag taggaccttg ccatcttttt tgatataggc acgaacatct
    69121 acttttggtg ttggatgtcc ttctccaatc gttgtgagcg cttttatttc ttcgaaagtt
    69181 tcttttccta tttcactgat cagttccaat gaaatcgtcc ggatctcctc aaaacgctct
    69241 ttatcaaata catcttttcc ataatataga ccagcttgag aaagtgcgag tagtcttttg
    69301 tattgagcca acttcttgtt cattctcttc ccttcttttt tagcaaatgt atgactatgt
    69361 aatcaagtat agaagactgg tttaattacg gcaaataaat ataatgaaat ctgcaataaa
    69421 taaaagaatg ttaaaaaact cgagaacaaa agacattcga cttctctggg gctgtgaata
    69481 aatggcgttc aaaagcttgc atctgcggta ataagcccca ccaaacgaaa aatatgacga
    69541 gctattcttg tctggctgtt cttattaatt gaggcaggac agctttttca caacctcccc
    69601 actaaacggt gttctcctac caactcctcg gtcacaagtc acaatattca tcaaacatga
    69661 gaagctgttt ttttgaaatt gctccttgtg actcggagct gaacgacagg ctcacgacct
    69721 ctcggtaaac agcgttcaaa agccaatttt tcgatattaa gccatttctt tgttacttat
    69781 tgtcattgat ttctttttct aatttatcca atagtggagt gtaatccact tgagtatagt
    69841 ctgttacatc aaattcatat agtgttccta actggaattt gtcatatttt cggtcggcga
    69901 tgatcttttt gaatgcttct ttcgtaatat gattggtccc tttcgttcga tcttccaata
    69961 cgtcaccata gtgataatga tccatgatat agctcaaatg acgatctgat tcacgatcac
    70021 gtttgtaccg acgttgacat aacacttcaa agtctgctgt taggcgaatc gtgatcacct
    70081 gatatccgta tctatctgct aattcttgga gtcgtgcttt ttgcttatca ctaaaaggat
    70141 actcggacac aatcactttc tttcctgctt ccatatacaa atcaagtgct ttgtaataga
    70201 aaggataaac tttcgtcctt tcttgttccg cccgttcttc caaattgtta aacccgatgg
    70261 attctgcata gtattccttg atttcatcga gcgtgatggc atacatatct ggaaatcgtt
    70321 cccgtagttt attcataaga tatgtttttc ctgtaccagg actaccagcc agaagaatga
    70381 aatacttttt catctcattt atttcctaag ttaattggac tttccaagtt cataaacagt
    70441 ccaatcttat gcttgacaat ttcttttact gcttcatttg ctggaatcac attatggcga
    70501 agagacttgt tgatttctgt ttcactaaag gtttctttag ctactttcgt ccagttgact
    70561 aaaagctccg ttccaacatt gaatttgcgg atcccgttat tgatcaattc aggataatct
    70621 tcttctttca cacctgttcc tccatggata actaggggga catcaactac tgcatcgatt
    70681 tctttcagca agggaatgtt tacttctgtt tttgatttga attgtccatg attcgtaccg
    70741 atagcaatag ctaatgcatc cacgcctgta gcttctacaa atttcactgc gtcttccggt
    70801 cttgtatata ccttgtcatt ttcagcgaca tggattcctt cttctgtacc gccgattgtt
    70861 ccaagttctc cttctacgga aacaccgtgc gcatgggcat attctactac tgcttttgtc
    70921 ttcaggatat tttctttgaa aggcagatgg gacccatcat acatgacaga agtatatccg
    70981 ctgtcgattg cttctttgat atcatcaaag tctcgggcgt ggtctaagtg caagactgca
    71041 tcaaccattt cttcttctgc aatagctgtc attacattga ctaacacatg catgccgata
    71101 tatttagcgg tatcgacact tgtttgaata atgatcggcg aacccatttc ttttgctgcg
    71161 cctagcatag caggaagcat ttctaggtta tgcgtattaa aagctccaac tgtaaaattc
    71221 aactcttctg ctgtttttgt tacttctttc aatgttgtat acatctttta ttgccacctt
    71281 atctttttat ttgtttcctt tgatcgttac ttgttttgca atttgtcgca tctcatccat
    71341 tttgcccatg tagtaatcga tcccttgatc atcaccagca cgagcagcat ctgcacgctt
    71401 ctggttatag aggctcatca gttttttata cccatctccg atcgtctgtt tattttccaa
    71461 actcttttct aaatattgga tggcttgatc gttcacatac aattcagaaa atttaagtcc
    71521 aacttgttca taaagtttcg ctaatttatg cttatcttgg gtagtggaaa tctcttgttt
    71581 caattgtgta atctctgatt gaatcttttc ggtcgcttct ggcgataggg ctttttcttc
    71641 ttttgccaga acttttttcg gttctttttc ttttttctta aaaaatccaa acatcatttt
    71701 ccctactttc aattaaaatg ctttcaaaac tgggcttaag atccaggcat ataagagtgc
    71761 tgcggctact gtatctacat cggttgctgt agcattgatg aatcccatcg aattaaagat
    71821 cgttactagc aaagcgggca ataatgtaat aaagaaacca tgagcaatac cgccaatgat
    71881 cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct gctgtccctc cggcaaagaa
    71941 attggttagc ataccaggta aaatcatcgc taagccgaac atgggcaaag taaacatggc
    72001 aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg acagcatttg ggctataagg
    72061 aaagaaaact ggacaatcta aggcagggat cgcatcagga actaatttca tagctatccc
    72121 tcgaaatgcc ggcacgattt cacctaacag tagacgtaca ccagctagca atacatacac
    72181 acccactacg aattggatgg cttgtaaaaa ggcaaacatg atatagtttt gatctcctga
    72241 taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg atgatatata gcgggaccat
    72301 cacgaccatg acagacaaat aagtatcttg cagaaattcg aaatgtgtcg gcaacttgat
    72361 atcttcgatc gactttttat tttcgccttt ctcaccaaaa agcttagcaa caccggcttc
    72421 aaataagtac ccgatcgtac aaaaatgacc taaagcaata tcatttgatc cagtgatttt
    72481 acgaatgatc ggttgcgcta ctgccggcat agcaatggcg aaacaagctc cgataaaacc
    72541 accgaccaag atcaatacga tccctcgcag accggcaaaa tagccaaaga ctgtcgtcat
    72601 cgttgccatc cataaaattg cttgaccagt taaaaaaatg tacttccatt ttgtgaaacg
    72661 agccaaaata atattgaata caaaaatggc taagaacgtc agtgcaatat ctctacctaa
    72721 acccaaatca ttcattgctt gaccattgat agattcaata gaagggatga tcccttgcat
    72781 atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa cttccaacga tgatgctcga
    72841 accggcactc aacacttgaa atcctaggag tgtctttaac gtgccagaga tgacttgtcc
    72901 tgctgatttc ttctgcagta gtagacccaa catagcaatc aatgcaatgg tgatcgatgc
    72961 ctgagtcaaa atattttcga tgataaaatt gataacgccc atgtttttac cctcttttct
    73021 ttttgtattt agataactcc cttatcttgc agaactgggg tgattttttc ttcaatttca
    73081 ttttttgaaa caatattttt caaatagatc atcgccgttt taggatcaac tgcgaacttt
    73141 tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat ctggtttcat ggaaactgcg
    73201 gaagaaatat cacaatggtc cagtttggct tctactccta atttgttcaa aacatcctca
    73261 gcagacatct gtgctgcaaa actacttcct aaacctgcac cgcatacgaa taaaatgttc
    73321 aatttgttca tctttgttct cccccgtatc atttattatt tctcaactac tgaaaaaagc
    73381 agttgtttga aatcttcgat atttgtgcat tcaatcaatc gatccaactt ctcttcgttg
    73441 ttgatcaatt caattagttc tttcatgatg ttcagatgcg agtaggagtc tactgctgcc
    73501 aaacaaaaaa taatctttac tggatccatt tcaggattac cgaaatcgat tggttgctca
    73561 atcgtagcta cgctgactcc taatttattg accccatctt ctggtcgggc atgggctagt
    73621 gccatgtgtt tgccaatcac gatatacggt ccgtaatcat tcactgcacg aatcatcgct
    73681 tcgacatagc gctcttcgac aatgttttct ttaaccaatg gtctagcagc tagttcaatc
    73741 gattcctccc aagtagtggc tttttctttg atcatgatat tgctgtccgt caaaatatct
    73801 tttaacatag gctggatctc ccttttgttg atttctaaat tgtttgtttc gaataatttt
    73861 tctaacttgc catagatgat tcgatctact cggccaccac tttctttcat gacatctatt
    73921 atttgagtga ataactccgt cgattcatga ctgttaggct ctaaccgctg tagttcttca
    73981 tttcttttca aaaagtcttc tataaccaag atattcgtct cagttaaaat cggatcgaca
    74041 actaaaagcg gcttatttcg ataattcaat tgaaaggtgc tgaaaaccag attgacatcc
    74101 aatttatcta ctaattcgat ttcttttgaa cttaaaactg cagtaatctc gatctgagga
    74161 aacttttctt tcagattctc tgctaacaga tttccagtag ccatcccatg gttacagaca
    74221 acgactgatt tataaacata attcaagtct cgcttgatta cactggcgat cgtcgaaaag
    74281 tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc caccaattac ttcttcgatc
    74341 gtaggaacaa aatgagtgac tgcgcggtag attgggaaat aatttgttcg gatgttttct
    74401 ttcaatggat tcatgacttg gatatgattc ttgattctta caatcagtgc agccatgtgc
    74461 ttatagagat tttcgaataa agcttcttcc tttagatgga aaggtacgtg tgtctcgttc
    74521 tctacaaatt ggatcaattg gatcgttagt aattgagctt gtacccactc gattgaattg
    74581 gagatatctc tagcattgaa cgtctcaata gtgaaacgaa gataatttct ttctacttgc
    74641 gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa taaatgcaga aaagctactg
    74701 tcttttattt ctgtattttt ccaattaatg gcagagataa attcatgttt cctgattcgc
    74761 tggatccaga tcaatgtaaa aagcaggatt tgttttttat aaatatcatc ttcttgtttc
    74821 aacaacttat tggaatagat ctcctgtatt tttttgattt ccaccaaagg aaaataatgg
    74881 agaaagattt tttgagccgg tgttaattgt gcatcttgat tcataaagaa gtcggtcatt
    74941 cccagattct gattgataaa atcatagatc atcgtacgga tagatcgttc tgaaccttta
    75001 taaaccagtc cttgctttcc ataactgacg atctctatcc cataactgac cagtgttgct
    75061 cgtaaccgtc tcatatcttc atctaatgta cttttagaga tttgaaattc ttcttctttt
    75121 ttgtttagat ggatcgggat tcggctgtaa gcaacggcta aaactagatc aaaaatacgt
    75181 tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt cagtttctaa tcgagcttgc
    75241 ttttcctttt ccagcttcaa caaaaaaact ttgttgcgaa ttgtacggat ctccggaagc
    75301 tttttactgc tcaaccagat attcgcttca agaacttcct gacggatcgt ccgctgactg
    75361 acattgaact cacttgctag atgttttgct tttactggat attgggctgt catgattctg
    75421 tgaagtattt caattgttct tgttttcata gaaatgatct ccttctgatt tcaagtatat
    75481 ggttccgctt acataataaa aagtagatat ttctgccact tgaagtggaa agaaagtaag
    75541 caaagcaaac aaaaaataag ccatatgctt tttcatctcc tcaacgaaaa agacatacgc
    75601 ctattattac aaaaattttg cccatctata caaaaaagag gttgtgaaaa agctgtcctg
    75661 catcaagtaa taagaacaac taaactaaaa tagtgcttcc tatttttcgt ttggttgttc
    75721 ttattatcgc agatgcaagc ttttaaacac catttagtgg agaggttgtg acagagatgt
    75781 tcagccttga gcgataagaa gcgattttga aaaatcgctt cccatatttt acaaaatcgt
    75841 gccttattgc cgataggcta tctctggcac actgtttatt cagaaaaagg agctgcgaca
    75901 aggcttttgt cacaactccg ttcataaata tttcctgtca atccagcata atcggctgaa
    75961 taaaatcggc ttgccatctt ttttctttct cagaaaaatc aaaataccat atctgagaaa
    76021 aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc atcttttcga tcgactgaat
    76081 aatcacagca tctgaaatca attcggatgg caaaactatt ttcgtggatc tgtcgataat
    76141 ttcctacttc ctgaatagtt atttttttag tatgatttct tacaccgtct gctttgtttt
    76201 tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt ataacagctt ctggcttttt
    76261 caggagtttc ttcttcccaa gcggattgga tcttcatgaa tatcttttgg atttccagaa
    76321 actgtgcttt cttttcttta gccgttcctg acatgcggga caatatctcc gctttcagcc
    76381 ttttcttttg tcttttattc ttgacgatag aaaaagcaga aacccctcct actgacaaaa
    76441 cagctaatgc aagaaatccc gtttgctcat aactagaagg acctgtacct ggaccataat
    76501 acccataaga atctctgccg taagaacgac tgccaaaata actgccccca tatgaacgac
    76561 caccaaatga acctctgccc gaacttcgac cgctagatgt acctccacca tgaccacccg
    76621 cgacagcgaa tccttgggtc ggcagagcaa acaaacatag gcccactaac aaaatgattg
    76681 ctaaataatt gatttttttc attgtcttcc tcgttatctt ttattacaaa aaactatttt
    76741 tatgatagcc ttgctaaaag gaagagtcaa taaccagatg cataaactgt ttttttagca
    76801 attttgtctt atttagtcat tgagtaacat cggacaaatg tatgtgtcgg cagtccataa
    76861 ttcctctatc ttcagcatga acaagcttgc agacaagaga tctgcgcttc ctccaggaga
    76921 gatttggttt tcctgacaaa agtgcaacag tgctgtaaat ggacgatttg gtaaagcact
    76981 ttgactagca gcatgagcaa gtttttgcat ttccactaac gttttctgat cactgcgatg
    77041 caatacgcat gtgtcatcta gcgaactcat caaaactaac aacatatgca tttgtttctg
    77101 tacatatgaa tcattatcta gctccttagc tgcttggatt gcaagcctga tatgcggata
    77161 ccctaatatc gcttcttcat atgcaccatt tacgccatat ttttgcttca ccctatgacc
    77221 atgagtggtt tttttatgac gatattttgt atcaggaaaa gcagcaagct tgctgacatc
    77281 atcaagtatt ttcgataaag aaaaaggctg ttccatactg attttttgtg caatgacact
    77341 gatcaatagc cccattgccc aaatcgctcc tttatgggta ttgatgccat ttgtagcaat
    77401 cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt ctttctctta actcctgatc
    77461 aatagcatga tgccatgcta tttgagccat ttcttcaaaa aaaggagtta gcgtcttagc
    77521 agacttaaga aataaagcat agtccatatc ggaatgtgca cccgcatcca gtgcatccac
    77581 taatccaggt tttggactta atgttacttc tgcgatcaat gcttcctcta ccaacgcagc
    77641 gagttgctga gtcactgctt tttttcgttc gtcatttatc atgacactca ctccttcttc
    77701 ctactattac ttgctgaaac cttttttgat agctacccaa atgatatgaa gtaaccagct
    77761 taaagcaaat gcacaacttg ccgtcattag ataagctaca agaatcatga ctcctgaagg
    77821 taactgcagc caactatctt taaacaataa taacagtact tggaaaacaa atacattggc
    77881 taaatacgta cgataagcat aaacagacaa ccatttgaac aatggcaatt ttggcgaatc
    77941 gtttacaatc attttggaag cgcctatgaa gacagcaaag atgatgacta aactgtataa
    78001 actttggatc gtattcaagt aaggtgcatt cgcaaagctc aagtctcctg gatgtgcgag
    78061 caatgaacgg acagaaaaga acgcgataac taaggcaaca ggtaagaaaa gataacgtac
    78121 tctgtccaaa tattgataga ctttttcgtg ataaacaaat gcggctaccc cataaatcat
    78181 gaaactagca acaaaccgat ctaataagta ccagctgtca tgataaggcc cagtcaatac
    78241 ttgtgtttga tagaacagat accatgcgac atataaaatc gttgaaccaa tcaatactgg
    78301 ccatacatat tttttattag caaatacttt gtaaccgagc caaacaaaaa acggcatgaa
    78361 caattggatc tgcagcatca taacggtata ccataaatga ggcgctccat cacctgtgac
    78421 atatttcagt aaaaagcttg cagtatccgt atacggagtt tgttgttgga ctgctggaaa
    78481 tagccaaaga taagccgtcg tccatagaag atacggaacg actagttcaa aaaacttttc
    78541 tttcaaaaat tcccaataag acaaatgctg acttgttttt accatattat aaacaattgc
    78601 aaaaataaac attggtgctg tgtatttaac gatgacgtaa aaggtaccaa gaaaagctgt
    78661 atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat atcgtttgca tcataacagc
    78721 cgttacagct gatactttct ggaagtcact tacgttgatt ttgatttttt tgttttcaaa
    78781 catctctttc tctctctttt tctttattac caaagttctt tggttaattt aggtccttcg
    78841 actgttttca atagaatttt tttctctggc tctctcaaat actcttttag tgaaaaaccg
    78901 ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa aagcaggatg cgattgaatt
    78961 acctgaattg gcaattcaat cggtgaattt gcgtacagca gcaaatcaaa atcactggtt
    79021 tcttttactg ccggaatccc agtcgataat tcaaatccta agctgccgcc gattccccat
    79081 ttacatcctg ataatatgtt tctagcagca tgaaattgct gataaacagg ataatccatg
    79141 attttctctt gtctatacga ttcttgttcg atgatgtccc acggatgaat gacttgtttg
    79201 acggcatgtt ttgagatttc tgttgaccat ctctgattgc gggcaggtcc acgtaaacca
    79261 atcggtagta tttctgcgtg atccgtcgac gcacgacgaa ctgttccgaa catcttttca
    79321 gttagaacga cccattcagg caaggtgaac ccttctattt catgtttatc aaaaaggaca
    79381 atatcatgtg cttgaatcat tttttatgca tcccattctt cgtccatttt ggcacggact
    79441 ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt ttgtgtatcg atttgtcagc
    79501 atcgtgtcat ttgtattgcg gacatcttga atggcttcat tgattgcttc gatgactttg
    79561 ctgacatttt gttctgaagc agcttggtcg gtgatatctt ctaggaaatg atagatcgct
    79621 cctagttttg tataattatt gatatcgtaa gcgatagctg gaactttttc agcagctgct
    79681 tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac tttctttaga catcgcttgg
    79741 acggtaattt ctttattgtt taaagcaatc aagcggcttg attgtaatcc atgtgctaaa
    79801 aatgcacctg aaatagcatt tcctgcaatc aatccaataa ctggatgtcc agcttgacgg
    79861 agttttgcat aagcagcagc actattagcc aaagccacat ggataccgat caattcttcg
    79921 ttatagccgt atgcttggct tggtacatcg atcacaacga tgattggccg tttcactttt
    79981 ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc ccatgcggaa accttcaact
    80041 aaaccaactt caccgtttcg tacacggtac atcgggtttt taggatcagg tacgattgat
    80101 gtcacgatgg cttcttctat gaatttactt gatttaccat aatacacagt actaatagaa
    80161 gaagttggat ttttcatgcc agttagtgcc gcaaaccaat cgtaaccaac acttgctgca
    80221 gctggttcat ttccttctgc tacttctggt aagtcaattg attttgcttg atggttagca
    80281 attgcttcat tgtaatcgtc aatggccatt ggttttgaaa gatctaaaga atcaagtaat
    80341 gataaataaa atgctgagct ttcactgcga tgcgtgtctt ttcgttcgtc cattgctgcg
    80401 atgacagcag ctttgattgc ttctacacta tcagtcacta attcatcaat gatttttgat
    80461 tctacccgtt gacgagatcc taatgtattc caaatcaagt ctttgtcact agaatcaaat
    80521 tctcgaacgc ctgcttcttg ttcaataact tctggtccat tcaaaccgac tctcgctttt
    80581 tttgttgcga ttaaataact cattaatgct gaagtaatcg acataccgcc aaatgatcct
    80641 acacgtcctg gaatcaaacc gatgactggt acatattgtt ttagagcaat gattacattt
    80701 ccgatttctg aaatagataa cagtccgtag tttgcttctt gcaaacgaac accacctgta
    80761 tctagtacaa tgactggaaa gatctcattt ccttttttat tttcttctaa cacatgttca
    80821 agtgcagcaa cgatttttgc accggaaact tcaccgatac cgccaccttg gaattttcct
    80881 tccatagcga ttactactgc ttgtttgcca ttgatcgtgc cacggctgac aacgatcccg
    80941 tcatcacttt cagggacgat tccttgtgct ggtaaacttg gcgcgatgat ttgatcgaat
    81001 ggatcaatca attcacgtcc attgtcaagt agagcgttca ctcgttctct ggcgtttaat
    81061 tctacaaaac tatttttcat ctgaaagttc ctccattgct tgcgtcaatc gtaggtacac
    81121 aactcctggt gtagcaccga agtcattgat cacgatatct gctgaaattg gataacgatc
    81181 aaaaaatctc gttaatacat tgttccatac ttcgccgaag ccatcgcttc ctgtacaaat
    81241 ttttacagta gattctgttt tttctgttgg aaacatcaag atttccaaat ctcctgaacc
    81301 gaccactcct acatggattt tcttattgat cggctgtttc gttggatatt tataagctaa
    81361 ttgttccatg gatctctccc ctttttgtaa aacagagcat acacgttctg cttattcgtc
    81421 tttcttcttc catctgggta aatggcgaca aaaggttttt ctcgccaatt ttatgaccaa
    81481 gttctgaatt tcattggtgg ttcatacagg ccatctgacc attcgatcaa atcatcgatg
    81541 gtttgtgcag ctaataatga acgtttcgct tcactacgtt tgatgcctag atcttctggt
    81601 aatttcacga tgccggctgc acgcaagcta tctagtgatt ctttcgtgct agtcattcca
    81661 agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc gttcttcttt tgtttttgct
    81721 ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg tagtatcttc gctataaatc
    81781 atcactggtg cattggctaa gcctgcttgt ttttgaacag ctagtgcatc taattcttcg
    81841 acaaagactg gttttttatt tgaaccatat gtttcaacca tttgaacaac taatttttgg
    81901 cctttcccta atggatcatc attgtcgcga agtgattgcc atgctggtgt agaatgacga
    81961 cgaccgccag gattatgtcc catgttaggc gcaccgccaa aaccagataa acgtcctttt
    82021 gttacggtag aagagtttcc ttcgtaatct aattgcaagg ttgaaccgat aaacatatcg
    82081 atggcatatt gtcctgcagc ttgtgataat gttcggttac tgcgcatcgt gccgtcttta
    82141 ccgacaaaaa agacatctgg gcgagcagca atatattttt ccataccaac ttcgccacca
    82201 aaactatgga tgctttcaac ccaacctgat tcgatggctg gaatcatcgt tggatgtggg
    82261 ttcaataccc agttcttcgc gattttccct tttaacccta gtgattcacc ataagttggc
    82321 agtaacaatt cgatagctgc tgtattgaat ccaataccgt gattcaaaga ttgtacgccg
    82381 tgttctgcgt agattccttt gattgccatc attgccatta gaatttgcaa ttctgtgatg
    82441 ttttgaggat ctcgtgtgaa taaagcttct aattgataag gtttatctgc tttcacgaca
    82501 acgtctaccc agtcaccagg gatatctaca cgtggtaaag aatcgacaac ttcgttgact
    82561 tgaacgatca cgataccatc tttgaaagct gcagcttcga tgattgttgg tgtttcttcc
    82621 gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt tatctgcagc tactaaaaca
    82681 acatttggaa tcaaatcgat gaataagcgt ccataaagtt ccaaataagt atggatatca
    82741 cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac gcaagctttg tgcaccagca
    82801 taagagaaat cgatcttgct tgcgattcct ttttcaaaaa tattcaaatg ttctggtcgt
    82861 gagatactag acatgatcaa atgcagatca ttcacttttt ctgcatctac tttttcaaga
    82921 ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt ctaatacaac tttgtcgccg
    82981 ggagcaatca gtttttccat tacttctacg attttttcag tttctacata cttgccattg
    83041 agcaagttgc tgattttttc caatcgctgc gctttttttt cgcgtttaga agcccagttg
    83101 cggtttggtt tttccataat tagtcatctc catttttgat atagaaacgt ggcgtcatca
    83161 ataccatgtt ggtctagatt gatcgtacgg atccctttat ctacaccaaa ttttgtatga
    83221 atcagctttg tcaaagtatt gccgggggga aattcaatcg tcagatccat tcctaattct
    83281 tttgccaagt ccatcatttg gttccactgc acagggaaag ctaggttttc caacaaatct
    83341 ttacgaatct cttctgcgtc ctgagtggct cttccatccg tattttttat atacaaacat
    83401 ctagcatcag atagatggac agactgcata tacggcttga atttcttgac cgtatcattc
    83461 atcaagagac agtgagatgg cacagggact ttcagcagct tcgctgtttg cgcttggtgc
    83521 cgtttggctt ctgccaacgt aacggataaa ccttcgatac tcccagataa cgtatgttgc
    83581 atcggacaat tttcatttga cagataaact ggatggtcag gatcaaatgt ttttgctaca
    83641 atttcctcag cttcgcttct cgtcacacct actactacgc ccattgcata accgcttgga
    83701 taagcttctt tcattaagac tgcgcggtga tagactagtt ccgctgcatt ctcaaatctc
    83761 aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg aatgtcctgc tgcaatatca
    83821 ggctgaatac cgtttttttc catttcatct aaataaaata ctgcttttgt cagtaatgct
    83881 agctggataa aaaccgtatc ttgatagttt tcttctttat caactaaccg ataccccgtc
    83941 acattttcta aacgttccat gtacatttca gggatatccg ctaacatccc taacttctga
    84001 ctgccttgtc caggaaataa gaaacctgta ctcatgagcc tcgcctcctc agcacttact
    84061 ttttccactg ctatcttagt caatttctgc tataataaaa aatgccaaaa aaagaaaaaa
    84121 ccataacaaa aaggtatgga tgggatggat aattatgaaa ttgcttcatc tgcagtattt
    84181 taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc aaagaattat ttatttctca
    84241 acctgccttg agccgtgcga tcaaacattt ggaaaacgaa ttaggtgtcc ctttgtttta
    84301 ccatagcggc agaaacattg agacgaccgt ttatgcagaa gaattttatc catatgcagt
    84361 caagacatta gacacattag aagaaggaat caaattattg tcttcggtca atgagaagat
    84421 cgttacttcc gtcatattgt atttagaggt agcctctgtt tctattccga acttggtccg
    84481 aatttttatg gaaaagcatc ctgatatcca attaacgatc atgcaacatc atttaccaga
    84541 tgatattacc gatcctgtac tgtacatcac ttcggagatg aagcctggca tgaccaatat
    84601 tcctatcatg aaagaacctg tttatgtcgc cattcctaag aaacatcctc ttgctaaaaa
    84661 agaacatctg caattagaag atatccaacg cacccccatc ttgatgctgt caaagagcaa
    84721 cgccttccgt catacactgg atgcagcact tgaagcgaaa agtatcgaat tgacgatcag
    84781 ttcgacgaca gatgatcctg cgacattacg ttctattgtc aagcaaggat tagctgttag
    84841 ctttttccca aaagtttctt ggtcttacga taaaaatgat cctttcgtct tgcgggaaat
    84901 caaagatttg cccttgacac gaacaattta tttatcttct agcttcacag aagatcatcc
    84961 tttgatcaaa atgattgcaa aaacgatgcg ggaattttat ttaggaaaat aaaaaaaaaa
    85021 gagctgggac aaaattcttg tcgcagctct tttttcgaac aaacggtgtt ccagaagtaa
    85081 tttcttagaa aataagccga aatttcccaa aaatttgaga agcaattttc gtaaattcct
    85141 tcttatttct tgaagctaaa catttctgtc acaacctctt ctctattgtc ctagacattc
    85201 attgcgttga tttaaaactt ccatcaatca aatctgctag ttcatctagc ttttctattt
    85261 tacgtagatc aatagagatc caatgttctt tattcatgtg ataggctgga aaatacccct
    85321 cttttcctcg caaaattccg ccaagttcag gatcgacctt caaatcaaga atattgatga
    85381 tcctatggtc acttgcatca tcatagattt tttcttctgg aacagacatc aggaccccaa
    85441 accatttttt gcttttttga tgtctgaata cgcaataatc aggaaatttt ttaaaaatat
    85501 attctggaga caaattgtat gtttcattga tgtgatcgat tagttctttt ttcgttagca
    85561 tctgcttcca tctctttctt attgattcgc tttattcaaa gataccacgt aagaatagaa
    85621 aaatgaatac aagtagttgc taatcaaata cttcgatatt tttctttttt atcaactctt
    85681 aaatgttcaa tccgcctgtg ttggtcttgc taccatctac tattaaaatg tttattattg
    85741 tttgcccacc tattttttga ttttagtggt atcatttttc aagttcatga tgaaattctt
    85801 atcatcagta aatgctccag aaaaataaac aatgattgaa aaatcagttg aaacttgacc
    85861 accgtttatt ttctccgtat tcttagaata gaagggagtc tgattgatgt attcactaat
    85921 gaaaaaaatc attaccgaaa aagatattcg acgtcatgtt tctttagtag aacaattgct
    85981 gaatcataca aagataaccg taaatgaatt agctgaaatc atagggacaa ccgaacgaac
    86041 gattttttct gaccttcaat ctattcgctc tcagctgccc gaaggctggg acatcatcag
    86101 cgatcaagca ggaatcagtt tgcaaaacca gcaaaattta ttaacaaatg acctttggga
    86161 gatttttttc aaacaatcag tcagtgtcga actgctgaaa aatctgcttt ttacaaaaaa
    86221 agttgctgtc cctgattttc tagccgacca tgggctttct tatgggacat tgaaaagaca
    86281 tgtgactaaa atcaatcagc gtttagcaag ttacgacttg cagatcgatt tgacgaaata
    86341 tacagcttgt attcttggaa aagagcgtgc gattcgtact ttttatcatc gattgctgat
    86401 tcctttcact cataacaact attttttcga ggattattcg atccatgaat cccattattt
    86461 tcaattttta agaaacctca gtcaaactga actagcggtt gaaacagaag agattttcgg
    86521 aacatgttgg ttctttatca atacgatccg tataaaagcg aattgccgat tagattcttc
    86581 tatccatacc aacagcacac tctcctccct gtatgactct gcattgaaaa aattgtattt
    86641 aacggaaggt atttatctta aagacactga gctttctttt gcttctttct gctttctgga
    86701 aagttggaat tacaacaata actatggaca agaaaccgct cgctgcctgc atcattctcc
    86761 atttttagag gtcctcgaaa cgtttgtaga ggaactagct tcagagctgt ctcttgatca
    86821 actaaaaaaa acatctctga ctgataactt ggctctcctc atattaaaat atcatgaatc
    86881 gccgatattg attgaagcat tggaccgtca ataccatcgt tttttagaaa catatgatca
    86941 gcaatatgca cacttgtatc aacagaaaga gaatttactg aacgatctaa aaaaagaaat
    87001 agaaatcaat gaaccaaatt actttctcca actcttgtct ctactgatcc aacaaacgat
    87061 tttttctatc aaaccgaatt tgttcaatgt ctattttatt ttccaaggag aaccttcatg
    87121 gaaggcgttc ttgcagcagg aattaaaaga ttacttagga aaacgtgtct gcttcttacc
    87181 tatcgaacta accgatcttt ccactatttc ttttgaaaaa acagatatct tgatttccaa
    87241 tttccctcta gattctctag atatccctat tgtatacatt tcttctattc caacgaaaaa
    87301 tgagctcaat cgtttgactg aactcacttt caaaactttt ttatagtaac ggctagcgac
    87361 tgtattctct agctgttttt tcttttttgc gaaacagttt gttcatgatc cctttttcta
    87421 tttctacaac taagaataga agaatcacat ttgctccaat caataactgt tgtgtcatcg
    87481 ttaaacttac tgtaccgatc agctgctggc cgactggtag aaaaataacc atcccttgca
    87541 ataaagcaag gatacctaat gaaagaaaaa gcgctttgtt ttgcaaaaga ccttggttca
    87601 atgacggctt ggacaactct ctgcaattga tcatgtaaac agcttgagct aagacgatat
    87661 tttgcagaag aatggtttgc tgtaagcttt gcccttcaaa ctgcatagcc aataaatagg
    87721 caggaaccat gatcagtagg gagacataga tgatccgaaa caaatgataa ccagatagga
    87781 ttccttgctt tggatctctt ggtgggcgct tcatgatttc tggatctggc gactcgaacc
    87841 ctagtgcata agataaagtg attgtcgtta ccatattgac ccataaaatc tgtactgggg
    87901 acaaaggtag cgggcgattc agcataagtg cccaaatcaa gatcaatcct tgagctaatg
    87961 cagttggcaa gaagaaattg atggtttttt gcaaattatc caaaatccgc cggccctctt
    88021 ttacggcttt tgcaatcgta tggaaattat catctcctaa aaccatatca gctgcctgtt
    88081 tgcttacttc actacctttg attcccatag caattcctac atcggatctt ttcaaagcag
    88141 gtgcatcatt tacgccgtct ccggtcattc cgacgatttc tccatttttc tgcaaagctt
    88201 taacaattcg cagtttatgt tctggtgtcg ttcgagcaaa gacatcgact attggcactt
    88261 gctgaatcaa ctcttcatca gacataagat ccaaatcgat cccttctaaa actttggcag
    88321 tatgttttaa gccaatttgt tccgcaatcg cttttgctgt gtctttgtga tcccctgtaa
    88381 tcattttcac tgagatccct gcttgttggc attcctttac agctttgact gcactttctt
    88441 ttggcggatc gatcaatccg gcgatccctg caaaagttaa ttgggttaaa gtctcatgcg
    88501 tgagttcctg tttcgaatct acttttttat aagcaaatcc taaaacacgc tgaccttttc
    88561 tcgcaaattc tgcagctgtc tgactccatg cttcttgttc tgcagaagaa agagttgatt
    88621 tagcaaataa tacttctggt gcacctttga cgtaaatcac cgctcctttt tcctccgcat
    88681 gcatcgttgc catatatttg tattcagaac tgaacggaat ctttgcttct atttttttca
    88741 aaggtagatt ggctgtttct gtgtgtcgaa tcagcgcaac ttctgtcgga ttgccactta
    88801 aattttctgt tttctgttct tcttttgttt ttaattcctg acagtttttc ataatggaca
    88861 acacgcaggc ttcttctttt gcagctacat ccacaactgt catttcattt tttgtcaatg
    88921 ttcctgtctt atctgaacag atcactgtca tagaacctaa tgtttctacc gaaggaagtc
    88981 ctttgatgat cgccttttct tttgccattt catgtactcc aacggataga atcatggtca
    89041 aaacagctgg caatccttcg ggtaccattg acaccaccaa agcaatcgta gccgaaagaa
    89101 ggatatgcca atccatacca tatcgaaatg tcgtaaaaat aactaagaat aggctcaaac
    89161 aaagaatgcc ttgaaaaatt tgtttgttta atcggttgat ttttttaatc agaggcgtcg
    89221 tttgttgttc gaccgattgc aatgcttgat tgattttacc gatttctgtc gtgtttcctg
    89281 tttctacaac gacaccgact gcagaaccag attgtacaag cgttcctgaa aaagccatat
    89341 ttttctggtc gccgatcgat tcttcttctg tcaaaacggc atgacttttt tcagatgctt
    89401 ctgattctcc cgtcaaaatc gattcttcca ctaataaatc atgtgcttcg attaatcgga
    89461 tatctgctgg aacgacatct ccggcacgta agacaatgac atctcctttt accagttctt
    89521 tcgcagggat tttttcttgc atcccgtttg atagtactat cgcttcttgt cccatcagct
    89581 gcttcaaacc attcaatgat tcttccgctt ttctttcttg ccagtaacta acgagtccat
    89641 tgacgatcac tacgaataaa ataatacttc cttcaatata ttcactagaa gcaaatttca
    89701 aaaaagaggc aatgatcaac acgatcatca gcagatcagt aaaatgcttt gctatttttt
    89761 tccatcgttt tgtctgctgc cttgctttta tttcattata tccttgatca gtcaggcgtt
    89821 tctgtcgttc ttcttgcgac aagccttctg ttgatgtttg atagtttttc aatacatctt
    89881 ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt ttaattgata ctgttattat
    89941 acgcagggaa gttttttcag gcgtatcgat tatttcaggg gggtctgaaa taattcgttt
    90001 tgcaaaatgt atctgggcct aactgatatt tctattgctt attcttttat atacaaaatt
    90061 taagaataca aaccagataa ccaataaaaa aggaataaag cgtgtcgtgc tttcgaataa
    90121 caagctgaaa gcaattgaag taaaaaacac caacaaagca agatctttat atctttgtgt
    90181 agtgttccgt ttcttttctt taataaattg aacatgtgca cacaagatcg aagaccacac
    90241 aaatagaaaa caactcgttg tcatgcctgc caaaagctga aaaaacaatc gactatcagc
    90301 tacaaaaaaa tgtagacaaa gaccggcaaa aaataacatt cctgtaatta aaataccgtg
    90361 taaaggtacg cctttcttag aaagcgcaca aaaccaatgg ggcaaacttt tttcttttgc
    90421 tgtttgatat aaaatcctgc tagtactgta gactgcacta tttgctgagg acaaagaagc
    90481 aaccaaaatc attagattga caattgctgc tccgccttgg caatggatca cttgcagcat
    90541 agtcactaaa ggactttctt ttaaatctaa acaatgccat ggaacaacaa ataaaatcga
    90601 caaaatcgtt cctatataga aaaagagaat acgccaaggc agacagttga ttgctttttt
    90661 caaattcttt tgcggatcac ttgtctcgcc aacggttaag ccgatcattt caattccagc
    90721 aaaactgaat aaaaccatag gaaatgagac taccattcca gcaaagccaa acggtagtaa
    90781 ttcatcgatc tgtccaaaat tttctttgat tccgatagac tgggtatgaa aatctgagac
    90841 actcaatcct atacccaata agataaaaag gaaaatcccc aagatcttga tgattgagaa
    90901 aaaagattcc aattcaccaa accacttgac tgcaacaagg ttgactgcca ttaagatcat
    90961 tagtgcgcta cctccgctta tcaaagctgg tacatgtaca aaccagaaat ggatatacaa
    91021 tcctattgct gttaattccg caagtccagc tccaagccag caaagccaat aagtccaatt
    91081 gacaaaaaat gcccactttt tgcctaaata atagtcaacg aaatcactga tagagttaaa
    91141 tgtactattt gctaaaaata aatcacccat ggcccgcatc aaaccataaa tagccaaacc
    91201 gcatatcaaa taaacgagaa taacagctgg ccctgctgat gcaattgcct ttgctgaacc
    91261 taaaaataat cctgttccaa ttgcgccacc taatgcgatt aactggatat ttcgattagt
    91321 tagagtccgt ttcattgccg gtttttccat caagttcacc tttttctttt tcaagcagca
    91381 tggcgacttc ctgttcccaa gaaggtttct ttattttttc aatacagagt ggtactacaa
    91441 acaaaatgac actgatggct gtgactgcaa ttagattgat ccatgttcca gctttcatcg
    91501 tcagtccgac agaaaatccg attgccgcaa ataaaacaat aacgacagtc caaactccaa
    91561 gattgctctt tttctctttt tttccaattc taaatggtcg attcaacttt gcatgtgtat
    91621 aacgcaatct caaaactgcg atccccatga tgacatagac gatacagtag ataacagtag
    91681 ttgaatttac gagcatcaaa aatgcttgat tgacgccagg aatcagcaaa taaaaaagtg
    91741 caaagattga gataatgaca gactgagtca aaataacgga tttggatacg tcaaattgat
    91801 tcgtcttcca aaaattgaat tttggtggat acgcaccttt tctagcactt tctgtcactg
    91861 tctttgctgg tcctgaggcc caagcagata attggactgc tacaccaatg aagacaagta
    91921 aactaaacag attcacaata agatgtggca aaccaagaat ttggcaataa atcgaaatag
    91981 attgcgcgat attattcaat tccatttgtc cttttggtac gacatttgct acaactaaag
    92041 cattcaaaat attgacgaaa aatataaaaa ttaacgcggc aaatactccg cgaatatacg
    92101 ttttaactgg attttccaat cgagtgatat acacagagga catttcgata ccagtaaaaa
    92161 taaacatgat cggcgcaaaa taaacaaatg aggatgcagt cgtagtatct ggtattaatt
    92221 tgtcccatga aaaactgcct aatgttccgg ttggtacgat cccaactttg atccacgcag
    92281 caaaccctaa taagagcatc atcgtcaaag gcacgtacaa tcctaaccaa acaccgactt
    92341 ttccgccgat ttttgccata tcaaatttca aattcaaaag agtgatgatc caataaacga
    92401 ctaaaatgac aactaatgta aatttgctgt tcaaaccaag cggcacatta tcgataatat
    92461 taccaaacaa cggagcgagt gctgaagcga ccatcaccat cccaggaaac atttggaccc
    92521 ataacagcca tgagacaacg aacccccatt tttttcctag agcattcgat acccaaagtt
    92581 ctggaccacc tttttgggga aacattccag caaattcacc agagatcaat gagattggaa
    92641 aagcgtacaa taatactgca accagcatgt aaaaaatcat cgtccagctc gttgctgcta
    92701 catctggaat atttcgtaca cttgctacaa gggcgcaggt cattccgata aaggtcatgg
    92761 tcgtgatttg ctttgatgaa ccagtatctt tcatctttat tccttctttc aatagcgaca
    92821 tatgtttttg ttttctttta tttttgttca agaacagaaa tgacaaggtt tcatcgattc
    92881 agtacaaaac ccagtatcac cgaaacatcc attgaacgat cttagtgaac tgggtttgat
    92941 acatgaaacg aacgatccaa cttatagaac gttttataaa aaagaatatc ttggcgagga
    93001 atatatgaaa aacaggggga aatacaattg ttttgatgaa gcaaataatt gtctgttctc
    93061 catatgtctc ttgttattct ttcacacaaa aatcagttct attggatgat ttcgtttagt
    93121 ttcttttcca ttgctttggc gatttttttt gtataaaaat tgaaaattcg atcatcattc
    93181 aaataaggag tcatgatagc cgcacgtagc agagtgattt tctgttcttt tttccattct
    93241 tctttcgtta gacccattct ttcaacaaaa cgaatcggag aatcaccgta atcttcttgt
    93301 gtaaatgtag tatgagaagt aataaaccgt tcaccataaa catcgccatc catataagaa
    93361 gaccgatcga acattttttc atttaattca ttgatagcgt tcaaatctgt acagccttgt
    93421 tctttgaata cccaattgac catattaaaa tcaggatgat tcaatggata gacttcgatt
    93481 gtttttcctt taacagtaaa agtcagatga tcgagaaatt ctctgaatct ttgagctgcc
    93541 tcgattgaag ccccaatcaa ttgaccatat ccggtcacat tcaatggcaa tacacgatgt
    93601 gctgtccaga ctgcggcggc ggttgctcca gcttttgatc cttccaaaat ataagctcct
    93661 aacatatctg gagcctttac tgatttttca aacacatatg gcgcgaaata agaaatgata
    93721 tttctcatat ttttatgttt gattacgatt cctccagcag cataagggac atagcccatc
    93781 ttatgaggat ctatcgtgac agaatccgct tctgaaatag ctctgaaccc ttcatatacc
    93841 tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt cttcaaagaa ttcagctaag
    93901 gaagcatacg gcacaaattc tcctgcttcg tttagaaaca gtgaacgcgc atagccgcca
    93961 tatgcggcat caacatgtaa atagaaatag atcccttcat cttttaacct ttctcttaat
    94021 tggacgattt tatctacact gtctacttga ccttcttctg tcgttcctac gacagcaacc
    94081 acacctagaa ttgggatttt ttgtccagct aattcacgaa ttgttttttc caaagcatta
    94141 atatccattc gataatcctc ttgaactggg atagcaacca tctgatctaa gccgactccg
    94201 caaatatcta acgccttcat ccaagaatag tgttttgttt gagggaccag ccatttccct
    94261 aatctttgga tattttttcc gctacgggaa gaagctgctt tgacctcatc catttcttcg
    94321 tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag ataaattcaa aagttcccac
    94381 tcagacattt ttttcacttt ttcaggcagc acttccttga cagctaaagg aatcgatttg
    94441 atgcaacggg cgtaccataa tccttcaaga ttagcaatag aaccatctgc tgcaatgtgt
    94501 ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct gtcctacttc tgcttccatt
    94561 tgcgaagttg ccatagaaga ttccaatgca acattatttg ggttccacag catcgcatag
    94621 ttgtaagcta ataaagcagg catcaatgtt tcagcattca tttgtcccca gtaacggcct
    94681 gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac tgacttcatc caagacctct
    94741 atcatatggt cgcgggtggc tagataatct ggagtgagct tatctccttc gctaattgca
    94801 ggcatatctg aaggaatata attttctctc catccaagat gttcatctac catcttgttc
    94861 aacaacattt tataaactgg tccattttca gctttgtcgc ctataaagac ggccttgata
    94921 tccatatctt tcatttttct tccctccata ttttgttggt caatgtcttg cagatacatg
    94981 tgtttatctg taacacctct tcactatagc gccaaaaatg agaaattttt ttttataaca
    95041 tcaaatccac gtagattttt acaaacaaaa ataaataaag tcggattttt ctgataattc
    95101 aacgcaaaaa agcgaacaat ttttcaataa atcagtcttc taaaatagat aacctaaaaa
    95161 aagcgaacaa acaaataaaa tgactgtcct tcattcacta aacatatttt tattttttta
    95221 tattgttaaa aattcacaat ttatttttta atagaattat ctgttttcca aattttataa
    95281 cataaaaaac cttttttaac ggcatttata tgctttttca gaaattaact gatgatttca
    95341 aaaagaaaac atgtcataat atttcccata ttgtgcaaaa aaatacaagg cggaggctct
    95401 actatgattg aagaatatat tgagaaaaat attttaagac agctgttttt atgtgggcaa
    95461 ttctatgtaa ataaagaagt caatctagaa aagctctcca atctcctgca tgtttgcaaa
    95521 accactttac tcaatgatat caataacata aaaaaagaat ttgaagaaca gatcgcttat
    95581 acacatcggg aaaaggaccg ttacacttta tatttttcag aacatatacc tcgatgcaag
    95641 atcatgcagc aattgtctca aaattcgttg tttttaaaaa cttgtctgct ttatttggaa
    95701 gaagatgaac ctgattatct tcaattaact gaatgtgaat tcatctctgt cagcaaagct
    95761 tattcattaa aaaaacaagt attagcttac tttaatgact gcgggatcga aatcgaccgc
    95821 tactcccctc gattcactga aatggaaaga cgtctgctgc ttcttaatgt gtcgtatcgt
    95881 ttaggcggtt ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc cgatcgtttc
    95941 attgaatccg taacggaaaa tagtggcaga ttttatgata aagaaaataa agaaatctta
    96001 tcgattggtt ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac aatagattca
    96061 aaatttatcg aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc agcatgggaa
    96121 aacactgatt tccaaaccta ttataaaaaa gaagaattcg cctttatttt gacactattc
    96181 aatctttgta attatggttt ccattcttat cagttgattg cggaagactt tcaacagcta
    96241 caccaagtgt ttattgacaa tactcccgaa ataaaagaac tagttgctac ctttgaatct
    96301 cacttcaatc aagaattatt cggaaaccaa ccgttcgaac gtgcgctgat tcatctgatg
    96361 cgttcggctt gggataacta tcaattattc atgccagaaa aattttatct tttaaatgag
    96421 gaacagacaa atttatataa agaagtccag acaatttttt ccagctggtc cagccagtta
    96481 ccttatgatc tacggttgaa tcctaattgc atgcgggctt ttgtgatcga attgagcgga
    96541 attctccgtc tgacaaaaga acatctgact atctatatcg tcacaaattc ggatgttcat
    96601 tatctgattt atcgggaagc cttggaagct gtaactactt tcgattttca agttgcacca
    96661 acaatttatt caagtatatc tgatataaag aagtatgcgc agcagtcctc aaaccgggta
    96721 ttatgtgaac gtacattata tacaccagat gcggtccaat atgaaaatat tattcctatt
    96781 tcgatcaata cgatcgatcg agctatcatt tcagctgtgc aaaacaaata aggggctgtg
    96841 acaaaaatca tgtcacagcc tcttatttgt tgaagatgcc catttctgta acaatctctt
    96901 gatacacgtc aggctcacaa cctcacttat tttggacatc taggggtccg atctctttat
    96961 ttaaaaaata agaaatgatg gttcggataa ctaccaacgc cgctagttgg aaaatatcct
    97021 gcaaggtagg tttgataatc gattcgatga tcccagctga aacaagaatt tccaaactta
    97081 gaagtatata gcctcccaac atctttttaa tagcattgtt ctcgcttact cggtcgcttt
    97141 gcacttttgt atggacaaat aaatatttca aaaaatcctt gattgataga caaacacccc
    97201 aaaccaataa acagatagca aagatatcaa gtcccagtac aagtttttcg aaatatggaa
    97261 gaagaaactg catcaactct gtcgtcatcc tacctctcct cttctttgtt tttctttgtc
    97321 ttctatttcc tgattcaaga aataagaaat gactgttcga atgataataa taagtgcaag
    97381 cttcaaaata tctgtccaag tcggcttgat aactgactcg atgatatcag caacgactag
    97441 tacttccaag cttaacaaaa tataacttgc tagcagtttt cttgtatctg cattacgagt
    97501 attacgttct gcatagtttt tcttgacatg agaccaatca aaaagtttga atagaacaag
    97561 aacgatcccg acaattaaga taccgataga acaaatattt aaaatagcaa tgatccattc
    97621 tagaaattca attaaatttg agcgaaacat atacgctacc tactttcttt taatttgttc
    97681 ctattctttt attgtaaaag aaagttacct tgttcgtcta tttaaaagtt gttctaatga
    97741 taacggttcg cttaaatttt tctttttcac tctcccattt ttctccctcc atccataaat
    97801 cgtggaagta tccaaaacaa cgtagcaagt taaaataagg cttagtccgt catcagcttt
    97861 tcaaagaagc actgtctcgg tgcttttttt gtacccaaat ttctaaaaat agaaccatac
    97921 acctcctcaa atcttttctt cttacctacc ctatttttat ttacaggaaa atcagacact
    97981 tcttcttttt tgtcaaaatt tcctttttct tctcgtccta tcatttggca ttctaataaa
    98041 aacgaactat ccttggatat agaaagagaa aacaaggagg cgtttttcat gagtaaaaaa
    98101 ttaatcaaag tcggcatcgg tttaggtttg cttgcccttg gcgctgcata tctaggaaaa
    98161 aagacaggac tattcgaaga tgacagtcat ttatatgatg aattcgaatc aatctagcat
    98221 cctatttagt tgaatcacta cggacatact tggaagacat tggaggaatc acaatgagaa
    98281 aaagtaaagc aataatgatt ggtgccggta ttgccaatat ggcagcggct gtttatttga
    98341 ttcaagaagg caaatggaaa ggggatcaga tcacctttta ttcattggat gatcacggtt
    98401 caaatgatgg tgcaccgacc gacactgtag tggatgaata ttggaataaa aaccatccat
    98461 tagaaaacca aaaaggctat attgctcgag gcggtcgcat gcttaattac cgtacctatg
    98521 tagatttgat ggatctgtta agtcgaatac cttctgttac tgagccaggg atgactgctg
    98581 aagaagatac gagagatttt gatgcaaaac accgaacctt tgacaaagca cgtttgatgg
    98641 aaggtggcaa aggaatcatt gatggcggaa aactgggatt caataataaa gaccgtattc
    98701 ttctgactaa actgattgct atgcctgatt cagaagaaga aaaactagac aatatcacta
    98761 tcgaagaata tttcaaagat gatccacact tttttgaaac aaacttctgg tttatgtggg
    98821 aaacaacatt tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc tatatgcatc
    98881 aaatgattta tgaattcact caaatcgaac atctagtggg cgtcaatcga acacgttaca
    98941 atcaatacga aagtattatg cttcctttga tcgagtactt gaaagaacaa ggatgcaaaa
    99001 tcattttgaa ccgccgtgtc actgactggg aattcaaaga aacaccgatg caagatgaaa
    99061 tcactgtgac tggtttgaaa attaccaata cactgactca agaagaagaa tacgttgtgg
    99121 tcgatgaaga tacagcagtt attttcacta atgggtccat tacagattca gctactttag
    99181 gagattacga aacaccagct gtagaaaata tggattatgg tgcagcttct agtctctgga
    99241 aaaaagccag tgaacgtttc tacaacttgg gaaatcctga caaattcttt gctgatcgag
    99301 atgcaagcga atgggtcagc tttaccctga cgacgaaaaa ccatttgttg ttgaatgaaa
    99361 tcacccgtat cacgactcaa gaaccaggaa atgccttgaa ttcatttatt tcaaccacac
    99421 cgatcacgcc gttaggccaa aaagatgtca atatgtctat tgtcgtgcat catcaaccgc
    99481 attttacgtc tcagaaacca aatgaaacag ttatttgggg ttatttcctt tatccaagac
    99541 gacgtggcga atttgtggat aaacagtata ttaaaatgaa tggaaaagaa atgctagaag
    99601 aactgattgg acaactttca aaagtagatc ctggaccagt gaatattcgt gaaaaagaaa
    99661 cagaaatttt cgacagcgta ataaataata tccctgtcta tatgccttat gcttccgcgt
    99721 tgttcaataa tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat tcgaccaatc
    99781 tggcctttac aggagaattt gtcgagcagc cttatcagat gatctttact gaacaaagtg
    99841 ctgtacgttc tggagaaatc gctgctttcc attttgcggg tattcctatg tcccgtcttg
    99901 tcaaaacacc gcgttacgac aaagatattc ctactctagc tcgtgccgca aaaaaaatgt
    99961 ttgaataatc aagaggttgt gaaaaagctg ccctgcatca agtaataaga acaaccaggc
   100021 taaaacagct tcttatgttt ttgatttggt tggacttatt accgcagatg caagcttttg
   100081 aacaccgttt accaagaggt tatgaaaaag ctgccctgca tcaagtaata agagcagcca
   100141 agctaaaaca gcttcttatg tttttgactt ggttagactt attaccgcag atgcaagctt
   100201 ttgaacaccg tttaccaaga ggttatgaaa aaggcgttta gtttcgagta tgaagaaaaa
   100261 ttttgtgaaa atggttcctc acgtttttgc ataaatttga cttaataccg agaaactgat
   100321 cccttctgtc ataacctctt tttatttatg tctgtttctt tttgaaaaca actatttttt
   100381 caacgtttac tctcttgcca atccaacatc atctaacgga tacaactatt tcaatgatta
   100441 ccattacact aaaaaatcgg aaacacacct ctccgaacac aaatacataa gactatagaa
   100501 tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc tttaaaatga
   100561 aattatctgt agtactaata atgcagacaa ttttatgaat ctgaaaggag catctccaat
   100621 gaaagcagtc acttggcaag gaaaacagaa aatggaaatc agaaacgtag atgatccaaa
   100681 aatattagaa ccaacagatg caatcatccg tatcacagca acagccattt gcggctccga
   100741 tctgcatctt taccatcatg gtgaagcagt tttggaaaaa gggtatgttg taggacacga
   100801 accaatggga atcgttgaag aagtgggatc tcaagttcaa acattgaaaa aagggcaacg
   100861 tgtggttatc ccttttaata ttagttgtgg tcattgtcat ttctgcctga atcacatgga
   100921 aagccaatgt gatgaatcaa atccagatca gttatatggc gggcttttcg gatttggcaa
   100981 gttgaacggt aatcattggg gtggtcaagc ggaatatcta agagttcctt atgcagactc
   101041 tacttcattt gttgtaccag ataatgatct tcctgatgaa aaagttttat ttctatcaga
   101101 tattttgccc acagcttatt ggagcgttga aaacgcagga gtaaaaaaag gcgatacagt
   101161 tgtgattcta ggttctggtc cagtcggtct attcgctcaa aaattcgctg ttatggctgg
   101221 agcagagcgc gtaatcgcag tagatccagt acagcaccgt ctagataaag cggctaatta
   101281 caatggggtt gaaacttatt tcttagaaga cacggcacaa gctggaacag aattgtatga
   101341 actggctaaa ggtggcgcag atgtcattat cgattgtgta ggaatggacg gattagagcc
   101401 tgtcaaagaa aaagcgaaaa atttagtaag cttacaaagc ggcacaatct ctccattaca
   101461 gatggcatcg caagctgtca aaaaatttgg aacagtccaa gtaactggtg tttatatgac
   101521 tccagcttct tcttacccgc tgcaagaatt ttttatgcgg gatatcgctg tcaagcatgg
   101581 acaggcacca attatccatc taatgccaaa aatttacgca atgattgcag aaggactatt
   101641 cgatcctaca caaatcatta ctcattcgct gccactatca gaagcagcac aagcatatga
   101701 gatctttgac aaaaaagaag ataaaaatat aaaagttgtt ttgaaacctt gaattcagta
   101761 aaaaatacag gtgattttct ttatgcccaa aatcaaacga aatacccatg agatcgaaat
   101821 aggaaaagaa gaagatatcg aaatacgagg acgtcgcttc cgcttctatt tccaaaatcg
   101881 ttacacattg atctcactgg ctgtcgattt attaaccggg attttctata tttttggaag
   101941 ccttgcttcc ttaactaaaa tccccgatcg atatggcacc tatttttatt tggctggtgc
   102001 tattttcttg acgattcgtc ctattctcag aatcgtaagg aacattttta tttacgacga
   102061 aaaaaaagca atgaagagaa caagcaaaaa aaaagaggca tgagaatttt tcaaatctca
   102121 tgcctctttt ttcaccctat cgtatcactt tgatacagac gagcggtcgt cccactttct
   102181 tccttgacca ttgtcaaaaa tgtccactcg tatcgttcat aaaacgtggt atgatctgta
   102241 attaagtaaa gtttttcgta cccaagttga tgcatttctt ttctgatttc cgctaatatc
   102301 cgcttggcta tcccctgctt acgcttttcc ggttcaacaa acagggcaca aagatttggt
   102361 gtcagatcag ggcggttatg gaagtcgttt tcaatgacgc ccgctcctcc gacgatggtt
   102421 tctttttcat ctaagaccac ataccattga ggaatacttt ccttattagc gattccagta
   102481 cgaatacttt cctcatacac aacagcagga actccccatt ttgatgaaaa ccaattcgcg
   102541 ctttgtttta ctaattctgg gtgttctctt aatttgatcc agttatatgt aaccaatgtt
   102601 taaacctccg actaaaataa aacgtttaca aaaattatac aagtctttat gaaaattagc
   102661 aatgacaaca atagtgatgt aaaaaaaaaa acggaaaaca atcacttgtt ctccgttttt
   102721 tgattaatca agcatctaaa gcttcttttc ttctttcttg gatcgtagct accttgacga
   102781 acggtagata gatcaaaata gaaagaacca aattgacagc ggctagtaca ccacctgcaa
   102841 tactttttgt tgccaataca ccgccaataa ttggtggagt tacccatgga ggcataacgg
   102901 tagctgctgg cactaaacct gtagaagtcg caaagtaagc aacactaact aagaccattg
   102961 gtgtcaaaat aaatggaatg aacatgatcg ggttcaacac gataggtaac ccaaacatcg
   103021 ttggttcatt aatattgaag acacctggcg caatcgacaa gtttgttaca accatatatg
   103081 gtttgttttt acgtcctact aaataaatag ctaacagtaa acctaaagtt gcacctgttc
   103141 ctcctaagtt gacaaagctg tcaaagaaag gtttgttcac gataaacggt gcgctatgtc
   103201 cagctgcgat tgcattaacg tttgcttcaa tggctggcgc attgattgtt tgcatcaaag
   103261 gatcgaccat attcgctccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa
   103321 gcagtaaggc agatggataa gagtttgcaa gtcccataaa tggttcttgg accactgtat
   103381 agaaagaacc tacgatatca tcaacaccga atgcagcaaa aattgcagca actaaagcaa
   103441 acgcagagat agtgatcatt gctggtagta atgaagcaaa tgcttttgca actgccggtg
   103501 gcacaccatc aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt
   103561 ctgttgaaat aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg
   103621 ctccgcctaa agcgaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc
   103681 ggtagccgtt agctaaatga taagcaagca agaaagcaat caagacagaa agaatggcaa
   103741 atgtaccgtt ccaagcagca gccccgaatc ctttccatga ttcaccacca aaaattgagt
   103801 tcatcaattc ttggaacccg ggaatcggta agttattgat cattactgct aatgctccta
   103861 agatcattaa cggcatactc accataaatg agtcccgaat cgcaactaaa tgccgttgtg
   103921 caccaatttt tgatgctaca ggtataaaat gtttctccat aaatgcaaca aatttatcca
   103981 taaatcttcc ctcttttctt tactgtagat tacgcgttca atcgacgata cagattaatg
   104041 atctcttttg ctaaatctgt aaaagctata gatgtcatca gatgatcttg actgtggacc
   104101 gtcaaaagcg tgacttcaac gtgatttcct tgtgcttctt gtgtcagcat ctctgtttga
   104161 gagtgatgtg cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg
   104221 aaatcacctt ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca
   104281 aacatgatca gacccatgat ggcttctaga tttttttgat cttccattta ttgtatcctc
   104341 cgataatttt tattatttgt ttattagttt ttctgcttgg tcgagtactt tttcaccatt
   104401 catcatgcca tagtcagcca tattgattac atctaaagga atgcctttag gtgctagctt
   104461 ttcagcaaat tgattttgca taaagcgtac ttgcggtcct aaaagtaaaa cgtctacatt
   104521 tttagattct agttgattat ccgcatctga agcagataca gcaaagatgt ctgcttccat
   104581 tccttttgct tctgctgcct tttgcatttt tgtgactagg agacttgtac tcattccagc
   104641 tgaacataca agcatgattg ttttcttttc cattttaaac acgttccttt ctatacttac
   104701 acttgtatat aagcaagtag cgtgccaact ttaaaagcgc tttaaaaagt tgatatcatc
   104761 acatttaagg tctataccaa gttgcataca cactaaaaaa gaaagcgcac actaaacaca
   104821 caaattaatt tttattacct tttttctttt aacgtgcaca aaaaaagttg tgacacaaaa
   104881 cttgtcacaa cttttttcaa ataaacggta ttcaaaagct tgcatctgcg gtaataagtc
   104941 caaccaagtt aaaaacataa gatgttgttt ttactttgct attcttatta cttgatgcag
   105001 gacagctttt tcacaacctc tcaataaacg gtgttcaatc agctgaccct gggtattagc
   105061 tcaaaacgct ggattcacaa cctcttttat ttcaaataac cagtaaatac aatgaacatc
   105121 tacaaaaaac ttctatttat ttatagattt cgtcaggcaa atccaattct ctcttatcaa
   105181 tcaaagtctg ataccacgaa gcagattttt tcaatgttct gcttcgttca tcgttcaatt
   105241 cgattcgaac taatccatat cgattcttaa atgcattcat tggtgacaca cagtcagtga
   105301 atgcccataa catatagcct tcgcaatttg cgccttcttc tgttgctttg atcaagtaat
   105361 gcaaatgctg tccgataaat tcgatacggt aatcatcttg cacgattcct ttctcatcca
   105421 tatactgttc ttcatattct cttcccatac cgttttctgt tatcagccaa gggatattat
   105481 gataattttc ttttagatac atggctatat catacatgat tttcggatag atttcccatc
   105541 ccctggaatt attcattttc ttcccaggta aatcaaagac ttcataatat cgttcaggat
   105601 ggaaaggtgt ctcttcattc cattgataac gaggggcgcg aacgcgttta ggataatatt
   105661 gattgattcc aaggaaatct actgtatgat gctgaatcgt ttctaaatcc tctgttgcag
   105721 gatcgaaata aatcgattgt ttttgacaca actccaggaa ctcttctgtg tagtatcctt
   105781 ttaccatcgg atcaaaaaat actcgattat aaaacaaatc atacatataa gcagcttttt
   105841 ggtcttctgg agaattggat cgagcataga ccatttcagg atttaaaata cagccaatcc
   105901 ttccaccata atttccgctg cggtaaatct ctactgcttt agccgttgct aaaactttat
   105961 gataattcca catcatccac ttttttgtgt tttgttcatg cggccagcga acagcatcca
   106021 aatagcaacg cgtttgggga acgattggtt cgttgaatgt aaaccagttt tttaccctat
   106081 caccgtaacg atcaaaggca atttgagcat acttagcata aaaatcaact acttttcttg
   106141 aagaccagcc atcatatttt tccaaaagaa aagctgggac ttcataatgt tccaaacaaa
   106201 tcatcggttc tacgtctgct tcgatcaaag agtcgatcat ttcacttata tgctgagcat
   106261 attcttcatc tactatacag ttctcataat cagtaaaaaa acgagcccaa ttgatagaag
   106321 tccgataatg tgtcagatgg atggatttca tcagatccac atcctgctgg tattgttcca
   106381 taaaattagt tgctacggct gggccatacc cattatgcca aacaaaatgt tcatttttat
   106441 accagctatc tataaacgaa tcctgtcctt ctttttttcc tttccaacct tccgtttgcc
   106501 aagcagaaga tgcagcacca agaataaaat tttttggaat agtaattttc atgatttcct
   106561 cctgtttttt agatcccatc attttccaca cgatttgcta cttttacaaa tggcagatag
   106621 atcaaaatag aaacaataat acaaacaagt tgcgtaataa ctgcaccgat atttcctgct
   106681 gttgccagat aagcattgac gatcggcggc attggccaag ggaccattac aactgctttc
   106741 gccgcaaatc ctaaagcggt tgcaaaataa ccaatcgttc cagtgataag aggcgttaag
   106801 ataaacggaa tagctaaaat tggattcagc atgatcggca taccaaaaat aactggttca
   106861 ttgatgttga agatcccagg agcaagggat aacttggtga tttgtttcat atcttcccgt
   106921 tttccaacta acaatactgc aatcagtaaa ccgatcgtga ctccagaccc gcccatactc
   106981 atatacatat cccaaaatgg catagtaata atgttcggta cttcttttcc agcttcaaaa
   107041 gcttccgtat ttacagcaat tgccgctaaa agcaacggtt cccgtatcgg cttcaccatt
   107101 tgatttccat ggataccaat cacccaaaag acttgcgcaa taaacatcaa tagcaaaata
   107161 cccggtaacc cttgtacgac accttctaat gggcgttgta cgacattgta aatgatatca
   107221 taaaggtaca ttcctgtaag ttctttgatg ataaatcccg cagccgcgat acccgataca
   107281 gtcagaatcg tcgggaataa agcagaaaaa ctagcagaaa cattcgttgg cacggtatca
   107341 ggcattctca gctgtagttt cttctgtttc ctcaaccagc aataaagttc aatagaaagg
   107401 atggagacca acattcccaa aaataaccct ttcgtatctg tatattgttt agccaatacg
   107461 ttttctacta taacagtctg attttcattc actaattcat aaacagttgg attaacagat
   107521 aaataagaca tcactgcgat gataccaggg aatacaccat ccactttatt cagcttggct
   107581 aattcgatcc ctatcagaaa aacagcatag atggtcaaga aacttagtgt gacatagctg
   107641 atggcagatg cgataggctt taattcttct aaaaaacgca agaaacttat ctgtgctaag
   107701 ccattttccg agtcaaagac cattgctgaa aacaatgtgc caaaagcacc cgtaataatt
   107761 accggaatca atgcagcaaa tgcgtttttt atcaccatga tataccttaa attattgatt
   107821 ctcgttgcca cgttccctaa aacattggct aatttatcca taaaactcat tccagatccc
   107881 ctcattcctg cttcttctcc ttgacgtcat ctctttttgt ctattagtca gcgcttacat
   107941 ttcgatgaac agaaactatt tactcctctt ttttaccaaa tataaaacaa aaattcatct
   108001 gatgtattta tcataaaata catcagatga actgtcaatt agtttctttt tctcctatta
   108061 ttttctgtaa ttctctccga tttattgcca tatggacagt cattgcatca aacgcagcaa
   108121 gtgcattccg cctttcgatg gcctgaacga tctctgtatg aatatcgatc gtctcgattt
   108181 tcagatgctg gatatcataa tttttattaa acaaagcaat tgattcatta atgattggga
   108241 taatatgcag caaggctacg tttccgctag cttctgcaat catcgtatga aaatcgatgt
   108301 ctaattgaac atgttcttgt gtgccttctc gaaaagaagt ttcaatcttt tgcgttaatt
   108361 ttttcatttg gacgatttga ttcttcgttg catattccgc tgccaaacct gccatcttag
   108421 gctccaataa atatctcagt tcaaataagt ctcgtatcat ccgatcctga tcttcaatca
   108481 aagaaaaacc gagtgggtca ttggcaatcc ctcgcttatc actgacaaat gtccccgctc
   108541 cttgccgaac ttcaaggata ttcctggaag ctagagcccg cacagcttct cgcaccgtgc
   108601 ttcgccccac ctctaaataa tcggacaatt catattcatt cggcagttta tctcctactt
   108661 gcaactgcat ttcctttaaa tatctaagga tcttgtcagt agttacttct actagggttt
   108721 tattttcttc caaaccacat acctcctttt catctgatgt attttttcgt aattattata
   108781 acacgtttga atcatctgat gattttttgt atacaaaatt gattaatccg ttcatagaca
   108841 tacaaacgaa gaagatattt accataaaaa agactaggat aaaattcact cctagccttc
   108901 gatcattttt attttgaacg acaaaaaata tgatgttcaa taacagacga tcttcattcg
   108961 attcttgatg tcatcttttg ttttttttaa tatatggata aaaagtttga ttacatcgtt
   109021 tctattctag aatttggtcg attctcaatt cttcttctgg tgagaaatct aaattatcca
   109081 tcattcgaac actttcttgt aactgactta accgacttgc tccgaccagt acacttgtaa
   109141 cagatttttg tcgcaaattc catgcaaggg ccatttgcgc taatgattgt cctcgtgata
   109201 cggcgattgt ttgcagtgct ttgatttttt ccagagttga tccaacttgt tcttctgaaa
   109261 ggaatggtat ttccttacga tgtgctctag aatcttcagg aataccatgt agataacgat
   109321 ttgtcagcaa cccttgagca agcggactga acgtgattgc ccctagccct tcttcttcta
   109381 atactggaga tagatcgtct tctagtagtg cacgagaaaa catattgtag cgcatctgat
   109441 gaataatgaa cggtgcttcc tttcttttta aaatttcagt catttttttt gtatcttctc
   109501 cattatagtt ggatatacca atatataatg cttttccttg tcttacaagt tgcatcaaag
   109561 cttctgctgt ttcttccaaa ggtgtatcag ggtctgggcg atgatgatag aagatgtcaa
   109621 cataatctag ccccattctt tggagacttt ggtcacaact tgaaatgatg gatttacgag
   109681 agccccattc cccatatggc ccttcccaca tatgataacc tgctttgcta gaaataatca
   109741 gttcatcacg gtaggcttga aaatcttctc taaaaatacg cccaaagttt ttctctgcag
   109801 ctccagcagg gcctccataa ttattcgcaa ggtcgaaatg ggtaattccc atgtcgaatg
   109861 cgcctcttaa aatctctctt tgattatgga taggatcata atcaccaaaa ttttgccata
   109921 atccgagaga taaaatcggt aatttcaaac ctgaatgacc ggatttacga tattccattt
   109981 tttcataacg tgcactatct gcaatataca atttttctcc tcctcatcaa atctttttaa
   110041 aacgctttct tttttctatt tttagtttac tacattttaa agaaaaggag ttaatcaaaa
   110101 tgattttttc gtatattttt ttgccatttg ttttttactc gttgatagat cagaaattga
   110161 ttacgtgctt gctggatttt tttataacgt ttcggttgtt ttttgtaaaa ttgctgatga
   110221 tagttttctg ctggccaaaa cgtaccggct ggttcgattt ttgtgacgat tggttggtgg
   110281 aaagttcctg aactgtctag tttctgtttt gattgttccg ctaattcttt ttgtcgttcg
   110341 acttgataaa aaatcactgg acgatattgc ttccctctat cttgaaattg tccaaatgta
   110401 tctgtgggat cagtaatttg ccaatagatt gtcagcaact cgctataact tatttctctt
   110461 gtgtcataaa tgatctccac agcttccaca tgacctgtgt aaccaccact tacttgatca
   110521 taatttggtt tttcaacatg tccgcccgtg tatcctgaca aaacagatag gatccctttt
   110581 ttcgattcaa acggctcgac catacaccag aaacatcccc ctgcaaatac tgcacgttct
   110641 tgatagtcag catcttgcat ggcatgcttg gaaaagtcat atttttgctc cttatcaaat
   110701 ccttccgtaa tcaataaata aaagtccatt acatcaggcg tcagattcat tcggatagct
   110761 aatggacgta gtgttgcttc tagttttgat aactgttctt taacggatat attttctctt
   110821 atgtcattct ttgtctgaat caatactttt cgttcccaat ctcgtgtatt cggattcaaa
   110881 atcagattat aaagtgtttg aatgaatatt tgttcctcat ccatagattc tcctcctttc
   110941 cgaagaagac aagctagtga ctttagctgt aatttatcat aaagtgagga aataagcgat
   111001 tacttcatcc ccctacagat ttttcgtata aaaaagagag aatccttatc gacagattct
   111061 cctttttaat caatttattt tataaatgaa aagttaattt accttttatg gaaaactatt
   111121 ttaaaaaata aacataaata tgcagttaat gtaacaagat aagaaataat agtcgttact
   111181 gtataacttg tgcttacttt aatatcagca actcctttga ctccgcttat ttgtaccaat
   111241 ccattagcat tcacctgctc ctttacaggt tgatgattta tttctacatt cgttcctaaa
   111301 taagcatata caggaagtgt gattacttgg ctcttctcat tagttactct atatatgata
   111361 gaactaccag ttgttttata aggaaggttt acccattgac tgccaatctt tgcttcgtgt
   111421 tttattatat gctccttatt ctccaatgaa gcgataggag aataatcata taaaaaataa
   111481 tccattcctt tagtggtata cgcctctact gtttctttgt ttacaacaat atgtcccggc
   111541 tgagaaatta ttttttgatt gaaatttttt ttactagaaa cagttaaagt gattaataac
   111601 aaaacagtca taaataaata aaatagttct cttgtcttat tgtatcttct atttttgaaa
   111661 aatatcatcg ttaatatcag agaagagaat attatttgca agcttaagat ccgccaagga
   111721 aattgtaaca atttggctgg cgtattttgc agaagatacc atggcaccaa actagttgta
   111781 gctagccaag tcaaacacgt accataataa atcttttttt ctatgtttgt taatttttta
   111841 aataaaatca ggggaataca taaagataac agaccaacta gtcccattga gtaagtagtt
   111901 aagtcgttgt tcatagcttc agtcagacta tcgaacacat tagatgcatg agcatataaa
   111961 tttgtcacgc taggtttgtt gattttttgg tagagcattt gttcaaacat cggataccaa
   112021 aaagcactcg tcagaaccaa tgtcattcct gccgcactaa ataatttttt aaatatagca
   112081 ataaaatctg cattcttgaa acctttagaa accaaacgaa taaaaataaa aatgataatt
   112141 aatgctgtac tcattaatgt tgatagtaca tggctgtaca ctagcaaact catactcaat
   112201 gctagcttta cagacggata cttttcttct tttccagaga tgatttgata agcataaaat
   112261 aatacaggga taacaagtgt tattgcgata gcttcggcaa ttgcggaacg atagtagatg
   112321 tctactgtcc gataagcaga gaaaatatag agacaactaa ataaaaatgc cgcttttttt
   112381 tgttttaaaa accgctcgcc ataatgataa gctacgagtg ttgtacacaa gttcaataac
   112441 caaacgtaaa taatatagct tacaatcaaa ttttttgtca tccaatataa cattacagct
   112501 ggaaaaaaag tcagaaaggg ataaaaataa tttacaccat atccagtata attgaatgtc
   112561 tcataattaa tcggactggt aaaaatagtc gataacccct tgattcgaga taagtgaaaa
   112621 gaaagatctc cagtggtata aatactctgt gtatttttga aaaagatgaa agaatatata
   112681 ctactaacta taattaatcc actcaatatt actacttttt tatttatttt cattcttctt
   112741 gtttatctcc tttttagtca catatcgaga atctttcaac ttattaggtg caaatcgttt
   112801 aaaacttgaa tacaccacta aacaataagt cagaatactg atccataaag ccatgagagc
   112861 agttggagta tattttgttg taatttctat cttattttct ccagcattta catccacatt
   112921 cactaagcca tgttttttat aagagtcatt tacgacttta ccgtttactt ttacctgtgt
   112981 acctaaataa gcatatactg gtactgtcac tttttgcttt ttgttgcttg ccactgtgta
   113041 agtaatgtta ttatctgcag ctttatactt ccctttttcc cacgattctc ctacttttac
   113101 ttcatgtttt tctaagtgag gcctttcttt tatcgcttct gttggggcgt aatcgtataa
   113161 tcctcccata tctgatgttg tataacgtgg gagtgtttct tcattgataa caatatgacc
   113221 gtgttttgat tggataactt ttgcgtaatt ttcttttgct gctatagacg tgacgagtag
   113281 aaacaccaca gtagcgccaa tgattaacca tgaatatttt ttattcatag tctttttcat
   113341 aaaaacaagc gttatgatca ttgaagaaaa aaagatttgg atacctaaga tacgccaagg
   113401 aaactggagt aagcttatcg gtgttttttg aaacaaaaac catggaaata aattagttac
   113461 gcatacccat gtagaaacta cgccaacata gatgattttt tcttttttct ccatttcttt
   113521 cagtatgaat aaaggaaaaa tcaacgcaac caatcctaat aaccccatag tgtatgttgt
   113581 aatatcgtta tttaacgctt gaatgatact atctgaaata gaaagtgctt ctagctgcaa
   113641 atctgttttg aagggactgt gtatttcttg ataattcatc tgctgcaaca taggatacca
   113701 aaaatagcta gttaatgcac aagtcaaaaa aacagccttt actaaattca cgaccacaaa
   113761 ttttatatcg ttttttattt gcttgcttaa aaacaaacga actataatca tacaagcaat
   113821 caatgcacta cacaatagtg tagttaagac atgagtatac acaagtagac ttatactgac
   113881 agataaagcg atgacttttt tttctctatt gaatatcaat ccataagcat aatataaaac
   113941 agctgggata atcgtaagag cgattgcttc tgcaattgcg gatcgataat agatatcaac
   114001 tgttcgataa gttgcaaaag tatataaaca actaaataaa aaagcagcat ctatcttttt
   114061 aaaaaacttt tctccataat aaaaactaat cagaatcgta caaatattta ataaccaaat
   114121 atatagaaca taacttaata ttaagttatt tgaaattcca taaaaaacga ctgcaggaat
   114181 tattgtaaga aaaggataaa aatagttcag tccatctcca taattgttaa atgttgtata
   114241 gttgattggc ccttcaaaaa tagaagataa ccctttgatt cgtgaaagat gaaaagataa
   114301 atcacctgtt gtataaattt cttgagcatt tctcataaaa tttactgaat ataaagtact
   114361 aagtaagata agaactacta aaagaaatgc ttttttactt attttcatag atttgttgtt
   114421 ctcctttttt aattctattt tcagtatact agaattgtct ttttttaata agttttgcgt
   114481 taaatttttc atagcactag tatgatatac tagaaaagaa tcaattttta ttacttttaa
   114541 ataggagaaa aaaatgaaaa aaaacaaaaa ccaaataata gatatcattt ttatgttatt
   114601 tctaagtttt gcttacgtca tccctctatt catgtctaag ggaatctatc atagtgtcaa
   114661 tcaggatact tactttcatt tatctcgaat cataggacta gataatgtat ggagttctcc
   114721 agtaaacttc aataacttcg atcatcatgg aacaatgatg aatatttttt atccatggct
   114781 tactctttat ccagcttttt tattttataa aatgatggga aatcttgttt taagttataa
   114841 tatctactat ttttttatca cttttttaac aatggtcgtt tcttattttt ctatgaaaca
   114901 aataaaaaac aatcgttata tttcgctgtt attttcgatt atttatacat tttccgccta
   114961 tcgtgccatt gacatattcc atcgtgcttc attgggagaa gctgtcgctt taacattttt
   115021 gcctttgata ctaatggggt gttacgaaat ttatattaga gattaccaaa aatggtactg
   115081 gttgtctatt ggcatgacgc tagtagtgta tactcattta ctttctgtcg ctatggtttc
   115141 tgtatttatt ggaggaacgt tgtttctttc tttttatttt tgggaccaaa aaatcgctag
   115201 actcttaagt cttttaaaag ctaccgtcct cacctttttt ttaagtgcgg gattcttaat
   115261 tccttttatt caacaatcta gagcacaaga attgaaagtc ccactgggaa aagaattaag
   115321 tggtatggct cctagtgata tgctgacaca tattttaaat aataactata acaactatac
   115381 tattggatta ttcttatttc tcggattgat tggtgctttt atttttataa aaaaattaac
   115441 tgctgatgat ttatttattt tctttcttgg tgtatttgtt cttttttgtt caaccaattt
   115501 atttccttgg caacttttta accatacacc agttaagagt ctacaatttg tctggcggtt
   115561 aaatggtttt tcttctttat ttatagccta tacgatgagt atagtgatct attatatttt
   115621 ttctactaaa aataattcat ggaaaataat ggtctttacg tttcttgcac taatattgca
   115681 tctctcagga gtaagcaatt ctatccatgc taacaaagat tcattaacac ttatagcacc
   115741 tgaagacgct gttgctatag ctgaaaatta caatcatact gattatgcaa acaaagagtc
   115801 aatctatcat cctgatatga ttaataatga tcaatattta ctaggtaacc aggaaataaa
   115861 tccaacaact cattttatga gtaacaaact tacaattgaa ttagataatt cgaataataa
   115921 aaatactgtc ttgacgacac ctatttatcg ttataaaggg caagtggctt ctattaatgg
   115981 caaactagta caaacaaaat tatctaagtt tggcacaact gaattaacta ttcctccagg
   116041 tataaataaa gtcgttatta cttatcagta cacaaaacta gcaattgctt caagatattt
   116101 atctatcgtt actttgattt tatttttact ttatcgattt actttttcta aacaaaaaca
   116161 accgagaaga gaagtacaac attctcacta gatttttatt taagaaagac tttttagctg
   116221 tacacataaa caggctaaaa agtctttttt tatatttctt taatttgctt catacattat
   116281 ttaacatcga tatttatact gtgaaacatg cgtttcatcc attatcaaag tatttttatc
   116341 gataataaac ttcatccaaa aacagacctt gtgcagggac ggtttctcct gcatgttgcc
   116401 gcactttgct ttcaaatatt tcatcgattg tttctatcgg catggcacct gtgccgattt
   116461 ctagtaatgt ccccatgata atccgcacca ttttatataa gaatccgtct ccgacaaaag
   116521 taaaatgaag catttctccc tttcgttcga tagataattc atcaatcgta cgcactgtgg
   116581 atttttttga ttttttcaat gcagaaaaac caatgaaatc atgtgtaccc actaattttt
   116641 cgcaagctgc attcagcttg tccatatcca actcttgagg gaaataaaag ctgtggtttc
   116701 gctcaaatgc tgaaggaatc acgttgttcc aaacataata gctgtatttt ttgcccaccg
   116761 cattatatct tgaatggaag cgctcaggca tttcttcgat tcttttgatg acaatatccc
   116821 gcggaaggta acgattcata aagtcgatca tttctgccag tccaatttct gtgtttgttt
   116881 taaaatttgc tacttgtcct cgcgcatggg tgccagcatc tgttcttcct gatccaataa
   116941 tttctatctc ttcatttgtc atctgatgca atactttttc gatttttcct tgaatggttt
   117001 tatcgtcatc tccaagtctt tgccatcctg aatatctttt tccgtcatat tcgattgtca
   117061 acttgatgtt tctcatattt tagtggctcc tctgtcttcc atttggtctt ctctctacct
   117121 ttttaagatt gtagcacaaa atatagctcc ttgcttctct aagacagaag ctatacaaga
   117181 aaactgtgga aatatatatt tcagaaaaac aaaaaacagc caccggatga agtatccgct
   117241 gactgttcaa tttgacggct caagttttac tgtttgagat gcaagatact caacctggca
   117301 atgggcacat aattaaagaa aaaactgatt ctttggcaaa aacctatttt attcagtaat
   117361 ggccaagaat ttatagaagg aattcttgcc agtccataaa tcgccgcaaa aagcaaactg
   117421 atcacagaca gtccaaggta taagtggctt acgtccattt gcccattttt tcgatagatc
   117481 aagaaaagaa gaactggaaa caataaaaaa ccactgtaac ctaaacctga tccgatatta
   117541 tggacccatg tcgagacatt ccacgaagat tcatgggtat tgatactgaa caaacctgta
   117601 aagatacaat cacctatacc atagaacgcg atggctgctg taactagggt agctaacaca
   117661 cgagatgtac taacaaatgc agcatacaat gcaggcaatg acaggatgaa aaagatccct
   117721 gaaaccacag accaaataag aaacgctttt cttacaggac tggaaacctc tccaaataca
   117781 ctgatcacgc tcttcatttg atctaattca ggataataca atcctaatac gtacggagtc
   117841 aaaaaatcac tcagcacgcc taataataaa aagaaaaaac caaatttacg taaaaaatcc
   117901 atgatacgcc tccaataaca attacctatg cttttaattg ttattataaa cgactttatc
   117961 ttgatttaac agaaacctct atttacacga acatctatat aactcattaa aaactcgaat
   118021 cattttaaaa atactaaata tctaacgttc aacgttcact taatccatct aaacaatctt
   118081 tcaatttgat tttttcccca cttcttttac gcgcgcctag ctctcctatt gcagtaaaca
   118141 acagatcact tgacgaattt aaggcagttt ccattgaatc ctggataaca ccgataataa
   118201 atccaatacc gacgatttgc atcgcgacat cattagagat accaaacaaa ctacatgcta
   118261 atggaatcaa taacagtgaa ccgcccgcaa cacctgaagc tccacaagca gctaaagcag
   118321 acaaaataca taaaaggact ttcaaaacaa aaggtgcttc aatccctaaa gaatgtacag
   118381 cagtcaacgt catcaaggaa atggtaatcg cggcaccgcc catattgatc gtagcaccta
   118441 acgggatcga tatggaataa gactcttttg tcaatttcaa tctttcagcc aacatcatgt
   118501 ttatcgggat atttgcggca gaacttcggg taaagaaagc gggaagtgcg ctttctttca
   118561 aacaaaagaa aatcaatgga tagggatttt ctttaataaa caaaaacgtg aggaatgggt
   118621 acacaactaa tgcgacaaac agcatcgtac cgatcaaaac aagtaaaagc tgcagatact
   118681 ctgccaatcc agcgattcca gtcgtagcaa ttgattgata aaccaatcct aaaatcccta
   118741 tcggagcaaa tgcaataatc acttgtacga cttgtgacaa cgctatagat atctgatcca
   118801 ccaactcttt tgtcgattct gaacgtgctc gaaaagctag tcccaacaat acagcccaga
   118861 ataagacgcc aaggtaattc gcatcaataa gagattgaac aggattttgt gtgacattcg
   118921 tcaacatagt agataaaaca ctttccaaac tttgcggtgc tttttcagat actgtttctt
   118981 gtaaagtaat aggaacagta aaaattgaac ttacaataac tgctacaagt gcagaaagca
   119041 atgttcccac taaatataag atcaaaatag gcttcacgaa tgtcttattc ccaattttat
   119101 gcttggcaat tgacgccatc gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc
   119161 aactgacaaa caacgttccc aatattgaaa taaatgtcca tgtggggaaa aagataccaa
   119221 acaatattcc gactaaaata ccaacaacaa tacgttgaac taacgacata tccaaaaact
   119281 tctctaacaa atgaccaccc tttcacaatt ttaacaatca ttgtatcatt ttatgcaaag
   119341 aaactaaagg tataattttc taaaaattct aacaatacaa acaaccattt cttgctaatt
   119401 ttgtactttt tctgtctaca cgttgttttt tgagatattc taagttagga tgttattaat
   119461 gggcaactta tcaggaaacg ttttcttatg tttttgtcca atttttttgt gaggtgttta
   119521 ttcttgaaaa aaataaagaa ggagagttat ggcttcctga caatctttgt cgcttgtata
   119581 atgatggttt tacagcgtat attgatttca gcagtcacta ttcctacata tgcaacaaac
   119641 cctggtcgat tctacatcta tacattttta caaacttccg ttgttattgg tgcaaatttg
   119701 atcccacttt acatgggtta ccaatatcaa aaaatcgccc gtttaaaagt tttacattat
   119761 ttaagtcgtt ttgcctttat attcctaata gccacactag tttgcaatat ctttttttac
   119821 gtacaagctg gcagcttgaa tattcgtgat tattggctta tcctaacacc tatcagtcag
   119881 aattacttta cttatgctgt ttcttgtact ttgctttttt gtagtattcc ttatacagtt
   119941 ggatttttaa ataaattatc gaaaacaaca attaaatatc tactattatt tttaacaatt
   120001 ggattagttg gctgctctac tctatttaat aaagacccgt ttgcattaca gaatggacag
   120061 agtattttat ggatttttta tttattttta atgggttatg gtttgaaaga atggaactgg
   120121 aaagaaaagg tccgctataa gcttcctcaa gtatttatca gcttgattgt tttattcggt
   120181 ttgattattt tgatgacaca aatttcactc atcatcagag gaaatacaga tacagcgctg
   120241 aggttttcta aaccatattc tctcttcgca atgtattata ctttctcttc gtttttattt
   120301 ttagaaatgc tatctgataa attaggtata aaaggaagag gaaattttct tgctgtattt
   120361 ttgattgtca ctcaagttat atgtaatttc cctttagttt cttatcgcgt atctacattt
   120421 atgaaacgtg aatttccaaa ctcagggaag gcatggctga taaatatcgg gcagtttttt
   120481 atttgatatt tattagcagt gtccctccta gttatctttt tattttatat acaaaaaatc
   120541 cgtttcattc atcgtttcat cgaatacttc gcttttgatt cgattgaaca attaactcag
   120601 cgttggaaaa aaagaattca ttggcttttt gttcataaaa gttatttttt agttgcagca
   120661 tttttttact gctttacctt cgtgcagatt ttcgttttag aaccaaaaga aggttggaaa
   120721 gaaactatcc aggttgcctt aaaaatattt actcagcgac aagcacccat gatcctgaca
   120781 atcatcatta ttatgggatt tttcttctta ctattgttga tttccaatcg tttttggtac
   120841 gccttgactg ctaccttgat cattaactta ctgctcacta tttcgacagt aatcaaaatg
   120901 gaaatgcggg aagagcctgt ctttccttca gatttaaaaa tgctgaccgg attgtcagaa
   120961 ctattatcga tggttagccc agtcttatta atcgtgggcg gattgatcct tctcctatta
   121021 ttgattacta gtattattgt acaacgtaga ctacaaaaac agtattcctt gaagattcat
   121081 tggaaacgtc gatttatcag tatcgctgta ttattaggat gtttttcggg tgtctttttc
   121141 attaatcaca aaaattcgcc ttctttcttg ctgtttaatt tatttaaagt aaacaagact
   121201 ttctttaacc agcaaggcgc cgtaaaagat aacggaccag tcattcaatt cctaaacaat
   121261 atcgacatta aaatcatgga agaacctgct ggctattcga aagaaaaaat taatggaata
   121321 atgaaaaagt acaacacaaa ggcagatgaa atcaaccaaa caagaagtga ctggttaaac
   121381 aatcagacga tcgttctcaa tttaagtgaa agcttcagtg atcctgcacg tgtccctaat
   121441 ctgacagtac ctacagaccc tattccttac atcaaaagca ttataacaga aggaacagga
   121501 ggattgatgt tatccgtcgg ttatggtggc ggaacagcta acatggaatg ggaaggactg
   121561 acaggcttga gtatcagtaa cctgtctgct tcgttagtta caccttatac ccagctagtg
   121621 gagcgtcaga aaataagtcc aaatattacc gatctattcg atgaaaaaat agccatccat
   121681 ccttttacag ctgctcttta tcgcagaaaa caagtgtttg ataaatttgg ttttgacagg
   121741 ttctattatt tagacagtcc ggataaatta acttatacgg ataaaataga acaaagccca
   121801 cgcatatcgg atgattcagc atacaaagaa accttgaaag ctttacaaag caatacggaa
   121861 acgtcacaat tcatccaatt atccactatg caaaaccata tgccttatga tgattactat
   121921 agtgaattga actacactgc tgaaggaact gctgtcattc ctaatagaag aaaagaatta
   121981 caaacttata tgcaaggcct tcactatacc gatacagcag taaaaaaatt catccaagaa
   122041 atcgacaaaa tcgataaacc gattaccttt gttttttatg gtgaccattt acccgcaatc
   122101 tatagtggga atgattcgaa aaaatatgga ttagaacagc accagacaga ttatttcatt
   122161 tataacaaca agtatagtcg tgaacaagca gctacgttaa ataaagaagt tgttgctcct
   122221 aataactttg gtgctatggc tcttgagcat gcgaatatca aagtaactcc ttactatgcg
   122281 ttgttgacag aagtatctaa tcatctacct gcattaacaa ttgatcctac agaaagcttg
   122341 tctaatcgtt ttaatggaaa acaggtcttt gttacagagg atagtcaaat tatccaagaa
   122401 aaaaatttaa ctgagaaaca aaaagaaatt ctagaagatt atcgtctgat acaatatgat
   122461 cttgttgcag gtaaccagta cagtgcaaca tgggcgacac aaaaaataaa agactaacaa
   122521 ataaactgag caaggggttg tgataaaaga catgtcacaa ccccttgttt atctcattaa
   122581 tttgcttgtc cataaacgat ttgattaaag ccatttttca attcattatt cgctgtctcg
   122641 ttgaagtaaa gataattatc gggattcccc tgattttgtt gtgtcaccaa tacttttggc
   122701 tgattttgct gaaacccgaa tagatagacg tgctttttca gatatggttc tgtctctgca
   122761 tcggaataga cggctgtgat ttgatagtct gcttgacctg tcccactttc tgaccattct
   122821 gcagaaacag gcactccacc aatagccatc ttccactccc cgttcaaaat agcttgaggg
   122881 actttaagac catataagtc cacactcatt tgattcgtat aacttttgta ttgttgtccc
   122941 attgtttggc cccatttggt cataaatgcc tctaattgtg cggatttgtc tgcattccac
   123001 agtgtttgtt caacttgttc gctgctttct gaagaggaag atgaagcctc tacagaagaa
   123061 cttgaagtag atgtttgagt tgtttcagtc gttttggaag actctgacga agtagaggta
   123121 tctgctgttt ctgtacttgc tgcagttgac ttgctctctg tgctagttgt tgtagtagtt
   123181 gcaactgttg ctttcgctgt ttcttttgtt gattcttttt tcgattcatt cccgttggta
   123241 caaccagcta acaaaaaagc tagaccaatt gttactaaaa tccttttttt caattaaaaa
   123301 actcctttcg attactccca cttctttatt ctatcaaata agcaatgtga ttttattgat
   123361 ttttcgtatt ttctcatcaa tctttcaaat cttctaaaaa ctgagaagca cccgctgatg
   123421 ttgtctgtaa tacatcagcg ggtgcttctt ttattttaat cttcgtgatt ttccaattta
   123481 cctcacgctt tatatttgta tttgaagaaa catagacttt tagtcatcat cgtcttcctc
   123541 atccaaatct tgtcggacag tttttccttg gagtctttgt acttggtaga tgatttcttt
   123601 attgaaactt aaaagttcaa caagcaattg ttgcaagcca tatttttctt ctttttcagc
   123661 taatttgatt gcccgtgtga taaagacatt ttggaaatca aaatcttttg caaagttttc
   123721 caacattgtt gaggctggta aatatttgtt ttgaccatgt tcgtaaattt ttccataacg
   123781 catgacttct tctaaagtag acggaacgat ttcattttca atcactaact catgagagat
   123841 tttattgaac catctcgctt cttgatcagc atattctgaa aaaagggtac ttaattcttg
   123901 cgaagatgta cccaaaacat acaattgtgc ttggatcaat ttcatttctt ctaatttaaa
   123961 atttgctaat acatgcccca ccatggcgcc agcagttggt ttatgatggt cgatatctgc
   124021 ttgttttgtt tcttgaacaa atttttcttc cactatattc attgatttac gcctctttta
   124081 cttttgctga ttggacttca taacctaatt tttccacaac tgcagctaat tgttcagggt
   124141 ggacttcatc tggatcaaag tttgctttga ttttgctcgc attaaacaaa actttcgtat
   124201 tgcttacccc aggctgttga gaaaccgcat gttcgatttt ttgcatacag cttggacaag
   124261 ctagtgtatc taattgcata ataatttttt tcatcgtagt tcttccttcc tttttcttga
   124321 tagtcttatt ctagattaca gcttccataa aaaaattgat tcttatcaag ttttacgatt
   124381 tttcttcttt gctttcgtgt ccaaaataca ataaacgcat cgcgttaaag ataacgatca
   124441 agatactagc ttcgtgaacg aacatcccac tagccatgta aataaacccg gcaaataagc
   124501 cgttgagtaa aaataagacg acaccaatgg caatcactac attttcacgg gtatttaaaa
   124561 cagttttctt agccaagcgg taggcatgaa ccaacgattc aaaattagat tgcatcaaga
   124621 caacgtcact cgtttcgata gcaacatccg ttcctgatcc catagcaatc ccgatatcag
   124681 ccgcagccaa agagggagaa tcattgattc catctccaat aaaagcaacg cgcaatcctt
   124741 gttcttggta ttttttcaca aattgaacct tttcgtctgg taataattca gcatggactt
   124801 catcaatgcc taacatatca gctacataat cagcagtcat ctgattatca cctgtcagca
   124861 tcactaactg ttttgctcct gcttgtttca acttagctaa ttgttcagcg acttctggtc
   124921 gaatcacatc tgacacacct atgatttgag tgattttttt gtcgattgct acaataatga
   124981 ccgagctgcc tgtttgttgt aattgatgta aatcttgtac ttgctttggt gtgagagaaa
   125041 gtttgtgagc ggcaatcaat ttggtgttgc ccgcacaaac tgtatgacca tcaatctcag
   125101 ctgtgattcc ttgtcccttg attgtctgat tatctttgac tgctaattgt tgaaaatcaa
   125161 tgtctttctc ctgagcataa tggatcacgg cgcgccccag aggatgatcg gataacgttt
   125221 ctaatcgagc cactaaagct aagctgtccc cttcatcctc tgtatagttc ttgatagtgg
   125281 atacagcagt agttcctttg gtcaatgtac cagttttatc aaagacaaaa gtatcgattt
   125341 tggaaaaagt atccattgct tcaccgcctt tgactaagat gccccgtttc gctccattac
   125401 cgattcctgc tacattcgaa acaggcgcac caatgactaa agcgccagga caaccaagaa
   125461 ccaaaacagt gattgctaaa cgaaaatcac gactaatcag accaacaacc aacgcaataa
   125521 gtaaaacggt tggagtataa tactgagaaa atcgatcaat gaatttttcc gcatgggatt
   125581 tggtatcttg tgcttcttct actaattcga taattttggc aaaggtcgta tcatctccga
   125641 ccttagttgc ttcaatcgtc acgtagccat ctgctaaaat cgttccagag aagacttcat
   125701 ctccactgat tttgacgact tgtcgtgatt caccagtcac tgcggcttca tccgcatacc
   125761 ctttcccgtc aatgactcgt ccgtccacag gaatagctcc accagttttt actaagacga
   125821 tatcgccttc ttccacgtca tcgatttcca cttcttcagt cgttccatct tcttggacaa
   125881 cactagcagt agatggggcc atctcggtca aagccttgat tgactcccgc gttttggtca
   125941 atgttttttg ttccagatag ctaccaaaca aaaacaagaa tgtaacgata gctgactcct
   126001 tgtattctcc gatcataaag gcaccaagca cagcaatagt aacgagtaaa tcaattgaga
   126061 taactttgac tttcactgcc tgatacgcat ggatcataat aggtatgacc gcaataatag
   126121 aagctatggc tagagcaatg ttatagaaca agacaaaacc agtaaatttt ccgataaaac
   126181 caaaaataat caacacagct gataaggcgg tgatatgatt tcgatgctct tggatccact
   126241 tttgtaattt catctttctc cctcttttcc tttcattctt acgagactaa gtatagatac
   126301 ttatccatca taaaaaattg accactatca agtttagtca aaaaggcggt tttcttttgg
   126361 tagaatacac tacaaaaaat agttagcaag aagactcact aaaagtgatc ttgccaacta
   126421 ttgtcgtttc tatcgaaatt ttcattgaat gattttcctg tacagcatac atcggagatc
   126481 tatttcttta tctaagattt ttatcttgct aattttttcc tttagtcttt caaatccacg
   126541 tcgttcatag aattgatacg tacagccact atctgtaaaa aggaatattt cttttccttt
   126601 ctctcttttt tcaaattctg ttagcaactt ggtaccgatg ccttttgttt tcaaatcagg
   126661 atttgcagct aaaaaaatga tctctccatc tggcgaatgc ttctttaaat atgctgcaag
   126721 catttgttca tttgctgtct cgtaagctcc tttactttct ttagcaaata tattttgtaa
   126781 aaaatcgaat atcttaacat atatttgctg tccaaaagaa cgatgtttct ttttttctcc
   126841 ttttatctcc gccagcaaaa cacctgccaa ctcgtcctct gcatacaaag caagaatttg
   126901 agttgctcga ttcaactcca tatacaaaaa atatcttccg taaagattca gcagaagttc
   126961 attttccata tatctctcaa agttcattcc ttcaacagca aaatagatag cttttttgta
   127021 atctttcttt tctaagggtt taaaagttag tttcatcatt tcgcctcctc aacttcaatg
   127081 attgtgttat acttacatta taatagacac agtgtctact atctataatc aatcatctat
   127141 aagtttgtcg attaagcgac agctttgctt tgatatgtcg gataaggaga taggtaaatg
   127201 aaaaagacag atagacgcgt caaaaaaaca gaaaaagctt tggctgaaac actatccaca
   127261 ttattagtaa ataaaaagat tcaagcaatc actatacgcg aactaacaga aacggcagat
   127321 gtacatcgtt cgacttttta tacacattat aaggatatct atgatctata tgatcaatta
   127381 gaaagcaact tttttcggga tctaaatgaa attctctctt atgatccagc ccatagctat
   127441 gaggaactat acactcgctt gattgactat gtttacgcaa atactttttt atatcgctta
   127501 tttatcctca accatggtga tatttcgttt cagaagaaag ttaaacaagt tattgaagaa
   127561 aactatctga acatatggct atatgaagac aagaaaacga ttatcacaga agaaatgaga
   127621 ttctttacta cgtaccacgt ccaaggttgt ctatccgttc tcaatcggtg ggtagaaagc
   127681 aattacaagt actccaaaaa agaattatta gaactcttac agaaactgaa tgatcacata
   127741 gagaaaatca tgccttgaat aaaaacatac taagcttagt taagaagaac cgtaaccttc
   127801 tcgtataaca actgaatctt atttagccaa attgtttact gaagattttt tgagtaaaat
   127861 ttctataatt cctacttatt gaaaagtagt ttgtagtgct gcttctccct ctctaagtga
   127921 tatatcttgt tgtaccaatc aaattcacct catcattaat aaattacttt taggctaatt
   127981 tattacaact tataggttga agctgtaacc aatttatagt catattatat ttttatcatc
   128041 cagtttattg aattcttctc gtttacctca attcattttc aaggggaatc agctcatcat
   128101 ctaacatttt acgccatgct cgcttcattt cttgtgggta tttaaatggt ttcgcttctg
   128161 gatgtttggc tttatatcct tcgtaatcct tactgttgat aattgctttc atattaggaa
   128221 ttggatcttc acctggcata tattgcagat tagaagcatg taattttttc tcagaaacaa
   128281 accactcatt ggaaaagaca tgaatcaaat catcacttac ttgtgtaaaa tagcgacgtt
   128341 taatgacaaa gatatcttca ttgctatcta gtgtaccttc atttctttgt gcttaaattc
   128401 aaagataatt ttctcaataa agatttttga ttccagtaag attcctttac tccttttaca
   128461 acatagcgtt gtcctttgtt ttgaaacatc ttcttcccct ctttgccatt aaaaatagcc
   128521 caattctcta taaagaagat taagcttcat tattataata taaagtttaa atttggttta
   128581 ttacttactt tttcttattc tctagtaagc taatattgaa aaaaatgtaa tcagatcatt
   128641 atttttatat gttgtaactt cctgataaga aaactaatta aaagaaagag gtaactacat
   128701 aaactaatta aaaaagctaa cttcatagtt ggaggataaa tggaagaaac ttcaatgatt
   128761 gtttctcctt gtgttccttc aatcgacact agtcctatta tcaatctctc ttttttgtcc
   128821 atttactttt gcgactgttc cccaataatc aaatacagga agcgttattt tttgtccttt
   128881 agattcatgt actgtataga ctattttcga atccatcact tgataaggca tagtatgcca
   128941 ttccttgcca attcttactt catgattctg caaatgttct ttgtattgta atgcttccgt
   129001 gggtgcatag tcataaagtc ctccgggaag gagtgtcgtg taacgaataa gattttcttg
   129061 attcattacc aagtgatctc tctgagtgac aactttatgt tcatagtttg cttttgttgc
   129121 agcagataca gccaacagta ataaaacaat tgccgttata ttaactactt ttctttgctt
   129181 agctgatgtg ctcttcatga aaacgaacga taaaatcaac gagccaaaca gtacttgaaa
   129241 gcctaagatt cgccaaggaa attgaatcat ttgaatgggt gtattttgta ataaagacca
   129301 tggaaaaaat ttggtggata agagccaaga gactaccgca ctaaggtaaa tcactttttc
   129361 tttgcgatta aatcctttaa aaaagaagag tggacagatc aaagaaataa taccaatgat
   129421 tcctaccgta aaggttgtca ggtcattatt catcgcacca atcaaactat ccgagacatt
   129481 taatgcttgg tgttgtaagg tcgttttatc tggctcatta atcgattgaa ccatgatttg
   129541 tcggaacatc ggataccaaa agtatgctgt taaaagaatc gtcattccta ctgcctttga
   129601 caactccaca atcaaccgga tgctttcttt ttttcttgtt tcacttacta caaagccgcc
   129661 taagactagt aagccaacgg ctattacact cataaaagtg gaaagaacat gtgtataaat
   129721 gagtaagctc atactgactg ctaatggaat tgcacatggc tttttctcat aaatcagtgc
   129781 gtaagcataa tacataacta tcggtataac cgtaagtgca atcgcttcag caatagcaga
   129841 acgatgataa atatcaatcg tacggtaccc ataaaatgta tataaacagc taaatataaa
   129901 agccgcatcg attcttttta aaaattttag tccataatga aacatcagca tgatagtaca
   129961 aacattcaat agccaaacat ataagatata actaacgatt aaattattac ttatccaata
   130021 aaaaatgact gccggaaaaa aggtcaagta gggataaaaa taattcactc cgctcccata
   130081 atgattaaac gtcgtaaaat tgattggacc agaaaaaatt gaagataacc ctttgactct
   130141 agcaaaatgg aaggcaatat cccccgatgt agaaatagta tgaacatttc taaaataatt
   130201 gattgaataa agtccagcaa taattgctag gagaatggac aatattattt ttttctttac
   130261 ttgcattact taattatata ctcctttcat ggattgcttt atctagtata acttaaattt
   130321 taaataaatc gttatttatt tttactctca cttaaaaaag cactaaaatt ataagagagg
   130381 gaggtaatgg tctgaacacc aagaattgtt ccaacatcga attgcttaac taatgagttc
   130441 aaagcttaat tatgtggctc ttatatttac cgagcgtgga ttgcgatctc tttagcgaaa
   130501 caaacatctt tcataaaaaa aacagccata tccgctttct tggatatgac tatttactac
   130561 tactttacta tctttcattc ttcttgtctc acttatgata aggcgctgcg tatcatgagt
   130621 gaatgatatt aggaaaactt cataaaataa atcttttata catttccact tctagatggt
   130681 cattcatgtt agaagaatca aactattttt ttactgtcgt aataatatta aaacactaga
   130741 acaaatcaat taaattccat aaaatataaa taatagacct agcatcatct ggtataaaat
   130801 ggatgctagg tccgttatat ttactcaaaa ttgattctac gaatagatct tcttatcatt
   130861 tctattttag tagtcatgcg ttgttgttct tctaaaggtg gtaatggtat aagtagttta
   130921 gataaatttg taccatttac atttggttgc ccagttccgt aagaaatttt ttctaataac
   130981 ttccaatata atggcgagtc taagaagcag tctacgtatt ctgataataa tttctcaact
   131041 aacctaattc gaatcaagta agaagcaaaa acagattcct cacttatttc cttaaccaag
   131101 aaactttttc ccatagttcc tcctgtcctt gctatcaaaa tgtcattttc ttccaatctt
   131161 aaatcaacta gtttagaatt actaatatca cagtaaggca cactggacca atttacacgt
   131221 ccctcttgta tatctgtgat tctcaaaaac ttaacattgc cttgtttttt cgccgaatct
   131281 gtatagccat attgaatctt agtagaaata cttcctaaat aaacccatag tcgaacatcg
   131341 ctagatgatt tttcttgtcg ttctttatga acaacagtga tcatcttgag ttgtccgact
   131401 attttttagt tgcagcaatc tttttgggac gcttatttgc atgaagcaac gttgctaagt
   131461 ctagtaataa aacattggct aacacaggat tggctttctc gcgatacgct gcggcgccac
   131521 tttcaaacaa tactgctaga cgatcttcta caacaacgat ttgttccaaa tacggtggaa
   131581 atttgattcg ttttaggatc cggctaggag agtcccgctc ggacactaag agttccgagg
   131641 tataaggacc ataagaacga ctggctaaga gataatggcc ataaaaagcc acaccttgaa
   131701 tcttatcaga tgtttttctg ctcattgtca cggttggttg gattttgtct ttgcggcgaa
   131761 ttgctgtttt catttcagct atcgtcggca atagatagaa agtcaactgt ccttcacctt
   131821 ttagtgtgaa gttgccagat accagttgat cattgttttg agtcaaatac gatgcttgtg
   131881 gaatctctga gagatcagtt gtcagccaat aagcgattgg ctgtcccgtc tcttcagatg
   131941 tatctgctaa gatatcgatc aaacgtaaag cggctactcg tccagtagca gatcctgtga
   132001 ttgtcaacca taaaagttcc cgttttgtat catatactaa gccccctaca tgtggccgtt
   132061 taggtaaaat cagcagtttg atcggctgtc ctgtttctct atccagtaca tgaataatag
   132121 aatgatgctg atggtcatag cagtaggcag aaataaacac aaaatcttga gaaaaagcca
   132181 acccttgcgg cgtcatccat tgacaaacct cattttcgcc ggtttccggc accaccattc
   132241 ttgtttctgt taatccagga atgttatatt gttttgcttc agccttttcc caagctgctt
   132301 gaacaatagg ataccgtttt aacttttttt caatatcctg atgtatccgt tgctgcttcc
   132361 aatgcttaaa ctttaggaca tagtaaacaa gaaggccaat gaccagcaac actaaaatca
   132421 ttattgctac ccgctgtgtc atttttttgc ctccttgttc tattcgatca atcgtccata
   132481 taccgctatt aattaccgta actacatgtt tcttttacta gcgtttcttt ataggatctt
   132541 tcataatctc atttatagta acattaagaa tcccttattc tactatagtc acttccacat
   132601 caatattgcc tcttgttgct ttagagtatg ggcaaaatgc gtgggcttct tctgcgattt
   132661 tctctgcttg gtccttctca agtcctggca aagaaacttc taaaacgacc ccgatttggt
   132721 agcccccatc ttctaattga aataaagata cccgtgcttt gacttcagaa tcagactcaa
   132781 gattattttc ttccagcatg tgttgaaccg cgccgttgaa gcaagatgcg tatccagccg
   132841 caaaaagttg ttccggattt gtcccttcac tatggattcc tgggcggtcg atcttcatgt
   132901 gcatacttcc gtttggtgcc tcaacttcgc catcacggcc tccacgattg atcattgctg
   132961 tttcgtacat ttttttcatt gagatcatcc tttctttcgt cgttctatat taagtgtaac
   133021 gagtattttt taatctgaca aaggtaatgc ttgcattcgg cgacatcagt tagacaacat
   133081 cgatgatcag tgatagaagc agtttatcta tacaaaaaaa gaagggttct atgatatttg
   133141 gtgtccttac tcgccttgag taacaacacc aaatatcata gaaccactta atcagcttca
   133201 cactgctttt tctactttcg tagttgaaaa aaacttaaac aaatcatcgg caaatagtta
   133261 aagaaaaaac ttaatcgttg aaagaagccc aattgcttaa agaaggcaaa ctgactaatc
   133321 gacggaatcc gtgctaatcc gtaaagacct gcaattagta aactaataac tgttaaaaca
   133381 agataaaaat ggctaagcgt accttgacca ctttgacgat aaagcagaac aagtaacaat
   133441 gggaaaagta gaaagcctgc atagcctagc ccagaaccta tattatggat ccaagtcgat
   133501 agattacaag agctttcatt cgtattgata ctgaataagc cagtaaaaat acaatcacct
   133561 atcccatata aaccaactgt tgcaacaaca agtattgcaa gtgtttttga tgttccaaca
   133621 aataggtgat acaacgcagg caacgaaaga acaaaaaaaa gtccagatac aactgaccag
   133681 accaagaaag cacgtctgac tggactatct acgtcaccaa acacactaat tacggtagtc
   133741 atttgattca gtttgggata aaaaaggcca aggatatacg gtgtcgataa atcactcatc
   133801 acacctaata ataaaaaata aaaaccatat ttttgcaaaa aaatcatttt cttcccctac
   133861 tttttagtga ttattcgata catccaacaa tcaaattttt tctcatttta acacatattt
   133921 ttattctagt tgtcttttga acaatcaata acataaaggt gagtttcttc tacattttaa
   133981 aaaatcgaat ggcactgaag agttcagttg tcagcatcaa tctcctactc aaaaaggcaa
   134041 aaaaacgtaa gtcagatgtt gttgacatct cacttacgca tccattgatt gaaaatttct
   134101 tagtaaaatc tactagccta aaaatttgaa gacttcttct cgatcataca gtttaaatac
   134161 tttctcgcat aaaaattgag taattagtgc cgcaaaaaca gggatgacta accaacaaag
   134221 aacaacaaga aagccattta gccctgcatc taaagaagcc aacggaccaa caagtccgac
   134281 cacgccgaaa ccagcagaag caggcgtacc tgaaatagaa aatagagcga caggaatagc
   134341 tgtaatcact gcactaacta aacaaggaat aagaatcaca ggttttctaa atagattagg
   134401 catcatcatc ttcatagcgc ctaaagcaat cgcaattgtc acacctgact tattaacctt
   134461 ccaagagttc actactaaaa caaaagtggt tgcagctacg cccatcgcag ccgcaccagc
   134521 agaaacacca tctagttgga ttgccattcc gattgctact gttgaaatag gagagataat
   134581 caaaaaggca aaggaacaac caatcaagat agacattaac agcggttgta aggtagtgaa
   134641 gttattaatc aatgatccaa ttgcagaagt aattgctgtt acaaaaggga ataaataaaa
   134701 accaagtaaa cctgcaccta cacctacaac aatcggtgtt aacacaatcg acactgaccc
   134761 aaaactattt ccaatcagta aaatcagccc gacagcaatc gatgctgtca gcatcgtatt
   134821 aataatatcg cctgtaccag caccaacgta aacacccgtt tcacccaaat caggtgtgaa
   134881 tttgactaca ccagaaccaa caaaagaagc tccggctaca accatcattc tcatcggatc
   134941 aaatccaaat tgtaaggcaa tcaaaccacc aatgatcaaa ggtgttgcta gttgaaaaat
   135001 aactgctaac tgtgcgatcg ttaccgcaaa gggatacgca ctaaaatatt ttaaaatggc
   135061 acctaatact gcattaggaa tcaatccaat aatgattcct gttgctgtac cagcgaggac
   135121 tttattcaga aaaattttcg gtgatatttt ttgtgaagtt tccattcaat ctctccttta
   135181 ttttacgttc aaactatctt cttttgcatg gacaagctca gtcaagaaat cacaataagg
   135241 cgtcgcaaca ccatatttct tcccttttct agaaattgcg ccattgatat aatcgatttc
   135301 tgttaatcga tggttcttaa tcaagtcttg atacattgaa ggataatgta acccaattgt
   135361 ttccgggtca aaacaagatt cacaatgtgc aatgacttct gggacatcta gttcaatctt
   135421 ctctactgct gctactttgg caaactcatt gacaatcgtc gccaccattt tatgagcagt
   135481 cgatgttttt cctagctcag ccatattaac gtctaagatt gtgcatagtc cgttcattgt
   135541 tccgttcaca catgctttgc gataaataga atagtgaata ttgtcagaaa aatgagcatt
   135601 taatcctgat tcagacaatt tgtctgctaa tttttttgca gctgcttctt ttccatcacc
   135661 taaattttgt aattctactg aaccacttcc aaataatttg acctgaccag gaccttcaag
   135721 acctgccgtc cacatggtat ttccaatata gatattttcc attggtacaa atttctcaat
   135781 aatatcttca tgcccgatac catttaatag acataagacc tctgtatctt ttttgattaa
   135841 tgattggata tcctgcagca ttttttccag ctgcatcgct ttggtaaata gaataatcag
   135901 atcaacttga tcttcttttt ctacttcgga ttgaagaacg attggtagtt ttgcttctac
   135961 ctctttacca ttaaaatttg cttgtaatcc atgttctttg atttgttgga catgttctgc
   136021 ccagccatcg attaataata cttcgttccc actttggtga agcatcaacc caaagcgact
   136081 tcccattgcg ccagcgcctg caattgctat tttcattgat tcttcctcct ttttctttct
   136141 attatactca tttgagaatc ttttcacaac ctcgttttgt tattcttctc ttatttatca
   136201 tgtttatatc caccattcaa gttattttat cagcaaatca ataaaaactg gaatagaatc
   136261 ccaatttcat cgtaagagaa actagactag caataagaaa atagaaacat tcatagaaaa
   136321 aatctcactc ctctgattat aaaagagggg gcattaacct cgtgtatact aaaaccttcc
   136381 attcattcga tggatgattt tctttttgtg aaaaaatatt cattgtagta cgatgttgcg
   136441 caacacagac ggatggtatt gcttattatt tattaaaatc gaacaataca cttatttaaa
   136501 aacttctttc ccagattttc ttccattttt acagttataa ctagtattat catgaaaaat
   136561 agccttttat tgtctatggg aataataatt taaaattggt acttttttat taattagttc
   136621 atcgcccaat aatctctcca gtttcttagc atctcctgac tcaaccggaa ttatccaagc
   136681 ccagtctaaa taatttttta aaatctcatc atattttgca aatatttcat tgccatattc
   136741 cgtccttaaa attatatttt cagtgccaga caagtgattt atattgagtt gataaaaaag
   136801 atctccatct aaaaaaatat cattagaaga agatttcccc acagtaatga ccattccatc
   136861 agctctatgt gttacataca aataattgta tgacctcatt tcacctaatg aatcaattat
   136921 ttcttgccct ttagctctaa tatattgatc tctattacct atctcactct tatttaacaa
   136981 aagttttttc atgtcaaatt taatcggctg ttttattttt gtaaattcgc ttctactact
   137041 aatccaatcc gagattaatt tttcaacatg tcccagagta aactctgatt tattcgctaa
   137101 atactttttt tgttgtatta aagacatctt tccccgaagc tcaaaattga gcctacatcg
   137161 attctctatc tcttcaaaaa gcatcttatc ttcatcattc ttatattcat cattcataaa
   137221 accaatccag ctcctcgatt aagataatat tttaatctca tacaaaaact agctctcatc
   137281 aaaacaacca ttgttttgat ttctcatgaa atttctttat acttttctcc gattataaat
   137341 tcctaacccc ttgagaaaat tacgcatata tttatctccg caaggcttaa aatttcgttg
   137401 gccttctttt ctcaaaaatg ccccgatttc gccttttgat ggatagattt ctgcctcagc
   137461 aaaaacgtct aaaatatcat cagtggtcat agacaaggcg atttttaatt tttttagcaa
   137521 cacattgttg atatatttga tttccttgct tttttgcggc gtgtctacta tcggcttatc
   137581 tttttgcgga ccacgcgcca agagaatcag gccatttagg aatgcgtcaa aaacttggtt
   137641 gttgatggtt ttttcataca cattattttc tgcattatct ttgttcttat cctgtacttt
   137701 ggtcagtacc gcctgagctt cttctttagt cagtggtaca ccgcctagct caaaagcttt
   137761 aatcaaatcc gtatctttca aatcaatcgc ataacgcaat cgcaataacc tatcattgtt
   137821 attcatttgt tcctccgaag tatttagtct ttactttctt acgtactttt tgtcttgcta
   137881 agatttttag gatcaatgat aaaaaccgca tcacttatga taaggcgctg ggtatcatga
   137941 gtgaatgata ttgctgaata caaatagtcc gaccacatta agttgatcgg acttcataag
   138001 cattttattg actaatactt ttttgcttaa gaattgattc taacatcttt tgctaaagtt
   138061 aagcatccta tagttcctgc attatctgct aacttaggtg tgacaatgta ttcttcgata
   138121 tttggcgtat cgacatagcc gttaattagt tctaaaaaac gtctttgtac tttctttttc
   138181 agatgttctt gttgcatgac accaccacca aaaattataa tatctggtga aagcatcagg
   138241 gtcgtattac aggcacattg tgcaatataa tccgcctcga tttcccagaa cggatcttct
   138301 atagaaagat cttgtccctt ggttcccgta cgcatctcga tagcgggtcc agatgccaac
   138361 ccttcgagac aatcatgatg aaaaggacaa gtaccagaga aagtatctcc tgaatgtctc
   138421 ttaactaacg tatgccccat ttctggatgg ctaaaccctt caacaaaaat cccatcttgt
   138481 atagctcctc caccaattcc agttccaaca gtgtaataaa caacactgga taaacctttt
   138541 ccatgacctg aaacatactc cccgtaacaa gcagcattca catcggttgt ccaggcaatt
   138601 ggaatttcga aatgcttatt caacgttcca acaaaatcaa aatttttcca ggcagttttt
   138661 ggagtagagg tgatatgtcc ataggtcttt gattcacgat ggatatcaat cggtccaaat
   138721 gaccctactc caatgctttc taattgttct ttatagtgat taaagaaatc aattaccaaa
   138781 ctcattgttt cattcggtgt agttgtagga aaactcactc tttctaaaac ctttaaatca
   138841 tcattgccaa tggcacaaac aaatttagtc ccacctgctt ctattgagcc atacatttct
   138901 tactccaccg tccttttatc taggaattcg ttcttccctg tctaatagtt tttcgaaaaa
   138961 ctcatgtggt tccttttggt accaataggc aacagaagaa atatcgtctt gacgctcata
   139021 aagcttgatg tcatcatttc ctagggtttg gaaagtaaca cgtaaatttt ctttgaaact
   139081 aatggggtcg ggtaaatgcc agcggtacaa cccatgcatc ggtaaagcat catcgccaaa
   139141 accatgaact ggatttggat taggttttcc agcactaaaa taatcccggg tgtgatctct
   139201 tttagattga aaaggatagc ccataaatag cgtattaaaa ttttgagcat atgccctttc
   139261 tccaaagttc tttttatgga aagcccaagc gccgccaaaa taatcttctg ctcctgtaga
   139321 tgttattgta gggaaatctt catctccatc tatataaaac ttaaattctc cttcccccca
   139381 ccagtatctt tccaatgcgc acaaagcaaa gtaggttcct acataatacc cagtgccttt
   139441 gattccatca ataagggtgt aatcttttcc ttttgtcgtg atgggttcac ggttccattt
   139501 agcatgaaaa tacatcgcat catccggtac ttcagtcaat gcataattaa atgtataaaa
   139561 aatgtgctta ataaactcag gatgttcatt cgttaaagta atcttagctt tttttcgaaa
   139621 tggcatctca aaatagcaat tcatccctga tgttggattc actacgattg gtaaggaatt
   139681 cacttcgcat cttgtcccaa acccattaca aaaaaaatct cctaatggac tttctacaga
   139741 aggagtggat tcatcatccc aataaatacg caaaattaaa tcacgtaaga cgaaactccc
   139801 agcgattgtt ttgtctgtca ctgtcatcca aaaatgacgt aaaattccag ggccttccat
   139861 ttctgctaaa gtaattgtct ctccagcatt tatcggaaca gaaggtgacc cttttctccc
   139921 aattcctaga tgactcgctg tcattgccgc tttccctttt tcaccatttt tattttctgg
   139981 tgaaatagtt ctactttcct ctcccttgtg gagccatata ttatttaaac aattcatttt
   140041 attcgtcctc tatcctttct tcactttttt gattattcat atagatgatt tgttgttgtg
   140101 cctccgcaat aattgctcca atatctaaat tatcatttcc taactgcaaa gcgattgaca
   140161 ttgccaaggg aagattcatt cctgcaatta cgtgcactct gtcagaaata taggtaaaaa
   140221 acttttgatt tacacttcct ccagccatgt cagttaaaac aattatttcg tctgtagtat
   140281 ccactaactc tttaatttgt tcatctatat catcaatttc ttcgacatat gcacataagc
   140341 tactaatttg attatgcata cctgttaaaa actctagtgt ctctttaaaa ccctcagcta
   140401 gtattgaatg gctagcaatg atcaatcttc tcataattca aatccttttc ctttacgcta
   140461 atattccaaa tgcagccata accatcgcaa aaacaataac gagtagaatc aaaatggtca
   140521 tactgatttt tttcttactg agtagataat atgctgcagc agtaataatt actggaatta
   140581 atgttggtaa aattttgtcc aacattggtt ggattttcat actaacttca ccaaaagtga
   140641 atttaagtgg cgttgaaaca ctgataactg aagcaattaa tgatccaact actgttagcc
   140701 ccaatactga agcagcttca gttaaataat taatcttttc accgtattca gtaacaatct
   140761 tcattccctg cttataacca aattcaaagc ctctcattcg gaaaaagaag attaccatga
   140821 ttaaaatgaa ccataaaaag attcctacag gattattttc taaagccata taagccgcaa
   140881 ttgatcccat gatggttggg atcatcgtca tcaaaagaga gtccccaata ccagcaattg
   140941 gacccatcgt accaacttta aagtcattaa tcgcatcttt tgcttctaat ccttgctttt
   141001 cctccattgc aacacaagca cctaaaataa tggctgccag ccaaggctgt gtattgtaat
   141061 actgaaagtg attatttaaa gcttcgatgt attcatcatc atctttgtat aattttctta
   141121 aaataggtga caatgcgtaa gtcacagatg caccttgctg tgtttgataa ttaaaggtac
   141181 aaactgacat caaccaacga tagttcgctt tcctaagatc ctttttcgtt acttgatatt
   141241 tttcgctcat tttattcatc ctccccaata aacccaacat cagaagctcc cgcagaagca
   141301 gtgatttgtg catctttttc agctctatga tacacattaa atgctaaagc gaatcctatg
   141361 atcgccgctc caataatagg taccttcaaa aaagcggcta agaaaaatcc aacaagtaaa
   141421 taactaatgt atcgacctgt aggcataaag cgtaacaaca tcgcaatacc aacagcaggc
   141481 aacatttttc cggctaatgt tagcccatcc ataaaccaat taggtaaaac atccaaaacg
   141541 taattaacag cagactgtcc taaagtaacg gcaataaata ctggaattgc tgcagataaa
   141601 ccaaagaaaa ttggccccaa ccacaaaaca ctattcattt tcttaaattc tcgtttattc
   141661 gcatattcct gtgatttgcg aacaacaaaa ccatttaaga ttttgacaat tacatctagt
   141721 tgcaccccta acatacctac aggtagtcct attgctaatc cggcttctac tccaagctgg
   141781 ctaccaaatg ccgttgcaat aattgcgcac gttccatagt ccggtacaga tgagccacca
   141841 aaaccggcaa ctcctaatga catcaattga actgtaccgc caactatcaa acctgttttt
   141901 atatcaccca tcaaaattcc tgtgaataat ccccaaaaaa ctgcgtaatt tacaaagatc
   141961 atcggaatcc cataataatc aacatgttta ataaatgcta atatagtcag cataataatt
   142021 tgaaaaatag taaaatgcat tatccttact cctttatcct aattttgatt gaatttcctg
   142081 tgccttatcc gccggaacaa attgtgtaac aatttctaca ccagaatcca acaatttttt
   142141 atacgtgctg atattttcat ctgatgcata ggctctagaa gatagctgta atgcattatc
   142201 tttaacaaca gtaccgccaa caattagtcg gtcaattcta ataccgttat ctactaaatg
   142261 aaggattgtt tccggctctt tcacgatgac aaatactttt tcctgatcgt atttcccctt
   142321 tttaaaattc gcaatcgctg tttcttcagt gataattgat acagccattc cagcaggtct
   142381 agctaaacgc atacttgatt tttggatgtc gttatttgcg acagaatcat cgatcaccat
   142441 cactctgttt gctttagaca ttggtgccca ttgcgtgaca ataatcccat gcaataaacg
   142501 gttatcaact ctcgacataa caatactcat aaatacttcc tcctttatct ttcattttct
   142561 tacaagcaat taccatgcca aaacaaaaag aacttgataa atcaagctct ttttacataa
   142621 tcataaacgt atttaacctc atgaatcggg atgtcgacac tataataatg ttcaacgaca
   142681 ctgaaagatt gcttaaaatg tgtctgaaat ttttgaaatt cctcactcgt ttcattgaaa
   142741 taaatttcat cttccaaagt attttgtttt actaatcgtt caattaagca actaatgtga
   142801 acatataatc cgaaacacgt attattatca aatgaaattc ctagatcatt ttgtaagatg
   142861 tatatagcat ctgaaacccg ttctaataat ttagtcggat ttaatatggt caattgctcc
   142921 attaaattcg ttagactaaa attcttcaga atattattgt tcaaaacttt cagttcatta
   142981 tcattaaagt gtctattcaa ttgattggag aatttactta tattcattcc taaaatcata
   143041 tcttcaattg aaaagaattc tacatcttct agatgtggat ttagtgttcc aacaataaaa
   143101 agaatattgt tctctttacg aagctccgat aaatagtttt cattcactaa agagtaatag
   143161 tcacacgttt ctatgacaag atcagtatcc cttggaaagc tatcttcaag tatctttttt
   143221 aatttaaacg aagttcctaa tccgcttgca caagaacaga caatcatatc cttcttaata
   143281 tttgatgatt cataaatata atccacattt tgattttctt ttacttgttc aagaatctgt
   143341 ttaagcggta aatctaataa tatctgattt gcaacatcaa tagccattct tgttgaaaca
   143401 ttgtttagta gtccgaattt tttttgagcg tctagttcca aatttagata aattttctcc
   143461 agtgaaccca tatcaacaag taaaataatt tcttcatatc cggtacactc ttgtaagtat
   143521 ctattaattt tgtctgcaat ctcactactt gaagtcatta gtggcatatc aatggcgtcg
   143581 tatatatatc tctcaacgat cctattagca gtacttgcta tacaggttgc cgttagatct
   143641 ccatgagcca aaatcagtgc taaacgtttt tgctgaaatt ctttactaca cattaactta
   143701 aataaaataa ccaaaacatt tttttgaatt tttgttaaat aaatatcccc tatttgaata
   143761 aaacgattga ctaattgaac ttctcgacta caccaactat taatatcatt gatgaaggaa
   143821 tataatttat cgtccgatgt taattggatt tgataaatcc aagaattcaa aaagatagac
   143881 gttatttgaa gataataata gtcaaattct atttgaaaat ctctctctat ttccttaaca
   143941 tactgtctaa atcgaatctc ttgataggtg ggatatgtta aagctgaatt attttggata
   144001 tcaacattct tttgtctaaa atatctatca tatttctcaa aaagctgctg cttatcagtg
   144061 tctttcttcc acgtttgata aagatactct ataagttcgt tgatattaaa aacttcgtct
   144121 actttttcca aatcatccat ttgaatatat tttaattcat tctgtctttt cataaccttc
   144181 atgccgttga aattcaccca ttgtggtaaa tgatacatct gaatataaag ttcttccaaa
   144241 tcatctaaga aataaaaggc attggcacag gtcaattgaa tgatattttt caaattgcca
   144301 acattttcac taaagcgata gtttaataaa aaatctaata attgttgaga tatcagtatt
   144361 ttctttgcta gtctttcttc ttctgattta aaaaatatct tgattaactg ttttctttca
   144421 taaagagaac gactgtctaa tgttggaagt gttagcttta caggtattct tctcaacaaa
   144481 gttgataata gtttatcctc aggtggttct gatgttgcaa atataatcca gcaacttgat
   144541 gtgtaccagt tttcattatc acccatccga tgaaatttcc tcgtatccat aaactgaaaa
   144601 agtttctcct gacattcatt gctcaatgca tgaatttcat ctaaaaataa gactccatta
   144661 ttagctaatt ctgctaaacc ctttcttcca tcttcggctc ctgtatatgc accttttaca
   144721 tgcccaaaaa aattggacaa gaaaagttct ggattattgg catattcact acaattaact
   144781 gaaatgaaag atgccttctc ttctatataa ttattggcca acaaatcctt atacacataa
   144841 tttgctaaat agctttttcc agttccagtc tcgccatgaa gtaaaatggg taagccattt
   144901 ggcggatagt agattgctgc tttaatttga gcaattacat ttcgcagact tccttgactt
   144961 ccgataaccc tttctattcc aaccctaaaa gagctatcat accatttttc cgcttcctct
   145021 gaagagctaa aaatttgttg atttggaact ttctccggta aagaaaagaa gattactgga
   145081 cgagtattga tttttccaaa ttctccatct tttacgaatt cattcaaata ttgagagaca
   145141 atattacgac taacatttaa tttttcagat attttctgtg ctgtattgtt cccccattta
   145201 tttttaatct cagagttctc tatgacataa ttgacaatgc gttcttttaa ttcttccatc
   145261 acagcacctc ctcgacacta ttcgaatttt gccgacagta tttttccata aatatgactt
   145321 gctaaaaagg ctttgtttag atcattcaat tggaacgttg cttgttttgc ttgatccgct
   145381 aagacgttga aaagatactc tggcgaaatc gcatacccca aaatatcaac gattgttgca
   145441 atcagatctt cttttgattc ttcatctgaa agtaattcac gatacaacat cgtttgtttg
   145501 cttactgtgt catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa
   145561 cggtcggata aatatttata gaaaatcagc cccaataaat aatttttata ttctgacgca
   145621 tccattttac tacggagatt atctgccgca ctaaataact tctgatttaa ttctgcaccc
   145681 aaaaagatac actcctatct cttatttaaa gttgctatat accctcttat tttagcatac
   145741 tcagttttag aaaaagcaaa aaaataaaga aacttattct tgataacaag ctacgtgacg
   145801 tatatcccta acacaaaaat agccatatcc tttggtttgg atacggctat ttgatattac
   145861 tcattcaatt atcaaatcac agtaggtgtg aatctcatcg ggatcggcaa tatctacttc
   145921 tgattaggat caaaggtgat tttctcaata aaggtgtgat acaaagcttt aacttgcttc
   145981 ttctcactct ggataagaaa gcaatccaaa ctagtcaata tctgttggac atctttgact
   146041 tgaatcggtt ccccttgttg ttgaaagatc cctaagattt cattctctcg aatttgtaac
   146101 tgtcttcgtt tttcagtcaa ttcatttaaa cgatctttta acatcggaaa caattcggga
   146161 ctatcttcca aagcagcttc ctgtttttgc tctaatagtt agattttgcg taccattttc
   146221 ttcttttatt ttccttcgat tgctgatttt aatatagtct aaaggaaatt tatatttatt
   146281 gtattcttta taacttcaag agaacttcgt ttgagtatta ctgtattttt ttaattccat
   146341 aagaaaatta gttattacct gccacctaat cagcaaatct gaacctgagt caatataatt
   146401 aatattattt tccatctcca acattgtaag tactgttggt gttaatatag ggactttgta
   146461 caattgtatt tttttgattt tatcatctgc acaaaggaga ttttttaaat ctaatccttc
   146521 ttttatttca aaagttgaat tttttaaatc tataagagta aaaccaattc cttcatactg
   146581 aatttctgaa aatcgaattt ttctcccagt tgatagatca aggggcagaa aaccttttat
   146641 tacattgtga gtgtcaccat catttacaat gtcattagga attttgattt tagaattctg
   146701 tggagcacct attggaagat aataattcat tataattttg tagtcttcat gaggaaataa
   146761 agtactttta ggattgctaa gatattctcg taccgaatct aagaatggct tatccattga
   146821 atcttcatcc ccatttaaac taaaaaacat tcctacaatt gcttttgcaa ctctaagtga
   146881 attcattttc tttattgaat gtgttaaatc agacttacca ttctttaaac ttgtattgtt
   146941 ataaaggaaa tttttattgt cttctgcaaa tctaatatat tcctcaacat actcactacc
   147001 aaaaaaacta ttacatttac tacataacg
//
LOCUS       DASJPC010000002        79991 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJPC010000002 DASJPC010000000
VERSION     DASJPC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.002"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            264..782
                     /locus_tag="U4A13_000133"
     CDS             264..782
                     /locus_tag="U4A13_000133"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1778467.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            910..1593
                     /locus_tag="U4A13_000134"
     CDS             910..1593
                     /locus_tag="U4A13_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN1778468.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1902..1975
                     /locus_tag="U4A13_000135"
     tRNA            1902..1975
                     /locus_tag="U4A13_000135"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1936..1938,aa:Arg,seq:tct)
     gene            2134..2493
                     /locus_tag="U4A13_000136"
     CDS             2134..2493
                     /locus_tag="U4A13_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN1778469.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2616..4247
                     /locus_tag="U4A13_000137"
     CDS             2616..4247
                     /locus_tag="U4A13_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN1778470.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4309..4950)
                     /locus_tag="U4A13_000138"
     CDS             complement(4309..4950)
                     /locus_tag="U4A13_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1778471.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5136..5212
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5232..5540
                     /gene="rplU"
                     /locus_tag="U4A13_000139"
     CDS             5232..5540
                     /gene="rplU"
                     /locus_tag="U4A13_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN1778472.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5567..5911
                     /locus_tag="U4A13_000140"
     CDS             5567..5911
                     /locus_tag="U4A13_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN1778473.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5924..6217
                     /gene="rpmA"
                     /locus_tag="U4A13_000141"
     CDS             5924..6217
                     /gene="rpmA"
                     /locus_tag="U4A13_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN1778474.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6651..9458
                     /locus_tag="U4A13_000142"
     CDS             6651..9458
                     /locus_tag="U4A13_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1778475.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9649..10140
                     /locus_tag="U4A13_000143"
     CDS             9649..10140
                     /locus_tag="U4A13_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1778476.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10243..11232
                     /gene="manX"
                     /locus_tag="U4A13_000144"
     CDS             10243..11232
                     /gene="manX"
                     /locus_tag="U4A13_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN1778477.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11265..12068
                     /locus_tag="U4A13_000145"
     CDS             11265..12068
                     /locus_tag="U4A13_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN1778478.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12089..13003
                     /locus_tag="U4A13_000146"
     CDS             12089..13003
                     /locus_tag="U4A13_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN1778479.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13086..13460
                     /locus_tag="U4A13_000147"
     CDS             13086..13460
                     /locus_tag="U4A13_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN1778480.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13599..13844
                     /locus_tag="U4A13_000148"
     CDS             13599..13844
                     /locus_tag="U4A13_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1778481.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            14016..15080
                     /locus_tag="U4A13_000149"
     CDS             14016..15080
                     /locus_tag="U4A13_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1778482.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15326..15751
                     /locus_tag="U4A13_000150"
     CDS             15326..15751
                     /locus_tag="U4A13_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1778483.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15744..16193
                     /gene="nusB"
                     /locus_tag="U4A13_000151"
     CDS             15744..16193
                     /gene="nusB"
                     /locus_tag="U4A13_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN1778484.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16290..17144
                     /locus_tag="U4A13_000152"
     CDS             16290..17144
                     /locus_tag="U4A13_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN1778485.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17145..18482
                     /locus_tag="U4A13_000153"
     CDS             17145..18482
                     /locus_tag="U4A13_000153"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN1778486.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18487..18711
                     /locus_tag="U4A13_000154"
     CDS             18487..18711
                     /locus_tag="U4A13_000154"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN1778487.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18713..19603
                     /locus_tag="U4A13_000155"
     CDS             18713..19603
                     /locus_tag="U4A13_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1778488.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19612..20430
                     /locus_tag="U4A13_000156"
     CDS             19612..20430
                     /locus_tag="U4A13_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN1778489.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20537..20986
                     /locus_tag="U4A13_000157"
     CDS             20537..20986
                     /locus_tag="U4A13_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1778490.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            21004..22686
                     /gene="recN"
                     /locus_tag="U4A13_000158"
     CDS             21004..22686
                     /gene="recN"
                     /locus_tag="U4A13_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN1778491.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22741..22857)
                     /locus_tag="U4A13_000159"
     CDS             complement(22741..22857)
                     /locus_tag="U4A13_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN1778492.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22930..23886)
                     /locus_tag="U4A13_000160"
     CDS             complement(22930..23886)
                     /locus_tag="U4A13_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1778493.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            24057..24425
                     /locus_tag="U4A13_000161"
     CDS             24057..24425
                     /locus_tag="U4A13_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN1778494.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24595..25026
                     /gene="mraZ"
                     /locus_tag="U4A13_000162"
     CDS             24595..25026
                     /gene="mraZ"
                     /locus_tag="U4A13_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN1778495.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            25047..26006
                     /gene="rsmH"
                     /locus_tag="U4A13_000163"
     CDS             25047..26006
                     /gene="rsmH"
                     /locus_tag="U4A13_000163"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN1778496.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            26011..26412
                     /gene="ftsL"
                     /locus_tag="U4A13_000164"
     CDS             26011..26412
                     /gene="ftsL"
                     /locus_tag="U4A13_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN1778497.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26413..28605
                     /locus_tag="U4A13_000165"
     CDS             26413..28605
                     /locus_tag="U4A13_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1778498.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28631..29593
                     /locus_tag="U4A13_000166"
     CDS             28631..29593
                     /locus_tag="U4A13_000166"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN1778499.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29607..30968
                     /locus_tag="U4A13_000167"
     CDS             29607..30968
                     /locus_tag="U4A13_000167"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN1778500.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30994..32082
                     /gene="murG"
                     /locus_tag="U4A13_000168"
     CDS             30994..32082
                     /gene="murG"
                     /locus_tag="U4A13_000168"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN1778501.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32097..33317
                     /locus_tag="U4A13_000169"
     CDS             32097..33317
                     /locus_tag="U4A13_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN1778502.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33473..34798
                     /gene="ftsA"
                     /locus_tag="U4A13_000170"
     CDS             33473..34798
                     /gene="ftsA"
                     /locus_tag="U4A13_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN1778503.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34821..36062
                     /gene="ftsZ"
                     /locus_tag="U4A13_000171"
     CDS             34821..36062
                     /gene="ftsZ"
                     /locus_tag="U4A13_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN1778504.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36079..36756
                     /locus_tag="U4A13_000172"
     CDS             36079..36756
                     /locus_tag="U4A13_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN1778505.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36770..37369
                     /locus_tag="U4A13_000173"
     CDS             36770..37369
                     /locus_tag="U4A13_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN1778506.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37384..37671
                     /locus_tag="U4A13_000174"
     CDS             37384..37671
                     /locus_tag="U4A13_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN1778507.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37720..38502
                     /locus_tag="U4A13_000175"
     CDS             37720..38502
                     /locus_tag="U4A13_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN1778508.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38523..39230
                     /locus_tag="U4A13_000176"
     CDS             38523..39230
                     /locus_tag="U4A13_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN1778509.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39492..42290
                     /gene="ileS"
                     /locus_tag="U4A13_000177"
     CDS             39492..42290
                     /gene="ileS"
                     /locus_tag="U4A13_000177"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN1778510.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42339..43865)
                     /locus_tag="U4A13_000178"
     CDS             complement(42339..43865)
                     /locus_tag="U4A13_000178"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN1778511.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43880..44527)
                     /locus_tag="U4A13_000179"
     CDS             complement(43880..44527)
                     /locus_tag="U4A13_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN1778512.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44711..45040)
                     /locus_tag="U4A13_000180"
     CDS             complement(44711..45040)
                     /locus_tag="U4A13_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN1778513.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45217..45945
                     /gene="sfsA"
                     /locus_tag="U4A13_000181"
     CDS             45217..45945
                     /gene="sfsA"
                     /locus_tag="U4A13_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN1778514.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45961..46974
                     /locus_tag="U4A13_000182"
     CDS             45961..46974
                     /locus_tag="U4A13_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1778515.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46974..48251
                     /locus_tag="U4A13_000183"
     CDS             46974..48251
                     /locus_tag="U4A13_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1778516.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48314..51016
                     /locus_tag="U4A13_000184"
     CDS             48314..51016
                     /locus_tag="U4A13_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1778517.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51168..51485
                     /locus_tag="U4A13_000185"
     CDS             51168..51485
                     /locus_tag="U4A13_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1778518.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51515..51835
                     /locus_tag="U4A13_000186"
     CDS             51515..51835
                     /locus_tag="U4A13_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1778519.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51943..53403
                     /locus_tag="U4A13_000187"
     CDS             51943..53403
                     /locus_tag="U4A13_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1778520.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53471..53692
                     /locus_tag="U4A13_000188"
     CDS             53471..53692
                     /locus_tag="U4A13_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778521.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53723..53905
                     /locus_tag="U4A13_000189"
     CDS             53723..53905
                     /locus_tag="U4A13_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN1778522.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53905..54318
                     /locus_tag="U4A13_000190"
     CDS             53905..54318
                     /locus_tag="U4A13_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN1778523.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54441..55622
                     /locus_tag="U4A13_000191"
     CDS             54441..55622
                     /locus_tag="U4A13_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1778524.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55693..55857)
                     /locus_tag="U4A13_000192"
     CDS             complement(55693..55857)
                     /locus_tag="U4A13_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778525.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            56011..56084
                     /locus_tag="U4A13_000193"
     tRNA            56011..56084
                     /locus_tag="U4A13_000193"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56045..56047,aa:Arg,seq:cct)
     gene            complement(56121..56321)
                     /locus_tag="U4A13_000194"
     CDS             complement(56121..56321)
                     /locus_tag="U4A13_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1778526.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56626..57858)
                     /locus_tag="U4A13_000195"
     CDS             complement(56626..57858)
                     /locus_tag="U4A13_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN1778527.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            58112..58681
                     /locus_tag="U4A13_000196"
     CDS             58112..58681
                     /locus_tag="U4A13_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1778528.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58859..59299)
                     /locus_tag="U4A13_000197"
     CDS             complement(58859..59299)
                     /locus_tag="U4A13_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN1778529.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59457..60221
                     /gene="map"
                     /locus_tag="U4A13_000198"
     CDS             59457..60221
                     /gene="map"
                     /locus_tag="U4A13_000198"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN1778530.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60253..61176
                     /locus_tag="U4A13_000199"
     CDS             60253..61176
                     /locus_tag="U4A13_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN1778531.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            61252..62391
                     /locus_tag="U4A13_000200"
     CDS             61252..62391
                     /locus_tag="U4A13_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN1778532.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62384..63184
                     /locus_tag="U4A13_000201"
     CDS             62384..63184
                     /locus_tag="U4A13_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1778533.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            63184..64011
                     /locus_tag="U4A13_000202"
     CDS             63184..64011
                     /locus_tag="U4A13_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1778534.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63989..64723
                     /locus_tag="U4A13_000203"
     CDS             63989..64723
                     /locus_tag="U4A13_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1778535.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64823..65689
                     /gene="rfbA"
                     /locus_tag="U4A13_000204"
     CDS             64823..65689
                     /gene="rfbA"
                     /locus_tag="U4A13_000204"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN1778536.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65703..66275
                     /gene="rfbC"
                     /locus_tag="U4A13_000205"
     CDS             65703..66275
                     /gene="rfbC"
                     /locus_tag="U4A13_000205"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN1778537.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66297..67325
                     /gene="rfbB"
                     /locus_tag="U4A13_000206"
     CDS             66297..67325
                     /gene="rfbB"
                     /locus_tag="U4A13_000206"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN1778538.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67423..68274
                     /gene="rfbD"
                     /locus_tag="U4A13_000207"
     CDS             67423..68274
                     /gene="rfbD"
                     /locus_tag="U4A13_000207"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN1778539.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68309..70342
                     /locus_tag="U4A13_000208"
     CDS             68309..70342
                     /locus_tag="U4A13_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778540.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLDFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70386..71666
                     /locus_tag="U4A13_000209"
     CDS             70386..71666
                     /locus_tag="U4A13_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN1778541.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            71876..72682
                     /locus_tag="U4A13_000210"
     CDS             71876..72682
                     /locus_tag="U4A13_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778542.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            72694..73914
                     /locus_tag="U4A13_000211"
     CDS             72694..73914
                     /locus_tag="U4A13_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778543.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            73904..75490
                     /locus_tag="U4A13_000212"
     CDS             73904..75490
                     /locus_tag="U4A13_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1778544.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            75529..77667
                     /locus_tag="U4A13_000213"
     CDS             75529..77667
                     /locus_tag="U4A13_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1778545.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(78035..79018)
                     /locus_tag="U4A13_000214"
     CDS             complement(78035..79018)
                     /locus_tag="U4A13_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN1778546.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            79396..>79991
                     /locus_tag="U4A13_000215"
     CDS             79396..>79991
                     /locus_tag="U4A13_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1778547.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEET"
BASE COUNT        26064 a        13523 c        16992 g        23412 t
ORIGIN      
        1 tacgtaaata aaaagattct tttttattat atatgttatt aaaaaaagac ataaaggtat
       61 tcctttctaa aacttgtaat gttagggtaa acgtttttta aactaacgtc aattatttat
      121 aataaaaaat gagataaaga tgaggaaata gaattagaaa catataatta ttaaagggaa
      181 acatttttac agctataatt tgaaaaaaat ttatcgttat tatatgatta agaaggattg
      241 aataaagaaa gaagttgaaa acgatacctg attacaaaaa aataactttt ataagttcta
      301 taacgttgat tatattttta attcttattc ttctcgtcgt cttggttccc cgaatttttg
      361 gtttgactcc ttatattatt gaggacaaag aaatgagtcc atattatgca caaggaaatc
      421 tgatatatgt acgaccgaaa aatacaaatg agattttagt cggagatatt attacatatt
      481 atgaaaattc aggaaaaaaa atctcgacgc gtcgcgtaat agccgttgaa aacaaccacg
      541 aagattttta tacaaaagct gatacaaaga cttatattga gcctggagta gtaaaaaaga
      601 gaaatattat cggtagtccc atctttcaga ttccatatat tggactagtc ctttcaaaaa
      661 cggcatttgc atggattcag gggttattct ttacgattgc tttttgccta acaggaatca
      721 cagcatggaa tacctttgta gagttgaaaa agaagcggag acgaacacag aaatttttgt
      781 aagaatgtta aaaacataaa tgtattccag agatccaacg actcttctga acatgaaaca
      841 cgagaaaaca ctcatgcagc ttgtagattt agtcagttgt tcgtataatg tgtgaagagg
      901 aggagtagta tgcgcaatga aatgatgcat cgtcctgtag tcaaagaaga actattggat
      961 tttatgcgta cccagcaaaa aaaattaccg ggtgagctag gcaaactgga agaagaagct
     1021 catgtagcag aagtaccagt tattccccat gaaacagttg tatttttgaa atttttactt
     1081 ggacagcttc agcctgaacg aatcttggag atcggagcag ctatcggttt ttcatcaagt
     1141 gtcatggcca cgacaatcgg agaaaacgct catgttacga caattgatcg tttcgatgtc
     1201 atgatcgaga aagctaaaaa aaactatgaa cgtctaggac tgacagataa agtgactttg
     1261 cttgaaggtc aagcagcaga tatcttgccg gagttgtctg gtccgtatga ctttattttt
     1321 atggatagtg caaaatcaaa atatattgaa tttttacctg aatgcttacg tttgttaaga
     1381 aaaggtggcg ttttgatggt agatgacatc ttccaaggag gaacgatctt gctcccagat
     1441 gaagagattc cccgtggcaa acgagctatc catcgtaaat taaatgaatt tcttcgtgtt
     1501 gtgatggatc atcccgattt gacatcgaca atcttgccat taggtgatgg tgtgatctta
     1561 atgacaaagg aatcagaaac aatcgagttg tgatacatga ttatggcagc ctgtagataa
     1621 tcgattgaat cgagggctca attcgaactt gatgtaaaag tttctatatt tgtaagtcaa
     1681 ttaaaggata caggaaatct aaataaacag gagtagcatg aagtcaatgt agctcctgtt
     1741 ttcaattgat aaccttagaa cagctgaagg agaatgtctc gttttaaaag attgatttag
     1801 tagttgggcc aaaataattc attatttgct aattaaataa ctatttcgta ttgacagaag
     1861 caatttaatt atctataatt attgatgttc cgctgattct tgtcatagta gctcagctgg
     1921 atagagcatc cgccttctaa gcggacggtc gggggttcga atccctcctg tgacgctaaa
     1981 aaaccgttag aaactgaggt ttctaacggt ttttctttgt tttttgtgcc aatattgttt
     2041 gttttcaaag gcaaataaaa taaaaaaaca tccttatcga gtacaattat aaggagcaaa
     2101 aaaataaaac tcatcagaaa agaggaagaa gatatgacga tactatctag aattttagtg
     2161 actctagttg caattgaatt tttttatatc atgtatatcg aaacggtgag aactgattct
     2221 gacacgacaa gtagggtttt taaaatgagt aaagaagagt taagcagaaa atctgttcaa
     2281 acattgttta agaaccaagg agtgtacaat ggactattag gagtgggact actttatgga
     2341 gcttatcttt cttccgcatc taaagaaatc acaagtatgc tattaatatc tatttttttt
     2401 gttgcattat atggcagttt gactagcgat cgttggattt tactcaaaca aggtgggcta
     2461 gctctgttgg cattgctttc tattttctta taaatataga aaaaataaga atttatctaa
     2521 caggaaactt tgtgaaaaat attactttat ctcatgaaat atgatattct aatgaatggt
     2581 attttagaaa atttcatgaa aaaggagtta gataggtgtt caaaaattat gtttcctcac
     2641 taaaaaaaga attttcaggt tatgatcttt caagatttca gaaagatctt ttggctggga
     2701 tcacagtagc tgctgtagca ttgcctttag ccttggcttt tggggtatca agcggagctg
     2761 atgcggccgc aggtttgatt acggctatca ttgccggtat cgttattggc ggtttatcag
     2821 gcggttttta tcaaatatca ggtccgacag gtgcgatggc agctatcttg atgtctattg
     2881 cggcaacaaa gggaatgcaa ggtgtattgc ttgctacttt tttagcgggt ctatttttac
     2941 ttgcagcagg acttttccga ttaggaacac tgacttcttt tatcccagca cccgtaatta
     3001 cggggtttac ttcaggtatt gctatcatta ttgcattagg tcagatcgat aatttatttg
     3061 gtgttcactc ggaaggtaca aacgttattg aaaaactaag tagttatcaa acgataggat
     3121 ttccgataaa cacagcctct ttgatcatgg gagggctagt cattttaggg atgtttatct
     3181 atccaaaaaa atgggcgaaa cgaatgccta gttcgctgct ggctatcatt ttggcaacag
     3241 cagttatgct attgttccat ttaccagtag caactgtggg taagattcca caaacattga
     3301 tcagtagtaa tcgtttgaat atgggagatt ttagcttttc tgccttgcaa caagtggcag
     3361 ttccagctat tagcattgct ttattaggaa tgattgaaag cttgctatgt ggtgcttcgg
     3421 ctggccgaat ggcaaataag cccttagata gcaatcaaga attaatggcg caaggaattg
     3481 gaaatctgtt gcttcctttc tttggcggga ttcctgctac tgcggccatc gcccgtacaa
     3541 gcgtggccat caaatctggt gcacaaacta gattagctgg tatgatacat gcagttgtct
     3601 tactgctctc catgcttgtt tttgcgccag tgatgtcaca gattccaatg ccagcacttg
     3661 caggtgtttt gattgtgacg gcttggcgta tgaatgaatg ggaaacgatc aaggagctat
     3721 ttactaataa atattggtca gcagttctat tgtttatcct tacgatgatt tgtacagtta
     3781 tctttgattt aagtattgct atcgtgatcg gggtgatcgg cggctgtgta ttttttgttg
     3841 tcaaaagcgc tgctatcacg atttcattag aaacgatcga ttggcaaaga atggggctgc
     3901 cagaaacgga aaagctgaat aactggacag tagtgtatat tagtggtcca ctgtttttta
     3961 tgtcttcaga aaaactcaaa aataaattgg aagaactaaa tcatcacgat ggtgtcattt
     4021 tctccatgcg aggcgtacca agtattgatt tgacggcacg tgcagttttt gatgaatttc
     4081 aagaaaaagc agagcaaaga ggacaaacga ttatctatac ttccttgcag ccagaagtag
     4141 aaaaacaact ttctcacttg tgggaacata cattcacgca tcaacatcaa acagttgctc
     4201 atgcgttgac agctttacat gcgaaatata ctgcaattag ccattaaaac aatgaaaaag
     4261 aacagacaag actgtaaact gaagatttta tggtcttgtc tgttcttttt aatattgctg
     4321 taaaagtgaa gctagtactt gccagtcttg atggataaca ggtacaaggg agcgattgta
     4381 gaaaatagct gctggatgaa aaagtggaag aattcgatag tttttttctg accattcgta
     4441 ggcatccttt tgttctgtta attgtaagac aggccctgta tagagttgac catgattttt
     4501 ggtaaccgta tattctttgc caagtaaacg ttgaagacca atgtttccaa gtgtcgcaat
     4561 taattttggc tggaccttca tcagttcgta atccaatatt ggtgcatgcg caaatacttc
     4621 ggaacgagta ggtgtccgat ttggataaac agtttctgac tgttgtgtcc gtttattgat
     4681 ccgatgagtc acgcgatacg ggcggctcct tacagcactg gtgatataca cctcttctcg
     4741 tgttaagcca gttgaagaaa gtgcctgcat caattctttt ccagcttgac cgctaaaagg
     4801 aacatgcgta tcaatctctg ttttccctgg ggcttctccc acgagcatca gtttaggatg
     4861 aagaggtcct tgcccagcga taaatccttc tacaggacgg ccgttcattc ttaaagaagc
     4921 ttcttgtatc agttcttttg ggtattccat ttatgctcct cctttttctt ttattataag
     4981 aataaaagaa acacaatgcg actattttat ttggtagaac tgtgaggata gaaaagcaga
     5041 agtattgctt gaatttttgt tttacttttt gattttaaat ttagttgact aacggttgct
     5101 gttctggtaa gctagtactg ttgtaattgt ggactccaca gctacaaccg ctcagaacaa
     5161 gtaataagca cagcatatgc tgtcacttcg gatggcgagt ctaagtatat cagaaggagg
     5221 tgctttatag catgtacgca attattaaaa ctggtggtaa acaagtaaaa gttgaagtgg
     5281 gacaagcaat ctatgttgaa aaacttgacg ttgaagcagg agaaaaagtt gtttttgacg
     5341 aagttatctt agtaggcgga gaatctacaa aagtaggcgc tccaactgtt gcaggtgcaa
     5401 ctgttgaagg tactgttgaa aaacacggca aacaaaagaa agtcgttact ttcaaataca
     5461 aacctaaaaa acattcacac cgtaaacaag gtcatcgtca accatataca aaagtggtta
     5521 tcgacgcgat caacgcttaa tagcgtttga gaaagaaggc ctatatatga tcaaaggaac
     5581 atttaagcga aatgacgcag gtcagatcgt ttcattcact ttgaccgggc atgctgatgc
     5641 tggtccttat ggaagtgata tcgtttgtgc gggtgtttct gcgttagcga tcagcactgt
     5701 caacgggatt gcctcattag ccggttttga gccgattgtt gaaatgaatg aagaagaagg
     5761 cggttatctt tataccgaag tcacttcagg aatgacacaa gaacaaaaca atattgctga
     5821 gattctatta gaaaatcttt tattaggatt acaatcgata gaagctgaaa actcagaata
     5881 tatccaaatt aaaacaatta atgataaata ggaggtgcag accatgttgt tatctatgaa
     5941 tctacaatta tttgcccaca aaaaaggtgg cggttctaca tctaacggcc gtgactcaga
     6001 atcaaaacgt ctaggtgcta aacgtgctga tggacaaaca gttacaggtg gatcaattct
     6061 ttaccgtcaa cgcggtacaa aaatctatcc aggtgtaaac gtgggtatcg gtggagacga
     6121 cactttattt gcaaaagttg atggcgtagt acgtttcgaa cgtaaaggcc gtaacaaaaa
     6181 acaagtgtct gtttatccag tagctcaaga agcttaattg aatcttatga ttaaagaggt
     6241 tgtgacaaga cttttgtcac agcctttttt gtttataaaa ataaaagttg tagtggaggc
     6301 tacgaagttt actttgtctt tttacactta atattttttt gccaatttgg aatcaggatg
     6361 ttttgataac tatcgttttc aattgatgga agctatattg tttgctatca gtgaaataat
     6421 tatatggtaa acagtgttct ttttcttgtt tccattataa aaatcgtatc gggatttttc
     6481 gttcttttaa attgaacatt tctatctatc cgcgtgttat caacaaaaca aaatcaggta
     6541 aatagttgac gaaacagaaa taaatacata gtattatgtt ttgaagccta agtacaatag
     6601 tctatagaaa aggagcgata ccattggcaa aacgcattga ccgaatttat atgtatgtca
     6661 aagaaaaaac cgctcattta acaaaggcag aatatgatca aggagtaaca acacaggaaa
     6721 ttgctgaagt gttagggatt cagcgaacaa atagcagtaa agatctgaat cagctagtgc
     6781 gcgaaggaaa actgataaag acagacggac gaccagtacg ttacatatat caaacactcg
     6841 ttacgcacgt aaagccagtt acaaaacatg tagtcagtta caaagaaacg ccggtctctg
     6901 aagaaaaaaa tacactccaa atagatacta aggatatctt tgcaaaaatc attggagcta
     6961 acggcagcat gaaaaattca gtggaacagg caaaagctgc gattttgtat ccgccaagag
     7021 gattaaattg tttgattaca ggacctaccg gatcaggaaa gacttatttt gcacacgcga
     7081 tgtatcattt tgcaaaagcg catcatgtaa tagatgagga aacggaactg attgttttca
     7141 attgtgctga ttatgccaat aaccctgagc ttttgatgag tcatttattt gggtacgcaa
     7201 aaggtgcttt tacaggcgca gatgaagaaa aaatggggat tatcgatcaa gcggatggcg
     7261 gtatgctgtt tttggatgag attcaccggt tgccgcctga aggtcaagaa atgatctttt
     7321 actttatgga tcatggaact tacagtcgtc ttggggaaac aacgaaatcc catgaagcaa
     7381 atgtacggat cgtaggtgcg accacagaag acccaggttc ttctctatta gaaacttttg
     7441 ttcggcgtat cccaatcaat atcaaacttc cggcttttga aaaacgtcct gcaaatgaga
     7501 aagtcgattt agtaaaaatc atgattgccc acgaagcaaa ccgcacgcag cgaaagattt
     7561 ctttgacaga agatgtagca aaagcattga tcggcagtgt gacctatgga aatataggac
     7621 aattgaagtc aaatatccag ttagtatgtg cacgaggatt tctaaatcat atgcaatcac
     7681 ccgaaatctc gatcacaatc gatgatttga cagaagggat tcgtagcgga ttgattcaat
     7741 tagcaagcaa ccgcagtgca atggcagagc tttctaaaat attggagcca aagataactg
     7801 tagcaccaaa tgatacgatc atgaaaatcc aatcggactc ctatgagtta ccttataatc
     7861 tatatgatat catcggagat aaagcagctt tactgaagtc ggatggtttg gatcaagaag
     7921 caatcaatca tttcatttct acagatatca acatccatct gaagtctttc tataaagacc
     7981 atggtttttc ttttaatgca gacagcaaat tagcagaatt tgtagatccg aaaatcatag
     8041 aagtgaccaa tcagatttat gtaatggtca aaagatcttt accatatgag tttcagcaaa
     8101 actttattta tgctatgagc ttgcatatca gttctttttt gaaaaaaatc aacttaggag
     8161 aagaacggca tacgaatgat aacatccgtg agatggcttt tgattttcca gatgaatatg
     8221 ctgttgcgaa agaagtgcgt agttatatcg aaacgtattt tcaagtggag attccagaaa
     8281 gtgaagacta ttacttgacc gttctactcg tctcgttacg ggcaaatcaa gcaagcggca
     8341 gaatcggtgt agtcgttgca gctcacggaa acagcacagc aagcagtatg gtccaagttg
     8401 tccaacagtt gttagatgca gagaacgtac gagcagtaga catgcctttg gatatggatc
     8461 cgaaaacagc attggaccga attgaacgca atgtacaaga agtagatgaa ggaagcgggg
     8521 tcatactctt agtagatatg gggtcccttg cgtcgtttaa ttcgcaaatt caaagagaca
     8581 ctggaattcc agtacgtact gttgatatgg taacaacttc tctagttttg gaaactgtac
     8641 gaaaggtctc agtattaggg acagatctag atatgttgca tgaatcattg aaaaattttc
     8701 gaggttacgc agaaatatct tctgtagaat ctgttcagga agcaaaacct attcataaga
     8761 aaaaagctgt tttggctatc tgcgcatctg gcgaaggtac agcacaaaga atcaaagaat
     8821 tgatcgaacg agcggtaaac aagcgtcaag aaacagaatt agaagttcta gctttgtcga
     8881 ttgtggaagt gaaagaagaa ctgccagtta tccaaaaaga atatcagatc atagctacta
     8941 ctgggattac tgatttgaat atccaagccc catttattcc tttagaacgt tttattgatc
     9001 aaaatatcga agtcattttg gatcagttgc tgatggaatc agaattggag gaaactgaat
     9061 tcatctccct agatgaagag tcagctaaaa atacgtgtgt tgagtttatc tctgataact
     9121 tcatctttat taatggtagt aagttaatcg atccaatgtg gcaattttct acacaaatct
     9181 ctcagacgac gggaatcggc gatgaagaat atggattcaa aatcaaccta gtgatgcaca
     9241 cagcaggaat gatcgaacgt attattcgga atgaaccatt gactgtggaa gaaaacgaat
     9301 tgacaaatac aacgaatgat cctctttata gccaattggc agcctcagtt gttctgttgg
     9361 aagatcagat aaaagtaaaa gtgccgatag aagaaatgta ttatctgcta cgtctagtcc
     9421 ataatcagtt agataaaaaa gaatacactg tgccatgaat acacaaaaac gatacacttt
     9481 tatagtgtat cgtttttgtg tattggttaa gaatccttta aattcaatta gtgtatcgta
     9541 aaaaagttgg cacgcttttt gcttatagta gtgtgtgcta ggaggtggtt gttcacacag
     9601 aaatgatagg ggtttctgtg aagtagtgag gaagaaggag ttttgcagat ggatattcga
     9661 ttggcacgta tagatgatcg tttaatccac gggcaagtcg caacagtttg gtcgaagcaa
     9721 acaggtgttg aacgtatcat tgtgatcagc gatgtagtcg ctcaagataa attgagaaag
     9781 tttctactaa aagaagctgc accgccgggg atcacagcaa atgtaataac cgtgcagaaa
     9841 atgattcaaa tctatcatag tggattatta tcacgagcaa aagtgatgct tttattcacc
     9901 aatccccaag atgttgaagc ggttgtccgc tgcggtgtac agttgaagtc gttgaatatc
     9961 ggcggtatga gttttactga aggcaaatca atgattacca attttgtttc agtaaatcaa
    10021 caagatattg attcctttcg ttacttgaac agtcaagaag tggagttaga gattcgaaaa
    10081 gtacctgctg acaggaaaat ccttttgatg gaactactag cgaaaatcaa aaaggtgtag
    10141 caagaatggt tgttggaaaa ggtcaaataa ttctgttgta atggttagca attattaata
    10201 tttttgaatt tgttacaaca aaagtaaata ggaggtataa atatggtagg gattatccta
    10261 gcaagtcatg gacaatttgc tgaaggaatc ttacaatccg gttcaatgat ctttggcgaa
    10321 caagaaaacg tcaaagccgt gattttgaaa ccaagtgaag gtccagatga cttgagagcc
    10381 aaattggaag aagcggttgc ttcttttgat aatcaagacg aagtgttatt cttagttgat
    10441 ttatggggcg gaacaccgtt caatcaatca aacacattat tcgaagagca caaagataaa
    10501 tgggcaatcg tcagcggttt gaatttacca atgttgattg aagcctacgc atcacgcttt
    10561 tcaatggaat ctgctcatga aattgcagca cacatcattg aaacagcaaa agacggtgta
    10621 aaagtaaaac cggaagaatt agaaccggca gaagcaccga aagcagcagt tgaagacgca
    10681 caacctaaag gagcacttcc agaaggaact gtggtcggag acggaaaaat caaatatgta
    10741 ttagcacgtg ttgactcacg tctgttacat ggacaagttg cgacagcttg gacaaaagca
    10801 gtgcaaccaa accgtattat cgttgtttca gatgcagtat caaaggatga tctgcgtaag
    10861 agactaatcg aacaagctgc tcctccaggt gtaaaagcaa atgttattcc tattagcaaa
    10921 atgatcgaag tagcaaaaga ccctcgtttt ggaaatacca aagcattgct tttatttgaa
    10981 aatcctgaag acgtattgac agctgttgaa ggtggcgtag acatcaaaga attaaatgtt
    11041 gggtcaatgg cgcattcggt cggtaaagtc gttgtaagta aagtattgtc tatgggacaa
    11101 gaagacgttg aagcgtttga gaagcttgaa caaaaaggtg tgaaattcga tgtacgtaaa
    11161 gtacctaatg attcacgcga taacatggat gatattttga aaaaagcaaa agctgaatta
    11221 gcaaaagcgt aataaataat attaaaattt ataggaggct tatcatgtct attatttcaa
    11281 taattttagt cgtattagtt gcctttctag ctggtatgga aggtattctt gatgaatttc
    11341 aatttcatca accactagtg gcatgtacat tgatcggttt agttaccggt aacctcgaag
    11401 ctggtatcgt acttggcggt acgcttcaga tgatcgcttt aggttgggca aatatcggtg
    11461 ccgctgtagc accagatgct gcattagcat cagtagcatc agcaattatt ttagttttag
    11521 gtggacaagg tgttaaaggt gttccttctg cgatcgcgat tgccgttcct ttggctgttg
    11581 cagggttgtt cttaacaatg atcgtacgta caatcgctgt tccaatcgtt catttgatgg
    11641 atgcggctgc tgaagaagga aacatccgta aggttgaaat gtggcacatc attgctgtat
    11701 gtttacaagg ggttcgtatc gcggtcccag cagcagcgtt gctatttatc ccagcacaaa
    11761 ctgtgcaatc attccttgaa tcaatgccag catggttaac agatggtatg gctatcggtg
    11821 ggggaatggt cgtagctgta ggttacgctt tggtaatcaa catgatggct acaaaagaag
    11881 tatggccatt cttcgttatt ggtttcgttg tagctgcaat ttctcaatta acattgattg
    11941 ctctaggtgc tttaggtgtt gcattagctc ttatttactt gaacctttct aaaatgggtg
    12001 gttcatcaaa taatggaggc ggaagcaact ctggtgaccc tttaggcgac attttgaatg
    12061 actattaatc ttgaaggagg aaaataaaat ggcagaagaa aaaatcaaat tatcaaaaaa
    12121 agatcgttta gctgttgcgt ggcgctctac cttcattcaa ggttcatgga actacgaacg
    12181 tatgcaaaac ggtggctggg cattttctat gattccagca atcaaaaaat tatacaaaac
    12241 aaaagaagaa cgttctgcag cattaaaacg tcacttggaa ttctttaata cacacccata
    12301 cattgcttct ccaattttag gggttacatt ggctctggaa gaagaacgtg cgaatggcgc
    12361 gcctgttgat gatgtagcga ttcaaggggt taaagtaggt atgatgggtc ctttagctgg
    12421 tgttggtgac ccagtgttct ggtttacagt acgtccaatg ctaggtgcac taggtgcttc
    12481 attagctatg ggaggaaata tccttggacc aatcatcttc ttcttaggtt ggaacattat
    12541 tcgttgggca ttcatgtggt atacacaaga atttggctac aaagctggtt ctaaaattac
    12601 tgatgacctt tctggcggat tgttacaaga cgtaacaaaa ggtgcttcaa ttttagggat
    12661 gttcgtatta gctgcccttg tacaaagatg ggtatcaatc aaattcttac cagttgtatc
    12721 aactgtaaaa ttagataaag gtgcttacat cgaatgggat aaattgcctt caggtggtga
    12781 aggaatcaag agtgcatttg aacaagttaa taatggctta gccctttctc ctgaaaaggt
    12841 aacaacatta caagacaact tagaccaatt gattcctggt ttagctgcat taggattaac
    12901 attgttatgt atgtggttgc ttaagaaaaa ggtttctcca atcgttatca tccttggttt
    12961 attcgtagtc ggtgttgttg gacacgtcat cggactttta taatccataa aaatatagct
    13021 cgggcgttgt cccgagcttt ttttatatag tatactgttc ataaacaaat atctggaggg
    13081 agaaaatggt tcaatcaatc aatacaaaag tagacttggt aattgatgct acttcgttta
    13141 cgggtctgac agattacggg caaatcatga taggtgataa tggttttgag ttttaccatg
    13201 cgcgagatag acgcaaatat atccaaattc catgggaaga agtggattat gtactagcat
    13261 cagtcatgct aaagggaaaa tggattcctc gatatgccat tcaaacgaaa aaaaatggta
    13321 catacacatt ttcttccaaa gaacctaaaa aagtgcttcg tgcgatcaga gaatatgtag
    13381 atgaagatag aatggtacaa tcattgagtt tctttgatgt agtgaaacgt ggtattcgta
    13441 gtatgtttaa acgcaagtaa aaaataaaga aaacttctaa gaggaggttt tctttatttt
    13501 tttactttgc agaagaaaat atgaaatagc aaaaccaatt atttgtcccc tttaaatccc
    13561 atatgctaaa attgattagc aaagaaggag gttatattat gagttggtta tggacattga
    13621 ttgttggagc cgtgatcgga gcaattgcag gtgcgatcac cagtaaagga aattcgatgg
    13681 gctgtgtaac gaatatcatt gctggactag tcggttcaag tattggacaa gcgattttcg
    13741 gcgaatgggg accaaaagct gcaggtatgg cacttattcc atctatttta ggcgcagtga
    13801 ttttggtagc agttgtctca tttttctttg gaaaacgatc ataagttttt acaaaatagt
    13861 aaactaaaag gagttgcagc gagactgtga ctcctttttg gctttattta tttaaaataa
    13921 aaagaagttt tccggaaatt tttatttaac tgtcgtgtac gaagaaaagt ttgctataat
    13981 ggaaaaaagc gtgcaaagaa agagggagtt tcattatgat gctacgtgta gaaaaattgc
    14041 gcaaaaaaat gcaagaagaa aatttggatt catttttggt taccagtccg tacaatcttc
    14101 gttacttaac gaactttaca ggtacgacag gattagctgt tattactttg gaaaaagcat
    14161 tttttattac tgattttcgt tataccgagc aggcggcagc gcaggctcaa gggtttgaaa
    14221 ttattaaaaa tgtcggacct atttttgatg aggtagcaaa cttagtacgc aaagaagaac
    14281 tgcactcatt agcttttgag gaaacaacag tctcattttt agaatattcg gttttagaag
    14341 aaatcatcga tgcggaatta gtacctgttt cacagatgat cgaagaactc agagaagtga
    14401 aagatgaaga agaaatcgct attatcgaaa aagcatgtca tattgcggat atggcatatg
    14461 atcacatcct gaaaatgatc caaccgggca tgactgagat tgaagtcgca aatcaactag
    14521 acttttatat gcgttctctt ggcgcttcag gtgtttcttt tgatacgatc gttgctagcg
    14581 gattgcgttc ggctatgccg catggtgtag caagcaaaaa aatcatcgaa caaggtgact
    14641 tgatcacatt ggactttggt tgctattacg aagggtacgt ttccgatatg acacgtacat
    14701 tcgctattgg tgatcctggg gaaaaattaa aagaaattta tcaaatagtc ttagatgctc
    14761 aattagcagt tattgatgct gcaaaaccag gaatgactgg tgtccaatta gatgcagttg
    14821 ctagagatta tatttcaaaa catggttacg gggaagcatt tggacattct acaggacatg
    14881 ggatcggttt agagatccac gaaggaccaa acgtttcttc tcgtgcagaa aagcaatttg
    14941 taccaggaaa tgtgatcact gatgaaccag ggatctactt accaggaatc ggtggagtac
    15001 gtattgagga tgacttgttg atcacagaag atggaaatcg tgtgttgact cgttcaccaa
    15061 aagaattgat catcttatag tttcatcaat agaagaagta agcgtaaaaa tcagtgttca
    15121 cagactttgt tatagaaaat cactcgattt aatgatacac tatagaagaa cttttaagct
    15181 gtaagagata ataaagttgg gtgttttctc caattcatag cagtttgcag ctgatcggcg
    15241 gcagttttct taccacgtga tcactatgcc gcatcataga gaattcggat gtatctcgtg
    15301 cttaggcaaa gggaggaata aataaatggc agatgaaaaa aatttgattt taaacgcaaa
    15361 ccaagattta ggagagatcg tgatcgctcc tgaagtcatc gaagtaatta tcgggattgc
    15421 agcttctaaa gtagaaggcg tatacggaat gcgcggaact tttgccagca atgtcactga
    15481 attattagga cgtgcagctc acggaaaagg tgtttactta gcaaacgagg aagatggctt
    15541 gaaagtagat ctttactgtt atctagaata tggtgtttca gttcctaaag tagcaatgga
    15601 aatgcaggaa cgggtcagcc agcaagtcct tttcatgacg gatatcgatt tatctgaagt
    15661 caatatccat gtagtagctg ttgttcctga aaaattagaa caaccaacaa tcgaagaact
    15721 ttttgaagac gaagaggaag agaatgaata aagaattatc aagacacgag attcgggaaa
    15781 tggcattgca ggctttattt ccattagact tcaatgcaga cctaacaaaa gaagatgcga
    15841 ttttcaacgc aatcgaatta gaccaccgcg acatgatcaa cgaagatgaa tcagagtttg
    15901 ttcctgttta tcttgatacg ctagttggcg gtgtctgtgc aaaaaaagat gaattggata
    15961 aagtgattga aaaacattta aaaaacaatt ggcacatcaa tcggatctca aaaatggatt
    16021 tggtgatctt gcgtatcgcg atttttgaaa tgacttatgt gacagatgta ccagcggctg
    16081 ttgctttaaa tgaagcaatc gagttggcaa aaacatttag tgatgaccgt tcacgtaaat
    16141 tcgtcaacgg tgttttatcc aatgtattga aagaattaga agctggggcg taagtcctag
    16201 cttttttcta tgaatcatca taaacctgtt ataaatcggg aaaattctat ggtagaatga
    16261 aaacacaaaa aatatctggg agtggttaga tggcagaatt gattaacgga aaagaattag
    16321 ctgaaaaaat gcaggcagag attgctgaaa aaatccaaaa attgaaagat aatggtatcc
    16381 atcccggatt agtcgtttta ttggtagggg aaaatccagc cagccaaatc tatgtaagaa
    16441 ataaagaacg tgtagcaaaa gaaatcggaa tccattcatt ggtagagcgt tatcctgaga
    16501 cgatttctga agacgaacta cttgcagaaa ttgaaaaata caatcaagat cctagttttc
    16561 atggtatatt ggtccaatta ccattgccaa agcatataga tgaagaaaag atcctgttag
    16621 ctatccgccc tgaaaaagac gtagacggtt ttcatccgat gaatttaggc agactccttg
    16681 cgggtaaacc ggacaagatt ccttgtactc catacggcat catgaaaatg tttgaagctt
    16741 attctatcga tccggcgggt aaacgagcag tagttatcgg acgaagcaat atcgtcggta
    16801 aaccgatggc tcagctactc ttgatggcag atgcaactgt tactgttgct cattctaaaa
    16861 ccgaaaatct ggctgaactg gcacaagaag cagatatttt agtcgtagca ataggacgtg
    16921 gacattttgt cacaaaagaa ttcgtaaaac caggtgctgt agtgatagat gtagggatga
    16981 atcgtgatgc taacggtaag ctgatcggtg atgtgaaatt tgatgaagtg gagcctattg
    17041 caggctggat cactcctgtg ccaaagggtg tagggccaat gactatcacg atgctgatgt
    17101 atcaaaccgt aaaatctgcc gaagcttttg gagcaggtga atagatggca caagaatatt
    17161 taactgtcac tacattaacg aagtatttga aaagaaaatt tgatgccgat ccttatttag
    17221 aacgcgtata tttaacaggc gaaatatcga atttccgttt acgtgccaat gcccatcaat
    17281 atttcagctt aaaagatgac catgcaaaaa tatctgctat tatgttcaaa ggagcattcc
    17341 aaaaattacg attccagcca aaagaaggaa tgaaagtcct tgtcgttggg cgtatttcat
    17401 tatatgaagc gggaggttct tatcaaatct atgtagagca catggagcct gatggaatag
    17461 gtgctttata tcaagaattg gaagaacgaa aagaaaagct gatgaaggaa ggcctttttc
    17521 aagggcctaa aaaaatgctg ccacgtttcc caaagcgaat cgcggtcctt accagtccaa
    17581 gcggagcggt tatccgcgat atcatcacga cggtgaaaag aagatatcca attgtccaat
    17641 tggttttgtt cccaacagtt gtacaaggaa accaagcagc ggacaatgtt gtcaggaata
    17701 tccagagagt agaagagatg ggcaatttcg atacaatgat catcggtcgt gggggtggtt
    17761 cgattgagga tttatggccg tttaatgaag agcgggttgc tcgggcgatt tatcaagcta
    17821 atacgccggt gatttcatct gtaggacatg aaacagatac cacgatcgct gatttagtag
    17881 cagatgttcg ggctgccact ccaacagcag ctgcagaatt agctgttcct gttttgtcag
    17941 aagagttgat gcgcattcaa gaacggcaag ctagattgca gcaggcgttt gtacgccaga
    18001 tccagcgcaa acaagaacgg ttcgagcgtg ctcgacgttc ctatgtattt cggcagcctg
    18061 aaagattgta tgaaggtcag accatcaaat tagaccagtt gaaacagcga ttatatcaag
    18121 ctacacagca aatttatcat gcgaaagaaa aacaaacgat cgcattaagc caccaattgg
    18181 aacaagtggc tccgctttat cgagttaaag cagctcgcca agaaactgct tatctagaaa
    18241 aacgcttaga agagaaaatc caacaatata tgaaacaaca gcagcagcgt tttgctcaag
    18301 ctgttcaatc tcttgatttg ttgagtccat tgaaaatcat gggaagaggc tacagctata
    18361 cgacgttaaa tgatcaagta gtaaaaagtg tcaccgagat tcatcaaggg gatttgctga
    18421 cgatccatta tcaagatgga atcgttaaag gacaagtaca agagatagac aaaggagaat
    18481 aaaaatatgg aaaatccaac ttttgaagag tcactgcaag aattagaaaa aattgtcatg
    18541 cagttagaac aaggagacgt tccattagaa tcagctctag attcgtttaa acgaggaatg
    18601 gaattaagca aacattgcca agatacatta gctaaagcag aagagacctt gacgaaaatg
    18661 atgacagaat caaatgaaga agttgatttt gacggaaacg aggaaagctg agatgagatt
    18721 aactgacttt tcagctattc atctacctgc tgtagaaaaa gaaatcttgt cttttttaga
    18781 tgagcataca acggatagag gactctatga tgcgatgagt tattcagtga aagcaggcgg
    18841 gaaaagaata cgtcccttat tacttttaac ggccgttgct tcctttgatg agcctattga
    18901 tgttcccgtc tatcaagtag cagctgcttt agaaatggtg catacgtatt ccttgatcca
    18961 cgatgatttg cctgcgatgg acaatgatga tcttcgtaga ggaaaaccta ccaatcataa
    19021 agtattcggc gaagcattag ctatattggc cggagatggt ttgcttacag gagcattcca
    19081 gctaatcagt atggcacact taggtaattc tcctaagctt ctgctgcttc agcaattagc
    19141 tgtttgtgcg ggatcacaag gtatggttgc tgggcaagct gcagatatcg agggcgaatc
    19201 gaaaaagctt tctttagaag aattagcatt tattcatgaa agaaagacag ggcatttgat
    19261 tcgttatgcc ttgctggctg gtggcatatt agcgaagcaa cctgaagaaa tcttattgct
    19321 acttcaacga ttagccgaac atttaggttt agcttttcag ataagagatg atctactcga
    19381 tgtgattggt acaacgaaaa cattagggaa aacagctggt aaagatgaac gtatggaaaa
    19441 aaatacttac ccgcgtttat taggcctaga aaagacacgt gaggcgttgg agattgagct
    19501 gttatccgcc aacaagatca tagacaaatt agaagaggac gttttttctt ttgatggcgg
    19561 gctgatgcgc cagatgatca agcaatttga tgtggagaaa tgagggaaag aatggaaaaa
    19621 gaacgcgtag atgtattagc ggtcaaacaa ggattatttg aaacaagaga acaagccaaa
    19681 cgttcagtga tggctggttt gatttacaac gaaaaaaacg agcggtttga taaaccggga
    19741 gaaaaaattc cagtgagtag tgaattaaga gtcaaaggta aaaaacttcc ttatgtttct
    19801 cgagggggat taaaattaga aaaagcactc aaagcatttg atctatctgt tgaaggaaaa
    19861 acattgttag atattggtgc atccactggc ggatttactg atgcagcttt gcaaaatggc
    19921 gcaaaaatga gttatgcttt agacgttggc tacaatcagc tagcttggaa aataagacaa
    19981 gatcctagag tcgtcgtaat ggaaagagtg aattttcgtt atgcaaaacc cgaagacttt
    20041 accttaggaa ttccagaagt tgctgttatc gatgtttctt ttatttcact aaaactgatg
    20101 ctgcctccat tacatgctat tttaaaagaa gagggagaag tgattgcttt aatcaaaccc
    20161 caattcgaag caggcagaga agctgtaggg aaaaacggaa ttgttcgtga ccctcagacc
    20221 catcaaaaag ttttagaaga cattttgtca tttgcggcac aaggtggata tgatgtgctg
    20281 gaattaagtt attctccgat cacgggggga gaaggaaata tcgagttctt ggctcacttg
    20341 aggaaagtac ccgaaagcgg aacaatcaat tcagctatta atatggcaga agtggtttca
    20401 aatgcacacg aacagtttga ccataaataa attgtaactg tgaaaatcaa gtgagaagta
    20461 tgcggattct tctttattat accaaggaaa taagctatca ttgattaaga agaatatgct
    20521 aaggagcgaa gacgtaatga gaaaaaaaga tcggcatcgt ttgattactc gattgttgag
    20581 tgagcaagat atccgaaagc aggaagaatt tgttgaaatc ctgaaaagca aaggaatatc
    20641 tgttactcaa gctacgattt ccagagatat aaaggaactg aaactgatca aggtaccggc
    20701 ttcagatggc ggctaccgtt atagcatacc agctgaaaca aacgaagatg taaatactaa
    20761 attggaaaaa ctattaaaag acgctttcgt ggcagttgac caaatggaaa aatttgttat
    20821 tttgaaaaca ttgccgggaa atgcttcggc agcagcaaat ttgattgata aacgttacaa
    20881 gaaagaatta ttttcgatta tcaatgatga cgataatgtt ttgatgatta cacgaactga
    20941 agaccaggca gttttattga agaaagactt cctgcattac ctttaagttg aggagcgaat
    21001 gtgatgctac aagaaatcag tattactaac tttgcgatta ttccggaatt gcggctttct
    21061 tttcacgaag gaatgaccgc gctgaccgga gagactggtg cgggtaaatc cattattatc
    21121 gatgctttag gtctgttagc aggaggaaga ggttcaagcg attatatacg tcaaggagca
    21181 gaaaaatgtg tgcttgaagg gttgtttgaa ctaccaaaac aagaaggatt ctctgaactg
    21241 atggttgaat tagggatcga aacagatgag gacaatctga ttgttcgacg ggatatgtcc
    21301 ttaactggaa aaaatgtttg tcgagtgaat ggtcatatta tcacattagc taatctgaga
    21361 agaatcggca gttatttagt agatatccaa ggacaaaacg aacatcagga actgctccag
    21421 cctgaatcac atcttgcttt attggatcgt tttggagatg tagctttcca acagaagaaa
    21481 aagagttatc agcaagaata tatctcttat cgtgagctgg aaaaaagggt acgaaaagtg
    21541 cagcaaaatg aaaaatccta tgttcagaga atagatatgc tccatttcca gcaagaagaa
    21601 atagctgctg ctgatttgca agtcggagaa gaagagaaat taaaagaaga aagagaaaaa
    21661 cttagcaatt atcaaaaaat tgctgatgga ttagctgcag gttacggagc actgacgaat
    21721 aatgaacaaa acagtgtaga tggtgttggc ttggccgttt cagaactgca agggatcgcc
    21781 catctagatg tggagtatga agcaatttat gaaaatatcc aaagtgctta ctatttgctg
    21841 caagatgcaa tcggcgatat gagtcgtcag attgatttat tggagttaga tgagagtcgt
    21901 ttagaagaag ttacgcaacg tttagaatta atccgacagt tgaaacgtaa atacggcgaa
    21961 tccattgagt caatattggc ttactatgac gaaatcaccg aagagctagc ttcttctgat
    22021 ttttcagaag gtcaattgga caaaatgaaa gaggaactag agcaaaaaga gcttctactt
    22081 cagcaacaag ccgcagatct tcatgaagcg cgtaaaaaaa tcgcaaaaga attggaaaaa
    22141 tccattctgc atgaattgaa aagtctgtat atggaaaaca ctgagtttga ggtacgtttc
    22201 ttaaaagaag aaaatagaca attgaatcat gatggatttg accaaatcga attttacatt
    22261 acgaccaatc caggagagcc actgaagcct ttggtgaaag tcgcttctgg tggcgaactg
    22321 tctcggatgt tgcttgcttt gaaaactatt ttttcttctg agcaaggtgt gacaagtatt
    22381 atttttgatg aagtggatac aggtgtgagc ggaagagttg cacaggccat cgcagataaa
    22441 atttctcaaa tatcgaaata ctctcaagtt ttgtgcatca ctcatctgcc tcaagttgct
    22501 gcagtcgccg actatcaata ttatattgtg aaagcagtga ttgatggacg tacgcaaact
    22561 tctgtttcag agttgaaaac aaaagaaaga gaagaagaaa ttgcacgtat gctggctgga
    22621 agtgagatta caaaactcac actcgaacat gcaaaagaac tattgaaact cgcaaaaagg
    22681 acctgagcca cctcgcagtg gttcaggtcc ttttcagcag ctgatagcta tattttattt
    22741 ttacatgaca ctcattacag cagtcaatac aacagatcca atcgttaaaa tcagcattgt
    22801 ccaaatgaca aattttgtta ctttagcaaa agtacttgtt tgtttttttt cacgcattgt
    22861 tttcaacctc gtcattaatt agttgattca agtgtacaat cttttttcca tttccacaag
    22921 agggcttttt tataaaaact tccctttttt taaataatag gtagttacga tacaaataaa
    22981 taatgttaat agaactagcc atataaaggc atcatccctt tcagcaatag gtaacttaat
    23041 gttcattcca tagatactgc cgacgattgt cggtatagtt aaaataatcg ttaaagaagt
    23101 taaaattttc ataatattat tcagattatt cgatacgatt gctgaaaaag tctgattcat
    23161 ttgttctact agttttaatt gaatgcgaga agtagtcatt gcctgctctg tttccaccaa
    23221 tacatcacgt aaatgatttt tcaaagcatg attttcttgg aaaagtttat tttgcgctag
    23281 ttctttcaat gcatctaaat tagcagaagt cgcttctctg aaataaacga ggcttttttg
    23341 gatatccatg atttgataaa gttgtttgtt ttcagtggat acttgaagtt tggcttccaa
    23401 agtatcgcat tgagattgaa gcgtagctaa ataactgttg taagagagtg tgacctgcca
    23461 aatgaattga aagaagatat tgagattagg cgaaatatca ctttttggaa aggtctggct
    23521 aaagactttc tttaaaaaca gtggttcatg attcgtaatt gtgataagct tctttttggg
    23581 cgtcaaaata atagagagcg ggtatgtatc cacttgaaaa taaccgctag gacttgtttt
    23641 agcaaaagga tattgtaaca ataataacgc tggctttgtc aactttgatt gattgaattc
    23701 ttctgtacgg gaattttctg catcgtccaa tatggaagtg atgtaatctg acggaatttc
    23761 aaattgatgt gtcagctgat gaatctcttc ttgattagga cgttctacac aataccagat
    23821 cgtttggtca gtttcctttc cggtattacg cagtttctca ttatctaatg taaaatattt
    23881 gatcatgatt ttcctcctgt tgcctgtttc ttttattata acggataggg aattggaata
    23941 catggaatgt gtttgtaaga tatagaatat aactgcataa atttaaataa aatgaaacag
    24001 ttgtgtaaag tatatacagt atacttgaag caacgataaa aaaggagttt acgttcatgg
    24061 gcacattcac accacttatc ttgttttggt atatttaccc ggtaatcgtt ttgtttggtt
    24121 gccaatttct tgtaaaatta ttttcgttaa atcgtcgttt taaaataaaa gcccctgatt
    24181 tggcgattcc ttttttgtgg ggcggtatgc atgcattgtc tagaaataca gggacaattt
    24241 ctatcttacc gtttttcttt atttctgttt tgctaatggg aatttgtatt gctgttttcc
    24301 aagcatatta ttatgaagaa attatttatc cgcgttattt caagatgact tggcgtttgg
    24361 tttttctgtt gacaatcgtc ttgtacgctg tcttgatcat tgtgaacata ctttcttacc
    24421 tatgaaaaaa atagaaaagc tgtaaaaagt cttttaaaag gctttttgca gcttgatttt
    24481 taaaaaaatg tttatgaata aaaaaagttg ttcccggatt tcccagaaaa ggtggtagaa
    24541 agtgggggat tgtggtagac taattttagt tagtggagga atggggggct ttcaatgttg
    24601 atgggtgaat tccaacataa tattgatgca aaaggacgtt taatcgtgcc ctcaaaactt
    24661 cgtgaagaac ttggcgaaaa gtttgttctg acacgcggat tggatggctg tcttttcggc
    24721 tatccgatga gcgaatggga aaatctggaa gctaaactga acgaaatgcc gttggctaaa
    24781 aaagacgcac gtacttttgt gcgattcttc tattcggctg ccactgaatg cgaacttgat
    24841 aaacaaggac gaatcaatat acccagcacg ttaagaaatt atgcagcttt gacaaaaggt
    24901 tgcgtagtca tcggtgtatc taaccgaatt gaaatttggg atgagacacg atggcaagat
    24961 ttttctgcag cagcagaaga gaactttgac gaaattgctg aaagtatgat tgatttcgga
    25021 ctataagaat aggaagaggc tgactaatga ctgaaacatt tcaacactat acagtcatgt
    25081 tgaaagaaac ggtagatgga ttgaacatca aaccggatgg cgtttatgta gactgtacac
    25141 tcggcggtgc cggacatagt gaatatttat taagccaact aagttctaaa gggcatctgt
    25201 atgcgtttga ccaagatcaa aaagcaatcg atcacgccaa gctgcgttta gcttcttatg
    25261 tcgaacaagg tatggtcaca tttatcaaag cgaacttcag agaattagaa gaacgcttga
    25321 gtgagcatgt agataaggta gacggcattt tgtatgatct aggggtttcc tctcctcagc
    25381 tagatgaagc agaacgtgga tttagttatc atcaagatgc accgttggat atgcgcatgg
    25441 atcagtcagc tcctttatcc gcttatcacg taattaatga atattcctac catgaattag
    25501 taaagatttt cttccgctat ggcgaggaga aattttccaa acaaattgct cgtgaaatcg
    25561 aacgagtacg aaaaacaaaa ccaatccaaa caactggtga gcttgtcgaa atcattaaac
    25621 aagtcatccc agctcccgca agaagaaaag gcgggcatcc tgcaaaaaga atttttcaag
    25681 ctgtgcggat tgcagtaaat gacgaactcg gtgccgaaga ggcctcttta gaacaggcaa
    25741 tccggttatt gaaagtcaat ggaagaatca gcgtaatcac attccattca ttggaagaca
    25801 gaattgtcaa aagtatgttt aaagaatata gtacggtaca agatctacca ccgggacttc
    25861 ctgtcatacc tgacgaattc caaccagaac tgaaagtcat tactcgtaaa ccaatccttc
    25921 cttcagagga agagctaaca gaaaacaatc gatcacgcag tgcaaagtta cgtattgcag
    25981 aaaaaataag aatgaaagag gagtagaatg atggcagaat taaagaaaat gcaagattac
    26041 gaatatgatt tacctgaatt ggtggatcag ccggaacaac aaatgcctga ttcttcttcg
    26101 aatcaagaaa tcattcttcc acaatcaccg gcaaaacgat taaagcagat ttctgctttg
    26161 gaaaaaattg ctgtggcaag cgtggcgatt gctattgttg ctttatgtgt tttaacagtt
    26221 atgctgcgca caaatatcag cggagctgaa aaagaaatca cgtcaataca aacagaaatc
    26281 aatgataaaa agcaagaaaa aacgagttta gaacaagaaa aaaacgaact ttctcgtact
    26341 gaacggatta aacaaatcgc agaagaaaaa ggattgtcta ttaatgacga caatttaagg
    26401 aaagtgaagt aaatgagtct taaaaataaa attcggcggt ttatggaaaa gaaaaatcta
    26461 aatccgatga acaatcgcaa aaaagtaggt atcattttat ttgctacgag tattggattg
    26521 ttctttttat ttgccgtccg tttctcgtat atcgttattg gaggtcatgt tgctggtacc
    26581 tcattagctg aaaaaacaaa acagttgtac caaggaagtg aagtagtaaa agcaaaaaga
    26641 ggaacgattt atgatcgtaa cggtgtggcg ctcgcagaag atgcctcctc ttattcaatt
    26701 aaagcgatac tctcaaaaac atatacctct ggggataaaa aactgtatgt tgaagaaaag
    26761 aattttgata aaatagcaga aatcctgcat aagaatcttt ctatagacaa aaaagatgca
    26821 ctgaatattt tagaggacgg cgcaaaaaaa gagctgtatc aagttgaatt tggtagctat
    26881 gggaaaaata tctcccaaga aaccaagcaa aacatcgaag cggacatgaa aaaagaaggt
    26941 gtcgctggtc tttattttgt cgaccatcag gctcggatgt atccaaatgg cgtcttttct
    27001 tctcatttta ttggatacgc agttccagac aaagatgaga atggcttagt tggtaaacta
    27061 ggtttggagt ctgcttacaa tgatatcttg agcggtaaag atggtaagat tatttatcaa
    27121 aaagataatt ttcaaaatcc tctgccaggt acagtggcag aagaagaaaa agctgtcgac
    27181 ggacaagata tctacacgac tttagacagc agactacaaa gttatttaga aacactgatg
    27241 gatcaagtaa atgaagaata tcagccagaa gaattgacag cagtcctgat gaaagcaaaa
    27301 accggagaaa tcttggcaat gggacaacga cctactttca atcctgaaac aatggaagga
    27361 ttgactggaa aagatgctat ctggcgtaat ttcttggtac aagatagtta tgaaccagga
    27421 tcaaccatga aagttttcac tactgcagct gctatcgaag aaggagaatt caacgagaat
    27481 gaaaccttcc aatctggaaa aatccaagta gaagatgcta cgatcaatga ccatgacttt
    27541 ggtgaaaaag gtgtgctgac catgcgtcaa gctctttcat ggtcaagtaa cgtcgggatg
    27601 gtcatactgg aacagcgatt aggcggacga tggtataact atttacaaaa actgggattt
    27661 ggacaaagta ctcattctgg attagatgat gaagtaaacg gtgctttacc tacattaaat
    27721 attgtcgacc gagcaatgag tgcttatgga caagcggtag gtgtgacgaa tttccaaatg
    27781 atgaaagctt tcacttctat tgcgaataac ggtacaatga ttcaacctcg atatatcagc
    27841 aaagtggtgg atccccaaac tggagaagag cgaacaactc aaacagaagt tctagggcaa
    27901 cctttttcaa aagaaacaac agaaaaagtc cgtgaatata tgagagatgt tgtcgaaagc
    27961 gaaaactacg gtagtgctta tggtgtatat agtgtacctg gatacaatgt atcggcaaaa
    28021 aacggaacag cccaaatcgc ttctgataca gggggatatc aaacaggaga tacagcctac
    28081 ctgtattcga ttgtggagat ggttccttct gaagatccag actatgtctt gtatctaaca
    28141 atgaaacacc ctaagacata tgatcgaatg gcattggcta aaatcgcaaa tccattgatg
    28201 aaacgggcaa tggattttaa agaaactgaa gaagattcag atacagaaac aaaaacagaa
    28261 aaagtatccg tagccgatta tcgtaatctt gaagcggacg ttgcagcagc tgatgctcaa
    28321 aaaagtggac tgcaacctgt agtgatcggt aatggtaaaa aagtacaaaa acaatctact
    28381 gccaacggtg atcagttgat ctctggagaa aaactgatcc tatatactgg tggtgataag
    28441 ctgatgcctg acgtaacagg atggtcaaaa gcagatatca tgaagctggg gaaaatcctc
    28501 ggtattgaag ttagttttga tggagatggt tattgtgtca aacaagaact tgctccatat
    28561 gaaaaaatca ctgaagacaa acttagtttt actttagaag aataagaaga gaaataggag
    28621 agaaaagagc atggaatgga cacaagcatt gattcccatt gtcagcagtt gtgcaatgac
    28681 aattgccgca atgccgctat ttattggtta ttttcaaatg aaaaagcaag gtcaggcgat
    28741 tcgtgaggag ggaccaaagt ggcacaatag caaagccgga actcctacaa tgggcggctt
    28801 agtgtttctg atcggctcta ttttgacagg aatttgggtt ggagcttggc aaaaacagct
    28861 aactcctact cttttcatct tgctttttgt attagcgtta tacggagtaa tcggctttct
    28921 tgatgatttt ataaaaatat tcaagaaaag aaacatggga ctgaactcga agcagaaatt
    28981 gattggacag attctagggg gaatcatctt ttatcttgtt taccgttctg aaggttatcc
    29041 aggtgtattg aatttcttcg gactagatct acctttagga atcatttatg gtattttcgc
    29101 tctcttctgg cttgttggat tttcaaatgc agtaaatctg acagatggca tcgatggatt
    29161 agttgctggc ttaggaagta tctcttttgc agcttacggt ctaattgctt ggcatcagca
    29221 tcagtatgat gtattaatca tttgcttaag tgttttagga ggtttactgg gtttcttcgt
    29281 ctacaacaga aaacctgcca agatttttat gggagatgtc ggttcattag cgttgggcgg
    29341 acttttagcg gcaatttcta taatgctaaa ccaagaatgg acattgcttt tgattggttt
    29401 gatctatgtt atggaaacag caagtgtgat gcttcaagtt acttccttta agcttacagg
    29461 aaaacgaatt tttaaaatgt ctccaattca tcatcacttt gaaatgtgcg gatggtcgga
    29521 gtggaagatt gatacagttt tttggttgat cagcattgtg acttcgttga tcacattatg
    29581 gatcgtttgg taggagatga cgggaaatga aaaaaatatc aacatatgaa aataagaaag
    29641 tgcttgtctt aggactcgca aaaagcggag taagtgcggc caaattatta catgaattgg
    29701 gcgcacttgt caccgttaat gatggaaaac cttttgacga aaatccggaa gcacaagagc
    29761 tgctttcttt agggataaaa gtaataacag gtagtcatcc aatcgaattg ttggatgaag
    29821 aattttcatt gatggtcaaa aatccaggga ttccctacag tcatccttta gtagcaaaag
    29881 cccaagaaat gggtattccg gtaattacgg aagttgagct agcttacgaa gtagcggaat
    29941 gcccaattat tgggattaca ggaacgaacg gaaaaacaac gacaacaacg atgacaggac
    30001 tactactgaa tgcaggagca gatcaaggaa ttgcacgttt agcgggtaat attgggtatc
    30061 cagcaagtgg tgtagcgcaa gaagcaaaat cagaagataa aattgtgatg gaattgtcta
    30121 gcttccaact catgggaatc acagatttcc gccctcatat agcggtgatt accaatatct
    30181 atgaagcaca tattgattat catggtacaa gaaaagaata tgtcaaagct aaatggaacc
    30241 ttcaaaaaaa catgacagaa aaagattatc tgatccttaa ctggaatcag tcagaactac
    30301 aagaattagc gcaaagaaca aaagcacgcg tactgccttt ctcgacaaaa gaagtgttgg
    30361 aagacggtgt gtatgctgac gattactcaa tatactataa aaaagaaaaa ataatggaaa
    30421 tatctgaact tggcgtacca ggtaagcata atgtagaaaa tgcactagct gctatttctg
    30481 tagcaaaatt gtatggtata tcaaatgaag ctatacgaga aacgttgcat tttttccatg
    30541 gcgtaccaca tcgtacgcaa tatgtaggag aaatccaagg cagaaaattt tataatgatt
    30601 caaaagcaac caatatttta gcgactaaaa tggctctaag tggatttgaa acgtctaagg
    30661 tcgtgttatt ggctggcgga ttagacagag gaaatacatt tgatgaatta attccttcat
    30721 taaaaggaat caaagcaatg gtcgtatttg gccaaacaaa agaaaagttg atggatgctg
    30781 gaaaaaaagc aggcatcgag acaatagtaa cagccgattc tgtagaacaa gcagttcctt
    30841 tagcattaga aaacagtaca gatggtgatg tagttttatt atcaccagcg aatgccagct
    30901 gggatcaata cccaaatttt gaaactagag gaaacagatt catggaagct gtgaaccgat
    30961 taaaatagaa aaagtagaag cgagcgataa aaaatgaaaa ttttggttac aggcggagga
    31021 acagggggac atatttatcc agctcttgcc tttgtcaatt atgtaaagac tcaagaaccg
    31081 aatgctgaat tcatgtatgt gggagcaaaa cgaggtctag aaaacaaaat cgtccctgat
    31141 acaggaatgc ctttccatac cttggagatt caaggattta aacgaaaaat ttccatgcat
    31201 aacatcaaga cggtccgatt gtttcttaag agtatccgtc aagcaaaaaa aatcctcagt
    31261 gattttcaac cagatatcgt tatcggtact ggaggatatg tttccggagc ggtagtgtac
    31321 gctgcagcca aaatgggtat tccaacagtt atccatgaac aaaacagtgt accaggtgtt
    31381 accaataaat ttttaagccg atatgtggat cggatcgctc tttcatttaa agatgcggct
    31441 tccttttttc ctgaaaacaa agcagttctt gtaggtaatc ctcgagcaca agaagtagca
    31501 gatactaaaa aatcagatat tttgaaaaca tttgatttgg atccagaaaa gaaaacagtc
    31561 ttgatttttg gcggtagtca aggagcactt aaaatcaatc aagcagtatc agttttttta
    31621 agtgaatata ttccttcttc ttaccaagtt ttgtatgctt caggagatag atattatgaa
    31681 gagatcctaa ataaaataaa tcctgaaata caaaatatta gtgtccagcc ttatatcaaa
    31741 aatatggcag aggtcatggc ttccagtgat ctgctggtgg ggcgtgctgg tgcaacttcg
    31801 atcgcagaat tcacagctct ggggttaccg gcagtgttgg ttcccagccc atatgttacg
    31861 aatgatcatc agactaaaaa tgcaatgagt cttgttcacg caggagcagc aaaaatgatt
    31921 gcagataatg aactaacagg tgaatcacta agtcagacag tcaacgaaat catgggtgat
    31981 gaagaattgc aaaagcaaat gtgccgagct tctaaagaac aaggaatccc tgatgcttca
    32041 aaacgtcttt atgacttagt aaaacaaata atcaaaaaat aaaggcggtg gtcttagtga
    32101 gtaagaaaaa agaaccaaaa aaattgtctc atccgtcttc tgatgaaaac aatttgactc
    32161 cttggcaaaa ggctaaccga aaatatctgg cagaacaaga gatgaaaaac caagagccaa
    32221 aaaaagaaga cataacatta gatacacccc tttcagattc tgaagaagaa aaaacggttc
    32281 aagaggaaca gcaagaagaa agccagtccg agaaaaaaat cgaatttttt gaagagatca
    32341 agcaaaagaa aaacggtgga ccatataacg gttctttttt gaaccggctg cccaatttaa
    32401 aaagtcaacg gcacaaagta ctatttcgtc ggttagcttt gatcatcact gtattgggta
    32461 taccgctgat ttttttgatc tattatgttt ctccttatag caaattacaa gcagttgcag
    32521 tatcaggcaa taagacagtc aacagccaag aaatcatctc tgatacaaaa ttgtcattag
    32581 gagaaaatgt ttggggacag tattttcatc gatctactta tatcgatcgc cttaaaaaag
    32641 cacagccaag aatcgaaaca gcaaatatac atttcaaagg gatgaatgaa ttcgatttgg
    32701 atgtaacaga atataaagag attgctttga tcgcaaaaaa caatcaatac tacccagtga
    32761 ttgaaaatgg aacagtatta gatgaaaaag tagcaaatcc tacaaaaaat ctccctatct
    32821 tggaagagtt caaagactct gcaaaaatca aagaattaac aaagcaatac aatcagttat
    32881 catcagaatt acaaaaagca atttctgaaa tcaagtacac gccaagagca agcaataaaa
    32941 atctgatcca attgaatatg aatgacggca atcaagttat tgtaaatatc aataatcttg
    33001 cgaaccaaat gaaatattat tcgcaagtag ccaaagatat ggatgaaaaa ggtgtgatcg
    33061 acatggaagt tggtatcttc tcgcatcctt acggtagcac tcaagaaacg gaagaatcgg
    33121 gccaatcgga agaaacaact gaaagtgaag aaaatgcgga tcaaacaaac gaatcaagtg
    33181 aagaggcaga aacgcaaaaa gcacaaacac ctggatcgtc tagcacaaac ggatcatctg
    33241 attcaaatga ttcaagtgaa acaagtcaaa tagaagataa tccaagtaca actccgtcaa
    33301 gtttaaatga aaattgataa aacaacttta aaaagtaaga atgtactttc taaaaaccag
    33361 taatttatgg tagaattgga agcagtgaat atatctatta ataagtatat tttaaaacgg
    33421 acgctagttg ttcgcttgta tatggaatag taagtaggag ggaatcccat tcatggcaaa
    33481 aacaggaatg tatgtaggcc ttgatattgg aacgacatct gttaaagttg tcgtggctga
    33541 atatatcgac agccaaatga atattattgg agtaggaaac gcaaaatcag aagggattaa
    33601 ccgaggcatt atcgttgata tcgataaaac ggtccaagcg atacaacgag cagtacgaca
    33661 agcagaagaa aaagcaggta tccagatcaa aggcgtaagt gtcggtttgc cagcaaacat
    33721 gcttgaagta gaaaattgcc agggaatgat tgcagttaac ggagattcaa aggaaatcac
    33781 cgatgaagat gtcagaaatg tggcttcagc tgcccttgtt cgttcaatac ctcctgaacg
    33841 tcaaatcgtg tcaatattgc cacaagattt tactgtcgat gggttcgagg gaatcaaaga
    33901 tcctcgtgga atgatcggcg taagattaga aatgtacggt ctgttgttta caggacctaa
    33961 aacgatcatc cataacatac gtaaatgcgt ggaaaatgcc ggattaatcg tcaacgaaat
    34021 ggtgattact cctttagcgc tgactgaatc aattctttct gacggagaaa aagattttgg
    34081 tacgatcgtc attgatatgg gtggcggcca aacaacaacg gctgttatgc atgataagca
    34141 attgaagttt accaatcttg accaagaagg tggcgaattt gtcactaaag atatctctat
    34201 tgtattaaat acgtcattta acaatgcaga agcgttgaaa ataaactacg gggatgcata
    34261 cccagaacgg acatcaccag atgaagaatt tccagtagat gtgattggtc agtcagaacc
    34321 agtgaaagtc gatgaacgct acttgtcaga aattatttca gctcgaatgg aacagatttt
    34381 caacaaagca aaagatgcat tggaccaaat cgaagcatta gagttgccag gtggcgtcgt
    34441 attgacaggc ggagctgcta gtcttcctgg tgttgtggac ttggcacaag aaatcttcgg
    34501 cgtaaatgta aaattgtatg ttccaaatca catggggctg agaaacccgg tatttacaaa
    34561 tgtaataagt atcgtggatt attcagcaaa cttgagtgaa gtgtatcaat tggcaaaaat
    34621 tgctgttacg ggtgaaacct cagcagctcg tcaaatggta gttgaacaag aaacaacaaa
    34681 cacatatgag agctatgaag cacctgaaga aacaatctac gatgagccgg aaccaaaaga
    34741 gtccggagaa aatgtaaaaa ataaaatcaa gggtttcttt acaaatattt ttgattaatt
    34801 catcctggga ggaataagat atggaattct caattgataa taatatcaat gacggcgcag
    34861 taatcaaagt catcggagtc ggtggtggcg gtggaaatgc tgtaaaccga atgattgaag
    34921 aaaacgttaa aggtgttgaa ttcattacag ccaacacaga cgtgcaagca ttaaaaaatt
    34981 caaaagctga aacagttatc caattaggcc ccaaatatac tcgtggttta ggtgctggtt
    35041 ctcagccaga agttggacaa aaagcggcag aagaaagtga acaatcctta cgcgaagctt
    35101 tagacggtgc agatatgatt ttcatcactg caggaatggg tggaggaacc ggtacaggtg
    35161 ctgcaccaat cgttgcaggt atcgcgaaag aattgggcgc attgactgtt ggtgtcgtga
    35221 cacgtccatt cacttttgaa ggaccgaaac gtggccgttt tgctgcagaa ggtatcgctc
    35281 gtttgaaaga aaatgtggat acattattaa ttatctcaaa taaccgttta ttagaagttg
    35341 tagataaaaa gacaccgatg cttgaagctt tccgtgaagc agataatgtt ttacgacaag
    35401 gtgtacaagg tatctctgat ttgatcacag cgccaggtta cgttaacctt gactttgctg
    35461 acgtgaaaac agtgatggaa aaccaaggta cagcattgat ggggatcggt gtggctagtg
    35521 gagaagatcg cgtagtagaa gcaacgaaaa aagcaatctc ctctccattg cttgaaacat
    35581 ctattgatgg agctgaacaa gtacttctta acatcacagg cggtttggat atgacattgt
    35641 tcgaagcaca agatgcttca gatatcgtag caaatgcagc aactggcgat gtaaatatca
    35701 ttctaggtac atctatcaat gaagaaatgg gcgatgaaat ccgtgtgacg gtcatcgcaa
    35761 caggtatcga tgaatcaaaa aaggaacgca agtctagccg accagctaga caagcacaaa
    35821 tgcaatcacc tgctcaaaag actgtattag atatggatca agctaaacca acttcttcag
    35881 aagaagaaaa cagttttggc gattgggata tcagacgtga acaaaatgtt cgtcctagag
    35941 ttgatgattc caattttgac aatatcgaaa aaaaagaatt tgatacattc aaccgcgaag
    36001 aaacaaaatc tcatggcgat gatgaattaa atacgccacc gtttttccgt cgtaaaaaat
    36061 aaaggagagg aagagcacat gattgctgac aacttgcaga aaatcaagca agaaattcag
    36121 gattcgtgtg ctcttgttca tcgtgatccg aaagaagtta cattagtagc tgtaacaaaa
    36181 tctgttgaat ccgctcaagc tgaagaattg gttagagctg gcgttatgaa ttgtgcagaa
    36241 aaccgagtag ataaattatt agaaaagaaa caagatttat ttgattttcc tgaaataaag
    36301 tggcatttaa ttggtaattt acagcgcaga aaggtaaaat tgattgtaaa tgaaattgat
    36361 tactttcatg cgttggacag tttaagatta gcagaagaaa tccagaagcg gttagagaaa
    36421 gaattatcct gttttattga agtaaatgtt tctggtgagg aatcaaaaca tggaatttca
    36481 ccagatgagc ttttaccgtt tgtggaatct ttagctgcct gcacaaaaat caaggttgtt
    36541 gggttgatga ctatggcgcc taaagaagct tctgatgagc aaatccataa agtgtttggt
    36601 acgctgaaag aattgcgtga tcttgttcaa gcaaaaggtt atgcccatgc accatgtagt
    36661 gaattaagta tggggatgag tcaagacttt caaattgcga ttgaagaagg tgcaacattt
    36721 atccgtgtag gaacagcgct attcaaagac gaataaagga ggaacaaaaa tgccgctttt
    36781 aaacagagga gcaattgcta attttttcgg attagctgat gaggaagagt acgaatacca
    36841 agattatcct gaaacaacaa aaaaggctgt acagccaact aaaaaaaata caacacagaa
    36901 tcaatcaact cattctgtat ctgctgcgaa tactcatgaa aaacaaaaaa cggcgacttc
    36961 acgtcctgta acgcatgaaa gtattcagcc ggctgaaaag aaaaaagtaa ttgaactaca
    37021 tcaatcacct acaaaaactg tagcgaaaga aagaacagct tcaagaacct ctacacaacc
    37081 taaagcaaat cttgctggaa aaatcactgt aatggaacca agagcttatt ctgaagctat
    37141 ggcgattgct aaacgaatca ttgctggaga agcaacctta gtgaattttc atttagtaga
    37201 agaaaaacaa gcgagaagaa tcgttgattt tctaacagga acagtttatg cactagatgg
    37261 agatatcaaa cgtgttggag atgagatttt tctttgtaca ccgactggga tggagattga
    37321 cagttcaacc gctcaatctt ttgcagatgc gaatttgttt gatctgtaag aggaggaact
    37381 acgatttata ctattgcact attacttcat ttacttcaac gggcagccta tttctatagc
    37441 attatcttga tggtatatgc attgctctct tggtttccag gcgggtatca atcagggcta
    37501 ggacgattat tagctaagat ctgcgaaccg tatatcagtt tgttcgatcg tctgcctttg
    37561 aggattggac ccttggattt cacaatttta ggggcaatcc tgatcttgaa cttagccgta
    37621 caaggtttag cacagatcgt tatatggata actgtccatt tagtttattg attgaagcaa
    37681 caaaatcttt attctcagaa tagataggag gggaaaattt tggatgcaaa tgtataccag
    37741 cactttcggg cagatgaacg tacatttatc gattcggtag gcgattggat tgaacaggtt
    37801 caaggacaat atgcacccta tctgaccgaa tttcttgacc caagacaatc ctatatttta
    37861 gaaacactga ttcgacaaag cagtgatttg cgtttcatgt tttatggcgg atatgaacag
    37921 gcagaaagaa aaagatgttt gattttcccc gattattacg aacctgtaga ggaagatttt
    37981 gaaacagcat tgtatgaaat ccattatcct agcaaatttg ccagcctaag tcatgggaaa
    38041 atccttggaa ctttagtggg aacggggatc aaacgctctt actttggcga tatcatctcg
    38101 gacggtgatc gctggcaagt atttattgca aatgaggtgg atcattttgt tgctgcacaa
    38161 gtaacaaaga ttggaaaggt tgctgtacgc ttggaaccaa tcaaatacac agaaatgatt
    38221 atgccgaaag atagctggac acaagaaaga ggaacagtta gttccatgcg actagacagt
    38281 gtgatctctg agatttataa tatttccaga cagcggtcca agcagctgat cgaagctggt
    38341 aaagtaaaag tgaactggac agaaaataca agacctgatt ttgtacttga actattagat
    38401 attatttcag tcagaggtta tggcagagtc cagatccaag aacttgaggg gaagacgaaa
    38461 aaagaaaagt acagggtact gttcggtgta ttacgtaaat aaaataaaaa ggtggtttac
    38521 tcatggcttt aactccatta gatatccaaa ataagacatt cccgacaaaa atgcggggat
    38581 ataatcaaga cgaagtagat gatttcttag atttagttgt tcgtgactat gaggaattga
    38641 ctcaaagaaa tcgagaatta gaaaaagcag tcaaacattc tgaagaaaaa cttgagtact
    38701 tcaatgaatt aaaagatgct ttgaaccaat caatcatcgt agctcaagat acggctgata
    38761 aagtaaaaac aagtgcaagc aaggaatccg aagtgatcgt tacttctgca caaaacaaag
    38821 cagatgagtt agttgcgaac gctgaaaaac gtgcacatca attaacaact gatgcagaag
    38881 aaaaagcaag aaaaatcttg acagatgcaa cagaaaaagc aagacagctt gcgacagaaa
    38941 ctgaagatct taagaaaaaa acacgagtat tccatcaacg tatcagcttg atgcttgagt
    39001 ctcaattaga acaagtgaaa agtccggaat gggatgaaat tttacaacca ttctccagct
    39061 acgtgactga cagccatgaa gtgatcaaag aagttttagc aaaacagctt gacaatgaaa
    39121 atgatactga tgtaaactca gacacaggag tcatagaagc acctatgacg gcatttgata
    39181 cacaggctat tgatatctcg atcgtgcctg gtgaaaatga aaacgagtag aacagaaaag
    39241 cactttgtta ttcttaaagc gagccgatga gagtggaagt tcggtaagac actgcatggt
    39301 gaaagatcct ctacttgaga cctgcttttc cgaaattaga gtaagaagca gcggctgacc
    39361 tcgttattgg tcacagagag tttgcactag atagatgcaa ctaaatttgg gtggtaccac
    39421 ggcatgttcg tcccttgcgg ctgaacatgc ttttttgtgt ccggaaaaca aataaaggag
    39481 tgtatggttg aatgaaaatg aaagaaacct tgcaattagg aaaaacaaaa ttccctatgc
    39541 gtggtaattt gccaaaccga gaaggcgagt ggcaaaaaga atgggaagaa aatcgaatct
    39601 accaaaaaag acaagagttg aacgaaggaa aacctagctt tgttcttcat gatggacctc
    39661 catatgccaa tggtaatatc catttaggac attcattgaa taaaatcagt aaagacatta
    39721 tcattcgttc aaaatcaatg tctggctatc gtgcaccata tgttccagga tgggatacac
    39781 atggattgcc gattgaacaa gtattggcta ataaaggcgt gaaacgtaaa gaaatgtcca
    39841 tggcagaata cttggaaaaa tgtcgtgaat atgcactttc ccaagtagat aagcaacgag
    39901 cagatttcaa acgcctagga gttgcaggag attgggaaca tccttatgtt acattagatc
    39961 cttcttatga agctgcacaa gtacgcgtat ttggtaaaat ggctgaaaaa ggctatattt
    40021 acaaaggatt gaaaccaatt tactggtctc cgtcaagtga gtcttcttta gcggaagctg
    40081 aaattgaata taaagatgta aaatctccat ctatttttgt agctttcaat gtcaaagacg
    40141 gcaaagatat tcttgatgaa gatacagctt ttgtcatttg gacgacaaca ccatggacat
    40201 tgccagcaaa ccaaggtatt gctgtaaatc caaaatttac gtatgtattg gtggaagccg
    40261 atggccgtaa gtttgtcgta gcgaaagatt tgctagaaac agtccaatca gaaatcggtt
    40321 gggaaaatgt aacagttcta aaagaatttg ctggacaaga aatggaatat atgactgcaa
    40381 gtcatccatt ctatgaacgt gaatcacttg tgattttagg cgatcatgtt actttagacg
    40441 ctggtactgg attagtccat actgctccag gacatgggga agatgactat atcgctggta
    40501 gcaaatataa actatctgta gtgtcacctg tagactcaaa aggattgttt acagaagaag
    40561 cacctggatt tgaaggtatc ttttatgaca aagcaaatcc tatgatcact gatctattaa
    40621 aagaaaaagg agctttgctg aagctagatt tctttactca tagttatcct cacgattggc
    40681 ggacgaaaaa gcctgtgatc tatcgggcaa ctccacaatg gtttgcatct atcgataaat
    40741 tccgtcagaa tattttggat gaagtagaaa aagtagagtg ggttatccca tggggtaaaa
    40801 cacgtttgta taatatgatc cgtgaccgcg gcgattgggt aatttcccgt cagcgtgcat
    40861 ggggagtacc acttccgatt ttctatgctg aaaatggaga agcaatcatc acaccagaaa
    40921 caatcgaaca tgtggcaaaa ttatttgctg aacatggatc aattatctgg tttgaaaaag
    40981 aagcgaaaga acttcttcct gaaggattta cccacccagg ttctcctaat ggcgaattta
    41041 caaaagaaaa agatatcatg gatgtctggt ttgattctgg ttcttctcat gaagctgttt
    41101 tacgtcagcg accagaatta tcattcccag cagatatgta tttagaagga tcagaccaat
    41161 atcgtggctg gttcaactca agtatcacga ctagtgtggc tatcaatggt gtagcaccat
    41221 acaaatcagt tatttctcaa ggatttgctt tagatggtga aggccgtaag atgagtaaat
    41281 ctttaggaaa catcattgca ccagaaaaag tgatcaaaca atttggtgca gatatccttc
    41341 gcttatgggt atctagtgta gattatgaag cagatgttcg tgtttctatg gatatcttaa
    41401 gtcaagtggc agaagtatat cgtaaaatca gaaatacgat gcgtttctta ttagcgaata
    41461 cagaagattt cgatcctaaa aaagatactg tttcgtacaa tgatttacgt tctgtagaca
    41521 aatatatgac cgttcgattg aaccaagtaa tcaaagaaat ccgagaagaa gggtacgaca
    41581 aatataactt catgcatatt taccgtacag ttatgaactt cttgacggtt gatctatcgt
    41641 cattctacct ggattttgct aaagatgtag tttatattga agcagaagat gattatcaac
    41701 gtcgttgcat gcaaactgtt ttctatcaaa cagctgtagc tttgacaaaa ttattgactc
    41761 ctatcattcc gcatacagca gaagaaatct ggtcattcct gaaagaagaa gaggagtacg
    41821 ttcagttaag cgagtttcct ggttatgaag agtttgcaaa ccaagaagaa ttgatggata
    41881 cctggactgc tttcatggac ttccgtgata atgtcttaaa agcattggaa gaagcaagaa
    41941 acagcaaatt gattggtaaa tctttggaag caaaactaac tgtttatcca aaacaacaag
    42001 ttcgtgaaat gttaaaagct ttggatgcag atattgcaca attattgatc gtttcaccag
    42061 actactttga agttatggca gcagatgaac aagtgccaga taacgctatg gtatttgatg
    42121 atgttgctat tacagtggaa aaagcagacg gggaaacttg cgatcgctgt cgccaagttc
    42181 gtaaagatgt aggagtagat gaaaaacttc ctcatctatg cggtcgttgt gcaaaaatcg
    42241 ttgaagactt ctatcctgaa gctgtagctg aaggattcga agaaaagtaa aagggaaaag
    42301 ggggtgtgac atgttctttg tcacaccccc ttttgttatc aatcgtttaa ttttccacgt
    42361 tcacgatacc agttatctgg ttgccagatc cattcgaatc cgtctttttc aagtagttcg
    42421 aaagctgctt taggtcccat tgatccagct ttgtagttgg gaaaatccgg ttgtgtttgg
    42481 tcccaagctt ttcgaatgac atccacgata tgccaagatt gagcgacttc ttcccaatga
    42541 gagaagttcg ttccatcacc attcaacgca tctaatagta atttttcgta agcttctggg
    42601 ctattttcaa ccatttctgc actatttcta aattccaatt tgattggttc cgtttcaaat
    42661 ccttgcccta cttctttccc attcaaggat aaagaaaatc cttcagttgg ctgaatatag
    42721 atcgttagaa cattaggtgg aagtgtcgta tcgtcacatg gttcatcgac agatgttttg
    42781 aatacattaa caggaacttg tttgaatacg atgttgatcc gagtcccttt ttcagtcaaa
    42841 cgtttgcctg tacgtacata gaaaggaaca cctgaccaac ggaagttatc gatagtgaat
    42901 ttgcctgcga cgaatgtttc agtctctgat tgagcatcca ctttgtcttc ttcgcgatag
    42961 cccttgaatt gtacaccatc caattcacct tgagcatatt gcccgcgaac gaagttttct
    43021 aacacttctt cttcgttata aatgcggata gctttcaatg ctttgatctt ttctgtacgg
    43081 atttcttttt cggaaaatgc tactggcggt tccattgcaa gtaatgctac aacctgtaaa
    43141 atatgatttt gcaccatgtc tttaagtgcg ccactatgat cgtagtagcc cccgcgatct
    43201 tctacaccga ggttttcgcc aaaagtaatt tggatattgt cgatatagcg attgttccat
    43261 tgagcctcaa aaatattatt ggcaaaacgg atagctgaga tgttttggat catttctttt
    43321 cctaaataat gatcaatcct gaaaatatct tgttctggga atacttctct gatttgctgg
    43381 ttcaattcaa aagcagactg gtaatcagaa ccgaacggtt tttctatgat caaacgattg
    43441 aagccatttt ttgtcataat attttgtgat ttcaaatgag aaacgatcgt tccaaaaaat
    43501 tgaggtgcca tagccaaata atagacacgg ttaccgttta attgatagtg tccatctaat
    43561 cggtcagaca attcttttaa tgtctgataa tgcgttgaat catttacatc atgggattga
    43621 tagtaaaaat ggcttgcaaa ggtagttgct tcttcatctg aaggattcaa tgcacggatt
    43681 gtttctttga caacttcacg gtagtgctca tcgctccagg gacggcgagc ggttccgatt
    43741 acagcaaaat gctcgcctaa atttcctttt ctgtaaaggc ggaacaaaga cgggtacaat
    43801 ttacgctggg ctaggtcacc tgtaccgccg aatatcgtaa acaatacatt tttttcatta
    43861 gacatgtgga tacactcctt tatattttat cgacaaaaat cgtgctggcc gccttaaagc
    43921 tgacggggat tttctttcta tcattttgga tagtgatcgg tccttcataa gctgcgatgt
    43981 caacgatctc atattcttcg ttgatattca ggtcaatagt gactaagtaa tccaacaatt
    44041 ctttttcatc aagcactcgt gcgatacgca cttttgtacc gacaggatag gactccaatg
    44101 tttgtcggcg ttcttcatgg acaacttcct cttttttagg aatcgatcct ccatgaggac
    44161 aatgttcagg atgattcaaa tagttttcta aatgatctgc aagatgttcc gaagtcacat
    44221 gttccaatac ttcagcatca tcatggactt cattccagct atagtctaaa tgttcaacta
    44281 aaaaaacttc ccataagcga tgtttgcgaa tcagactgct agctttttgc agtccgcttg
    44341 cagtcaattg tacaccttga taaggggaat gttctacaag ctcttctttc acaagtttag
    44401 aaatcatttc actgacagat gcagctgaga catctagact agatacgatt tgtttattgt
    44461 tgactttcgt tgtatctcca cctagttcta aaatgatttt caaataatct tctcgatttg
    44521 gtgtcatatt cgatcatcct ttcatggtaa aaaaccatca ttagtaagtg taacaaaaaa
    44581 aacgttaatt gactattttt ctaaataaaa aacaaaggat ttttagacgt ttttacaaca
    44641 aaaaacgaaa gatgatagag aagactcatt ctttcgttta taaaggatat atatatacat
    44701 tgggagggat ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa
    44761 gagtttgttc gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac
    44821 gatattcatc tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt
    44881 aatcagatgt aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt
    44941 gatactttga gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac
    45001 ttgagagaac ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac
    45061 tcctttatga gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa
    45121 taaaagctaa aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta
    45181 tgataggata catagtagga agaaaggtga cataatttga agtatcagca agtggaaata
    45241 gcttttttta ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa
    45301 ataattattg cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct
    45361 gaagtggcta ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct
    45421 gtaaaaaaga agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt
    45481 gatggtattt tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac
    45541 aagcgtgaag taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga
    45601 caaaaagcat ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt
    45661 cctgatgcgc ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa
    45721 gatggttatg ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg
    45781 atccatgaag aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga
    45841 gttcaaatcc tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc
    45901 attccatttg atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata
    45961 atgatcaact taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct
    46021 ttaagcacaa aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag
    46081 gaatttggtt ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc
    46141 aatattactc atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa
    46201 gctaagcaag caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg
    46261 ccaaaagaaa tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa
    46321 gcagcaagaa atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa
    46381 gacgagataa tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta
    46441 ttagatggag aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat
    46501 ttaggtgttt accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat
    46561 tatttcccaa gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat
    46621 gagaattttg atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag
    46681 atttattttg actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa
    46741 tttcatgatc gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca
    46801 atggcagatg aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg
    46861 ggattgttgt atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat
    46921 ttaagaaaaa atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg
    46981 aatttgtaca agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct
    47041 caccttctct tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag
    47101 cggtttctcc tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg
    47161 tggaaccagg tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc
    47221 aagtagcaga tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg
    47281 tgggcggagc caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga
    47341 ttggtacacc aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag
    47401 tgaaaacaat ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca
    47461 ttgatcaaat tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag
    47521 cagatcgttc ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg
    47581 tgacagcaga ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac
    47641 gaaagaaaga tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt
    47701 tctttaatca actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac
    47761 cggtaggaag tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat
    47821 tcaaaaagaa aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata
    47881 ttacaggtct tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc
    47941 atcgttcggg aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag
    48001 ataataatct aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga
    48061 tttttttata cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg
    48121 cagtaaataa aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca
    48181 aagaaaaaat aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa
    48241 ggcgcaaata agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca
    48301 gaggaggggt tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca
    48361 acaaggattg acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag
    48421 ccgatattta agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt
    48481 cgtttactct ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac
    48541 gcaagtgact tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca
    48601 ggcaaaagct gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac
    48661 tggtacaggg aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat
    48721 gctttctgca gatgcgccat ttgtttcttt caattgtgcg gattatgctc aaaatccgca
    48781 actgctcttt ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga
    48841 tagtcccggt ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg
    48901 tttaccgccg gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc
    48961 gcttggagaa agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga
    49021 atcttcagaa agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc
    49081 gaatcttgct tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca
    49141 agaagcgaat cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat
    49201 gctttatgat gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc
    49261 aaaatctttt ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga
    49321 actttccctt caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga
    49381 ccgattcatg gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt
    49441 ctggtcacaa gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac
    49501 ttctctttcc gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt
    49561 ggatgcttat ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct
    49621 tttgccagat gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt
    49681 agaccgtatt tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt
    49741 ggatcgtgtg agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa
    49801 ttttaagagg gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca
    49861 agttgagatt ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg
    49921 agaaagtgag cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca
    49981 gactgcttca agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc
    50041 ttttaatatg ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt
    50101 caaaaactat caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt
    50161 aaacaccttc agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat
    50221 gacaagtacc atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga
    50281 agatatttat caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc
    50341 ggaaaagtct gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga
    50401 aggagtagct gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga
    50461 actgatccag atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt
    50521 ggaagaatat gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc
    50581 atttttttca gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc
    50641 tgatgatgag atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt
    50701 cttttctatt gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga
    50761 tttgtcgatt atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat
    50821 gttagacgga ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc
    50881 aaaaatttat cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa
    50941 atatcaggta aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa
    51001 taaaataaaa aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca
    51061 ctataaaaaa gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg
    51121 cttcttgcgt tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa
    51181 caatcatgtt agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa
    51241 aagcagcaga agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag
    51301 ataataattt agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca
    51361 tgaaagctca atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata
    51421 tgcaagacta tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga
    51481 aataaatcaa gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc
    51541 catcatgggg ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc
    51601 ggctgctaaa atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc
    51661 acttatacaa gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca
    51721 tatgcaagtg acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt
    51781 tactgattta gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca
    51841 acttcttttt tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg
    51901 agaactctct ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt
    51961 tggatggaga aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt
    52021 gctttgcgtg atgcttttat cggcacactg cccgcaacaa tggcgggatc agtcgctgta
    52081 atgatcaatg caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt
    52141 ttagcagcag acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt
    52201 gtctggaacg gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat
    52261 cttgcccgag cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg
    52321 attcaaggta tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca
    52381 aatgtcgcag atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg
    52441 tcagttggat tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca
    52501 gttatgatca tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt
    52561 acgatcaaaa tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta
    52621 ccagcgacga tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct
    52681 gatgggcaac tgatcattga tttggtgcaa aaatatattg cagagccatt cctaggatta
    52741 tctcaagggt tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt
    52801 ggtattcatg gaccaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa
    52861 ctaatcaata ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg
    52921 ttagatgcaa tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt
    52981 gcatggtttg gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct
    53041 aaacgtgcag attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc
    53101 aacgaaccag tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc
    53161 ttgattgctc cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc
    53221 gctccggtat cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca
    53281 acaggagcgg attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg
    53341 atttgggcac catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa
    53401 taagatgaac gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg
    53461 aggaactgaa atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt
    53521 agaaggatac aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct
    53581 gttctttgaa acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat
    53641 caaagcacag ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt
    53701 ttaaaaagat aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc
    53761 tttgatcgtg ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt
    53821 cacaggtctt tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga
    53881 agaagcaaag gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg
    53941 tttttttacg ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa
    54001 tcagtaacta gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac
    54061 cgggctcgca taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg
    54121 acaacgaaga atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt
    54181 gaggttcatt acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg
    54241 cttttaggaa caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg
    54301 atcgaagcaa cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt
    54361 aagctagaag gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt
    54421 ttgtaggaag gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg
    54481 aggaaatgtt tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag
    54541 aacgttttcg tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc
    54601 ggctaacaag tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc
    54661 taatggccgg cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca
    54721 atcagatcat ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc
    54781 tggcgccatt ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa
    54841 tcagttatca actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa
    54901 agcgtatgga ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg
    54961 aaaaccaacg agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa
    55021 aaggcaatta ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg
    55081 tttatcagct tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg
    55141 ctttttgcag ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat
    55201 attcatgcgg ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg
    55261 taatgatcac accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt
    55321 atccatttaa aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga
    55381 aatggaatga aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag
    55441 tagaacaaac aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat
    55501 tagggtatcg taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat
    55561 atgaagaaac gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt
    55621 gataaaaaac gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt
    55681 tgaaggaaaa tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt
    55741 tgttttaggg ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt
    55801 ttttttagat gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg
    55861 aaaggacctc cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg
    55921 gaaacgtttt gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa
    55981 caaacaagct acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc
    56041 gcctcctaag cgataggtcg ctggttcaat tccagtcagg aacgtacaaa taacccctgt
    56101 atcctttgcg gtacaggggg ttattttgta taagtagaca ttgtagtttc taaactgcta
    56161 tgtcctaaac gtttcgatac gctaagtata tttactcctt gataaagtaa aatagatgca
    56221 tgcgtatgtc ttagtccatc aataaacagt cgaagagaaa atagggaaaa attgattact
    56281 aaagaaaaac ttactaggtc gacggtgtag ttgattttca tacttcaata gattcgaatt
    56341 aaacgatttc gattggctta taccagtcct atccaacgaa agatatatcg ttcttaactc
    56401 tagcggtata acttgaagct ttacgtaaag ccaatgggaa tgaactggag atgacttttt
    56461 attattttta gtaatagctg atgggttaat acttacaact ttccttttca aagtaccata
    56521 taaaaaaacg gtggggttgt ttagcgaaat aaaggatcta agccaatagc ttagatcctt
    56581 tattctatgc tttatattat tttttgcatg tgtgcgaaag tatactcaag cccaatcgcc
    56641 atttcggaaa atcggtactg tagagccatc ttcacggatt ccgtcgatat ccatcttgtc
    56701 agatcctacc atgaaatcta cgtgggtttg acttctattc aatccagctt ctgctaattc
    56761 ttcatcggac atttctgtac cgcctttcac actgaaggca taagcagaac ctagtgctaa
    56821 gtgattcgat gcgttttcat caaataatgt attgaaaaag atgatgccag attgagaaat
    56881 aggtgaaggg tcaggtacaa gtgcgacttc tcctaaacga cgcgcacctt catctgtatc
    56941 aagcagtttt gctaagacat cttctccttg ttcggcagaa aaatcaacaa cttttccatc
    57001 tttaaaagta aatttcatac ccgagatgat ggtgccagca taactcagtg gctttgtact
    57061 tgaaatgtaa ccatctactc gatgactgtc aggtgctgtg aacacttctt ccgtcggcat
    57121 atttgccatg aatttttctc cacgagcatt atagcttcca gcgccttccc aaagatggtt
    57181 tttaggcaat ccgataatga tatctgtacc gggagcagta tagtgaaggg cagaaaattg
    57241 ttctcgattc aattcttctg ctttttttgc taatttttca tcatgcttct tccatgctaa
    57301 gacagggtct tcttcataga cacgagtcgt tttgaaaatc tgatcccata atgcctcaac
    57361 ttgtttttct tctggcagtt ctgggaagac ttttgctgcc cattgtttac cagccgcagc
    57421 cacgacagtc caactgactt tatttgcttg agttgccttg cgtaaattca ttagtgcttt
    57481 gccattcgct gattgataag acgctactcg atggctatct acaccagcaa atgcatcggg
    57541 atcggcagaa acaacgctga tcctgcttgc tcctttttca agccattcat ccgcttgatc
    57601 gattttagat tgtggtacat tctcaatacg atccgttgca gcatgtaaaa gaaattctct
    57661 ttggatctgg tcgtcagtcc attgaacgat aacctcagcg gctcctagtt tataagcttc
    57721 ttgtgtgatc aatcgagcaa ggggtgcctg ttccacgctg atctgtaaaa caactgtgtg
    57781 tcctttttct gtggctacgc cagtttctgc aattaagcgt gcatattttt ttagaagttc
    57841 attaaaatca gataacataa taaaaattcc tcctcaccat tttttctggt aactattagc
    57901 atgataccat ttaattatga ttcaaacaat gaggctttaa ataaaaaaag gaggttattt
    57961 ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg tgaagaaaat
    58021 caaaaactaa ttttgcatat caaaaaaaca tgttacaatg aaattaatca atggtggaag
    58081 cgttataaaa aaattaagga gattaaaaaa tatggcaaga aaaaagacga ttactagaga
    58141 acagattttg gcagctgctt atgaagtagc tgccaccgat ggatttacac gatttactgc
    58201 gagaaacatc gcgaacaaaa tgaattgttc cacgcaaccg atttatcttg agtttaaaaa
    58261 tatggatgac ttgagacaag ctctttttga gcagatctat gatcatttac aatatgaagt
    58321 gttcactgtt gagcatacag ggaatacgat tatcgatctt gcttttaatt atatccattt
    58381 tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg gtggaggaaa
    58441 agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg atccagctta
    58501 tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga tcatagctac
    58561 tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac agattgaaaa
    58621 gatgatccaa gacagtatag atgctatctt gaaattcaaa gaaccaatcg aaatcgattg
    58681 aatatactca aaagttccac aaatcagtag gtaacaagag atgtgtcaaa ataaggaaaa
    58741 gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc atgaatgcaa
    58801 tgaataaaag agccatacag attgaataaa tctggatggc tcttttattg caagtatatt
    58861 aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct tcttcttcag
    58921 cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt gcaaaagcag
    58981 catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca gatccgcaga
    59041 caccgtaaat ttttcccaat agatccaatt cttgcaagtc ttcataaaag tctacgatct
    59101 catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata tctgcctcct
    59161 cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata tccagatttt
    59221 caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg ctagcatata
    59281 cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca attatgaatt
    59341 ataccaaaac atcattgaaa agaaaataaa acctaaagac acctggggaa tgtcatttgg
    59401 ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac gaaaatatga
    59461 ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct ttattagcag
    59521 atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg gatattgagg
    59581 tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt ggctttgaag
    59641 ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga ttccctcgta
    59701 aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat ttgaaaggcg
    59761 gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag atcgaccgat
    59821 tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa gtagggaatc
    59881 ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat ctagcgatcg
    59941 ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca gcagttcctc
    60001 attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc acgatcgaac
    60061 ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg actgcgtata
    60121 caagagacgg cggactttct tgccaatatg aacatacatt ggctattact aaagatggtc
    60181 caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag tcaataggta
    60241 cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct gatgcgcttt
    60301 attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc tgtcgtcgtt
    60361 gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa tattttaccg
    60421 tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat tcctgagacc
    60481 gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc aggaggcttg
    60541 ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa tgctttgcaa
    60601 attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat cgtccgtttc
    60661 ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac tatcgttctt
    60721 gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc cgtgaacttt
    60781 atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt tgtcatcatg
    60841 ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat cattccgggc
    60901 acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat ctatgctagg
    60961 tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt actcatgctt
    61021 tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc agttgttcaa
    61081 gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa atggttcaat
    61141 aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt attgtaaatt
    61201 tatatcagta atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt
    61261 tcttttagta tggttataag attttttctg acaattttat tatcactgat attaacacct
    61321 gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata
    61381 aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg
    61441 actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct
    61501 tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa
    61561 aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt
    61621 gattccttca cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta
    61681 acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg
    61741 ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc
    61801 ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc
    61861 ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta
    61921 ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt
    61981 tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc
    62041 attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac
    62101 cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct
    62161 ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcgta
    62221 ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat
    62281 gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg
    62341 caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa
    62401 ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga
    62461 taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa
    62521 ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat
    62581 ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag
    62641 cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac
    62701 agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct
    62761 taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt
    62821 tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt
    62881 tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt
    62941 tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat
    63001 gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta
    63061 cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact
    63121 gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt
    63181 ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct
    63241 ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac
    63301 gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat
    63361 catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat
    63421 gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt
    63481 gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt
    63541 tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa
    63601 gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc
    63661 tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt
    63721 ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct
    63781 gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac
    63841 cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc
    63901 agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg
    63961 catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg
    64021 ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt tagatagtat
    64081 tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga
    64141 tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg
    64201 gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct
    64261 aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga
    64321 ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag
    64381 cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct
    64441 taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa
    64501 acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct
    64561 gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt
    64621 aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca
    64681 tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc
    64741 cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa
    64801 tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg
    64861 tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat
    64921 gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc
    64981 aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact
    65041 gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg
    65101 agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg
    65161 tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt
    65221 tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca
    65281 agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct
    65341 ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg
    65401 agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga
    65461 acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc
    65521 atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga
    65581 aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt
    65641 gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac
    65701 agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg
    65761 gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat
    65821 tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg
    65881 ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag
    65941 cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg
    66001 gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg
    66061 gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg
    66121 cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg
    66181 atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg
    66241 aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga
    66301 aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg
    66361 taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca
    66421 aagagaactt ggccggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag
    66481 atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag
    66541 aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg
    66601 gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta
    66661 ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg
    66721 aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa
    66781 aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca
    66841 tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc
    66901 aagtaacgag tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc
    66961 gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca
    67021 gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg
    67081 aattgatctt agaaatgatg ggccaaccaa aagacgcata cgaccatgta aacgaccgcg
    67141 cagggcacga tttgcgttat gcaatcgatt cgacaaaatt gcgagaagag ctaggttggc
    67201 agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac
    67261 atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac
    67321 aataatacag aggaaatttc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc
    67381 ctgtctcttt tttaaaataa aaatcaagga gcggataacc atatgttttt attaactgga
    67441 ggaaatggac aattaggtac agaattgcgc catttattag atgaaaaaaa tctgccatat
    67501 gtttcaacag atgcaaaaga cttggatatc acagatgctg agaaaacaat ggcttatatt
    67561 acagaattaa aacccgatgt tattttccat tgcgcagctt acacagcagt agataaagca
    67621 gaagaagaag caaaagaatt agatgagaaa atcaatgtcg atggcacaag aaatgtagca
    67681 cttgcagcaa aagctgtgaa tgcacaattc gtctatatca gtacagacta tgttttcgat
    67741 ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat
    67801 ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc
    67861 atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc
    67921 ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg
    67981 acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt
    68041 tatcatttgt cgaacgaaaa tagctgttct tggtatgagt tcgcaaaaga aatattgaaa
    68101 gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt
    68161 ccgcagtatt cagtcatgag cctgaaaaag acagaagcgt taggttttgt cataccaact
    68221 tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct
    68281 ttttatcaaa tagaatttgg agcatgatat ggaaaaacaa acaagaagtg cacgacgaaa
    68341 agaaaagcca aaagcgaatc aggcagattc gaatttggtg cttgacctgt ttctaaaact
    68401 ccgctatctt attggcgttt tagtcatcgt ggtcatcgtt acttttaatt taaacggaag
    68461 ttcacttggt gtttgggata aatatgtgtc gcaaagagac gatgggaaaa aaactgatgt
    68521 gatttttgga cagaatcggg aagtgcggtc agacgaatgg ctggtgcaga caccttttta
    68581 cctctcacaa gctgaagagg acttttcgtt ggtgaacgag gattattcat taaacggaca
    68641 aaacatgatc attgcgtata attctcctgt aaaggatatt acggttatcg gaaaaccatt
    68701 caactgggga ttctttttcc tcggaagaga tcgaggactt tccttttatt gggcgatgaa
    68761 attagtcgtc atggtcttgc ttggattcga agtactgatg atattaacga agaggaaaaa
    68821 agctctatcg ctaatcggtg catttgcgct gacattttct ccagcagtcc agtggtggtt
    68881 catgcaacat gtgggcgatt tgattttctt tactttggga ctgatgattg ctttttatca
    68941 ttatttctac aatcatgata aaaaatggct acgaacgttg atgatgcttt tggtggttat
    69001 tttcggtatt gggtttattt tagtgattta cccagcccat caagtgatgc tggcgtactt
    69061 gctgattttt tattttgttg gcctgcttat ttactatagc aagaaagttt catgggatct
    69121 gttggatgta gtgttgattg gaggggcagt tttatttatt ggcggagtca tggttcattt
    69181 ctggctaacg tcaaaagacg cattgttagc ttctttgaat accttatatc caggaaaacg
    69241 ggtaagtact ggaggaaagt ggacgattgg cgaatttttt tctttcctta caaattggaa
    69301 aattcctttt gaagatatca actattcgaa taactctgaa gtggccctgt tctatcactt
    69361 ttttccaact gtattcctag caagtccatt tgtgttattt ggcaaaaaag atagcgaaca
    69421 aaagttactg ggacgtattt tgatgatttt ttgtctgttt tctatctttt ggattaccgt
    69481 tgggttgcca aaatggctag cagaaatcac tctgctgtct tatgttcctc cattaagagc
    69541 catccttact tttagttttg cggcttgttt attatcgatc tggtttatcg cttatatttg
    69601 gagagaagat gttattccta tttggtataa attcattttg ttggcagtaa atgcatggtc
    69661 ttatttctat gcgattacgc attcgaaaat ggataattat ttttcggatt ttgaaaccat
    69721 cgcagttgct gtgttgggaa cagtgatttt agcttttgca ttatttggct ggagacgctt
    69781 gttttacctc gattttgtcg ccttagttat cgtatctggc tttttcgtca atccagtcgt
    69841 agaaggtaca ggagcgattt ttgaaaaaac actggcaaaa gaaatccaaa aaatcgatca
    69901 acaagatcct ggtaaagttt ggttgtctga agatgaatta tataacttca ccccaacatt
    69961 gggagtgaaa tccttcaata cagtacgctt ttatccagat atagctgcat ggaagaaaat
    70021 cgatccgaaa gaagaatacg agaagtatta taatcggtat gctcatacaa gagcttttat
    70081 cgctaaggaa gatacaaagt tcattttaga tcgtccagat atgttttcgg tcacgttgaa
    70141 ttttgaagac gcagaaaaat tagggatcga ctatgtgatc agtaaacgac cattaactga
    70201 ttacaaccaa ttatatgaag cgcagttcac gcagctatat ggaccggata aagacggtta
    70261 tatgattttt aaagtagaat atccggatta tccagatgta ccacaaacac aaactcagga
    70321 aaatcagcta gtcgtacagt aataggctag ctgagaataa tcctgaaata ttagtagaaa
    70381 ggagagtgaa gataattcaa gcaatcgtac aaaaatttgt caacaaaatg agtgtcggtt
    70441 tcttgctttt gttgctgacg ctagtctttt ttgtttggac agcaggggta ggttttccag
    70501 caactttgtt tatatataac aatagttcat tacttatcgc ttttagtgtg gctattttgc
    70561 tcattttaaa tgtttataaa gcaaaaggcg tcgattttct ttatctaggt gttgcgcttg
    70621 cccttttcat tttcttttct tactttcggg aattaagaaa tgagtcttta tttggagact
    70681 tactgtttcc tttggttatt ttattttgtt tagtaattaa atggatcgag tggacaaaat
    70741 gggatcgcgg tatttatctg attgtcttta cggtcttcct cggaatcacg ttatatcgtg
    70801 tatttacaga gatccaagta ccagaaggac aaagcatctg ggctccaggc aattcgcttt
    70861 ctgatatttg gatcaatacg aatacaatcg gaagttcttt gatgactctt gctttattga
    70921 tcagtggatt tgccagttct tttgaacgat ggtatgttcg aatcatcggt gggatagcag
    70981 tcgttgcagc ctttttaggt atttgggtct gccaatctag aggtgcatta gtcgcgttga
    71041 ttgtttttgc gatattggat cttttaccta aatcgttgat gcgtgtcatt cgtgcgccat
    71101 ttattgccta cacggttacg attcttttag ctttgccgat ttcttatctg gcggctgtct
    71161 ctgaaaaagt caatttgttt accgatcgag aagatatttg gcataaattc tatcagacgc
    71221 taggagaaaa atctgaacaa gttttactag gaatgaaaac atttatcttc cagcgaggaa
    71281 accaattttt aggaaaccac aatagttata attctatttt gaatatctat ggattgatag
    71341 gatttgggat tgctgctttg ctgcttatcc tttttattgg tcgcttaacg ttgaaagcag
    71401 atttatccaa cgggcagatg acttttatct gggccttttt tgcggtgatg atgcagtcat
    71461 ttatggagga tacattgact tcatacccat gggttccgat tatttatatc ctgttggcta
    71521 tggccgttca tcgatatgat gaaccagaag ttgcggagga attctatgtt atggaagagg
    71581 catatgacga tatgacttat gatgatacat cttatgagga acaaccagat agcgctcatt
    71641 cttccaggat ccaacgctat cattgatagg cacaaaatac gagagaggct gtgacaaccg
    71701 tcgcagcctt ttttggttta ttgcaattgt ctagcatctt ctgtaaaata gaaacgttag
    71761 agaaaatctt gtatcataga aaattaagaa agaaataaga acgcagctcg gtaccaaaaa
    71821 tatggtaaaa tggcacttga ctgtaattca ctgaaaatcg cttggaggaa cacaaatgtt
    71881 taaagatttg tatttattta tgaaggacat ttatcagaac agaaaattat tgctacagtt
    71941 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
    72001 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
    72061 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
    72121 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
    72181 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
    72241 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
    72301 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
    72361 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
    72421 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
    72481 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
    72541 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
    72601 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
    72661 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
    72721 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
    72781 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
    72841 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
    72901 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
    72961 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
    73021 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
    73081 gatatcattg aatttgctga catcggagac catatttatc aacctgtaaa aacttattca
    73141 agcgggatgt tcgttcgtct agcttttgct gtagcaatca atgtgaatcc cgatattttg
    73201 attgtggatg aagcattggc agtaggcgat cttgaatttc aactaaaatg tatggaaaaa
    73261 tttacagaaa tcaagaactc gggaaagacg attttattcg tttctcatga tatcagttcg
    73321 attcgtcgtt tttgtgatcg ttcctattgg ttgaaaaacg gagaagtcgt cgaatatggc
    73381 gatacgatgg aagtaacgac gaattatgaa aactttttaa agaaaaaatc agttaaaaca
    73441 gtggatcgga ataaaaacca agtagaacgg ttcgccgctc cagatatcgt cgacgtgacg
    73501 aaagcggaat tgttggataa agacaaaaat ccggtggata tgctggaaca aggagaaaaa
    73561 ctatacgtta aagtaactta tgaagtaaaa gatgatacaa tcaaaaatcc ggttttagga
    73621 attgcgctac gaacagtcga caatttttat gtatgcggac ttaatactct actagatggt
    73681 atcaagattc cttggaaaaa aggagagaat atcttttatc tggagtatga aaagttaggt
    73741 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
    73801 ttggtatata agacaaagta catgaacttg tttgtcagcg gttcttatat cggagaaggc
    73861 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
    73921 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
    73981 taaagtttta gagtttggtc ccggaaacgg ccgattaacg aaacatttaa tcggagcaaa
    74041 acaatgtgaa gtgagcattg ttgagttaga taaggaattg ttcgattttg tcagcgaatt
    74101 tgctcaagat ggattttatg gtgatatcga aagttttgag tgggcaaact attatgctgg
    74161 acagacattt gattacgtct tgtttgcgga tgttttagaa catttggtag atcctggaaa
    74221 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
    74281 taacttggcg cataattctg tcatgatcga tttgttcaat aaccaattgc cgtgggcttc
    74341 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggct tccaaaaagt
    74401 ttttgaaaaa gcgggattat tcattaatat tgaagattat ttgtacttag ctgtaggtga
    74461 tacagaatta aaatcaactt atgaagaatt gccggaagct gtacgctatg atttcaaaat
    74521 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagcaca
    74581 aagttccatt gcagagccac aaaattctaa ttatgtaaaa gtgttagaag taacccagca
    74641 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
    74701 gacactaagt ttccccgtat cagaaacaac agaaagaatg gtgtttcgct tcgcgcaaca
    74761 acctagcttt atcgaattca gcgcagaagc tgccggagaa aaactaacat ttatcgattc
    74821 gaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aattaccaga
    74881 gttcgttcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
    74941 cggggaattg acaccaacaa tgaaagaatt gttggaaacg attcgtccca tggctgaagt
    75001 cacaaagcag ctttctgaga aaaatgacga attagaaaga gaaaatcatc atcttcttga
    75061 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
    75121 ggaaagtgat gaatggacaa taaaacatag actaggacga cgtaagaaag aaacttcgaa
    75181 aaaaatccaa gaaaaagaac tttctatatg tattgacgag aaaatctggg atgcagaaac
    75241 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc ccttgtccta
    75301 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagaagaagt
    75361 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgagttac gaatcctttg
    75421 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggccaat catggatcat
    75481 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
    75541 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
    75601 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
    75661 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
    75721 gatgttaaga cccaaagcgg tttcaaggta cgtttatcag ggaaaatgag aggaaaagcc
    75781 atcttagact tccaaacagc gagacatcaa aatggcatag ctgtgaaatt aaacggtaaa
    75841 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
    75901 gggataaatt atgcaaggac tcatggcgta aaaaaagcga tccgtcgggt caaattggaa
    75961 ttgaagccag gatcgatcga ctatgcagag tggatcagtc gtcatgagaa accagatttg
    76021 aaagagcaaa ggaaagaagc acaaggattt gcgtaccgtc cattgatttc catacttatg
    76081 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt actggatcaa
    76141 acgtatgatc attgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
    76201 aagtgtctgg aatcctatca agcaaaagat gatagaatca aagtcgtttt ccgaaaagaa
    76261 aatggacaca tcagtctggc aactaattct gcattggaaa tagcagaagg agaatttata
    76321 gcactcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
    76381 ctaaacgtac atccagaact ggatgtgatc tacagtgacg aagataaaat cgatgcagat
    76441 ggcaatcgct ttgatccgca tttcaaagca gactggtcgc ctgatacatt gatgggaaat
    76501 aattatatct ctcacttagg cgtatatcga acaagcattg ttaaagaact tggcggtttc
    76561 cgtaaaggct atgaaggttc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
    76621 cctgcagatc atatttacca cattgaccga gtcttgtatc attggcggac gatcccagga
    76681 tcaactgcaa gtaatggcga agccaaaagt tatatctatg attccggagt gaaagcattg
    76741 acagatgccc tgtctagaag aaacatcaaa ggaagcgtac gtcctggcag aatttctgga
    76801 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagtgtgat cattccaacg
    76861 aagaatggct atgaagatct aaaaacttgt gtggattcga tcatcgaaaa aacgtcttat
    76921 tcaaattatg aaatcattat tgcggataac ggaagtacag atccaaaaat gcaagaatta
    76981 tttgcagaat acaagcatca gctgaaagac cgctttatcg tagaattgat tgacattcca
    77041 ttcaattatt ctcgaattaa caatttagca gcagaaaaag caaacggcaa atacttttta
    77101 ttcttgaata acgatacaga agtgattgag ccagattgga tgacggtgat ggtttcttat
    77161 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
    77221 cagcatgcag gagtattatt aggaatcggt ggtgttgcag ggcatgcatt gaataactat
    77281 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
    77341 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgttaacgg gtttgatgaa
    77401 acattagaag tagcattcaa cgatgttgac ttgtgcttga aagtttatga actgggtcgt
    77461 tacaatgtct atgcacatca agcagaattg taccatttcg aatctaaatc aagaggttat
    77521 gaagatactc cagaaaaaca aagacgtttt gctggtgaaa tcaaaaaaat gcaagataaa
    77581 tggccagcat atattgcgca tgatccattt tacaatgata atttaacaaa agacggaatc
    77641 ggcgactttt cgttgagacc agattaaact ataataaaaa gctgttatgt tgtgattcgc
    77701 tcgcacataa cggcttttta tagttttttg tcaaaaaaag gcagaaggtg ccgactcaaa
    77761 aaaataagtc gaggttggga cagaagtgtt taactccgag aaataagaag aaattcacga
    77821 aaattgcttt tcaaattttt gtgaatttcg gcttatttaa gaagaagttg cttctgctcc
    77881 caccgtttat tcgtttttaa agaatgagac aaaagtgttt cttacttttg tcccacgctc
    77941 ttattcatgc tcagagcgcc aatagataca aaatcatagc caaagtttca cgaaccctag
    78001 ctgtcaaaga atcgatattt tattttaata taggctattt taaaataaat ggtttaaggt
    78061 attcctctac aaaagccact ctatcaatta tcatgggttt aaaataagaa gtgatggaga
    78121 taaccagatt gttcttagcg tcaatataaa gaacatttcc actattacca attgctgaat
    78181 atatttcgtt ctcagaatct aatacccacc ataaatagcc atatctcata ttttgtaagt
    78241 cttctaaagc actggttcta ggtttgaaca tctcgtttat ccacatgggt ggtacgattt
    78301 gttttccttt atacatccct ttatttaggc ataatacacc aatttttgcc agatcacttg
    78361 ccgataaaca caaaccatat cctgccgtcc cgatatttag tggatcacaa aaccagacat
    78421 tggttttagg agacttatcc attgtaaact gcttatgttc ttcagctgtt tcagcataat
    78481 aattgacatg gtttggtatc cctataggat caaatagata cttattggca aaatcaacag
    78541 ttgttagctt acttgtctga tacaacaagc cagttaatat gtgtaaacag acagtttgat
    78601 agttgaattc gccggttata ccttttcttc ctcccaataa atctagtgat gtttttgtcc
    78661 aatcttcact tgaacagact tttgtccatg gatcgccttt acatttgtag ggtgctttca
    78721 ttgttagcaa gtgcttgatt gtaatagcgt aaatagtttt ctccccgcgt ttcactttat
    78781 actctggaaa ataatctaat actttatcat cgatactttt gattaatcct tgctctaaag
    78841 caatgccaat cagcaaagat acaatacttt tcgttgctga catgatatga caagtatcgt
    78901 cttttttata accattccat tcatctgtat aaacttctac cccatcctta tatgcgacaa
    78961 tttgacatat atttggttgt cgaaaaccaa taaaatcatg caactcttgt ttattcataa
    79021 gatattacct cactttcttc tataagcata atgtcattaa aaatcgattt caattttttc
    79081 gattcttcac gtaatacagg tattcttatg gactagacag cgctttggga agatggattc
    79141 tctgaaaaat ataaaggttc actttatgag tggataaaaa aagcagcaaa tacttcgact
    79201 tctttaattt accaacatta gtgaaaatcg attcatctgg agatttgtaa tttagagtcc
    79261 ttgtcttgat tagaggtgta taaagctttt tattagacat ttttaaattc cattacaatt
    79321 tgcttataaa ctaggttttt aaaggtgact atggtaagat aatagagata ttattttgtg
    79381 attaggagtg tatatatgaa taaaaatgga gaatggagtg ctgcacgacg gatattcatt
    79441 atattaatgg atgttattgt atacaatgca gcaatatata gtagtttctt actgaagttt
    79501 cgtggagaga tcccattacg aaatttagaa actttccaac actcagcaat tattatttca
    79561 gtgattttta ttgcgttgaa cattttgttg ggtgcatatg ttttttataa tcgaatgatc
    79621 agtgacatcg tgtttgtcac agtcatcggt caggtattga tgacactagg gatcatggta
    79681 gtgacttttg cgggccgctg gtttgctttt ccacgtcttg ttatcttact ttcatttgtt
    79741 ctcggtacgc ttttcttaag tatctatcgt attctcgtat ataaacttta tttacgcgta
    79801 agtcgagata aaaaggtcgt cattattggg ttagaaaagg atgtcgcacc agcaatccaa
    79861 aattttggtt caaagaaaaa caacaaacat aaagtcgaag cagtcattct tgataactat
    79921 tatgcaaaca tcaaaaatat gattaaagac atcgatattg tctatcttgc ttctcatatc
    79981 gaagaaactg a
//
LOCUS       DASJPC010000003        60875 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJPC010000003 DASJPC010000000
VERSION     DASJPC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.003"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="U4A13_000216"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="U4A13_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN1778548.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="U4A13_000217"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="U4A13_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN1778549.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="U4A13_000218"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="U4A13_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN1778550.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="U4A13_000219"
     CDS             4735..5628
                     /locus_tag="U4A13_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778551.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="U4A13_000220"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="U4A13_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1778552.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="U4A13_000221"
     CDS             7565..8422
                     /locus_tag="U4A13_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN1778553.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="U4A13_000222"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="U4A13_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN1778554.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="U4A13_000223"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="U4A13_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN1778555.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="U4A13_000224"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="U4A13_000224"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1778556.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="U4A13_000225"
     CDS             11178..11936
                     /locus_tag="U4A13_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN1778557.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="U4A13_000226"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="U4A13_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN1778558.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="U4A13_000227"
     CDS             12864..13895
                     /locus_tag="U4A13_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1778559.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="U4A13_000228"
     CDS             13892..14659
                     /locus_tag="U4A13_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN1778560.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="U4A13_000229"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="U4A13_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN1778561.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="U4A13_000230"
     CDS             16624..16944
                     /locus_tag="U4A13_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN1778562.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="U4A13_000231"
     CDS             16944..17294
                     /locus_tag="U4A13_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN1778563.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="U4A13_000232"
     CDS             17461..18399
                     /locus_tag="U4A13_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN1778564.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="U4A13_000233"
     CDS             18414..19244
                     /locus_tag="U4A13_000233"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN1778565.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="U4A13_000234"
     CDS             19288..20313
                     /locus_tag="U4A13_000234"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1778566.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="U4A13_000235"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="U4A13_000235"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1778567.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="U4A13_000236"
     CDS             21391..21831
                     /locus_tag="U4A13_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN1778568.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="U4A13_000237"
     CDS             21834..22436
                     /locus_tag="U4A13_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1778569.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="U4A13_000238"
     CDS             complement(22495..23601)
                     /locus_tag="U4A13_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN1778570.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="U4A13_000239"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="U4A13_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN1778571.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="U4A13_000240"
     CDS             complement(25149..27494)
                     /locus_tag="U4A13_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN1778572.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="U4A13_000241"
     CDS             27773..28675
                     /locus_tag="U4A13_000241"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN1778573.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="U4A13_000242"
     CDS             28888..30663
                     /locus_tag="U4A13_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN1778574.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="U4A13_000243"
     CDS             complement(30729..32315)
                     /locus_tag="U4A13_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN1778575.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="U4A13_000244"
     CDS             32566..32790
                     /locus_tag="U4A13_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN1778576.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="U4A13_000245"
     CDS             32932..34683
                     /locus_tag="U4A13_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1778577.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="U4A13_000246"
     CDS             34683..36467
                     /locus_tag="U4A13_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778578.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="U4A13_000247"
     CDS             36793..37644
                     /locus_tag="U4A13_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1778579.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="U4A13_000248"
     CDS             37698..39449
                     /locus_tag="U4A13_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1778580.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="U4A13_000249"
     CDS             39453..39623
                     /locus_tag="U4A13_000249"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1778581.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="U4A13_000250"
     CDS             complement(39663..40565)
                     /locus_tag="U4A13_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1778582.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="U4A13_000251"
     CDS             40796..42199
                     /locus_tag="U4A13_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1778583.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="U4A13_000252"
     CDS             42199..43632
                     /locus_tag="U4A13_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN1778584.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="U4A13_000253"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="U4A13_000253"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN1778585.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="U4A13_000254"
     CDS             44917..46644
                     /locus_tag="U4A13_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN1778586.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="U4A13_000255"
     CDS             46659..47009
                     /locus_tag="U4A13_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1778587.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="U4A13_000256"
     CDS             complement(47101..47961)
                     /locus_tag="U4A13_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN1778588.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="U4A13_000257"
     CDS             48053..48481
                     /locus_tag="U4A13_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1778589.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="U4A13_000258"
     CDS             48643..48819
                     /locus_tag="U4A13_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN1778590.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="U4A13_000259"
     CDS             48846..49292
                     /locus_tag="U4A13_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN1778591.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="U4A13_000260"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="U4A13_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1778592.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="U4A13_000261"
     CDS             51531..52502
                     /locus_tag="U4A13_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN1778593.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="U4A13_000262"
     CDS             52526..54718
                     /locus_tag="U4A13_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN1778594.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="U4A13_000263"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="U4A13_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN1778595.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="U4A13_000264"
     CDS             55195..55590
                     /locus_tag="U4A13_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN1778596.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="U4A13_000265"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="U4A13_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN1778597.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="U4A13_000266"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="U4A13_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN1778598.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="U4A13_000267"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="U4A13_000267"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN1778599.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="U4A13_000268"
     CDS             58762..60837
                     /locus_tag="U4A13_000268"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN1778600.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DASJPC010000004        60191 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJPC010000004 DASJPC010000000
VERSION     DASJPC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.004"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="U4A13_000269"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="U4A13_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN1778601.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="U4A13_000270"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="U4A13_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN1778602.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="U4A13_000271"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="U4A13_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN1778603.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="U4A13_000272"
     CDS             1640..2272
                     /locus_tag="U4A13_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1778604.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="U4A13_000273"
     CDS             complement(2274..3179)
                     /locus_tag="U4A13_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1778605.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="U4A13_000274"
     CDS             complement(3264..3923)
                     /locus_tag="U4A13_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1778606.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="U4A13_000275"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="U4A13_000275"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN1778607.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="U4A13_000276"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="U4A13_000276"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN1778608.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="U4A13_000277"
     CDS             6166..7251
                     /locus_tag="U4A13_000277"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN1778609.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="U4A13_000278"
     CDS             7248..8312
                     /locus_tag="U4A13_000278"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN1778610.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="U4A13_000279"
     CDS             complement(8561..9364)
                     /locus_tag="U4A13_000279"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN1778611.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="U4A13_000280"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="U4A13_000280"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN1778612.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="U4A13_000281"
     CDS             complement(10242..11249)
                     /locus_tag="U4A13_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1778613.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="U4A13_000282"
     CDS             complement(11256..11900)
                     /locus_tag="U4A13_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN1778614.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="U4A13_000283"
     CDS             complement(12000..12758)
                     /locus_tag="U4A13_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN1778615.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="U4A13_000284"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="U4A13_000284"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN1778616.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="U4A13_000285"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="U4A13_000285"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN1778617.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="U4A13_000286"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="U4A13_000286"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN1778618.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="U4A13_000287"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="U4A13_000287"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN1778619.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="U4A13_000288"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="U4A13_000288"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN1778620.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..19025
                     /locus_tag="U4A13_000289"
     CDS             18156..19025
                     /locus_tag="U4A13_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN1778621.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19151..20050)
                     /locus_tag="U4A13_000290"
     CDS             complement(19151..20050)
                     /locus_tag="U4A13_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778622.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20215..21039)
                     /locus_tag="U4A13_000291"
     CDS             complement(20215..21039)
                     /locus_tag="U4A13_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1778623.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21050..22345)
                     /locus_tag="U4A13_000292"
     CDS             complement(21050..22345)
                     /locus_tag="U4A13_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN1778624.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22361..22840)
                     /locus_tag="U4A13_000293"
     CDS             complement(22361..22840)
                     /locus_tag="U4A13_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN1778625.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22846..23874)
                     /locus_tag="U4A13_000294"
     CDS             complement(22846..23874)
                     /locus_tag="U4A13_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN1778626.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23891..24997)
                     /locus_tag="U4A13_000295"
     CDS             complement(23891..24997)
                     /locus_tag="U4A13_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1778627.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="U4A13_000296"
                     /gene_synonym="adhC"
     CDS             complement(25226..27823)
                     /gene="adhE"
                     /locus_tag="U4A13_000296"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN1778628.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="U4A13_000297"
     CDS             complement(28128..28508)
                     /gene="yajC"
                     /locus_tag="U4A13_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN1778629.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="U4A13_000298"
     CDS             complement(28605..29750)
                     /gene="tgt"
                     /locus_tag="U4A13_000298"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN1778630.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29968..30666
                     /locus_tag="U4A13_000299"
     CDS             29968..30666
                     /locus_tag="U4A13_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1778631.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30834..33473)
                     /locus_tag="U4A13_000300"
     CDS             complement(30834..33473)
                     /locus_tag="U4A13_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1778632.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34092..35123)
                     /gene="queA"
                     /locus_tag="U4A13_000301"
     CDS             complement(34092..35123)
                     /gene="queA"
                     /locus_tag="U4A13_000301"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN1778633.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35316..36491
                     /locus_tag="U4A13_000302"
     CDS             35316..36491
                     /locus_tag="U4A13_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1778634.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36495..37136
                     /locus_tag="U4A13_000303"
     CDS             36495..37136
                     /locus_tag="U4A13_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778635.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37133..38050
                     /locus_tag="U4A13_000304"
     CDS             37133..38050
                     /locus_tag="U4A13_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1778636.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38047..38718
                     /locus_tag="U4A13_000305"
     CDS             38047..38718
                     /locus_tag="U4A13_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778637.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39259..39747)
                     /locus_tag="U4A13_000306"
     CDS             complement(39259..39747)
                     /locus_tag="U4A13_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN1778638.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39948..40214)
                     /locus_tag="U4A13_000307"
     CDS             complement(39948..40214)
                     /locus_tag="U4A13_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1778639.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40214..40477)
                     /locus_tag="U4A13_000308"
     CDS             complement(40214..40477)
                     /locus_tag="U4A13_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1778640.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40953..41138
                     /locus_tag="U4A13_000309"
     CDS             40953..41138
                     /locus_tag="U4A13_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1778641.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41280..43103)
                     /locus_tag="U4A13_000310"
     CDS             complement(41280..43103)
                     /locus_tag="U4A13_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN1778642.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43701..46406)
                     /locus_tag="U4A13_000311"
     CDS             complement(43701..46406)
                     /locus_tag="U4A13_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN1778643.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46712..47659)
                     /locus_tag="U4A13_000312"
     CDS             complement(46712..47659)
                     /locus_tag="U4A13_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN1778644.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47849..48118
                     /gene="rpsN"
                     /locus_tag="U4A13_000313"
     CDS             47849..48118
                     /gene="rpsN"
                     /locus_tag="U4A13_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1778645.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48294..48656)
                     /locus_tag="U4A13_000314"
     CDS             complement(48294..48656)
                     /locus_tag="U4A13_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1778646.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48667..49788)
                     /locus_tag="U4A13_000315"
     CDS             complement(48667..49788)
                     /locus_tag="U4A13_000315"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN1778647.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49804..50154)
                     /locus_tag="U4A13_000316"
     CDS             complement(49804..50154)
                     /locus_tag="U4A13_000316"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN1778648.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50284..51795)
                     /locus_tag="U4A13_000317"
     CDS             complement(50284..51795)
                     /locus_tag="U4A13_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1778649.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52117..53469)
                     /locus_tag="U4A13_000318"
     CDS             complement(52117..53469)
                     /locus_tag="U4A13_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN1778650.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53622..54698)
                     /locus_tag="U4A13_000319"
     CDS             complement(53622..54698)
                     /locus_tag="U4A13_000319"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN1778651.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54894..56639
                     /locus_tag="U4A13_000320"
     CDS             54894..56639
                     /locus_tag="U4A13_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN1778652.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56919..57356)
                     /locus_tag="U4A13_000321"
     CDS             complement(56919..57356)
                     /locus_tag="U4A13_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN1778653.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(57340..58713)
                     /locus_tag="U4A13_000322"
     CDS             complement(57340..58713)
                     /locus_tag="U4A13_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778654.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(59035..59523)
                     /locus_tag="U4A13_000323"
     CDS             complement(59035..59523)
                     /locus_tag="U4A13_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1778655.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59545..60147)
                     /locus_tag="U4A13_000324"
     CDS             complement(59545..60147)
                     /locus_tag="U4A13_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1778656.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17103 a        13016 c        10989 g        19083 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaaattca
    19021 gctgattcat ataatatgta cgtcattttt tcaaaatatt ttgaaatata tagataaaaa
    19081 taaaataacc gcagtttctg cttctatgta aacaacagca gctactgcgg ttatttttta
    19141 gtgatcattc tcattgttca tcttcaaaat gttcataatg aaccaactgt ttctgtgtat
    19201 cacgtttcaa aaattcttta ggcgttatgc cgaactctct cttgaatgct ttcagaaaat
    19261 gcggataaga agaaaaacca cttttactat atacagcact agagctgcgg tattctcgca
    19321 acaagtgctt ggcatagatc agctttttct tcattacata ctgatgaaac gtaaatccag
    19381 tgtacttttt aaattctcgt gtgatatggt atttgctgac aaaaaactgc ttttccattt
    19441 cctctaaaga aagcggatga gataaatttt ttgagatata ttgtaacatt tgttccacac
    19501 gctcattagg aactacagca ttctgttctt gcgcactctc ttctttttgt acaacttgat
    19561 tgagaaaaat caagtattct accagagctt gttcatattt tatgtcttca ccgtaaattt
    19621 ttttatcagg aggatggtcg ataaatgata atttttgctt caatacttcc tgttctattt
    19681 gcaatacacg actcctcttc tctcccattg gttgaaaaca agccgataga ttagaccatg
    19741 gcgtggaacg actttctaga aaagaagggg tgacaaagat atagacacgc tcgaatacat
    19801 ctgaagattg gcggacgata cgatgcaaat cattggaatg gatgagcagc acacttcctg
    19861 ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc ttctcccttt aatgtagcat
    19921 ggatctcata gaaatcatga tgatgataga tcgttttatt ttggctgacg acatctttga
    19981 catgatagat ttcatactga tcagaaacca tcgtattgac ttgttcaccg ctgattttct
    20041 tttggagcat gtgccgcacc tccttttgtt cacttatgca caattttatt ataccacgtt
    20101 ttttgtaatc aaaaagagct gcttttatat actcttataa aaaagaagag acgtggcatc
    20161 ttctagcccc gccccctcca ttctaaatat tagttaacca acgtcttctt tacattattc
    20221 aagaagactt cttccataac gaatcccatc atcttttgtt tcttcaagaa ccacatacag
    20281 gtacggacga ttcttcatga tgatttcttt gatttttgtg tactcgatca caccgtctcc
    20341 caaatttacc ggcacgattt tttcctgttc gacacgaaaa tctttcaaat gaaccgcaca
    20401 tatcttttca ccaaaacgtt caaaagcttc ttctaccaat tggacttgtt cttgataatc
    20461 actacttgtg atcaaattgg tcggatctaa aatgattcct agatagtccg aatctactgc
    20521 ttggatcagc tgctctaccc ttgctaaaga atacaccgga tgattcaaac ctggttctat
    20581 ccccaccatc atttggtatt tttctccagc ttttaccaag cgacggataa cggacacagc
    20641 ttcagcaaat gcttcatctg tgaaattttc ttccgtatat tggatttccg gcaaaacaca
    20701 tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa gcatagcgaa gatactgctc
    20761 gaactttgtc agcaactctt gtcgtatcgt taaatctgga tgaatcatat tgatatagca
    20821 acttaaaatt cctacggtca cttcttgctt ttctaaaact cgtttgacat aatttcccat
    20881 cccagaattg atctcatttg cgccagaagg tatttcagga aacgaaagac ctaaggcgag
    20941 ctgcagtgta tgaatccctt gttcttttgt tttttctgca agatcttcta tcgtttggac
    21001 ttcggaaaga tcatgtcctc taattgtaat gttcaacata actattctcc taactcagca
    21061 tattgggtag ccacatgacg atttgcggga agtaagtaat caaaaacaag acgactaaga
    21121 taaccccata aaaaggaaga agcggtttca gcattgcctc tgcttttacg cctcctacac
    21181 ttgcaccgac atatagtcct gtacctactg gcggcgtgat ggagcctaca cacagattca
    21241 tgattgaaaa caacccgtaa tgtacaggat caacccctac acttgtgacg attggtaaaa
    21301 atattggtgt aaaaatcaag atagcaggcg ccacatccat aaacataccc accagtaata
    21361 acacaagatt gactaataac agaataataa atttattatc cgttacgccc ataatcaacg
    21421 aactaattgc ctgaggaata ccggtaaacg ccatggcgaa agacatcatg gaagaagtag
    21481 ccaataagaa catgatcgtc cctgacattg ctactgattg aaacaacata ttaggtatat
    21541 cttttagttt taccgtttta tagtaaaaca tagcaatcaa cagggagtaa atgacagcta
    21601 tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat accaccgata atgatgacaa
    21661 tcaatagaag gctaggaatc gcctctttta ttatttttcc agcatgagcc atctgtcctt
    21721 tttcgacaac agggtattta ttctttttag caacgatgaa tgctactagc atgattccta
    21781 gcgcccacaa agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac
    21841 cagcaacaag tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag
    21901 ctgacgcgat attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag
    21961 ggatcaacac tccgccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa
    22021 ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca
    22081 acatcgcaaa atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga
    22141 gaacgaaaaa cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag
    22201 ctgaactaaa agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga
    22261 atgcaatact tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata
    22321 acactaatcc ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg
    22381 ctggcttgtt gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc
    22441 ccgatacagg tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct
    22501 gccccattgt caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata
    22561 ccagaaataa actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt
    22621 cacttaaaac cgttacggct aaatgtgctt tttgtcccac aacatacgcg gaagacaaca
    22681 tggcaaacca gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta
    22741 aaaccgttct ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag
    22801 ctcccaggat ctctaatacc ttgtttaaaa tcattttcat cacagctacg ctccttgctc
    22861 ttggatcttt tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg
    22921 ggcaactgcc tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc
    22981 ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt
    23041 tgattctttc gctgcttctt caatggcttt ttcctgctca tcagttagct gttctttgat
    23101 ggcatcattc atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt
    23161 agccacacca ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat
    23221 caaattggat tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc
    23281 taaaagctct accattttga tagcggtttc gctttgcatc acacggatct tctttccctt
    23341 caaatcttcc ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc
    23401 ataataggtc aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat
    23461 ttcttcatta tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaactga
    23521 aaaaatcgaa taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc
    23581 tactgctcct gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg
    23641 gaaatactgg atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt
    23701 cattgcatca attactggct ggctgttgct tgcataagca aagcgcaagg taactatctc
    23761 atttacatct gcatctgcga cagttgccac actagaatta tttccgcagc cgcttagacc
    23821 tagcagacct aggctaagcg aacttgctgc aaacagcagt gatgtttttt tcatatgggt
    23881 ttcctttctt ttaacaataa gagacgagca attctgtcaa acaaagcacc gttaacgact
    23941 gtccataagg cattgctgtg attccgatat tccgataaaa atctaaactg tcccccattc
    24001 ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc gatttgttca agcaatcctt
    24061 ggatagcacg ataagcgact tctttatatt cttgtggtaa ataacgttta tggaccgctt
    24121 tcaaaatacc gtaagcaaat cctgcagttg cagaagattc tacgtaagaa gaaggatcat
    24181 ccaacaacgt atgccataaa ccgctttcag actgataagt ttttaatgca cgcacttgtg
    24241 cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc tttcgataat tctaagattt
    24301 caatgatctc tggaatagca atcgtcaacc agcagtttcc acgagcccaa aatgcttcgg
    24361 catagttatg atttccttca aacgtccagc cgtgatacca caatcctgtt cgtttatcca
    24421 tcaagtattt gatatggatc ataaactggt aacgagcttc ttctaaatat tctgggcgat
    24481 tcaacagttt cccgattttt gccaaaggaa gcactgtcat catcaatgta tcgtcccaca
    24541 actgattctt attttccggt ccatatgtgg catgctgtaa cccgtcttca tttgtccgag
    24601 gcatgtcatt catgacccat tctgcccatt gttctaaata aggcaaatag cgtgaatctt
    24661 tcgtgtcttc atacaagtaa gccatagtca atagcggtgc cattgtattg acgtttttgg
    24721 gcggagcgcc ttcttccata cgatcctcaa accattcatt gaccacttga taagcgcgag
    24781 gattttttgt catctggtaa tttttataaa taccatatag accgacacct tgtggccaat
    24841 tccacactgt ccagcttttg tcatctactt tcagtccatc aaagtccaac aaaaattcac
    24901 cattatcgtc tttgatcgtt gtcaggtttt caacgagcag gtcaattttt tctttcacta
    24961 attctttagg tacaaaaatc tcttctttaa taatcatctt tacgccacct ttttaaacgc
    25021 tttcatttta tatccttaat atagcagtaa taccgtccac tataaatatc gctttttgaa
    25081 caaaaactgt ctaaaattga cctaaatgtt aaataacaaa agtaactttc agttacttgg
    25141 aaattaaaaa aagaacttgg agaaacaacg ttttccaagt tctttttcat ctattttctt
    25201 atcttttttc tacgctttct tttgcttatc cgttgtaagc atcaatgatg atctgtttca
    25261 attcagagat caatggttct ttcggattag ctgtcgtaca ttggtcttcg taagctaatt
    25321 cagccatacg gtctacggtt gtatctagtg tttcttgtgt gactccttga gcttttagac
    25381 tcatgttgat gccgacagat tttcctaatt cataaacagc cgttgctaaa gcttcgacta
    25441 attcctctgt tgtttcgcct ttcagaccta agaattttgc gatatctgcg taatctgtat
    25501 ctgcacggaa atagtcatat ttagggaaca tcgcatgttt ttgtggatct tttgcattat
    25561 agcggataat atgcggtaaa agaatcgcat ttgtccgtcc atgagggatc ccgtattccc
    25621 cgccgatttt atgtgcgact gagtgacaaa tacctaggaa tgcgttagca aacgccatac
    25681 cagccattgt agaagcgttg tgcatttttt cacgtgattc catatcaggt gtttttactg
    25741 atttttctaa atagtcaaag actaacttga ttgcttgtag gctcaatcca cgtgtataat
    25801 ctgaagccat gactgaaaca taggattcga ttgcatgtgt taacacatcc atcccggtat
    25861 ctgctgtgac agatgctgga actgacatga cgaattgagg atcgacgatt gcgacatctg
    25921 gcgtcaatgc ataatctgct aacggatatt tcacatgtgt ttcactgtca gtgatgaccg
    25981 caaatggcgt tacttcagaa ccagtccctg atgttgtcgg gatacatacg aattgtgttt
    26041 tttctggttt gtcgatctta tatgtccgtt tacggatatc caagaatttt tgttttgcac
    26101 caaagaaaga tgtgtctggg tgttcataga acatccacat tcccttagct gcgtccattg
    26161 cagaaccgcc tccaagagcg attactgtat ctggcttgaa gtctgccatc agtttcgttc
    26221 ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc tgagaagact tcgatcttca
    26281 catcattttt acgtcgtgcc aatacttcac gaactgtatc gcagtaaccg aattggacca
    26341 taccagggtc acaaacgatc atgacacgtt caacgttttc cattttctct aagtacaata
    26401 gagagttctt ttcaaagaag atttttggcg gtaatttgaa ccattgcatg ttatttctcc
    26461 gtttcgctac agttttgaca ttgattaagt ttacagcaga gacgttacga gaaacagagt
    26521 tcttaccata agaaccgcat cccaaagtca atgacggaat catttcgtta tagatatcac
    26581 cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg acaagctttc attcgaagac
    26641 cgaatttgac atgcaactct tcgttttctg aatggataac tgctgtatgt cctaatcctc
    26701 caagattcaa cataccttca cacaaatcaa acgcatggtc cgtgttgtta gctttgatca
    26761 tggcaagaac tggagaaagt ttttctcttg atagcggata atctgggcct acgccttcca
    26821 attcagcaat cagcattttc gttccttttg gtacactgat tcctgccaat tttgcgattt
    26881 ccatcgcaga atgtccaacg attgatggat tgaccgcata ttttccttcg ttcatcactg
    26941 catcttctaa tttttgcaat tcatctggtt ttacgatata aacttggtgt gcttggaatt
    27001 ctgcttttac tgctgcatag atttctttat ccacgatcac agcttgttca gaagcacaaa
    27061 tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt cactgcacgt ttaatttttg
    27121 ctgttttttc aatataagct ggtacgttac ctggacctac acctaaagct ggttttcctg
    27181 tagagtaagc agacttgacc attccagctc cacctgtcgc taagacgatc gcgatacctg
    27241 gatggttcat tagcatagaa gtcgcttcga tcgaaggatg ttcgatccat tggatacaat
    27301 tttcaggagc tccggcagcg atagcagcat cacgtaccac tcgtgctgct tctgcagaag
    27361 atttttgggc acttggatgg aaagcaaaaa cgattggatt tcttgttttc aacgcgatca
    27421 ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg agttactccg caaaccacac
    27481 cgactggttc agcaatttca atcaaccctt tttggacatc ttcattgatc acaccaactg
    27541 ttttatcatg tttgatatta tgccaaatat attcggacgc atacatattt ttgattgctt
    27601 tatcttcgta aacaccgcgg cctgtttcct ctacagccat cttcgctaat ggcatatgtt
    27661 gatccaaagc tgccattgcc atttcatgca cgatatggtc tactttttct tgatcaaagc
    27721 tttccatttc tttcaatgct gcatttgctt tttctgccaa atcatcaatc attgcttgta
    27781 cgtcaattgt cttcgttttt tctttttcta ttactttagc catcattatc ctcctaatgg
    27841 gtcatggtta tttgttaaat aattcacatg tatatcatac aatatttatt tttctatgta
    27901 aacacttttt tgtgattttt ttcacagtta tttttaaata taaaattgct ttatttacag
    27961 gtgtttgtat agaaaaatat ataaagttat ttattattat ttatatgaca cacccgatta
    28021 ttcaatattg agcaatatat cccatgctct tatctctttc ttttttgaat aaaaaatgag
    28081 gtaggacatc tttctgccct acctcgtctt taaataagct ttgatactta tttttcttcc
    28141 ttctcttctg tctcttccag cgtttctgtt gtttcagttt ttacttcaac tgtctcagct
    28201 ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg ttacgacagt ccctggtttg
    28261 accgttttga ttgctgcacg atcgtactca aggaagatcc cttcgcagtc taaaacaact
    28321 gtccgtttgt cattgtcgat ttctgaaaca actccatgta atccgccgat cgttacgact
    28381 gaatcacctg gtttcatgct atctaacaag ttttgacgtt cttgctgttg tttcttttgc
    28441 gaacgtgaca taaaaaacca cattgctaat agcgcgataa acatcacaag cattggtaat
    28501 gttcccattt ttttcacctc actaattctg tatctacaaa aaacacttta gcagaaaaat
    28561 gctgataaca ctagtttttt acttaatttt taaaagattt tcctttagaa gctttttgcg
    28621 ttttctttat tgaacccgta ttcttcaaaa aaggcttctc taaattcaag caaattatca
    28681 tccatgattg cttgacgtac ttgtttcatt agattcaaca agaagtataa attgtggtaa
    28741 gaagtcaaac gaatcccaaa tgtttcatca catttgatca aatggcggat ataggcacgt
    28801 gtatagtttt tacatgtata acaatcacat ttctcatcaa ttggacggaa atcatgggca
    28861 tatttcgcat ttttcactac tagacggcct tgagaagtca tacatgtgcc atttcgagcg
    28921 attcgagtag gcaagacaca gtcaaacatg tctactccac ggataacacc atcgatcaat
    28981 gaatcagcag caccgacacc catcaagtaa cgtggtttgt tttcggggat caacggtgta
    29041 gtgaattcta atacacgatt catttctgat ttaggttccc cgacagataa cccgccgatt
    29101 gagtaaccag ggaaatccat cccgactaga tctttcgcgc tttgacgacg aagatcttca
    29161 aaccctgctc cttggatgat tccaaataat ccttgacggt ctggattagc atgagctttc
    29221 aatcctcgtt ctgcccatcg agacgtacgt tctactgatt ttttcacgta gtcgtaactt
    29281 tcatcgaatg gtggacattc gtcaaaactc atcatgatat cagagcccaa tttattttgg
    29341 atgttgattg ctttttctgg tgataaaaac atacgcgaac cgttcaagtg atttttgaaa
    29401 tgcacgcctt cttcttcgat attccgcata tcacttaatg aaaaaacttg aaagccgcct
    29461 gaatctgtta aaatcggttg atcccagttc ataaatttat gcagtccgcc agcttcttct
    29521 actaagtctt caccagggcg tagccataaa tgatatgtat tacttaaaat aacgccagca
    29581 cccatttctt tcaactcttc tggggacatt gtttttactg tagctaatgt tcctactggc
    29641 atgaacattg gtgtggggaa agttccgtgt ggcgtgatca attcccctaa acgcgcgcca
    29701 gtatgttttt ctttcttgat taaacgatag cgaatggcag gttgcgtcat tcatttccct
    29761 actttctata taaaatatct tgacaactcc caccatcata atcgtttcaa gtgctttttg
    29821 caagttccct cggcttcctt ctactttatt taatattctt ttttttatcc tttttttatg
    29881 aataacaaaa aaaagaaaaa cattatgaca gaaatatttt ttgatacatg gtatattggt
    29941 gttattaaag aaaaggaggg aaaaatcatg caatcttctg cagaattaaa acgccaagca
    30001 aaagagagtc taaaaggacg ttggggacaa gctgttttac taaatctaat ccctacattg
    30061 attggtattg ctactattct tcttattatg attcctatta tcttgtttgc caccaccata
    30121 tatgatggat cagcgagcgt ctcagaagcg acaacttcag gaggcggtag ttcaggaggc
    30181 ggtggcggaa ttatttccag catcattagc gctttgttca tgagcggtat ttcttggact
    30241 tatcttgata ttttacgtgg acaaaaagat cagatcgaac cattcaaaga tgcatttcgt
    30301 ggattcaaag gtttgtttat cggcggagtg atcagcttag ctgtgctgat ctcgatcttt
    30361 acaacattat ggggtttgct attagttatt cctggaatca ttaaaagcta ttcttattct
    30421 caaagctatt ttatttacta cgatatcgtc caagaaacag gacaaacacc taaagtactg
    30481 gatacgatca ctgcaagtcg aaaattgatg gatggttata aaggacaact tttctggtta
    30541 gatctttcat ttatcggctg gcatatctta gcattagcaa ctgctggtat tggttatcta
    30601 tggctcagcc catacatttc tgcaacaaaa gcagcatttt atgaacaatt gccaaaagat
    30661 atttaaaaga gagtgtgact tgtgaccgag ggggtggcag gagaacaccg tttatcgaga
    30721 ggcagtaaaa gaaacattca gcccattagc aaaaaggagg tgtgacaaca ttcatgtcac
    30781 acctcctttt tgtatagttc cagtaaaaag aaaatcaact tagaaacagt taatcattcc
    30841 tcttcttctg cttccggtaa tcgatggaac ttgatcagtt cattcaccgc gattggtacg
    30901 atagataaaa tccctaccaa gaaccagtga ttcaaactga tgtgtactag gccgaatagt
    30961 tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag ctaatgccag taacgccatt
    31021 tctaccaaag ctttattggt cgctaaattg actttgaaga tcgattggct gctgcgtaca
    31081 ttgaacacat gcagaatcga tgaataagct aagatcaaga atgcaacagt ttgaccgaca
    31141 tgctgacttg cgtctaccca tgggctgata gtatcgacat aactacctaa ataataacca
    31201 aacaaggtta cgatggtgaa gacccctgca tttatggcaa ttttttgcaa taaacctcga
    31261 gcaaagatcc cttcattttt cggaatgggt ggctgatcca tgatgccgtg ttccgctggc
    31321 tctcttccta aagcaaatcc tggaagtcca tcagcaacta cattgacaaa cagtagttgc
    31381 acagctgtaa atggtgctcc ccatcctaac agcatcgcaa tcaaaacaat aaagatctcc
    31441 gaaatattac agcttagcaa aaagttgata gctttacgaa tattctgata aaccgaacgt
    31501 ccttgactga cagcgtctac gatcgtcgca aagttgtcat ccgtcaaaat catatctgcg
    31561 gcactttgcg ccacatctgt tcctgtgatc cccatggcac acccaacgtt acttgctttc
    31621 aacgctggtg catcattcac accatcccca gtcatcgcta cgactgcacc cgtacgttgc
    31681 caagatttga cgatccggat cttgtcttct ggcgttaccc gagcatagac agagagagct
    31741 ttgacacgcg catctaattc ttcgtctgat aattgatgca gttctgatcc tgaaagtgct
    31801 tctgacggat cattcaagat acctaattct ttcgctatgg cactggcagt caccacatga
    31861 tctccagtga tcatgactgt tttgatgcca gcctttttcg ctcgcttgat tgctccttta
    31921 ctttctggac gtggtggatc gatcattccg atcagaccaa tcaaacgtaa gtctttttcc
    31981 agtgcttcag aagaaatatc ttgcggttct tcgtcataag tagcataacc aacagcaatc
    32041 actctcagtg cccgtttacc aaagcgatca ttaacgatcg ccgcttgttc tacatcgcct
    32101 gaactaaagc gcggagcaag aacatcgaat gctcctttgg tgattgagat gtatttttcg
    32161 cccatttggt gaactgttgt catcattttt cgttcagaat caaaaggaag ctcgtttact
    32221 cgtggatatt tttcttctaa ttctgccttt gtatgataat tctcttcaac agcccttacg
    32281 atggctactt ctgttggatt acctttgata acaaggtcgt catcttcttg ctcgaccgtc
    32341 acatctgtac atagtgcagc cattttcaat atttccatcg cgctgttcgt cattgaatct
    32401 tcgatattcg ttacttcatc ttctttgctc catacccggc ggacgcgcat ttcattttgt
    32461 gtcagcgttc ctgttttgtc tgagcagata acattagcgg ttcctagcgt ttcaactgct
    32521 ggcaactgtc ggatgatcgc atgtttcctc gccatcttct gcacaccgta tgcaagtgtc
    32581 aacgtaacaa taacagtcaa ggtttccggt acagctgcaa cagccaagga aatcgaagtc
    32641 atgaacatct caaggagtgg ttctccttgc agttcaccaa tcacaaatac taaagccgca
    32701 gccgctaaag cgatcatact gattcgtttg cctagctgat tcaatcggac ctgtaaaggt
    32761 gttttttgca tagtattttc gttcaaaaga tccgcgatct tacccatttc tgtagccatg
    32821 ccaatggcag tgacgatcgc tttcccacga cctgcagcaa ccgtacatcc tttaaatacc
    32881 atattaagtt ggtcacctaa tgactcatct tcttcagcaa taaatgctgc atctttttca
    32941 actgcttcac tttctccagt caacgccgat tcttctacac gtaattgtga tgcttcgatc
    33001 aaacgggcat cggcagaaat agcatcaccg gactctaaaa caagaatatc acccactacc
    33061 agttgctctg actcaacagt ggtcactttg ccgtctcgta tgacagttgc cgtttgttta
    33121 ttcatatctt gcaaggattc cagtgctttt tctgcgtttc cttcttggac aatcgccaat
    33181 accgagttga tcacaacgat agcgatgatc agcagtcctt caaataaatc cccatgttct
    33241 gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa gaaggatgat cgttgtaaaa
    33301 tcggacaagc ttcgaaagaa ttttttgatc atcgattctt tcggcgcctc atcgaattta
    33361 ttcaatccct gcttttttcg ccgttcatcc acttcttgtg aagacaaccc agtatctgga
    33421 tctgcttctg ttaccttctt tacttcttct attgtctgct tgtattcttc catcctattc
    33481 ctcctttaaa atcttcgtac aaaaaaaggt acttatttta ctataacatc ctttttttat
    33541 ttacttcaaa taaagggctt atcgattatt tgttaagaaa gcgtaaaatt taaagtgctt
    33601 atatttgcaa acgctaccaa aggatgctat tatatagatg taaggaagga ggataccatc
    33661 aaatagacag aagaatttct cgatagaaca gtgtaagcaa acaaagttgt tagaagtaca
    33721 ggtttcaccc agacatgcgt gtagagtaca aacctataaa tagtgaatgc gtatggatgt
    33781 ttggatcacg aagtatccga aacaagaagt aatgcgatac gtaacaattg aataacgtaa
    33841 acgtctgaaa attgaatagg aacaagagat gtttcaaatg ttgaagatgt aacgaaaaca
    33901 aaatttttgt ttaacatgaa gaaattcaac accgtgcttt gcatgtatga tggaaagtta
    33961 caatcatttc tgtttatttg aatccttccg aggtacaaga aagccgagct tatcgagagc
    34021 tcggcttttt ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc
    34081 ttttcataac cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct
    34141 tcaatcgcat gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac
    34201 atgactaaag tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat
    34261 tcatagccag gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta
    34321 aatttcgttc caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc
    34381 ccgccatttt tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat
    34441 tcactatgca tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac
    34501 cctacatgta gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat
    34561 tctttcgtaa aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag
    34621 acagtttgat agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt
    34681 tctcccaatg attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt
    34741 ccgccgtgtt ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt
    34801 cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt
    34861 gtattcgtta gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa
    34921 cgagcaggca gaactcgtgt attattcata actagcgcat caccaggctg taactcttca
    34981 atgatttcat ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa
    35041 cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa
    35101 tcaaaatctt ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct
    35161 atcatgtttt tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc
    35221 taaataaaaa aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat
    35281 agttcttcat tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa
    35341 aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg
    35401 agttcatttg ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca
    35461 accgaatgac tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa
    35521 ttgatccagt ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc
    35581 ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag
    35641 aagaacggaa taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt
    35701 tagatcgcta cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg
    35761 cccttgcagc gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa
    35821 tcactcgtga ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga
    35881 tcgtatttgt aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca
    35941 tgagtgaagg ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa
    36001 acgatttcgt agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga
    36061 caagagtcgg cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac
    36121 aagaagcaat caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt
    36181 cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat
    36241 ccagtgtcgg cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc
    36301 gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac
    36361 agaacaaagt cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga
    36421 tttggggaga agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta
    36481 aggaggtctg acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag
    36541 gttttgaaca tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac
    36601 cgcttggtgt cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca
    36661 gtgccttgca gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg
    36721 taggtaaatt acctgcgatt atcgcactct ttatttattc attattgccg attttacgta
    36781 atacgtacat tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg
    36841 ggatgactga gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca
    36901 tggctggtat tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata
    36961 tcggcgctgg tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc
    37021 tcattttggc aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag
    37081 gtctcttgga acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg
    37141 tatactaaaa atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc
    37201 tggtcttgcc agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga
    37261 agcacagatc ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac
    37321 cgcaatcatc aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga
    37381 tgcttcaatt tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc
    37441 tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata
    37501 cggtcaaacg tggttcccgt cctatagttt tgaaaatacg tatgtctttc ttgttcgaaa
    37561 agacaccgca caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga
    37621 attagtagct ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt
    37681 ccaagaaact tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta
    37741 tgacgcagtc gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat
    37801 cgccagttat gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc
    37861 tgctccagtt gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa
    37921 taagttaggt cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa
    37981 taatctagtg gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga
    38041 agggaagtga agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag
    38101 gaaaatggct cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt
    38161 gtcctctttg cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa
    38221 ttagcaaact gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg
    38281 atctccattt taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt
    38341 ttgtattccc tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa
    38401 aacatccttg atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta
    38461 gagttaccac tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc
    38521 atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc
    38581 cgaggaacca atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca
    38641 ttaatggcga tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc
    38701 agccgtacat cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt
    38761 cgcaaaggct tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc
    38821 cttatgggta aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat
    38881 actgccaacg ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca
    38941 ttttgccaat ccattttccc atcactcgtc atggaattca taccacatga tctctaaata
    39001 ctcgtctgct tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat
    39061 ttaagccttt tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg
    39121 tgactgccgt atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat
    39181 ccttggctaa aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct
    39241 aaaaccggta cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg
    39301 atccttcacc agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa
    39361 gacattggcg taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat
    39421 ggccgaaagc gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt
    39481 cgctttggat acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc
    39541 ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt
    39601 gtccattgct tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt
    39661 cttcgcctca ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc
    39721 tccaatttta ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca
    39781 acattccatt cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta
    39841 aagaaatcat gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt
    39901 tggggagact ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt
    39961 gtccaggcag aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga
    40021 tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa
    40081 gactgcgtgg gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc
    40141 tttaaattcg tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact
    40201 gtatagttac tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt
    40261 cgtctttttc gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt
    40321 cttgaatcgc tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac
    40381 taataatctc gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt
    40441 tacgagcagt gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata
    40501 taattatagc gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc
    40561 aaacttttcc tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca
    40621 cgctcacttc ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac
    40681 gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac
    40741 acgaaggttc ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca
    40801 cgaaagattt gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca
    40861 aaaatgattg cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt
    40921 agaccgttgg gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat
    40981 ggtcacttga cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg
    41041 taaaggaaga agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt
    41101 atgatcaaga catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc
    41161 atacgctgtt cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa
    41221 acagaccctc tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt
    41281 tatttcttca aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc
    41341 aatcttaacc gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca
    41401 aattgaggaa tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata
    41461 tctacataac ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa
    41521 cggacatctg ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct
    41581 tcgtctagcg aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac
    41641 gcaccgatat ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa
    41701 tcatgtagtt ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca
    41761 tggatcttat aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc
    41821 cctaaccgat agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata
    41881 ttggcaatcg atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct
    41941 gtttgagata aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct
    42001 gttgagccat ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca
    42061 ttggaatagc ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt
    42121 gccagattgt atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa
    42181 atcagcgctg tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta
    42241 ccaatttgag acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata
    42301 aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg
    42361 gcattttttg ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca
    42421 gttaaagagg aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc
    42481 acagccgctc ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat
    42541 aaaccaacaa cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg
    42601 aacgatgcag attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc
    42661 acagaaatag cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca
    42721 cctgctgaga cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa
    42781 atcaagccag cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga
    42841 ggataagcat ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca
    42901 aaagcggcaa tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca
    42961 acgatttgtt ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa
    43021 gcagcaaatc gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact
    43081 aacaaccttt ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt
    43141 ttatagtcga aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc
    43201 aataaacttt aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg
    43261 ctttaataca gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa
    43321 aaggatagaa cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga
    43381 agagagatgt aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg
    43441 tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa
    43501 attaaaaaca taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct
    43561 tttttacaac ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa
    43621 aacggcaaaa tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac
    43681 tgatttcaca acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat
    43741 ttcgtcattt tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca
    43801 aacaaaccga tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca
    43861 gattcccgta acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag
    43921 aatttccctg agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag
    43981 atgataatag cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc
    44041 atcatgaacg ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca
    44101 tctactccta gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt
    44161 gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct
    44221 ttctttccta aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac
    44281 agacatagtg cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc
    44341 gcattggttt gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt
    44401 ttcaataatt tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct
    44461 gctgcttttt ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca
    44521 gcgttcacag ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg
    44581 atttcatttt ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt
    44641 tgctgaatga tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg
    44701 atggtatcca tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca
    44761 ggcatttctt tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt
    44821 gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc
    44881 ccaatcgtat cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta
    44941 ttgataccag caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat
    45001 tcatcaatag ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt
    45061 tgcagattgc ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc
    45121 gtatttccag cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt
    45181 tgttgttgag atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca
    45241 tcatcagtca gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc
    45301 gttgttgcca cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt
    45361 atcgcatctt tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga
    45421 ttttctaaat ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct
    45481 tgttttgctt catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg
    45541 atctctgcta gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg
    45601 atgatttttg cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca
    45661 cccatctcat ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc
    45721 ttgatttcag gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg
    45781 tccaaatttt catctgttga taaaatcata tctttaactt tattgatact tactaggttc
    45841 gtatcgatgt cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg
    45901 ataaattcat ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt
    45961 gcaatcgcat tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca
    46021 ctagtaaaac tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac
    46081 tttccggaac ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat
    46141 tgtttatttt tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga
    46201 aactctgctg gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat
    46261 ggatcccata atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg
    46321 atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat
    46381 cgaagcgtat ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta
    46441 ctttaccatg tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg
    46501 acatatcacg gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta
    46561 cagcctttac tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct
    46621 attatacgta tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt
    46681 cagctttttt cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc
    46741 cagcaggagg atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag
    46801 acagattcat tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag
    46861 caaaggcttc tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct
    46921 ttttttgccg caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt
    46981 tcatgatctg gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt
    47041 ctgcctgatc agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag
    47101 ctcctaattt ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc
    47161 ccttttcatg gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca
    47221 ttacagtatc tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc
    47281 ctggcgtgac cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt
    47341 ttgctgatgg caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc
    47401 gatcacctac atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat
    47461 cattacctgg tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca
    47521 aggcaacatc ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac
    47581 cgacttctct gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt
    47641 taattgcaaa tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct
    47701 ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt
    47761 cttttttcgc gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct
    47821 aataaaaaat aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa
    47881 aaaacagcaa gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa
    47941 agattacgaa ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg
    48001 tgatttaatc gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa
    48061 attccgtgaa ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc
    48121 caagtctggc tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa
    48181 agatacgttc gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta
    48241 cgcacagctg gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta
    48301 gtaacaggag tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc
    48361 ataatgggtc gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg
    48421 atctgctcca acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca
    48481 atgtgagtcg gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc
    48541 gggctaaaat ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc
    48601 tccgatccca cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga
    48661 tcctccttat tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata
    48721 gtggatcgta cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt
    48781 gccgataagc gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg
    48841 atccatacat acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc
    48901 ctgcatcttt cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc
    48961 ttcaggagtg atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac
    49021 ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc
    49081 gtgcccagag ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt
    49141 ttcatcatgc cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg
    49201 aagttttttc agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc
    49261 ctgatctgct gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt
    49321 gatgaatgca gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc
    49381 agtgtctgct tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc
    49441 agctgctgct tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc
    49501 tacagtcagt gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc
    49561 ttctctcaac tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc
    49621 aactgctgcc tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc
    49681 aaacaattct tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc
    49741 ttttttatgt atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc
    49801 tttctagtct tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac
    49861 ccatacattg ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa
    49921 aatctcgaca tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc
    49981 ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg
    50041 gaatagtttc atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat
    50101 cagccgagtg atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt
    50161 ttctcttttc aataaattta gaacactctc attctaccaa attataacca ttttgccaca
    50221 aataagtcca caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta
    50281 tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc
    50341 gccatctttt tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata
    50401 attaccgccg tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta
    50461 tccttttttc tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc
    50521 tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc
    50581 taataattta tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc
    50641 gatttgttca actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg
    50701 taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt
    50761 tggtgtaacg aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg
    50821 gtgaacgtag ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga
    50881 aatatctaat ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt
    50941 aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc
    51001 acggtaaccg cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc
    51061 gaagacgatt gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt
    51121 ttcgtattct tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc
    51181 tttgatcttc acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg
    51241 cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt
    51301 ttcgatatct tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt
    51361 ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt
    51421 acccacaacg atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc
    51481 gccgtaaacg gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg
    51541 tgtttggata gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc
    51601 tggatcgatt ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg
    51661 tgcttgtcca ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg
    51721 cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag
    51781 ttctttaaat ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg
    51841 ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg
    51901 tttttatcca ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca
    51961 ttagaaaagc caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca
    52021 agttgtttct atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct
    52081 aacatagttt ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc
    52141 taccacttca aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc
    52201 aagattttgt ttgatagcat caatcaattt attcttttct ttttcaaaat agaacacagg
    52261 tagttggggg tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag
    52321 gaaaatcatg gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa
    52381 agactctttc cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg
    52441 cagattttta aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc
    52501 gctcaaaata gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga
    52561 aacatgggcg agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt
    52621 atacgcaatc aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc
    52681 tccttctact acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc
    52741 gccatttcca ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag
    52801 caacggttca tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc
    52861 cattttggct ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat
    52921 cattgtgatt gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc
    52981 gtgatccatg cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt
    53041 gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc
    53101 agacaagaca gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc
    53161 aacgacttct gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa
    53221 aggatctgct acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca
    53281 aaatgaagca gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt
    53341 accagcaaga ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt
    53401 ggtaatcttt gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt
    53461 tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg
    53521 ggttcgcatg actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga
    53581 acgcggttac gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa
    53641 aaagattgac gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca
    53701 ccgtatttca agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct
    53761 ggcatagagt ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac
    53821 cggctcaatc cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt
    53881 tgataaactt cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat
    53941 tttgcttcat aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga
    54001 acgtcttcat ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg
    54061 attgctcgag aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt
    54121 tctgcttttg taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa
    54181 agcaaagaac cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta
    54241 agtactggta cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg
    54301 attttatcca tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat
    54361 gtctctaaga agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa
    54421 acgatggctc cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc
    54481 tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct
    54541 gttagtaatg gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg
    54601 taataattat aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc
    54661 tctgcgctgc gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta
    54721 aatcgttcag tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat
    54781 ttataaaaaa acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga
    54841 ttatttgtcc cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc
    54901 aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag
    54961 tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag
    55021 gccccacctt catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg
    55081 ccttcactca aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg
    55141 tcaaaaagac gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt
    55201 caaaatcgcc ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg
    55261 gtgacgcggt agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc
    55321 ttcaactatt tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg
    55381 atctaaaagg ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa
    55441 aagaaagtca aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca
    55501 gccccaagat ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg
    55561 gtaaaaactt gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa
    55621 ttcaaaacaa gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac
    55681 aggtgtttga tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg
    55741 tactaacaaa agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag
    55801 agttcggttc ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata
    55861 ccatcaacta catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac
    55921 tcacacaagc cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc
    55981 ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa
    56041 gttttcttga acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca
    56101 cacatgtcgt gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt
    56161 tgctgaaagc atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc
    56221 tcatggaagt cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag
    56281 atacattgaa acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc
    56341 ctcaacagct ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg
    56401 aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt
    56461 tcggtatgat tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg
    56521 tagaaaacgc agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg
    56581 tcattatttt tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga
    56641 aaaaggttgt gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt
    56701 ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga
    56761 gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac
    56821 atggaacaca tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa
    56881 taccttcgtt acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac
    56941 gcttgacgaa aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg
    57001 ccagcttttc ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct
    57061 tcgtcaaatt cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa
    57121 gctaaaccat aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta
    57181 taatccgcac tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct
    57241 ttggtcgtat aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct
    57301 aattttatga catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt
    57361 tttcgccttc ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt
    57421 gatccatttt gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag
    57481 cgtgatcaat aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt
    57541 catcaataaa aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat
    57601 ccggcgatcc attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc
    57661 tgttactacg tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa
    57721 gaggaccaat aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa
    57781 acgtttcaaa atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa
    57841 atacataaaa atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa
    57901 taaaaaaagg aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg
    57961 caacttccat tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa
    58021 gaaaccataa atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa
    58081 aagaaacaat aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt
    58141 tcttttgatg tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc
    58201 catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa
    58261 ctcaatcgct tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc
    58321 catattgata aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag
    58381 ccatgctgca ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac
    58441 gcctaatatt ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt
    58501 gtaactatct agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc
    58561 ataaaacagc gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa
    58621 taaaagataa ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa
    58681 attacgccca aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata
    58741 actaatctgc tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat
    58801 tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg
    58861 attactcaag cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc
    58921 aggtgataaa gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa
    58981 accagagtcc ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc
    59041 gagagctttt ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag
    59101 ttcttctgta atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg
    59161 attcactcgc aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg
    59221 aaaaactgaa gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa
    59281 ataaattttt ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt
    59341 taataaaaag taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata
    59401 agttttcagc atgtaatcca cttgtttttg accaatgatc ggcacaaaaa cttccggcca
    59461 tacttcttgt atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc
    59521 catacaccca cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag
    59581 catgatcatg ctatacacac ccaccgacca cggtttcaag aaaatcacaa tctgcataaa
    59641 ttccctaaaa gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa
    59701 gggaagcagc aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa
    59761 ttcatcaaat ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata
    59821 gtaacggaca agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa
    59881 aagcccaaat ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa
    59941 aataggaatg atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc
    60001 aaattgctgg ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc
    60061 ataaagaatc aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt
    60121 ttgtaaaact gatccttttt tagacatcta tattccccac atcttttttc atttattaaa
    60181 actgcctcct t
//
LOCUS       DASJPC010000005        57043 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJPC010000005 DASJPC010000000
VERSION     DASJPC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.005"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            91..783
                     /locus_tag="U4A13_000325"
     CDS             91..783
                     /locus_tag="U4A13_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1778657.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            991..1281
                     /locus_tag="U4A13_000326"
     CDS             991..1281
                     /locus_tag="U4A13_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778658.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1995..3365
                     /locus_tag="U4A13_000327"
     CDS             1995..3365
                     /locus_tag="U4A13_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778659.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3605..3688)
                     /locus_tag="U4A13_000328"
     tRNA            complement(3605..3688)
                     /locus_tag="U4A13_000328"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3652..3654),aa:Leu,seq:gag)
     gene            3743..3922
                     /locus_tag="U4A13_000329"
     CDS             3743..3922
                     /locus_tag="U4A13_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778660.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            4071..5288
                     /locus_tag="U4A13_000330"
     CDS             4071..5288
                     /locus_tag="U4A13_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1778661.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5472..6308
                     /locus_tag="U4A13_000331"
     CDS             5472..6308
                     /locus_tag="U4A13_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1778662.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6384..7532
                     /gene="nagA"
                     /locus_tag="U4A13_000332"
     CDS             6384..7532
                     /gene="nagA"
                     /locus_tag="U4A13_000332"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN1778663.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7752..8810
                     /locus_tag="U4A13_000333"
     CDS             7752..8810
                     /locus_tag="U4A13_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN1778664.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            8814..9848
                     /locus_tag="U4A13_000334"
     CDS             8814..9848
                     /locus_tag="U4A13_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN1778665.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(9950..10666)
                     /locus_tag="U4A13_000335"
     CDS             complement(9950..10666)
                     /locus_tag="U4A13_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1778666.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10635..11420)
                     /locus_tag="U4A13_000336"
     CDS             complement(10635..11420)
                     /locus_tag="U4A13_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN1778667.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11411..12160)
                     /locus_tag="U4A13_000337"
     CDS             complement(11411..12160)
                     /locus_tag="U4A13_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1778668.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12458..12982
                     /locus_tag="U4A13_000338"
     CDS             12458..12982
                     /locus_tag="U4A13_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN1778669.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            12998..14497
                     /locus_tag="U4A13_000339"
     CDS             12998..14497
                     /locus_tag="U4A13_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN1778670.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14585..15187)
                     /locus_tag="U4A13_000340"
     CDS             complement(14585..15187)
                     /locus_tag="U4A13_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1778671.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15291..16247
                     /locus_tag="U4A13_000341"
     CDS             15291..16247
                     /locus_tag="U4A13_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN1778672.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16552..17508)
                     /locus_tag="U4A13_000342"
     CDS             complement(16552..17508)
                     /locus_tag="U4A13_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1778673.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17682..18242
                     /locus_tag="U4A13_000343"
     CDS             17682..18242
                     /locus_tag="U4A13_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778674.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18235..19059
                     /locus_tag="U4A13_000344"
     CDS             18235..19059
                     /locus_tag="U4A13_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN1778675.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19069..19686
                     /locus_tag="U4A13_000345"
     CDS             19069..19686
                     /locus_tag="U4A13_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1778676.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19704..20387
                     /locus_tag="U4A13_000346"
     CDS             19704..20387
                     /locus_tag="U4A13_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN1778677.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21070..22305
                     /locus_tag="U4A13_000347"
                     /pseudo
     CDS             21070..22305
                     /locus_tag="U4A13_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22313..23200
                     /locus_tag="U4A13_000348"
     CDS             22313..23200
                     /locus_tag="U4A13_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1778678.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23217..24053
                     /locus_tag="U4A13_000349"
     CDS             23217..24053
                     /locus_tag="U4A13_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1778679.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24056..24679
                     /locus_tag="U4A13_000350"
     CDS             24056..24679
                     /locus_tag="U4A13_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778680.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24676..25968
                     /locus_tag="U4A13_000351"
     CDS             24676..25968
                     /locus_tag="U4A13_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1778681.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26327..26424
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26540..27121
                     /locus_tag="U4A13_000352"
     CDS             26540..27121
                     /locus_tag="U4A13_000352"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN1778682.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27124..28431
                     /locus_tag="U4A13_000353"
     CDS             27124..28431
                     /locus_tag="U4A13_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN1778683.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28724..29212
                     /gene="purE"
                     /locus_tag="U4A13_000354"
     CDS             28724..29212
                     /gene="purE"
                     /locus_tag="U4A13_000354"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN1778684.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29205..30329
                     /gene="purK"
                     /locus_tag="U4A13_000355"
     CDS             29205..30329
                     /gene="purK"
                     /locus_tag="U4A13_000355"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN1778685.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30342..31637
                     /gene="purB"
                     /locus_tag="U4A13_000356"
     CDS             30342..31637
                     /gene="purB"
                     /locus_tag="U4A13_000356"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN1778686.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(31817..32194)
                     /locus_tag="U4A13_000357"
     CDS             complement(31817..32194)
                     /locus_tag="U4A13_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778687.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32450..33178
                     /locus_tag="U4A13_000358"
     CDS             32450..33178
                     /locus_tag="U4A13_000358"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN1778688.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33178..33432
                     /gene="purS"
                     /locus_tag="U4A13_000359"
     CDS             33178..33432
                     /gene="purS"
                     /locus_tag="U4A13_000359"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN1778689.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33434..34105
                     /gene="purQ"
                     /locus_tag="U4A13_000360"
     CDS             33434..34105
                     /gene="purQ"
                     /locus_tag="U4A13_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN1778690.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34106..36328
                     /gene="purL"
                     /locus_tag="U4A13_000361"
     CDS             34106..36328
                     /gene="purL"
                     /locus_tag="U4A13_000361"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN1778691.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36313..37752
                     /locus_tag="U4A13_000362"
     CDS             36313..37752
                     /locus_tag="U4A13_000362"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN1778692.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37775..38827
                     /locus_tag="U4A13_000363"
     CDS             37775..38827
                     /locus_tag="U4A13_000363"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN1778693.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            38824..39402
                     /gene="purN"
                     /locus_tag="U4A13_000364"
     CDS             38824..39402
                     /gene="purN"
                     /locus_tag="U4A13_000364"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN1778694.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39399..40934
                     /gene="purH"
                     /locus_tag="U4A13_000365"
     CDS             39399..40934
                     /gene="purH"
                     /locus_tag="U4A13_000365"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN1778695.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            40950..42224
                     /gene="purD"
                     /locus_tag="U4A13_000366"
     CDS             40950..42224
                     /gene="purD"
                     /locus_tag="U4A13_000366"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN1778696.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42346..43260
                     /locus_tag="U4A13_000367"
     CDS             42346..43260
                     /locus_tag="U4A13_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1778697.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43393..44958)
                     /locus_tag="U4A13_000368"
     CDS             complement(43393..44958)
                     /locus_tag="U4A13_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1778698.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(44951..46402)
                     /locus_tag="U4A13_000369"
     CDS             complement(44951..46402)
                     /locus_tag="U4A13_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1778699.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46416..47432)
                     /locus_tag="U4A13_000370"
     CDS             complement(46416..47432)
                     /locus_tag="U4A13_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1778700.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47652..48740
                     /locus_tag="U4A13_000371"
     CDS             47652..48740
                     /locus_tag="U4A13_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN1778701.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48794..50320
                     /locus_tag="U4A13_000372"
     CDS             48794..50320
                     /locus_tag="U4A13_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN1778702.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50322..51527
                     /locus_tag="U4A13_000373"
     CDS             50322..51527
                     /locus_tag="U4A13_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1778703.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51784..52761
                     /gene="guaC"
                     /locus_tag="U4A13_000374"
     CDS             51784..52761
                     /gene="guaC"
                     /locus_tag="U4A13_000374"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN1778704.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53025..54449
                     /locus_tag="U4A13_000375"
     CDS             53025..54449
                     /locus_tag="U4A13_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1778705.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            54910..56019
                     /gene="pdhA"
                     /locus_tag="U4A13_000376"
     CDS             54910..56019
                     /gene="pdhA"
                     /locus_tag="U4A13_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN1778706.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56022..56999
                     /locus_tag="U4A13_000377"
     CDS             56022..56999
                     /locus_tag="U4A13_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN1778707.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        18378 a         9689 c        12385 g        16591 t
ORIGIN      
        1 aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta cttttagcat
       61 gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac tggtgggatt
      121 gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa tgaaattatt
      181 ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca gcttgatcat
      241 acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc acaaaaagaa
      301 cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt aaatgaatta
      361 gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca attagtcagc
      421 aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga tgagatcaac
      481 caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact acaagaagca
      541 caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca aatcgcaaca
      601 ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa gaaattagac
      661 gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga aaaagcaaaa
      721 gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca tttgcgtaaa
      781 taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc tatactgctg
      841 cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg attttaagaa
      901 aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa agggaatgta
      961 tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac agtagataag
     1021 atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa tggggtcaat
     1081 tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg gatgaagtta
     1141 gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta tcatgtgtta
     1201 gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa aaaggtccga
     1261 caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct agccgcttgt
     1321 tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg ctcacaagaa
     1381 cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact aaactttcca
     1441 aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc ctattagtat
     1501 tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag tctcaggtag
     1561 tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg aagaggtgat
     1621 tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa cgtcctggtt
     1681 cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct tgttcgtcaa
     1741 ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc tgcattactg
     1801 caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat ttttttgttg
     1861 actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat ttaaaagata
     1921 acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta ggaaatataa
     1981 gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa gaactgatct
     2041 tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg ccgactatga
     2101 gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg ggtgcttcga
     2161 tagaacatta taagacatgg agttcaagaa ttattataga actattctta atgttctttt
     2221 ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga attgtagtat
     2281 tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg gtttgctcta
     2341 tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca accaccttga
     2401 actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt caactcttaa
     2461 gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg atatttttaa
     2521 ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt agtttatatt
     2581 tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata agcttagcag
     2641 aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat gaaattaata
     2701 aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta ggtatttcat
     2761 catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta tttttcttaa
     2821 ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg actgcattac
     2881 cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc tttacttacg
     2941 tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat ctctttacag
     3001 agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact ttagtagtct
     3061 tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat aagaaaactt
     3121 ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt atgggatttt
     3181 ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt gttatcgcca
     3241 tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa aaaaatgaat
     3301 ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg gtgataaaca
     3361 gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact cgtttttcga
     3421 ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct tcatatactc
     3481 acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg atatgtatat
     3541 aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac ttcacgagtt
     3601 gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac cctcaatcat
     3661 gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat agcgaggttt
     3721 tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat atgacggaga
     3781 attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg gacgtttttt
     3841 gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag cgaatctatg
     3901 gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta atacttacgt
     3961 ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa tagtaaataa
     4021 caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg atgagagaat
     4081 ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc gttctagaag
     4141 aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg ggaaatcccg
     4201 caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg aatgcccgtg
     4261 actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca attgagcaat
     4321 attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc ggcaacggag
     4381 tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat gaagtactgg
     4441 ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga ggcacacctg
     4501 ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat atcaaatcaa
     4561 aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct actggggcac
     4621 tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat aacctgatta
     4681 tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat attccgattg
     4741 ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa tctcatcgtg
     4801 tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac gtcaaaggct
     4861 atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta ttgtctcagc
     4921 aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc ctgccaggcg
     4981 gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt cctggtcttt
     5041 cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca aagcgattca
     5101 atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat attctacttg
     5161 tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt tatcttccta
     5221 aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca acctatcgcc
     5281 aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac acactgaggc
     5341 tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg caagtctttt
     5401 cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg catattttgg
     5461 ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa gagattcgaa
     5521 aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa agtgtattaa
     5581 gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag atttgtcaac
     5641 ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact cggacaaacg
     5701 ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg aagttagaac
     5761 aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac tttcaacgct
     5821 attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct cagtcccttt
     5881 attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat gtttctgaca
     5941 cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga aaaacggctg
     6001 aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct gatgaacgat
     6061 caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc catcaaaatg
     6121 aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa gagaagaata
     6181 gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag cgtaaagaag
     6241 aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa atttcaaaaa
     6301 atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac tttagaggta
     6361 gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa ttctttttag
     6421 cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt ggtgaataca
     6481 ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa tggattgctc
     6541 ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg gataatgatg
     6601 cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact tcatttttac
     6661 caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca atcggcaaaa
     6721 tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt cctttcttca
     6781 cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat ttggatactt
     6841 tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta gcaccggaac
     6901 gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt gtttctctag
     6961 gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc gcaagtgtat
     7021 ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt atggtgggtg
     7081 cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat catgtacatc
     7141 caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca ttaattacag
     7201 actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa tttccagtag
     7261 tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt atattgaaat
     7321 tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa caagcagtca
     7381 tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt ggaaaaatcg
     7441 ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg gaagcaactt
     7501 atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct taaaactaaa
     7561 cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt tacataagtt
     7621 tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat aaaattaaaa
     7681 atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg aacataatta
     7741 ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat atgtcgattg
     7801 tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt agaaaaaagg
     7861 ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca gtggctttgc
     7921 cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg atgcaaggaa
     7981 tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt tcaaaacctt
     8041 ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg atctttggtt
     8101 ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc ggcgtgatgt
     8161 attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga tacataaaaa
     8221 tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat ccaatcatta
     8281 tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca caagtgtcag
     8341 ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac cagactgcac
     8401 cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg gcgtggctag
     8461 caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa aaaacttcat
     8521 ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg attctagcag
     8581 ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca atcgttatca
     8641 tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt gatagggagg
     8701 cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta ctgacacctg
     8761 tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa gaaatggagg
     8821 aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat ttttttgaaa
     8881 aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca catgacaata
     8941 tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata gcagatgaga
     9001 ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg actgtgggag
     9061 tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc cgtgtaccat
     9121 cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg cttttgagaa
     9181 atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat gagcaaatgt
     9241 ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg atagggttga
     9301 cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat attcatcaga
     9361 cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta gccaaaagcg
     9421 atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg atcaatgaag
     9481 gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt ggagagcttc
     9541 aagataatga agctatttta agagctttga aaagcaatca tttagctggt tttgcaaccg
     9601 atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg gagacgattc
     9661 cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta accccgcatg
     9721 taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc gagaactttc
     9781 gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa gcccgtcaat
     9841 taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc aagtaaacag
     9901 ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact caacagtaat
     9961 ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc tggccgattt
    10021 ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc aaatatttct
    10081 tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa aaaatagtat
    10141 cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga cctttaggca
    10201 aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact tgatcgatca
    10261 atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga tacttttcga
    10321 tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag gcaccgtagc
    10381 ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc actgggatat
    10441 catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca ggcatgtata
    10501 catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct tcttctacat
    10561 ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta cgattcaatt
    10621 ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg atcttccttt
    10681 tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa ttctaaaaat
    10741 ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg attgattccc
    10801 ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat cccgacaccg
    10861 ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa atgaatccgg
    10921 acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc aagtaagttc
    10981 cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg gataaaagaa
    11041 tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt gcctaataaa
    11101 aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc ttttttataa
    11161 ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa atcaatcaaa
    11221 ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat ggagtttata
    11281 tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg atttcgtaaa
    11341 gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct agaaatagtt
    11401 tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc aatcgtctct
    11461 ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa tccaatagct
    11521 gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata gttttatcag
    11581 aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt tcacttaact
    11641 gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt tgggcaagaa
    11701 aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca atcatttttt
    11761 gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa actgcaccaa
    11821 taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct aacattcctt
    11881 ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca tgacttgaga
    11941 aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca gggtccatga
    12001 caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct tgaatcgcat
    12061 gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca acgattttag
    12121 caatgctaga gattttatct gacacactag atcggttcat cgaaagaata aaattcctga
    12181 tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca aggatgatcg
    12241 tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac ctcgaaagaa
    12301 tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac gataaacaca
    12361 accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat tttcaaacat
    12421 tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa tcgcacttga
    12481 gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat tgattatttt
    12541 tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc cttggttaac
    12601 tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat ctttattttt
    12661 atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt tctatatatt
    12721 gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc tggaaggaca
    12781 agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc agttgaaaag
    12841 tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac agacagctga
    12901 gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg tcatgcctta
    12961 tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag ttgtttttat
    13021 cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg tagcgaagta
    13081 ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag gaagcaaaaa
    13141 tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg gcgcattcgt
    13201 cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat tggacgtctc
    13261 cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta cttcgatcat
    13321 caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt taggcaagac
    13381 aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc gctcgattct
    13441 tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc aaagcgtaca
    13501 agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt tcactatcaa
    13561 agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa tcgcccaagt
    13621 caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta tcaaaaaagc
    13681 tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga tcgcagaatc
    13741 attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg ttgctaaagc
    13801 aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag taatcgaaca
    13861 ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag aaaaagaaat
    13921 cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg ccgatcggta
    13981 cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag ccgaagcaga
    14041 acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt tagctggaca
    14101 agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa aacaaaagat
    14161 cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta tcgatatgct
    14221 accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa tctctgttgt
    14281 cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa attatgccac
    14341 aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg acttgaagga
    14401 aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt atgaagcgcc
    14461 aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc accgttcaat
    14521 caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt attgcaaaag
    14581 aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc tcttgtaaat
    14641 ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat agttcttcct
    14701 tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg aatgtcccta
    14761 taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga gggatgctca
    14821 acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg ttaataccat
    14881 ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct accaaaaaat
    14941 cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca cctaatgtat
    15001 tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga cgcaaagaaa
    15061 aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga aaaaacaaga
    15121 tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga aaacggccaa
    15181 attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt ttgtctgata
    15241 gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa atgcgtatag
    15301 gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca gcgggtagct
    15361 tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta ggaaatacca
    15421 agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta gctacggcaa
    15481 cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt ctttttttat
    15541 tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta gtaaattcgg
    15601 ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc ggcaatgaag
    15661 cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac atgccagatc
    15721 gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga aatattttga
    15781 gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc aaatgcgaac
    15841 atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa gaaaactact
    15901 ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca caggccaatg
    15961 tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat tatggaaaac
    16021 atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat gatggcagac
    16081 aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct ctgccgacta
    16141 gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct tttaaaagtt
    16201 ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg ttgtgaattc
    16261 agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg tgttctcctg
    16321 tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt ttttgaaatt
    16381 gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa cggtgttcaa
    16441 aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc tatttttgtt
    16501 tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa attacatatt
    16561 tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca agaagagaga
    16621 gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt aattcattgc
    16681 ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata agatctccgc
    16741 acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg caacgatacc
    16801 gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat tttctttgtt
    16861 acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca ttgtagaggc
    16921 aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat acgtaccgat
    16981 atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag ggtctggttc
    17041 tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc ctgtatccat
    17101 tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt ctgctaagat
    17161 caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat tcatcgtgat
    17221 ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat ggtcgatatg
    17281 ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta acatttcttg
    17341 atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg ctttttctga
    17401 agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt cggcaaactc
    17461 atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct gaaaacgctc
    17521 cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc acgaagaata
    17581 aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga ggtgcttttt
    17641 gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa gtatatattg
    17701 atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt ttagatgaat
    17761 acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa aatctgccat
    17821 ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg ctaacgcaaa
    17881 agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt gcagagtaca
    17941 agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca aaagtcgagc
    18001 tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa gtaagtattc
    18061 aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca gatgttcaaa
    18121 aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat ctggaagaaa
    18181 cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt tcaagtgaat
    18241 aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta tgttattttt
    18301 agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac aagcaccaaa
    18361 acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa ggacaaagaa
    18421 acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg gaagctagtt
    18481 ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca attggcatca
    18541 ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc atttgccaag
    18601 gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc tgtatcatcc
    18661 caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg tgtatcagaa
    18721 gaggaggcga caaagattac taaagaaacg attacggagc caggatacag cgaacaccac
    18781 acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc ttctactgta
    18841 ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa tgctgcgcag
    18901 tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat cacatatgaa
    18961 ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa acaccaattg
    19021 acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat ggcgaaaaaa
    19081 aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc cggtgtagca
    19141 gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt tactgataat
    19201 cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt tccgcatgcc
    19261 caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca agctatatta
    19321 gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt cgggatcaag
    19381 gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa cgaagaatgg
    19441 ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat ggatgaccat
    19501 acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa agtgattacg
    19561 gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc gacagacccc
    19621 ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca gtatgaccgg
    19681 agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc atgacggaat
    19741 tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg atcaagatat
    19801 ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata cgtaatacaa
    19861 atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt actaaaagca
    19921 ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg acacaaggtg
    19981 ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg gtcgttttga
    20041 acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat attccagtta
    20101 tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct ggtatttcga
    20161 ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt cgtaaaaaat
    20221 atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt gctgagacca
    20281 ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag ctttttagtc
    20341 gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag gtgagaatca
    20401 aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag tactatttgt
    20461 tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta cataagaaga
    20521 gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt tattgaagcg
    20581 ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat ttagtagcag
    20641 ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc catagtcaat
    20701 gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag ttatttttct
    20761 gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc aaggatattg
    20821 ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt gaagcaagaa
    20881 gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa gtattgagtt
    20941 gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg aaaaagatcg
    21001 tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat ggaagtagag
    21061 gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat gttattcttc
    21121 ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc ttggtggggt
    21181 tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga aaaaaatccc
    21241 catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa attaggtaca
    21301 ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt tcctcaatac
    21361 atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga tatttctaat
    21421 gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg actgtctaac
    21481 ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt aaatatccct
    21541 gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca aatcactgaa
    21601 gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg tattattcaa
    21661 ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc taacagcttg
    21721 ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa tctggtagac
    21781 aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct gtcttttcag
    21841 gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt gacagaaata
    21901 ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg cagaaaagca
    21961 gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc aaaaagctca
    22021 gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga agaagccaat
    22081 gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga acagctcgct
    22141 cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat ttccgatatg
    22201 gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca agaccaatac
    22261 aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag gcatgaaaaa
    22321 agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat ttgcttctcc
    22381 attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac tttattattc
    22441 gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata attatataaa
    22501 gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct atgtatttgc
    22561 atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa cgaggaatat
    22621 caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg gtggcagtat
    22681 ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga atattttcct
    22741 ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg tcatgatccc
    22801 tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg ctgcaggtat
    22861 caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga acaaatggca
    22921 gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata acttggttat
    22981 gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc aaggtggtcc
    23041 gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt cttttaattc
    23101 tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag ctgtcacctt
    23161 tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag gaagaaatga
    23221 ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt ctagtcatga
    23281 tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag attatgagtg
    23341 gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa ggatgggcgg
    23401 ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc tctgcagttt
    23461 cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg agaatcgatt
    23521 tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt cctgcacaag
    23581 tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg acgtatgtgc
    23641 cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc atgcaattca
    23701 tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc agcaagtata
    23761 gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg acgatcatca
    23821 tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta aacaagccga
    23881 gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct agtacgactt
    23941 acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg attttcttat
    24001 tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc tgaatatgaa
    24061 aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc ggattttttt
    24121 aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt tcactttagg
    24181 attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt atccttctta
    24241 ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag ggaaaatcaa
    24301 acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg cagttgattg
    24361 gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat tgtgtctgtt
    24421 gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag aagataaagc
    24481 agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa acgtcaaggg
    24541 gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc ctgatttttt
    24601 actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct taaaagaaaa
    24661 ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg gagagcgcgt
    24721 tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt cggtttttgt
    24781 gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa gtatggacag
    24841 aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga cgtacggccg
    24901 gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat tcgtgcaatg
    24961 aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga aaaagctcaa
    25021 gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt taattatcgt
    25081 tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat tggtgatgtg
    25141 ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta tttcagaaga
    25201 tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac ccatcatttt
    25261 gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt cggtggatta
    25321 aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta cagtcgatgc
    25381 tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc caatgaggaa
    25441 ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga ccgcagtgta
    25501 ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata tgaaactggt
    25561 gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa tgtttctttc
    25621 aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat caatgcaggt
    25681 ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta tccgatgttc
    25741 ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg aggagatccg
    25801 cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa tcgggcagca
    25861 actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca ttctattgct
    25921 tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga atagatttac
    25981 ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct gtcttttgaa
    26041 cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc tttttatgtg
    26101 ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac aaaaaggaat
    26161 aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg tcagtttcag
    26221 aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa atatggcaaa
    26281 ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt agttttacat
    26341 acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg taaatgctgg
    26401 actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt ggagggtgtt
    26461 tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg tattaataaa
    26521 tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag acggacgtat
    26581 attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag accctgtatt
    26641 gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa tcacgaaagt
    26701 tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag aattagatgt
    26761 tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat tattgacagc
    26821 ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc gtaaattttt
    26881 atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac aagcggcaaa
    26941 aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg ggatcgtcat
    27001 cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga gagtcgtttc
    27061 tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg aggacgcata
    27121 atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca gcatctgcta
    27181 gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact aggctttaat
    27241 caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt agcgacatta
    27301 ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt aggatgtgcg
    27361 atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg agctatctac
    27421 ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt ttctaaaatt
    27481 aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg tttgactttg
    27541 attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga ttttggtgac
    27601 aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt gcaaatgctg
    27661 gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg aagcatctta
    27721 gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt gttccatgtt
    27781 ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt actaatgatc
    27841 atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact tggtgacatt
    27901 actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga aggacttgcc
    27961 gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca aaatgtcgga
    28021 ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc attcttcttg
    28081 attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc tgagccggta
    28141 cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat ccgtatgctg
    28201 ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc tgttggattt
    28261 gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac attgcaaatg
    28321 ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa tttgatcttt
    28381 aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta atagagcaaa
    28441 agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg gaaaaatagt
    28501 tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg aacaccgttt
    28561 atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat ctttaataaa
    28621 cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca tcaaaaaaag
    28681 tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag caaaaatctc
    28741 tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc aaatattgga
    28801 tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc cagatttgat
    28861 gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg caggagcagg
    28921 gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg ttatcggtgt
    28981 accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg tccaaatgcc
    29041 tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta atgctggact
    29101 acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat tggctgaaag
    29161 acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt aaacctttgt
    29221 tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg atggcgatta
    29281 gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat tgtccagccg
    29341 cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg ctgaaagagc
    29401 ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta gaggcgttaa
    29461 atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg attacgcaag
    29521 accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct ccttatgcaa
    29581 cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat ccttgtgttt
    29641 tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa agtcgggcgg
    29701 atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa gcatggatcc
    29761 cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat ttcacgactt
    29821 ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt gctccagccg
    29881 cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt gcagaagcag
    29941 tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc gggctatatg
    30001 tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa gcttgttcta
    30061 tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga gaaaaagtac
    30121 gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg gaaagctatc
    30181 gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa caagcaaaaa
    30241 aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa acgctggaag
    30301 aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa cgttatacac
    30361 gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg ctcgaagtcg
    30421 agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa gatgtgaaaa
    30481 aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa aaattaacac
    30541 gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa gaaagaaaat
    30601 gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc tatctcatca
    30661 agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc attggcgaaa
    30721 aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc catgctgaac
    30781 caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg aacatcgaac
    30841 gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca gtgggaacgt
    30901 tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg atccgtccac
    30961 aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc tcagcgatgg
    31021 cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg caaaaatccg
    31081 aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct gctatgccgc
    31141 acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg gtgcgcggac
    31201 acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt tctcattctt
    31261 cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg ttaaatcgtt
    31321 ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg aatatgtctg
    31381 caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat aagggaatga
    31441 caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg gacaaccaag
    31501 tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta tctaaagaag
    31561 aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg atattcaaac
    31621 gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag aatagcaaaa
    31681 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc
    31741 ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat
    31801 acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat
    31861 ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga
    31921 tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt
    31981 cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg
    32041 ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt
    32101 ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata
    32161 aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta
    32221 tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga
    32281 tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat
    32341 gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa
    32401 caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa
    32461 agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat
    32521 ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag
    32581 tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag
    32641 aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt
    32701 acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg
    32761 tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct actataagga
    32821 tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa
    32881 accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga
    32941 attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa
    33001 tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg
    33061 tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt
    33121 ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg
    33181 tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca
    33241 gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa
    33301 tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt
    33361 tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg
    33421 gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga
    33481 tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga
    33541 gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta
    33601 tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc
    33661 tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg
    33721 acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc
    33781 attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta
    33841 tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact
    33901 aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa
    33961 cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg
    34021 tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa
    34081 aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat
    34141 caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga
    34201 agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg
    34261 gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg
    34321 acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact
    34381 agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg
    34441 agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat
    34501 tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga aatatttact
    34561 agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg
    34621 cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt
    34681 aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat
    34741 catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga
    34801 agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat
    34861 ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat
    34921 aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa
    34981 agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac
    35041 accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg
    35101 agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg
    35161 taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct
    35221 gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc
    35281 tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca
    35341 agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac
    35401 ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat
    35461 gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct
    35521 gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat
    35581 tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa
    35641 accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac
    35701 gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc
    35761 aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac
    35821 tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga
    35881 ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa
    35941 ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg
    36001 attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc
    36061 agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt
    36121 tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga
    36181 agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga
    36241 aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga
    36301 ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt
    36361 gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat
    36421 cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga
    36481 gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga
    36541 gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt
    36601 cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg
    36661 atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac
    36721 tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat
    36781 aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa
    36841 acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg
    36901 aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt
    36961 tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt
    37021 gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc
    37081 agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg
    37141 aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct
    37201 ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta
    37261 aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt
    37321 gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc
    37381 gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca
    37441 ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac
    37501 gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc
    37561 aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg
    37621 atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat
    37681 tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta
    37741 tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc
    37801 aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt
    37861 caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga
    37921 tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac
    37981 gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc
    38041 gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt
    38101 agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg
    38161 atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta
    38221 ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt
    38281 attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat
    38341 tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt
    38401 atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa
    38461 aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca
    38521 tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc
    38581 acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg
    38641 tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc
    38701 agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt
    38761 ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga
    38821 acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat
    38881 tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa
    38941 gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa
    39001 gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag
    39061 atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat
    39121 tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat
    39181 ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc
    39241 gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag
    39301 gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa
    39361 gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg
    39421 tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa
    39481 tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga
    39541 tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta
    39601 aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa
    39661 cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga
    39721 ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta
    39781 gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg
    39841 actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac
    39901 aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat
    39961 acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa
    40021 aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc
    40081 ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata
    40141 ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg
    40201 tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag
    40261 cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga
    40321 atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca
    40381 ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat
    40441 tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct
    40501 taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag
    40561 tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag
    40621 ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa
    40681 tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag
    40741 agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg
    40801 aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc
    40861 aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc
    40921 attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc
    40981 gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg
    41041 cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag
    41101 atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc
    41161 ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct
    41221 tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa
    41281 tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag
    41341 cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg
    41401 ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt
    41461 tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag
    41521 gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt
    41581 cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg
    41641 cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt
    41701 taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg
    41761 cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg
    41821 atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg
    41881 atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg
    41941 ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt
    42001 ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag
    42061 ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac
    42121 agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag
    42181 atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt
    42241 tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag
    42301 agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa
    42361 ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat
    42421 gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac
    42481 catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa
    42541 acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg
    42601 atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg
    42661 aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag
    42721 gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc
    42781 agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta
    42841 gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc
    42901 aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg
    42961 catgatatga cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt
    43021 atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat
    43081 caaacgacac caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa
    43141 gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt
    43201 aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa
    43261 agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct
    43321 gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac
    43381 ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg
    43441 agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat
    43501 ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc
    43561 gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt
    43621 actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg
    43681 atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt
    43741 ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg
    43801 gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat
    43861 ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa
    43921 taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta
    43981 cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac
    44041 agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt
    44101 aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct
    44161 gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa
    44221 ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga
    44281 atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt
    44341 ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact
    44401 gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga
    44461 ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa
    44521 atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa
    44581 atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt
    44641 aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa
    44701 tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt
    44761 caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat
    44821 aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga ttggataata
    44881 agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat
    44941 gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca
    45001 gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc
    45061 cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg
    45121 aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa
    45181 tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg gtaatacgaa
    45241 taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct
    45301 gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt
    45361 caagcatatg gccgtatctt atttttaaga tgactagata atcattgata tggctaattt
    45421 ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa
    45481 gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg
    45541 catccaaact gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac
    45601 tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg
    45661 ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac
    45721 gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt
    45781 cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa
    45841 gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt
    45901 tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag
    45961 tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga
    46021 tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat
    46081 ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc
    46141 taagttcttc taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca
    46201 agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt
    46261 ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca
    46321 gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt
    46381 ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta
    46441 gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt
    46501 cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca
    46561 ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct
    46621 tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc
    46681 gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat
    46741 aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca
    46801 ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt
    46861 agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg
    46921 gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag
    46981 tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg
    47041 gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc
    47101 cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa
    47161 atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga
    47221 ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt
    47281 gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact
    47341 agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca
    47401 acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag
    47461 tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc
    47521 aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc
    47581 ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg
    47641 ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg
    47701 caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg
    47761 taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg
    47821 tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag
    47881 aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg
    47941 cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca
    48001 aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt
    48061 ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga
    48121 aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg
    48181 cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt
    48241 tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt
    48301 ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg
    48361 atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg
    48421 tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg
    48481 ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta
    48541 tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg
    48601 tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata
    48661 cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag
    48721 aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga
    48781 gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc
    48841 tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt
    48901 gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc
    48961 atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt
    49021 gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta
    49081 tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga
    49141 ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga
    49201 cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa
    49261 agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga
    49321 agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc
    49381 catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt
    49441 acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat
    49501 tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg
    49561 agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat
    49621 cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact
    49681 gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa
    49741 gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc
    49801 cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct
    49861 gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt
    49921 attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa
    49981 aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt
    50041 tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg
    50101 gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg
    50161 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
    50221 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
    50281 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
    50341 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
    50401 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
    50461 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
    50521 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
    50581 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
    50641 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
    50701 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
    50761 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
    50821 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
    50881 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
    50941 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
    51001 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
    51061 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
    51121 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
    51181 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
    51241 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
    51301 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
    51361 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
    51421 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
    51481 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
    51541 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
    51601 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
    51661 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
    51721 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
    51781 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
    51841 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
    51901 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
    51961 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
    52021 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
    52081 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
    52141 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
    52201 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
    52261 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
    52321 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
    52381 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
    52441 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
    52501 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
    52561 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
    52621 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
    52681 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
    52741 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
    52801 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
    52861 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
    52921 agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt
    52981 cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag
    53041 agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa
    53101 acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg
    53161 gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa
    53221 cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc
    53281 caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc
    53341 aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg
    53401 agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct
    53461 tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac
    53521 ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg
    53581 gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag
    53641 ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt
    53701 tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc
    53761 ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag
    53821 tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa
    53881 ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta
    53941 tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa
    54001 aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag
    54061 cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat
    54121 ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc
    54181 gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg
    54241 gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag
    54301 tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa
    54361 tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga
    54421 aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa
    54481 cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag
    54541 aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt
    54601 tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat
    54661 tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg attttattca
    54721 ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt
    54781 agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac
    54841 agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat
    54901 gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc
    54961 aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat
    55021 ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct
    55081 cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca
    55141 ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat
    55201 gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct
    55261 caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa
    55321 gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta
    55381 ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca
    55441 tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta
    55501 ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca
    55561 aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg
    55621 gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat
    55681 ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact atctggtgac
    55741 gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg
    55801 atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa
    55861 gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca
    55921 aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa
    55981 gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga
    56041 tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt
    56101 ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa
    56161 agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga
    56221 gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta
    56281 ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg
    56341 gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag
    56401 aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga
    56461 ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc
    56521 cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg
    56581 aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta
    56641 ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag
    56701 aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga
    56761 tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag
    56821 cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag
    56881 ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg
    56941 tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag
    57001 aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DASJPC010000006        50334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJPC010000006 DASJPC010000000
VERSION     DASJPC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.006"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            129..1469
                     /locus_tag="U4A13_000378"
     CDS             129..1469
                     /locus_tag="U4A13_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1778708.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1687..2130
                     /locus_tag="U4A13_000379"
     CDS             1687..2130
                     /locus_tag="U4A13_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN1778709.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2634..4052)
                     /gene="nhaC"
                     /locus_tag="U4A13_000380"
     CDS             complement(2634..4052)
                     /gene="nhaC"
                     /locus_tag="U4A13_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN1778710.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4455..5678)
                     /locus_tag="U4A13_000381"
     CDS             complement(4455..5678)
                     /locus_tag="U4A13_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN1778711.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5709..6548)
                     /locus_tag="U4A13_000382"
     CDS             complement(5709..6548)
                     /locus_tag="U4A13_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1778712.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6565..7245)
                     /locus_tag="U4A13_000383"
     CDS             complement(6565..7245)
                     /locus_tag="U4A13_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778713.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7247..8320)
                     /locus_tag="U4A13_000384"
     CDS             complement(7247..8320)
                     /locus_tag="U4A13_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1778714.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8376..8614)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8657..8890)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8967..9638)
                     /locus_tag="U4A13_000385"
     CDS             complement(8967..9638)
                     /locus_tag="U4A13_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1778715.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9725..12403)
                     /gene="mgtA"
                     /locus_tag="U4A13_000386"
     CDS             complement(9725..12403)
                     /gene="mgtA"
                     /locus_tag="U4A13_000386"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN1778716.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            13062..13499
                     /gene="ndk"
                     /locus_tag="U4A13_000387"
     CDS             13062..13499
                     /gene="ndk"
                     /locus_tag="U4A13_000387"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN1778717.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13521..14387)
                     /locus_tag="U4A13_000388"
     CDS             complement(13521..14387)
                     /locus_tag="U4A13_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1778718.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14547..16640)
                     /locus_tag="U4A13_000389"
     CDS             complement(14547..16640)
                     /locus_tag="U4A13_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1778719.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17389..18012)
                     /locus_tag="U4A13_000390"
     CDS             complement(17389..18012)
                     /locus_tag="U4A13_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778720.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18171..19568)
                     /locus_tag="U4A13_000391"
     CDS             complement(18171..19568)
                     /locus_tag="U4A13_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1778721.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19600..20571)
                     /locus_tag="U4A13_000392"
     CDS             complement(19600..20571)
                     /locus_tag="U4A13_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1778722.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20945..21370
                     /locus_tag="U4A13_000393"
     CDS             20945..21370
                     /locus_tag="U4A13_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778723.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21858..22559)
                     /locus_tag="U4A13_000394"
     CDS             complement(21858..22559)
                     /locus_tag="U4A13_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1778724.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22575..23015)
                     /locus_tag="U4A13_000395"
     CDS             complement(22575..23015)
                     /locus_tag="U4A13_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1778725.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(23015..23590)
                     /locus_tag="U4A13_000396"
     CDS             complement(23015..23590)
                     /locus_tag="U4A13_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN1778726.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(24438..24917)
                     /locus_tag="U4A13_000397"
                     /pseudo
     CDS             complement(24438..24917)
                     /locus_tag="U4A13_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(25076..25777)
                     /locus_tag="U4A13_000398"
     CDS             complement(25076..25777)
                     /locus_tag="U4A13_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1778727.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25885..27294)
                     /locus_tag="U4A13_000399"
     CDS             complement(25885..27294)
                     /locus_tag="U4A13_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN1778728.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(27342..29207)
                     /locus_tag="U4A13_000400"
     CDS             complement(27342..29207)
                     /locus_tag="U4A13_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1778729.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29328..30179)
                     /locus_tag="U4A13_000401"
     CDS             complement(29328..30179)
                     /locus_tag="U4A13_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1778730.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30489..31202)
                     /locus_tag="U4A13_000402"
     CDS             complement(30489..31202)
                     /locus_tag="U4A13_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN1778731.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31270..31596)
                     /locus_tag="U4A13_000403"
     CDS             complement(31270..31596)
                     /locus_tag="U4A13_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1778732.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31719..31946)
                     /locus_tag="U4A13_000404"
     CDS             complement(31719..31946)
                     /locus_tag="U4A13_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN1778733.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32268..33521)
                     /locus_tag="U4A13_000405"
     CDS             complement(32268..33521)
                     /locus_tag="U4A13_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1778734.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33582..34322
                     /locus_tag="U4A13_000406"
     CDS             33582..34322
                     /locus_tag="U4A13_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1778735.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34408..35166)
                     /locus_tag="U4A13_000407"
     CDS             complement(34408..35166)
                     /locus_tag="U4A13_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1778736.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35163..36191)
                     /locus_tag="U4A13_000408"
                     /pseudo
     CDS             complement(35163..36191)
                     /locus_tag="U4A13_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(36211..39378)
                     /locus_tag="U4A13_000409"
     CDS             complement(36211..39378)
                     /locus_tag="U4A13_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778737.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39602..40486)
                     /locus_tag="U4A13_000410"
     CDS             complement(39602..40486)
                     /locus_tag="U4A13_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1778738.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40581..41018)
                     /locus_tag="U4A13_000411"
     CDS             complement(40581..41018)
                     /locus_tag="U4A13_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778739.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(41235..42146)
                     /locus_tag="U4A13_000412"
     CDS             complement(41235..42146)
                     /locus_tag="U4A13_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1778740.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(42603..43031)
                     /locus_tag="U4A13_000413"
     CDS             complement(42603..43031)
                     /locus_tag="U4A13_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778741.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(43059..43697)
                     /locus_tag="U4A13_000414"
     CDS             complement(43059..43697)
                     /locus_tag="U4A13_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778742.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(43701..45683)
                     /locus_tag="U4A13_000415"
     CDS             complement(43701..45683)
                     /locus_tag="U4A13_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN1778743.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            45863..46528
                     /locus_tag="U4A13_000416"
     CDS             45863..46528
                     /locus_tag="U4A13_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778744.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(48463..>50334)
                     /locus_tag="U4A13_000417"
     CDS             complement(48463..>50334)
                     /locus_tag="U4A13_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1778745.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT        14642 a        10366 c         8402 g        16924 t
ORIGIN      
        1 aaattcgcct aaaagttttc tgaatagttg taattgtctg aaaatttgtt atcattagtc
       61 attgttaaat attgctattt attcttaata taaattttga ttgtgaattt aaaagaaaga
      121 ggtcatttat ggaagataaa aaattgaaaa aacaacttag ctccagacat atcaccatgt
      181 tagctttagg tggagcaatc ggtgcaggtc tatttaaagg aagtggcgaa gcgattggga
      241 tcgccggccc ttctgtattg attgcttttt tgattggtgg gctgatttta tatatcgtga
      301 tgaaaggtct gggaaaaatc gttttaagcg gtggcgacac gcatcacgga ttatctggtt
      361 tgatacgccc ttatctaggt gctcattctg ctgattttac tgattgggtc tactggtcta
      421 tgtggattat caatatcatc gctgaagctg ttgcagctgc ttcctttttg cagttgtggt
      481 tcccgcacat tccagcctgg gtatttgttt ttatgttggc tgttttaact acgatcatca
      541 atctttattc ggtacggtta ttcgcagaaa cagaatattg gctggctttt gctaaaatca
      601 gtgtcattat cttactgatc atttttgcta cttatcttgt cggtcagcaa atgttgggca
      661 caggcgtttt ccctaccctt cagcaattga ctgaccatgg tgggtttact cctcatggta
      721 tgaagggaat catcagttct ttgcttgtag ttatctattc ttacggcggt tctgaactga
      781 ttgctatcac ggtaagcgaa gcagatgatc ctaaacgtgc gattcctaaa gcaatcaaag
      841 gtgtcatggg aagaatcatt tccttttaca tcattccttt gtttttgctt ttgattcttt
      901 ttccttggaa tactttagca ggcacgaatg tcagtccttt tgtgatggta tttgaaaaaa
      961 tgaatatccc atttgcagcg gatatcgtca attttgtgat tgtccttgct ttgttctcat
     1021 ctatcaattc gggtgtgtat gcatcttcaa gaattttgtt cttccgtttg aaggatcgaa
     1081 aaggttcctc tagaaaacta gctgttttaa acaagcatca agtaccgcaa cgtgctgttt
     1141 tattttgtac cagtgttctt tatcttggtg tagctctttc ttattttgtc ggtgacaaat
     1201 tatttggcta tctggcaggt tccctttcgt atacagtatt attgatctgg atcattatca
     1261 gtgcggcatc ctttgtactg gcactaagaa aaggaacatt atgggataaa ggaatcagct
     1321 tttttgcatt agctgtctta ggattgattt tccttggtat cctgttcacg aactccattg
     1381 gggtaactgt attgactggc ctgctttacc tgttcattta tttcagttat caaaaaaaga
     1441 atgatgcgtt cgtattgacg aatgaatgaa atcgatagaa aattttattt tatttatgtg
     1501 gaatagatgg agctattgaa gaaaggagtt gtgacaaaag tcttgttgca actccttttt
     1561 ccgaataaac agtctcatga cctcttggta aacggtgttc aaaaaccctc tgtgcaaaat
     1621 aattgattgt tctccatgct catgacatga tagaagaaag gaatgaaaaa aaggagtttg
     1681 ttttaaatga aaaagtattg gaagtttttg ttgatgatcg gtgtttcaac ggtagttatg
     1741 tttggtatga tgtacttgaa tgtctatgcg ctcgatcata tcttcttcag tcgtacacga
     1801 gtattcatgg cgttgatgat gggtgcagtc atggcagtca tcatgctgtt gttcatgtgg
     1861 aaaatgtatg aaaacaaaaa gataaatgtt atgatcttag ccgctagtct tcttgttttt
     1921 gccggttcct tgtttatggt acgaagccaa actactgtca acgacacagc ttggatgaag
     1981 gccatgattc ctcatcattc tatcgctatc ctggcaagta aacgagctga tttaaaagat
     2041 cctgaagtaa aaaaattagc ggaagaaatc attcaggcac aagaagaaga gatcgacaaa
     2101 atgaaaaaaa tgatcaaaga aatggaatga ccattatccg tccaaataaa aaaagaggat
     2161 gttccagaag ttatcagctt caagaaataa gaagaagcta cttctggaac gccgtgaata
     2221 aggggttgtg aaaaagctgc cctgcatcaa gtaataagaa cagccaaaca aaaatagctt
     2281 ctcctatttt tcgtttggtt aggcttatta ccgcagatgc aagcttttga acaccgttta
     2341 ttaagaggtt gtaaaatcag cgttctgacc tgagtattag agagacaaat ggaaaaatag
     2401 ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg aacaccgttt
     2461 atcgagaggt tgtgaaaaag tcgcttagtt tcgcgtatta agaaaaattt tgtgaaatgg
     2521 ctttccacat ttttgtataa atttggctta ataccgagaa actggctttt gaataccgtt
     2581 tattcgtaaa agggattgtg acaaaagtct tgtcacaatc cctttttatg tttttattgt
     2641 tctaattgcg ctgtcttgct tactgcttct tgcggtttca caaatttttt tgagattccc
     2701 caacctgtga aggcactgac catcgaaaac acaggagaaa gcaaactgaa aaatacgaat
     2761 ggcaagtaag tgagtgtagg aataccaaat gtacttgcta cgaaacttcc ggcaattccc
     2821 caaggcacta agtagttgat tactgttcca ccatcttcta gcgttctacc taatacagtt
     2881 ggatcaaatc ctgctttttc ataagtttct ttaaatgcat taccaggcag aataacagat
     2941 aggaattgtt caccaataaa aatatttact ccgatacttg tcatcaatgt tgtaaaaatc
     3001 aaatttggtg tgttcgtcat ctttcttgat actcgttcca tcacggttga gatcaagcca
     3061 aattcgatca acaatccacc taatgacaaa gttagaataa tcaatgacac agtaggcatc
     3121 atactttcga ttcctccgcg gctaagcagt gaatcgattt gttggtttcc agtatttgaa
     3181 acaaaccctg attcgatgac cccagccaac ttcgttaagg aagtttgtgg gttttgaata
     3241 aaaatcattc ctactgctac agcgatattc aacaaaatcg ttaaaattgc cggcaccttt
     3301 ttccatgcac acagtaacat taaaaataaa ggaatcagtg accagattga aatagtaaag
     3361 tttgcttcta gaacatcagt cacttcacga atagatgaaa gacttgcggc tttacttgtg
     3421 tgtcctaaaa tcatgaataa aaagaaagaa acaacaaatg ccgggacagt tgaccacatc
     3481 atatgcttga tgtgtttgaa caaatcggca tcaacaatag ctgctgctag atttgttgat
     3541 tcggataatg gagacatctt atctccaaaa acggcacctg aaacaatagc tccaacaacc
     3601 agcgctggat ttaagcctag tgttgtccca ataccgaaaa atgcaatccc aatcgttgac
     3661 ataacagtaa aggcactccc tacagcgcta cctacgattg cacacacaac aaatacagaa
     3721 gggacaaacc actggacact gatcaatttg aacccaatga ccattagagt tggaatgatc
     3781 cctgcttgta tccatacagc aatcaatgaa ccgactaaaa taaaaataaa gattgggatg
     3841 attcctggtt tgatgccatt gatgattcct tcgtgcagcc aatctgtcgg gtatcttttt
     3901 atcactgcat acaacatgac cattccgatc gctgctaaga tcgtgatatt aggtgagacc
     3961 tttaatccga caacgccaat cgcaatgcct aaaacaataa ccattaatac aagtgttgct
     4021 tctaaaaaac ttggtgttcc tttttttccc atgataatct ctcctttttt agttgttctt
     4081 atgttctaaa aaagttaaga tatcggatta ctccgccgca attcatccgc gattcctcct
     4141 ttataaaaat aaaaagacct gttttaaact aaggacgaat gactccgcgg taccacctta
     4201 attaaaaaca gatctgtttt tcactttcaa acttttattc aaatgtatgg actttccatg
     4261 taattcgtca attaggcttg atcggttcac agcgaccacc gactttctga acaaaacacc
     4321 taaccgctac tgcgtgaaaa gttgacttgt acgaagttta ccttccataa gatcatttgt
     4381 caatgaaaaa gaaaatattt tttcattttt ctgaaaatta tttttataac tttcacacac
     4441 aagtttattt tttactagaa agtagcgccc tcttgttttt ttcttgctac tatttcttga
     4501 tgttcttgct tgattctttc taatagcttt ggctctttca acagatcaag agctgtattg
     4561 gcgagtagtt ctgctgcaat cgcaatagaa gcaaggcctt tgtcactttt agcagcagct
     4621 ttgaattctt ctgtgtgtcc ggcaatatat gtctcagaga ttgctaccgt aggctggatt
     4681 gttggtatga cttggctgac atttcctaca tcagaagaac cgagacttgt tctttgttcc
     4741 gtttctattt cttcatctgg aacacctgct tcttttgcat gctggaagaa taagtcatca
     4801 aaagaaggag taacgatgac atcatcaact gcattttgga acaatccaaa ctcaaaagtc
     4861 gtgcccgtac ttatcgcagc acctcgtacg atattttcca ctttttcata tactttatct
     4921 aatgtttgcc gatttcctgc acgtaaatag aaacgtccag cagcgtaatc tggaatgacg
     4981 ttggcagcta ctcctccgtc tgtaatgatc ccatggatat tgacatcttt tggcaattgc
     5041 aagcgtagag cgttgattcc attaaacact tggatcaatg cttccaaagc atttactcct
     5101 ttttcaggtg ctgctgctgc atgggaagcg acaccgtaaa atttgatgtc gactggatca
     5161 ttcgctaaag attctgtcgt tttcccataa cgaaaagctg gatggacgca taacgctaca
     5221 tcgacatcct caaaaaaacc ttctcgaaca aagctgcctt tagctgatcc gttctcgccc
     5281 ccttcttccc ctggggtgcc atatacacgg atttctccac cagtttcatc aatgaattgc
     5341 ttcactatgc tggcggctaa tacggagtaa gtaccaaaca aattatgccc gcatgcatga
     5401 ccaatacctg gtaaagcatc gaattctgcc aaaaaggcaa ttgtgggacc gggtttattg
     5461 gaaatataac gtgcatcaaa ggctgttcgg tgtccagcta cttctttttc tacccaaaac
     5521 ctttcttttt ctaattgttt aatcaacgct gctgaggaat aaaactcata gttacttact
     5581 tcaggatgtt cgtggatatc tagagcaagt gcttgatatt ctggtaaacg atattggatt
     5641 agatccttca attggtttgt tgagattgtt gttgtcataa aaaattcctc ctatgctagt
     5701 catttttctt attttctccc gaatgtttcc catgcaggca cgcttgatcc ttttgatgtt
     5761 ttctcgatta ctttgactgt gtcttctgtt tgataagcgt ctactatttt ttgatacgtt
     5821 ttattgtttt catcttcttt tcttgcaacg atgatattca cataaggacg tgcatggtca
     5881 tccactggtt ctaagaagat ggcatcagaa gcaggattaa atccagcatc tacagctaca
     5941 ccgctattga tcactgaaat gtctacatct gacaaagcac gtgctgtttg ggaagcatct
     6001 aattcagaaa tgtctagatt ttttctattc tcggtaatat cagaaacagt gggcgcttgt
     6061 ccttttgcct tatccacttt gatcaatcca gcagtttgca ataacagaag agctctgccg
     6121 ccatttgtaa catcattagg gattgctact tttgctccgt ctttgatatc agctacatcc
     6181 ttcaaagtgt tagaataaat tcccagcggt gcattgacag tatttccgat cgatactaaa
     6241 tcagttccgt gttctttatt gtaattgtct aaaaagaatt gatgttggaa agaattcaaa
     6301 tcaatcgatc catctgctaa agccacattt ggttgattgt aatctgtaaa tttcacgtat
     6361 tccaaattaa tgttctcctt tttcaaacgg tctttcacgt tgtcccatac atctgtatct
     6421 tctccaataa tccctagttt tacgatagtt tcttttttgt cagttgtatc tgcttttcct
     6481 tggctacacc ctgccaacca aataattgct acaaatgtaa tgactacaaa acgaataatt
     6541 tttttcattt tttctcctcc actttcaatg tgtaattttt ttaatcagtt gattactgat
     6601 aaattgactt aaaaaaacta agattagaat caataatgta gccacaatcg ttacatctgt
     6661 ttggaatcga ttataccctc tcgatatagc aagattccct aatccgcctg cacctactgc
     6721 tccagccatg gtagtcagtc cgatcacgtt gatgatagtc aacgccgaga cccgaatgat
     6781 accaggcaaa ccttctaaca aatacacacg taaaatgata ccgattggac tggttcccat
     6841 cgcttctgcc gcttcgatca ccccaggatc aacttctagc agtgcgttct cgatttgacg
     6901 agcaaaaaac ggaacaactc caaaaatcaa tggcactaaa gctgctttcg caccaatcgt
     6961 cgttccaact aagaaacgag tagtcaatgc taaaagagaa agcaagataa tgaaagggat
     7021 ggaacgaatg atattgatca gcttatcaag tatttcaaat agcagacggt tagctagtaa
     7081 tccattaggg cgtgtcacta ctaacgtcac acctaagatg attccaatca tcccgccaat
     7141 gaatgctgtc caaaaagaca tatatagtgt ttgcttcgtc gcttcgacaa attcatccgg
     7201 aatcaaataa acattgggca aatatgtttt aaagaaatca gtcattttat cttcctcctt
     7261 cgatcacttt gatacgacta tctaaatcgt ttttttgtaa aatatttatt tttacaccag
     7321 atttgatcaa ataatccaat gcttttttcc gctgtttggt ttctccagct aaagtaacga
     7381 tcaaactccc taacggcgtt tcttgtaata actctacatt gccatataaa atattcgctg
     7441 ttacttgata tttgctgtac agatgagcga ttagaggttc gttcgtctga tttccaatat
     7501 aacttaattc caccagccat ttattggcga tctgatcggc aaaagcagaa tgggcaatga
     7561 tcgtttctaa tgcttggtca agatgagtag ctgttcgaat aaaatctttg gtcaattctt
     7621 cttcaggatt gctgaagatc tggatgctgc tacctttttc aacgattctt ccatcttcca
     7681 taaccgctac cttgttgcag atttctttga caacttgcat ctcatgagtg atcaggacga
     7741 ttgttaaacc taactggcga ttcaattttt ttaacaatgc aaggatctga agggtcgttt
     7801 tcggatcaag tgcactggtt gcttcatcac ataataatac cttaggttca ttcgccaaag
     7861 ctcgtgcgat tgctactcgt tgtttttgtc cccccgacag ttgtttagga taagcagatg
     7921 ctttttcttc caaaccgaca agttccagta gttcattcac tttttgacga cgttcctgtt
     7981 tcgattttcc agaatacttt aaagaaaaat caacgttgtc aaaaatagtt cggctttcca
     8041 ttagattgaa atgctgaaag atcataccga tggcctttct ttcttttcgc aattctttgg
     8101 gagagagctg actcagattc gtctgattga caatgacttc tcctgctgtt ggtttctgca
     8161 gcaaattcat cacacgaact aacgtacttt tccctgcacc tgagtaaccg acgattccat
     8221 aaacatcgcc tttttcaatc gttaaatcta cagaatctac tgcctgtatc tctttttctt
     8281 tttgtttgaa acatacagaa acatctcgca attcaatcat taaaattcct ccttatctaa
     8341 aaaaagcgtc ctcaagaaaa tgaaaatgtc attttcttga ggacgctttt ttgcgtgtta
     8401 ccacctcatg ttcaagctgt gttcacacac aactcctctt caagtacgat catactctag
     8461 caagatatcg gtcgcagccg tcatcgcctt acatctatgc tcgggatgaa ttgctcgatg
     8521 gccattttcc aatagtcgtt ccttgcttct tctcagctac cgaagctctc tgtcaaaggt
     8581 tcttctatcg tacttaccat ttcaatgcaa ataatattca aaaaaaaagt ccttttacta
     8641 acataaacta tgtcagtaaa aggacgacgt atcgcggtac cacctttttt cggaagtttc
     8701 cttccctcaa gcccaacatc tcaaaaagag agattggcgt tcgttttaac ggtcgaacag
     8761 ttccgaattt gcctacttat cgacaaatta ttctccaagg ccattttcag cttttcttac
     8821 ttgactcttc tcaccagcca gagcctctct gcaaagtttc caaacttact ttccttttca
     8881 acgaaatcga tattttgttg ttgggattaa tctacatgag gaaaactgaa aagtcaagta
     8941 cgaaattcaa attatttttt ctatatttat ttttctgttt ttttatttaa catttcaata
     9001 acagttcgaa gatcttttcc ttccgcaaga agtttcaatt tttccttttc ttcgacggta
     9061 taatttcgga caaaactgga atcaggtatc aaaacaacgc ctaatcctgc tcgtgaagca
     9121 gctgttgctc ctactaaaga atcttccact gcaaaggctg aactagcttc taatcctgtt
     9181 cgttccaaag ctgtcagata aggaatcggt gaaggcttgt tctccgacac ttcgtctccg
     9241 aatgtaaacg tgtccaagta atcagctaat ttaaagtgat ccaagatatc tgttgctctt
     9301 ttcgtaacag tggtcgtagc aatgcctagt ttcatatgac gttcttttaa gtctgccaat
     9361 atttcttttg catatggttt taagtggatg gctccgcttg tcagctgttg ataaaaatat
     9421 tcttcacgct tcgctcggac ttcctgtact ttttcagggc tacctgctgc tttcactaaa
     9481 atatcaaaac tttgtttcca gttttttccg ccccaagcgt caacgatttc ttttgggata
     9541 tggatatcaa attgctgaaa tccccaaagc cagccatcac gatacgtttt ttctgtgtca
     9601 actaacaaac catctaaatc aaatattgca cctttcatct atacattaat cctttccaaa
     9661 acaatacaca tagtttagca cgactggtcc aaaaagaaca gaagcaaaga accagccttc
     9721 tcgtttataa gagttcttgg aatcgacgga tatagatttt cttcacagct gtcacaagcg
     9781 ccagataagc gatgactgta gcaaataacc ataaccagta attgccagga agtggcatca
     9841 agcctaattc agcaccaaaa cttgtaaaag gaaggacaga accgacaata attcctaatg
     9901 tagtgataac aaacatggca aatgttgcgt tgctttggat aaatggtact ttcggtgtac
     9961 gcagtgcatg gagcactaag gtttgtgtcc atagtgattc gacaaaccat cctgcatgga
    10021 ataatgcgat gaaaatcact cgctgtgaac tatccaatgt atggaaactt ccgcccacta
    10081 cagcaggaca aataacaaaa tacatcaaca agtaagtagt aatatcaaat atcgaacttg
    10141 ttggaccaaa gtaaaccatg aattccccaa tactagatgc attccatttt tttggctcgt
    10201 ccaaatattc tttatccatc cgatcccatg gcaaggaaat gcaggaaaca tcatagatca
    10261 gattcaaaaa caaaagttgt aaaggcagca ttggtaaaaa tggcaggaac gtacttgcaa
    10321 tcagcacgga aaacatattg ccaaaattag aacttgctgt agccttgata tatttcatga
    10381 tattcccaaa agtttctcgt ccagaaagaa ggcctctttc caaaatcgtc aaatcttttt
    10441 ctaaaaggat cacgtctgca gattctttcg caatatccac agctgtatct acagaaatac
    10501 cgatatcagc cactttcatt gcaggagcgt cattgatacc atctcctaaa aatccgacag
    10561 tatgaccatt tttacgtaaa gctgtcgtca aacgtgcttt ttgctgagga ttcaatttta
    10621 cgaatacttc atggtcttca gctacttgtg ctaattcatg atcactcatt tccgagattt
    10681 tctcaccggt aaccaattca ttgatctcta gacctacttg acggcacact gaacgtgtaa
    10741 ctaattcgtt gtccccagtc agaactttta cagctacacc atgttctttc agagctttca
    10801 acgcttgttt cgttgtttct tttggtggat caaggaaagc aagataacca atcaaaacca
    10861 tgtctgactc atctttgact gaaaattctc caacgacgct tggattggtc ttttgtgcga
    10921 caccaatcac tcgtaagcca tcttcattaa gtgactgtac ttttgcaagt acggcttctt
    10981 ttcgttcttt tgttaatggt gtaacagtac cgccaagatc aataaattta gaaatgttca
    11041 gcatttcttc aatcgcgcct tttgtgatca tttgtgtttt accagctgga tcagcaacca
    11101 caacactcat acgacgacgt tcaaaatcaa aaggaatttc atccactttt tgataattga
    11161 tcttatctgt atctaaagtt tctgttgctt catcaatgat agccacatct aacaaatttt
    11221 tcagccctgt ttgatagtag ctatttagat acgcatggcg caaaacacga tcatcttcat
    11281 ttccttcaca atccatatga tattcaagaa tgattttatc ttgtgtcaat gtccccgttt
    11341 tatccgtaca taacacatcg attgcaccaa agttctggat ggcattcaaa tttttgatca
    11401 ctgttccttt tttggccatc acacttgctc ctttgaccaa attcgtcgta acgatcattg
    11461 gaagcatttc aggtgttaga ccaactgcta cagatagtcc aaaaagaaaa gcttctagcc
    11521 aatccccttt tgtaagccca ttgatgacga taactgctgg agccatcaat gacataaatt
    11581 tgatcagtag catggatgtt ttatggatgc ctaattcaaa gcttgattga attggttttt
    11641 cagatagcgt ttctgctaca tgaccaaata gtgtttcatt tccaacagaa acaatgattc
    11701 cttccgcact tccactgatg acattacttc ccataaagac aagattttcg taacttgttt
    11761 cgctttgctg atcgtcttca catactttcg catgcttttc tactggatag ctttcccctg
    11821 tcaatgctgc ttgggaaatg aataaatcct ttgcggacaa taaacgcata tccgctggta
    11881 tcatatcccc tgctgaaagc cgaacaagat ctccacagac gatttcttcc ataggaatct
    11941 ctgtgtactt tccattacgt tttacagctg ccgttacttt cacaagtgat ttcagttttt
    12001 cagccgcttg attggaacgt acggactgaa tcaacgtcat tgtcccgctg acgataacca
    12061 tagtgaagat aatggctaca ccgaaaagat ctttatctcc aggagctgct aatacatatt
    12121 ccgtgatgaa tgaaataatt cctaatgcga tcaacactaa tgtaaatggc gtgatgtatg
    12181 ctttcaatat ttctacaatg aatggtgttt ttttgccata tgaaatcgca ttttcaccgt
    12241 aattttctct tgaacgttcg atttgttctt ctgtcaatcc atttgctgat gcatggaata
    12301 gttcaaataa ttcttgtttc gtttttccag caaaagctgc atagattttt tgtgtatctt
    12361 tctttttttc catctcaatc tctcctttgt gtccaaaaga cagctgttct cgctgctttt
    12421 ggtctttagc tattcatcgg atcgatagag aaaaaactga aaggggctga gaacagatag
    12481 ataatagccc tggcctttcc tctagatcct tgtttttcga atgactgtca tccatttgtt
    12541 ctcatctcct ttactaagta attttaattc atgacttcca atcatttttc gaatagttat
    12601 ttttccaatt agaccacctc ctttttattt ttcaccgatg gggatttgcg gcaacaaaaa
    12661 aacctctcgc caaatactca gcgaaaggtt tgaaaaagca tattaaaaaa agcttctcag
    12721 atctcatcgt tgagttttga cactgtacaa cgtaaaaagc agatacctgc ttttagctac
    12781 cttatgaaaa gccttatctc gacaattcct gttctaccca ttggcgtctc tcgacgtttt
    12841 tgggcagtag cctatgtttg tataggagcc tcacctaaca aatcttattc ggttttcctt
    12901 cgttaatata acaactaaaa aagaggatgt caaatcaatt ttccatgttt ttcatacttt
    12961 ttttaaaata cacacgttat attcctgtct cacgcggttt tatttgtctc attttccacc
    13021 tcccgctata atagaaatat agtaggaaac gaggtgctgt tatggaaaga acacttgtta
    13081 ttatcaagcc agacggcgtg agacgccatt tagtcggaag catcatccaa cgttttgaag
    13141 caaagggact ggccattgct gaaatgaagt ttgaaactat gactcctgaa ttagcaaaag
    13201 aacattatca gcacttgact gagcgcagtt tttttgatga actgatcgat tacatgactt
    13261 ccggtcctgt cgtttatctt gtgctggtcg gcgaggaagt gatcgatatc gtccgtaaga
    13321 tggttggagc aacaaaagct gccgatgctg ttccaggtac gatccgagga gactacgcgc
    13381 tgccggggac tgaaaacatc attcacgctt ctgattcccg tgatgctgcg gtaaaagaga
    13441 tcgcccgctt tttcccgcca ttcgatataa acaaaccgtc tgaaaaagcg gcgaagtgat
    13501 acaaattcta aactgctggt ctagttgctg ggttcagcaa tggccagcag ttttttctct
    13561 aaggacctcc gttgattctt tcctaacata tattcgagca ctgtgaagaa ttcttggtag
    13621 tctttcgtct tttcttcccc taaagcagca gaaatcaatc tttgataata atctgccatc
    13681 aatgtttcat agatcactgt acttctttct ttatgtgcta agctccgttt catatcttca
    13741 ttcacactac gtgccttttt caaccgacca ttttctatat aaaacgaagt caggttcatt
    13801 aaaaaagtaa agagatactg ctcatgattg ttcaactgtg ctaatcgtcg cattcttttt
    13861 ttgattttca aataattgtt ctcaatgaac cgatcactga atatccaagg aaaatcagct
    13921 agaatagata gttcaaattg accatagtct ggtatctgca tcaagtattc ttcaatgatt
    13981 cggcattcat aacgtaaaaa ttcaaattct gtctcagata cttctgtttt ttcgaccatg
    14041 tgaagattca aaaccaaata atacagatct tctgttttct taaaatgcct acggttattg
    14101 ctgatctctt tataaggatc aaagctgtct tcttgtaccc gatctagcat tcgtcttttt
    14161 ttctttaact ttattggttc atccagctgg tttcgcatcc attcatattc tgcaagtgtc
    14221 atgttcattc gctctaaaaa tgatctcaat gttacaaatg acattttttt cactgaattt
    14281 gtctcaatcc cgactagaga agtcttaaaa cagattcctt ccgctaattg ggactgtgtc
    14341 aacttcttgt tgactcgaag tttcttgatc aattcaccat catacataat agttctcctt
    14401 tttaatttaa atttttccac attcaataaa tagatacgtt aaaaaggaaa aatacacgaa
    14461 aaaaataaaa aaatgttttt ttcataaaaa ataccaacct tcaagcatta gaatgttgtc
    14521 taactactga aggttggttt gattgtttat tgattatttt tttgctgttc aacgtctgtt
    14581 cttgttactg gatataagtt ggttgttgtt ttattaccat tttttgaaaa gacacttgtc
    14641 gaatgttcgc ctttttgctt ttcgattgct tcattacgtt ccgcttcatt tttatagtca
    14701 tattttttcg gatcaatcgt ctcaaatccg tttggcacat aaaaccgcaa gagattttcc
    14761 tgattcagct tatctgaaag acttaacgca gtcttgactt tttcctgatc tttggagatt
    14821 tcctcatcga ttcctgctgc tttcgtatcg atgatttctc ctgtctgatt attgtaagct
    14881 tttcctccta ggacggtgta tttgggtgtc acaaagttcc catttcgaaa agcaactact
    14941 tgatcatgct gagaagataa taagtctgta ccaaaatgaa gataatcttt atcatcgatt
    15001 cccaatagat gtagtaatgt cggcaaaaca tcgatttctc caccgtattc atgattgact
    15061 gtccctttag tataaccagg catatggatc atcaatggca ctcgttgcat ctgtgcgttg
    15121 tcaaattcat cccatgtatc tggatcttta ccaagagcgc ttgctaaatc tttattatct
    15181 gtattcgaga tcccaaaatg atccccgtaa atcacaaaga ttgaattttg gtaaatacca
    15241 gattttttca aatacgtaaa aaattgttcc aatgattgat ccagataatg tgccgttcga
    15301 acataattat ctactgtgct attgccagta ttcaaagatg gaaagtcgaa attcttgtca
    15361 tccgtataat aaggcgtgtg gttggttacg gaaagaaatt tcgtgtaaaa cggctgttgt
    15421 agatgctcaa gatattgggc agattctctg aataaatctt tatctaatat cccataacct
    15481 aacgtttcgt cggattcatc aaaataagat cgatcaaaga aattgtcata acctaagttt
    15541 ttatatacat gatctctatt ccaaaaacta gctacattcc catggaaaac agcgcttgta
    15601 tagccttgct tttgtccaag gatcgctggt gcggcttgaa agacattgtc tgacccaagt
    15661 tctgtgaata atgagccttg aggtaaacca aatgtgccag tttccaacat attttcagca
    15721 tcacttgtct ttccctgtcc tacttcatga aagaaattgt caaagctgat cgttgcttga
    15781 ttctggaaaa tactatttaa aaacggtgtg acttcttgtc cgtctacctt catattaatc
    15841 aaaaactgct ggaaactttc taaatggaga acgatcacat ttttcccttt agcgatccca
    15901 aatgtttcgg gatttggctc tgcataatgt ttctttgtat aatctaaaac ttcatcaatc
    15961 ccattactgc ttgcatgcgc gcgaacactg ctagtcattc ctgttttgat tccgtcataa
    16021 atagtatacg catcgattcc tagatatttg acaatatatg aacggtcaaa tgttcgctgc
    16081 agcaattgtg gacggttggc ttcgctcagc gctagatttc ctgaaaaaat aagccctgaa
    16141 agacaggtaa cagcgatagc catcggtttg ccgacagggt tgcttttgat aggtattttc
    16201 ttgaccttca accaaaccag tagcccaatg aacacagcga tatccagcca gtagatgaca
    16261 tcatggggct tcatcaaaga aaaggagctt cctgacaatc cttgcgacac tttcgaaaag
    16321 cctaatacag atttgactgt aatgaaatct gtgaattccc gataaaaaat aatattcaag
    16381 tacaacaaaa gcgtatttaa aatatcaatg atcaaaagaa tagctaacgc tggctttggc
    16441 tttttaatat acaaagaaag gccaaaaaac aataaggtag tcgcaattgg gttgatccac
    16501 aagataaagt actggatcgt accttccaca cctaatgaaa aatctgcata gtaggcaatg
    16561 atcgtcttca gccagaaaaa gaaaacagct gtcaaaacga ttgctaaacg attttggcac
    16621 aattttttaa gttgtgtcac gtatatcact tctctctatt cacatttttt cttattttgc
    16681 tcctgaaaaa tgaaaatttc ctaaaaaagt tgttatctta agattttttt attctttcta
    16741 aagattattc cgtccacttt tcacagcata aaaccgctgc gatgtaagcg attaaactaa
    16801 tgaagagata aaagtcttgt ggtgaccgct tcactccacg gtattcaaaa gccagtttct
    16861 cggtattcgt acaaaaaaag aggctgtgac aaaattcttg tcgcagctcc ttatttcttg
    16921 atgcaggaca gctttttcac aacttcttcc ttaaacggtg ttctcctgcc aactcctcgg
    16981 ccacaagtca caatatttat aaaacatgag aagctgtttt ttgaaattgt tccttgtggc
    17041 tcagagttaa acggcaggct caagacctct tgatacacgg tgttcaatca gctgaccctc
    17101 ggtattagat tatgtcagga cgctaatttt acaacctctt ggtaaacggt gttcaaaagc
    17161 ttgcatctgc ggtaataagc ccaaccaaac gaaaaatata aggagctatt ttttgaaatt
    17221 gctccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    17281 cagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
    17341 gtttctctaa tactcaggtc aaaacgctga tttcacaacc tctgtttttc attgtccata
    17401 ggattgatat tgtccttgtg atgactcatt cgtaataatt gaggcttgat tttcttcact
    17461 tcccgttatc gtttgtccac tgccagaatc tgaaggactt gttcctgtcg actcgttttt
    17521 ttgagaagag acagttgttt tcttgacttg ttcatttgtc tttttccatt ccgctaaacg
    17581 atcggcgacc acttttttgt ctactttttc agaataagcg gtgatcactt ttccattttt
    17641 caaacgggct actttgtcgc catcatgagt caaaagcaaa acggtcaaat gcttgtcttt
    17701 aaattgatat tgtctgagag aaatcgcact aagtacgtca tcgatattaa taaattgatt
    17761 ttcttctttt ccttttggca aataaatgtc caattcgcct ttttcgtaat tggcagttat
    17821 tttatactcc gcccaccaat aagttgagaa ttcttttttg aaattatcta ctgattcttt
    17881 gcttgctacg attttttctg tttctttctg agcaagatct gctgtcattt ttcctgaaaa
    17941 tgctgcggat aaaataagca gtaagaaaaa accgaataac caaaagagaa gaaacggtct
    18001 ttttttcctc atgctttcct ccactcctat ttaattttga ctattctatt atatacaaaa
    18061 aatgaaatag aaagacaaaa aagttgaaaa agatcgtaaa taattgaact tctttttcac
    18121 aaaatggtaa gaatggagaa tttcacgcat ttgctctcaa ctattctttc ttaagcctca
    18181 aatccgtttt tatctgatac gtctttgaac caatgacccg attttttgat tgttcttttt
    18241 ccatcttgtg ctaagtctac tgaaataaag ccatatcgat tcttatatgc attggtccac
    18301 gaccagttat ccatacatgt ccacatatga tagcctaaac aattggcacc ttcttgaatg
    18361 gcttgatgga catatttcag atgagattgg acaaattcga tccgataatc atcgtggatc
    18421 atgccatctt ctttgataaa tcgtgcttcg ttttctacac ccataccgtt ttcagaaata
    18481 aagcagtcga tatttccata atgatctcgt acattgatca aaatatcata gattccttgt
    18541 tcatagatct cccatccgcg ataaggattc attttacgac caggcatttc ataattgtcg
    18601 aaataatcct caggcattgg accgttttta gtatcgattg gtgtttcttt tgcttttacc
    18661 cggcgtggct ggtaataatt gatacctaaa agatctactg tattttcttt gataaccgtc
    18721 aaatcctctg catggatcac aggcatgaga tcaagttctt ttactatctc aatcaattca
    18781 cttggaaact cgccttttac agctggatca aggaatgaac gattgaagaa cgcatctgct
    18841 atttgcgctg ctttaacatc ttcagggttt gattcgtctc ttggatagct tggtgtcaga
    18901 ttcaagatga taccaataac accaccttgc tccatttcat ggtaagattt gatggctaac
    18961 gctgaagcca acgcttcatg atagccaact tggatcgctt ttttcatatc tgactcttct
    19021 ggataatgcc atcgatataa ataacctgct tcgactggaa cgattggttc attatgagta
    19081 aaccattttt ttactttcga accaaacaat tcaaaacaac ttttggcata tttttcatag
    19141 gcttcaaccg tttctcggtt caaccatccg cctttttctt gcagatacat cggcatatcg
    19201 aaatgataca gattcacaaa gggttcgata ccgttttcaa tcaactcatc aaaataacga
    19261 tgatagaaac gaacggcttc ttcgttgatt gttccagatt catcaggtat caaacggctc
    19321 cattggatag atgttcgaaa tgaattatgc ccagtctgtt tcatcaactg tacatcctct
    19381 tgatacttcg tataaacttg cgaggttttt tcaggtccta cttgatcaaa gaacttttgc
    19441 ggttcttttt tgtaccaata atcaaaaatg ttctctcctt ttccatcgcc tgaaatcctt
    19501 ccttctgttt gtgggccact tgctgcagaa ccccaccaaa aatttgctgg aaattgatat
    19561 tttttcatca aagcacctct ttttcttttt tagttcgttt tagttgattc catgtatgat
    19621 attttccatc acaatcactt tttcattttc ttcctcttct tttttgattc gttttaacag
    19681 ctgttctgct gctcgttccc cgattttatc tgttggtggc gtgatcgtga ttttcggaag
    19741 gatactcaga tcgtttttat ctaaataatc atagtgactg aacgcaatat ccttcccgat
    19801 tctgacacct tcttgattga aacattcgat ggcaccaatc gacatattgt aattgctgac
    19861 aaaaatagca gttgcccctt tttctaataa tgcttttgcc ccgtaatagc cgctttttct
    19921 agaatagtct cctgtaaaga cctctaattg gatcgatgga tcattttgga ccgcttcttt
    19981 ggctcctgct aaacgttctc ttgctacgta gtctgtttga gtggcagcaa taaatccgat
    20041 ttttttatgt ccttgttcac catgatgagc gatcactttt gctacacttt ctcgatcatt
    20101 cactaatatt gtatcaatac ctgctggtcc ttctggacaa ttcaatgcga taactggaat
    20161 actcattttt gtgagcattt caaatccaga ccactcttct tcggataaga agacaatcag
    20221 cccatcgatc acatgcttca tcaggtattc aattttttgt tcaatttttt ctggctgatc
    20281 ggcaaacccg cttaataata agctataccc attttcttca gctactcttt cgatccctga
    20341 gacaacttcc gacccaaaac ggctggaaat cccgtttatc aaaagtccaa tagagaagct
    20401 tcgattattc ttcaaccctt tagcaatgat gttttcttta tagtccaacg ctgaaatcgc
    20461 ttctgctatt tttttcatgt tcttttcctt cagttgctga ccattcaagt aacgggaaac
    20521 tgttcccact gaaacaccag ccatcttagc cacatcacgg atcgatgcca tacattctca
    20581 ctcctgccag ttacatttga tttctctttc ttgttgttcg attatgatga ccgcattctt
    20641 tgcatagtgt tgcttgtcat caatatccat ataactaaag tcttcagtag agcaacttcc
    20701 tctcactatt aattatactt aaacaagtat ttgaaccggt tcatttttaa tatagcattt
    20761 ttttaaaaga ttgcaacact tttttaaaac gctttctgaa agtggcggat tttttgcctt
    20821 tttaaaattt tattctaaaa tttactctat ttgtgggtgt tttgtgcgtt tttctcttcc
    20881 atccgtttga agttttacat tccaatggta taatttcttt atcctatcaa atgggggaag
    20941 tattttgaag aaattaatcg tactatttat tttgtctgcg ggacttttga cattgactgg
    21001 ctgtgatttg tttggcggct ctgaccaatc ctcggacgaa acaaccgatt cttctgtctt
    21061 aaaagcggaa aaagcagata ccaccaacag tgtatatacg ctaaaagtac gtaaagatat
    21121 ggatttatcc gcgggacagt tgacatttac agataaaaaa atggaatgga cacgcacgta
    21181 tcagacacaa ggaactgatt cttctgaatc atctgaagaa aaaacagtgc tgacagatat
    21241 caagatcact accaaagatg acgaatatat tatttcagga aaagaaaata aaaaagaaac
    21301 aacagtcata tttacaaaaa tcggtaatta ccgtttgaag gatgaggatg gcaacatcta
    21361 cagtctatag tgccgtctgt tggatcttct tttgtttctc cccttctttt ctgctatagt
    21421 aaagaagcaa gatgattgta aaggagacga aatggatgat cgatagtcct gattattgta
    21481 catgtaataa caacaaaaat atccatacag aatttaatga ccaaggaagc ttgagcatat
    21541 gcgacaactg tcacaaacta gtcaaagatt catttaaaac atttgaatct tatcctgttc
    21601 aaaattatgt aaaaagtaga agaagataaa caaatccaag caaaactcac tatggctgat
    21661 ttttatgaac ttcaaactca gcttcttcct ttgatcaagc ttcatcagta aaacaaacag
    21721 tggatcattt cagtttacaa atcaaagagt aaagatttat ttatttcaaa tatttgaata
    21781 taaaataacc aaacgataga aaggcttatt ctacatcacg tagtaatttt tctttcccgt
    21841 cgtttggttt tttattttta gcctaattct tgaccttttg catgctggtt atccgtaaaa
    21901 tattttccag caggctttcc ttccaattca gctaattttt cttggatctg acggatcacc
    21961 ttttttgcat cttctggatt ttctacaaaa tcgaatttat ccccatccac ttgtatttta
    22021 ggacatacat caaaatcttc ataccattga ttatagcggg tgttcaacgt ttcataatag
    22081 gtatataacg tctcatcgct ttctagctgt tcatagtcac gcccacgttt tttgatacgt
    22141 tctagcatcg tgtccaaaga aactttgata tggaccatca aatcaggccg tttttttgga
    22201 gcaacatcat ctaactcgtt tagcatcgta tctaataagc tatcgtactg ctgtacttcg
    22261 atatccgtta cccgacctaa atcagcgttt agatggaaca acaggctatc ttcatagatg
    22321 gatcggtcta gcacattgtc atcgttcact aaagcatttt tcattgctaa aaatcgcttg
    22381 ttcaaaaaga agatttgcaa taaaaaagcg tactgctcag gatttgaata aaagagcggc
    22441 aatacttcgt tatcttcgac attttcataa aatggtttag aatcaatctc ttttgcaagc
    22501 atatccgtca aactgctctt acctgcacca atcgttcctg aaatcaaaat cactgacata
    22561 caacccttcc tcttctataa gtactcgcct acgtaagttt caacaggcaa tgtttcaaaa
    22621 tcatattcac gtacagcttg gtctgatttt aaatacgtat ggagactttg tgtaatcatc
    22681 aagttcgtag gtaccgtctc ttcttgtaca atgatcatcg gttttttcaa catagttgcc
    22741 acacctaatt cgtaagccgt tcctggatcg attgtttgat gctcaaagtc aaggatagca
    22801 acaacaaatt ctgcatttgt cagttcttcc atatcttttt cgtatacttc ctgcgcccat
    22861 ccagcgctga atagttcata attgctttct tggtgatgac gtggagagta gaaacttgtg
    22921 actgttttgt tttcctccaa cgctttttcg atacgactta ctcgatcgat ttgctcctct
    22981 gaaaaaaatg gtcctgctaa atatatttga ctcattattt tgcctccttc aataataact
    23041 gatttttaat agatactgct cgtaattcat tgaccagttc caatggattg ttggaaaaga
    23101 ttacttgatc caaccatttt ttatccataa aatcctcttt tgcaaaagtt tctaataatt
    23161 gttgcaagcc gctgtaataa ccgccaatat tcaatattcc gattggcttg tcgggcgtca
    23221 gaccgattgc tatccaagaa agaagttgtg agaattcctc caatgtacct agacctccag
    23281 ggaaaacgag gacagcatct cccttttcaa ataataatac ctttctttca tcgatcgttt
    23341 ctgtaggaat aaatgtagta acatcttttc caggcaattc agtttcaaaa agcccattcg
    23401 gatagatacc tgtgactttt ccgttatttt ccagtactgt tttactaatc actcccataa
    23461 tcccggagta tgaaccaccg taaaccagtt cgatttcttc ttgtgccatg tattttccta
    23521 acgtttgcgc aataaatttg taactttctt tattaccaaa acgggaaccg caaaaaacag
    23581 taagtttcat aaaaaaacac tccctaactc aaaagtctat cttcattcta cacgtgtttt
    23641 tttaaaataa aagactagat tttgtagttt tttagtggta tataggatag acgccactat
    23701 atatagtatt tagaaaaaaa tcatttttca gccattttat agataaaaca aaaataaata
    23761 acgtgttttc atcgatatga aatgacttac ttatagaaga tactgggatt tcgtcctata
    23821 tgccaataac gtgtattatt ttaaacaaca aatacttgtt tcttttatta acgaaaaaat
    23881 cattttgttc tcgtgttatg tttcactgat aaatgatgtt tctctaaata aaaaagccga
    23941 cgaactaaac gatcaaaatt tgatcatttt tgttcatcag cttctcctaa aaatcttttt
    24001 gttaacgata aatagctgga tttgtaaaaa tatcacttga atcgtcactg ctggaagaaa
    24061 attcagtgat aaccgcacct gttgcgcctg cctggaacta gtgtttgata ttgggctcga
    24121 acgtgtactg atctctagca tgaagaataa tttctttttt tactgtataa taactttctt
    24181 tcctttttgg aatagtacag gaaagttttt ttgtttcctt tccttcgaca taaggtatac
    24241 cagacccttt cgacaacgaa aggttctcat ttttccttca tcgtcatttc aaggagggtg
    24301 ccgatgctct ggacagtttc gatcgctcct aaaccaaaat ctgtatattc gattctttct
    24361 aactctgatt ctttcatacc attcctgtct caaggtacat ctattgtact tttttcttgt
    24421 aatccattct gcctctccta actaattgtc aaatcatccc aagatatgtc ttcttcctct
    24481 ttttcttctt ggatgatttc tgcattcgga tctactggtt ttttcaataa taaaatggtt
    24541 attcctgtga caaaagaacc agctaacaat gcaccaatga atgcccatgg tctggtcatc
    24601 gtagcgactg caaacattcc cgatgccgga atacggctat ctgcgcccca aaacatgctt
    24661 aatccgccac ctactgtccc accgactccc gttgctatga cattgcgtgc caagtcgttc
    24721 aatacgatag ggaaacatcc ctctgtgatc tgacagatcc ccataggaaa agcggtcttc
    24781 aatgtatcga tttcttgacg attgagtata tttttacgaa tgattttccc taagaaataa
    24841 gcgatcgtca agccgaatgg ggttaccatc gaagctaaaa tcaatacagt gattggttca
    24901 tagattcctt ctgaaaatcg tgccacaccc ccttatccag aataaatggt aatccagaaa
    24961 cagccgtgta tctgcggtaa taagcctaac caaacgaaaa atatgagaag ctatttttgt
    25021 ttggctgttc ttattacttg atgcagggca gctttttcac aacctctttt tttatttatg
    25081 tttcataatc aatatggtaa ggatgcagct ccgtatggta gaccgcctcc gaatattcaa
    25141 tcatttcccc aatttgcgta gacgatttcc gtatccgttt gatgccgatc tgtgcgtctg
    25201 ttttcagaat tccttgttct tcttctgtta aaaatactgc cgagaaatca tcagaaaatg
    25261 agtcaatagt atatcctttt ttcgtcagca aacgatacaa agattctttg ccaaattctt
    25321 ctctgctgaa ttgcatcatt tcttgtggaa gataatgaac aaaataaata taaggctgat
    25381 tgttcaaata atataagcgc ttaaaacaaa cagcttgctc accgaaaaag gaaaacaaag
    25441 gatcttcctt ctttatatgg accaattgca aatccagcgt ttctttccgg acttcatatc
    25501 gggagttctc caagatttgc gtaaagcttg ccgctttgga taatcgattg taaggacgat
    25561 tgcttagaac cgtcgtccca cgaccacttt tctttgcgac atattcatct gatgccagca
    25621 gttcgattgc ctttcggaca gtgattttgc tgacttgaaa cagttcttct aattcacttt
    25681 ctgtcggcaa catcgaaccg actggatatt ttcctgagaa aatatcttct ttaattttat
    25741 ctgcgacttc caagtatagt acacgtgtcc ttttcataac gtcacccttt gctttactat
    25801 tactaaaaga atagcacata aaaaataaaa aaagaacaca aatcacatgt gagaagtgtg
    25861 ttctttttca ttcagctgct ttaattacaa gtttacgcca tttgaggcaa ttacttcttt
    25921 ataccaatca aaagattttt tctttgatcg attcagtgtc ccatttcctt catcgtctag
    25981 atccacatag ataaagccgt aacgtttact catttctcct gttgatgcac tgacaagatc
    26041 gatacatccc caaggcgtat agcccatcaa atctacaccg tctgctactg cattagccat
    26101 cgcttcgata tgacgacgta aataatcgat ccgataatca tcaataatcg tgccatcctc
    26161 ttccatatga tcaatggctc caagaccgtt ttcaacaatg aataatggtt tctggtaacg
    26221 attgtataat tcattcaatg cgattctcag cccttctgga tcgatctgcc agccccattc
    26281 acttgcctca agaaacggat tttttactcc tcccagcaag ttcccttctg aagttgctgc
    26341 ttgtggatca gtctcattga ttgccgtaga catataataa ctaaatccga tgtagtccac
    26401 tggatattct tttagcaact ggaagtcttt ttccgtttgc tctaattttt ccggatcgac
    26461 tccatatttt ttatgggtac gcgctgtgta tgcagggtat tctccacgta cttggacatc
    26521 cgtgcagaaa aaattgaatt cttggttttg gatcattgtt gctgcctgat tgactggatt
    26581 ggcatcgatg ctgtaagttg ttgcatagat aatcatgcag ccgatttgga tatccgaacg
    26641 caattcgtgg ccgattttta cggctttgct acttgcaacg aattgattat gccatgcttg
    26701 gaaaatattt tggtactctc ctgctccatt gctttttact aatccttgac ttaaagctgg
    26761 aaagtgaaag gccgaattga tttcattgaa agtcatccaa tatttgactt tactgctgta
    26821 tctctctaat actgtctgag caaatcgttc atagaagtcg atcagttttc gatttttcca
    26881 gccaccgtat tcttttgcca aatgaagagg catttcgtaa tgagaaatcg tgatcacagg
    26941 ttcaatatca tattttaagc actcatcaat cagctgatca taaaattcta atcccgcttc
    27001 gttaggagta ccttcgtctc catttggaaa aattcgtgtc caggcaatcg aaaaacgata
    27061 acatttgaat cccatttccg caaacaaggc aagatcttct ttgaaacgat cgtaatgatc
    27121 gattcctcga tgattcgggt aaatatattt ttcttgatca atcgtccagt caaattcttc
    27181 actgccgata atcgcaaagc gctgcttacc tccaggcata gcatctgcaa cggatagtcc
    27241 ttttccgtct cgatcataag ccccttctaa ttgatttggc agcagttgct ccaccccata
    27301 aaaatttttc tgaaaaacgt gattgcatcc tgatttcctc cttattttac aattgccaga
    27361 acatcttcgt ttgcaataat ctctttttgg tctaattcaa gtatatcggc aaatgcatca
    27421 gtattcgtaa tgacaactgg agtgattacg ctatagccag ctgctctaat tgcttcgatg
    27481 tcaaaacgaa tcaataaatc tcctgctttg acgagatccc cttgtttgac taagatctca
    27541 aagccattcc catttaattc tactgtatcc atcccgatat gaatcaaaat ctccgctccg
    27601 tcagtcgttg tgatccctac agcatgtcca gttggaaaaa tagtcgtgat ctctccgtct
    27661 gcaggtgcaa ctagttctcc tacttctgga tcaatagcga taccttttcc catcacgcca
    27721 gaagaaaaaa cttggtctgg tacttcactc aatggcagta ctttacccgt taaaggactt
    27781 tttaaggtat gccgtgctga agtaatcggt tttcctgcat tcgcatgttt gttttctaat
    27841 tgattgtctt gtgtttgttc gtcaaatcct aaaatcaatg tacctacgaa tgcaagaaca
    27901 aaagcaattc ctgtagctat tactgctact gtcacatttg actctacacc atccaccgta
    27961 ttaatgaaag tcggaatcgt caataagctg accaaactgc tggagaaagc ttttactccg
    28021 gaaaatccga taatagctcc accaatacct ccagaaatac aagcggcaat gaatggtttc
    28081 ttcaaaggta atgtcacacc atacacagtt ggttcagtga ttccaaataa cgaagtcact
    28141 gtagaagaaa tccctaatgc tcgaagtttc gtatcttttg ttcgcaatgc tacagctaaa
    28201 gcagccccac cttgtgccaa gatggctgga agaagcattg gcatcaatac atcaaaccca
    28261 tattgttcga tattcaaaaa catgatcggt acgaatcccc aatgcatgcc gaacatcacg
    28321 aatacttgcc ataaacttcc catgattaga cctgctacga ttggactaaa tccataaatc
    28381 gagttgaaga gaccgcctaa agcattaccg gcaaccgtcc cgattggtcc taaagcaaga
    28441 aatgttaatg gaaccatcac tactaaaacg acaagcggaa ctaaaatcat ttgcagaaat
    28501 tgcggaatca ctttttttac gaatggctct aatttacttt gaacccatac ggctagaatg
    28561 attggtatga cagaagaagt ataaccagat ggtgaaagaa tcactgggat cccaaagaaa
    28621 ctgatagctt ctcccgcacc agcaactgct gtgatagctg gatacacaag tgccatagca
    28681 attgctactg ctaaaaactg gttggctttg aactttttag ctgctgtaaa agcaagcatg
    28741 acaggtaaga aggtaaagac accatctgct gcagcaaaca atatttgata agtaccgcta
    28801 tcagctgtca gccagcccat caccgttgcc aatgataaga atcctttcaa taccccggcg
    28861 gctgccatcg cccctagaaa aggtgtaaat atactagaga taatatcgat cagacgattc
    28921 aaaatatttt tggactcttc ttttccatcc tccgaaactg caagaccttc ctcatccacg
    28981 atcgattgat aaacatctgc tacattactg ccaatcacta cttgatactg accgccactt
    29041 tgtacgactt ggatcacgtc cgggtcttct tttaactttt gcgtatctgc taaagattca
    29101 tcttttaatc tgaatcgcaa ccgcgtagca caatgtacta aacttttgac attcttttgt
    29161 cctcctaccg cttcccatac acgacgacca actgcttgat tatccattgt tttttccttc
    29221 tttctttttt atttaaaaaa taaaaaccca gattgaaaaa aacagtcaca aacattcgtg
    29281 aacctatttt ttcaatccag gttttgcctg ccatacagta acaatcctta tgattggatg
    29341 ctttttttct cacttactac tctttgaata tgaatcgtta aataaatctg ctctgcgatc
    29401 cccatttcct gtttttgcgt ttgtcgaaga tactcattga tccgttcgac acaatgaaac
    29461 gcttttggat actttccttg aactaatgcg tagagaaatt catcttgctc gtcagatcct
    29521 tcccctttga gataacgctg ggcaaaaaac tgcagatgag taatgatacg ctgataattc
    29581 aaagattctt catcgaacac gatgccgaag aatttactga tgatactcag tatatctctt
    29641 accattgtgg tagcggtcat cgtcacttgc atgtcttcgt ttccttgttg gctattcaca
    29701 aaatgaaagg caataaaacc ggcttcatcc tcagaaagtt ccacacccgt cttttgtttc
    29761 acgaacaaaa gagcctcttt cgctgcttga tattctttcg gataaaattt ttttgtttca
    29821 aataataatg gattaggcag ttggatattt tcttttgctc gtgtgatcgc ataatgaaga
    29881 tgatcggtca atgtcagata aatattaggc gaaaattcaa cttttagttt atcttcagcc
    29941 agttcgacta cttgtttgac ccattcgatc tcctctgccg gaatatcagc catcagctgc
    30001 tggaattggt ttttagcaaa agaatctctt aaaacaaatt ctttttggac cgcttctggc
    30061 gaaatctcgt ccccaatctt tttttgaaaa gctagtcctt ttcccataca aacgacttct
    30121 tccccgaatc catttttcga aatgacgaca ttgttattca aaattttatc aattttcatt
    30181 ttgcttcgcc ctctcaagaa aaataaaaaa acctagaatt cgcctactgt ttcttagctg
    30241 aattctggtt ttgcctgccg tacagtaaca atccatttct tattcaaaat catcatacca
    30301 tcgaattttt atttatgcaa gccttttcat ataaaaaata aaaaaactgg aacacaagtc
    30361 tatctagaaa acaaccaaat cagagatgca cctctttgcg tttctctttt tttcagcaaa
    30421 ttggtttttc cttatcgatt catatccttt ggctgttttt aatctgtaac gacttatgtc
    30481 ccagtatcct aaaatgtttt tgtctctaga ttgaaagcag atctcatatc atctgtcgca
    30541 tcttttatcg atgtaacact ttccagttct ccttgtacaa ctagtcgcgt gacggcgtcc
    30601 atgtcgccac aagtaattag tgtgacaatt tcttttccct cgacttcatt cagtaattgg
    30661 gtatctgtag gagcaacttt ttctttaaaa aatgttttgt atgtatacac attttctaaa
    30721 tctgtcaaat agatcttggc acccatctct aaattttcta gcggtgtaaa cagcaattca
    30781 gggttcgttg cacgatggct tgctaaggca taatttcctt ttcccatttc ttgatctgga
    30841 gataatgttc ctgctccgta aagtagtgac gtattatcca agcctttgaa aaccggtaaa
    30901 ttgattgaaa ctgaagggac tgcgatacta gcaatcactg gtagatctgt tcctctcatt
    30961 tgcgacctca tgaccccttc tgtcgtcatt ggttctaccg catcaaagtc aaaactgaca
    31021 tctttatcgt tattcttttt cagatcttct tttgttacat tcgctatagc atatttatct
    31081 cctgtctctc tgacaaaata atccttgatc tgttcattga aaatcaaagc tagtcctact
    31141 aataacaaga ggaacaagag gatattgact agccatcttt ttgcttttcc tttttttttc
    31201 attttctctc cctccagact cattttactg tttttctttt gctctttcca acagccgaat
    31261 gacacatttc tacaactctg tgtatttttt tcgtacacct gcgctttttt ctacaggata
    31321 tttcaaatta tttttgatca atttttcttc aatgatctca tctagatcca ggtgaaggtt
    31381 gtctgccatc atgtatgaat agatcaatac atctgccagt tcttctttga ttcgttctaa
    31441 ttgtgtttct tttacttctt ccggcgtttt ccattgaaaa agttctaaca attcagatgc
    31501 ttccaaagaa atagagatag ccaaatcttt ttcgttatga aattgtcgcc aattacgttc
    31561 atctctaaac tggttgactt tttccatact attcatttgc ttgccttctt ccaatcactt
    31621 cttacttgat taatcatacc atttttttac ataaaaaata gatgatgctt ttaaaaaaac
    31681 tcctgttttc ttttatttaa aataaatgtc atagaaaatc aataatttag gtagccacca
    31741 tgtgtatgca gtagcgtgtc ttcttccctt gaggcttttt gtttttcgat acgttcttct
    31801 tcccaaacag acaacgctac tagaatctct cttaatgatt ttccttcttc ggtgagcgag
    31861 tactctactt taggcgggac ttggttatag gcttttcgat ggatactttc atcttgttct
    31921 aactctctta actgctgggt cagcattttt tttgtgatcg tagggatttg cttgcttctc
    31981 cacgcctaaa ataagcgata ttgctcttcc ctttatctgg tgtaagcaaa gcagttagca
    32041 ttttgacgat cgtgggttcc gcttttcact tcttatctac ttagtatcat gaagtatact
    32101 ttcgtactaa tactaagtag cggaagtgtc aactactttg tttaactaca tagcaaaaga
    32161 aacagaaggg tgacaacggt catcacgctt ctgtttcttt tgaatatgct gtgttgttgt
    32221 ctctatctcc gtgtagactt tcctcaaact tatcctttga cagtaagtca gtaggctgat
    32281 ttgctctttc tagatgccag atacatttct acaaaaaaat agtgattctg ttttgaaaat
    32341 tcgattttta gttctgcatt ttcgtaaaca tccaacattt ttttcgcact gtatagacct
    32401 aacccgccat tttttttaga ggtttttcct cttttgacca tctccgatag cgtcaaattc
    32461 gttggatttg ccttgttcgc tatactaaaa cgtgatccct ctttttcttc cagcagattg
    32521 atcgtaatcg gactctgatc ggctacacga tgatcgaatg cgttagaaat aaaaatagat
    32581 aaacagcgaa taaaaacaac cagatcaatc gggattgttt tcacttcgac aggaataact
    32641 acttggatcg ggattttctc cgattctgct ttttctagaa attttaaaaa gacacccctg
    32701 acagctgggg atacaatatt tgttagctgt tgtatttgcg gtttttcaaa aatagctgca
    32761 gaatgatctt ctaatatttc taaaaattcc aacgtttctt ttttattttc ctgaagagta
    32821 atttttaatg aaaacagtag acttttataa tcatgtttga attcctctag atgatggtaa
    32881 tattcttcat ttatttgata cagttgaagc gattcctgat gttgtctttc ttttacttgg
    32941 aaactgatca acataatgga aaaaaacaaa gaaaaaaaac tgatgactat cgacaaaaga
    33001 gatagaagca gataattaga tgagcggaag tgaacacttc ccaataccaa gtaaatcacg
    33061 ccccagctta ataaaataca aatacctcca aaatagtgtt cagttggata agtaaaaagc
    33121 aagttgagaa atctcgtctg tttgtctata ctttttacta atagatagaa aataaatgcg
    33181 ataagtagtg caaaaaaact tgctgttcca agatgtctct ttcctaaata atccatcaga
    33241 tcaaaaacca gaaaaaagct gagtccagtg atagcaaaaa aaagattact gatcaaacag
    33301 ctgataaaca ggtttttcgt agtaaaataa aaaagaaaca tcaataataa gccaactagg
    33361 taaggaaagc tcactttagg gataggaagt aaaagtgtca ccaccatcaa agctatacaa
    33421 ccaaagctgt tttttactag cagttgtttc gaaaagaata aatagatacc taaatctaaa
    33481 atcaaagtaa aggtcaaata aagtgtcact gttgcgacca tcttattcct ccattgtttt
    33541 gtttcaaaat gtacacatat ttttttaata ggagcctgca tatgaaaaag tattttatca
    33601 tagaagataa ccagtttcac agcgaatgga tcatcagaga attggtcaat catcatatcg
    33661 agaatatcgt gcagctcact tctatctctc aaatcaatca gttgatccag ttgggcacta
    33721 ttgataatca cgatgttttc tttatcgata tcgacttgaa gcaatcactc acaggtattg
    33781 atgtcgccga acaaatacgt aaaaaaaatg cacatagtaa tattgttttt ttcaccgctt
    33841 atacgaattt tgcagtagag atcatcaatc ggaaaatcct tccgattgga tatatcagaa
    33901 aagacaggaa tttatctgaa caaattcgtc aagtgctaga acagatcaat tggaaagaac
    33961 agcagatcgt aaatgtcgat aaaatcttta ttgatcaaaa aggtggaagt ctcgtcctgt
    34021 atcctcggga aattttatat attgaaagta tcaaaggttt gaaaaaccaa gttatggtca
    34081 aaacaactac agaagataaa ttagtgaata cttctttaaa acaattgaag acagagttag
    34141 aaaagtatgc ttacttttta cttttaaaat cttactgtat caacttatcg caattgcaga
    34201 aaattgattc tgctcattat gtgttagaat ttttcaacgg tgactcctta ctcgttggtc
    34261 gaaagatctt tgaaaagaca aaggaacgtt tccatgactt ccaaaaaact gcttcgagtt
    34321 gatgaataag agctgagacg taagcatagc agatttcagg gagtgggcct tgaatcggct
    34381 ccactccctg aaatcgttga tatgatttta cgcttcctct tctgtaggag catctgttaa
    34441 cgaccatgtg aatgttgaaa cgtagttgtc tacagcataa ttggatacag gaacaaataa
    34501 ttccactgat tgtctccctt gttccacatt ctctcctaat gaataaatcc atgtgccaat
    34561 cccttcattt gctcctgcag ttactagatc tactgcctga tctgctggga tttcgattcc
    34621 ttcagcgcca gttccagaaa cggtagatgg cattccactg ctattagttg cgctatttcg
    34681 aatcacggcg tttttgaatt tcacctttga accagttagt ttattcgtat cagatgttgt
    34741 accaataaat tctgttcgtt tgactgataa tttccaaccg gcataatttc cgcttttatc
    34801 cgttacttgt acgaaattct gtacttgtgt ttgtgctcca gtactgtccg ttccgctttg
    34861 taatgcagca tatactgttt ttgctgtacc taacttgatt gttccaaaat caagatctgg
    34921 agcttggtca atcgtcagta atccggatgt tccctgattt ggttcgtcag gtgtaaaagg
    34981 agtatcagga tcatttggat caacaggagg aactggaaca acgtcttcag gatttgcagc
    35041 atctggtttg gtcacagaaa tcgttccttc tgtagtggct gagctcttat tctcagcaaa
    35101 aacactttgt gtacataata atagacttcc agctagaacg gtacttaatg caattgtttt
    35161 tttcatttct ttttccttct ttcttttaaa ataaataatc cagcaaataa gccaataaga
    35221 atgactccaa caagtatcag ccatcgccag tcaaatgttt ctggctgatt ttttaaatca
    35281 atatcctttt cattcaattg gtccgctgca tcatcagtta tttcaaaatc ggtatggaga
    35341 ttccagcgtt tttctttcca agcacccttc acaaccgctt gataatgtcc cggagctaat
    35401 ttttttcctt ctaatgggac aagtaaatca aaatttgtat tgggtgctac atctattttt
    35461 tcttttttta ttgaaaggta aggctctttt gtttcttttt tgtagatttc aaccgatgta
    35521 gtcaagccga acatataaga agcatttgga ttttgaagat tcgccttaat cacatttctt
    35581 ccatcgcttt gatccgcata agcttttttt aatttcaaat ctggggaaga ctctttttcg
    35641 ctatttcaaa tcaagattgc tttcacttga gaaaactgat tgacgacacc aactgtactt
    35701 ttgttagaaa cgttctcttt tttttcttga aacgtgatcc ctcctgcgac cacaccagcc
    35761 agctcttttt ctggcgcttt cagtgaaagt gtctcagtga ccgcactttg aggaggaaca
    35821 gttatttctc ccactgtttt tgctaagttt ttcatatcat aacttaatgt ttcatcaaat
    35881 ttactttttg attctccata ttcgatcaca ccgtttgaat tcgtcgtagc ggagtggatc
    35941 aatgtctcaa ctgttactga tttatctgtt cggttaaaga ccgtgtaggg gatttcctgt
    36001 gttttcccag gctccatcct tacatttata tatgttttgt tcttatcaat ctgattagcc
    36061 ggtaattcaa aatctaccgc aaaattgaat tcggaggcat ggacaggtgt ctgccaaagg
    36121 atgaatccaa ggagaaggat cataagtaaa gagcagttgt ttcgatgact tttattccat
    36181 attcgattca tcaatcatcc tccttgatct ttattctgtc ggcgcatctg ctaatgtcca
    36241 tgttatctct gctgagtaat taccagctat ctgattttct ttggttaaga caagtttaaa
    36301 cgcccgaccg ttatttaaat attctgaata atctgcttgc acattcgttg aattttgtcc
    36361 tctgacaatt tccagttctt cttcattcac taattgatac tcctctcctt tcttgaaata
    36421 taggacatcc tgcatagaaa atcctgcatc atttgtgaaa ggctgagata attttatcgt
    36481 taagatccac ggatttttcg tttctcgctg atcataaatc cataattttt cctgtatatc
    36541 catttggcca aatgcttctt gtgtcaaacc agttgctttg atctgaccaa aatcgagttt
    36601 ggaaggggca agaaatgaga gaccaccttc tactacgatc ttcgtagcag gtgcaaaagg
    36661 cggggcatca tgataaacaa ctggcgtatc acttggattg tgattcccaa tttcatcgtt
    36721 ggtacttggc ttgttctgca cacccttagc agtagcttct cctgcttggt cgtttaatac
    36781 aatttctaat acatctcctg caaaaagttg attttctggg atagaaagaa caaattcccc
    36841 tgtatcatca atagttgttt ctgacaaaac agcttgattt tttttcaatg aaaccaaggc
    36901 tcctgcttca cctgtacctg tgatcgtgcg agaagctgca ttgatcaatg tggtacttaa
    36961 attagcaggt tcaggaggaa tcacatcgac tgtcgttttg gcagagtcca gttctgtcac
    37021 ttcttggcca cctcttactt ttttagcagc aacaacttgc acttgatcct ccgtttttaa
    37081 tctctcatca ttgggtactt gaacttcgaa caagccgcct tgttcgcctt ctccccatgg
    37141 gttatatcct gccccttcat tttcatctgt ttgtccttgc gtgagagcag taatttctac
    37201 agatgatttc gtcgtatctg cgtaagtaat acgtaaagtc acttctactt cgcctgtcca
    37261 agcatctctt ggctttgcct cgacaccttc gggaacaacg acatgcccat agattttttt
    37321 atctgcattc gttggcactc ttaaggattc aatcatcggg agctgattat tggcactgat
    37381 tcgattataa tttttcattc cggcacctgt ctgagcaaat tcatttgtta aacctgcact
    37441 agggcttgat acgaaattgc ctaagtcggt cccattgatc gataagtctg tcatatcaga
    37501 aaaataatcc gggtcttttt caaagttatc gaatgttgcg cctgtttttc gccataaagc
    37561 gatttctgaa tgggcgatct ttaaagaaga atctccattt ccttggaata cccagccgcc
    37621 tatgccattg gtctcgcttc caatccgttt gttttggaaa tcaaaatagg taggagattc
    37681 taagtccaca ttcaacacat ccccactgat gatgccggtc ttatcttccc ctgttttccc
    37741 gaccatgact aaataagttt tttcacctgc cgtaaaattt aaatcaatat ctccagttgt
    37801 atctacagtt gctccggaat ctgcgcgtat atcgatattt gatttttctc cagtgactgt
    37861 aaaatcagct gaacctggac tttctagaaa accagaagcg tcataaaatt ggatcgcttg
    37921 atttccttga ttgagtctgt tcacgtaaga tttgccatca cctgtatttc ttaccctcaa
    37981 tgagcctcca ttggttactg tcagcttttg cgtaccattc tcaaaacgaa ctgcgctgga
    38041 acgcccactc tgcttatcga tgtcgataac tgatccggct ccttctacga tcaaagaaag
    38101 gccttttcct acaaaacgca tcccattgtt cccgggttga tcgtcatctc cagccatttt
    38161 gaccgagatt tgtccaccac ttgatacatg gatttgacta gaatctcctt gcaagcgaaa
    38221 tccgcccgta gttgctgtat tgatggtgat tttcccacca ttcgatacag tgactttcga
    38281 tcctgaacct ctcatttgga tgattccgcg attgttgtca ttggtaatat tgatctttgt
    38341 atttaccgaa aattcgcttc ccgaaccact gacttcaaac atgacattac caggtagcgt
    38401 atcgactgcc tctccttcat cgctaacgcc atctgcccga tcataatcaa gagagacctt
    38461 tgaaccgctt aagatatgga ttcctggcgc tgtttttcca ctaccagtct catctcctga
    38521 atctagaaaa ccgtagaatt tccctagata cggtccataa agttgactac cattgatcac
    38581 attcaattga tagcttctac aaaaataggc tttacccgga gcggcattca gtccttcctt
    38641 cactggttgg acatccaaca caccggaaac accatctaat gtgacagttg cacgaggagc
    38701 gtaaatcagt cctaatttgg acccatcata caaatcaagt gtcccggtaa attttatttc
    38761 tcccgtaaaa tgatatctgc ttccccaaaa atcggtcata taagcaatga taatggcatt
    38821 tccatcgata tttggtgtca atccacctgg atgtgcagct gttcctttaa aattctggaa
    38881 ctcgatcgac tgaggtatct cattgatata aatatggtgt gtgccgaaat tgtgagcgaa
    38941 tccaccaaaa tcaataacga tatctcgttt gatctctaat tccctgtcta aagtcaaagg
    39001 ctgaatcagt cggatcttag ttactgattc gtctgcgaga agctgcgcca gttcttctgt
    39061 tgtggcagct tcgctgtacc catccgctga aacagctctt gccagtgccg cttcactgct
    39121 atcagacgtt tgttcagcta cttctgaggt ctcacttgtc ttattttctt cttgcctttt
    39181 attagtagaa gcctcacttt gagattctgt agtttcagaa gtaatcgatt cttctgaaaa
    39241 agtagtcttg tcaaaacttt gattctctga ttgcgttgat tcgtttgttt tctctacatc
    39301 ggcaaaaacg gatgtcacca tcaagagagg gaagacaaac aaaatcgacg caatcagctt
    39361 ttttacacat tgattcattc tccaccatcc tctctgacgt tttgacaatt ttattttatc
    39421 gttttaatct atttccaatt gtactttcat atttaaaata tgtattttta tggaaaaatt
    39481 tttctattag gtgtaaattt cttttttgta caaaaaaaag gctgtgacaa aagtcttgtc
    39541 acagtccctt atcccgaaca agcggtgttc tcccgccaac tcctcggtca caacctccat
    39601 tttaacaata aacctgtaaa tgtgagggca atactttgat tgtcacaggt aatttatccc
    39661 cttcatctcc atcaacattc gtcgtcaatt cttcctcatc agctaatgac acaactaatt
    39721 gctcacaagt aaggtaggct agatttttcg tagattcatc tactcctttt aataggtctg
    39781 gcaatgtttt aagcgtatcg atcaaagaag aatcttttaa ataaaccaaa tggagcttac
    39841 catcatccac ttttgcattt ggaagtaatg tttcaaaacc gccgactgag ttagttagtc
    39901 ctatcagcaa ggtactgctc tcaatttctt ccttctttcc atcaacttct acgtgaaacg
    39961 agtaggattg tgtggaagct aactgtttga ttcctgacat gaaataagct agttttccaa
    40021 atttcgtctt cttctccgca tctacatcat tgattgattc agggatagag ccaattgcga
    40081 cgacattcat aaaatagtcg tcatttattt ttccaatatc caatggcttg actgattcaa
    40141 tcgaaaaatg attgatcgct tcctcaggtt ctagcggtat ccctaatgct cgagccaagt
    40201 cattcaccgt acccaatggg aaaaatccga aatttggccg atgttcttgt tcagcgattc
    40261 cactgattcc ttcgttgact gttccatctc cgcccatgac aaacacacta tgataccctt
    40321 ccgtagccgc ttcgcgagta aagttttttg catcccctgc tttttttgta tgcaagacga
    40381 ccacttcatc aaacaccgat tccaattttg ctatcgctaa ttgctcaaat tcctttgctt
    40441 gttcaccgcc tgaactgggg tttaccacta ataaaacttt tttcatcact ttctttccct
    40501 tctgattgat ttctttaata taacgatagc ataaagaaaa aaattaggct gtgatgaagt
    40561 actgttcatg aaacattcat ttattttttg acaagataaa acatccatac tatgaaactg
    40621 aggaaaatta aaacaatgat ataggaaata aaagaaaatg ataggcattg tatacctaat
    40681 aaagtaaaaa tcttaggtgg cagataaaaa cacaaaaata gcaagagaat gtaatagcgt
    40741 ttcttccact ttttgttatc agaccttata gcgatcagag gaataccaaa tgcataactg
    40801 aaaaactctt gatgtactgt taatatgtca cggccttgaa taataaatgc gacaatcccc
    40861 gctataatta gtgtgcctaa caccaaaacg gaaaattgat gaatatattt tatatacaaa
    40921 tgatccattg tacttcctgc ctttttattt gtgagaaaaa caaatccgta tacgtgaata
    40981 acttctagaa ataaaagaaa tttgcaaaac cactccattt atctacttct acgtgcattt
    41041 tttgatattg ttttctttat ttaagattat acagaaaaat atctagggtc gctggattct
    41101 aagtaaaaaa aatttttaaa ataaaaagaa ctaaatcaag tagtaattaa actactgttg
    41161 atttagttct aataaaaaga tttagatgaa cagttttatt gatttaaata gttcatattt
    41221 tttatttata ccgcttattt actacttgct gtactagaat ctgaagtgaa tgtactcaaa
    41281 acattttcaa agtctttgtc tttgattttt acattagcgt ctttcaatac ttcgccaatt
    41341 actttattct ggaaggtact gtcacttaat tttgtgtctg ttgcaatttc tttcaattct
    41401 tttttgtatt tatccatgtc gttgcctttg ttttggtttt tcaccatttt aaccacataa
    41461 tattccgtag tataagttga agcattggta gaagtgatca catcagaaac ttgaccatct
    41521 ttcaacttaa aggctgcttc tttgacttct gccggaaccg ttgtagtagt ggaatcaaat
    41581 ttcacttttc cgccatcttc ttttgtcgta gtatcagttg acttgtcttt ggctaattta
    41641 gagaaatcgt ctcccttatc agcagctttc ttcacatctt ttgcatcatc ttcacttgct
    41701 acttggataa tttgagcttc cacttctgga tggaaagatt cccaagccgt ttttaaatct
    41761 tcatcggtca atttgatatg tttctttaga ccttcttgaa aggctagact ttgtttgata
    41821 ctgtctttga atgtttgttc tgtgtaacct gcagatttca attgtgaatc aaatgaatca
    41881 cccaattgtt tctttgtttg atcatattga gaatcgattt ctttggttga tacgtcatct
    41941 ccatatttat cttcaaatac ttgataaacg atcaactgtt gcacagcgga atctgctcct
    42001 tgcatttttt tcaagctatc cattacttct tgttcgctga ttgtaccgcc tttataacta
    42061 acggctgttt tttcaccggc attcgtacag cctgacaata agccagtgcc tagtacgatg
    42121 gcaagtgcta aaaatctttt tttcatttat caattctcct acttttcgtt ttttgcgaat
    42181 tgctaaaaac aaaggtgttc aagatgaaaa tcatcaagaa cacctttact aatctgctcg
    42241 atacgtcctg aatagatata aggctacaca atccagaaag ttcgctcagc agaataaatg
    42301 tcaagtagca catcacacct taatatacta cttcttttgt taccgttttt caagtctatc
    42361 ttgcgttccg tatttaacgt aactaattct tagaacaaat gttatcgatt ttgtttttct
    42421 tcttccgctg ctgggatatt caaatcagac acttttatgt tcgattttcc agacattttt
    42481 ttgttgtttt tattatgata tatttttata caactaaaaa tgaatatatt aaaactacca
    42541 aaaataactt ttaacgctgt ttcattatat gttttttcta ttttatataa acttttggat
    42601 acttaatacc aaccggttgg attgtaccaa catgagaatg tgtcagttct atttcctgtc
    42661 gcacttgcat aggcccatac tccttttgtt gcatttgctg atcgaataga gtttccaact
    42721 ttcgcttttg caccgtgacg attatagaca cgactatagt ggttagaatg acctgttttt
    42781 ctcgtacaag ttgtatcact ataataaacc catcttccag agctatgatt aattgctgtt
    42841 ctagaaattt cccggtctac tggtaaattt gacttaataa tattaccttt agcatcaaaa
    42901 tgaatctctc ctggctttaa agatttgtaa tagatttcac cgtgaactga atttgcttcc
    42961 acttcagatg acatagcgtt tcctccaaca aaacctatcc ctagtgcaca aataataact
    43021 gcttttttca taaaacgaca tcctttcctt tttgattttt aaagcatgat cttcttgttt
    43081 cctattttgg tcagatatgg atcatgcgtg actacgataa ctaacgtacc ttgggaagct
    43141 tgttgcgata agatttcaac tactttatcc ttatttcttt tatccaatga cccagtcggt
    43201 tcatcggcta aaatgatttt gctattcttc aataagactc tcgcacaggc gattctttgc
    43261 ttctcaccac cagataatgt gtagataggc gtacttagct tcttgtttat atttactttt
    43321 ttcaatgcct catactttgc tgttcgctta tctgaaacat ttaaattcga aaacttcaat
    43381 ccaatatcta aattaaaatc gattgtttca tattcaatca acccaaaatc ttgaaaaaga
    43441 aaagatatgg tatttctttt tatcaagcgc ttctctctcc cagttaattg tttcgtctct
    43501 tgatcgaaca aatagaattg tcctgaactg aactgatcat tcaatcctaa tatattcaat
    43561 aacgtacttt tacctgctcc actttctcca acaacagtca caagatcccc agcatccgca
    43621 gtgaaacgat ccaaatgaag aatcgttttg tttcgaatga tttttttcgc accttttatc
    43681 actatcaacg gctccaattg ttaacctcca ttcatcagca ataactgact tttcttttct
    43741 gtagacaaaa taaaaacaac tgaaagaata aaatcaatga agctaaagaa aaaaatatta
    43801 aaaagatatt taggctctct ttctataaga aaaaggaaga ttacacttgt cgttccccat
    43861 ttgatgaggt tcatcatgaa gaaatcttta tacgtttcaa agagcgaata accacttgaa
    43921 cgaaagatag cgattttccg acaattgtag acaaaatata tgcataacat gacatagctc
    43981 aacaagaaga aagaaaagaa agtgagtaaa aatatctgta tcaagtaatg aaatgatctg
    44041 cttaacactt tgacagctat ctcttcagtc tgagtatacg gaaatgataa ctgcaagtta
    44101 tcacgtaact gactttcttc aatcagctca aataaccttt ctttcgacaa agagcctatc
    44161 ttgatcttta acggtggata gatttctccg ctgagaatat ttctttccaa atacatactg
    44221 tttttcaatg tcagtatttc aacaaccgga taatgttcga tccaggggat agaaggaata
    44281 aatgtataaa tgggttgatt gttttttata aatagaaatt tcggttcttt ttcactgatt
    44341 tcttgataat attctgttat ttgcgccagg tcatttttta aatctgtatg tctatatcgt
    44401 tcagggatca atataacagg gtgcgtttct ttttcggtta tttggatcaa ccttccagag
    44461 acatcaatca aaggaaattt cttaagataa ttcagattta tttttatccc tctgccaaac
    44521 tgttcatttt cttcattcaa gtaatctgat acattgacta atatacttcc gtttttattc
    44581 aaagtttcat ataattcctg atctgctgct gatgcaaaac gattggaata aataaaatcc
    44641 acgtggtttt tcccgatggt tactggataa aatacatgat agtcctcacc agcgatttct
    44701 tttggtggtt catgggaaac agctaactgt gaagaagaaa aaacgatagt tgctaattcg
    44761 acattggcaa cggtcaaaag aagagaaaac aacaatccaa atgtaaaaag aatgtattgg
    44821 atatagttga acgcttctct taacgaagcg attttttcaa ctaataaaag agaaatcaaa
    44881 gagataccat atattccgct gaacaagaaa acgtacatct ggaacaaata aagaaattgc
    44941 tccttataat acataaaaat aaaaccacca atgattcctg caattatgaa tgtaataatg
    45001 attttctttt tccccaataa acaccacaat acttgatgta atgaaaagtc attattcaac
    45061 aataaactga tttctttatt catcaataag aaaaaggatg ataacaaaag gaataaaaga
    45121 atcaatccaa tagttaaata cactcgataa ttatatacgt ctgtatttga aataaccgaa
    45181 tacgaatagc tattttcaaa gtcttcaaaa atattagtgg cataatcagt gtgaaatgct
    45241 tgattgaatc gatcagtgaa tagtgaaatg aaccgttcat aggcttcttt ttctctcgtc
    45301 atcaagaaaa attcaccttc aaaatctttt attttgatcg attcttcaat ggacgttacg
    45361 cgaaaggagg tattagaata gggtaccttc tcgtgagaag atttgacatc cccttgataa
    45421 tacaatgtca tttcaagctt tggctgtatc ttgaagttcc aagatgtatc tacttcattg
    45481 gattcattta ctgttttttt catataagaa agctcagttt ctgatgagac attttcaaat
    45541 accatgatta gctgctcata ttcttcttgc ccaataaaat gttgtggcaa tcgaatcgga
    45601 gtataagtat ctgtttgatg gaaaaattca tttgtccgat agagtacctg tttatttata
    45661 ctaaccaagg caaaaccact catgataccg gtcactatga ttgtgagtaa aactgtgaac
    45721 ttttttttga ttatcaccac tctcccttac ttaaatgatt ctttgataaa atcataccgt
    45781 ttttcatcaa aaaaacgtat ctatcaagtg tataggtcat gtagaataaa tctatatact
    45841 gtagaatcga aaggagaaga agatgaacac cggagaaaaa atacgaactt accgcagaaa
    45901 ttgtaagctt acgcaaaaag aattaggcga aaaaattggt gtttccgata aaacgatttc
    45961 tagttgggaa aattctcgta caatgccaga ccttgaaatg ctgtcacttt tacatcatac
    46021 tttaggtctg ccgattgatt ttccttctat tgaatcagct accgaatcaa tagaaccaac
    46081 attgccagat gaacctcttg ttaacaagaa atcttattca tcaaaacaac ccttctcact
    46141 caaaaagaca ctatctttct ttctgctcgt cttttgtttg gtctatcttt ttcttatcaa
    46201 ttatcaatac aaaaatgaaa cgatcgatcg attcaatcct tttttgaaac cacaaatcgg
    46261 ctataccaca ctccctgtag gatacgtgga caattccatc gaacgttggg tatgtaatga
    46321 tatctttggc catggtacat ggctgtcttt tcacgctgga gaggtagaaa aagagaaaaa
    46381 ccatgctgta atcaaacaca aaggaaaaca cgtcatgaac gttcgcctgc tcacttggga
    46441 agccattccc tcagattacc aagaaatcct cagccaagac tatgacgacg ccttaaaaag
    46501 aagccctcag gagccagata ggcattaagt aggagatgaa cgtagagata gaaaaaatca
    46561 atgcacatga tcttatccaa aaaacagggg tacaaattaa ttgaaaaagc cttgagatga
    46621 aagttcctaa ataaaaaagt tcagaatgat aggtatttct ggcttatagt ataatattta
    46681 cgccacagcc tcccaatgat tctttttgga atcataatca aggatggaca ttcacaaaaa
    46741 tattttgctc attcgatccg aatagattaa cgaccaatct agtaagtaat atagattggt
    46801 acgtagtcaa gctgcacata atttagatca attttagttc ttttttatat ttctatgatc
    46861 tgctcattta cttttatatg atggcttaca gtaacataaa taaatatgat aatcgctggt
    46921 agtttatcca agagtactca ataaaataca aaatggattg ttcaagcgcc tatcataatg
    46981 ataaaatgaa acaaataaac gatacaagga ggaagagact ttgagtaatg gcagaacaat
    47041 tttttcattg taaaaattgt tcagttgtta atctatggga tttcggtagc atgggcattt
    47101 ttttatagag gaatcacacc aacctggccg ttttatctct tgttagtggg aatattagta
    47161 ggaaattttg cccctcttca ctatcgtacg aacttttttc caactgattc tggccttttt
    47221 cttaaaacaa agacgcgaaa attagaaaac attattgaaa tgacaattac ctgtataatc
    47281 attttcttag ttatattcct aaacgtcttc taataagaat ctcttaaaat tagcttgtta
    47341 cagttagtta cctagtttat ataacacagt tatcttatct tccttatcaa catcttttgc
    47401 caactctttg tcttaaattc gaaacatata agcagagcta cgaacaccaa gactgtttcg
    47461 gctctgtttt ttgcatcaaa ttcactctta tatagctgat aatctcggct ctttaaaaga
    47521 aattattttc gcttcatttt attataaaaa ggctaggaac cttgatgtta tcaggttcct
    47581 agcctttatt taagtattac cccacagacc cttccatata gatttttaaa ttacacacta
    47641 gtttaaacta aaacaaacaa ggaaaaactt accatttaat caaaaaatta gctcatatga
    47701 atatattaga atgtaaatta gactggtcaa aatctggtca aaaaagagga cgaatacagg
    47761 tagtcctctt ttttgttatt ttgatattct aaatcatttt ttattggatt attcccttgt
    47821 tcgatataga cccaaattga tctatcaata attatcaaac tgagtcatat agagacaaaa
    47881 atttcaaaaa tgacctgtta aatggtcatc gaatatcatt taaaatcaat acgacggagt
    47941 cagacacgga gtcaaaacaa gcccgctgag agtaaaaaat atacagtccc ataggtaaag
    48001 tgccacagga ctgtatattt ttttatttga gattacctga aatgattcca tttaattttt
    48061 atctcatttt ttcctactag ttagtatcaa atactctcct attttctcaa taaactattt
    48121 tgggttacat ggtatctgaa acagctaaaa ccttgtcatt atagcaaaaa atcactttat
    48181 ctccgtttca tttcagatac atttcactta agcggattcc aaaaaaacaa aagaaaaagg
    48241 aacccctagt tagcctatgc tttttattgg ttcctctcaa aacacggtca tataacaacg
    48301 gggtggggta aatgaatgaa aaaggtggag gaaacattat tcaatagctg tcttaaatac
    48361 ttcgttataa aatgtgaaca aataaaagac atctatccct attttctaca cttccacttg
    48421 actaccatct ttgaatcgta caattttctt aactcttctc gtttacctca attcattctc
    48481 aagtggaatc aactcatcat ctaacatttt acgccatgct cgcttcattt cttgtgggta
    48541 tttaaatggc ttcgcttctg gatgtttggc tttatatcct tcgtaatcct tactgttgat
    48601 aattgctttc atattaggaa ttggatcttc acctggtaga tattgcagat tagaagcatg
    48661 taattctttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat catcactcgc
    48721 ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttactatcta aagtccccac
    48781 atcaaatgca tttaaaattt gtcggtaaac ttctttgact ccttctgttt tattcaattt
    48841 agccaaagct ttttcaattt cttctcgtgc atctgaacta ttcgcagcat aagttcctag
    48901 taattgatca atataagttg aatcaatatc atacaactta gtggtatttt cactataaaa
    48961 ttcgatcgtt gaaaaatctt ctccttcttt ttctggttct tcctctatta atgaaccctt
    49021 cacggtctcg tacacgccaa tttgttcttc caaaagttga atttgattgt tcaaagttgg
    49081 agatttcacc atgtcatcat tgtaatcgtc ataagtcact aacgattcat aagcacgatt
    49141 caattcttga aatgctttga cgtactcgat acggatttca agtggcgtat gttcatcaat
    49201 ggattccatg gatgaaacct cttttaactt accatatgct tgtttgaatc tttttttgga
    49261 ttcctgataa cttggataaa tcaaacctga ttcttctttt gcttttgaat acagttttgt
    49321 cgcatcctcg acattcttct ccattgtcgc tggcttacga aacgtcacaa tcaatccttt
    49381 tgtcttatca ggatacgtac gattcgtacg tgaaaaagct tggatcaatc ctgcatactc
    49441 tagattacga tccacaaata gggtttgaat cgtaggggcg tcaaaacctg ttaatagacg
    49501 atccactaca atcgctaaat cgatttgttt cccaaattgt ttgaattccg ctctttttcg
    49561 tgccaaacga ttattgatgt ctccgttata acgctcaatg tctgctaatg accaggaagt
    49621 atcataatac tgattatatt cctcaataat tttttgcatt tcttcttgtt gggcacttga
    49681 atctcggcta ttttcttcta atgaataggt aatagcgatt cgtggaaaat caggatcctc
    49741 catcgttcgt ccttcacgaa tgagctgatt gggaaactct tttgttaacc aatcaggctc
    49801 tttcgtcatc tctttgatgg cccgataata ctttttggcc atgtcaatgg aactcgtggt
    49861 taagattgca gatttcgttg gcctgccatt tcgaaaatca aattttgtat aggcattatc
    49921 tggacggaac attttatgaa tgactttttg gatatgttca tctgcttcaa aaacagctgg
    49981 gtccaaataa ctttcttttt tgacaggttc catttgatca atcatacgat tgatctgttc
    50041 tgatgaatac tctgcatatg tttctacttc ttttaatttc cgatagattt tattctctag
    50101 tgaagtcggc tccacggtgt tttcatgctc tacttgaaat cctaagacag agccatcttc
    50161 tagcgcattc ttaatggtat aggtatgcaa gacctctcca tactgatcat gggttgtacg
    50221 tgccaattgc cctttggctt gtttccgatt ctcaggaaaa atcggtgtac ctgtaaagcc
    50281 aaaccaagtg gatttcggaa acattttttt gatttcttcc atattttcag cact
//
LOCUS       DASJPC010000007        50242 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJPC010000007 DASJPC010000000
VERSION     DASJPC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.007"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            374..1297
                     /locus_tag="U4A13_000418"
     CDS             374..1297
                     /locus_tag="U4A13_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778746.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            1506..2084
                     /gene="pgsA"
                     /locus_tag="U4A13_000419"
     CDS             1506..2084
                     /gene="pgsA"
                     /locus_tag="U4A13_000419"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN1778747.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            2324..3565
                     /locus_tag="U4A13_000420"
     CDS             2324..3565
                     /locus_tag="U4A13_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN1778748.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            3674..4723
                     /gene="recA"
                     /locus_tag="U4A13_000421"
     CDS             3674..4723
                     /gene="recA"
                     /locus_tag="U4A13_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN1778749.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            5134..6690
                     /gene="rny"
                     /locus_tag="U4A13_000422"
     CDS             5134..6690
                     /gene="rny"
                     /locus_tag="U4A13_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN1778750.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6769..7575)
                     /gene="proC"
                     /locus_tag="U4A13_000423"
     CDS             complement(6769..7575)
                     /gene="proC"
                     /locus_tag="U4A13_000423"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN1778751.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            7716..10289
                     /gene="mprF"
                     /locus_tag="U4A13_000424"
     CDS             7716..10289
                     /gene="mprF"
                     /locus_tag="U4A13_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1778752.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            10431..11294
                     /locus_tag="U4A13_000425"
     CDS             10431..11294
                     /locus_tag="U4A13_000425"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN1778753.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            11539..12477
                     /locus_tag="U4A13_000426"
     CDS             11539..12477
                     /locus_tag="U4A13_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN1778754.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            12500..13180
                     /locus_tag="U4A13_000427"
     CDS             12500..13180
                     /locus_tag="U4A13_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1778755.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            13153..13977
                     /locus_tag="U4A13_000428"
     CDS             13153..13977
                     /locus_tag="U4A13_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1778756.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            14144..14962
                     /gene="purR"
                     /locus_tag="U4A13_000429"
     CDS             14144..14962
                     /gene="purR"
                     /locus_tag="U4A13_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN1778757.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            15465..16838
                     /gene="glmU"
                     /locus_tag="U4A13_000430"
     CDS             15465..16838
                     /gene="glmU"
                     /locus_tag="U4A13_000430"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN1778758.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            16932..17903
                     /locus_tag="U4A13_000431"
     CDS             16932..17903
                     /locus_tag="U4A13_000431"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1778759.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      17909..18011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18077..18646
                     /gene="thiT"
                     /locus_tag="U4A13_000432"
     CDS             18077..18646
                     /gene="thiT"
                     /locus_tag="U4A13_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN1778760.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(18727..19500)
                     /locus_tag="U4A13_000433"
     CDS             complement(18727..19500)
                     /locus_tag="U4A13_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778761.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            19767..21467
                     /locus_tag="U4A13_000434"
     CDS             19767..21467
                     /locus_tag="U4A13_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN1778762.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            21437..21655
                     /locus_tag="U4A13_000435"
     CDS             21437..21655
                     /locus_tag="U4A13_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN1778763.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            21675..22565
                     /locus_tag="U4A13_000436"
     CDS             21675..22565
                     /locus_tag="U4A13_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN1778764.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            22583..22867
                     /locus_tag="U4A13_000437"
     CDS             22583..22867
                     /locus_tag="U4A13_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778765.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            23354..23968
                     /gene="gmk"
                     /locus_tag="U4A13_000438"
     CDS             23354..23968
                     /gene="gmk"
                     /locus_tag="U4A13_000438"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN1778766.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            23973..24278
                     /locus_tag="U4A13_000439"
     CDS             23973..24278
                     /locus_tag="U4A13_000439"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1778767.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            24531..26939
                     /gene="priA"
                     /locus_tag="U4A13_000440"
     CDS             24531..26939
                     /gene="priA"
                     /locus_tag="U4A13_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN1778768.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            26954..27445
                     /gene="def"
                     /locus_tag="U4A13_000441"
     CDS             26954..27445
                     /gene="def"
                     /locus_tag="U4A13_000441"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1778769.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            27438..28376
                     /locus_tag="U4A13_000442"
     CDS             27438..28376
                     /locus_tag="U4A13_000442"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN1778770.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            28376..29734
                     /gene="rsmB"
                     /locus_tag="U4A13_000443"
     CDS             28376..29734
                     /gene="rsmB"
                     /locus_tag="U4A13_000443"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN1778771.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            29747..30487
                     /locus_tag="U4A13_000444"
     CDS             29747..30487
                     /locus_tag="U4A13_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN1778772.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            30484..32553
                     /gene="pknB"
                     /locus_tag="U4A13_000445"
     CDS             30484..32553
                     /gene="pknB"
                     /locus_tag="U4A13_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN1778773.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            32718..33617
                     /gene="rsgA"
                     /locus_tag="U4A13_000446"
     CDS             32718..33617
                     /gene="rsgA"
                     /locus_tag="U4A13_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN1778774.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            33630..34280
                     /locus_tag="U4A13_000447"
     CDS             33630..34280
                     /locus_tag="U4A13_000447"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN1778775.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            34281..34919
                     /locus_tag="U4A13_000448"
     CDS             34281..34919
                     /locus_tag="U4A13_000448"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN1778776.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            35074..35928
                     /gene="mreC"
                     /locus_tag="U4A13_000449"
     CDS             35074..35928
                     /gene="mreC"
                     /locus_tag="U4A13_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN1778777.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            35932..36441
                     /gene="mreD"
                     /locus_tag="U4A13_000450"
     CDS             35932..36441
                     /gene="mreD"
                     /locus_tag="U4A13_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN1778778.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            36696..38270
                     /locus_tag="U4A13_000451"
     CDS             36696..38270
                     /locus_tag="U4A13_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1778779.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(38338..40137)
                     /locus_tag="U4A13_000452"
     CDS             complement(38338..40137)
                     /locus_tag="U4A13_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN1778780.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            40260..40766
                     /locus_tag="U4A13_000453"
     CDS             40260..40766
                     /locus_tag="U4A13_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1778781.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            40845..41567
                     /locus_tag="U4A13_000454"
     CDS             40845..41567
                     /locus_tag="U4A13_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1778782.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(41645..43045)
                     /locus_tag="U4A13_000455"
     CDS             complement(41645..43045)
                     /locus_tag="U4A13_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN1778783.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(43215..44189)
                     /locus_tag="U4A13_000456"
     CDS             complement(43215..44189)
                     /locus_tag="U4A13_000456"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1778784.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            44542..45102
                     /locus_tag="U4A13_000457"
     CDS             44542..45102
                     /locus_tag="U4A13_000457"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN1778785.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            45119..48640
                     /gene="mfd"
                     /locus_tag="U4A13_000458"
     CDS             45119..48640
                     /gene="mfd"
                     /locus_tag="U4A13_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN1778786.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            48655..>50242
                     /locus_tag="U4A13_000459"
     CDS             48655..>50242
                     /locus_tag="U4A13_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1778787.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT        16180 a         9066 c        10908 g        14088 t
ORIGIN      
        1 tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttaagga
       61 agaggttgtg aaatcagcgt tttgacctga gtattagaga aacatacgga aaaatatctc
      121 cttatatttt gccggttttt gagctaatac cgaggaacag ctgattgaac accgtttaag
      181 aaagaggtcg tgagcctgcc gtttaactcc gagtcacaag gaacaagttc aaaaaacagc
      241 ttcttatatt tattggatat tgtgacttgt gaccgaggag ttggcaggag aacaatgttt
      301 attcggaaaa aaggagctgt gacatgactt ttgtcacagc acccaaacat aacgtctttc
      361 gttgcaacag cagatgcttc ggagaatcaa acagactgga gaagatgaac gagtggcttt
      421 tgaaaccatc ggagaaaaat tacggcaagc acgcctgaat aaaaaaatat ctttagatga
      481 attacagcag attacaaaaa ttcaaaaacg ttatttggaa gcaatcgaca atgatgattt
      541 tgaccaattg cctggtaaat tttacgtgcg ggcatttatt cggcaatacg ctgaagctgt
      601 aggagaagat ggagaccatt tagtcgatgt ttttgatggg aaaagcaaat tgacaggatc
      661 ggtccctgaa cgtccgcagc ctgaaacagt aagtacttct agaaaagcaa tgcatcagga
      721 agaatcgcat ccaagtcgct tttggacaag tcttcctgtc attttattag gattggttgc
      781 tttggcgatt gtcgttgtcg tgggctatat gacttggcaa gatcgtcaag ctgatccaat
      841 aatcggagac acagcttctt cgcttaaagt agaaggatca gtcgaggagt cctcttcctc
      901 agagcaaagc acgcagacca aagagtcttc tacagtagaa tcaacaacaa aatcaagtga
      961 agaaaagaaa atggcaatag ctacaggaga agattctggc agtgccatcg cagtaaatat
     1021 cacagacgct accaagcctg taacccttga atttactgct gtcaatcgtg tatggttagg
     1081 tgtgctagta gacaatgcct atgtttatca agggacactg gcagcaaatg aaacacagag
     1141 taccgtgtta ccggaaacag caacaaatgc gaccatcacg attggtgcag caagcaatgc
     1201 aacgatcaaa gcgaatggag aatccgtacc agttaatcca ggggaaaaca atcagagtcc
     1261 aaaaaatgtg aaccttactc ttcaatacgc agaatgatcg tataaattag tttacgcatc
     1321 tgtttacttg taggtataaa aaagaaggat cgtgacaaat ctgtgtcgcg gtccttcttc
     1381 gttaattcaa aaagatcaat ttcaataaaa atactccttt gaataaattc atttggaaaa
     1441 gtaaataata tcggtggaaa cttcgctgat agtcacgtag aatagggaag aaaggagtcg
     1501 atagtgtgaa tttaccaaat aaattaactg tacttagaat ttgtatgatc ccgattttca
     1561 ttttggttgt aggtttacct ttagattggg gacaatttaa cgtaggagac accacactgg
     1621 ctgtcacaca gctagtaggt gcagttattt ttgctttagc tagtatcaca gactggttag
     1681 atggaaaaat tgcccgtgcg cgaggccttg tcactaattt tggcaagttt gcggacccgt
     1741 tggcagataa aatgctagtt atgactgctt ttatcatgtt ggttgaccaa ggaaaggccc
     1801 ctggttgggt agtagcgatc attgtctgtc gtgaattagc cgtcacaggc ttgcgtttac
     1861 tacttgtcga acatggagaa gtcatggctg cagcttggcc gggaaaagtc aagacggcta
     1921 cacaaatggt tgctattatc ttgctgttta tcaataatat cccattcagc ctgatcggta
     1981 ttccagtaga tcaaatcatg ctttatgctt gtctgatctt tactatctat tcaggtgtgg
     2041 attacttcat caaaaacagc ggtgtgttca aaggatcaat gtaagtatcg tttagtggag
     2101 aggttgtgag cctgccgtct aactctgagt cacaagggat aatttcaaaa aacagctttt
     2161 catgttttat gtatattgtg acttatgacc gaggttttgg caggagaaca ctgtttattt
     2221 ggaacaagga gctgtgacaa gatttttgtc acagctcctt gccctctttt taaataaaaa
     2281 aatataaaaa tacagcgtat actatataag ggagcgggaa gtaatgaaag cagaaataat
     2341 agctgtcgga acagaacttt tactaggaca agtcgtgaat acgaatgcca ctttcttgtc
     2401 agaacagcta gctgatctag gtatcgaagt ttattatcaa acagttgtag gagataatca
     2461 gcagcgttta gaagagttga ttgctctagc tgaaacaaga agtgaactca tactcttatg
     2521 tggcggatta ggaccgacag aagatgacct gacaaaagaa gcaactgcgg ctcacctcgg
     2581 taagtctctc atacaaaata cggaaggata caaaaaatta ttggcatatt ttgaaacaac
     2641 ccatcgaaaa atgaccaaaa acaatctgca acagtcccag attatcgaag gcggggttcc
     2701 tctaccaaat cgtacagggc ttgctttagg gactttttat caaacagata cacatgcgta
     2761 tatccttctt cccggtccgc ctaatgagtt gaaaccaatg tttgtggaac aagtacgtcc
     2821 tttactagag gaacgttttc cttctgaaga aaaactaatt tctaaagttt tgcgctttta
     2881 tgggattggt gaatcgcgac tggtcactga gctaaaagat ctgattgaga cacagatcaa
     2941 tccaacgatc gcaccatatg ccaaaccaaa tgaagtgaca ttacgactga cagtgaaaac
     3001 aaatgatgtt caggcaggga atcaagctct tttagcatta gaagaaaaaa tccaagaacg
     3061 agtaggggaa tatttttatg gatacggcga tgataattcg ctagcaaaag tcgtagtgga
     3121 actactgaaa gaaaacaagc agactgtaac agcagcggaa agtttgacag ccggcgcctt
     3181 ccaagccgca ttaggcgaca ttgctggtgt atctgaagta tttccaggcg gattcgttac
     3241 ctactcgctc caaacaaaag ctggttttct ggagattgat ccggagctat tagcagaata
     3301 tggaacagtc agcaaagaat gtgtggagca gatggccatt caagcacgtg agatagcaag
     3361 aacagattac agtgtagctt tcaccggagt ggctggaccg gacacgttag aaggtcaatc
     3421 tgcaggaaca gtatggatcg ctatttcatc taaagacggc gtggtttcgg aaaaatatca
     3481 ctttgccaga gatcgtgctg ctatcagata taatgcggtt atgcaaggat ttgatctttt
     3541 gagaaaacag cttttaagaa aatgataaat gcgaatattt gttcggtttt ttcttgcttt
     3601 ttttcactaa aaaagatatg ataaggataa tgaaatgttg ctctgatttg taaatataag
     3661 gaggattatc gtattggcag atgatcgtaa agcagcctta gatgctgcac tgaaaaaaat
     3721 tgaaaagagc tatggtaaag gctctattat gaaattgggc gaaaaaatcg accaacaaat
     3781 ctctacaatt ccaagtggct ctcttgcgtt agatgtcgct ttaggtgtcg gcggttaccc
     3841 ccgtggacgt atcatcgaag tatatggacc tgaaagttca ggtaaaacaa cagttgcact
     3901 acacgctatt gcagaagtac aaaaaaatgg cggaacggcc gctttcattg atgctgagca
     3961 tgcgttagat ccgcaatatg cacaaaaatt aggtgtgaat atcgatgaac tacttctttc
     4021 acagcctgac acaggagaac aaggtctaga gatcgctgat gctttagtat caagtggggc
     4081 tgtagatatc gtagtagtcg attcagttgc tgctttagtt ccacgagcag aaatcgacgg
     4141 cgaaatgggt gactcacatg tcgggttaca agcacgtttg atgtctcaag cattgcgtaa
     4201 actctctggt tcgatcaaca aaacaaaaac aatcgctatt ttcatcaacc aaatccgtga
     4261 aaaagtaggc gtgatgtttg ggaatccaga aatcactcct ggaggacgcg cattgaaatt
     4321 ctacgcaacg atccgtttgg aagtacgtcg tgcagaacaa ttgaaacaag gtacagatat
     4381 tgttggtaac cgtacaaaga tcaaagtcgt aaaaaacaaa gtagcacctc ctttcaaaat
     4441 cgctgaagta gatgtcatgt atggtttagg gatctcacaa gaaggagaac ttctagacat
     4501 ggcagtagaa aaagacattg ttgataaaag cggtgcttgg tattcttata aagaagatcg
     4561 gattggtcaa ggacgggaaa acgctaaaat ctatatggct aatcatcctg aaatgatggc
     4621 agaagtttct gctctagtta gagcagctta tggtattggt gaagaagtcg ctgtcccaga
     4681 agatgaaaaa ggacaagaag aattgccttt agtagaagaa taaaaattat gataaacaaa
     4741 aacggctggt ctgaagagta ggtactctcc ttgacctgcc gtttttttat tgaataaaga
     4801 aatcgtgagt tatttaatta tacgagtggg tacataaaat aaaaatatct gttcatctac
     4861 aaagaataat ttaatagaaa agtttctcaa tttaaaggtt tacctatatg aaactagtgt
     4921 catttatttt aaaaaccatc gtttaatgaa taagtagaaa gccgtttctt tgtttattta
     4981 aagttgacac tacggggtac aaacattaga ataaagttgt gcatgttttt tgtacacata
     5041 tatgcgaaga tgcatattga cgagtttaga ataatttatc atagaagctg tatagatgat
     5101 aaaaatgaaa aagatagtat ggaggtggat tttatggtat tcaacattct cctcgctatc
     5161 atcggtttaa ttgtcggtct tggtttaggg tttatggtcg caaagtcacg tcacgagaaa
     5221 gaaatagctg gtgcacaatc atcagctgct ggaatcatcg atagtgcaaa aaaagaagca
     5281 gaaactctaa aaaaagaagc attgttagaa gctaaggaag aaaatcagaa ataccgttct
     5341 gaagttgaaa gtgagctgag agaaagtaag ttagaattaa aatctcaaga gaatcgtttg
     5401 attcaacgcg aacaaacgct gaatcgtaaa gatgactcgt tagaaaaacg cgagaactca
     5461 cttgaaagta aagaagaagc acttagttca aaacagcaat taattgaaga gcgagaaaaa
     5521 gacgttgaaa aaatgattga agatcaacaa aaagaactag aaaaaattgc tggtttatca
     5581 aaagaggacg cacgagatgt catcatgaaa tcgactgaag aagagctgaa tcatgagtta
     5641 actctaatgg taaaagaatc tgaacagcgt gccaaagaag aagcagatcg caaagcgaaa
     5701 aacttattat cattagcgat tcaacgctgc gcagcagatc aagtttcaga aacgactgtt
     5761 tcagtcgtta cccttccgaa tgatgaaatg aaaggtcgta tcattggacg agaaggtcgt
     5821 aatattcgaa cgcttgagac gctgactggt atcgatttaa tcatcgatga tacaccggaa
     5881 gcagttgttc tttccggatt cgatccaatc cgaagagaaa tcgcacggat gaccttggaa
     5941 aaactgatac aagatggacg aatccatcca gcgcgtatcg aagaaatggt cgagaaatct
     6001 cgtaaagaaa tggatgaacg tattcgcgaa tacggggaag aggcagcgtt tgaagtcggt
     6061 gctcatacac ttcatccaga tctaatcaag atcatcggac gtctgcgatt cagaactagt
     6121 tatggacaaa acgtcttgaa gcattcggtc gaggttgcaa aattagctgg tatcttagca
     6181 gcagagcttg gcgaagatat ccaattagca aaacgtgccg ggttgctcca tgatattggg
     6241 aaagcacttg accatgagat tgaaggatct cacgttgaga tcggtgcgga attagctgcc
     6301 aagtacaaag aaaacccagt agtaatcaac gcgatcgctt ctcatcatgg agatgtggaa
     6361 gctacttctg ttatctctgt ccttgtagct gcagcagatg cgctctcagc agcaagacct
     6421 ggtgcgcgaa gtgaatcatt agaaaactac atccgtcgtt taagaagtct ggaaaatatt
     6481 tcgaacagct tcgccggtgt agaatcaagt tacgcagttc aagctggacg cgaagttcgg
     6541 gtaatggtca agccagaaga aatctcggat ttagacgctg ttcgtttagt acgtgatatt
     6601 cgtaaacgaa tcgaagaaga actggattat cctggacaca tcaaagtaat cgttattcgg
     6661 gaaactcgtg caactgatta tgctaaataa taaaaaatac aaaagaagag ccgcggcaaa
     6721 agccacagct cttctttttt tgtaaggcac gaaaaaacat tgctatcatt attttttatt
     6781 caacatctca agatctttct cgatcgtcgt attgattcct ttgatgattg ctgggatgaa
     6841 tcctgcttct tctaaggcaa ctacacctgc aacagttgta ccgccaggag aagaaacttg
     6901 gtctacaagt tcccatggat ttttatcact tgaaaggaat ttttgacaag aacccataat
     6961 tgcttttgca gcaatctcag tggctgtctg tttggacaaa ccattcagta caccagtccg
     7021 tcccatcgca tcgatcagca tgtaaacgaa tgcgggtgag ctgccagcaa ggccaatgaa
     7081 tgtactgaaa tctgattctg ctaaaggata gacgctgccg attgcttcaa aaatcatcgt
     7141 agcttgctga agctgttcct ccgtggcagc ttcatttttg caaatcgcag aaacgcctgc
     7201 tcctacgctg acattgacat tcggcatcac acgtacgatg gaggcctgag gatcttcaag
     7261 cgctgtttct aaatctatta atgattttcc tgaagcaata gaaatgaata agactttctg
     7321 gctatattct cttagttctt ccaatacttt ttcaatgatt tttggtttca cggctaaaag
     7381 aacgatatct gattgtgatg caacttcctg attacttttg catgcatgga cgccagtttc
     7441 agcagcaaat ttttgtgcat tttcaaaatg tccactggaa acgaacacct cttctttcga
     7501 gactgttcct ttttttaata aaccatcaat aatagcttgt gccatattcc ctgtgccgat
     7561 aaatcctact ttcatgttat cctctccttt agcattagga tatctttctt tttctatcat
     7621 cgcaagcaaa gaacaggtct aaagtggaaa aattaaaata aataagataa cattggggta
     7681 tgacacaaaa gtatacaagt taggaatggg aaaatgtgaa aaataaacga tacttgatgc
     7741 tgcaatggat aaaggaacat agtctgattt taaaactgat ttttttcggc tccgtgttag
     7801 tctttgtggc aaatcaagtc gcaaatattg ctaatggcat gagttggcaa gatatttggc
     7861 aaaggatggg acaacaaagc cgcatgacga tcctgctcat gattctggct ggactgctgg
     7921 gtgtcactcc gatgttgttg tatgattggg tgacgatcag agtcttagaa aaacaaggaa
     7981 aaccaaaaat ggaaagaaaa gagtttcttc ttgctgcgtg ggtaacaaac acgatcaaca
     8041 atctcgccgg ttttggtggt gtggtaggag caagtctgcg ggctagtttt tatggaaagc
     8101 agacaaatag aaaaatggtt ttagccactg tatcaaaagt cgcactattt atgctgactg
     8161 gtttgtctgt atggtcgttt cttacattta ttgatgtctt ttttattcag tcagaaagta
     8221 tttttcgaag ctattggatc tggcttttcg gcggtagttt tttagcacca gtcattttct
     8281 tttttgctta tctaaagaga aagacattgt tcttcgagtt ttatccaaaa ggcttgcttg
     8341 gtctgctact ggcatccttt ggacaatgga cgggagccct tgcggtattt ttgatcatcg
     8401 gcaagttgat gagaatagat atttcgatgc tttcgatcta tcctatgttc atgattgcta
     8461 cattgatagg aatgctgacg atggtcccag gtggaatggg gacttttgac gttttgatga
     8521 tcttaggaat gagtcaattg tctgttcggc aagatgtagc agtagtttgg ctattatatt
     8581 atcggttgtt ttattatgtt ctacctttta tgacgggact tctattgttc atcacacata
     8641 caggagtcaa aatcaatcga tttttagata acattccgcg actgtttttc caaaaggttg
     8701 cgcatatcgt gctcgtaagt gctgtctatt ttgcggggat catgatggta cttctatcaa
     8761 caatcactaa tctgtctgca atcagtcggc tatttcaagt attattgccg ttctcattca
     8821 attttttaga tcagacagtg aatctattgg ttggcttttt attactcggc cttgctagag
     8881 cactttcagt aaaagtcaaa aaggcatttc tgcctacgat tgctttacta tcttttggta
     8941 ttttaaatac aatcacacgt acgttatctt ggcagttgat attggtatat gtcttgattc
     9001 tgttagctgt gtggttatcc agaaaagaat tttatcgaac aaaattcgtt tattcttggg
     9061 gagctataat ctttgatgct agcttgttcg gctttttgtt tatcgtttac gcaattgcag
     9121 gctatcacag cggacgcttt ttagtcggtc gaataacagg aaatcatttt attttgtttc
     9181 cttcagatga tgtgtggttc tcagggctta ttggtttggg catctcattg ctagctttag
     9241 tggcgcttta tcaatattta gctgaagata ctacgaagct tggcagtgag tacgaaaaag
     9301 accgttttga ccgtttattg gaaacatacg gaggcacagc tacaagccat cagcttcgct
     9361 tgtcaggtta cagttatttt tattaccaag aagacgacca agatcaagtc gtttttggtt
     9421 atcagatcaa aggaaacaaa tgctttgttt taggagatcc agttggcaat cctaaaatga
     9481 tgcgggcagc aacgaatcag tttatgaaac aagcagattg tttaggctat cagcttgcgt
     9541 tttataagat atcagaaagc tacgtagtga tgcttcatga attgggtttt cacttcacga
     9601 aagtaggtga agcagggatt gttgatctag caacagaaga cttgtctcgg ctgagtcagc
     9661 aacttgaata tcaacgtttg atgaaagaag gataccattt tacttggtac gatcaactgc
     9721 cggaagaatt acatcaagct gtaaatgaag tttcacaaaa atggcttcaa ggtgcacgag
     9781 aaaaatactt ctctgtggga cgatttgata aatcatatgt attatctagc ggtgtcggta
     9841 ttgtccgtaa agatcaaaaa gtcgttggct tcatcaccgc tcagccgatg acgaagaagc
     9901 aggcgggata tgatttgttg cgggtcttac ctaaagaatc tgaggaatta gctgactatc
     9961 tgttagccaa tctgtttatt gtatacgaga aaatgggcta ctcggaagcc aatctaggct
    10021 tagctccatt agctaatgtt ggagaaacag atttttcttt ctttcaagaa aaagtcatgc
    10081 atatcgtata taattatggt aattttttct attccttcca gtctgcttat gaaaagaaat
    10141 taaaatatgt cgatcattgg gaaggacgtt attttgctta taggaaagga agtaatttcg
    10201 gatttgctac catgcagcta ctcagtctga taggaaaaga aaaaggaaat gttccgagtt
    10261 tggcagaaga agtgctcaca gaattataag aatcactaga acttgtttgg attatgaaga
    10321 gaaaattctg tgaaagctgt tgaaacgatg acaaaatcaa ccactaggca gacataagct
    10381 atacttttat ttttccctat gatagaatac ttgcgactta gacttttcga atgggtgaga
    10441 ggatggaatt gattgaaaaa gcgccagcca agataaattt aggactggat gtattatata
    10501 gacggcaaga tgggtatcat gagttggaaa tggtgatgtc gagcgtagac ttggcagatc
    10561 atctattttt tgaagaaata acagaagaca aaatcattgt agaaacaaat aaagcatttt
    10621 taccggtaga tgaacggaac aatgtttatc aagctgcttt attgctaaaa aataaataca
    10681 atatcaaaac cggtgtcaag atctttgtta agaagaatat tccagtagca gctggattag
    10741 gcggaggaag tacagactgt gcagctgctc taagagggat caaccaatta tggaatctgg
    10801 gctgttcgtt agaagaactg gcagaaatcg gtttggaagt cggtacagat gttccttact
    10861 gtgtctacgg aagtacggca tttgtcggtg gcaaagggga agaaatccaa gtattacctt
    10921 ctatgccaca gtgctgggtt gtgttggtca aaccaagaat gagtgtctct acacgtacag
    10981 tgtttagaga agtagatacg caagccatcc aacatcaaga catccacgga cttgctgaag
    11041 ccatccgaga gcaagattat cagcagatga tcaaaaaaat gggaaacagt ttagaagata
    11101 taacgggaaa gcggcatcca gtcatccatc aaatcaaaga gcgtatgatc aagtacggag
    11161 cagatgctgc attgatgagt ggaagcggac caaccgtttt tgccttgtgt caacattatt
    11221 caagagcgca gagagtatat aatgggttaa aaggtttttg tgaggaagtt tatttagtac
    11281 gaaccctgca ataaaatata agaagtaaaa taggtgatga aaaactgagg aatcctaaaa
    11341 tggtattctt cagtttttta ttgtagtgaa atggctagat atgagcagca actcatccat
    11401 tttacgattt aatcaagaat atacatgctt aaatcagaaa ttgatggatt tctattgact
    11461 gtgttttttt attttgatac attagttcac gttaataaat cgtaaaattc cgatttgttc
    11521 attgaaagga gcatataaat gaaaaaatat cttttgacag ctacggtaat gataggtgct
    11581 ttactgtttg cagcatgtgg aaatacgaac aaggaagcag acaaaaaaga ggatttgacg
    11641 atcgttacga ctttttatcc gatgtatgat tttacaaaag agatcgtagg ggatgaagga
    11701 aacgtgaaac ttttgatccc tgctggaacg gagccgcatg atttcgaacc aagtgcaaaa
    11761 gaaagagcag aaatttccga tgcagatgtt ttcgtgtata atagttcaga catggaattt
    11821 tttgtggatt cgttaaagga ttcagtggat agcaaacaga ccttgatgat cgaagcagct
    11881 aaaggaatcg accgtttaga atcgcaagaa gcagacgaac atgaagaatc agaagaaggg
    11941 catggtcatt cacatgaata cgatccacat gtttggctcg atccggtatt agctataaaa
    12001 gaagtcagaa cgattgctga agaattaggc gaaaaatatc cagataaaaa ggaaactttt
    12061 acgaagaatg ccgatgctta tatcaaaaag cttgaagcac tggatcaaaa atactccgat
    12121 gagctaaaag atgcaacaaa ccgcacattt gtcactcagc atgctgcttt tgcttattta
    12181 gcgaatcaat acgacttaaa acaagtagcg atttcgggcg tttcaccaga tcaggaaccc
    12241 acaccaagtc gcttagctga gctaaaagaa tttgtgaaga aaaacaacat caaagtgatt
    12301 tattttgaag aaaatgcctc ttcgaaggta gctgagacat tgtcgaatga aacaggagtg
    12361 aagctagaag tgttgaatcc tttagaaagc ttgactaatg aacaaatcaa agcaggcgaa
    12421 aattatattt ctgtcatgga aaaaaatctg aaagctttaa aagaaagtat caattaagtt
    12481 ttagaatgga ggttgtaagg tgcgttatat cgaaatatct gatttgacct tttactatga
    12541 tgatgaacct gtactagaag atgtctctta tcatgtagat gcgggtgaat ttgtcatttt
    12601 aactggagaa aatggagcag ccaagtcaac cttggtgaaa gcatcgttag gattactgaa
    12661 accttcaaaa ggaatgataa agattgctga gaaaaatcaa gaaggaaaaa aattaagtat
    12721 cggttatatt cctcaacaag ttgcatcttt caatgcaggt tttcctagta cggtgatcga
    12781 attagtccgt tcaggaagat atccaagagg ccgctggttc aagcgtctct ctgaaaaaga
    12841 tcatcaacat gtacagcgtg ctttagaagc tgtagggatg tgggatatgc gaaatcggcg
    12901 aataggtgaa ttgtctggcg gacaaaagca gcgaatcagt ttagcgcgta tttttgctac
    12961 tgaccctgac ttatttgttc ttgatgaacc aacaacaggt atggatgaag aatccagaaa
    13021 tgaattttat cgtcttctcc gacatagtgc gcatgtccat gggaaatcag ttctaatgat
    13081 cacccatgat catgaagata tcaaacagta tgcggacaga cagatccgac tggtgagaaa
    13141 ggaggattct caatggcgtt gctttcatat gaattcatga gaagagcgtt tcttgccgct
    13201 atcttcattg ccggaatcgc cccgatgcta ggtgtgtttt tagtgatcag aagacaatcg
    13261 ctgatggctg atacgctttc tcatgtctct ttagcagggg tagcgttagg attctttttg
    13321 aaccttaatc ctacacttac gaccatgcta gtcgttgtat tagcagctgt gatattggag
    13381 tatctgcgaa cgatctaccg aacctattca gaaatctcca ttgccatttt aatggctggt
    13441 ggtttggcgc tagcgttagt attgatgaat ttgagcggag ggaattcagc aacaagtatc
    13501 caatcctatc tgtttggttc gatcgtgacc attacatggg agcaagtcat ctttttagga
    13561 atcttattcg ttgccatcgg tatattgttc tttttattca aacggccgat gtatgttctg
    13621 acttttgatg aagataccgc ccacgtggat gggctacccg ttcatttgat gtcgatgatg
    13681 ttcaatgtga ttactggtgt ggctattgct gtgatgatcc cgattgccgg tgcacttttg
    13741 atatccgcaa tcatggttct tcccgcagca atcggtatgc gattaggcaa aagcttcaat
    13801 gtcgtgattg ctatcagtgt ttgtgtagga tttctaggga tgctgtcagg actaactagc
    13861 tcgttttatc tagatacacc tccaggagca acaatcacat tagtctttat tgctttgttc
    13921 ctgattgtaa atatcatcaa acgaatattg gttgccttca gaagaaaaaa taactaaatt
    13981 ataaagaaaa gaagccttat aaaaacgaaa aattttttat aggcttcttt ttattttaat
    14041 aaataccgaa ctattttctt attttttcat aaaatgctcg aatttttgaa gaaaaactta
    14101 tataatgtaa aacgataaaa tttcttaaaa ggagttggaa taggtgaaag tacgtcgcag
    14161 tgaacgtcta gttgacatga cacagtattt attgaatcac ccgcatgaat tgatttcctt
    14221 aacaagcttt gccgaacgtt atgaatctgc taaatcttct atcagtgaag atttgggaat
    14281 catcaagaaa acatttaaag aacgagggta tggaaccttg gaaacaatcc cgggtgctgc
    14341 aggtggtgta ttgttcatcc ctgaaatttc atatgaagag gcgaaaaaat atattgaatc
    14401 tctagcagaa cgtctatcag aacaagaccg tctgcttcca ggaggatatg tttatctctc
    14461 tgatttgtta ggagaacctg aactgctgcg tcaagttgga agaatcattg cttctaaata
    14521 ccttggtgaa aaaatcgatg ctgtaatgac tgtagccaca aaaggcgtac ctattgccca
    14581 agctgtgtcc tattatctga atgcgccatt cgttattgta cgccgggact caaaaataac
    14641 agaaggatca acagtaagtg tcaactatgt gtctggatct tctgaaagaa ttgaaaagat
    14701 ggaactgtct aagagaagtt tgaaacgtgg ttcacgtgtc cttgtagtgg atgatttcat
    14761 gaaaggcggc ggaacagtca acggaatgaa gagcttgatt gaagaattcg aatcagaact
    14821 tgttggcatc actgtatttg ccgaatcaaa attcaacggt catcgcgcca tcgatgatta
    14881 tacgtcactg ctttatgtca aagatgtaga tactcaaacg aaaaatatta cggttgtgcc
    14941 aggaaattat ttttcagaat aagaggttgt gaaaaagccg cttagtttcg agtagtaaga
    15001 aaaattttgt gaaaatggct ttccacattt tttcataaat ttggcttaat accgagaaac
    15061 tggcttttga acaccgttta tctagaggtt gtgaaatcag cgtcctgtcc tgagtattag
    15121 ctcaataaac ggaaaaattg ctcctcatat tttgccgttt attgagctaa taccgaaggt
    15181 cagctgattg aaaaacgttt aatgaagagg ttgtaagcct accgtttaac tctaagtcac
    15241 aagaaacgat ttcaaaaaac agcttttcat gttttatgag tagtgtgact tgtgaccgag
    15301 gagttggcag gagagcaccg gttaccaaat agataggccc gttatggtct atctattttt
    15361 ttatccagaa aacgctggga taaaagaaaa tcacttgttt tgttgaaaaa atgggtatac
    15421 tgggataaac tgaaatcagc aaactatatt taggagtgac gcagttggac gcacgttatg
    15481 caattatttt ggcggcaggg aaaggtaccc gcatgaagtc aaaattatat aaagttttac
    15541 atcccgtttc tggtaaacct atggtagaac atatcattaa tcgggtcagt gaaacgaaac
    15601 cagatgaagt gattacgatc gttggacacg gagcagaaca agttaaagca caactaggag
    15661 aacgcagtaa atatgcgtta caagcagaac aattgggaac aggacatgct gttttgcaag
    15721 ctgcttcttt cttggaagga aaaaaaggaa caacgttagt tatcagtggc gatacaccat
    15781 tattgactac tgaaacgctg aacaatcttt ttgaatatca ccaaggaaaa aatgcaagtg
    15841 caacgatctt gacggcacaa gcagaaaacc caacaggata tggacgaatc atccgtgacc
    15901 atattgggat cgtagaaaaa atcgtggaac aaaaagatgc aacaccagaa gaagctcttg
    15961 tacaagaaat caatacagga acttactgtt ttgacaacga agctttgttc gatgcgttaa
    16021 gcaaagttgg aacaaacaat gctcaaggtg aatactatct aacagatatc atcgaaatct
    16081 taaaagaaga aggccataca gttgctgctt atcaaacgga tgattttgaa gaatcaatgg
    16141 gagtcaatga tcggatcgcg ttagctaaag caaatgagat catgagaaag cgaattaacc
    16201 agatgcatat ggtcaatggc gtaagctttg tcgattcagc aacgacatac atcgatgcag
    16261 gcgtagaaat cggtccagac acattgatcg aagcaggtgt acaaatccaa ggaaatacag
    16321 tcattggttc tgattgtgtc atcgggtcac attcaaaaat cgtagacagt aggattgagg
    16381 atcacgtcgt cattgaaaac tcagtcattg aaagcagcca tgtcaagaaa catgcagatg
    16441 tgggacctta tgcacattta cgaccaaaag ctgaaattgg cgaaaacgtc catatcggaa
    16501 actttgtgga agtgaaaaat gctcaaatcg gcaaaggaac taaagtcggt cacttgactt
    16561 atgttggtga tgctacgctg ggagaagaaa tcaatgtcgg atgtggtgtt gtcttcgtca
    16621 actatgatgg aaagaacaaa catcatacga ctgtcggcga ccacagcttt attggttcta
    16681 gcacgaatat catcggacct gtcgaggtag ctaaaaacag ttcgattgct gccgggtcaa
    16741 caattacaga caatattcca gaatatgcgc tggcaattgc gagagcaaga caagtcaata
    16801 aagaaggata tgcaaaaaaa ctcccttatc taaactaaga cttttgcaaa atcacttgat
    16861 ttatttaaaa aatatgacta ctatttatta ggtagaattc tagagttgtg aaaaagaaag
    16921 tggaggtccc catgtcaaaa cattactttg acccaagatt aaaaattttt gcgttaaatt
    16981 ccaatcgccc attagcagag aaaattgcag cagcagtcgg cgtcgagtta ggaaaatcat
    17041 ctgttactca atttagtgat ggagaaattc aagtaaacat tgaagaaagt attcgtggat
    17101 cgcatgtcta tgttattcaa tcaacgagca gcccagtcaa tgataacttg atggagttgc
    17161 tgatcatgat tgacgcactg aaacgagcaa gtgcaaaaac aatcaacgta gtcatgcctt
    17221 attatggcta tgcgcgtcaa gaccgtaaag cacgtgcacg ggaaccaatc acagcgaaat
    17281 tagtagcaaa catgattcaa aaagctggtg caacaagaat gctgactttg gaccttcatg
    17341 cagtccaaat ccaaggtttc tttgatattc cagtcgatca tttgatgggt gcgccactta
    17401 ttgccaatta tttcttagaa aaaggaatca aaggcgatga tgttgtcgtt gtttcaccag
    17461 accatggtgg tgtcacacgg gcacgtaaat tagcggaatt tttgaaatct ccgattgcga
    17521 tcatagacaa acgccgtcca aaagcaaatg ttgcagaagt aatgaatatc attggacaag
    17581 tcaaaggcaa aacttgtgta ctgatcgatg atatgatcga tacagcaggt acgatcacat
    17641 tggctgccaa tgctttgaaa gaagctggtg caactagtgt gtatgcttca tgtacacatc
    17701 cagtattatc cggccctgct ttacaaagaa tagaagattc agctatcgag cgtctagttg
    17761 ttacagactc gatctatctt ccagaagatc gtaaaatcga caaaatcgat gaagtaagtg
    17821 ttggcggact aatgggagat gcaatcaaac gtatccatga aaacaaacca gttagtccat
    17881 tgtttgaaac aaaaaataaa taataaataa actcacaaag gggagtccaa ttgggctgag
    17941 attgaatcta tttctaaacc cttcgtacct gtatcggtta tgcgagcgta ggaattgtga
    18001 ataaacagct gtttgactat tttcgaaagc tgcttcctcc tttgtgactt ttttattcaa
    18061 aggaggaagt ttttttatgg gaaaaaatag agtttgggta gaaggaacga ttgttgcagc
    18121 gttagcgatg gtgttgtcgt tgattccgat ccagatcggc agcagttttt ctatttcatt
    18181 agggcagatt ccattgactc tttttgcgtt aagaagaggg acaaaggcag ggatgctggc
    18241 aggtttcatt tggggcatcc tacattttcc gttgggacaa gtttattatt taagtgtcgt
    18301 tcaagtgttg attgagtacc cgattgccta tacttttgca ggcgcagcag ggctcatggc
    18361 ttcaaatgtc caaaaaggct atgtagacga taatacgaaa caaattagaa aaagcatcgt
    18421 gtttggtgca ttgattggcg cactttccag atatttttgg cattttattg caggagtggt
    18481 tttctgggga gcgtatgcac tatgggggat gaatccttgg ctcttttctt tcgtaatgaa
    18541 tggtgccagc gggttgagta cagctattgt aacgatcatc gttttattag ggatccaaca
    18601 aacgactcct tcgatttttg tgccagataa acgacaggag ctgtaaataa aaaagaagca
    18661 agaaacttga gacagaatca tgtcctagtt tcttgtttcc tttttatttt ttgataagaa
    18721 atagatctac ttcaatggca gataagaaag atcggcatct tctaccaatt gccagtgtcg
    18781 atcggtgaca gagtagatta aagtagcgat gatttcctct tctcgatacc gggtgccttt
    18841 taatttccaa atataatctt caatcacata gattgggata cgatatttac catcgatcaa
    18901 accaacttga tcaaaagaaa aaatgatccc ttcctgcatc aaattacgca tttctttcaa
    18961 taattccaca ctgatttttt caacaggtgt ttcttttttg aaaaaagatt tttttacatt
    19021 gatcttatct gacaagatcg aacttaattt actagcaaaa atcctataaa attcatctaa
    19081 atagcgataa taaatccgat tcagctgatc attaagatcg aagtatacaa aattattctg
    19141 tagtttataa aaaaacggcg aatgaagatg tgttttcata tgaccaaaat ataataattc
    19201 tgaaatttct aaaggtgtca attctttcag catcgttaaa tcagtaaagt cgatccactt
    19261 caattcatcg aatccacgcc tgttacgaga agaggaaaag aaacgctgaa tattttctgt
    19321 accacggatt actttcagcc ctgtatggga ttcatattct ccagcagaag cagatggatc
    19381 aagcaagagc aagttttgag gaaaatgcac gatactgttt gcaaagtcag tagcagtcag
    19441 cccttttgtt aaaacagcat tacttgtggt atcaatatgt acataaactg actcagtcat
    19501 catgttcctc ctcttatttc ctcttaatct cttttatttt acaacatttg cattaaaaaa
    19561 gcttgtttct tttattttaa aattccatta caaaatcacg ttttcattga aaacactctc
    19621 attttcaatt aatttttgtg atttttgtat ttttttattt aaaggaggtt ttatgaattg
    19681 ttttcttatg gaatctacaa agtcgaatac gagactcatg acctttttat gacgtactgg
    19741 tatacttgaa gaaaaaggag agcgcgatgg agataaaaaa agtttacttt tcacagaaaa
    19801 tgtcgctgta ttcgcctatc cagcaagcaa aacaacccct tccatttttt caaagtcatc
    19861 aagaaagtag agacgctttt tggcgttctg caaaaaagca atgtgctttt aaagcagtat
    19921 cgaaaaaata taccgtctat tttactattg ctaaaagtag aggagatgtt atttttaata
    19981 atctgttgat tgaaggtgaa acttttgaat ggaaaaaatt ttttcggtat atggaagctt
    20041 gcgctcgttt ttttattaaa gaaaactgtt gttttttatt tcaatctcct ctatcagaag
    20101 aatggttgcg tatttttcat aaaaacggct atcaaggaac cacacaatta aataaaaagc
    20161 tggtctatca tacagcactt gttttaggtg gaggaggagc gcacggtgct tatcagattg
    20221 gtgtgtggca ggcgctgaag gaacatgata tcaactttga gattatcaca ggtacctcgg
    20281 taggtgcgtt gaacggcgca ttgattttac aaggagatat gaaaaaagct gtgaatttat
    20341 ggaaaaagct gtcgacaaga caagtgcttg cgttaccaga aatggctgca gttgaagacc
    20401 ttcgtgaacg cttctatcga gaaagacgcc aaatgacgaa aacagcacta attgaaggcg
    20461 gggtttcttc tgctccattg gaaaagatgg tcaaagacaa tttggatttg tccttactca
    20521 aacataatcc caaaatacgt ttatatacgg tgtctactaa actgccagaa ctagcggaag
    20581 tagtgactga tatccaacag acaaaaacag aagaaatacc agattggata ctcgcctctg
    20641 cttcatttta tcccgctatg gcttatcgaa agatcggaaa aaataaatat gcagacggtg
    20701 ggtatcgcaa caaaatacct attgatattg cgattaacaa aggagcaaca gaggcatttg
    20761 tcgtagatgt gcaagggcca ggcccagcga aaaaaattcg tatgccagac atttttatcc
    20821 attggaaatg tcaaacattg tggacactag gcagctttct gctttttgat agccaaagga
    20881 accaattaaa tttacaatta ggctatctgg aaatgaaaaa acgattaggc tattactatg
    20941 ggaactggta tacatttcat tctgttaaac ctgcaagaac atactggcga ggatttttgt
    21001 cttacttaac aaaagaaatt cagttagaat tatctttttt caaaagcata aaattttggc
    21061 aaaaactgcg taatttatat aagaacaggg tcgtaccaga gacatgcgga ttggcgatgc
    21121 tagaactttt agcaaaaaaa cattttttac tgcccaatga aatctatcaa gtagacatga
    21181 tgattcaaat gatctgccaa caagatataa aaagtatgcc ggatgattta ttggcacaaa
    21241 ttggtcaatt aagtactgaa gaatggcgaa ggtatcgaaa ataccagcaa aaaatacaaa
    21301 atgagcgcac gaaaactgtt tattttgatt atctgctaca aggaaaacgc aaggatcgtt
    21361 tgcagcatga attgcttaag caaccagttg acacattact gatcctatat ttatattatc
    21421 tcaaggagga acaaccatgg cacaagaatt ttcatatgaa atcgtagaag aaatcgctat
    21481 attgtcagaa aacaacaagg ggtggcgtaa agaactgaac ctagtcagct ggaatggtcg
    21541 cccgccaaaa ttcgatcttc gagattgggc gccagaccat gaaaaaatgg ggaaaggcct
    21601 gacattgacg aacgaagagt ttgaacaact gcaaaaagca atagaaaata tgtaatgagg
    21661 aaggaccctg tcgaatgaaa agtatgactg gttttggcaa agccatccgc gaaacaaaag
    21721 cgtatcagtt agatattgaa ataaaaagtg tcaaccagcg ttttctagat gttcaaatac
    21781 gaagtcctaa aatcctgaac tttgttgaaa acgattttcg tcagcttatt aaacagcagt
    21841 taagccgcgg tcgaatcgaa gtatttgtga atctagctta tattggcaaa aacgaaaaac
    21901 agatcgtcat caactgggaa ttgattgatt cacttatcac tcaattgaca gacggaattc
    21961 aacaacgata cggtagtcaa aatcaagtgt caattagtcg gatggcggaa cgcttcgtgc
    22021 tggacgaaaa cttcgtaacg gtagaagaaa aaccggaaga gagcatggaa gaattggcac
    22081 agttagctct agatacattg aaaagcgcac ttgattctat tgaggcgagt cgagaaaaag
    22141 aaggtcaagc tttagcttta gtgttgaaaa agaatactga agaattcaaa gaagtactaa
    22201 aaaaactcga tagttttgta gaaatctatg aaaaggaatt tcaaataagg tatcaaaaaa
    22261 agctggagga atttcttgga gcgaaagtcg atgagcaacg gttgttgacg gagctagcca
    22321 tcttgttgga acgcggagat attcacgagg aactggatcg tctggcgatc catgtacaaa
    22381 aactagatga acttatcgaa gcacaatgtc ctgtaggtcg ggaattagat ttcctgatcc
    22441 aagaaatgaa tcgggaaatc aacacgatcg gttccaaatc aaatgcaatc gaaattaaaa
    22501 atcaagtcgt ccagtctaag acgatcttag aaaaaatcag agaacaagta caaaatattg
    22561 aatgacgaaa gaggtagaga taatgaaata ttatgacgta acgttccacg aattaagtgg
    22621 aaaaactgtg gttaaacggg atatcccttc tgaaaaaaac ggctttgacg tatggaaaga
    22681 tgcctgtgca gactataatg agaacgaact gtttattttg atcaacgacg gtgcttacgt
    22741 gacgatgaat cggaaattta ttgttcgaat tgacacagaa gaggtagaag atccgacaga
    22801 aaaagcacgt tctcgaaaag atgagatcat gggtgtagtg aatactttgt ccaatatggg
    22861 cttttagcaa ggatgctgaa caaagaaata caataaataa acagaattgg atttcttcgc
    22921 ttagcttctt tgctctagga atacatttaa aggttgtgac aaaattcgtg tcacaatctt
    22981 tttttatttt gaataagtga tgttcaaaaa ccattttctc ggtattaagc caaatttatg
    23041 caaaaatgtg agaaactatt ttcacaaaat ttttcttcat actcgaaact aatcggcttc
    23101 ttcacgacct cttatacacg gcgttcaaaa gcttgcatct gcgataataa ccccaaccaa
    23161 acgaaaaaca tgagaagctg tttatgtttg gctgttctta ttacttgatg caggacagct
    23221 tttttacgac ctcttggtac agggtagctt ttttaccaat ttctagctat aattaaagat
    23281 aaccttgaaa gttgcagaaa aaaagtgcaa aataaacgat aagtacaaaa agactagttg
    23341 aaaggacgtc gtcatgtcag agcgtggatt attaatcgtg ttgtctggtc catccggtgt
    23401 aggaaaaggc acagtacgaa aagcaatttt tgagagtgag gataatgatt ttcagtattc
    23461 gatctcaatg actacccgta aaatgcgtga aggggaggtc gaaggcgttg attatttctt
    23521 ccgaagcaaa gaagagtttg aatcaatgat cgaagctgga gagatgctgg aatacgcaga
    23581 atatgtcggg aattattatg gaactccttt gtcatatgtc aacaagacat tggatgaagg
    23641 aaaagatgtt ttcttggaga ttgaagtcca aggagcaaaa caagtcaaag aaaaagtgcc
    23701 cgatggtgta ttcatctttt tgactccgcc agatttagcg gaattaaaat cacggatcgt
    23761 aggccgagga acagatgcag atgaagtaat cgaaaaacga atgaaagttg caaaagaaga
    23821 aatcgaaatg atggctcttt acgactatgc tgttgtaaat gatgaagtgc cgctagcagt
    23881 tgaacgaatc aaaaacatta ttgccagtga acattttcga gtagatcgcg taatcggtaa
    23941 atatattaaa atgttaaagg agatgtagtc ttatgatgtt aaaaccttca attgactcac
    24001 tattagatcg tgtaaattca aaatattctc tagtgatttt agcaagtaaa cgcgcgcatg
    24061 aattggatgc aagtgcacag cctactctag attcttttga ttcagtaaaa agcgtcggtc
    24121 aagctttaga agaaatcgat gcaggaaatg tagtcaatga tccacatcct gaattaaaac
    24181 gtgaacgttt gaagatggaa gaagaggaac gtcaagcaca aaaagagcgt gaacaacacg
    24241 aattggaatc acgtatccgt gaagagcaaa aaatgtaata aagaaaaata gagcgaacat
    24301 ctaaaaagaa aagcaaaccc agctgtttcc ttttcaaaca ttcgccactt attgtctggt
    24361 agaaaaggct gtgtctgaac tttttgacac agttcttttt tttcttatct gctgttaatt
    24421 ttttgttagg atttggcaga agataagaca aatccatcga tctctagtaa aataggcaag
    24481 gaactttttg tcgcttgata agaaaagaca aaaaaaggag gaaacgaacc atgacattaa
    24541 cagctgaagt cattgtggac gtacctacta tgcagaccga tcagccattc acttatttgg
    24601 ttccctctga aatggaaaca gctttgcaag tcgggatgcg agtagaagtc ccttttggta
    24661 atggcaatcg tcatgtccaa ggatttgtta tggctatcca aaaatcagaa gaatcagcta
    24721 atccatcttt gaaggcagtt attcgtttgc tagaccttgc tccggtggtc aatgaagaat
    24781 tactttcatt agctgattat atgaaaaaaa ttacttacgc attcaaaatc acttgtctac
    24841 aaacgatgct tccgagtgtg atgaaagcag aatatgataa attgatctat cctttggcag
    24901 acacaccaga agttcaggta ttgtttgatc agcgagaagt gatttcctgg aaggaagcag
    24961 aagaagaagg cagcctttct caattgattc gctggcgcca agagcaatta gtagatatca
    25021 aatatgaagt ccatacacga aataaagtaa aaacgattcg tttggtccgt tctttgttaa
    25081 ctgaaaagca aatcgaagaa gaatgggcaa aattacggca aaatgccaag aaacaaaaag
    25141 aacttctttt gtgtttatct gaactgagcc aagaagaacc aattgcttat ttcaaaaata
    25201 aaggaatcag tactgctgtc ttgaatcaag gaaaagaaaa gggctggctg gagtttatag
    25261 aatcagaacg ttatcgcgac ccttataaag accgagtatt tgaccaaacg acggcattgg
    25321 aactgaatgc agaacaaaaa aatgctgtcg aacaaatcat cacagcaggt cagcagcaaa
    25381 aagatcaggt ctttttgctg gagggaatca ctggcagcgg gaaaacagaa gtatatcttc
    25441 aggcaatcgc tgatgttttg tcagaaaata aaacggctat tatgttagtg cctgaaatcg
    25501 cattgactcc gcaaatggtc gagcgattca aaggacgctt cggagaatca gttgcggtct
    25561 tacatagcgg actttcccaa ggtgaaaaat atgatgaatg gcgcaagatt gagcgggaag
    25621 aagcgcaagt agtagtaggc gcacgatctg ctatttttgc cccgctgaaa aatatcgggc
    25681 tgatcattat cgatgaagaa catgaatcaa gttacaaaca agatgagaca ccgcgttatc
    25741 atgcgagaga tctagcaatt tggcgcagta aatatcatca ttgcccaata gttctaggaa
    25801 gcgcgacgcc ctctttagaa tcaagagcac gagctcaaaa aggcgtttat cagttattgc
    25861 agttaaacca ccgagccaaa gcagctgcac agcttccagc tattgagatc gtagatatgc
    25921 gtgaagagtt tcagaatcat cggacctcta ctttttctgc taatttgcag gaaaaaatac
    25981 aaaatcgatt agacaaaaaa gaacaaactg tcctactgct caatcgtcga ggatattcgt
    26041 cttttgtgat gtgtagggac tgtggatttg ttcttccatg tcctaattgc gatatctctc
    26101 ttacactgca tatggataca cgttcaatgc gctgccatta ttgcggacat gaagaaccta
    26161 ttcccaatcg ctgtcctaat tgtggcggca ataaaatccg ctactatgga acaggtactc
    26221 aaaaagtaga agaggaactc cgagaacttt atccgcaagc taggattttg cgtatggatg
    26281 tagataccac tcgaagaaaa ggggcacacg aacagatttt acaaaaattc ggtgcaaaag
    26341 aagcagatat attattaggg acacaaatga ttgctaaagg tttggacttt ccagaagtga
    26401 cattagttgg ggtgttgaat gctgatacct ccctgaattt accggatttt cgttctagtg
    26461 agcatacatt ccaattgctt acccaagttt caggacgagc agggcgagcg gaaaaagcag
    26521 gcgaagtcgt gatccaaaca ttcaatcctc aacattacgc catagaattg gctaaaaaac
    26581 aaaattatga gcagttttat cagcaggaaa tgcatgtacg gcatcgtgga ggctatcctc
    26641 cttattactt tactgtgaaa ataaccgcca gccatcctga agaacaagta gcggcaaaga
    26701 aaatctttca gattgccaat cagctaaaag aagtgttaag ccctcaaagc ttgctattag
    26761 ggccaacgcc cagcatgatt ttgagagtga aaaaccgata ttattatcaa ctaatcatca
    26821 aatataaaca tgaaccgaat cttcctcatg ttttggatga aattttgaat ggaagccaga
    26881 aagaacagcg acaaggtctt tttgtggcaa ttgacaacga accattaaac tttatttaag
    26941 aaagggccaa attatgcgct atcctatatt gatccaccct aatgataaat taaaaagaac
    27001 tgctcagcca atcgatgtaa tcacggacga gacaatcgct ttattagaca atttatatga
    27061 gacaatgatt gcgaatgacg gaatagggat cgctgctcct caagtaggac aaaacaagag
    27121 aattgctgtg atcgaagtag atgaaggcga gaaatttgaa ctgattaacc cagaaatcat
    27181 cgaagcaaaa ggtgaaagcc ttgacgtaga aggttgtctc agtattccgc atgtctatgg
    27241 aacggtcaag cgagcagatg aagtgaccgt gcgttattat gatcgagacg gagaagagat
    27301 agaagtgacg gcttttggct atttagcccg tgccttccag catgaaatcg atcacttaga
    27361 cggtatcttg ttcattgaga aaatgataca gcagatcccg gaagaagaac ttgaagaata
    27421 tatggaggaa catcttgatg actaagctag tatttatggg cacacctgct ttttctgtgc
    27481 cgattcttga agggctgata gaatcaggtt acgagatcca ggcagtggta acacagcccg
    27541 accgtccagt tgggcggaaa cgagtaatta cgccgacacc ggtaaaaaaa gcggctctga
    27601 agcacggtat ccgtgtgctg cagccagaga aaatctccgg ttcgccagaa atggaagaaa
    27661 tcattgaatt ggtgcctgat gtgatcgtca ctgcggcatt tggccaattc ttgcctgaaa
    27721 agttattgca agtaccaaaa ttaggggcaa tcaatgtcca tgcttcttta cttcctaaat
    27781 atcggggagg tgcgcctgtt cattatgcca tcatgaatgg ggagaaagaa actggtgtaa
    27841 cgatcatgga aatgatcaaa aaaatggatg caggcggaat ctatgcgcaa gaaagcatac
    27901 caatcactaa acaagatgat gtagggacga tgtttgaaaa gcttagcctt ttaggaagaa
    27961 agctattgct agaaacattg cccaatattt tggatggaca aaaaccagta cctcaagacg
    28021 agtcagaagc aactttttcg ccaaacatca cacgggaaca agaagcaatc gattggcata
    28081 aaactgcaga agaaattgac aaccaagtac gcggaatgcg tccttggcca attgcattca
    28141 cgacctatga gggcatacgc tggaaattat tatcggttga agtattggca gaaactacag
    28201 acaaagcacc tggtacgatc atccggaaag acaaaaaaaa tctatggatt gcttgcggta
    28261 aacagaccgt cttagccatc aatgaattac agccagctgg taaaggaaaa caggccgtac
    28321 atgatttcct taacggaagc ggtcagcaag ttcagattgg tcagcaggtg aaataatgaa
    28381 aaaaatccct aaaaaaatga aaaaatcagt acgatatatg gcattactga cattagagcg
    28441 agtgaacaag ggtggcgcct actcgaatct tttgttgaat gagatgatca aacaaagcga
    28501 gctaaatcaa aaagatgtgg gtttatttac agaattgatc tatggtacga ttagccatca
    28561 gcgactattg gaattttacg ttgcaccatt gatcgctaaa gcaaaaaaag tggatgactg
    28621 ggtcaagatg cttctttatc tttctgtata ccaattagaa tttttagata aagtccctgc
    28681 acatgctatt ttaaacgagg cggtcgaaat cgcaaaagta aaaggaaatc ctgggacagg
    28741 gaaatttgta aatggtgtct tgaggaatta tcagcgtcaa ggagcaccag atctgcaagg
    28801 cattgctgat cctatcgaaa gactttctgt caaaatcagc ctgcctctat ggatgacgaa
    28861 acgactggtg gatcaaatcg gatacgaaga aactgaaaag ctcggtttat cgctatatca
    28921 gcctagccat gctagtgccc gagtagatac acgccgactt tccagagatc aggccatcac
    28981 tgttttgcaa gaagaagaga tcacagctga aaaaagccaa gtcagtccat acggtatagt
    29041 agcgaaaaaa gggcatttag cttctagctc attgttccac gatggcgtca tgacgatcca
    29101 agatgaaagt tcgatgcttg tagcgccgtc tatgcaaatc gagcctgaac atcatgtctt
    29161 agatgcctgt gcagcacccg gaggtaagac gacgcatatt gcttcttttt tagatccgtc
    29221 atgcggtgga gaagtggtcg ctttagatgt acacgaacac aaagtcaaac tcattgaaga
    29281 taatgcagca cgtctaggtg tctcttctgc tgtctacgct caaaagatgg atgctagaga
    29341 agtcagtaaa cagttcgaag cagagcagtt tgatcggatt ttagtagacg cgccttgttc
    29401 aggtcttggg ctgcttcggc gaaaaccaga tattcgttat aaaaaacagc cggatgaatt
    29461 agcgaattta ccaaaaatcc aattggaaat tttagaaagc tgcgcaccaa ctttaaagtc
    29521 ttccggtatt ctgacttata gcacttgtac gatcttaaaa gaagaaaacc aagacgttgt
    29581 ttctgctttc ttggaaagac accctgattt tgagaggatt gatgttttga ccaatcctat
    29641 gctacaatca tcaatcgaag agaaaatgtt gactttgtat ccgcatcagt tttacacaga
    29701 tggatttttc atttgttgtt tgcgcaaaaa gtaatgaggt gaaaaaatgc agattgaata
    29761 tcaatcagat gtaggaagac gacgtaacac aaatcaagac tatgccagtg tatttaccag
    29821 ccgttcaggt ctcaagcttg ctgtattagc agacgggatg ggtggtcatc gtgccggaga
    29881 cattgccagt cagatggcgg taacgaatct aggcgaagct tgggaaaaac aagaagctgt
    29941 tgacgatgaa aaaatcgccc aatggttcat tcaatcgatt caagaagaaa atatgctgat
    30001 ttatcagcgt ggacaagaac agccggaata taacggtatg ggaacaacaa tcgtagcggc
    30061 cgctctgtca gatgactggt tcacgattgc caatgttggg gatagcagag cttatttgat
    30121 tcgtgaagat aaaatcatgc aattgacgga ggatcattct ttagtcaatg aactagtcaa
    30181 atcaggagaa atcagcgaag aaatggcagt gaaccatcca aggaaaaata ttttaacacg
    30241 ttctgtcggc atgcctggaa cagtcgaagt agatgtttct actcatcttt ggcaactaaa
    30301 agatcgactg ctgttatgtt cagatggctt gacgaacatg ctgtctgaag aagaaatagg
    30361 aacgatcatc aatcaagaag gcccattgtc tcataaagta actgagctga ttgatcgtgc
    30421 caatgaagca ggcggagcgg ataatatcac cgttcttctt atcgaatata aggaggacag
    30481 tgtatgatcg agttgggcaa aaaattaaat ggccgttatc aaatcactgg aaatatcggt
    30541 agcggtggta tggcaaatgt atttttggcg cacgatttaa tcttggatcg tgatgtagca
    30601 gttaaagtct tacgatttga ctttcaaaat gaccagactg cgattcgacg atttcagaga
    30661 gaagctttag cagcaacaga actcgttcat ccaaacattg ttagtgttta cgatgttggt
    30721 gaagaagaca acatgcagta tctggtgatg gaatacgtca aaggaatgga cttgaaacga
    30781 tatatccaaa cacattatcc tgttccttat gaaacagcag taaatattat gcagcagatc
    30841 ctttctgcta tttctttagc tcataaccat caaatcatcc accgagattt gaaaccccaa
    30901 aacgtactga tcgataatga aggtgttgta aaaatcacgg atttcgggat tgctatcgct
    30961 ttatctgaaa catcgatcac gcagacgaat acgatgctag gatctgttca ttatctgtcc
    31021 cctgagcagg ccagaggaag tatggcaacg aagcagtcag atatttatgc tttagggatc
    31081 atcctttatg agatgttgac gggtagcgta ccgtttgatg gtgaatctgc tgtaacaatt
    31141 gctttaaaac attttcaaga tgatcttcca tctataaaag cacttgatcc aaatgtgcca
    31201 caagcactgg aaaacgtgat tttacgggca acggctaaag aacctgcaga ccgttataag
    31261 tcagccgagg aaatgtctga tgatctttct acagtattat caccagctcg tgcaaacgaa
    31321 gaaaaatggc agcctcatgt gatggataat gaaacaaaag ttatcacacc ccttacggaa
    31381 gatgcaccaa tgcctgattc attcaaatct atgcctctgc caaaagacaa gccagaaaac
    31441 gaaaccttac cagaggagga agaagttctg cctgatccta aaaagaaagg acagaaaaaa
    31501 tggtggctga tcctattagc tattcttgct gttttaggaa tcggaacagc tgtattcttt
    31561 gcttctggcg ggcgaggaga agttgttgtt cctgatgttt ctgatctttc tgaagcttct
    31621 gctagagaaa cactcgcaaa agcgaaatta gaagtagcaa caaaaacaga agaaattgca
    31681 gatgacaaaa tcgaagaagg taatgtagtg aagacagacc cggcagcggg aacgacagtc
    31741 aagcaaaaac gcgaagtcac tctgtatatt agtagcggca cgaaaaaaat caaattagat
    31801 gattacagtg gtaaaagtta tgaagatgcc gaagcaagtt tgaaagaatt agggttttct
    31861 gctgacttga ttcaaaaaga agaggaattc agttcagagg tagaaagcgg aatgatcatt
    31921 tcgcaatctc catcagaaga tacagaagtc gatcctaaaa cagacacgat tactttcaca
    31981 gtcagcaaag gaccagaaac agtgactttg agtagtcatg taggagaaaa ctacgaaact
    32041 gttcgtaatt ggcttgttac acaaggattc aatatcaata atattagtgc tacttatgac
    32101 tatagcgaca ctgtaccatc aggttcgatc atcagtcagg atccagcctc gggtgaagtt
    32161 gtagcagaag aaacttacat caattttgtc gtaagccaag gaacagagcc taaattgaat
    32221 gatatctccg gctatacaaa aagcgaagca caaagttatt tagctagtgt cggtgcagaa
    32281 tatatcgggc atgagacttc cgaatattca aacacggtgg ataaagataa agtgattcgt
    32341 acagtaccag gggcaggaac gacaattacc aaaggaatgg ttgtgaacgt aatttactct
    32401 aaaggaccag atccagcgtc ttctagttct gaaaaagact cttcagacag ttctagcagc
    32461 tcaagtagct ccagtagcac aagcaactct tcaaaagaag aatcttccag ctccacaagt
    32521 tcatcagcta gcacctcaga aagttcaaaa taagtgctga ataaaatata tcagaaggga
    32581 atgcgacatt gatttgttgc attctctttt ttgtgtttct atctcttttg atcaattttc
    32641 aagccatata ctttgagaag tttgcatgga ataacaccta ctttcctctt cgtatggtaa
    32701 aataaacaaa aacggaggtg gcacttatca aaggacaaat cagaaaagca atcagcggtt
    32761 tttactatgt atacgcaaac ggagagacct atcaaacaag aggaagagga aatttccgga
    32821 atcggaaaat cacgccgctt gtcggagatc aggtgatctt tgagagtgaa aatcaaacag
    32881 acggctatct tctggaaatc ttgccgagaa aaaatcaatt agttcgccca ccagtcacaa
    32941 atgttgatct tggaatcatt gtcacaagta tcgtagagcc tgcattttct tataatctac
    33001 ttgaccgctt tctagttacc ttggaatacg agtcgatcga accgattatt tttttgacaa
    33061 agaccgactt gtcagacgac ccagaaaaaa tcgaagagat caagcaaacc tatactaaaa
    33121 taggctatcc ggtgattgta cccaatcata cgggtgatac gacagaactg atccgttact
    33181 tcccagaacg gctatcggtc tttatgggtc agtctggagc aggaaaatcg acgctactga
    33241 atcaaatatc accggaactg aatttagcaa caggagagat ttctgatgca cttggaagag
    33301 gacggcatac aacacgtcat gtggaactga ttcctatcta tggcgggtta gtagcagata
    33361 caccaggatt tagctcgatt gactttttga caattgaagc aggagaactg ccgagacaat
    33421 ttcctgaatt tgtagaagct tctgtccatt gcaaatttag agagtgcatg catgctaaag
    33481 aacctggctg cgaagtaaaa catagagtag aaacaggtgg tatcgcacag acacgttacg
    33541 acaattattt gcaattctta caagaaatcg aaaaaagaaa accaatgtat gcaaaaaaag
    33601 aaggtaaacg aaaataggag gaaacaagaa tgaagatagc accatccatt ttgagtgcag
    33661 attttgccaa tttacaaaga gacattgagt tagtggaaaa aggaggcgca gattacatcc
    33721 acgtagatgt aatggatggc cgatttgtcc caaatatcac attagggcca aatattgttc
    33781 aggcaatccg tcctgttact aaattaccgt tagacgtcca tctgatgatc gttgatccag
    33841 aaaactacat tccagcattt gcaaaagctg gagcagatat cattactgtc cacgtcgaag
    33901 caactcctca tattcatcgg gcacttcaaa tgacaaaaag cttaggagtt aaagctggag
    33961 tagttatcaa tccaggaaca ccagtctcca tgatcaaaca tgttttgcct ttggctgatc
    34021 aagtactagt catgactgtc aatcctgggt tcggcggaca aagctttatt gaagaaacag
    34081 tagaaaaaat tacggaactc gctgatctac gtagagagaa caattggact tatgcaatcg
    34141 aagtagatgg cggaatcgtt ccagaaacag caaaaatctg taaagatgca ggggcggatg
    34201 tcttcgttgc aggatcatat atctatggag ccgaaaatcc agtcggacaa atcgaacgat
    34261 taaaagaggc attagactaa atgaaaattt tattagctgc tggaggtaat ccgcaaaact
    34321 ggccgcagtc tctgctgtcc aaatacgatg cctatgtcgg gatagaccgt ggtgcgctgt
    34381 acctgcttca agcaggctac ccagtagatc tggcagtggg tgattttgat tcattgtcga
    34441 acgaagagca gaaggaagta tttgcaaaag ccaaagagta tacccgttcc cctgcagaaa
    34501 aagacgatac agatacacag ttaggtatct taaaaacatt tgaacgttac ccgcaagcgg
    34561 aagtcacttt gattggagct acgggcggaa gattggatca tcttttagcc aatctctggt
    34621 taggtatgga acctcgcttc aaaccctttg cccatcagct tattatcaga gattgtcaaa
    34681 attatttgtc ttattatttg cctggagagc atatagtaag acagatacca gatatgaagt
    34741 atttagctta ttgctgcttg actcccgtct cgaatcttac attaaaaggc agcaagtatg
    34801 tattggaaaa taaagaagta gaatatccga catcttatgc aagtaacgaa tttctttcag
    34861 ataccgcaga attttctttt tcagatggta tgattgcagt tattcaaagc aaagattgat
    34921 ccataaacaa ataaaaaaat ttgtctttca ggctaaaaat gacagaatct attatttgct
    34981 aaaaaaaagc ttaagaggag caacttacct tttgtttcac ggcatttagt gataaaattg
    35041 aacacaagag tctaattaat tcgaggtgtc tttgtgaaga agttcaatcc taataaaaat
    35101 attattatta ccttggttat tgttatcatc gtggtgacga tactaagtgt gaccgctgcg
    35161 cagcgagcga atgaaggaaa gcccaatttc ttccaatcca tcgttaatga cagtgttgca
    35221 atggttgata aaacaatctc tgccccagta cggtgggtac gaaatggagt agattctgtc
    35281 cataatcttt tttcaactta tgccgaaaat gagcggctaa aagaaaaaat cgatagctat
    35341 gatgacgttt cacagcaaaa caagaattac gagagagaaa tcgaagcgtt gaaaaaagaa
    35401 cttgagttga atcagacgct gactagttac gaaaaagtaa cggcaaatgt cattactcgt
    35461 tcgcctgaca gttggcagga catgctgatc gtagacaagg gttctaaaga cggtatcgaa
    35521 gcaaatatgg cagtcatgtc gcaaaaagga ttgattggcc gagtgatcga ggtcaatacg
    35581 gcttcgtcta aaatcgaatt actgtcatcc tctaatgaaa gctccaatca ttttccagta
    35641 cgggtatctt cggctaatgg cgaagcgttt ggtttgctta aaaactatga tgaaaagctc
    35701 catgccttag tggtgaccca attaactggt gatacggata tcaaagaagg ggatgttgtc
    35761 cagacatccg gtcttggagg gaattctcca gctaacttgc cgatcggtac ggttattaaa
    35821 acgaaaccag atagttatgg gctggatcgg gaagtttatg tgaaacctta tgcagaaatg
    35881 tatgacgtgt cagttgtgac gattgtccaa cggctagcgg aggattagtc catgttgaag
    35941 aaagaaacaa tgaagtacta tctgccaatc gttttgttct ttttgatgtt gatagatggt
    36001 catttaacaa gaatgctagg ggagtggtcg aaaggcacct atatgtcaaa tgcccacttt
    36061 ctgatattgg cattattatg ttgcagtatg gcgtttgaaa aacgttattt actgattacc
    36121 acgattgttc tcggggctat ctatgacgct tactatattg gcgttatcgg tatctatgca
    36181 gtagctctcc ctttaattgt atggttgatg tatgtaatga aagacgttat ccatgtcaac
    36241 atctttactg aatttttcag tatgatcatc tttgtcacgg gttatgaatt gtttacgatg
    36301 gtggtccagt tgatttttaa attagcagta gtaaataaca cgtattttat tacaaggttt
    36361 ttaggaccta cactgctgtt gaacatgatt atatttgtat tattcatttt tccctttaag
    36421 aaattattca gtgatgaata atttcttttt ttatttttag gtcgaaagcg aaaaagaatc
    36481 gttgttatct taagaaatgc tagacttaac gcggcttttt catgatcatt ttctgaaacg
    36541 aacagttttg tcatttttaa aagttgtttt gaaacatttt tgtaacataa ctattatatc
    36601 gttgacacac tgctatggta caatttttat atcgttaaga aagacttaaa tatatttaga
    36661 tatttattaa attaagccag tcggaggaat gaagagtgaa aaagagttta atatcagcag
    36721 taatggtatg ttcaatgacc ttgactgccg tagcatcacc gatcgctgcc gcggcggacg
    36781 attttgattc tcagatacaa cagcaagatc aaaagattgc agacttgaaa aatcaacaag
    36841 cagatgcaca atcacaaatc gatgccttag aaagccaagt ttctgaaatc aacacacaag
    36901 cacaagacct attagcaaaa caagatacat tgcgtcaaga atctgcacaa ttagtaaaag
    36961 acattgcaga tttacaagaa cgtattgaaa aacgtgaaga tacaatccaa aaacaagcac
    37021 gcgaagcaca agtaagcaac acaagctcaa actacatcga tgctgtattg aatgctgatt
    37081 cattagcaga tgctattgga cgcgttcaag caatgacaac gatggtaaaa gcaaacaacg
    37141 acttgatgga acaacaaaaa caagataaaa aagctgtaga agacaaaaaa gctgaaaacg
    37201 atgctaaact aaaagaatta gctgaaaacc aagctgcttt ggaatctcaa aaaggcgatc
    37261 ttttatctaa acaagctgat ttgaacgtac tgaaaacttc attagctgct gaacaagcaa
    37321 ctgctgaaga caagaaagct gacttgaacc gtcaaaaagc agaagctgaa gcagagcaag
    37381 ctcgtattcg tgaacaacaa cgtctagcag aacaagctcg tcaacaagct gcacaagaaa
    37441 aagctgaaaa agaagcacgc gaacaagcag aggctgaagc acaagctact caagcttcat
    37501 ctactgcaca aagctcagca acagaagaat catcagctac acaatcttca atgactgaag
    37561 aatcatcatc agctacacaa tcttcagcaa cagaagaatc aacaacacct gaatcttcaa
    37621 cagaagaatc aacagcgcct gaatcttcag caacagaaga atcaacaaca gcgcctgaat
    37681 cttcagcaac agaagaatca acaacagtgc ctgaatcttc agcaacagaa gaatcaacaa
    37741 cagtgcctga atcttcaaca acagaagaat caacaacacc tgcgcctaca acaccatcaa
    37801 cagatcaaag tgttgataca ggaaacggca caggaagtag tactccggct ccaacgccaa
    37861 caccaacacc tgaacaacca aaacctgtaa cacctgctcc agctccttct ggaagtgtga
    37921 atggtgcagc aatcgtagca gaagcttaca aatatattgg tactccatat gtttggggcg
    37981 gtaaagatcc aagtggcttt gactgctcag ggttcacacg ctatgtttac atgcaagtaa
    38041 ctggtcgtga catcggtggt tggacagttc ctcaagaatc agctggaaca aaaatttcag
    38101 tttctcaagc aaaagctgga gacttgttat tctgggggtc acaaggcgga acttaccacg
    38161 tagcgattgc tttaggcgga ggacaatata tccatgctcc tcaaccaggc gaaagcgtaa
    38221 aagttggttc agttcaatgg tttgcccctg actttgctgt cagcatgtaa gaatgattaa
    38281 tcaaaaagag ttagcccatt ctggactaac tcttttttta ttgtgataaa atagaaatca
    38341 gataaattct tctgtcaatt ccatgaacgt ttggatatct tcagtcacca tgtcgatacc
    38401 tgcttgccaa aaatctggtt gggtaagatc gacattcaaa tgtttcttcg caagatcttc
    38461 cgtcgtcatt gaggcagtat cgcgtagtaa atggatatat tcttcttcaa aatttgctcc
    38521 cttttggttg gcgtaagcat agatgcccaa actaaagagg tagccaaatg tgtatgggaa
    38581 gttataaaac ggtacgtcat caataaagaa atgcaatttt gcagcccaga aatatggatg
    38641 gtaagtagct aaagctcctt gatagccttc tttttgcgcc tcaaccatca tctctgtgat
    38701 cttatcttct gagactaatc cttcttgccg tgcttcatag aatcgatttt caaaaatgaa
    38761 tcgggaatgg atattcatga acatagctaa agcattttgc agttttgtat ctagcaaatt
    38821 gattttttct tcttttgttt ttgcgacttt taatgtagca tcggctacga tcagttctgc
    38881 gaatgtactt gctgtttctg cgacattcat ggcatattcg cgatttaaag aaggtaaatc
    38941 ccacatcgtg ctgctatgga aagcatgtcc tagttcatga gcaagtgtcg caacctcatt
    39001 aacagaatta ctgtaggtca taaagatcct tgattctttt gtttctggta actcagtaca
    39061 ataaccacca ggacgtttcc caggacgatc ttctgcttcg atccagctct tttcaaaagc
    39121 agattgggca aatttagcca tttttggact gaatttgtta aagttttcga taataaatgc
    39181 tgctgcttcg tcaaaactaa aggttttttc tttcaaatcg cctaaaataa taggtgcatc
    39241 ttgatcttgc cattccattt tttctttacc gaaaagatta gcttttctag tcaagtaatt
    39301 aacaaggggt tgtttgtttt tctggattgt atcccacatg acagacaatg tttctttctt
    39361 caaccgattg tactctaatg gtttttgtag aaaatctgtg acaccatgaa gttcatagtc
    39421 agaaagtcta aaaccatcta aatgattcaa agtatccgtg aagaggggtg ccttttcttt
    39481 ccaggcttct tcccatgctg cgaaaagtgt ttctctgact tttggatctg gatcgcccat
    39541 catcttattg aaggcttgtc cagcagataa ttcgactact tgtccatctt gttcgaaggg
    39601 aatcgaaatg ctggctacaa tcgtatcata atgactgctc caagcattta gaccatctaa
    39661 agaaagcgta tttataatgt tttcttcagc ttctgataat aattgtgagc catcacgacg
    39721 aatctcgttt aaacgaaaag caattgtttc aaacgaagat tgagaaagca tctgattcca
    39781 atcattgtca gatatttctg caaatttctt tgacaataca gtttcggctg attgcagacg
    39841 aggcagcatt gcgtaaagct tgcctgataa gacctttgca tctgaatcat tgacattggc
    39901 agaaagcaat gcagtaatat agctgctgca ttgggtaaaa ccattcgtaa tcgtttcttg
    39961 caattgaaga atagcgttta actgctctgc attgtcagaa ctaggtgacc attttgttac
    40021 gcggtgataa tactcgttca tttggtcttc caattgcttc atccgttggt tcaaagcatc
    40081 agaatgactg cctccggaaa aaatagaatc tagattccaa tttaatgaat aactcatgaa
    40141 aaaactccct tcatattgta atacaaaatt aagtatagca cttttttatg gaagaagtct
    40201 ctatgaaaat gattgttgtt tcttcaaaaa aagtctaaga taaaatacag aggtgactta
    40261 tgaattcttc aaaaaaacaa atgaaaaacg gaaatattta tttagtaatc gcactgatag
    40321 tcgttataat cttattttac agttcttctc aaacttacgg acagcaatca caagtttcgc
    40381 gattagaaag ctggctgcct aatcagccat ttaaagacac attagcgggt atacgtttta
    40441 attatggtgg aagtatggtg agtatcaacc atctgggcta cgccaaattt ttagagttct
    40501 ttttaaggaa gggcgcacat ttcggtacct attttatttt gggaggcagc ttgtttttag
    40561 gtgttttccc caagctaaaa atttggtggc tgacagcgat ccttgcttgg ctttcagcaa
    40621 cagggtacgc aggtttggac gaattccatc agatgatgac cggtggacga acacctatgt
    40681 tccaagatgt catgttggat tcgatcggtg ctctgactgc tgtgatatta tgtgtgcttt
    40741 ttgcggcatt caagaagcgt ttatagattg gctgtggctt tgtttttttg tgaaatagta
    40801 ctagaataga agtgtattct caaaagaaca agggaggaaa aatcatgtca ggacatagta
    40861 aatggcataa tatccaagga agaaaaaatg cccaagatgc aaaacgaggg aaagtctttc
    40921 agaaactttc tcgtgaaatt tatatggcag ctaaagcagg cggtcctgat ccatctatga
    40981 atccagcttt gcggcttgtt gtggacaaag caaaagctgc aaatatgcct aacgacaatg
    41041 tggaacgggc gattaaaaag gctacgagtg ccgtagatga aacgaattac gatgaaatca
    41101 cgtatgaagg atatggtccg ggtggagtag gaatcttagt ccatgcattg acagataacc
    41161 gtaatcggac agcgacaaat gtccgagtag cttttactcg aaacggtgga tcactgggag
    41221 aaacgggttc agtcagttac ttgtttgacc gcaaaggata tatcgctatt gaacgcgatg
    41281 ggttgactgt tgatgaagag acgatgtttg aggatgtatt agaggcgggg gcagaagatc
    41341 tgcaaacttc tcctgaagta ttcgagattt atactgcacc agaagatttt gctgctgtac
    41401 gagatgagtt ggaaaaagag ttcactttgg cgcaagcaga attgacgatg attccacaaa
    41461 cgacgattga tttaaatgag gaacaaaaag agcaattgga acgtctagta gataagctag
    41521 aagacgatga tgatgtttca gaagtcttta ctgctgctga aatgtaagta gcacattgtt
    41581 agataataaa aagaaagcac ctgtaccaat cgtacgggtg ctttcttttt atatattatt
    41641 tttgttagaa gaccaataca ggcgttcctt tttcgaccat cccgaataat tccttcatta
    41701 cacttggtgg ggtgttgatg cagccgtgtg aaccgcgggt tttccataag tctccaccgt
    41761 attcaggttg ccagtctgaa tcgtggatac caacacctgt ccagtcaatc ggcatccagt
    41821 agtttaccgg actctcgtaa ggtgttccat cgtcatttgt tccttttaaa gttgcatctt
    41881 cttctttatt ccaaacatag aagactccag caggtgtagg tgtagttggc tttccggaaa
    41941 cgatatctgt ttcaagggcc acttttccgt ctttgtaata ccacatatgc tggttctcca
    42001 gatcaacttc gatatacgta tcttcgatca atggatgatc agcggttgtg ccaccttgca
    42061 caatgggtga acgagtaaaa tcttggcctg ccagaatcgc ttttttcaat gcttctgtct
    42121 cactgtctgt ttggatcgtc caactgtaag tgccaactgg gaccgtaact tctcctcttt
    42181 ttgtactttt aaattttgtg tcattcgtac ttgtgttata ctttgtgcct aaatctgtta
    42241 catattgacg tacttgttct gtatctaaat cgacttttcc atcattgtag gtcagccaac
    42301 tcataatatc acttgatggg atctggaaag tctcaccgtt tatactgtaa gtagctttta
    42361 catttgcgat agcattcatg gatgcgagtt gttctttcag tgagctgtct tcagatgtta
    42421 cttttggttt ttctttgaat tcagtcaatt caattgtatc tttaccagag tttaccgcac
    42481 tttttaattg tttgaccgca gcatcgacgt cgatcgtatt tccattgact tcaggcttga
    42541 taacgaaaga gtcacctgct ttttcgatcg ttgcatcagt agtaggtgtc cggtctttgt
    42601 tcaaatcagt cagcttagtt gtcaatgtct gaacggtagt gtctagtttt tggtggtttt
    42661 gactgacagg atctatttct tgtttttctg ccgctgagac ataagtcatt ccccaagtcc
    42721 atccgttttg ttcatttagg atcggagaaa gctccgaagt gaaatcatct ttgtagccga
    42781 gctcggattt tggtacttcc tgccagactt ttccatcttc tttaatagcc agttttttat
    42841 cagaataagc ttcttttaat ttttcatttg ctttttcgac tgttagattg ctgacattta
    42901 ttccatttat ttctgtattc ggcaaaaaat gctttgtgta gtatgtactg cgataggtat
    42961 aaaagctgat cagcactaag agaacaccaa gaactgaaag caaagcaaca tgtttcgatt
    43021 tttttcgatg cgaagatctt gtcatatcga caactcctta agtaaaagtt tagggtggaa
    43081 cggtctcatg atagcataat ttagaggaaa agacgaaggt tatctctact ttatgaaaaa
    43141 atttagaaac cataagggtg ataaaataaa aaaacggaaa acaccgaata gatgttttcc
    43201 gtcatgttca aagattattc agcgtctagt ttagcaaaag cttcatctag aatttctttt
    43261 aattgcttag cagaagcagc catacggtct tgctcgctgt cacttaaagg aatttcgatt
    43321 actttttgga taccttgacg gttgataaca gcaggtgcac caatgtagat gtcgttcaat
    43381 ccatattcgc cattcatgaa tactgataaa ggaagaacag cattttcatc atctaagatg
    43441 gcacgagtga tacgtgctaa tgctactgcg ataccataga aagtagcgcc ttttttctcg
    43501 ataattgtgt aagcagcgtc acgtacgcca aagaataggt taaccatagc ttcttcatcg
    43561 atttctgggt tgttttttac ccattcatag atttgcaagc cggcaacatt tgcatgtgac
    43621 caaactggga attctgaatc tccgtgttca cctaagatgt aagcatggac gttacgtgca
    43681 tcaacgtcaa ctaattccgc aagtgcttga cggaaacgag ctgagtcaag agaggtacct
    43741 gaaccgatta cgcgttcttt agggaaacct gagaatttcc aagttgagta agtcaaaata
    43801 tctactgggt tagcggcaac taaaaagata ccgttaaatc ctgattcaac gatttgtgtg
    43861 acaacatcgc ggttgatttt taggtttttg tttactaagt ctaaacgagt ttcacctggt
    43921 ttttgtggtg cgccagctgt aataacaact aagtcagcat catgtgcgtc tgcgtaagta
    43981 gcagaataga tttttttagg tgaagtaaat gccaaagcgt gagacaagtc aattgcgtct
    44041 ccttcagttt tagcagtatt gatatccaca atacctactt cttgagcgat attttgagta
    44101 actagagcaa aggcatagct agaacctacg gcgccgtctc caacaagaat aactttttga
    44161 tgatcttttt tttctgcagc tgcagtcatt tagtatcaat ccttccattg atttgttttt
    44221 tcccatgctt atgctaacat ttctcaagcc ccatgtcacg ctatttggct ataaaatata
    44281 acagataaca aggttgtatt atatgtaaaa gcttacatgt gcgttatttc actattttta
    44341 tatatcaatt atttcattaa actttgaaga aatttttaat ttctttatca tttattaacg
    44401 attctctttt agccgaaacg ccttgttttg tgttatacta tgcattgaca agtgtaaaaa
    44461 agaaaaaagc tcgatggact ggaccgctaa ttagcagtcc agccttttgt gctgcttttt
    44521 agaattaggt ggaaaacaaa aatgaaaatg atcgtcgggt taggaaaccc aggtaccaaa
    44581 taccaatata caaaacacaa tatcggcttt atggtcgtgg ataaaattgc acgcgagcat
    44641 caagctactt tcaaaaaaaa tccgttcgaa gcagaagttg ccgaattttt tcataatgga
    44701 gaaaagatcc ttttagtcaa accacaaaca ttcatgaatg aatctggtcg tgcagtcggc
    44761 ccactaatga cttattttgg gatttatcca gaggaattag tcgtaatcta cgatgatttg
    44821 gatctggcag tgggaaaaat ccggctccgt caaaagggaa gtgccggcgg gcataacggt
    44881 atcaaaagta ttatttccca tttgaatacg aatgtctttg atcgaatcaa ggtgggaatc
    44941 ggcaggccag aagggaaaaa aacagtagtc cagcatgtgc ttagtccttt cagcaaagaa
    45001 aatcagcctt tgatagaaga aagcatgtgt caatctgtta aagcagtcga atatctcata
    45061 gaagggcatt cgttcgtgga tgcgatgaat cgattcaatt gaggaaggag gaaaaggaat
    45121 ggatattatt caattagtcg gaaaagatcc gcaagtaaaa gaatgggtca gcaatttaga
    45181 agaaaaaatg cctagacaac taatcacagg attagcgggc tcagcgaaaa cacttctatt
    45241 tgctcgagcg tttaagaaaa caaataaaaa catgattatt ctgatgccaa atttgtatta
    45301 tgcgaatcag ttagcagaag atctgcaaca tgtgattccg caagatcaga tccatttatt
    45361 tccagtggat gaagtgcttt ctgctgaaat ggccttttct tctccagaag cgcgaggcga
    45421 gagagttgca acgcttaacg cacttcaaga aaaaaaagca ggtatctatt tgttgccagt
    45481 cgcatctttg cacaaacgac tgccgaataa gaaaacttgg ctacaggcgc aattgaattg
    45541 gcatattggt gatgagatag acgtggaaaa tcttcctcgc caattgactt tgatgggcta
    45601 tgtacgagaa gagatggtcg caaaaccggg agaattcagt attcgaggaa gcattatcga
    45661 tatttacccg ttaacgacaa cctatcctgt tcgtgtagaa ctatttgatg tagagataga
    45721 ttctatgcgg tattttgaag cagaaacaca acgctcaatc gaaaaaacgg aagaaatctg
    45781 ggtttcccca acgagtgaac aagtctacgg accagaggat ctagaacatg gaatagagca
    45841 aatgcaaaaa atgctagaaa aacggcttgc tgcgaccaca gaagaagcag accgcgagtt
    45901 tttgcaagat tacttcggtc agctgacttc aagctggcaa gcaggtgtcc cgacagaaga
    45961 agcaaaattc tactcagatc ttctttacca agaatctgtg acgatcttgg attatttccc
    46021 ttcaaatagt ttactagtcg ttgatgatta tcagcgaatc atggaaacaa atcgggaaat
    46081 cgaaagggaa gcggcagaat ggcagactca aaaaatcagc gaattgcggg tgttttctga
    46141 gcagacattt tcagcagata tacaaaaaat cgtgcagaag gaaaagttca ccacaacctt
    46201 tttctcactc ttccaaaaag ggatgggaaa tcttcgtttc caagcaatcc ataattttca
    46261 atatcggacg atgcaacagt tcttcggaca aatgccattg ctgaaaacag aaatggaccg
    46321 ttggcgcaag caaaaacaga ctgttgtgat ctttattccg acaaaggaca gaatacgcaa
    46381 agcagaacaa atgcttcgag atgaagacat attagtagta gaaacagaaa aaaatgcatt
    46441 gatcagagga caggtacaat tagttgaagg agctttgcag gctggttttg aacttcctca
    46501 agaaaaaata gtagctatca cagaaaaaga aattttccaa aaaacgacga aaaaacaaac
    46561 gagacgtcaa acagtaacaa acgcagagcg attgaaaagc tataatgagt taaaagcagg
    46621 agattacgtt gtccatgcca atcatggtat cgggaaatac attgggatgg aaacgctaga
    46681 agtagatggc gttcaccaag actatatgac tattttgtat caaaacgacg acaaactgtt
    46741 tattcctgtt actcaactga atctgatcca aaaatatgtc gcctctgaag ccaaagcgcc
    46801 tcgcatcaat aagctaggcg gaagtgaatg gacgaaaacc aagcgaaaag tctcttcaaa
    46861 aatcgaggat atcgctgacg atctaatcaa attatatgcc gcaagagagt cagaaaaagg
    46921 atatgctttt ggacctgatg acgcctacca aaaagaattc gaaaatgcct tcccctactc
    46981 tgaaactgat gaccagttga gaagtgcagc ggagatcaaa cgggatatgg aaaaagaaaa
    47041 accaatggat cgactactcg taggtgatgt tggttatggg aaaacagaag tagcgcttcg
    47101 agcggccttt aaagcgataa aggaaagcaa acaagtagcc tttttagttc caacaacgat
    47161 cttggcacag cagcactatg aaacgatgct ggaaagattt gaaggtttcc ccgtaaatat
    47221 cgggctatta agtcgtttca gaacaagaaa gcaacaaaaa gaaaccatcg aacaactgcg
    47281 tacaggtcaa gttgatattg ttgtaggaac ccaccgaatt ttgtcaaaag atattgaatt
    47341 tagcgatttg ggtctactga tcattgacga agaacaacgt tttggtgtaa aacataagga
    47401 acgattgaaa cagttacgtg cacaagtgga tgttttgact ctgactgcaa caccgattcc
    47461 gagaactttg catatgtcga tgttaggcgt gcgggatctt tcagtaatcg aaacaccgcc
    47521 tgcgaatcgt tatccgatcc aaacgtatgt tatggaaaaa aatcctgggg ctattcgcga
    47581 agcaatccat cgagaaatgg gacgaggtgg acaagtattc tatttatata atcgggtaga
    47641 aacgatcgaa caaaaagtag aagaaatcca agaactcgtt ccggaagcac ggatcggtta
    47701 tgctcatggg caaatgacag aggcgcaatt agaaaatacg ctttttgaat ttattgaagg
    47761 tcaatatgat gttcttgtca ctacaaccat cattgaaaca ggagtagata tcccgaacgc
    47821 aaatacgttg ttcgttgaga acgctgatta tatgggatta tccacgttat accaattgcg
    47881 agggcgggta ggaagaagca gccgtgtcgc atatgcttac tttatgtatg agcagcagaa
    47941 gatccttaac gaagtcagtg aaaaacgtct tcaggcaatc aaagatttta cagaactggg
    48001 atctggattc aagattgcga tgcgggatct ttccattcgc ggtgcaggta atctcttagg
    48061 tgctcagcag catggattta ttgatgcagt cggtttcgat atgtattctc aaatgctaag
    48121 cgaagctgtt tctcgtaaac aaggaaagaa tagtcaagtg gagaagacca ctgttgagat
    48181 cgacctcggt gtcgatgcgt atctacctga aacatacgtg gcagatcaac ggcagaaaat
    48241 cgagatctat aaacgaattc gtgaacttga ttcgcaagaa atgctagatg aactagaaga
    48301 tgatctgctc gaccgttttg gagaatatcc agaagaagta gcacatctct tgacaattgg
    48361 gcagatcaaa atggatggcg atcgagcgct cctagaaacg ataaggaaac aggaggcgca
    48421 tatagtcttt actctaagta aagttggaac aaaacgctat agtgtggaac agttattcga
    48481 agcactgaca gcgacaaaac taaaagccag tctaggcgtt gaaaaagaac agatggtgat
    48541 acgcttgacg atccccaacc aaatggaaga agcagtatgg cttcaggaaa tccaaaagtt
    48601 tgtcaaagcg ttgcgtgagc aaaaatatct gtcagcgtag gaaggagcaa tgatttggat
    48661 cataaagaaa tgcggcgcgt catgcaagga gcatttgtat tgacgattgc ttctttcata
    48721 gcaaaagtat taagtgccct ttaccgaatt cccctccaaa acttagtcgg cgacgaaggt
    48781 ttttatgttt atcagcaagt ttatccaatt tatgggatag caatgacttt agctttatcc
    48841 ggtcttccgc agttcatatc aaagtacgtt gcagaaagaa agaatccttt cgagcgaaag
    48901 caagctttgc aaaatcttta tcccttagta ttttggacag gtgtcttttt gtggttgttt
    48961 gtctttgcat ttagcagctg gattgcttgg atgatgggag acatacaatt gaaaccgctg
    49021 atccaagtag tttcctttac ctttttgctg atgcctggtc tatcttttta tcgagggaat
    49081 ttccaaggaa gattcttgat ggaaccaact gctgtttctc aagtagttga gcagttcgtt
    49141 cgtgtttttg tgattgtctg tagtgcgttt gcttttagaa agcttggatg gacggtgtac
    49201 caaacaggga cggcagcaat gagtggagct gtttttggcg gtttatgcgc atatgggatc
    49261 cttcactact atgaacaaaa gattcatgga ggagcgttaa gcttccgacg gtttccattg
    49321 ttcaaacagc cgtcaaagcc cttgatccgg cgttttctgg tagaaggcgg actggtttcg
    49381 atttatagtg gattgcttat ctttttccag ctgatcgact ctttctttgt aaaaaatgct
    49441 ttggaagttt acggactgtc agaatacggt gcgaagatag caaaaggggt ctatgatcga
    49501 ggtcagcctt tagtacaatt aggtttagtg attgccactg cattaagtgc gacattcctg
    49561 cctgcgttga ctcgtcattt gaccaaccgg atctatcgtc agtttttaca gacagccaaa
    49621 atctatctta gattgacgac cgctttagca ttagctgcgt ctttgggatt agccttgctg
    49681 ctcccttata tcaattatgc gttgtttaaa gattatgcgg gaaatgcagc gcttgtatta
    49741 tttgtttttt ccattgcttt cactgcagtc atccaagctt atcaaagtat tgctcaaagc
    49801 aagaactcgt ttcgcccttc tttaaaaggc gcaggatggg gactattggt aaaaggattg
    49861 actacttctt ttctgaccgg tttattagga acagcaggtg caagcctgtc taccctttta
    49921 ggattaggtg tgacgctttg gtattttgtt cgaattgaaa caaaagagct aaatgctttt
    49981 tggaaagaac ggcattttgg acggaagttg atgttcagcc ttctttgtat gacagtagta
    50041 ctgctgctgt attatggtgt attgaatata gtatttggtc ctgtaactca tcgttccact
    50101 gctttttttg tcagcgtgat aggagttggg atcggggctt ctgtgttcat ttatgcagtg
    50161 attcgtcttc gtctgtttac agtacgagaa tggctaatgt tgccttttgg taagaaaatc
    50221 ctgcgattaa ataaaaaaat ta
//
LOCUS       DASJPC010000008        49183 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJPC010000008 DASJPC010000000
VERSION     DASJPC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.008"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            123..935
                     /locus_tag="U4A13_000460"
     CDS             123..935
                     /locus_tag="U4A13_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN1778788.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            932..1732
                     /locus_tag="U4A13_000461"
     CDS             932..1732
                     /locus_tag="U4A13_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN1778789.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1893..3161
                     /gene="rseP"
                     /locus_tag="U4A13_000462"
     CDS             1893..3161
                     /gene="rseP"
                     /locus_tag="U4A13_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN1778790.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3229..4938
                     /locus_tag="U4A13_000463"
     CDS             3229..4938
                     /locus_tag="U4A13_000463"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN1778791.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5150..9508
                     /locus_tag="U4A13_000464"
     CDS             5150..9508
                     /locus_tag="U4A13_000464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN1778792.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9653..10126
                     /gene="rimP"
                     /locus_tag="U4A13_000465"
     CDS             9653..10126
                     /gene="rimP"
                     /locus_tag="U4A13_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN1778793.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10149..11324
                     /gene="nusA"
                     /locus_tag="U4A13_000466"
     CDS             10149..11324
                     /gene="nusA"
                     /locus_tag="U4A13_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN1778794.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11346..11639
                     /locus_tag="U4A13_000467"
     CDS             11346..11639
                     /locus_tag="U4A13_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN1778795.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11636..11947
                     /locus_tag="U4A13_000468"
     CDS             11636..11947
                     /locus_tag="U4A13_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN1778796.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11960..14266
                     /gene="infB"
                     /locus_tag="U4A13_000469"
     CDS             11960..14266
                     /gene="infB"
                     /locus_tag="U4A13_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN1778797.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14293..14640
                     /gene="rbfA"
                     /locus_tag="U4A13_000470"
     CDS             14293..14640
                     /gene="rbfA"
                     /locus_tag="U4A13_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN1778798.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14797..14970)
                     /locus_tag="U4A13_000471"
     CDS             complement(14797..14970)
                     /locus_tag="U4A13_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1778799.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15418..17780
                     /locus_tag="U4A13_000472"
                     /pseudo
     CDS             15418..17780
                     /locus_tag="U4A13_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            18148..19070
                     /gene="truB"
                     /locus_tag="U4A13_000473"
                     /pseudo
     CDS             18148..19070
                     /gene="truB"
                     /locus_tag="U4A13_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            19074..20015
                     /gene="ribF"
                     /locus_tag="U4A13_000474"
     CDS             19074..20015
                     /gene="ribF"
                     /locus_tag="U4A13_000474"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN1778800.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20367..21152
                     /locus_tag="U4A13_000475"
     CDS             20367..21152
                     /locus_tag="U4A13_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1778801.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21165..22016
                     /locus_tag="U4A13_000476"
     CDS             21165..22016
                     /locus_tag="U4A13_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN1778802.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(22140..22592)
                     /locus_tag="U4A13_000477"
     CDS             complement(22140..22592)
                     /locus_tag="U4A13_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778803.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(23057..23425)
                     /locus_tag="U4A13_000478"
     CDS             complement(23057..23425)
                     /locus_tag="U4A13_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN1778804.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23575..24057
                     /locus_tag="U4A13_000479"
     CDS             23575..24057
                     /locus_tag="U4A13_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN1778805.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            24094..25104
                     /locus_tag="U4A13_000480"
     CDS             24094..25104
                     /locus_tag="U4A13_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN1778806.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25219..25932)
                     /locus_tag="U4A13_000481"
     CDS             complement(25219..25932)
                     /locus_tag="U4A13_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778807.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(26122..26712)
                     /locus_tag="U4A13_000482"
     CDS             complement(26122..26712)
                     /locus_tag="U4A13_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN1778808.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27196..27423
                     /locus_tag="U4A13_000483"
     CDS             27196..27423
                     /locus_tag="U4A13_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN1778809.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27439..28605
                     /locus_tag="U4A13_000484"
     CDS             27439..28605
                     /locus_tag="U4A13_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEN1778810.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28839..29882
                     /gene="hrcA"
                     /locus_tag="U4A13_000485"
     CDS             28839..29882
                     /gene="hrcA"
                     /locus_tag="U4A13_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN1778811.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29976..30539
                     /gene="grpE"
                     /locus_tag="U4A13_000486"
     CDS             29976..30539
                     /gene="grpE"
                     /locus_tag="U4A13_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN1778812.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30590..32419
                     /gene="dnaK"
                     /locus_tag="U4A13_000487"
     CDS             30590..32419
                     /gene="dnaK"
                     /locus_tag="U4A13_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN1778813.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32570..33736
                     /gene="dnaJ"
                     /locus_tag="U4A13_000488"
     CDS             32570..33736
                     /gene="dnaJ"
                     /locus_tag="U4A13_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN1778814.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33818..34483)
                     /locus_tag="U4A13_000489"
     CDS             complement(33818..34483)
                     /locus_tag="U4A13_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1778815.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34459..34632
                     /locus_tag="U4A13_000490"
     CDS             34459..34632
                     /locus_tag="U4A13_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778816.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34677..35414)
                     /locus_tag="U4A13_000491"
     CDS             complement(34677..35414)
                     /locus_tag="U4A13_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1778817.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35416..36918)
                     /locus_tag="U4A13_000492"
     CDS             complement(35416..36918)
                     /locus_tag="U4A13_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN1778818.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37177..39171
                     /gene="uvrB"
                     /locus_tag="U4A13_000493"
     CDS             37177..39171
                     /gene="uvrB"
                     /locus_tag="U4A13_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN1778819.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39181..41997
                     /gene="uvrA"
                     /locus_tag="U4A13_000494"
     CDS             39181..41997
                     /gene="uvrA"
                     /locus_tag="U4A13_000494"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN1778820.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42260..43525
                     /locus_tag="U4A13_000495"
     CDS             42260..43525
                     /locus_tag="U4A13_000495"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN1778821.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43529..44260
                     /locus_tag="U4A13_000496"
     CDS             43529..44260
                     /locus_tag="U4A13_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN1778822.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44395..45279
                     /gene="rapZ"
                     /locus_tag="U4A13_000497"
     CDS             44395..45279
                     /gene="rapZ"
                     /locus_tag="U4A13_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN1778823.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45276..46274
                     /locus_tag="U4A13_000498"
     CDS             45276..46274
                     /locus_tag="U4A13_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN1778824.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46308..47243
                     /gene="whiA"
                     /locus_tag="U4A13_000499"
     CDS             46308..47243
                     /gene="whiA"
                     /locus_tag="U4A13_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN1778825.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47786..48436
                     /locus_tag="U4A13_000500"
     CDS             47786..48436
                     /locus_tag="U4A13_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778826.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48623..>49183
                     /locus_tag="U4A13_000501"
     CDS             48623..>49183
                     /locus_tag="U4A13_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEN1778827.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        16465 a         8421 c        10575 g        13722 t
ORIGIN      
        1 ttaaaaaaag aaaaaattat tcagttttta aataaaaaaa caatgatttc ataggaaaaa
       61 cagttagata ttgctataat acttaagata gtgggaatct tttaagtata ggaggaaaaa
      121 gaatgttacg tttttttccg caaaaaaaca aatatattca agaaaaaagc gaatttcaat
      181 ttaataccga tggaccaatt cctcagcaca ttgccatcat catggacggc aatggtcgtt
      241 gggcgcaaaa ccgtcgtttg ccacgagttg ctggccataa agaaggcatg gaaacagtaa
      301 agaaagtcac aaaaaaagca tcaaggcttg gagtgaaagt gttgacgctt tatgcctttt
      361 cgactgaaaa ctggaagcga ccaaaagatg aagtaagttt tctcatgcaa ctacctgtcg
      421 acttctttga tacgttcgta ccagaattga ttaaagaaaa tgtcaaagtt catgtcatgg
      481 gttatgaaaa cgtattgcca gaacatacgc aagatgctgt aagacgtgca attgagcaaa
      541 caaaaaacaa tactggaatg gtattgaatt ttgctttgaa ttatggaagc agagcagaga
      601 tcgtgactgc tgtccaagaa attgccgaag aagtagcaaa aggagaaata cacgcagaag
      661 aaatcgatga tgaattgatt gctaagcatt taatgacagg tttcttgcct aaagagttac
      721 aagatcctga gctgatgatc cgtacaagtg gagaagagcg aattagtaat tttctgttat
      781 ggcagatcgc atatagtgag ctgtatttta caaaagcgct ttggcctgat tttgatggtg
      841 cgcatcttga agaagcaatc gcctcttatc aaaatcgaga ccgtcgattc ggcggtgtaa
      901 aaaaaacaga agatgagaaa ggggatcagt catgaaacaa cgtgtggtca cagcagccgt
      961 ggcgctagtc ttgtttattc cgattatatg gatcggaggc atagcggttg agcttacagc
     1021 agcaatctta gcagtaatag gagtttacga gctgtttcgg atgaaaggat tgacactctt
     1081 aagttttgaa ggagttttat ccgccgttgg tgcagtgtta cttgttttgc caaaagaaag
     1141 atggtttttc tttcttccag agaatgtaag tacatttatg ttattttatc ttatagttat
     1201 gattctttta ggaacatcag ttatttctaa aaatacgtat acgattgatg aagcaggttt
     1261 tcctgtgctt gtcagcttgt atgtgggaat aggtttccag aattttgtca atgcacgaac
     1321 agaaagcttg gctgttttga tatttggtct atttattgtt tgggcaactg atatcggagc
     1381 atatatggtt ggtcgacgtt atggtcagca taagttaagt cctgatattt cgcccaacaa
     1441 aacgattgaa ggtgcgctcg gtggtattgg tagtgctgtt gtagtcgcat ttctttattt
     1501 attgctatac ccagcaaaag aactatttaa tcatggtatg tttgtcatgc ttctgatgac
     1561 gattattttt tcagcagtcg gacaatttgg tgatttggtc gaatctgcaa tcaaacgtca
     1621 ttatgatgtg aaagactcgg gaaatatctt accaggccat ggtggaatct tagaccgttt
     1681 cgacagtttg ctgtttgtat ttcctatcat gcatttattt ggcattattt agtaagtata
     1741 cctgcgtttc tttgtgaatt tcataaaaga cgcaggtttt tatttttcat tatattgata
     1801 ctattggtgt ctttggtata ctatggtaaa attatagtgt tttatcgttg ttttataaaa
     1861 aacacaaaaa acaagtaaag gagaaaaaag ctatgaaaac gattctgaca tttatcatcg
     1921 tttttggtat attagtgatt gttcatgagt ttggtcattt cttctttgcg aaacgatcag
     1981 gaatcctcgt tcgcgaattt gctatcggta tgggtccgaa aatttatggg catcaagcaa
     2041 aagacgggac aacatatacg cttcgcctat tacctattgg cggttatgtt cgaatggcag
     2101 gcaatgggga tgatgaaaca gaaatggcac caggaatgcc tctttctctt ttactaaatt
     2161 ctgacggcat cgtagagaag ataaatttaa gtaagaaaat ccaattgaca aatgctatcc
     2221 cgatggaatt gagccgttat gatttagaag atgagctgac tatcactggt tatgtgaatg
     2281 gagacgaaac agaagtagta acctaccctg ttgatcacga tgcgacgatt attgaaaacg
     2341 acggaacaga aatcagaatt gcgccaaaag acgtacagtt ccaatcggct aaattgtggc
     2401 agcgtatgct gaccaacttt gctggaccca tgaataactt tattttggca atcctgttat
     2461 tcatcattct ggcatttatg caaggtggtg ttcaagtgac gaacactaat cgcgtaggag
     2521 agatcatgcc taatggagct gcggcagaag caggcttaaa agaaaatgat gaggtagtca
     2581 gtgtggacgg aaaagaaatc cattcatgga atgatctgac aacagtcatt acgaaaaatc
     2641 cggataaaac attagacttt aagatcgaac gagaaggtca ggtgcaatct gtcgacgtga
     2701 caccgaaatc tgtagagtca aatggcgaaa aagttggcca acttggtata aaagccccaa
     2761 tgaatacagg ttttatggat aagatcattg gagggacgcg tcaagctttc agcggttcat
     2821 tggaaatttt caaagcctta ggatcactat ttacaggttt cagtttagat aaattaggcg
     2881 gacctgtcat gatgtaccaa ttgtcttcag aagcagcaaa tcaaggtgta acaactgtta
     2941 ttagtttgat ggcgcttctt tcaatgaatc tcggaatcgt caatctgctt ccgattcctg
     3001 ccttagatgg cgggaaatta gtcttgaata tttttgaagg gattcgcggg aaaccattaa
     3061 gtcaagaaaa agaaggaata ttgacattag ctggctttgg atttttaatg ttgttgatgg
     3121 tgctagttac atggaacgat attcaacgat tctttttcta gaaaaagttg acaaataggg
     3181 tataataaca aacgataact attttgagga aataaaggag tatatagaat gagacaatca
     3241 aaaatgttga ttccaacatt acgcgaagtt ccaaatgatg cagaagtatt aagccatcaa
     3301 atcttgctaa gagctggata tatccgccaa gtatcagcgg gtatttattc atatctgcca
     3361 ttagcaaatc gtgtattgga aaaactgaaa cgcattatgc gggaagaatt tgaaaaaatc
     3421 ggagcggtag aaatgctgat gcctgccatc ttgccagcag aactttggga agaatccggg
     3481 cgttacgaaa catatggacc aaacttgtat cgtttcaaag accgcaatga tcgaaaaatg
     3541 atccttggac caactcacga agaaactttt actgatttga tgcgtaatga aatcaattca
     3601 tataagaaat tacctttaaa tctttatcaa atccaaccta aatatcgtga cgaaaaacga
     3661 cctcgcttcg gtttgcttcg cggaagagag tttatcatga aagatgcgta ttctttccat
     3721 gcaacggaag aaagtttgga cgagacatat aaagattacg aaaaagccta caccgaagta
     3781 ttcaagcgtt gtggcttaga atttcgctcg attatcggag acggtggcgc aatgggcggg
     3841 aaagactcaa aagaattcat ggcgatctca gaaatcgggg aggatacgat ctgttattcg
     3901 actgaaagtg attatgctgc aaacttggag atggctacaa gctactatgt atcaaaaaaa
     3961 tcgcatgaaa cacagcttga attggagaaa gtagctacac ctaacgtaaa atcaattgac
     4021 gaagtagcag atttcttttc tgttgaacct caaaaaatca tcaagtctgt attgttcatt
     4081 gcggatgaac aacctgtcct tgttttggta cgcggagacc atgaagtcaa tgacgtaaaa
     4141 ctgaaaaact ttttatctgc tgacttttta gaagaagcaa cagaagaaga agcacaaaaa
     4201 tatctaggtg ctgagtttgg ttctgtcgga ccagtcggtg tttcagatga cgtaaaagta
     4261 tatgcagacc gttatgtcca agatttagcg aatgccatta ctggagcgaa cgaaacagga
     4321 ttccactata aaaatgtgaa tccagaacgc gatttcactg tactttctta tgaagatctt
     4381 cgttttgtac aagaaggaga tccttcacca gacaacaatg gtgttcttgc ctttactcga
     4441 ggaatcgaaa tcggacatat cttcaaaatc ggcactcgtt acagtgaatc tatgggagca
     4501 actgtactag acgaaaatgg acgagaaaaa tttgtgatta tgggctgtta tggtatcggt
     4561 gtaagtcgtt tattatcagc aatcgtagaa caacattcag atgaacaagg aatcaactgg
     4621 ccaaaaggaa ttgcgccatt tgatttgcat atcgtccaaa tgaatctaaa agacgaatac
     4681 caaacaaacc tgacgaatga actagaagaa gcgatgacaa aagctggcta tgaagtactg
     4741 gttgatgaca gaaatgaacg cgcaggagtg aaattcgcgg attctgattt gattggctgt
     4801 ccaatcagaa tcactgtcgg taaaaaagca gtggatggaa tcgtagaagt gaaaatcaag
     4861 aaaactggcg aaatggttga agtacgtaaa gaagaattgg tgaatacttt accaatcctc
     4921 ttgaaccaag gcgaataata agcgttaaaa cacagaaaat aaaaatagct gaatcagctg
     4981 tttttgacaa tctgatgatt cgcttttaaa attggaactg cgatttgaat cgcagttcca
     5041 attttttaat gtaaaactca gaaaaaacga gtatactaaa aggtactgaa aaagaagagg
     5101 ataagatgcg atcaattgca ttgtttctca tcagaaaggg cgtatatctt tgtcagaaaa
     5161 acgagattta tttgaaaaac tgttggaaca aatccaacta gaggaagcag aaaaaaatca
     5221 tccactgctt actgctggtg aaatggatag tgtagtggtc catcgtaaat cgcgtttatg
     5281 ggaatttacg ctgcattttt ccaaaatttt gcctattatg ctatatcggt cattgactca
     5341 gcatctgact atcgctttta aagatattgc acaagtcaaa ctgaatatct tagcagatga
     5401 ccagacattc gatgaacagc tcttgcaaga ttactgggcc caagctttgg aaaatcagca
     5461 atgcgataca ccactagcac agcaagtatt gaaaacgcag gtacctgtga taaaagatcg
     5521 taaagtcatc ttgccaatcg acagtacagg agctatttct tatttgaagc aacaatactt
     5581 gccattagta gaagagttgt tcgtcagtta cggattccca aaatttcgta tcgaaccaga
     5641 agtggatgag cagcaagcgg aacgagtatt gaaacttttt gaagaacgca aacaagaaca
     5701 ggcagaagcc tttatgaagc aagcagctga aagtctagtt gtccacgaac aaaagaaaaa
     5761 ggaaagaaaa gagcaatcgc cagctcttga aggacccatc cacatccagc taggacgaaa
     5821 tattccagcc gatgaaccaa cgacaccaat gatcagtatc gttgaagaag aacgaagagt
     5881 cacacttgag gggtacgtat tcgacaaaga agtcagagaa ctacgttcaa aacgcaagat
     5941 cttgactttg aaaatcacag attatacttc atcatttatc gtcaaaaaat tctcgaacgg
     6001 cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt agctggttaa aggtacgagg
     6061 aagtattcaa gaagatacgt ttgtccgtga tttggtcatg aatgcgcaag atatcataga
     6121 agtcaaacat acacctcgca aagattatgc tcctgaaggg gaaaaacgag tagagcttca
     6181 tgttcatagc aatatgagta cgatggatgc aacaaacagc atctctgatc ttgtggcaca
     6241 agcagggaaa tggggacacc gagctatcgc cattacagac cacggtggtg cgcaagcctt
     6301 tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa atcttatacg gcgtggaagc
     6361 gaatgtcgta gatgatggtg tcccaattgc ttataatgat gcacatgaag cattgagtga
     6421 agccacatat gtcgtatttg acgtggaaac gacaggattg tctgcagtat atgacacgat
     6481 catcgagcta gccgcagtca aaatgtataa aggaaatgtg atcgaaagct ttgatgaatt
     6541 tatcgatcca ggacatcctt tgtcacgaac aacggtagat ctgactggta tcacggatgg
     6601 aatggttcga ggatcaaagt cggaagaaga agtattgcgg atgttccttg aattttcaaa
     6661 agatacaatt ttggtagccc acaacgcggc ttttgatatg gggttcttga atactagcta
     6721 cgcaaggtat ggtattcctg aagcggccaa tccagtcatt gatacgttgg aattagctag
     6781 atatctgtat cctcaattca aacgtttcgg attaggtgtg ttatctaaaa aatttggtgt
     6841 cagcttggaa cagcatcacc gagcaatcta tgatgctgaa gcaactggtc acttagcatg
     6901 gatctttgtg aaagaggcaa tggacaatca taacatgctt tatcatgatc agctaaatga
     6961 gcatatcgga gaaggagatt cttacaaaag ggctcgacct ttccatgtaa cgattttagc
     7021 aaaaaatcag gcaggactga aagatttgtt caaactgatt tcaatgtcga atgtcgaata
     7081 cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa aagatgcgtg aaaatctatt
     7141 gatcggctca gcttgtgata agggagaaat attcgaagcc atgatgcaaa aaggggtaga
     7201 agaggcaaga aaccgagcaa aattctacga ttatattgaa gttatgccaa aagcggtcta
     7261 tgcgcctttg atcgagcaag aattggtaaa aaatgaacat gatttggaag aaattatcca
     7321 aaatttggta gaaatcggaa aaagtttgga taaaatcgtt gttgcaacag gtaatgtaca
     7381 ttatctgaac gaagaagatg ccatttaccg aaaaattttg atcaattcta tgggcggagc
     7441 gaatccgttg aatcgtcata gtttgccgga tgtccatttc cgaacaaccg atgagatgct
     7501 aactgcattt cactttttag gggaagaaac agcgaaagag atcgtagtag aaaatacgaa
     7561 taaaatcgca gatatctgcg aagaagtgat tccagtcaaa gatgaacttt atacaccgaa
     7621 gattccaggt tcagaagatg aaatatcaga attgagttac acaaaagcaa aacaaatgta
     7681 cggagatccg ttacctgaga tcattcaaaa acgattaaaa aaagagttga attctattaa
     7741 tggtaatggc ttctcggtta tttatctgat cgcccaaaaa ctagtgcata aaagtaatga
     7801 agatggctat ctagtaggtt cacgtggctc tgttggctct agctttgttg cgacgatgac
     7861 aggaatcact gaagttaatc cgttagcccc tcattattat tgtccagagt gccaatattc
     7921 cgagtttttt gaagatggta cgtatggttc tggatttgac atgccagaaa aacaatgtcc
     7981 aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt ccatttgaga catttttagg
     8041 attccatgga gacaaagtgc ccgatattga tttgaacttc tcaggtgatt atcaagcaga
     8101 agcccataac tatacgaaag tattgtttgg cgaagactat gtttaccgtg ccggaacgat
     8161 tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa ggatacgaaa gggacaataa
     8221 tctgcagttt cgaagtgcag aagtcgacag attagcaaaa ggcgctactg gagtcaaacg
     8281 gacaaccggc cagcatccag gggggatcat cgttattccc gattatatgg atgtctatga
     8341 ttttacgccg atccaatatc cagcagacga tcaaaattcg gaatggaaaa cgactcattt
     8401 tgattttcac tcgattcacg ataatgtatt gaaattagat attctagggc atgatgaccc
     8461 aactgttatt cggatgcttc aagatttatc agggatcgat ccacaaacga tccctacgga
     8521 tgacccggaa gtgatgcgga tatttgccgg tcctgaagta cttggagtaa gtcaggaaca
     8581 aatttattcc aaaacaggga cattaggtat tccagaattc ggcactcgtt ttgttcgagg
     8641 gatgctagaa gaaacgcatc ctacaacttt tgcagaacta ctgcaaattt ccggactatc
     8701 tcatggtaca gacgtatggt taggaaatgc ggaagaattg attcgacgag gagatgcgac
     8761 actggcggaa gtaatcggct gtcgtgatga tattatggtt tatttgatcc atgctggatt
     8821 agatagcggg atggcattta aaatcatgga aactgttcgt aaaggacaat ggaacaagat
     8881 ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa gaaaataatg tgcccgactg
     8941 gtatatcgat tcttgttcaa aaatcaaata tatgtttccg aaagcccatg ctgcagctta
     9001 cgtattgatg gctcttcgtg tcgcttattt caaggtttat ttccctattt tgtattactg
     9061 tgcatacttt tctgtacgtg cagacgactt tgatctagtc tccatgtgca aaggaaaaga
     9121 tgctgtgaaa caagcaatga aagaaatcac ggacaaagga ttagatgctt ccgtaaaaga
     9181 aaagaatcaa ctgacagtgc tggagttagc caatgaaatg ctggaacgag gattcaaatt
     9241 tggaatgatt gatttgtata aatcggatgc cgttaacttt gttattgaag gtgatacgtt
     9301 gatcgcacca tttagagctg tcccaagttt gggaaccaac gtggcaaaac aaatcgttga
     9361 agcaagaaaa gacggtccat ttttatccaa agaagatctt gccacccgcg gtaaagtttc
     9421 aaaaacattg atcgaatata tgaacgacaa tggcgtattg aaagatctac cagatgaaaa
     9481 tcaattatcg ttattcgata tgctataagc atctgttttc ctgtctgctt gtaattttaa
     9541 gcagagtatg gtatagttct tataggtaat gatactaact gtgagtgagc ggatttttcg
     9601 ctcactcttt ttaatggaat tacgcactta attttttaag gaggcgaaac ttttgagtag
     9661 cgtcgttgaa acagtcacag aaatggtgac accgatttta gatgaacaaa attttgaact
     9721 tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc ttacgagtat ttattgacaa
     9781 agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt gagaaactga gtgaaaaact
     9841 tgatgcagca gatccagatc cgattcctca agcttatttc ctggaggtat cctccccggg
     9901 agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag gcacttggtg attatgtcca
     9961 tgtctcctta taccaaccag tagatggcga aaaacaatat gaaggatttc ttcaatcatt
    10021 tgatgctgaa cagcttacat tgaaaatacg cataaaaaca cgagaaaaag aattgacttt
    10081 tgaccgaaaa aatattgcaa aagctcgctt agctatccaa ttttaatcac ggagaggaat
    10141 cagaaaaaat gagtaaagaa atgttaaatg cgttagacgc tttagaagct gaaaaaggga
    10201 tctcaaaaga aatcgtgatc gatgctttag aagcagcatt ggtttctgca tacaaacgtc
    10261 actatggcca agcacaaaac gtcgaagtgg aatttgatca gaaaaaagga aagatccatg
    10321 tctatgcggt aaaagaagta acggaagaag tgatggattc acagttggaa gtctcattga
    10381 aagatgcact tttaatcaat ccagcatatg agatcggtga tacgatccgt tttgaagtaa
    10441 caccaaaaga ttttgggcgg atcgctgcgc aaacggctaa acaagttatt ttacagcgtg
    10501 tacgtgaagc agaaagaacg attatttata acgaattcag tgcttatgaa aaagacatca
    10561 tgcaaggaat cgtcgaacgc caggataaac gttatattta tgtcaatctt ggtaaaattg
    10621 aggcagttct ttctaaacaa gatcaaatgc caaatgaatt ttatcaacca catgatcgaa
    10681 tcaaagttta tgtttcccga gtagaaaata cgtctaaagg tccgcaagta tttgttagcc
    10741 gcagccatcc agacctattg cgccgcttat ttgagcaaga agtaccagaa gtctatgatg
    10801 gaatcgttga aattgtcagc atcgcccgtg aagctggcga tcgttcaaaa gtggcagttc
    10861 gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt cgggccaaag ggacaacgag
    10921 ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga tatcgtcgaa tggaatgaag
    10981 atccagctgt attcattgca aatgcattga atcctgcaca agtcatggat gtgatcttcg
    11041 atgagacaaa tccaaaggcc tgcacagttg tcgttccaga ctaccaatta tcgctagcaa
    11101 tcggtaaacg cggacaaaac gcacgtttag ctgctaaatt gactaaccat aaaatcgata
    11161 tcaaatcaga atctgacatg acggaatttt atgaaaatca agaacaacaa aaagaaacag
    11221 aagaactgca tgatgaagca atcgttcagt cagatttaac agacgatgaa tatgaaacaa
    11281 ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc ttaactttag taaggaggca
    11341 aagagatgaa acaaaggaaa attcctttac gcaaatccgt ggtttcaggt gaaatgaaac
    11401 ctaaaaaaga gatggtccga atcacccgtt caaaagaagg ggaagtatcg atcgatccaa
    11461 ctggaaaaat gcctggacga ggagcgtatg tctcacttga gccggaagaa gtacaacaag
    11521 cttgggataa aaaaattctc gatcgagtac tcgaaacaac attaacagat gagttttatc
    11581 aagaacttct tgactatgtc actcaccaaa aagcacgtaa agagttattt ggaaaatgaa
    11641 tacagttaat cggcaaaaag ccatgaatct catcggtcta gcgatgcgcg cgggaaaact
    11701 aatcacgggt gaagaactga ccattgctga aatccgcaaa caaaaagcaa aattggtgtt
    11761 tgtggcaacc gatgcaagcg agaatacaag aaaaaagata aaagataaga gttcatatta
    11821 tgaagttcct tgctttgagc tattttctga agcagaaatc acgcagatga tcggaaaacc
    11881 tcgcaaggta ttcggaataa tggatgccgg atttgcgaag aagaccaagg agctaattga
    11941 aggttaggaa ggtgattgca tgggcaacaa aagaatctat gaattagcaa aagaactcaa
    12001 aaaatctagt aaagacgtcg tggaaaaagc acaacaactt ggcatcgatg tgaaaaacca
    12061 tatgggtgcg attactagca atgatgagaa gaaattgcaa caagcgttca aaaacccaag
    12121 cacgaaacaa caagctgcac aacaagcaag cagaaaacca gccaatcaac agccgaacga
    12181 tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat aaaaataacc gcagttatca
    12241 agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa aaaccaacaa caaaccaaaa
    12301 taatacggga aataccagca gccaaaatcg tcaaggaacg agccaaggcg gcaatcaaaa
    12361 ccgtactaac aataacaaca aaccaaacaa taacaatcgc ggcaaattta ataataataa
    12421 ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag caacaatcaa ctaaaccagc
    12481 tgtaccacca cgcaaattcc gtgaattgcc agatgtatta gagtatactg aaggtatgaa
    12541 cgtagcagat attgcgaaaa aaattcatcg tgagccagca gaaatcatta aaaaattatt
    12601 tatgcttggt gtcatggtca atcaaaacca agcactggat aaagatacga ttgaactgtt
    12661 agcgacggac tatggcatgg aaccacaaga aaaagtccaa gttgacattg ctgatatcga
    12721 taaattcttc gaggcagaag aagtcgatcc agataaatta gtctcacgtc cgccagtcgt
    12781 aacgattatg ggacacgtag accatgggaa aacaacttta ttggatacat tacgccattc
    12841 acgtgtaaca agcggcgaag ccggcggaat cacacagcat atcggtgcct accagatcga
    12901 tattgatggt aaaccaatca ctttcttgga tacaccagga catgcggcct ttacaagtat
    12961 gcgtgcacgt ggagcaagta tcacagatat cacgatctta gttgttgctg cagatgatgg
    13021 cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa gctgcaggcg ttccaatcat
    13081 cgtcgctgta aataaaattg ataaaccagg tgcaaaccct caacatgtta tgcaagaatt
    13141 aagtgagtat gagttaattc ctgaagcatg gggtggagaa acgatctttg ttgagatctc
    13201 agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg atcctgttag tcgcagaagt
    13261 agaagattta aaagcagatc caacacaacg cgcaatcgga acagtgatcg aagcacgtct
    13321 tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa gaaggaacac ttcatgttgg
    13381 tgacccaatc gttgtcggta atacttacgg acgtgtccgt gtaatgacaa acgatcttgg
    13441 ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa atcactggct tgaatgatgt
    13501 acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa aagacagcac gtgcagcggg
    13561 tgaagaacgc gcgaaacgtg cattattaga aaaccgtgct gccacaagcc gtgtgacttt
    13621 ggataactta tttgaaagcc tgaaagaagg cgaattgaaa gaagttaacg taatcatcaa
    13681 agcagatgta caaggttcag cagaagcact ggctgcatca ttgaagaaga ttgatgtgga
    13741 aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca atcaatgaaa gtgatgtaac
    13801 cttagcagca gccagcaacg ctattatcat cggtttcaac gttcgtccta caccgcaggc
    13861 aaaagtgcaa gctgatactg aagaagtcga tattcgtctt caccgtatca tctataaagc
    13921 catcgaagaa atcgaaactg cgatgaaagg gatgctagac cctgaatttg aagaaaaaat
    13981 caccggtcaa atgatcgttc gtgaaacctt caaagtttct aaagttggta cgattgcagg
    14041 tgcctatgta acagaaggat atattcgtag agatagcggt gtccgtgtaa tccgtgacgg
    14101 aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga ttcaaagatg acgtgaaaga
    14161 agtcaaaatg ggttatgaat gtggtgcaat gattgaaaaa ttcaatgaca tcaaagtaga
    14221 tgacgtaatt gaaggattta tcatggaaga agtcaaaaat gactaaaaac tgataaaagg
    14281 aggccaatca ttatggctaa ctaccgtgac cgccgagtcg gtcaagaaat cttaaaagaa
    14341 gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg tggagaacgt aacgatcaca
    14401 gatgtgaaag ttacggggga cttacagcaa gcaacgatct attacagcat tttatctgac
    14461 ttagcatcag ataagaaaac agcacaagat ggattgaata aagcatcagg attgattcgt
    14521 cgagagttag ggcacaatct aagtatttat aagacacctg aattgttctt tgaacttgat
    14581 gaatcagttg tctatggcaa ccatatcgat gaattgcttc gcgacttgaa caaagactaa
    14641 tcaaaaactc ccgcaaatcc tgtgaaatca ggggttgcgg gatttttttg ttttcattaa
    14701 gacgtattac ctaagagttc catgacttta gtcttgcggc gtgtcagcta accaaaaaag
    14761 agaaaaagaa aagtaaaaaa ttcgactttc ttttttctag tagtatataa atttttgcat
    14821 atagataaag atcaagatac cgataacgga agctagattg taatctattt tagtgacagt
    14881 caggtatagc caagtaacta gaagatcagt ggaacctgca ccataaaagt ttgagtttgt
    14941 cggtccgttg acagttaaaa gaaaaatcac gttgaagtta ttgatatttc cgattacgcc
    15001 gcttcgattt ggtcacgatc caaattttgg ataatccctg tagaaattag catagtagct
    15061 gggataccaa ttcacatatt gacaaagata acagagactt ttacataaaa cggatcaatc
    15121 aggaaaggaa ttgctcgatc cagccccagt tttccagcaa tgtgttaact ggcccagcgt
    15181 cattcagcaa attatgcatg atgaggagcg aaatgaactg cggaacagcc attgtgataa
    15241 cgacaagttt tttggatcaa tgtaaaaaac gtcagcgaaa ggaaaaattt cgtggttttt
    15301 tatacctgtt catttggcta gatataaaat ccaaacaaat gaacagaagt gcttattagg
    15361 tttcaattgg gagtatctgc gctagaataa gagtaataat gaaaaggagt gttatgtatg
    15421 gattattcta gtaaagaata ctttgataaa atgacggctt ggtggcgtgc tgctaattat
    15481 ctttctgttg ggcaacttta tcttaaagat aaccccttac tgagacgtac actgaaaccc
    15541 gaagatgtca aaaaacatcc aatcggtcat tggggaacga ttcctggcca aaattttatt
    15601 tatgtccatt tgaaccgagt catcaataaa tatgatttga acatgtttta tattgaaggg
    15661 ccaggacacg gcggacaagt catggtatcg aatgcttatt tagatggaag ttacacagag
    15721 atctatccag aagtaacgga agatgagaca ggaatgcaaa aattattcaa acgattttca
    15781 tttcctggag gcattgcttc tcatgcagca ccagaaacac caggttctat ccatgaaggc
    15841 ggagagcttg gatactctct ttcacatgca gtcggagctg tattagacaa tcctgaagta
    15901 atctctgctg tcgtgattgg tgatggtgaa gcagaaaccg gtcctttagc tggaagctgg
    15961 ttctcaaatg tctttatcaa tccagtgata gatggagctg ttttaccgat ccttcattta
    16021 aatggtgcaa aaatagccaa cccaacgatc ttggcacgta aatcagatgg agaattagca
    16081 aattacttta acggacttgg ctgggaacct ttctttattg aaggaaatga ttcagaaaaa
    16141 ttaaatcctg ttatggctga aaaaatggat caagcaatcg agaaaattaa atccatccaa
    16201 aaagaagctc gcctgaagac agcaacagat gtcgtgatgc caaaatggcc agtattgatc
    16261 gtacgcacac caaaaggatg gaccggtcca aaagaatggg acggggaacc aattgaagga
    16321 accttccgag cacaccaagt accgattcct gtagatcaag aacatatgga tcatgcagat
    16381 gctttactcc gttggctgaa atcctatgaa ccagaaaaat tatttgatgc acaaggacgt
    16441 attttagaag aaatcagaga aatcgcgcct actggtgacc aaagaatggc aaaaaatcct
    16501 atcaccaatg gtggaatcga tccaagaccg cttattatgc cagattggaa aaaatatact
    16561 ttgcaatttg aaaaaccagg ctccgttaaa gcagaagata tgaccgaact tggtaaattc
    16621 gttcgcgaga tcattgagaa aaatccagaa aatttccgta tatttggacc agatgaaaca
    16681 aaatcaaacc gattgaatca agttttcaaa acgacaaatc gccaatggat ggaaaaaatc
    16741 gaaccagaaa atgatgaatg gctttcgcca tctggtcgcg tcatcgacag tcaattatcg
    16801 gaacaccaag atgaagggtt cttagaaggg tacgttctga ctggacggca tggttttttg
    16861 caagttacga atcattcttg cgagtggttg actctatgtt gactcagcac ttcaaatgga
    16921 tgcggaaatc acatgacctt tcttggcgaa atgattatcc ttcgctaaat ctcattgcaa
    16981 gctcaacagt cttccagcaa gatcacaatg gatatagcca ccaagatcca ggaatattga
    17041 cacatttggc agaaaagaag gcggagttca tccgtgaata tctgcctgcg gatgcaaata
    17101 cattgctcgc agtcatggat aaagcattcc gctcgtcaga aaaaatcaat ttgatcatct
    17161 cttccaaaca tccgcgtgct caattttact cagcagaaga agcagccgtc ttagtgaatg
    17221 aaggcttgaa aatcattgat tgggcttcga cagcaaaaga ggaggaacct gaattggtca
    17281 ttgctgctgc aggaacggaa tctaacttag aagcattagc agctgtaact ttgctgctgg
    17341 aagaattccc aaaactaaaa attcggttca tcaatgtcgt agatttattg aaattacgtc
    17401 atccaagtca agatccgcgt gggttgagcg atgaagaatt tgatcaatat tttacaaaag
    17461 acaaaccgat cttatttgct ttccacggtt atgaaacatt ggtccgcacg attttctttg
    17521 atcgtcataa ccatcattta atgattcatg gctacaaaga aaatggagat atcacgacac
    17581 catttgacat gcgtgtagta aatgaacttg atcgttatca tttggcaaaa gacgcagctt
    17641 taaaaatcaa aggaagtcaa gcagaagatt ttgcgaaaaa aatggatcag aaattacaag
    17701 aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc agaagtattg gattggaaat
    17761 ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc aaaatagaat tttttacatt
    17821 gattaagaaa ctgaaaaaat aaataaaaaa aacggaaaaa agactggaag aatggccagt
    17881 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
    17941 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaagcataa
    18001 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
    18061 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
    18121 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat tacctttatg gaaagaacgc
    18181 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
    18241 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
    18301 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
    18361 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
    18421 gatatgtctt tatctgaaaa aaaatagatg aagtgatggc ttcatttatt ggtgagatca
    18481 cccagatacc accaatgtac tcagcggtaa aagtcaacgg taagcggtta tatgaatatg
    18541 cccgcaataa tgaaacagtg gagcgtcctg ttagaaaggc tcagatttac cgttttatcc
    18601 ggacaagtga attgacaaga gacgaagtga caggaaccat ttcttggcgc ttcgaagtag
    18661 aatgcgggaa aggaacctat gtccgcacat tagctgtaga tacaggcaag aaacttggtt
    18721 accctgcgca catgtctgat ctgacccgaa cagcaagtgc cggtatgtcc cagaaggatg
    18781 ctctgacatt agaagaagta gctcaagctg tgaaaaatgg gacaatcgat gattgtttgc
    18841 tgccaataga aaaaggagtg gaacggttca aacgagtaga tattactgat gaagtatggc
    18901 agaaagtaaa aaacggtatg agacttgatt atactgtatt tgcgctccca gaaatgccgt
    18961 cagaagaaat tgctttgttt tataaaggaa aagttgtcag tatttatcag ccaaacccga
    19021 aagaaaaaaa caaattaaaa cctagtaaag tattgagaaa tgaggaataa cgaatgaata
    19081 tcatcaagat cagacatcct taccaatcag aacaaattcc agaagacgat gtcgttttag
    19141 tgctgggatt tttcgacggc gtgcatatcg gacaccagaa agtcataaag acaggtatgg
    19201 aaattgcaca aaaagaagga ttgaaattag cattgatgac tttcaatcag catccatcta
    19261 ttgtctttaa aaagatcgat cctagcagcg tgaaatattt gactagcctt aagcaaaaag
    19321 aagaaaaaat ggccgctttg ggtatagatt atttatatga agttgatttt acttcttctt
    19381 ttgctcattt agcaccgcag gaatttgttg atcaatacat cgtagggtta catgcaaaat
    19441 atgctgtttc cggttttgat tatacgtatg gaccaaaaga cattgcagat gtcgctcatt
    19501 ttcctgaata tgccaaagga agattcaaaa tcgtcacagt ttctaaagaa gaagaacaag
    19561 gtgaaaaaat cagctctacc cgtattcgtg gattactaga cagcggacaa gtaaaagaag
    19621 cagcaaaact attaggttgc acatatgagg tagaaggggt agttgtccat ggcgatgcac
    19681 gaggcagact actaggattc cctacagcaa atgtcaaagt aaagagcacg gttcatttgc
    19741 ctaaagaagg tgtttatgtg tcggaaataa aaattgggga aacatggtat ccagccatgg
    19801 gttcaattgg acataatgat acattcggtg aaggacggga gctgactgta gagttgtata
    19861 ttttggactt taatcaagat atctatggtg aacatgtcgc ggtacgctgg aatcaatttt
    19921 taagagatca agtgaaattt tctagtgtag aggaattgat tgaacaactc aaggaggatg
    19981 aacaagcaac agctgaatat ttttctcagc aataaaacat tatgcataaa atctcttgtt
    20041 tatgtataac tagcaattac aataaaatgg gtgataaata agcccaggaa aggggctgtg
    20101 acaaaagtct tgtcacagcc tttttttcca aataaatggt ttttcagaag tagcttcttc
    20161 ggaaataagc cgaaatttct tgaagctgac cacttctgtc acaacctctt tctttataga
    20221 aataaaagga cactagcaat tcatgaaaac gattgctaga gcgtatcatt tgctttagac
    20281 tatatatctt ggtgtacact cactttaagg cggaaataaa agttatccgt aataacaggt
    20341 ttaggtatga aaaaggagtg ggtactatgt acaaggaatt agaaaacaaa gtcgcagtag
    20401 taactggtgg ctcaaagggg attggaacag cgattgctca acgattcggg aaagaaaaaa
    20461 tgaatgtagt tgtcaactat aactcggata aagaaggagc agaaaaagca gtcaaagcaa
    20521 tcgaagaggc aggcggaaaa gcagtctctg tacaagcaga tgtcagttct gaagaaggaa
    20581 tacagcgttt acttgatacg gctttagaaa attttgggac actggacgtt tgggttaata
    20641 atgctgggat ggaaaatcag catccaaccc atgaacttag ccttgaagat tgggaaagag
    20701 tgttaaaagt caatttgact ggcgttttta tcggaacgaa agcggcttta aattacttta
    20761 cagagaataa taaaaaagga aatatcatca atatgtcttc tgttcatgaa cagatcccat
    20821 ggccaacgtt tgctcattac gctgcttcaa aaggcggggt aaaattattt accgaaacgg
    20881 tagcaatgga gtatgcaccg aagggaatca gaatcaatag tataggccct ggtgctatcc
    20941 gtacacctat taatgcggca aaatttgatg atcctgaaca aaaagaactc ttggaaagta
    21001 tgatcccact aggacgcgta ggtgaaccaa aagaaatagc ggcgacagct gcatggttgg
    21061 cttcagacga atccagttat gttacaggta ttacactatt tgcagatggt gggatgactt
    21121 tatatccttc tttccaaggt ggaaaaggat aagggtgaat aaaaatgggt ttattgattg
    21181 cattgatccc ggcagttggc tgggggatcc agccactgat tttgaaaaag attggtggca
    21241 gaccaacaaa tgagatatta ggaactggta ttggagcggt gcttgtcggc ttactcgttc
    21301 aattattcgc ttctccagct ggtatttcag tgactacatt tttgattagt ttactttctg
    21361 gtgcattttg ggttattgga caaacaggac agtatacatc tttcaacttg attggtgttt
    21421 ctaaaacgat gccaatttcg actgcattac agttagtcgg aacctcgttg attggtgtct
    21481 tcgcttttgg agaatggtct ggaagtatgg ggaaaatcat tggagcagca gcaatcattc
    21541 ttcttgtatt tggttctgct ttaacggcaa tcagtgatga tcctggaaaa aaaggcggta
    21601 tcacaaaagg aattagtcta ttagcatcaa caagtattgg ttattgggta tacagtgcat
    21661 tgcctaaact agttgatgca agcggacttt ccattttctt tccgcaaatg atcggtgtct
    21721 ttttaggggc agtgatttat gtgattttcc gtaaaccaac cgcattttct gaagacaaga
    21781 gttggaaagc aacgatcgtt ggagccatct ttagtgtgtc agcattggct tatatttttt
    21841 cagcaaatga aaatggggtt gctactgcat atatcattac acagctaaac gtagttatct
    21901 ctacattagg cggtatgtac atcctgcatg aaaataagac accaaaagaa aaaaaattaa
    21961 cgattatcgg attattgctg atcgtagtag gaagcatggt cacggtcttt atttgaggat
    22021 aaagatggta aaatttcatt tattaaaaaa atatttgttt gaaccaaaca gtaagtactt
    22081 ataaaaaaat agcgctagaa tttccaacat ctctggaatt tctagcgctt tttaattgtt
    22141 taagatacaa gggaaatatc tgtctggatc aataattttt cattgatcca ttgactaaag
    22201 tttacgacat aattgaagtt accaccgatt agtatctccg catgattgtc tttataaaga
    22261 tcgtattgta aaagacggtc ttttttattt gcgtaaacat cgttatggta ctggactaat
    22321 tgttctcgga attgattgat tgctgtaaaa tcacgatatt gaaggttgaa acgcatcgtg
    22381 attggtgcat ttagcatgaa tcgctgcatt tcttttgctg cttcctctgc ttgatctccg
    22441 tataggaact caaaaagatt acgttcagaa aatttccatt gcccgccaac ctttgttcct
    22501 tttagttttc cttcatgaag ataatttctg atagaacgtg tagtaacacc taattgttcg
    22561 gctaattcat ttactgagta aaaatctttc aatgttcacc ctcctatgtg aatggttcta
    22621 ctgacttagt tgatatttgc ttaatcacga aatgtagaaa gaacgatttt aattaaggtc
    22681 attgtaaaaa tcaagtcaga aaattctaac ttttatttct ctaaagaaat tcgttctctc
    22741 ctaaagtgat attatattta aatatacctg aaaataatgg gcagtattat gaaagatcac
    22801 tgatattttc tcgtattttt tccttattca aaagttttgc aggaaaattg ctgaaaaaat
    22861 aattttacta tgtagaaaat tcaaagccat tcaatcagtg atttttttca gcacaagaaa
    22921 gactccttat aaaaatagca gatactttgt caattaagtg tttaaccagg gatgataaaa
    22981 aggataggat tgaacaaaac ctgtataagg tgatgaatag tttcctcgcc agtaaaaggt
    23041 ttattcgtat aaagctttat cgccaaaatt tatcatgacc ttttttatct tcaatattaa
    23101 atgaaatcaa ttgttcaatc aattcccagt tgattacact tttccaagga actttgatga
    23161 cgttatccgt atatacgtag cctgcttctt ggatattttt ttcaagggca cgaatcgctg
    23221 ctttttccgg tgcgatagaa aaatgatttt tagcaaaact aaatgcaata ataaaggtac
    23281 catggtcagt aaacataggt tggttccatt ttacagttgt gtctagctgg ggatacgttt
    23341 ctgttatctt agcaaaaagg tctttgatct tttgttgatg ttctttttct ttgatttgat
    23401 tgatgatcgg ctgaaatttt tccattctaa aacgcctcca ttaagtggtt tgatttattt
    23461 taccagaaaa acaatagtta tgtataaaaa acgaacaaaa cacaagtaat gttgtaaccg
    23521 ctatatttat atgataagat gtctcaaata ataaaataga aaagagacat aaaaatgatt
    23581 gacttggcaa agaaattctt acacgaaaat atatattttg agttgtatca tcatcgagcc
    23641 atttatacaa atgaagatgc tttggtaata aagaaagaac aaggatttac aggtacagag
    23701 accaaaagct tattcttaaa agataaaaaa gataataact acgtatatct gacttttact
    23761 acgaaacaaa cggatttcaa agcattaggg aagatcgtcg gtaaaaggct ttctattgtt
    23821 tcatcagaac gcatggaaga acaaacggga caaaagcagg gcgctgtttc tccgtttggt
    23881 tatgaaaaac agattccgat tattattgat gaagagctct tatcaaatga aaaacttgtt
    23941 tttgcaccag gtagaccaga tcaaacgatg gtcatccaaa cggttgactt aaacaaaatc
    24001 atccagctac ttgagatgcc gatatttttg atgccggcaa acgaaaaaaa caattaacca
    24061 agtaaaatag tgcatacttg gaggtgaagc aacatgataa gttatccatt attagaaaga
    24121 aatgcaacga ttggggttac tgcaccatcc tcaggcgtat cagaggaaca ccattcgtta
    24181 ctcgaatcag ctttaaagag aatggaagaa aaatctttcc aagtagttac tggaaagacg
    24241 ctttggacgc aagataagga aaaatcaggt ccagcaagaa aacgagcaga tgaattcaat
    24301 caaatgatgc aagatgaaaa tatccaaatg atctttccga cttggggagg agaattattg
    24361 attgagattc tcgattttgt cgattttgat gctctacaac caaaatggat agtaggttat
    24421 tcagatatta gtacgttgct acttgctgtc acattaaaaa cagggatagc cactgcacat
    24481 ggaactaacc ttattgattt aagaggagag tacttggatg aaacgacagc aatgtgggaa
    24541 agtgtactga aaacaagaga aggagaatcg attctacagc aatcttctga aaagtatcaa
    24601 aaagtgtggc cccatgagca ttcatcatct tctgttttcc atctcactga accaacaaat
    24661 tggcaatcag tcagcggaga tgcagtaagg atgaagggac gattattagg tggttgtatt
    24721 gatacgatca ggcatttaat cggtactcct tatggagatg tccaatcttt taaaagaaag
    24781 tataatgatg gaaagccgat cttgtggttt ctagaaaact gtgagctatc gtcagttgat
    24841 ttaagaagat cacttgttca gatgaaactg gcaggctggt ttgatggcag ctcggggatc
    24901 atgttcggta gaagtgcggc agaggatttg ttagattata aaatgaagga tgtatacgaa
    24961 gatctgtcaa aggaattaaa actaccgatc ttgtatgata ttgattgtgg tcatgtaccg
    25021 ccacaactga cattgattaa tggtgcctat gcagaagtag cggtagaaga tagaaaagcg
    25081 actgttttgc aaacgtttat ttaaaaaatt ttttcctgcc ttggagtcta tcaaagacca
    25141 ataaatctaa atagaaaaga aagtgaaaga agctgacaaa atgctgtcag cttctttttg
    25201 tataacatat tagaaagtct aattatctat aactgtcaca tcgattaact gtttcacgtc
    25261 attgggattt cctgtaggaa tagctactac agatgcatta tcaatgattt cttgagttgt
    25321 ttctctaatg aacgctggat aagatgtttg atctgcaggc atttgccaat gtgaaggaac
    25381 tggataacta gtgattgtcc cgtcatggtt acttgaatac actaaaactc cctggtaact
    25441 gtatttccca gtgagcataa ccacatcttt cggatagact gcactccctg catcataagg
    25501 ggcgataggt gttcctgcag gataatgaat aacatgtaat tcgaaatcac ccgatgtatc
    25561 tctaaaagta ttacctctaa tagctagcca aacacgtgcg tactcaattt gtagatcaga
    25621 atatcctgct aacggatcta ttgactgaga agactgtgag gaggttgtgt ctattgttga
    25681 agaagaaata tttgtctcaa ctgtactact agatgtagaa gaagtattgg gctctgagac
    25741 tttactgctt gttgaagatg tattcgtttc agtagaagct gatttctgag tacttgattt
    25801 ttcagctgaa ctggtttgtc tgattgaact tttgtctgtg gtcgcagatg gatcactatt
    25861 ttctttacta ggagacgtac atcctacaag gaataaagag accaacaata aaataaaacc
    25921 taactttttc atataaaata cctcattatt tttttaatac cagtatagca taatcctgaa
    25981 tttttgaatc cgtaaaacct tgtctatgaa tataaataga gtgtatcttc tttgataaat
    26041 gtcagaaaaa gtgtgacaaa taatcatcag taaaattact tgtcacacta ttgataagga
    26101 gtatttaatt gaagagaatg attaggagaa aggatctccg tactttttat agtaatcata
    26161 gaatccaagt ttgtatagac gaatcgtttt ttctcttatt tcttctgttt caaaatggac
    26221 tgcttgaaga atcaagtctg tgaattctat aggagctgtt ccattcgcag taatcaaatt
    26281 acgatctttc acagcttgtt gttctataaa atcttcttta ttctgataat tttccgattc
    26341 taaccaagga aattgcgcat ttccggtgtg tttgtaacct tgcaataatc cgctctgagc
    26401 taaataatca actgccccac aaatagcagc tactggtacg tctgttttta acaatttatc
    26461 gattatttga tataaatgat gattttttat gtcccatgaa ttgccaccga tcataataaa
    26521 aaggtcaact ttttcaggta tttgttcgat taagtaatcg atttttgttt ttaatcctcc
    26581 aatggaagtg acctctgatt ggatagaagc agttttgttt tgccatttct cattttggtt
    26641 tagtgtgcta gtaatataag caccttccca atcggcatat tcctctaata gaaacgtaat
    26701 cgcagttttc attttcaagc tctcctttac tatctataac aaggacaaca gttttaacct
    26761 acagacattc attaaaattg ggtagcgaca gcgaaaacaa caattcttaa gttttagaca
    26821 gagtatacag atagtcaact taattaaata cgaactaaat aaaaatgaca ggtaatcatt
    26881 tttgtttacc agcattataa cattttgcat acatggatga caaaagaacc aattcccact
    26941 tgcttgagta caatattaaa agaaactgga aggcaaaaag taattataga aacgaaaaat
    27001 aaatcatatc gggcactcag acatgaaaac aaccctagag atatataact aggtaaccag
    27061 tttaatgaaa gaaaaaatca tccaagaagt cgattcttgg attttttagt ggatttgttc
    27121 gccaaatacg tttagtatgc gtatatgaga ggttgtaagc aaagcgttgt ggtctgagca
    27181 cttaaggagg aatctgtgaa aatagctttt catatttttg cagattttga cttaatgccg
    27241 aaggtcagct ttgcgattac cgtttatcga gaggctgtaa aagcaccgtg tagctccgat
    27301 gacaataagc aagcattttt agagctaggt ttccttgatt ctaaatatgc ggcttatttc
    27361 aatgagttgg tgcttgaacg cgattttaag aaggtcgaaa acaggcacga cttttcgacg
    27421 taaggagaaa gaatttcaat gatagaaaca ataaatcccg cagaaaacaa ggtttctgcg
    27481 tacattcata tcccattttg cgagcatatc tgctattact gtgattttaa taaagttttt
    27541 ttagaagggc agccagtaga tgagtatgtg gaaatgctgc tgaatgaaat gaaaataatg
    27601 ctagaaagtc atccgacagc gaatttagaa acactttacg tagggggcgg gacaccaact
    27661 tctttatctg ctaaacaact tgatcgactg ttatcaggtg ctagagagat cctgcctttt
    27721 caaaatggta atgagtttac tgtagaagcg aatccaggtg atttgacccg tgaaaaactt
    27781 caagtaatga aaaactatgg cgtcaatcgt ttatccatgg gagtgcagac atttgataat
    27841 cgcttattga aaaaaattgg acgtaaacac accgcagaag atgtgtatca aactatgcaa
    27901 tttttagaag cagaaaattt taccaatgta agcatcgatt tgatttatgc attaccggga
    27961 caaacactag aaggatatct ggatactttg gaaaaagctt tggctttgga tctgcctcac
    28021 tattctttgt actcattgat cctcgaaaac aaaacaatgt tcatgaactg ggtacgacaa
    28081 ggaagactcg aactgcctga tcaggaaacg gaaaccagaa tgtttgaaga gacgattgaa
    28141 gcaatggaaa agcatggacg tcaccaatat gaaataagta actttgggct ctccggacat
    28201 gaatctcaac ataacctcat gtactggaac aatgaccact attttgggtt tggagctggt
    28261 gctagcggtt atcttggaga tattcgttac cgcaacaaag gaccaatcca acactattta
    28321 cgcccattac gagcaaatga acgcccggta ttggaagaag aaaaattgac acgaaaaaat
    28381 caaatagaag aagaaatgtt cttaggacta agaaaaaaaa cagggattac taaaaaacat
    28441 ttctatgatc gctatcaaaa aacaattgaa tcggtgtacg gtgatgtgct aagggaatta
    28501 gagcaagaag ggttacttgt taatgaagaa gaccgtgttt atttgacacc aagagggacc
    28561 tttttgggga acaatgtgtt tgagcgcttt ttgtttgaaa aatagtttaa agaatatttg
    28621 ttttttatct gaatttaaat aaaaaaagtt caaaaataca ataaaggact attgaaacgt
    28681 tgatataact ggatttacat caattattta gaattagcac tcttgtgttt agagtgctaa
    28741 aaaatggagt ctttctcttg acaggagaag ggcttcttgc tatatttata agtggattag
    28801 cacttagaat atatgagtgc taatgagagg tgaggaagat gttaacaaag cgacaaaacg
    28861 acattttacg tctgatcatc caacactata cgagtagtgg cgtgcccgtt ggttccaaaa
    28921 cattgatgtc tgagggagtc aaggccagct cagcaacgat tcgcaatgat atgaaagcat
    28981 tagaagatga aggattgctg ttgaagactc actcttcttc tgggcggatt ccatctgctt
    29041 taggctatcg ttattacgtg gatcatttgt taaaacctgc tcgcgtagca gaagatgatt
    29101 tgctgctgat tcgtcagtct ctaggcagag aatttcatga aatcaatgag attattaagc
    29161 aatctgctga gatattatct gagctgacaa gctatacgac tttttcattg ggacctgaaa
    29221 taaaagaccg ccgactgact ggctttcgta tcgtaccctt gaataatcga cagattattg
    29281 ctatcgtcgt aacagatatg gggaatgtag aaagccaagt gttcactctt cccgaatata
    29341 tgggaagtca ggaactagaa aaaatggtac agattgtaaa cgaccgtcta gtcggagatc
    29401 ctctgatgac ggtgtaccat aaacttcgta cggagattcc gatgattctt cataaatatt
    29461 tccaaacgac agaagggatg tctcagttat ttgacaccgt tcttggtcat gtttttgaag
    29521 ataaggttta tgtcagtgga caaatgaatc tcttggatta tgagccccgt caagatcttg
    29581 atcagttcaa atcgatgttt tcttttatga aagactcaga cggacttaca caaatgatca
    29641 ctccgatgga tagcaatatt catatcaaga ttggtgctga attaggcaat gatttattgc
    29701 agaatatgag tatgattcaa gcaagttacg agatagatgg tcatggaaga ggtacaatag
    29761 ccttgttagg gcctactagt atgccttatt ctcggatgtt cggtctagtc gatgtttatc
    29821 gaagagaact agcagctaaa cttgctgatt actaccgttc gcttgacttg tcaaactcgt
    29881 aatgtgaata acgaagctat ttgcagctga tcgttctctc tttcttagta agagcaatca
    29941 aacagctgac tgaataatga aaggaagact gtatcgtgaa agaaaatcaa gaagaactag
    30001 ataaagaaat gacagatgct cagccagaac cggaaatcga tgtcgaagct gcagaagatt
    30061 cgggtatttc agaagctgaa gcggaagaat tcgaaacagc gaaattaaaa gctgagttag
    30121 aagaaatgga agataaatac ttgcgggctc gtgctgaaat cgcaaatatg gcaaatcgag
    30181 gaaaaaatga gcgtgagcaa ttacaaaaat accgttcaca agatttagcc aaaaaattac
    30241 ttccatcaat tgacaacctg gaacgtgcat tagccacaga agtgtcagat gatcaagggg
    30301 caggtttgaa aaaaggcgtg gaaatggtct tggaaagttt gcgtaacgca ctggaagaag
    30361 aaggaattga aaaaatccct gctaaaggag aagcctttga tcctaatctt catcaagcag
    30421 tacaaactgt tccagcgact gaagatacac cagccgatac gattgtagaa gtactacaag
    30481 aaggctacaa attacatgat cgcgtgttaa gaccaaccat ggtcattgtt gcacaataat
    30541 tgtgcggata taaaacttac actttcaatt aaagaaggag acgttaatta tgagtaaaat
    30601 tatcggtatt gacttaggta cgactaactc tgcagtagca gtattggaag gaaacgaagc
    30661 aaaaatcatc gctaacccag aaggaaaccg tacaacacct tctgttgtat cattcaaaaa
    30721 tggagaaatc caagtagggg aagttgcaaa acgtcaagct gtgacaaacc caaatacgat
    30781 ttcatcaatc aaacgccata tcggtgaagc aggttacaaa gtagatgtag aaggtaaatc
    30841 atatacacca caagaaatct cagcaatgat ccttcaatat atcaaaggat tcgcagaaga
    30901 ctatttaggt gaaaaagtcg aaaaagcagt tatcactgtg ccagcttact ttaatgatgc
    30961 acaacgtcaa gcaacaaaag atgctggtaa gatcgctggt ctagaagtag aacgtatcgt
    31021 caatgaacca actgcagctg ctcttgccta tggtttagat aaaacagatc gcgacgaaaa
    31081 aatcttagta ttcgaccttg gtggcggtac ttttgacgta tctatccttg aactaggaga
    31141 cggtgtcttt gatgtattgt caacagctgg cgacaaccat ctaggtgggg acgactttga
    31201 taacaaaatc atcgactaca tggtagcaga attcaaaaaa gaaaacagca ttgacttgtc
    31261 tcaagacaaa atggctttac aacgcttgaa agacgctgct gaaaaagcga aaaaagattt
    31321 atctggcgta tcaagcacac aaatcagctt gccatttatc acagcaggag aatctggacc
    31381 attgcatttg gaaatgacat tgacacgtgc aaaatttgat gaattaacag ctgacttagt
    31441 agaacgtaca aaagtaccag tacgtcaagc gctaaaagat gcaggtttat cacaatcaga
    31501 aatcgacgaa gttattttag tcggtggatc tactcgtatc cctgcagttg tagaagcagt
    31561 acgtaaagaa acaggaaaag aaccaaacaa atcagttaac cctgatgaag tagttgctat
    31621 gggtgctgct atccaaggtg gcgtgatcac tggtgatgtg aaagatgttg ttttacttga
    31681 cgttacacca ttgtcattag gtatcgaaac aatgggtgga gtgttcacta aattgattga
    31741 ccgcaataca acgattccaa caagtaaatc acaagtattc tcaactgcgg cagataatca
    31801 accagcagta gacatccatg tattacaagg tgaacgccca atggctgctg acaacaaaac
    31861 tctaggaaga ttccaattga cagatattcc agcagctcct cgtggtattc cacaaatcga
    31921 agtaacattc gatattgata aaaacgggat tgtaaatgta tctgcgaaag atctaggtac
    31981 acaaaaagaa caaaaaatca cgatcaaatc ttcttcaggt ctaacagacg aagaaatcga
    32041 acgtatggta aaagatgcag aagcaaatgc agaagcagat aaagctcgta aagaggaagt
    32101 tgacctacgt aatgatgtag atgcattgct attctctgtg gacaaaacat tgaaagaatt
    32161 agaaggcaaa gtggacgaag aagaagtgaa aaaagcagaa acagctcgcg atgaattgaa
    32221 agccgctgtt gaagcaaaca acatcgaaga aatgaaaact aaacgcgatg cattgaacga
    32281 aatcgttcaa aacttgactg ttaaactata cgaacaagcg gcacaacaac aagctcaaga
    32341 aaacccagaa gcagctcaag gcggagcaga tgatgtcgtt gatgctgatt ttgaagaagt
    32401 cgatggcgac gataaataat cagttcactg aatttcgaga gaaggccaaa gttgctactt
    32461 tggctttttc ttttgaaaaa tagtttgaag tatcggtttt tgtttcaaac aaaatatgat
    32521 atgataacaa gcgacgtgaa aatgatgaga accaatagga ggaaagccta tggcaacaaa
    32581 acgtgattat tatgaagtct tagggctctc aaaaggagca tcagaagatg aaataaagaa
    32641 agcttatcgt aagctttcga aaaaatacca tcctgacatc aataaagaac ctgatgcgga
    32701 agaaaaattc aaagaagtat cagaagcata tgagatttta agtgacccgc aaaaacgcgc
    32761 tgcatatgac caatacgggc atgcaggcac agatccaaat tacggtgctg gtggagctgg
    32821 tggcggattt ggtggattcg gcggcttctc tggtggcggc ttcggtggtt ttgaagatat
    32881 cttcgactct ttctttggag gaggcggtcg gtcagtcgat ccaaatgcgc ctcgtcaagg
    32941 tgctgatttg caatatacgg tcgacttgtc ttttgaagaa gcgatttttg gtgtagaaaa
    33001 agaaatcaag tataatcggg aagaaatctg tcatacttgt ggtgggaacg gtgcaaaacc
    33061 aggcacacaa ccaactactt gtcataaatg tcatggtgca ggtacgatta atgtagaacg
    33121 ccaaacacca cttggacgag tgatgagccg tcaaacttgt gatgtttgtc atgggacagg
    33181 gaaagaaatc aaagaacctt gtccaacctg tcatggctca ggacatgaga agaaagcaca
    33241 ttcagttaaa gtgaatgtac cagctggtgt agaagaagga caacaaatgc gtttagctgg
    33301 tcaaggagaa gctggagaaa acggcggacc atttggtgat ttgtatgtgg tcttccgtgt
    33361 agaagaaagt gatatctttg atcgtgaagg ttctgaaatt tactatgaat tgcctttaag
    33421 ttttgtacaa gcagctttgg gggatgaagt ccaagtacca actgtccatg gagatgtgaa
    33481 attgaagatc cctgctggaa cacagacagg tacgaacttc cgtttacgtg gaaaaggcgc
    33541 tccgaagtta cgtggtggtg caacaggcga ccaacatgtc aaagtaaaac tgatcacacc
    33601 gaaaaatcta aatgaacaac aaagagaagc attacgtgca ttcgctgaag ctggcggcat
    33661 caaagttgaa gaacagcaag aagacggttt cttcgataag tttaaagatg cttttggcgg
    33721 gaaaaagcgg aagtagtttt tctagtaaaa acgctaagtc attaataatt aaaaaccaga
    33781 cgaacaatca tatgttcgcc tggttttttt gtagttgtca tgtttgtacc aacaaaaaag
    33841 ccagctttag aatatgataa tcgtcatagt ttctaggggt ataaccagtg agagaatgga
    33901 acttgttcaa tcgatattgc aaggtgtttt tatgaataaa taattcttct gagcaatttt
    33961 tgatgcttcc gttattttta ctgaagcatg ccaaagtctt cttgaacaaa tgtacttctt
    34021 cttctgtcag gcttttcaaa atacgtttta catataatgt tcgttgatct gacgggattg
    34081 ctggtaaaac gatcccgagg tctaaatctt caaaaaagcg gatctcgttc acagattgat
    34141 ggaaagctat ccattcgcaa acttgtttgg catgaaggaa agattgcggt gtctcttgct
    34201 ctgtataaca aagcgaccca attccaaagt ttagctgtaa ctgatacatt ttattaaact
    34261 ttgtctggat tttatcaagc aatgatagca atgaggctct ggaaatccga ttgactagga
    34321 taatgatttg ttgtctatgt acagtgagca aagcctgtcc gtaataagag aagacccttt
    34381 cgagcaactc caataaagga ttgatccgtt cttgacacaa ttgctgattg tcagaaagtt
    34441 gtcccacaac tacataatat ggagaagaaa aatcaagctc aattaagtga gggtggtatc
    34501 gactatgtac ttgatatcat caatgaaccg atggtacttt cttatgcgat ggaacaggta
    34561 gagcgattgc ttgtttgtgc aggaaaacaa gcgatgcggc tgatacgatt aggtgagaaa
    34621 ataagaagat aaaaatacac ggtcccttca tggcgaccgt gcatttttgg tttttattaa
    34681 atatttaata ctttattcaa gaaatctttt gtccgttcat tttgcggatt ttcaaagatt
    34741 tgttgaggcg ttccatcttc taaaaagttt ccgtcgtcaa tgaacatgac gcgattggcc
    34801 acttctttag cgaaccccat ttcgtgcgta acgataacca tcgtcatacc ttgttctgct
    34861 aactttttca tgacgttcaa gacatcaccg accatttcag gatcgagtgc agaagtaggc
    34921 tcatcaaaaa gcatgatgtc agggttcatc gctagagcac gagcaatagc cacacgctgt
    34981 ttttgtccac cagaaagcat ttcaggatac atatcttttt tatcttctag accaactgtc
    35041 tgcagtaatt tgattgcttt tttctctgct tcttctctag attctttctt caggtccatc
    35101 ggtgctaaaa tgatgttttc caaaatagaa agatgaggga ataggttaaa gtgttggaat
    35161 accattccga tatgttgtct caccaagttg atatctgtgc ttttatccat caagtgggcg
    35221 ccgtcaataa taatttcacc gtcagaaggt tcttctaaac gattcaagca acgcaagaat
    35281 gtactttttc ctgaacctga agggccaatg atacagacga catctccttc gttgatggat
    35341 acattgatgt cgtttaatac tgtgttatcg ccgtattttt tgactaaatg ctcaactaag
    35401 attttttctg ccatcttact tcaccttctt ttctaaaact tttgctaatt ttgtcaatgc
    35461 tgtgatcaaa atcaagtaca tgatcgcaat gatcaagtaa acatacgtac tttgtgttgt
    35521 acgtgcaaca atgatcttac cagtttgcag caattcgact acaccgattg cggaaatgat
    35581 cgttgtgtct tttaatgaga taacgaactg attgacgaat gatgggatca tgatgcgtat
    35641 cgcttgcggc aaaatgattt tttgcattgt ccgattataa gaaagaccta aacttctgga
    35701 agcttccatt tgtccgacag gaacggcgtt gatcccgcct cgaacaatct ctgcgatata
    35761 agcacttgcg ttaagagtca aggtgataac accagcaagg aagtcaggaa tcgtaaatcc
    35821 aacgatacca ggcaacccaa agaaaataaa gaacgcaaga accatcattg gaattcctcg
    35881 gatgatatcg acataaatac tagcaattgt gcgaagagcg cgtattggtg ctacactgaa
    35941 taatccaaaa atcacaccga ttactatagc caaagcaaag gagatcaaag ttaggagaat
    36001 tgttctccaa agtcctttca acaatgcttg atagttgttt tggataagtc caagaaatgt
    36061 tgattcatca gcagtggaag actcactgga agtttctgaa ccatccgcta catattcttt
    36121 aataatctta tcgtattcgc ctgttctttt taattctttc aatccttcat tgaacatatt
    36181 aagaagctca ggattttgtc cttttttaac ggcaaatgca tattttccgc cagattcttt
    36241 tttgatcggt gtttccaatt cttgtccttg tttgatcgca taaccgatta ccggataatc
    36301 atccatcatt gcatcgattg cgccaacttt taacccgtcg tacaattgat cagcagcatc
    36361 aaaatatttg atagaatagc catattcaga ttcatggtcg cttaaaaatt cagcactttc
    36421 ggtaccaact tttgctccga ctgtttttcc tttcaaatct tcataagact tgatcgaagt
    36481 atctcctttt ttgacagcta attggatccc gctatcaaag taagaatcag aaaaatcata
    36541 gcttttcttt cgttcatctg ttacagtcat tccggcaatc attccgtctg cttgtcctga
    36601 ttccaaagct tgcaaagcag cagagaaacc acggtagtcc attcggatgt taaatccttg
    36661 cagtttggcg atgttttcca tcaattttac atcgatgcct tcgtactcgc cctttgcgtt
    36721 ttgaaattca aatggagcaa aagcagagtc actggcaatt acgtatacgt cttttttggg
    36781 ctctattttt tgcattgtct cgcttgtgct tgactctgcc gttgtagtgt tgtcagcttg
    36841 tgcaacatga ccaaacgcaa atgtcacaag tcctgtcatt aagaaaacaa atgagtgaaa
    36901 taaatgcttt cttttcataa tcaacctcct aaatttccaa tattttatta ttataggcga
    36961 aaagtgctga atcagacaag actgtaagct cgaaaaaatg aaaaaaatgc gagtttctaa
    37021 cattttatgt tataaaacat aacatgtata ataagataaa aaagtggcga aagtatgttc
    37081 ggtgtggtat aattttttag taaggaatgc ctttgctttc cttttctttt tattggtcgt
    37141 aaaatgaaaa ggaatgaatg aaaaggagga cgaagaatga ttgagagaga tacctcccga
    37201 cactttgagc ttgtctcgaa ataccaaccg gctggtgatc agcctgaagc gattaaccaa
    37261 ttagtggatg gcgtagtcgg cgggaaaaaa gcacaaatct tattaggtgc gactggaaca
    37321 gggaaaacat atacgatttc caatcttatt gagaaagtga ataaaccaac actgattatt
    37381 gcccataaca aaacattggc tggtcagctg tacggggaat tcaaggaatt tttccctaac
    37441 aatgctgttg aatattttgt ttcttattat gattattatc agcctgaagc ttatgtgcct
    37501 tcaagcgata cgtatatcga aaaagattca agtgtaaatg atgagatcga caaattacga
    37561 cattcggcta ctagttcatt attagaaaga aatgatgtga tcgtcattgc atccgtttca
    37621 tgtatttttg gattaggctc gccttttgag taccaaaaac aagtagtttc gattcgacag
    37681 ggagcagaac tagatcgcaa tcagttgatt cgtgacttag tcagtatcca attcgaaaga
    37741 aatgatatag attttcagcg cggtcgtttt cgagtgcgcg gtgatgtcgt ggagattttt
    37801 ccggcttcaa gagatgaacg tgccttacgt gtggagtttt ttggagatga ggtcgaacgt
    37861 atccgagaag tcaatgcact gacaggtgaa gttttaggag aaacggaaca cgtagcaatc
    37921 ttcccggcga ctcactttgt gacgaatgat gaacatatgg agcatgccgt agcgaatatc
    37981 aaagcggaat tagaacagcg tttaactgtc ttgcgaaatg aaaacaaact tctggaagct
    38041 caacgtttag aacagcgaac caattacgat attgaaatga tgttagaaat gggctacacg
    38101 tcgggaattg aaaattattc tcggcatatg gacggacgta aagaaggtga accgccttat
    38161 acgctattgg atttctttcc tgaagatttt ttgatcgtgg cggacgaatc gcacgtaacc
    38221 atgcctcaaa tcagaggaat gtataatggt gaccgagcga gaaaacagat gctagtagat
    38281 tacggtttcc gtttgccatc tgctttagac aatcggccac ttcggttaga agagtttgaa
    38341 aaacatgtca atcagatcat ttatgtctct gcgacgccag gaccatatga acatgaacaa
    38401 acagataccg ttatccaaca aatcatccgt ccgacagggc tgcttgaccc agtgatcgaa
    38461 gttcgtccaa tcatgggaca aatcgatgat ctagtaggcg aaataaatga acgagtcgaa
    38521 aaagaccagc gggtgtttgt aacgacattg acgaaaaaaa tggcagaaga cctcacagat
    38581 tatttcaaag aattagggat caaagtcaaa tatttgcata gtgatatcaa aacgctggaa
    38641 cgaacagaaa tcattcgaga tcttcgatta ggtgaatttg atgtgttagt tggaatcaat
    38701 ttgcttcgtg aaggattaga cgtacctgaa gtttcccttg ttgctatctt ggatgcggat
    38761 aaagaaggct ttttacgtag tgaacgttcc ttagtccaaa cgattggacg agctgctcgg
    38821 aatgaagaag gtaaagtaat catgtacgca gacaaagtaa cagattctat gcgtctagcg
    38881 atggatgaaa cgtcaagacg tcgtacgatt cagcagaagt acaatgaaga acatgggatc
    38941 gttccaaaaa cgatcattaa agaaatccga gatttgattt ctattacgaa agaatcggaa
    39001 gatgatacaa aagaagcggt acaagtctcg tacgaagaaa tgacgaagga agaaaaagac
    39061 acgttattga tgaaattaga aaaagaaatg aaagatgcgg caaaagcttt ggattttgaa
    39121 acagccgcga atgtgcgaga tatgatttta gaattaaaag cttcaaagta ggaggacaac
    39181 atggctaacg ataaaattac gattcatggt gcacgtgccc ataacttgaa aaatatagat
    39241 gtaacgattc cacgagataa attcgtggtc gttacaggtt tgtccggttc tggaaaaagt
    39301 tccctagcat ttgatacgct gtacgctgaa gggcagcgga gatacgtaga gagcctttct
    39361 gcttatgctc gtcagttttt aggacaaatg gataagccgg acgtagatag catcgacgga
    39421 ttgagtccgg cgatttctat tgatcagaaa acaacaagta aaaatcctcg gtcgacagta
    39481 ggtactgtga cggaaatcaa tgactattta cgcttgttgt ttgcacgtgt aggacatccg
    39541 atttgtccca acgatcatat cgagattacc agtcaatcag tagatcaaat ggcagatcaa
    39601 gtaatggctt tgcctgagcg cacaaagatc caagtgttgg caccgatcgt ggtaaagaaa
    39661 aaaggacaac ataaaaaaat attcgaacga atccaaaaag agggatacgt ccgcgttcga
    39721 gtcgatggtg agatctatga tttaagtgaa gtgcctgagc tggagaaaaa caaaaaacat
    39781 gatatcgcga tcgtcatcga ccggatcatt gtgaaagaag ggattcgttc tcgcctattt
    39841 gattctttag aggcggccct gcgattagca gacggctatg ctctggttga cgtgatcggt
    39901 gaagaagaaa tgttgtttag cgaacactat gcctgtcctt attgcggatt tactgttggc
    39961 gaactagaac caagactgtt ctcattcaat gcgccttttg gagcttgtcc agactgtgat
    40021 ggtttaggtg tcaaattaga agtcgatcaa gatttagtga ttccagatag aacgaagaca
    40081 cttagtcaag gtgctatcgt tccatggaat ccaatcagct ctcagtatta tcctcaaatg
    40141 ttagcgcagg cttgtgaaag tttcggtatc gatttagata caccattcga agaattaccg
    40201 gaagagcatc aggagatcat cttgaatggg acagatgatc ttttccattt ccattatgaa
    40261 aatgattttg gtggcgttcg cgatgtagaa acgactttcg aaggggtatt gaagaatatc
    40321 gagcgtcgtt atcatgatac gaacagtgat ttcacgcgtg agcaaatgcg tctttatatg
    40381 acagaattaa cttgtcagac atgtaaaggt tatcgattga atccccaagc tctatctgtt
    40441 caaatcaatg gaacgaatat cgggcaaaca aatgagttgt ctattcaaaa agcagtagat
    40501 ttcttctcaa acttaacgtt atctgaacaa gagaaagtaa ttgccaaacc gattttaaaa
    40561 gaagtaaatg acagactttc attcttggaa aatgtgggac tagactatct aactttgagt
    40621 cgcgcttccg gaacattatc tgggggagaa gcgcagcgaa tcagattggc tacacaaatt
    40681 ggatcgaatc tttctggagt tttgtatatt ttagatgaac catcgatcgg attgcatcaa
    40741 cgcgacaacg atcgactgat tggctcatta aagaaaatgc gtgatcttgg caacaccctc
    40801 gttgtagtcg aacatgatga agatacgatg tgggcagcag attatttgat tgacgtcgga
    40861 cctggggccg gagaacaagg cggacaaatc gtggctgcag gcactcctga agaagtagct
    40921 aatgatgaaa attctttgac agggaaatat ctttcaggga aaaaatcaat ccctgtacca
    40981 aaagaacgcc gtaaaggtaa tgggaaagca atcaagatca ctggtgcttc agaaaacaat
    41041 ctgaaaaata ttagtgttga atttccatta ggagaatttg tagcagtcac aggtgtgtct
    41101 ggttctggaa agagtacatt ggtcaatagt atcttaaaga aatcgttagc gcaaaaatta
    41161 aataagaatt ctgctaagcc aggtaaattc aagacaattt ccggctacga aagtatcgaa
    41221 aagatcatcg atatcgatca aagcccaatc ggccggacgc cgagaagtaa tccagcgact
    41281 tatacaagtg tatttgatga tatccgtggg ttatttgctc aaacgaacga ggcaaaaatg
    41341 cggggttata agaaagggcg ttttagtttc aacgtaaaag gcggtcgttg tgaagcttgt
    41401 cgcggggatg gaattattaa gatcgaaatg cactttttgc ctgatgtcta tgttccttgt
    41461 gaagtatgtc atggtaaacg atataattct gaaacattag aagtgcatta caaaggaaaa
    41521 agcattgctg atattttgga aatgacagta gaggatgctg tagaattctt caagcacatt
    41581 ccaaagattc atcgcaaact gcaaacgatt gttgatgttg gcttaggtta tgtgacgatg
    41641 gggcaaccag caacgacatt gtccggtggt gaggcacaac ggatgaaact tgccagtgaa
    41701 ttgcacaaaa tctctaatgg aaagaatttc tatatactag atgaaccaac gacaggactt
    41761 catagcgatg acatcgcccg cttgttgcat gtattacaaa gattagtaga tgctggtaac
    41821 acagttttag tgattgaaca caatctagat gtaatcaaaa cagcagatta tatcattgat
    41881 ttaggaccag aaggtggaga aggtggagga acgatccttg cgactggaac accagaagaa
    41941 atcattaacg taaaagaaag ttatacaggt cactatttga aaaaaataat ggtataaaga
    42001 ggatagatag ctgagatagt taaaaaaaca taaaaaaatg aatttttgct tgttttatta
    42061 atatcagtgt tctagaatca ttacatggta gttctttcta cactgattgg tatagataaa
    42121 aacttcctat aagttcaatc cgttcaaaaa agaattgagc aatcggagga ctttcctttg
    42181 attgcttttt tctttatggt atgatattcc ttgtgtagtt ggaaaagaaa aacaagagat
    42241 tatgagtaag tgagagataa tgatgaacag tattgaaaat gaagaattta tccctatatt
    42301 attaggaagc gacatgaacg tttatggtat ggcacgttct tttaatgaag catacggcaa
    42361 aatctgccaa gcatatgctt cggatcagtt agcccctacc cgctacagta aaattgtcaa
    42421 tgtagaagtt attccaggat ttgataaaga ccctgtattt atcgagacga tgcttcgttt
    42481 ggcaaaagaa agatattctg ataagagtaa aaaatatctt ttgattgctt gtggtgatgg
    42541 atatgcagaa ttgatctccc aacataaaca agagctgtca gaatatttta tctgtccata
    42601 tattgattat tctttatttg aaaggctgat caataaagta agcttttatg aagtttgtga
    42661 agaatatgat cttccgtatc ctaaaacatt gatcgtcaga gaagaaatgt tagtaaatgg
    42721 acatttggaa caggaattgc cttttgaatt cccagttgct ttgaaacctg caaacagtgt
    42781 ggagtatctt tctgtccaat ttgaaggaag aaaaaaagct tttattttag agactagaga
    42841 agagttcgat ttaattttag gccggatata cgaagccggt tataaaagtg aaatgattgt
    42901 acaagatttt atcccgggtg atgatagtaa tatgcgtgtg ctaaatgcgt atgtagatga
    42961 agatcatcaa gttcggatga tgtgccttgg tcatccacta ttagaagatc ctacaccagc
    43021 atcgattggt aattacgttg tgatcatgcc agattataac gaaaaaatct atcaaaccat
    43081 taaggcattt ttggaaaaaa tcgaatatac aggctttgcc aatttcgata tgaagtatga
    43141 tcctagagat ggagaatata aattatttga aatcaatttg agacaaggtc gtagcagttt
    43201 ctttgtgaca ttgaacggct tgaatctggc tcgttttgtc acagaagatc gagtgttcaa
    43261 caaacccttt gttgaaacga cctatggaac gaatcaatca gataaagcaa gattatggat
    43321 gggtgtgccc aaaaaaatct ttttagaata tgctcgggaa aatgaagaca aaaaactagc
    43381 tgagcaaatg atcaaagaaa atcgttacgg tactactgta ttttatgaaa aagaccgatc
    43441 aatcaagcgc tggttgttga tgaaatatat gttccataac tatattccaa gattcaaaaa
    43501 gtattttcat gtgaaagaag gctagttcat ggagaatttt ttcagtattt taggcggaat
    43561 gggcacgatg gcgacagaaa gttttgttcg cttgatcaac caccgaacga aagctacaaa
    43621 agatcaagaa tatttgaatt atgtcttatt caatcatgca acagttcctg atcgtacagc
    43681 ttatatttta gatcgatcag aagaaaatcc aatgccgttt ctgctggatg atattgagaa
    43741 acaaaattta ttgcgaccaa acttcattgt gttgacttgt aacaccgcac actacttttt
    43801 tgaagagtta caagcggcaa cggatattcc tatcttgcat atgccaagag aagcagcaaa
    43861 tgaacttgtc cgtcagcata cgacagggag agtagcgatt ctaggaacag aaggaagtat
    43921 gaaagctgga atctatgaac gcgaagtcaa aaatcttggt ttcgaaacag tgattcctga
    43981 tactgcgctt caagaaaaaa tcaattacct gatctatcat gagatcaagg aatcggatta
    44041 tttgaatcaa gagctctatt acgaaatctt agaggaagcg gtagagcgcc taaactgtga
    44101 aaaagtcatc ctaggttgca cagaattatc tttgatgcat gaatttgctg aagataatca
    44161 ttacccagtg atcgatgctc aatctatttt agcggatcga acgatcgaac gtgcattagc
    44221 tgaaagaagc gaggcattgg atacagcatc ggaaaagtaa aaaaattgga tttggggcaa
    44281 atatcctaaa agatatttgg tccaaatctt tttgtaaaag aaaaagaaaa ctggtaatga
    44341 tacgcccggg atgatataat tttattatgc acaatgtgag aggggttcgt aaatatgaca
    44401 gataatttgc aattagtcat cataacaggt atgagtggtg cagggaaaac agtagctatc
    44461 caaagttttg aagatatggg ctatttttgt atcgataata tgccgcctcg attgattcca
    44521 aaattctggg aattgatcaa agaatcaggt aaagtcacaa aaatggcttt aggagtggat
    44581 ttgcgttctc gttccttctt tgaagaaatc caaaacatgc tgatcgaaat cgaaaatacg
    44641 aacttcatcg acacttctat cttattttta gatgcttcag acgaagaact agtggctcgc
    44701 tacaaagaaa caagacgagc acatccaatg gcgatggacg gtttgatcac tgaagggata
    44761 cgtaaagaac gggcgatact agacgacttg aaagcccaag cttccgtgat cattgatacg
    44821 acaacattga caccacgaca attacgtgaa aaaatcaatg agtcattcaa atcaaaggga
    44881 gaccaaggat ttcggatcga atttgtatca tttggtttta aatacgggtt gccgatcgat
    44941 tcggatattg tgatggatgt gcgttttttg cccaaccctc attatatccc ggaactaaga
    45001 ccattgaccg gaaaagatca gccagtctat gattatgtga tgtcctttga tgaaacgcaa
    45061 tcattctacc ataaattttt ggatcttctg gaaactgtat tgcctggata cattaaagaa
    45121 ggaaaaagca gcttgacagt tgcaatcggc tgtacaggcg gtcaacaccg ttctgtggca
    45181 ttgactgaac gtttggcaaa tgctttaggg aaaaactata aagtcaatgt gacacatcgg
    45241 gacaaggata aaaggaaaga gacggtgaac cggtcgtgaa aatgaaaacc taccggataa
    45301 gaaaaccaaa gatcgtagtg gtcggcggtg gcactggtct gccagttatc ctgaaaagtt
    45361 tacgaaatca aggagcagat atcacagcag tagtgactgt tgcagatgat ggtggaagca
    45421 gcggtgcaat cagagaatcg atcgctatgg ctcctcctgg tgacttgaga aatgtcttag
    45481 tggcgttatc ggatatgcca caattctatg aagatatttt ccaataccgt ttcaaaaagg
    45541 aagatcagtt tttagccaat catacgatcg gtaatctgat cattgccgca gtttccgaga
    45601 tgcggggaag cacttatgag gcgattcaat tattagcaaa aatgatgcat gtagatggac
    45661 atgtttatcc ttcttcagaa actcctttga ccttgcatgc tgtttttaaa gacggaacga
    45721 ctgcagtagg agaatctaaa atcgcgatca accgtaaaac cattgaccgt gtgtatgtct
    45781 gcaacacaaa tgataaaagt gaagccaaag cggctcgaaa agtcgtttca gcaattatgg
    45841 acgccgacat ggtcgttttg ggacccggaa gtttattcac cagtatcctt ccaaatttag
    45901 tgataccaga aatcggcgaa gcaatgctca agacaactgc tgaaaccgtc tatatctgta
    45961 atatcatgac ccaaaaaggg gaaacggaac atttcacaga tgccgatcat atacgtgttt
    46021 tacacaagca tttgaatcag ccttttatcg atacagtatt ggtcaatacg gaaaaagtgc
    46081 ctgaagatta tatggaccca gagatctatg atgagtatct agtacaagtc gcacacgact
    46141 tcaacggatt gagaaatgaa ggatgtcgag tgatttccac cgacttttta gaattgaaag
    46201 atggtggtgt cttccacgat ggagacaaag tagtagaaga attgttccgt ttagttttcg
    46261 gttcaaaata ttaagcaatt tattgttgga gaaaggagga gaataggatg tcatttgctg
    46321 cagatgtgaa gaaagaacta actggtttgg aagttcatcg agaacatgcg aaagcggaat
    46381 tagctgcttt gctgcgaatg aacggctctt taggtattgt gaaccagcag tttattttga
    46441 acgtccaaac agaaaatgct gcaattgcca gacgtatcta ttctcttttg aaagatcatt
    46501 atcaggtacg aagtgagcta ctcgttcgta aaaaaatgaa attaaaaaag aacaatgtct
    46561 atatcgtccg gttgaagcaa ggcacaaaag atgtgttgat ggatcttgat atcatggatg
    46621 gtatgatgtt caatggacac gtttcggacg agattatggg aaattcgcaa aaaatgcgtt
    46681 cttacttacg tggtgctttt atggcctcag gttcagtcaa taatccggaa acgagtcgtt
    46741 atcatttgga aatattttct atttatgaac agcataacca agacatttgc gatatgttaa
    46801 attattacgg tctacatgct cgaacgcttg aacggcgcaa tggctatatt tcgtatttaa
    46861 aaggagcaga aaaaattgct gatttcttaa cattgattgg tgcaaccaac tcaatgctcc
    46921 gatttgaaga tgttcgaatc gtacgggata tgagaaattc ggtcaatcgt ctagtcaatt
    46981 gtgagacagc aaatctgaac aagacgattg atgcggcttc taaacaaatc gaaaacatcg
    47041 aattgatcga agcaaaggtc ggtctacatg cccttcctga aaaacttcag gaaatcgcag
    47101 aacttcggct tcaacatcca gaggtcagct tgaaagaatt aggggaaatg attccctcag
    47161 gtgcaatatc aaaatcggga attaatcacc gaattcgcaa aataaatgaa tttgcggaaa
    47221 aactgcgtga agaagcagtt taatccaaag aaaaaactga tacatgaagc aggagatgcg
    47281 aatgtatcag ttttttttaa aagctatact tgcttttctt ccgtcaaact ataattccaa
    47341 ctttactgga gtaacataat gttgaaaggc ttctattgtt ttttaataca atcgtataaa
    47401 aagcggggag acaactgaat gaagatttta gttttataca taaacttttg cttaatatag
    47461 aagtcctgat atagatagga tcaagtattt atctataact ggagattaat taaaaactgg
    47521 aaggaactgc tcgatcaacc ttgagaactg tttttcttac ttctggtcaa gattctcgtg
    47581 gttggtggat aaggatggtt gataagttca ttggattagc tagtcgaaaa caaccaggtg
    47641 taattaatgt agaagtttta acaaggggat ggagtgattt attcagtgat aatcataagt
    47701 tcataaatag taattgggat gtatggttgg tatacgttat acctgaactt gaactatcaa
    47761 gatttggttt agaatttgag aatcgatgtt tataaaggga gggcaaaaaa tgtgcttttt
    47821 tacaactttt actatcatta tgattgcagt actttttatt tttctagata tagcaaagcg
    47881 aaatacagca tttttattat atcgggtttt acttcgagca ggtctcataa catttatatc
    47941 gatcgttgga ttttttctat tcacagttat tgtatttatt tggagaacta cagcaccacc
    48001 attgccagag attacatatg gagaatttcc gtttagtctt gaatatgaaa tagatgagga
    48061 aagaattttg attgaagata cggtgattgc agaatttata ggaagtttta gagggaatgt
    48121 gacaactcaa cctagacgaa gttggagtat aaaactttta aataatagtg gagagttgaa
    48181 agcatttcat tatgaattct taataataga aagtgaagat gtaacaatca ctttaagtag
    48241 tggctctgct ggatatttta tgaatgatgg tggaagcaag ccacagctca cagtccgtga
    48301 tcgtattgca ggttttatgc atttttctca ctatgactta gaagaagctc gtaaaatact
    48361 tgcagaatat gggatcacat taatcaattg ggaatatgcg ccaccgataa gaaatttttt
    48421 caatgattat tattagtctt ataaaaaaca gaatgtaaaa tttatgtgcg cgagtattta
    48481 gttttccatt ttttgtttga tagatatata gcattgttga agcatgggat agtggtgcca
    48541 attttatcaa tgaggttggt caggcagtta acataattgg tgatgctata aatccattta
    48601 attggaattg gaggaattag gtgtggaaac agtgagatta tggatattca tgaaaaattt
    48661 tcgttcggct ttgagatatg ttattctgga atcaaaatca ggttattatc tgattgacac
    48721 agatcactat actttttggg gaggctacct ttttccgatt tttaattgga taatccccca
    48781 taaggttatg aaaattaact tgcctgcaga agaaataaat agattaatac tcaacaaaga
    48841 tttacaaagg aaaagagaga aagaacattc taatgttgct tggataggtc cagcatttgc
    48901 agtagtgact tcttcaattg tcggtcctat gctagagagt atttatagtg atttttctat
    48961 tggaatacgt tatttatttg ttactttaag tatagctgtt cttttgtgca taaagggtta
    49021 tgttagcaag aagaataaag atattttaac actaatcggt gtaaacaatt tatctagtac
    49081 taaaattata ctgtttcctg aatctataaa agaaggatca aggttgttga taatttttat
    49141 aatgcttata ctgttttctg ttttatttgg ggtgatgttt ata
//
LOCUS       DASJPC010000009        45590 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJPC010000009 DASJPC010000000
VERSION     DASJPC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.009"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(17..334)
                     /locus_tag="U4A13_000502"
     CDS             complement(17..334)
                     /locus_tag="U4A13_000502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778828.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(361..567)
                     /locus_tag="U4A13_000503"
     CDS             complement(361..567)
                     /locus_tag="U4A13_000503"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778829.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(548..730)
                     /locus_tag="U4A13_000504"
     CDS             complement(548..730)
                     /locus_tag="U4A13_000504"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778830.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(744..1094)
                     /locus_tag="U4A13_000505"
     CDS             complement(744..1094)
                     /locus_tag="U4A13_000505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778831.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1120..1422)
                     /locus_tag="U4A13_000506"
     CDS             complement(1120..1422)
                     /locus_tag="U4A13_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778832.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(1425..1658)
                     /locus_tag="U4A13_000507"
     CDS             complement(1425..1658)
                     /locus_tag="U4A13_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778833.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(2644..2966)
                     /locus_tag="U4A13_000508"
                     /pseudo
     CDS             complement(2644..2966)
                     /locus_tag="U4A13_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3089..4204)
                     /locus_tag="U4A13_000509"
     CDS             complement(3089..4204)
                     /locus_tag="U4A13_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1778834.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(4210..4608)
                     /locus_tag="U4A13_000510"
     CDS             complement(4210..4608)
                     /locus_tag="U4A13_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1778835.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(5637..6023)
                     /locus_tag="U4A13_000511"
     CDS             complement(5637..6023)
                     /locus_tag="U4A13_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1778836.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6020..6541)
                     /locus_tag="U4A13_000512"
     CDS             complement(6020..6541)
                     /locus_tag="U4A13_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1778837.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(6543..7577)
                     /gene="sppA"
                     /locus_tag="U4A13_000513"
     CDS             complement(6543..7577)
                     /gene="sppA"
                     /locus_tag="U4A13_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1778838.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(7610..9025)
                     /locus_tag="U4A13_000514"
     CDS             complement(7610..9025)
                     /locus_tag="U4A13_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEN1778839.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(8994..11147)
                     /locus_tag="U4A13_000515"
     CDS             complement(8994..11147)
                     /locus_tag="U4A13_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEN1778840.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            11405..11641
                     /locus_tag="U4A13_000516"
     CDS             11405..11641
                     /locus_tag="U4A13_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEN1778841.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            11660..11875
                     /locus_tag="U4A13_000517"
     CDS             11660..11875
                     /locus_tag="U4A13_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEN1778842.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(12578..13330)
                     /locus_tag="U4A13_000518"
     CDS             complement(12578..13330)
                     /locus_tag="U4A13_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1778843.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(13345..14592)
                     /locus_tag="U4A13_000519"
     CDS             complement(13345..14592)
                     /locus_tag="U4A13_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1778844.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(14668..14814)
                     /locus_tag="U4A13_000520"
     CDS             complement(14668..14814)
                     /locus_tag="U4A13_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEN1778845.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(14918..15229)
                     /locus_tag="U4A13_000521"
     CDS             complement(14918..15229)
                     /locus_tag="U4A13_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1778846.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(15231..15428)
                     /locus_tag="U4A13_000522"
     CDS             complement(15231..15428)
                     /locus_tag="U4A13_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN1778847.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(15822..17549)
                     /locus_tag="U4A13_000523"
     CDS             complement(15822..17549)
                     /locus_tag="U4A13_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN1778848.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(17542..18735)
                     /locus_tag="U4A13_000524"
     CDS             complement(17542..18735)
                     /locus_tag="U4A13_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1778849.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(18922..19566)
                     /locus_tag="U4A13_000525"
     CDS             complement(18922..19566)
                     /locus_tag="U4A13_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1778850.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(19660..19794)
                     /locus_tag="U4A13_000526"
     CDS             complement(19660..19794)
                     /locus_tag="U4A13_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN1778851.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(19796..21928)
                     /gene="feoB"
                     /locus_tag="U4A13_000527"
     CDS             complement(19796..21928)
                     /gene="feoB"
                     /locus_tag="U4A13_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN1778852.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(21925..22398)
                     /locus_tag="U4A13_000528"
     CDS             complement(21925..22398)
                     /locus_tag="U4A13_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN1778853.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            22799..23023
                     /gene="nrdH"
                     /locus_tag="U4A13_000529"
     CDS             22799..23023
                     /gene="nrdH"
                     /locus_tag="U4A13_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN1778854.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            23182..25341
                     /gene="nrdE"
                     /locus_tag="U4A13_000530"
     CDS             23182..25341
                     /gene="nrdE"
                     /locus_tag="U4A13_000530"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN1778855.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            25391..26356
                     /gene="nrdF"
                     /locus_tag="U4A13_000531"
     CDS             25391..26356
                     /gene="nrdF"
                     /locus_tag="U4A13_000531"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN1778856.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(26445..27584)
                     /locus_tag="U4A13_000532"
     CDS             complement(26445..27584)
                     /locus_tag="U4A13_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1778857.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(27893..28606)
                     /locus_tag="U4A13_000533"
     CDS             complement(27893..28606)
                     /locus_tag="U4A13_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN1778858.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(28860..29561)
                     /gene="nagB"
                     /locus_tag="U4A13_000534"
     CDS             complement(28860..29561)
                     /gene="nagB"
                     /locus_tag="U4A13_000534"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN1778859.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            29795..31327
                     /locus_tag="U4A13_000535"
     CDS             29795..31327
                     /locus_tag="U4A13_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1778860.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(31518..32720)
                     /locus_tag="U4A13_000536"
     CDS             complement(31518..32720)
                     /locus_tag="U4A13_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1778861.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(32802..33491)
                     /locus_tag="U4A13_000537"
     CDS             complement(32802..33491)
                     /locus_tag="U4A13_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1778862.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(33597..33965)
                     /gene="rplL"
                     /locus_tag="U4A13_000538"
     CDS             complement(33597..33965)
                     /gene="rplL"
                     /locus_tag="U4A13_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN1778863.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(34018..34521)
                     /locus_tag="U4A13_000539"
     CDS             complement(34018..34521)
                     /locus_tag="U4A13_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN1778864.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(34725..34852)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(34931..35620)
                     /gene="rplA"
                     /locus_tag="U4A13_000540"
     CDS             complement(34931..35620)
                     /gene="rplA"
                     /locus_tag="U4A13_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN1778865.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(35724..36146)
                     /gene="rplK"
                     /locus_tag="U4A13_000541"
     CDS             complement(35724..36146)
                     /gene="rplK"
                     /locus_tag="U4A13_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN1778866.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            36489..37157
                     /gene="sdaAB"
                     /locus_tag="U4A13_000542"
     CDS             36489..37157
                     /gene="sdaAB"
                     /locus_tag="U4A13_000542"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN1778867.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            37170..38042
                     /gene="sdaAA"
                     /locus_tag="U4A13_000543"
     CDS             37170..38042
                     /gene="sdaAA"
                     /locus_tag="U4A13_000543"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN1778868.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(38133..39152)
                     /locus_tag="U4A13_000544"
     CDS             complement(38133..39152)
                     /locus_tag="U4A13_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1778869.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(39325..40230)
                     /locus_tag="U4A13_000545"
     CDS             complement(39325..40230)
                     /locus_tag="U4A13_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1778870.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(40289..40834)
                     /gene="nusG"
                     /locus_tag="U4A13_000546"
     CDS             complement(40289..40834)
                     /gene="nusG"
                     /locus_tag="U4A13_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN1778871.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(40935..41105)
                     /gene="secE"
                     /locus_tag="U4A13_000547"
     CDS             complement(40935..41105)
                     /gene="secE"
                     /locus_tag="U4A13_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN1778872.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(41129..41281)
                     /gene="rpmG"
                     /locus_tag="U4A13_000548"
     CDS             complement(41129..41281)
                     /gene="rpmG"
                     /locus_tag="U4A13_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1778873.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            41443..42087
                     /locus_tag="U4A13_000549"
     CDS             41443..42087
                     /locus_tag="U4A13_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1778874.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(42143..42646)
                     /locus_tag="U4A13_000550"
     CDS             complement(42143..42646)
                     /locus_tag="U4A13_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1778875.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(42659..42705)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            43243..44208
                     /locus_tag="U4A13_000551"
     CDS             43243..44208
                     /locus_tag="U4A13_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN1778876.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(44307..45479)
                     /locus_tag="U4A13_000552"
     CDS             complement(44307..45479)
                     /locus_tag="U4A13_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778877.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(45466..>45590)
                     /locus_tag="U4A13_000553"
     CDS             complement(45466..>45590)
                     /locus_tag="U4A13_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778878.1"
                     /translation="LYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        14020 a         9067 c         7462 g        15041 t
ORIGIN      
        1 aacatttttg aagaaactat ttttctaatt tctcaatttc ttccaaggga ttttcgctaa
       61 tgacccatac aggcacagat tgctctcgag gctccccttc ttctaaccac caacctgcat
      121 aatgaacttc ttcctcctca gcattaccat gataccaacc aacaaaggat tcatctacaa
      181 cgaatgaaat ttcttcttct ggatcaaata attcattttt ccatgcttca attgcttgtt
      241 ctttggtgta cttttgtttg ttaaatacac cacaaatttc gtcatctaca taaaaaattt
      301 caaaatcaaa attactcgtt ccttttactg gcatcttaac ttttcctcct ttgactcact
      361 tcaatagata taccactcat gttcattcca ttgtttacta gttaattgga gttttatctt
      421 agggctttgt gcactctttc caaaaacttg agcgtcgttt ttttcttcta accaaaaaag
      481 gtaatttctt acattcggta ctaaatgttt ttcctgcatt tcttcatgaa aaacatataa
      541 ttctgcttca tttttcaacc aattcattat tcattccctc attcacttct tcataccgcc
      601 attcagatag tgagatttct ttttgtttct ccacttgtcc tataaaaatc gctgtattca
      661 acaagctgat cacacttaat aataaagcaa tgaaactaat ggttacactt actctttcac
      721 attttgtcat tttgtttcct tctttactcg tttgttattt cttgtaatag gtctaataac
      781 tccttcaccg aaatttgcaa atggttcgca aaaatttcaa taaaagcact cgcacacatt
      841 accagacctt caactgatat atcactgcga caataaaatt ctggagaaac actgatttct
      901 gattctttct tacaattgaa cgaaaaaact atttttgaat caaattccgg taattcttcc
      961 acaactatgt tttgggattc taccaagaaa gggagtgcct gttctaatgc ttcttctggt
     1021 gtttgagcag atagaataat tgcttcttgt tcttttattt cattgttctc aaatacttta
     1081 taactttttt tcattgttag ttcctctttc attcctctct caattagcta agccgttact
     1141 ggtacgccat tctctaaatt tagtatcgct aatccttaat gctcgtcgaa tatcaatcat
     1201 tcgataacct attgcacgaa gttctttata tcttttagca gttagcgtct ctaacgtcat
     1261 ttcaggcaat gggttcgaat caaaaataga attcgaaccg attatttggg ccaattgttg
     1321 tacttgtaac agctcttctg aacttaattc agtgattgct cgattgccta tcattgcata
     1381 cattttctgt cgaatatact tcttttcttg tttagacaac atttctatcc actcctattt
     1441 tcattatcat ttgttacctc cacttcagta aatccacaaa taaatgaatt catattccaa
     1501 gtaccattcc cttcaactac aacttttctc ctgtcctctt tcggatattc aagaatcaga
     1561 ccgtttacca atatgttttt cactactact aatttgtttg tataccgtgg aattgctgtt
     1621 ccgatatact tcactttatt tcctacgcta attgtcattt cctcactcct attctttttt
     1681 atccaaaact ttttcctgtc ctattcctgc caacgaatat acttcactgc atgcttaaac
     1741 aaaacaaaat gttgccctgt tttatttccg tcttttactt gttccacata cagttcatat
     1801 ttggtcacac cttttaaaac gccagatact ttttttcatc cattgtcaca attagttttt
     1861 tcatcaatcg attgatactg cactgctcat ttttcttgtt catatagtat tcctttcttt
     1921 ttcaaaacta gcctcttttg tttactagtt ttcatcaatc atcaacgatt taacagattt
     1981 tttctttcgc agggtttcta gcgtcagcaa ccccaaactc taaaaaagtg cctttgctcc
     2041 cttgaaaaac gagcggacga ttgtactcgt catcatatgt ttttcatact acctgatcca
     2101 cgcaaataac ctttttttgt tctgtgccac acccatccat acgtatcgct tgagattcac
     2161 catacttttt taggtggttt atccttccta agtccctgtt agacacgctg attaacttag
     2221 aaagtcctgt ttgtatccaa ttttcaaaga tctcccaaaa gaaataagta ggtttacgtg
     2281 tatcgcttct gttcaaagta cctgtttgta cttgagtatt tctactcccc tcgttcaagg
     2341 gattccttcc accagctagc ttttcctttc atcatcaaat gttcctgaaa agcatttacg
     2401 tttcgtgtcc gtgtgcttac ttattctttt ttcgatagcg attatccgct cgctactcgc
     2461 tattttatac tttgtttgtt agctcaaata aaaactaaca aacaaaaaag gctagtttct
     2521 ttccgtttta tttttttaac tgtcgataaa tagttgttag tttaattcca agttctaaaa
     2581 ccaatacgcc caacaaaacc acaggaaaaa caattggggc aaacaagtaa aatcggatag
     2641 ttttcatgtc ttccctcttt ctttttggtg tcgctgtggt cgttcttgcc gttcaatact
     2701 tcgatacatt ttttattcat tatttgctcg ttttgttttc ctctggactg cttttcttgc
     2761 tttttgaata ctttatacgc ttttcgtgcc tgaattaata ggtcttgtcg gcgtttcatt
     2821 cgtgagaaaa caatatttga aaccatcaaa tcctggttcg aacaaccacg attcgcaata
     2881 attttcccat caaaaaaaat agttttcact tgagaaaaaa acagttggaa atcttgtttt
     2941 gttgctaatt tttttgtcaa aatcactgtg cttttccctg ttgaatctac aatgattctt
     3001 cgaacaatcc gattcgataa cttccaacgt tgccgattaa acaatgttac ttcacaacaa
     3061 atatgacgta agtgattagg ggtcattttc atgtgtccaa ttcctccaac caattatgca
     3121 tcgaaatttt gggcaattgt gacagcccat aaatctgcgt ttccaggtct tcataactac
     3181 aagaacgctt ggcttgaagt aatgaatatt ctgcttgatc tgatagtttt gttgccattg
     3241 gatctagatt caattctctt acacaacatt cccaaaaatt caatagcgct ctaaaataaa
     3301 atccttttcg attctgaatt gcttttcctt catgacgagc aattttttca ttttgaatac
     3361 accgttgata cgttttgctt aaatcttctg caaatgtttc ccctaaaatt cgatactcgc
     3421 ttccatcaga taaaccatgt gtttgattat actttcgttg ccgcttttct ataattcgtt
     3481 tactgtcgta tatcatgttt agctgctctt tcgcttcacg ccattcagct gaactgcgaa
     3541 caatccatgt caaaacatct ttcggaacaa acgtactttc gctgtaatga gcaataaact
     3601 ctttttgcca atcatcagcc atttcaacag gactagtcgt atcgattttt gtatctagat
     3661 tttctgtatc tagattgtta gaagaattat taaataattg tgcagaattt tcacatggat
     3721 ttttttcgtc aaaatcatcg aaaatcgcta aattatcaac agattcctcc tcttcttttc
     3781 cttgtgcaga atctacactg gttgagtcaa caggacctaa tgaatacaca tcattttctg
     3841 tcgtatctaa ttctcctaga tacaaccgag ctttgtttct ttttcctgtt tgttgatcaa
     3901 atcccatatc ctctatttct agtagctcat attctttcaa ttcttttttg attgtattaa
     3961 cagaacgtct gcaacaattc aatgcattgg ctaattcttg ttccgtaaat tcaaaataaa
     4021 caaaaccttg actatctact tgatttcgtt gaatggctaa atctaagcgt gaccgaaaaa
     4081 ccatatacgc gatttttgct ttatcagaca atgagcgata tttttcactt gtaataaata
     4141 cctttggaaa ttgatagtat aattcattgt atttacgagt tgcggtaaat ctttttgttg
     4201 ccatcgttat tacacctcat cttgtccata tattaagaaa gaattttggt gttggtagac
     4261 acgaacatct tccattgtag caatcaattc ttttagataa attcgaccga attcaattgt
     4321 tagtaagtcg taatcttcta attcattttt gatttcttca atttcttctt cagacacatt
     4381 gagtaaaatg gctaattctt gattggtata agaaatatag acatgtttct tttcatcaat
     4441 ccgtttttca attaaggatt cttctaagta ctcattcagt aagctatagg cgtattttgc
     4501 ttgtgcagtc atcgcataat attttgacga ttttataaag aatagttttg gcatatgaaa
     4561 agttgcttgt gtcagcacat ctgttgcgcg aaatttgttt tttaccatat tttttcctcc
     4621 tagaaaaaca cgcaaaaaaa gctctgtata aatcgaatta cacagagttt cttcttgttg
     4681 atatttaatt tctaaaatac gtttcgggta aaaactaaac acttactaat atttagattg
     4741 tagaatcttc gccttcattt attcatttta aagagaaaaa gatcaagtga aatgtgccca
     4801 aaagtttgat tctattaatt tttaaacacc attcataata tatttcctat aatgtaaaaa
     4861 gcgtcgatac ttattaaagc atcgacgtta tttttatagt aaatttatca aaatttttgc
     4921 caacattttt tccgattttt ttccctattt ctatctttcc ttatctttct tagttaatat
     4981 tatttgatgt taatatatca ctactaatat taaaaaacat tgatttatca taggataaag
     5041 agtgatagtt taatacttaa agagacatat taattttatt taaaaattga cattaatgac
     5101 tcttaatcag cgggtcgcgg gttcgagccc ctcacggccc attgggtgcc aaacccacgg
     5161 aacaggtaaa tgttggtgcg aaagcatttc gaaaacattt acgctgttct ttttttattt
     5221 ttcaaaataa ctagggcttt ttatttttaa ctcaaagtcc actgtagccc tttggacttt
     5281 gagttaaaaa agaagcataa ttatgaacag taaagtttct aatataacaa taattcgaga
     5341 agctttgcaa aatcatgctg ccaatgaact ctctaaacat ataggattaa atctaagtac
     5401 tataaaaggt taaaaagtga agaatattta atagaaaaac taaatttact tgctgctttc
     5461 cccttttcca ttaatacaac aatcagagag tgtctttttt tcgaaaaaat tagaataaat
     5521 aaaagacacg tagttttttt atctacatgt cttccatcga ctacaagtta tcatttctca
     5581 aaacgtattt agcttttaat tgattaatcg tttcacgttg tctacacatt gcctaattaa
     5641 gattttctta ttagaagcat caatcccgca ataataccta aaactccagg aattacccac
     5701 aataatgtta aaagaaaaaa tggaataata gataaaatga gtaaactaat accatagacc
     5761 actgtttttt gattttttct tactaaaaaa cctattatga aaatcattat cgaaaaaact
     5821 agggtaattc ctccaaataa aacaacattc ccgcttagat tgactaacat atctatatcc
     5881 ttactggtta gttctgtagt atttaaagta gttgaatcaa tagaagatag aaacatacca
     5941 cccatcaatg ctaaaagact acataaaatc cctgttattc cactaattaa aatcaaaata
     6001 aactctgttt ttctcttatt catttaattc tctccagatt aagtgtttta gaattagaaa
     6061 ttttttgacc atagttaata gcttctaatg aacgaataac aaccactgac gtgttagaaa
     6121 aataatcccc aatatgttgt ttcttgggcg tgaaagcagc caccaaatac ccaatttgaa
     6181 atacaaaatt ttttaatata aacctacata tacactcacg aataaagata gtttgccaac
     6241 ttagctcttt ttcctcaaag ctcactactc ttatcccaaa aatcattttc ccaattgttt
     6301 gtcctgaatt cagcttagtc aaaagtacaa aataggttaa ataaatagct aatgacgcta
     6361 taccataaaa agtaataatg gatttgtcag attccagtcc catgattttg taaaagagac
     6421 ctatagtagc atgggtgatg atgctaatac aaattaaatc aataagaaaa gcaaaaaatc
     6481 gtaaccaaaa tcccgcaaat acataattag gcaaagtttc aatgccactc ttcttattca
     6541 ttttatagcc ctccatagta atacattggt tttggagtat cagaaaatcc aagtttttca
     6601 aaaactgaaa ttacacgatc tgatgtagtt gcctttagcc cttgccattc agcaatctta
     6661 ttacctaacc aagaagaagc aaagccagtt gtgtcatttt tatattctat aaccgtcgca
     6721 tcagttaatt tcttctcttt ttttagggaa tctaatgcat cttcagagta accaatttga
     6781 tccaccaacc cattttctac tgcttggtta ccatcataaa ttcttccatc cgcaagttct
     6841 ttcactgcat tagtatccat atttcttcct tttgctacaa cattgacaaa acgatcataa
     6901 gcactcatca caaattcctg tataacttta ttctcttcct ctgaaggttt gtgttgaggt
     6961 gacatcatat cttttaatgc accactttta tacgtcgcat cagttacgcc aagtttttct
     7021 aataagccag aataatttaa accagaaata atgacaccaa ttgatccagt tgttgtttct
     7081 tccgtagcaa atattttatc tgaaccagca gaaatgtagt accctccact tgccgctgta
     7141 tttttaaatg cagtatagat aggaatatct agctttttaa tttttgccat ttctttagca
     7201 atctcagcac tttcatatat tcctccccca ggtgaattta cttctaataa cactccctta
     7261 accgctttgt cttcttgaat tttctttagc tgtgttaaaa aattctgatg gttatattgt
     7321 tccctactaa aaagattgct ttcacctgta tctgcaataa ctccattgac ggatagtttg
     7381 acaatctttt tatttgatgc gccttcttct aacgtttctt ctgtgatttc gttagatccg
     7441 taaaaaatct tatttattcc tgttagggtt gtatttgatg cttcctcttt atcttttcct
     7501 ggaattagcg cgacgataat ggatagtaac aataatccgc acgctactcc cacagcaatc
     7561 catcttcttt tattcatata aactcctcct atattcttag taaaaataat tattttttat
     7621 ttagaaacac atcgacaatt tgttgccaat atgttttttc tcctacaatt aaagacaact
     7681 ctccagttaa tccataacga aacggaaacg attgagttgt ttttaacgtt cctttcactg
     7741 cataaaaaac acctttctca gtggcagtag aattttctga aatttcatct aaagttccgt
     7801 cgatcgtaat cggagcccca cctttttgat ccaatttaaa atgaaccttc atcccttctt
     7861 taatataggt actttcattc gcaggtaaaa gtgctgtaaa tttcattttt gatttttcgt
     7921 ggatcggata aatttctgca agcactgttc ctttggctag ttctgcttgt ccctttgttt
     7981 cttcatttaa atgaaccgtt ccagatacag gagcttttag tattcctttt gaaagttgat
     8041 cgtccaaact tttgataact gactggttct tctcttgttt ttcatttagc tctgtaactg
     8101 tttgttttac agttgttatt gcttgttcta gctcttgctt tcctttttcc gtttgactaa
     8161 ttctgtcatt ctcagaagta gttggtttaa ctaatttagc tttttctgac tgatactgtt
     8221 ctacttcttt ttttagttgc tcaatttgtt catttatgct tgctgaaatt gtcagtttca
     8281 cttgattttt ttgatcatca gaaacattat ttagttgttc ttgccaattc tcatagtttg
     8341 ccatcatttc ttttgaaaaa tcttttaatg attgattatt actccaagct acctgtactt
     8401 gttgccattc ttgtaattta ctatctgaac tctcaatttg tttttctagt tgtctttttg
     8461 tcttttcgta tacttctaat tgcttttgat catttaattc actttgcttc aaagcgtagc
     8521 ggagtgattc tttttctgac aacatgctct ttaattgatt actgtagcca aaactgtcat
     8581 ccgttgaaaa caaatttttt cctttactta tactttcaat aaatagctga gccatcttta
     8641 tttgttgagt aatacgttgg ttttcttgtt ctatctgagc cttttcattc tctattaaag
     8701 aacaatccaa tctaaccaac acatcgtcct tatgaacaaa ttgattttct tttaggaagt
     8761 tttccattat tttagcttct aatggcactt gtaacttctc tattttttcg gggttcaatt
     8821 gagccgttgt ccgaataaca acctcttttt tagcaaaggt taaaaacaat ccaataatac
     8881 ataaaaaaag aattacaggg tataacaccc aacggtagaa gctatgatgt tgtttagtat
     8941 agatactaga atgttcgaac caattattat tctgcataaa ttgtttccct ctcctagatt
     9001 tcccataatt tttgatagat accatcggca aaacgaagtt catcgtgcgt tcctttttct
     9061 accaaatgcc cttcatggag aactaaaatt tgatcacatg cttttgctat tggtaaatga
     9121 tgcgcaataa aaataatcgt tttttctttt aaattgagga gataattcat aattgcatgt
     9181 tctaacaagg tatctaatcc actagttgct tcatctaaaa ttaatatgtc cggatttttt
     9241 aataaagccc gtgctaaggc taatcgttgt cgttgccctc ctgaaagatt atttccacct
     9301 tcttccaaca atgtctcgta acgtaatggc tgttgattaa taaattcatt tagttggact
     9361 atctcacaaa cttctattat ttgttcgaaa cttggtgggc tatctaaacc gaatataaga
     9421 ttctcaatga ttgttccact aaagaaaaat gactcttgag gcaaatatgt cactcgatcg
     9481 cgtaatatct gaaaaggaat gtctaagtga ttaatgtttc cataatggat cgatccttct
     9541 gatgcttcat aaaaacgaac aaataacttt gcaagagttg attttccaga accactaact
     9601 ccaactatag ccgttttact gtgcgaagga attgtacatg aaatttcgtt caacgttggg
     9661 gccttcatgc tatacgagaa tgaaacgttt tttacagtga tatcttgttg aaataattgt
     9721 tcagaaagca tatattcaaa agactggttc gatttttctg aatcaatcgc gaaaatttca
     9781 tttaaacgat tatttgcaac ttgagccgtt tgcattttca cttgtaaatt aataatgttt
     9841 tgtaaaggat ctgtcaaaaa tactagtaaa gcattgtatg tgataagttg acctagacta
     9901 attctattat ccataacaaa ataagaacct agccataaaa ttaatgcact gctaattaat
     9961 tgaatcaaat gtttaattcc ttgttgaata ttatcaagcg taaccgtacg aaatgatttt
    10021 ttcattaatt taacaaattc atggtctata cggttatata ctttctcttc accgctatat
    10081 gctttaattg tttctattcc tttaagactt tctatgatac tagaatttac agctgcaccg
    10141 gctgccattt cttccctgtt cgatttttca tagcttttca cgaaagcgaa tattgcaact
    10201 acataaaaag gcaaagagca tagagtgatt aaaaacaacg tactattctg aatagctaac
    10261 gtaaccccaa caagcaacac cataccaata tctaaaaata ttgataacgt agcacttgct
    10321 aaagcatcaa taatcttact ggcatcaaga aaacgagaaa taatttcccc tgattttctc
    10381 gtagcaaaaa aattcattgg taaagttaac acatgtttga aatactgaag cataattgcc
    10441 atactcattc gctgtcctaa aataataaga agataactac gactatattc aaacaatacc
    10501 cgaaataaat agacagcgat caagccaatg gaaattatat ttaaagttga acgtgcttga
    10561 ttagggatga aaaaatcaag aataccttga aaataatatg agcttcctat gccaaaaaac
    10621 gtaatgaaaa tcgatgctaa aacgatgtgg aatactaatc ctttttgttt ccaaattata
    10681 ggcaaaaaag aacttaatcc agctactttt tctttaatag gaatataagt ttctctaggc
    10741 ataggtaata aaagaatacc tgtccattct ttttgaaacg cttcaatcgt ttttctattt
    10801 tttccattag cgggatctgc aatccatagt acgtttccct tcgttttata aattacaacg
    10861 tagtgcatat agctgttatc aaccaatata tgtgcaatta atggtaagat aagatctctt
    10921 tctttccaga tctcactatc tgcttgaata gccatacaat caaaagacaa tttttcaaat
    10981 gtctttttca atccaaatgc tgaagtaccc tctaaatcag tcccagatag gtcccgtaat
    11041 ttatgaatcg ggatatcagt tttaaaatat tttaaaatca tcgaaaggca agcaacacca
    11101 caatcttttt catcttgttg ctgcacaaaa ggaaatgatt ttatcactta tctaacacaa
    11161 ctccttctaa aaaattttta tagaaaaatt ctcaatatag ctttaataca gtgaaaatat
    11221 tgtctcataa ttccacaaaa actttgaaaa cagttcttga acactaactt tttcattcct
    11281 gaatcaaatt tattttggaa attttactat tcaagaataa acttgataag tttcaggaag
    11341 atttttactg ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata ttatttaaag
    11401 aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg cacaaactgt
    11461 tggtggaggc aataatgctt ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg
    11521 ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg gtgttgttgg
    11581 agggttatat ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata
    11641 aaaaaggaga ataagtagta tgcatattaa aaacacaaaa acaacattta tattatcttc
    11701 agaagagttg aaaaatattc aaggtgggag tgccgtggga gttttaggca ctactttttc
    11761 tggggcaaca gcaggagtaa aactttgttc agccggagga ccttatgcaa tagctgcttg
    11821 tggtgtaggt ggagctttat taggtgctgg attcagcatg tggaccggtg cgtaaacaat
    11881 taaaaaatat caaacctcaa gattggtttg tgtcgattac gaacataggt atgttcttta
    11941 tcttttcaaa agtttggaat ggacagaact ttttactaaa gcttcttgtg ctagttttta
    12001 taattatatt aaatttttat cttttattaa agtcagataa aaattctttg gaaatgtaat
    12061 ggtagagata tttttacaaa aggacttgca gcgctgtagt tggtgctgca ggaggaccta
    12121 tttcggccgt tacaggaggt attggtaatg tacttgtcgg atgtgcatta aagcctgttc
    12181 ctgcaagtta aatttataaa aacaggaaag taattatctc aaatcctatc aagtacaatc
    12241 ataggattgc acttgacagg acaaattttt taactaaaaa atgaaaaaca ctttctaaaa
    12301 attagtagaa tttgccaaat ctttcgcatc ttccaccata catttaattt taattcccct
    12361 ttatggtact taataaaaac aagaaaagaa ctattcattt ggcccttttt tggttccaga
    12421 ccatagaaat attcctccag taactaatat tgctgttatg cacgtaacta ataacagcta
    12481 taaaaattgg tacaacttta ataggattca gaaaaatttg cactagtgaa ggaatctatt
    12541 ttattagaaa aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact
    12601 tctccttaat tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat
    12661 atagtgaacg ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc
    12721 gactaattct tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt
    12781 cgtactgccc atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa
    12841 aataaatgtc atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt
    12901 gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa
    12961 tctcgtttcg atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc
    13021 ttctttttat tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt
    13081 gaacatcata ttttctgatt acttctgcta attctatacc gttaacttca tgatttaaat
    13141 caatatctaa gaaatatatt ccttgctttg gtcggaattg tttgaggtat tttttaactt
    13201 ctaacggact ttgtgttttc aatactatct taaatacttt atcgtgaaaa agaatgaaat
    13261 tatcaataat tcgttctaac tgatttaatt gtataatttg atcttcacat aatattattg
    13321 gataactcat aaatcctcct tccctcaaaa caacaaaata acttgagtag taaattgata
    13381 cggatcgttt tgatagttca caaacatatt gggattcttt ttattgattt cctctatact
    13441 agataaacct aacccttgat gtcccttttt tgttgttatt ccttttttca ttagagtatc
    13501 tatagagagt agtatctctc tacaactatt cgatatcgaa atttctatct gtttatcatc
    13561 tcggtaaata gctagagaca gttctctatc ttgactctct atagatgctt caattgcatt
    13621 atcgagaata attccaagca ccctaataca gtcaaatatt ggaacaggaa cttcaataat
    13681 agtacttgga cattcgaatt tacaacaaat cttattgtta tttgcttggt atagcttcgc
    13741 tatcaacaaa cttttcaaat attcattttt tatatttttt aaataacgat attcaaactt
    13801 attggttaga taagaattag aatagatctc taattctttt atttcttttt ccaatatcga
    13861 atcatggttt tgattagcga tttcttttaa actcagcaat aaatttttat agtcatgtct
    13921 aaatctggca aatttttctt gatctttttc cagtgaatca gtatattttt ttagataaac
    13981 caattctttt cttttcaatt gctgttcata ttgttctcgt tgcctaatag ttattctaat
    14041 aaataaaaat agaacaacaa acatttgaat gattaaaaat attaagacac caaacacaaa
    14101 atgatcaaat acttgatatt gatgagcaac ataagaaaca aataaaccaa ttgtaagtaa
    14161 ataaatcatg aatatagcag taagtttaga actggactgt tcaacaatcc tatttatagc
    14221 taattttcta tataaccaaa taaacatcag caaaaacaaa gaatcaagca ttatctggaa
    14281 tactacataa ccataccctt ttatcccttc aaatgaaaac atatacgtca taaccatgga
    14341 agtaataata gatctgaaat catcaataag catacataat agtaatttat ttaaaagcac
    14401 tatattagtt ttcttctttt cctttaatat aaaataactt ccgattatag gtatagctgc
    14461 ggaaacatca gtaaataatt caacaaaaaa agtgagtggt atcaataaac atacaaaaag
    14521 atattttctt ttgttaagat cagtattagt catataaaat aagagaaaaa agaaaaagtt
    14581 tcccacgaac attcttaata gataagcttt tgtatcgata gtaatcaaga gatcccctcc
    14641 tgattttagg atatcataaa aaaaatttta gttacacttt ttaaataatg aaaaaacaca
    14701 ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc ctttaatttt
    14761 tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt ccattacgat
    14821 tccccctaca aactataaat acttttatgt cactgattca atatacctat ttttctaatc
    14881 aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat ttatctccat
    14941 aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc aaatttctta
    15001 ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta aaatatagat
    15061 aattcaccaa tctatttatt aaaggaccgg gatctaattc atatttttgt ttttctaatt
    15121 ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct ttacttatag
    15181 atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt ttagcacttc
    15241 cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca agtagttgcc
    15301 ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc ataatatttt
    15361 ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa aatttttaaa
    15421 tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct aacattcatt
    15481 aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa taagaattta
    15541 atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa tgcacttcgt
    15601 acataataaa ctttaatcac attagtcttt catacctacg atcgatctct ttgactcgca
    15661 cccataaatt cataatagtt tgtcggcgtc tttggacgtt tcgaagatga gctacgcatt
    15721 ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat ttggatattt
    15781 gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt ccagctttcg
    15841 actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc ttttccattg
    15901 ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt gtctaatact
    15961 ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg gatctgctct
    16021 tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt ggcaaacatc
    16081 caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc ttgacctagt
    16141 gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa gttgtcatat
    16201 ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat tcctatgatt
    16261 ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta ggctggcact
    16321 gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg atctttatat
    16381 tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc atctgactcg
    16441 ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt tacatcatag
    16501 cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt atcccattca
    16561 acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc tataccaatc
    16621 gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt tttacttttc
    16681 tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg tgtaaaacgg
    16741 tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt cccgacttgt
    16801 gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat catagaggca
    16861 ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat gatcgtactt
    16921 ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc gaatgaatca
    16981 gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa acgaacggtt
    17041 ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat accgaaaaat
    17101 gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc aaggatcggt
    17161 ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg taccccaatg
    17221 atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa atcattccat
    17281 agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag tcctggacgt
    17341 ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg agctggaatc
    17401 actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg taaaaatgaa
    17461 aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca gtttgccact
    17521 ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt ctgctaatgc
    17581 ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta caactgcaag
    17641 tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt ctttcccaat
    17701 caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc tagcttctaa
    17761 cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag ccatcagcat
    17821 gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag attcgatatt
    17881 cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac gatcgacttc
    17941 ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt cacctgttcc
    18001 gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc gtaaagcttc
    18061 attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca ggtccagtaa
    18121 ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt cgtccatcag
    18181 caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt gcatcccacc
    18241 agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt ctaatgcttt
    18301 ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc cgtattctgt
    18361 attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca tattgatttt
    18421 atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt ctccatcaat
    18481 atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca atgtggattt
    18541 cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt caaaattcac
    18601 gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg gctggtgatc
    18661 attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta aatgttttac
    18721 ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct ttctgaaaaa
    18781 agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag aaatttgctt
    18841 gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc caagttaata
    18901 tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa aaaagtttgt
    18961 catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg ccgtaaattt
    19021 agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga taactgtggc
    19081 gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca ttgctttttc
    19141 tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct tcgtttgatt
    19201 gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct tctccacact
    19261 tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt gcaataggaa
    19321 atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta cttccatgat
    19381 tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag cattgtacgt
    19441 actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt tttcaacgat
    19501 acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt ttttctttcc
    19561 agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc tctagcaaaa
    19621 taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga tctgttcctt
    19681 ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta aacgagtata
    19741 gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg ccatcctatt
    19801 cctctccttc taatgacaat atagtataaa actggttctt ggattttggc tgacggaaca
    19861 aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac gctacctgtc
    19921 caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa gctaaaccgc
    19981 attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga tggatagccc
    20041 caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa taagctgctg
    20101 cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg gatacttccg
    20161 catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc aatcctgtaa
    20221 tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca actgccagga
    20281 ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca taaaaattat
    20341 aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct gcttttttaa
    20401 caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga ggaaaatgat
    20461 atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt gtttttttca
    20521 agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa ggagctaccc
    20581 aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac tttgcagaac
    20641 aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt tcaatcgtgc
    20701 gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga atgaaggatt
    20761 ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc ctcgccatat
    20821 atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc ggtagaaatc
    20881 ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc atccatcccg
    20941 ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta ccaaacaatg
    21001 tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga taatagatca
    21061 gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca atctgatcga
    21121 ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt gtacatagtt
    21181 gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg tccaatagtt
    21241 tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct atctcgtaat
    21301 cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt ttcttgccgc
    21361 tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt ggatcatatg
    21421 tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt tcgattagtt
    21481 tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca agaccatagg
    21541 aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc atattcagac
    21601 caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga tttcgttcta
    21661 aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc aaataatctc
    21721 ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt aggtcctgaa
    21781 tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt acgcccggcc
    21841 aattccctac atattgattc gaaccagtca agagattgaa caagctagtt ttcccgctgt
    21901 ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca agattagttc
    21961 ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga ttggatctcc
    22021 cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca tatccattaa
    22081 ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct ctcctgcttt
    22141 taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga caaagatttg
    22201 acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa tggcattgtt
    22261 gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat tttgcaaatg
    22321 gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat tctgctttgc
    22381 ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta ttaaaatcat
    22441 tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca ttctcacata
    22501 gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc gaatctttag
    22561 tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa ttttttctct
    22621 attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca aaaacacaaa
    22681 acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg acttttttac
    22741 taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg cactcaccat
    22801 gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca aacggttttt
    22861 aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg aagcagtaga
    22921 ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg cagaaacagt
    22981 tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact aaaagaatca
    23041 aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg caaacttcca
    23101 aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc atcaaatacg
    23161 aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag ttatttcaag
    23221 ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa ggataaagaa
    23281 gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt tctatctatt
    23341 atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt cattcaattg
    23401 tattcaccat catttattga aaagctatat caatttttgg acgagcaaca tttttctttc
    23461 aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac caacgacggt
    23521 tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta ctttgcgaac
    23581 ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg atatcagcca
    23641 gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt gtcttgtttc
    23701 ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc tgctttgcaa
    23761 ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga agccggcgct
    23821 cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa attattcgaa
    23881 gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt ggtttatttg
    23941 aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa tgcagatgaa
    24001 aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt ttatgaattg
    24061 acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa agaatatggt
    24121 gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc aaatccaaac
    24181 attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa attacaacaa
    24241 gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa tccaatcgat
    24301 ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac gccttctgtg
    24361 attaacggca aacaggaata tgaagtatta ggaacggata tctcatgtaa cttaggctcg
    24421 acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg tacgatgact
    24481 cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc gatccaaaac
    24541 ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac ttatcttgca
    24601 aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat ttacttcatg
    24661 ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag aaaacaagtt
    24721 ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa atacgtaact
    24781 ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat ttttatccca
    24841 actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact gtatcatcaa
    24901 aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac gagtgcaagt
    24961 attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg taaaatttat
    25021 tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc ttatgatatg
    25081 gacatgcgta aagtaatcga tgtttatgca atggcacaac aacacgtcga tcaaggcatg
    25141 agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg gaagacaaca
    25201 acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca caaaggaatc
    25261 aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg aagcaatcaa
    25321 tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt ttaattaaag
    25381 gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa ttgaagatat
    25441 cattgataaa tccacttggg agaagctgac agagcaattt tggctagata cacgtatccc
    25501 actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag atacagtagg
    25561 acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg gcatggatca
    25621 gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata ttcaattcat
    25681 ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata cgaaaaaaga
    25741 aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa aagctgagcg
    25801 tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca gcgtcttttt
    25861 agaaacattc ttgttctatt caggttttta tactcctctt tattatttag gaaacaacaa
    25921 attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg tacatggaac
    25981 ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag aacaagaaac
    26041 gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag aacgttatac
    26101 agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc tacgctataa
    26161 tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata ctgccaatga
    26221 tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg acttcttctc
    26281 acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg acgattattt
    26341 gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat aaaaagagtt
    26401 tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc acttgtttct
    26461 tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct caataaatag
    26521 attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat gcaaatcagc
    26581 atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat cgtcaaagga
    26641 tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc taattgttgg
    26701 atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa caaaccgaca
    26761 atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt cgtgattccg
    26821 caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc caaaaagctc
    26881 attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga taatgtttcg
    26941 tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa gaagtgagac
    27001 aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa tggaacggta
    27061 aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact tgcagataaa
    27121 ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc taattgttct
    27181 tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca tttgcttgtt
    27241 tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat taactgaggc
    27301 actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt caataaaaaa
    27361 ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc gaacatataa
    27421 tagataaaac cagcaacaag caatggtaaa aaaactgccg aaagcatttt caatattgga
    27481 ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag tactagctcc
    27541 actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct cctccaaggt
    27601 cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt ttcatttgct
    27661 taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta aacaaaaaca
    27721 cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa acggtgttca
    27781 aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac tatttttatt
    27841 tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc atttattgcg
    27901 caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc tcgatcacat
    27961 cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac actcggtagt
    28021 catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc tcttcaaaat
    28081 aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct aactttttag
    28141 tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc ccgataaaac
    28201 tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca gcaattgccc
    28261 ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg atcgttcccg
    28321 cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca tctgaacgaa
    28381 tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta gcgatctcaa
    28441 cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta cgcatacctg
    28501 ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc atagcgatcc
    28561 ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa acaccctcca
    28621 gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta cctcttacga
    28681 aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg gtgttcaatc
    28741 agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt ttcctttgtc
    28801 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac tattgaaaat
    28861 tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg attttgcaat
    28921 acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat tgcatcagct
    28981 ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga accgattccc
    29041 attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt gtttgcattg
    29101 attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc accaggttca
    29161 ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat tggatgtgca
    29221 tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt tccatctggt
    29281 acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa atgacggtag
    29341 ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga agtcatttca
    29401 ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt gcttcctgtt
    29461 gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc aaatgctttt
    29521 tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc tcctttatat
    29581 tttggtatag cccaattata gcattctttt tttcttttta caataattag tatagaccaa
    29641 attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt tcataagatg
    29701 gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg tggacgcttt
    29761 atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat taaacaacat
    29821 tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat taaatgctgg
    29881 cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga ttaaaatcat
    29941 cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata ttcacaaagg
    30001 ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat ttctaaaaac
    30061 tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc aaacatatag
    30121 cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg aattagacca
    30181 aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt tggggatcga
    30241 tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt caaaggtgat
    30301 tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac caaccaatca
    30361 cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg acggtgcgta
    30421 tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata tcgcagacat
    30481 cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga aacaaaagga
    30541 acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa tcgaaaagac
    30601 cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa aaagccgaca
    30661 aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta aagctgtctt
    30721 tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg atttagtcat
    30781 cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg gtgaaaatat
    30841 tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac tgatcggtga
    30901 gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca attacttttc
    30961 acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa gcagtcaatt
    31021 tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt tagttaatca
    31081 attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg tgaaacttgc
    31141 acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga accatatcga
    31201 tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa cagtaatcat
    31261 cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg agattgaacc
    31321 aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt tttgtcacaa
    31381 ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc tcaaaaaacg
    31441 gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca gaacagcttt
    31501 tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt aaaaacaact
    31561 gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac acctaaccag
    31621 ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc aataaagtac
    31681 ataaacataa atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt
    31741 gtttgattgg cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc
    31801 aaaatggcca aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca
    31861 agtcccatat acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt
    31921 gcagcaactg ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa
    31981 ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca
    32041 aacatgaaaa atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc
    32101 ggcaaacttt taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc
    32161 ggcattttcc agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag
    32221 atcatccgcc aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc
    32281 cccacgagta aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct
    32341 agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa
    32401 gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca
    32461 gataacgctg ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact
    32521 aaaaacagta accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca
    32581 cttgtgctaa cagataaact agctgctact tttgttccaa tcggctggat gtaatacata
    32641 ttggcaacaa caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt
    32701 tttttttcat tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga
    32761 aaagattgga aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt
    32821 ccttgttttt tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca
    32881 cttgcaacca acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac
    32941 gcatacaaca cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga
    33001 ggcaatttca tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata
    33061 tagcctaaca ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga
    33121 ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg
    33181 ttaccaagag cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa
    33241 ccagcaatga ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc
    33301 aatacgatca agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct
    33361 gccttactcg ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac
    33421 cgatagccca ctaccagtat cagaatgatt cccaacgaga tcaatatcaa tcggattatt
    33481 ttctttttca tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa
    33541 aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat
    33601 ttaacagtta cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct
    33661 ttagatacgc cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc
    33721 aagcctaaac cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct
    33781 gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca
    33841 gctacagcta caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg
    33901 tcgttaagtt ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat
    33961 gccattataa tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta
    34021 agctgcatct ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac
    34081 tggcgcttgt agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc
    34141 caatgctgtg atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc
    34201 taatgctttt gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc
    34261 gttactgaat gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc
    34321 ttttttagct gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc
    34381 acgaagttgt ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac
    34441 tactactgat gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt
    34501 tgcgataatt gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt
    34561 ttgcactgaa aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac
    34621 ctattcactt cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt
    34681 tgctcaacca gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa
    34741 ctctatgcca ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag
    34801 gaaattaagg cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta
    34861 gcttagcatg ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc
    34921 taatatttaa ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga
    34981 tcgttacatt tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat
    35041 catggattgc tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg
    35101 gtacatggat atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt
    35161 ttactgcttt tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt
    35221 ttggtcctaa tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa
    35281 ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga
    35341 aatctgcacc agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag
    35401 tttgtgtttt accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt
    35461 ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt
    35521 ttgtttcttt tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt
    35581 tttttaatgc atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt
    35641 ggttataacg gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa
    35701 gctgctgtct tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct
    35761 tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca
    35821 gcgatttctc ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct
    35881 gaaccttttt cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta
    35941 acgaatgtga atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct
    36001 tgatcagctg tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga
    36061 cctagtgcag gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta
    36121 acgatttttt ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa
    36181 ttggagatgc atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa
    36241 tgtgcaaaac aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt
    36301 ttctgctatt tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat
    36361 ttttcgttct catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa
    36421 gcaaacaata acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg
    36481 tggagaaaat gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga
    36541 ttggtccaag tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta
    36601 tatttggcga acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct
    36661 atcgggggca cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg
    36721 atgaacgttt agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg
    36781 ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa
    36841 cacgaaagct ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat
    36901 tgaatggatt caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag
    36961 atgttccagg tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg
    37021 gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag
    37081 atgatcctca agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg
    37141 taaattattt cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc
    37201 aagcaaaagc ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg
    37261 gccgttcccg tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat
    37321 caatcaacga gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa
    37381 aaaaattaga tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag
    37441 ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta
    37501 caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc
    37561 ttgatttgac acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag
    37621 tcatcgcaaa caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt
    37681 ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag
    37741 cagcacaagc agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg
    37801 caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta
    37861 tctctgctga tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg
    37921 ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg
    37981 gcttagctgt tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt
    38041 gataaagctc cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag
    38101 agaggactaa ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact
    38161 tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt
    38221 tcaaaaacag atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg
    38281 ccaatcgcaa ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg
    38341 acaccagagt cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat
    38401 acttcaaagg acgcaggacc tccatctagt tgacgtccac catgattgga tacccagata
    38461 ccatctgccc ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg
    38521 ataactggaa gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt
    38581 tttgcagctg cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt
    38641 tcgctatacg cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct
    38701 tcttcacggt aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct
    38761 tctactgctt tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg
    38821 aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa
    38881 gtgctgatgg aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct
    38941 ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca
    39001 attccgaaaa cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg
    39061 attttctttg tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca
    39121 ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag
    39181 gcaatgttta caatatcgat tggatgttct tcattacttg tatgataagt catagtagat
    39241 ccttctttca tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac
    39301 atttactctc agatactttt atcgctattc tttccaaata aatcgactta caagctgatc
    39361 tacttcttgg ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc
    39421 ctctatttca tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg
    39481 tactgttcca tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt
    39541 ggttatagga atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc
    39601 aggccccttt gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa
    39661 aggcgcgcca attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt
    39721 tcctaaatag cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc
    39781 tatgccgtag ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca
    39841 ctcgttattt ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga
    39901 caaatctcct gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac
    39961 tgcttgctga tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct
    40021 gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc
    40081 cgttttttct ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa
    40141 cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat
    40201 tggttctgtc atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat
    40261 catatgatcg acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa
    40321 gttctgtact ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc
    40381 cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga
    40441 cttcgccaac ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga
    40501 tatgattgac ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa
    40561 cgaatcctgt cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca
    40621 tttcaacaag tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt
    40681 tgacttcagt ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca
    40741 tactttgcgc acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat
    40801 gcaagacata ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa
    40861 taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg
    40921 ttgtggctag cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt
    40981 acgtaaaaca tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg
    41041 agttgttttc tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat
    41101 ttcatgagac aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat
    41161 tacaatattt acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg
    41221 attttgtgta gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca
    41281 tttacttctg cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt
    41341 tttacttgtc aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt
    41401 aatttgaaat taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt
    41461 gtatataaga aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta
    41521 aaaattttag aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt
    41581 ttccgtggaa acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg
    41641 acgcttcctg tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct
    41701 ttcttcaaag tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa
    41761 aatcattttt atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg
    41821 agcgtagaac aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg
    41881 gctgctattg caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat
    41941 atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg
    42001 aaagtatgtg aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt
    42061 gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc
    42121 tttctttttt cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat
    42181 aatacatcaa tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat
    42241 aggttgtcca aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc
    42301 tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt
    42361 gcaataaagc tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca
    42421 tgataccaaa accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca
    42481 atttacggtt aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc
    42541 ctgaggatac aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat
    42601 ttacaacgat tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt
    42661 tctcctagtt tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata
    42721 attataacag acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc
    42781 ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg
    42841 tcataagaat tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa
    42901 acttaaataa ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta
    42961 gcaagcagct gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta
    43021 atggatcatg cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt
    43081 cctagacttc tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca
    43141 agtttctttc attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg
    43201 agtattaaaa tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat
    43261 atattgatca atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta
    43321 ccggtattaa aaaataagca tattgactat acacttcatt atagtgaata caaaggacac
    43381 gatgctgaga ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa
    43441 cagaaaacaa tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat
    43501 caggtacttg atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca
    43561 ggttctggag atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt
    43621 cagctgattt tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa
    43681 ataagtgaaa aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc
    43741 gtccatgcga caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca
    43801 ttttcgtatt tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg
    43861 gtagatgcag gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag
    43921 catccttttt ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc
    43981 attgattttg tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta
    44041 cttatacaga aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta
    44101 cgtatcgttt caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga
    44161 ccttttgata tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt
    44221 tgtcagaagc tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt
    44281 gtcacagtcc tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag
    44341 tttctttctt tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag
    44401 acacaaccat tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg
    44461 atcaagttat tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc
    44521 atttctggca agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata
    44581 aaaatatctt tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc
    44641 gaaaagctaa tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt
    44701 atatcccacg aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac
    44761 agcagcgagt aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg
    44821 ggcgcactct ggtttcgttt actgatctct ttgcgattaa atgctagatt gatgacgctg
    44881 aaaccgctaa aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc
    44941 aacagctttt tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag
    45001 agtgccattt gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga
    45061 atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct
    45121 gggcgactct gtatcttgat cgtcggcatc tttcctactt cgacttgttc ggtaaagtct
    45181 gcaggcagat gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt
    45241 tgtgaggttc catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca
    45301 tcgactacag gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta
    45361 ttttcaacat caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct
    45421 atcagtaaat tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata
    45481 acgcacccct ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa
    45541 ccatagaaga ttggtataaa aactagccaa atcagtgtag aaataaagtt
//
LOCUS       DASJPC010000010        44938 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJPC010000010 DASJPC010000000
VERSION     DASJPC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.010"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1188)
                     /locus_tag="U4A13_000554"
     CDS             complement(37..1188)
                     /locus_tag="U4A13_000554"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN1778879.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="U4A13_000555"
     CDS             complement(1201..1638)
                     /locus_tag="U4A13_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1778880.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="U4A13_000556"
     CDS             complement(1653..3419)
                     /locus_tag="U4A13_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN1778881.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="U4A13_000557"
     CDS             3683..4360
                     /locus_tag="U4A13_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778882.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="U4A13_000558"
     CDS             4363..5490
                     /locus_tag="U4A13_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778883.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="U4A13_000559"
     CDS             complement(5690..6130)
                     /locus_tag="U4A13_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1778884.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="U4A13_000560"
     CDS             complement(6141..8174)
                     /locus_tag="U4A13_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1778885.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="U4A13_000561"
     CDS             complement(8176..9879)
                     /locus_tag="U4A13_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1778886.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="U4A13_000562"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="U4A13_000562"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN1778887.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="U4A13_000563"
     CDS             complement(10962..11987)
                     /locus_tag="U4A13_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1778888.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="U4A13_000564"
     CDS             complement(12196..13065)
                     /locus_tag="U4A13_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEN1778889.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="U4A13_000565"
     CDS             complement(13524..15416)
                     /locus_tag="U4A13_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778890.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="U4A13_000566"
     CDS             complement(15413..17140)
                     /locus_tag="U4A13_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778891.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="U4A13_000567"
     CDS             complement(17156..17806)
                     /locus_tag="U4A13_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1778892.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="U4A13_000568"
     CDS             18010..18306
                     /locus_tag="U4A13_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1778893.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="U4A13_000569"
     CDS             18478..19098
                     /locus_tag="U4A13_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN1778894.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="U4A13_000570"
     CDS             complement(19190..20356)
                     /locus_tag="U4A13_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1778895.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="U4A13_000571"
     CDS             complement(20368..20721)
                     /locus_tag="U4A13_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1778896.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="U4A13_000572"
     CDS             20849..21871
                     /locus_tag="U4A13_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN1778897.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="U4A13_000573"
     CDS             21890..22051
                     /locus_tag="U4A13_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778898.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="U4A13_000574"
     CDS             complement(22310..22744)
                     /locus_tag="U4A13_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778899.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="U4A13_000575"
     CDS             complement(22854..24713)
                     /locus_tag="U4A13_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1778900.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="U4A13_000576"
     CDS             complement(24831..25991)
                     /locus_tag="U4A13_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN1778901.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="U4A13_000577"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="U4A13_000577"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN1778902.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="U4A13_000578"
     CDS             complement(27738..28436)
                     /locus_tag="U4A13_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1778903.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="U4A13_000579"
     CDS             complement(28393..30054)
                     /locus_tag="U4A13_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN1778904.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="U4A13_000580"
     CDS             30272..31672
                     /locus_tag="U4A13_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN1778905.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="U4A13_000581"
     CDS             complement(31924..33003)
                     /locus_tag="U4A13_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1778906.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="U4A13_000582"
     CDS             complement(33089..34048)
                     /locus_tag="U4A13_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1778907.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="U4A13_000583"
     CDS             complement(34060..35571)
                     /locus_tag="U4A13_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1778908.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="U4A13_000584"
     CDS             complement(35593..36156)
                     /locus_tag="U4A13_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN1778909.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="U4A13_000585"
     CDS             complement(36162..37025)
                     /locus_tag="U4A13_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1778910.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="U4A13_000586"
     CDS             complement(37039..37935)
                     /locus_tag="U4A13_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1778911.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="U4A13_000587"
     CDS             complement(38110..39399)
                     /locus_tag="U4A13_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1778912.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="U4A13_000588"
     CDS             complement(39371..40888)
                     /locus_tag="U4A13_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN1778913.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="U4A13_000589"
     CDS             41086..41976
                     /locus_tag="U4A13_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN1778914.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="U4A13_000590"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="U4A13_000590"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN1778915.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="U4A13_000591"
     CDS             complement(42885..44357)
                     /locus_tag="U4A13_000591"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN1778916.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT        12988 a         9387 c         7783 g        14780 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgttttt
//
LOCUS       DASJPC010000011        44670 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJPC010000011 DASJPC010000000
VERSION     DASJPC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.011"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(109..453)
                     /locus_tag="U4A13_000592"
     CDS             complement(109..453)
                     /locus_tag="U4A13_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN1778917.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(446..880)
                     /locus_tag="U4A13_000593"
     CDS             complement(446..880)
                     /locus_tag="U4A13_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN1778918.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1018..1755
                     /locus_tag="U4A13_000594"
     CDS             1018..1755
                     /locus_tag="U4A13_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778919.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1748..2959
                     /locus_tag="U4A13_000595"
     CDS             1748..2959
                     /locus_tag="U4A13_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1778920.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3163..3945
                     /locus_tag="U4A13_000596"
     CDS             3163..3945
                     /locus_tag="U4A13_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN1778921.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4015..4677
                     /gene="trmB"
                     /locus_tag="U4A13_000597"
     CDS             4015..4677
                     /gene="trmB"
                     /locus_tag="U4A13_000597"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN1778922.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4775..6529)
                     /gene="ade"
                     /locus_tag="U4A13_000598"
     CDS             complement(4775..6529)
                     /gene="ade"
                     /locus_tag="U4A13_000598"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN1778923.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6661..7590
                     /gene="tadA"
                     /locus_tag="U4A13_000599"
     CDS             6661..7590
                     /gene="tadA"
                     /locus_tag="U4A13_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN1778924.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7547..8596
                     /locus_tag="U4A13_000600"
     CDS             7547..8596
                     /locus_tag="U4A13_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN1778925.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8593..8871
                     /locus_tag="U4A13_000601"
     CDS             8593..8871
                     /locus_tag="U4A13_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN1778926.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8846..9316
                     /locus_tag="U4A13_000602"
     CDS             8846..9316
                     /locus_tag="U4A13_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN1778927.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9333..9635
                     /locus_tag="U4A13_000603"
     CDS             9333..9635
                     /locus_tag="U4A13_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778928.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9652..10023
                     /locus_tag="U4A13_000604"
     CDS             9652..10023
                     /locus_tag="U4A13_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN1778929.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            9995..10354
                     /locus_tag="U4A13_000605"
     CDS             9995..10354
                     /locus_tag="U4A13_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778930.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10526..11533
                     /locus_tag="U4A13_000606"
     CDS             10526..11533
                     /locus_tag="U4A13_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1778931.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11558..12742
                     /locus_tag="U4A13_000607"
     CDS             11558..12742
                     /locus_tag="U4A13_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN1778932.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13179..14762)
                     /locus_tag="U4A13_000608"
     CDS             complement(13179..14762)
                     /locus_tag="U4A13_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1778933.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15340..15483)
                     /locus_tag="U4A13_000609"
     CDS             complement(15340..15483)
                     /locus_tag="U4A13_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN1778934.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15705..16184)
                     /locus_tag="U4A13_000610"
     CDS             complement(15705..16184)
                     /locus_tag="U4A13_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1778935.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16257..16541)
                     /locus_tag="U4A13_000611"
     CDS             complement(16257..16541)
                     /locus_tag="U4A13_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778936.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16619..16813)
                     /gene="ssrS"
                     /locus_tag="U4A13_000612"
     ncRNA           complement(16619..16813)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4A13_000612"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16885..18171)
                     /locus_tag="U4A13_000613"
     CDS             complement(16885..18171)
                     /locus_tag="U4A13_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN1778937.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18309..18980
                     /locus_tag="U4A13_000614"
     CDS             18309..18980
                     /locus_tag="U4A13_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN1778938.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18946..19434
                     /locus_tag="U4A13_000615"
     CDS             18946..19434
                     /locus_tag="U4A13_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN1778939.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19447..20394
                     /gene="prmA"
                     /locus_tag="U4A13_000616"
     CDS             19447..20394
                     /gene="prmA"
                     /locus_tag="U4A13_000616"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN1778940.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20395..21159
                     /locus_tag="U4A13_000617"
     CDS             20395..21159
                     /locus_tag="U4A13_000617"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN1778941.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21392..23605
                     /locus_tag="U4A13_000618"
     CDS             21392..23605
                     /locus_tag="U4A13_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN1778942.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23616..24062
                     /locus_tag="U4A13_000619"
     CDS             23616..24062
                     /locus_tag="U4A13_000619"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN1778943.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24233..25492
                     /locus_tag="U4A13_000620"
     CDS             24233..25492
                     /locus_tag="U4A13_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN1778944.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25581..26528)
                     /locus_tag="U4A13_000621"
     CDS             complement(25581..26528)
                     /locus_tag="U4A13_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1778945.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26922..28214
                     /locus_tag="U4A13_000622"
     CDS             26922..28214
                     /locus_tag="U4A13_000622"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN1778946.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28229..29998
                     /gene="aspS"
                     /locus_tag="U4A13_000623"
     CDS             28229..29998
                     /gene="aspS"
                     /locus_tag="U4A13_000623"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN1778947.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30107..30982
                     /locus_tag="U4A13_000624"
     CDS             30107..30982
                     /locus_tag="U4A13_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1778948.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31248..33131
                     /locus_tag="U4A13_000625"
     CDS             31248..33131
                     /locus_tag="U4A13_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1778949.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33206..33490)
                     /locus_tag="U4A13_000626"
     CDS             complement(33206..33490)
                     /locus_tag="U4A13_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1778950.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33480..34214)
                     /locus_tag="U4A13_000627"
     CDS             complement(33480..34214)
                     /locus_tag="U4A13_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN1778951.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34343..34966
                     /locus_tag="U4A13_000628"
     CDS             34343..34966
                     /locus_tag="U4A13_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN1778952.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35158..36279
                     /locus_tag="U4A13_000629"
     CDS             35158..36279
                     /locus_tag="U4A13_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN1778953.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36286..39414
                     /locus_tag="U4A13_000630"
     CDS             36286..39414
                     /locus_tag="U4A13_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN1778954.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39433..42627
                     /locus_tag="U4A13_000631"
     CDS             39433..42627
                     /locus_tag="U4A13_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN1778955.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42773..43480)
                     /locus_tag="U4A13_000632"
     CDS             complement(42773..43480)
                     /locus_tag="U4A13_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1778956.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            44245..44643
                     /gene="spxA"
                     /locus_tag="U4A13_000633"
     CDS             44245..44643
                     /gene="spxA"
                     /locus_tag="U4A13_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1778957.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        14464 a         7888 c         9387 g        12931 t
ORIGIN      
        1 acgttgtcgt ggtagtttca aacaaaattc aaaatttcat cacattccta ggaaatacgc
       61 aaacttttct cacttatata gttaaatctg ttgagaaaat aatcattatt atatttgttc
      121 aggatactct ggcagatcag cttttagttt ttctacttgt tcattgattt gtgtgatacg
      181 tggttcggct tggaatttaa aatcttcgat atcttttttg attgcttctt ggaaagcagg
      241 aatcacagat tcagctgttt cttttgtttc gatcaatgcg cttttaaaat gattcaggct
      301 gttatttaaa tcatttgtca gagttgttgc ttcttccaat tcttcgatca gtttctttct
      361 tgtttcattc ccgctcctgg gcgccaataa cagacctccg acagtgccag cagcagctcc
      421 aaataacagt cctttgaaaa agtttttaat catcgcagga cacctgcttt acgattgttt
      481 cttgaatagc ttttagttct tccgatccat aactatcttg atgattgaca aagtgaatag
      541 aaaaatcatc ctctttgctg tagcgaggga ctaaatggat atgtgaatga aaaacagatt
      601 gataggctag ttcacggttg ttattcaaga tattaagacc tttcatttct ggaaaagctt
      661 tttccaatgc ttgagcaatc ttaggcacac gcgaaaaaac ctctcctgca agttcgggat
      721 catattcaaa aatatctgct acatgacgtt taggcacaac taatgtgtgt cctttcgttg
      781 cttgagagat gtctaaaaaa gcatagacct tgtcgtcttc atatatttta tagcttggga
      841 tttcttgatt gatgattttg caaaaaatac agttttccat ataaaacatc ctttctataa
      901 agaataaata cttcagctta actttaccat aagttaaagc tattgtaagg aaaaaaacaa
      961 aataccgtca ttttcttcgt ggtataataa ataaaaataa atggcggagg aaaaagaatg
     1021 agtttaatta tcgatcatgt cacagggggg tacggacatc tccctgtgtt aaaagatatc
     1081 agtttcgatg tacagcctgg agagatagta gggttgattg gactgaacgg agcagggaaa
     1141 agtacgacca tcaagcacat tatcggtctg ctggaagctt caaaaggaac gatagaaatc
     1201 aatcatctat ccttaaagaa aaatacggaa gaatatcgca aacaaattgg gtatattcca
     1261 gagactcctt ctttatatga agaattgaca ttgagagaac atatcgaagt cactgcgatg
     1321 gcctacgata tacctatgga tatagcaatg aaaagagcag aaaagttgtt ggagactttt
     1381 cggttatcta atcgtcttga ctggttccca gctaattttt caaagggaat gaaacagaaa
     1441 gtgatgatct tatgcgcctt tttgatcgag ccttctttat atatcatcga cgaaccattt
     1501 ttaggcttgg atccactagc tatcaatgca ttgcttgaat tgatggtaga aatgaaaaaa
     1561 gaaggagccg gtattttgat gtctacgcat attttagcta ctgcagaaaa atattgtgat
     1621 cgttttgtgg tcttacataa cggagagata cgagcagacg gttctcttga gatgttgaga
     1681 aaagaatttc agctgccgga ttcttctttg gacgaaatct atattgcttt aacaaaggag
     1741 caggcacatg agtagttttt ttcaaaaacg tttagcaaga catcaaaaga aaatgatgaa
     1801 atatatgcgt tatgtattga acgatcattt tgttttagtt tgtcttttct tgattggcgg
     1861 tgtgggatta tattattcta attggttgaa aaccctgcag ccaccgtttg caattggagg
     1921 gctcataatc tctgtatttt ggatgctctg tttattcgtg ggtaagatgg ctacttttgc
     1981 agaggcagca gatattgttt ttttacttcc taaagaaaaa gaaatgcaaa cctatttgga
     2041 acgagctttt cgctattctt gcatttttcc gttgatcttt ttgatattgg catgcggatt
     2101 ggccatgcca ttagtggtgg ttagtacgga gcaatccttt tcgatgtttt tcgtctatct
     2161 gatcatatta tggagtttga aattcagtca tctgcaaatc aagagagcag aattgtttcg
     2221 actagatcaa caagttattc gttattggaa attaggctgg tttttttctt ctctgatctt
     2281 tttgtttcta tcattttatt ggctaatatg ggcaggaata gcaggtgcat tagtacaggc
     2341 aagtctatat tactggtttt tgtggaagca acaagaaaaa ggattggatt gggagaaaat
     2401 ggtgcatgta gaagaacata ggttgcatca gatctatcaa tttatcaatt tattcacaga
     2461 tgttcctgaa attacggcaa agatcaaaag aagacgctat ttggatccta tattgactca
     2521 tattaaaaga gaatcaaaaa acacttatct ttacttgttt gcaagaagat tggcaagagg
     2581 ttctgatttt agtggtcttt atttccgatt agtaattata ggtgggttat tgattgccgg
     2641 agtagtcgac ttttatttta gtctgattat cggtttctta tttttatatt tgatcggttt
     2701 ccaactgtta cctttagcga accagttccg ctatatggtt ctcgttcagc tttatccaat
     2761 ccataagaat cagaagaagc aggcgattca gtatctactc acatgggtat taattatcgc
     2821 gtcggttatt tttggtttga ttgctgcagt cgtattagat gggcagcagc gatgggttcc
     2881 gctagcggct tatttgattg ttactatgct ctttgttaaa ctatatgtac caactcgttt
     2941 gaagaagatg tcagactgaa aacaaagctg tgtagggtaa aaatgagaat gaaaaataga
     3001 gcgttgacag taagaatgct gttcgttcta ttttttttga gaaaagctgg ttttttgaat
     3061 ggcaattaaa tgaaaaagaa cgtatatgtg cttgacgtgt agtgttcagg cgactagaat
     3121 aataagtact gcaaaacaaa gagtaagagg acgggagaat gcatgatgga gtttcagtta
     3181 gaccaagagt ggcggttacg tcccatcaaa ggcgacacag gaaaaacata cataggcatg
     3241 cgtgatgagg acaaagtctt tattaaacga aacacgactc cgatgttggc tgctctttca
     3301 agggaaggaa ttacaccaaa acttgtttgg acaaaacgaa cggggaatgg cgatactttg
     3361 acagcacaag agtggctgga aggtcgcttg ctgactccta atgagattgg tcaaagaaac
     3421 gacgtgattg atgttttgta ccatttacat cattcaaatt ctcttaaaac catgctgacc
     3481 cgtatcggtg gtcgtgtcat gacgccggag aagatgctgc aaaaatatga aaaagaatta
     3541 ccggaagaac tccagaaaaa tcaatttctg caaattgtcc atcgttttct tcgtgagcag
     3601 atcccgtcaa tagataagga aaacttatca gtagtacacg gagatgtaaa ccatcgtaat
     3661 tggttagtat gtaaaaatta tttatatcta gtcgattggg attcaatcat gtttgcagac
     3721 cctgcggtgg atattgcaac gattttagga aattatgtgc cactgtctag ctggaatcag
     3781 tggctgatca gttatggcat acgcccgact gacgaaatgc ttgaaaaatt tcattggtat
     3841 gcggccatga atttgctcca agaaatcacc agatattgca aacgtgaaga tcatcgacgg
     3901 atgaacgaag aaattttaca attaaaacga attttcagtg gctgaggttt ggcccaaact
     3961 ctgcggagat ttggccagac ctttttaatt agaggaaagg aatggagata aataatgcgt
     4021 gtccgaaatc gtccgggagc agcggaaatg ttagcggctc acccgaattt tgtaattagt
     4081 gatccaacat tgtggaaggg aaaatggaat gaattgtttg aaaacgatca tccaatccac
     4141 attgagattg gtatggggaa aggccagttc atcactggaa tggcaaaagc ccatcctgaa
     4201 atcaattata ttggagtaga aatgcaggtc agtgttgttt cgattgcact agacaagttg
     4261 atcgaacaac cgcttcctaa cctgaaactt ctacatgtgg atggatcagc gttgacagag
     4321 tatttcgctg acagcgaagt agatcaaatc tatctgaatt tttccgatcc atggccaaaa
     4381 aaacgtcatg aaaaacggcg attgacatat aagacttttc ttgctgtaga tgaacagatt
     4441 cttcgtccaa atggagaaat ccatttcaaa acagataatc aaggattgtt tgagtattct
     4501 ctggcaagtt tttctcaata tggcatgatc ctcaaacagg tatggttaga tttacatcaa
     4561 agtcaatttg aaggcaatat tatgacggag tatgaagaaa aattttcttc aaaaggtcaa
     4621 cgtatttatc gagtagaagc tcaatttcaa gataagacca aaaagaaaag tctgtgaaac
     4681 taactttctg ctttcataaa aacattaata tccataaata aatctcagaa aatgaacatt
     4741 acagatgtaa tgtattttct gggatttatt tgatttaaaa tgtatttatt tcatcggttt
     4801 ttccacaagg tggaatcaat tggaagcgag aaaatgaaaa taatcctttg tcggtgatct
     4861 tcaattcggg tatgacagat aatgttaaga aagaaagtgt caaaaaagga tcaaaggttt
     4921 tagctgctcc tagttgatgt gctttagtta ttaattcatt caactgttca tagacgatcg
     4981 aaaaaggttc ctgtgtcatg attccgccta taggaagaga aacacaagct atttcctgct
     5041 gtttgttgac aacggtcatc ccgccacctt tttgaatcaa aagattagca gcatacagca
     5101 tatcctcgtc gtttgtccca acgacaacta gattatgcga gtcatgggca atcgttgtcg
     5161 ctatcgctcc ttgtttcagc tgaaatcctt tcactatgcc taatccaatg tttcccgttt
     5221 gatggtgacg ctcgatcacc gcaatcttta attggtcttt ttcgattgaa ggaatgaatc
     5281 gttcgccttg tcggtccact ttttctacca aatctttagt cactagacta ttcgggataa
     5341 tttcgatcac atgggcttgt gaagaagaaa gaagcagctc taacgaatgt ggatttaatg
     5401 gttgagcgtt tagttttggc agacaacacg tatatgaatt tgaatgatca gcaaataaat
     5461 ggtcttggag ttgcccagat tgaacaacta atgttccctt atagaaaact tcatgaattg
     5521 gcagcttggt aagatcgtct gttaagaaga aatcagcttg atacccaggg gcaatcgcac
     5581 ctagtgtttt caaatcaaaa cattctgctg cattcaatgt tgccatctga atggcttgta
     5641 atggatcgat tccaagtgca atagcttgac ggacgatatg atcgattgat ccttctttta
     5701 ctaaatcatt gatcagttta tcatcggtga caaagaagca tcttcttgaa ttttcaggtg
     5761 tgatcgccgg tagtagcgat gccagatctt ttgcaacggt cccttctcga accattaaat
     5821 acataccaag atccaaacgt tctttagctt cttttgcagt tgtagcttca tgatccgtcc
     5881 gaatacctgc tgcaggataa acatttaact cttccatgcc gataccagct gcatgcccgt
     5941 caattttttt cttatgctgg tgcatcaatt ggatcttttc taggatgtct gtttcattat
     6001 ttgctactgc ttgatagttc atgacttcag ctaagcctaa aacttcgggt ttttgtaaaa
     6061 aaggggctaa tttttttgca tttaaatttg caccattggt ctcaaaaggg gttacaggta
     6121 cacaagaagg tagcataaaa aagatattca tgagtgtttg tcttgcatct tctatcataa
     6181 actcaagacc tgtactccca gcaatgtttc ctatttcatg gggatctgtc actacagtag
     6241 taactccatg aagcaaggat acttttgaaa attcgcttgg tgataaaagt gagctttcga
     6301 tatgcacatg gccatcgatg aatcctggca ctagatattg cccattagca ttatgtacgc
     6361 tttttccttc gtattcaccg attcctacga tcattccttc gcagattgct acatcttttt
     6421 ccatgatttc tttcgtaaag acgttgacga tcttagcatg cttgatcact aggtcagcag
     6481 gctttttttt acttgctact tcgatatatt tccttaatgt tgtttccaat ggagtgcctc
     6541 ccaagataaa tttttataag tataacataa attcggcttt tgccaatatt tttttgtaaa
     6601 ttcattttat ttgaaacaaa aagatcaaaa agacgtatgt tttttgtgga ggggagaata
     6661 atggaaataa aacgattatc ggatcaaatg ataagtgaaa gttttgccaa acatgcccaa
     6721 gacgtatata tattgccagc ttcagaagta ttttacatct attttcgcta tggaaaaaaa
     6781 cgaacattct ggaagaaagt ctcttcggca gatggacagc aactgatctc aagatttaag
     6841 tatttgggac agatggatat cggagaaaaa agaaaagctc agttaggtgc atttacttat
     6901 tctttagaaa aacaggaagt tcgtctgcgg ttgtcttctg taggagatta ccttcaacga
     6961 gagagtttag tgattcggct attatacggt acttttgaaa caccccatcg ttgctttagt
     7021 tctcgagatt ttgcaactat tttacagaaa accgtcagac gtggattata ccttttcagc
     7081 gggacagtcg gatcaggcaa gacttctcta atgtatcggt tggctttgga agaaaaattg
     7141 caagtgatta ctatcgaaga tcctgtagag atagaagaac cgttttttct gcagctacag
     7201 atcaatccga aaatagcaca gacttatgat acacttttga aactcgcatt acggcatcgt
     7261 ccagacttgc taatcattgg cgagataaga gatgaatcaa ctgcacatgc tgcggtaaga
     7321 gcagcactaa cagggcaccg ggtttttgca accgtacatg cacgcaatct tgaaggtaca
     7381 ttggtcagaa tccatgaact gacaaagaaa aaagaagagc tatctgaatg tttagctggt
     7441 gtcatttatc aagaactttt ggaagatgcc aatcaccatc cttctgtttt atggagttat
     7501 caatttatgg aggaagaatc agtaaaaaaa tcatgggagg aaagttatga agaagctcag
     7561 gaaaatattt ggcagtcaga actattctaa aggattaaca agaagccagc aaaaccagtt
     7621 tgcacaaacc atgggagaac tgcttttatc tggttttagt ctgcaagaat ccctttattt
     7681 attactcacg atccgttcgt ttccacctgt tattttggca aatatccaac atcagctaaa
     7741 ggaagggcaa gcgtttagtg aggtactttc gcaaatagga tatggagcag agcaactggt
     7801 acagatcgag ttagctgaac gtcatgggaa tctgccgaca actttgttgg aaattgccaa
     7861 acagtttcgg cttgttgaag gatttcgcaa tgatttgaaa aagctgcttg catatccgat
     7921 gctattgctc acctttttgc taggtatctt gtttagtatg cggttgttcg ttttgcctca
     7981 gcttattggt tcaggaatgg tcatgcaaga gcattggggt attcagctga tacaagtctt
     8041 tcattggtat gttctactat tcatcctagc ggttactcta ttggctttac tagtaaggta
     8101 tcgtctgatg aagttgtctc ctgttgatca gagtgaatgg ataagcagac aattattgat
     8161 tggaccgata tattccttgt atcaatcggc ttatttgtca ctagagattg gaaagctttt
     8221 tcatgaaggt ttcgaattaa ggcaaatcat agtctgctta aaggaaacga aacaagaaag
     8281 tctgattcag catcttgcct ctaagttgat tcaaggattg gaggcaggta tgtcattggc
     8341 tgagcagttc cagaattatc gttttctatc agaagaattc agtaaaattg ttttacaggg
     8401 agaggcaaag ggaagtttag gtaaagaact gattttttac agcgaatgga caagaaaaca
     8461 gcttttcaaa cgtcttcatc actggatgca ttttattcag ccaatcattt ttttgggcgt
     8521 agcggctttg attttattag tttatgccgc tgttttatta cctgtttacg gaagcataga
     8581 ggaggttttg ccatgaaaaa gagaaaaata tcttattata gcggttttac attgatcgaa
     8641 atgctaatcg tcttactgat catcagtgta ttagtgttgt tgttcgtccc taatttgtcg
     8701 cgttatagga atcatgtaga ccaagaaagt cgtgaagcga tcatccagtt ggtagacaca
     8761 caaaaagagc tctatgcgct tcaaaataat ggaagagttc ctactgtgga ggaattgttg
     8821 aatgaaggct atatcaaacg agaacatgca gaaatctatc agcggccttg aaggtaaggt
     8881 tttagcatca ggatatacgt tgtttgaatc actcttactg attttattac ttacaacgat
     8941 cctttctttt ccagttctcg cattttctgc atggaagaat gaattggcag tctatcaatt
     9001 tttcggccaa tttgaaaaac gaatctattc tacgcaaaaa atcgctattg tgaatcagat
     9061 ccaaacaggc ttttattgga atagtgaaga gaatcaaatc atttttcatg ttccgaatcc
     9121 agataaaata aattgggaag tattagatat tcctgatgag attaccttga aacgccatgc
     9181 ttctattact tttgctgcta gaaccgggaa tgaaagttca ctaaaagctt atcaatttta
     9241 ttgggaagaa aaagagcgga cgattacgta tcaattccag atagggagtg gtcgctatac
     9301 aaaaagaatt gaataaaaca ggatttgttt tgttggagtc ccttacagcg cttatgatcg
     9361 cttctgtctg tgtctactta gtgagctttg gccagctgtt tttgcttcag gcagaaaaga
     9421 aaagtgggga ggaactgcta tttattcgaa tgatttatga agatatcaag acgcagcgga
     9481 tgtatcacaa aagaccagag cgaagggaag aaatgatccc atacgatatc caaattgtcg
     9541 ataataatgg agtaatcgaa aaagtgtttg tcaatatgga gaaagaagcg atcagtattg
     9601 aaaaaacaag taattccagc atttacgctg attgaatgtc taattggact gatgatattg
     9661 agcagtctat tgctcacatt caatcttttg attcagcaaa ccatccaagt aagtgaattt
     9721 ttgcagcgta aagatcaaaa agagtggcta atatttttag cacaattaga agaagaactc
     9781 aagagcagtc acaatgtctc tgtgaaaaat cagcaactgc actacacgat cgaggataaa
     9841 cagtatatca tcgaaagata tcaggcaatg atacgtaaaa gaagaacttc cggcggacat
     9901 caacctatgt taacaagtat cactgagctt caattattgg aaaaagaaca cacaatttct
     9961 ttttatgtac actttgaaaa tggagaagat ggatatgcca tatggacaaa aaacgatcag
    10021 tagaaaatgt tataagggct ctattttatt ttctgtcttg cttttatttc ttttatgtac
    10081 gtttttattg atggcagcat tagaaagcta ccgtttgtca atggatctta atatacgaat
    10141 caaaaattat tatatagcaa aaataatgaa gcagatggct ttatcggaac agtctacgct
    10201 tgccgaaagt tcggaaggag ttttttacta cacaactgga tctgtaacat atcagtgggt
    10261 acaacagtcc ctattcttag aggtggaagt ctcaccgttt atctttcggt tcacagaaga
    10321 agtaaaaaat gatacagata ctagtacaga atgatggaat gttcgtgaaa acgccctctt
    10381 ttctgttata acactgtttt taataaaaaa atgtttgccg tttcacaaat aaaaaacagt
    10441 gaaaacgatt gaaattttgt caattgttcg atagaattaa acaggtgaaa aaattgttat
    10501 aatatatcta gaaatgaggt gcaccatgtt accagaaaaa atggaacaag ggtttaatca
    10561 aaacctggaa gctattcagc ttttacagaa tgcattggga acttcctttt tagaagccta
    10621 tgtcgaaaat gcagaaaatt taatagatga ttatcaagta cgtgtggtgg atggagtgcc
    10681 gacaaaagag acaacgcaac ggatcacagc tctttatgaa gagttgaaga aactttcttt
    10741 cgagccagaa gaatggcgca gactgtctca attactttta ttgaagggca gccagacaga
    10801 acatttgcag gcgaaccatc aactcactcc tgatagtatc ggttttttat ttgtttttct
    10861 tattgaacaa ctatacacaa ataaaaaagc accggttaag attctggata ttgcggcagg
    10921 aatgggcaac ttgttattaa cagttctttt aaacctatcg aatgcggggt accaaacaga
    10981 aggaattggt gtggatattg atgatacatt gttggctgtc gcagcttcta caagtgattt
    11041 gacacaagca aatgttcaat actttcatca agacgggttg caggaattat taatagatcc
    11101 tgtcgatttt gcaatcagcg atttgccaat cggttattat ccgaatgacg aaaaagcaaa
    11161 agagttttta acaagtaccg aagaaggcca tagttatgca catcatttat tgctagagca
    11221 atcaatgaaa tatgtgaagc cggatggttt tggtttgttt ttgatgccga gtggattttt
    11281 agaaacggat caaagtgaag aaatcaaaaa atggtttaaa gaagaaggtt atcttcaagg
    11341 aatgattcaa ttgccagatg aattgttccg taataaacaa tcgcaaaaaa gtattttgat
    11401 cttacagaaa aaaggaccac aagcacaaca agtaaaagaa gtcttgcttg tgaaacttgc
    11461 ttcattaaaa gaaccagaga aggtgacgga atttttcaac gagtttaaaa actggaaatc
    11521 atctgctctg taatcattca acgagaagga gagtcatatg tctaaaacaa ttgcaatcaa
    11581 tgctgggagt tcaagtttga aatggcaatt atatcaaatg ccagaagaaa ccgtagttgc
    11641 gaaagggatc gtcgaacgta tcggtttaca agattctatt tttaccatca agtacggcga
    11701 taatcaaaaa ttcgaacaaa tcatggatat cgaaaaccac gaagtagcag taaaaatgct
    11761 gttagatcag ttgattgaac tgcaaatttt aagctcttat gatgaaatca ccggagtagg
    11821 gcaccgtgtt gttcacggag gagaaactta tggggattca gtagtgatcg atgagaaagt
    11881 aatggaccgc attgctgaat tagcagaatt tgctcctttg cataatcctg caaacttaat
    11941 ggggatcaaa gcgtttaaaa agattttacc tgacattttg agtgtggctg tattcgatac
    12001 atctttccac tcaacaatgc caaagcaaaa ttatttatat agcttaccgg tgtcttatta
    12061 tgaagatttc aaagtaagaa aatatggctt ccatggcaca agtcatcgtt atgtgtcgga
    12121 acgtgcggca aaattattga acaaaccgat cgaagaacta aaaatcatca cttgccactt
    12181 aggaaatggt gtatcgatca cagcagtcga tggaggtaaa tccatggata cttctatggg
    12241 attcacacca ttagctgggg tgacaatggg tacacgttca ggtgatatcg atccagctgt
    12301 tctcccatat ttaatggaaa aattagagat tgatattgat gaaatgatca atgttttgaa
    12361 caagaaatct ggtctgctag gcctaacagg attgtctagt gatatgcgtg atcttgaaaa
    12421 gaactatgaa aaagaacata ttcgtttagc ttatgacatt ttcgtggacc gcatccgaaa
    12481 atacatcggc ggttatgtga caacgatgaa tggtgttgat gcaattgtct ttactgctgg
    12541 tatcggcgag aatgacggac atgtacgtag tgaaatcatc aaaggtatgt cttggtttgg
    12601 ttgtgaaatc gatccagcgt tgaatgaatt gcgtggtgaa gaagtagata tctctacaaa
    12661 agaatcaaaa gtaagagtat tagtgatccc aacagatgag gaactaatga ttgcccgtga
    12721 tgtggagcgt ctgcgcggct aagccaaagc attcgttagg ataaaagatg aaacgcatgc
    12781 aagagggaat tcttgcatgt gttttttttg cggagaagac cgcaaaatgg taatttaact
    12841 tcaatttccc ttctttttta caggatcttc ttagggaatt ttgcttggaa aagtggtcaa
    12901 aagggaaaag actggcattt tatttattga ttttcaggaa aatcccaata ttttattttc
    12961 attcatcttt ataagggaca ttacatatgt aaaaagaaaa aacattttta gatcagcctc
    13021 gtgggctgtc gactttattc ttcgctttat gtttagctag aaaaccgatc cgaaacattc
    13081 attgatcgaa taataaaaac ttgattttcc acctgtactt tgcctgaatc gtgaaagcac
    13141 aggtatgaaa atcaagtttt ttgttgcgaa aaaacgtcct agtcaatcag ttggccgagc
    13201 ggctggcggc tgatctgctc ctgatcaata acgagtgctt cttggaagtg ttgctgcaaa
    13261 aagtagcggt catggctgac tgctaggagc gttccttgat agtcttgcag catttcttca
    13321 atttcttctc gagcatagct gtcgaggtga ttggttggtt catccaaaat caggacgttg
    13381 attttttcta aaaagagttt cagcaacgcc aaacggattt tttcaccacc ggataaatct
    13441 ttgatccgtt tacccacatc gctttgataa aagccacttt tggctaattg ccgtcgagcg
    13501 acttcttcgt ttccagtcaa tgtgatggcg taagccagca aacggctatc gggatcggca
    13561 aactgcagct gctgcggcaa ataaccgatt tgcaagctac tacctaattt gatggtgcca
    13621 ccgtaaggct tttcctcacc gagtaataat ttgagtaagg ttgattttcc tgcaccattg
    13681 tcgccgatga tggctaaatg atctccacga tagactgcaa aactgctgtc tttgaatagt
    13741 acttgatcag cgtactgctg gtgcaagttt tgaatgatga aaacttcttt gccagatttt
    13801 ccaccattag acaagctttg cagccgattt ttcgggggag gtggcggttt gacgagtgtc
    13861 agtttcgcca ggcggcgttc cagttctttg gcttttttga agaaggactc attgtctcct
    13921 tcatgtgccc actgacgaaa acgacgaatc atgactttca accgctggac ttctttttgc
    13981 tgcagttcgt aattttttgt cagctcagcc aagcgagctt ctttcaagac tcgaaaacgg
    14041 ctgtagtttc cgggatactc aatcaatgca ccttcttcca gttcccatgt ttttgtggtg
    14101 acttgatcga gaaattgccg atcatgagaa acgaccagaa ccgatttttt tgtattctgt
    14161 aaatagtttt ccaaccagcg aatactgtta agatctaaat gattggtcgg ttcatcgagt
    14221 aaaagtaaat cggcatcggc tgctaaaatt ttggctaaag cgactttaac tcgttcgcct
    14281 ccgctaagtt ctgttaaccg tgcttcctga cagtgtggaa tccctaagcc ttgcaagatc
    14341 gctgcaatcc ggtcttctaa tacgtaaccg cctagttctt caaagcgatg ctgcagctca
    14401 ccgtaacggg ccagacactt ctctaagttt gtatctggtt ctgtcatcaa tgcttcgtag
    14461 cgactcattt gctgtttgag gtcctgtatc tcagcggcag aatggagcaa gtagtggttg
    14521 acggtcgctt cggaaactgt aagttcttgt tcgaccgtcc cgatctttaa gccttttttg
    14581 cgactgataa caccgctgtc gacaccttct ctgccggtca agatttgtaa aaaggttgat
    14641 ttgccagttc cgttgatatc gactaaggca attttttcac cagcttcaat ttgacaggat
    14701 aaatcctcaa ataatggatt cccgccgatt gtttttttaa tagaaattgc ttgtatgatc
    14761 atgatctctc tccttgagac cactcaaaaa ggccatggtt atccctggcc tgtgttattt
    14821 aaactcagat tatcatgaga aaaatacctt gagatggcca ttgattttac gtgaattcaa
    14881 ctgttttgga caaacttgtc ccgtcaacag caactgtaaa accaattgca tggctaaatg
    14941 cgatatggta tcgttggtaa ttttcattac ttgttatttt agtcatgcgc atcatagtat
    15001 tttccctcct tcgtgtagtt gtcttttcca gtgtacctga aagtgtcaga aagttcaact
    15061 agcgctgaca tttacttaat atttatcgat gtgtaattct gtcagagagc tttttttatc
    15121 tgactgaggg taatgtctcg tggaccacat cattgtattc tgagctaaac cagcgttcag
    15181 gcgaaatcat atggtgtttg ataaaaaact ttgtctacat gacttccttt tgccatttgc
    15241 ttaataccgt tgcattaaac agctcttctc tttggaaatc tcaccaacat cgcgttcagg
    15301 agtatcgttg tggttgcaat ggcaagcggt agcctttcat caattcttga atgatttacc
    15361 gaatgtagca cttgccttct cgcggtaccc gaacccgaaa taaaatttta gtttggcgat
    15421 aaaatcggta tccgccatcg atacttcata ttttttcgta tcagattcat cggaaacttc
    15481 cacattgacg aaccaataag ctttggcatg cttaggtcgt tttaaaccac accagggcga
    15541 ctaatcgccc tggtgttcac tttttttatt ttacttgaac tcattgactg ttttcccttt
    15601 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    15661 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    15721 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    15781 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    15841 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    15901 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    15961 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    16021 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    16081 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    16141 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    16201 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    16261 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    16321 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    16381 attacgccta aaacagcata tgcagcaccg taaaattgta ggaatccttg atttttatca
    16441 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    16501 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    16561 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    16621 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    16681 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    16741 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    16801 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    16861 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    16921 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    16981 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    17041 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    17101 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    17161 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    17221 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    17281 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    17341 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    17401 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    17461 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    17521 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    17581 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    17641 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    17701 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    17761 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    17821 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    17881 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    17941 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    18001 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    18061 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    18121 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    18181 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    18241 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    18301 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    18361 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    18421 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    18481 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    18541 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    18601 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    18661 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    18721 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    18781 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    18841 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    18901 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    18961 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    19021 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    19081 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    19141 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    19201 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    19261 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    19321 gacgatgacg aagctgtatt cgagcttcac tgggataatc aacaactagc agaattagta
    19381 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    19441 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    19501 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    19561 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    19621 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    19681 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    19741 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    19801 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    19861 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    19921 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    19981 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    20041 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    20101 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    20161 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    20221 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    20281 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    20341 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    20401 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    20461 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    20521 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    20581 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    20641 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    20701 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    20761 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    20821 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    20881 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    20941 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    21001 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    21061 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    21121 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    21181 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    21241 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    21301 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    21361 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    21421 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    21481 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    21541 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    21601 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    21661 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    21721 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    21781 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    21841 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    21901 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    21961 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    22021 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    22081 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    22141 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    22201 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    22261 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    22321 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    22381 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    22441 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    22501 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    22561 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    22621 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    22681 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    22741 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    22801 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    22861 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    22921 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    22981 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    23041 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    23101 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    23161 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    23221 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    23281 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    23341 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    23401 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    23461 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    23521 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    23581 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    23641 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    23701 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    23761 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    23821 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    23881 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    23941 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    24001 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    24061 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    24121 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    24181 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    24241 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    24301 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    24361 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    24421 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    24481 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    24541 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    24601 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    24661 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    24721 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    24781 aggtgtcgag aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    24841 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    24901 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    24961 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    25021 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    25081 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    25141 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    25201 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    25261 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    25321 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    25381 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    25441 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    25501 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    25561 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    25621 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    25681 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    25741 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    25801 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    25861 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    25921 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    25981 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    26041 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    26101 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    26161 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    26221 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    26281 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    26341 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    26401 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    26461 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    26521 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    26581 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    26641 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    26701 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    26761 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    26821 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    26881 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    26941 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    27001 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    27061 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    27121 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    27181 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    27241 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    27301 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    27361 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    27421 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    27481 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    27541 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    27601 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    27661 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    27721 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    27781 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    27841 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    27901 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    27961 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    28021 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    28081 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    28141 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    28201 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    28261 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    28321 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    28381 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    28441 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    28501 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    28561 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    28621 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    28681 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    28741 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    28801 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    28861 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    28921 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    28981 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    29041 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    29101 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    29161 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    29221 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    29281 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    29341 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    29401 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    29461 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    29521 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    29581 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    29641 tgcagaagct tatgatatcg tcttgaacgg ttatgaatta ggcggaggtt ctatccgtat
    29701 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    29761 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    29821 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    29881 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    29941 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    30001 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    30061 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    30121 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    30181 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    30241 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    30301 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    30361 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    30421 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    30481 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    30541 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    30601 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    30661 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    30721 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    30781 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    30841 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    30901 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    30961 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    31021 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    31081 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    31141 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    31201 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    31261 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    31321 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    31381 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    31441 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    31501 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    31561 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    31621 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    31681 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    31741 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    31801 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    31861 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    31921 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    31981 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    32041 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    32101 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    32161 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    32221 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    32281 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    32341 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    32401 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    32461 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    32521 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    32581 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    32641 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    32701 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    32761 taaaggaaca agatttgttg aaagacactg ttctgattgg actaggaacg aatggctcat
    32821 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    32881 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    32941 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    33001 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    33061 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    33121 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    33181 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    33241 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    33301 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    33361 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    33421 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    33481 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    33541 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    33601 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    33661 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    33721 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    33781 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    33841 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    33901 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    33961 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    34021 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    34081 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    34141 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    34201 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    34261 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    34321 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    34381 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    34441 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    34501 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    34561 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    34621 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    34681 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    34741 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    34801 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    34861 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    34921 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    34981 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    35041 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    35101 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    35161 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    35221 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    35281 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    35341 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    35401 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    35461 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    35521 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    35581 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    35641 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    35701 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    35761 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    35821 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    35881 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    35941 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    36001 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    36061 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    36121 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    36181 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    36241 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    36301 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    36361 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    36421 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    36481 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    36541 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    36601 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    36661 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    36721 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    36781 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    36841 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    36901 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    36961 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    37021 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    37081 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    37141 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    37201 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    37261 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    37321 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    37381 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    37441 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    37501 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    37561 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    37621 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    37681 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    37741 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    37801 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    37861 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    37921 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    37981 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    38041 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    38101 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    38161 agacgaaaaa gcaagatggc caatgaaaga atagagctgg aagaaataag acaacaagca
    38221 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    38281 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    38341 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    38401 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    38461 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    38521 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    38581 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    38641 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    38701 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    38761 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    38821 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    38881 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    38941 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    39001 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    39061 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    39121 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    39181 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    39241 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    39301 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    39361 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    39421 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    39481 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    39541 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    39601 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    39661 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    39721 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    39781 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    39841 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    39901 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    39961 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    40021 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    40081 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    40141 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    40201 ataaatgaac aaaacgatca aggaatacaa ttttctgatc aaaacaaacc tttatcattc
    40261 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    40321 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    40381 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    40441 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    40501 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    40561 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggt
    40621 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    40681 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    40741 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    40801 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    40861 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    40921 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    40981 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    41041 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    41101 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    41161 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    41221 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    41281 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    41341 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    41401 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    41461 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    41521 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    41581 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    41641 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    41701 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    41761 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    41821 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    41881 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    41941 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    42001 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    42061 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    42121 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    42181 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    42241 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    42301 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    42361 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    42421 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    42481 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    42541 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    42601 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    42661 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    42721 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    42781 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    42841 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    42901 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    42961 tatgaaattc tttctcagcc agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    43021 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    43081 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    43141 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    43201 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    43261 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    43321 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    43381 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    43441 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    43501 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    43561 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    43621 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    43681 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    43741 ttattttttt aatacagtct caatataact attcaaacat aaaataacgt tatttgattt
    43801 atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg cttatataac
    43861 agagaaaata agcgttataa taaagaacag tattatttaa aatgacagaa aacattacta
    43921 ttaaagaaat aaaaaacata aaaaatctta agaaatcggt ttcatttagc ttttgagata
    43981 aaataatagt acagttttat acgctgtttc atatgtttag aaaatttgaa ttagatttga
    44041 aaactctaat ttttaaatga taaaaaaagc gagaatgctg gttttactgt atattaacct
    44101 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
    44161 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
    44221 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
    44281 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
    44341 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
    44401 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
    44461 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
    44521 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
    44581 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
    44641 tagaaggata atacgaaact tggaaaagac
//
LOCUS       DASJPC010000012        44552 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJPC010000012 DASJPC010000000
VERSION     DASJPC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.012"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(192..662)
                     /locus_tag="U4A13_000634"
     CDS             complement(192..662)
                     /locus_tag="U4A13_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN1778958.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="U4A13_000635"
     CDS             992..2632
                     /locus_tag="U4A13_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN1778959.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="U4A13_000636"
     CDS             2786..4108
                     /locus_tag="U4A13_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN1778960.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="U4A13_000637"
     CDS             complement(4176..4610)
                     /locus_tag="U4A13_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1778961.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5512)
                     /locus_tag="U4A13_000638"
                     /pseudo
     CDS             complement(4675..5512)
                     /locus_tag="U4A13_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(6254..6880)
                     /locus_tag="U4A13_000639"
     CDS             complement(6254..6880)
                     /locus_tag="U4A13_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778962.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7175..7510)
                     /locus_tag="U4A13_000640"
     CDS             complement(7175..7510)
                     /locus_tag="U4A13_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1778963.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7497..7781)
                     /locus_tag="U4A13_000641"
     CDS             complement(7497..7781)
                     /locus_tag="U4A13_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1778964.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7837..9360)
                     /locus_tag="U4A13_000642"
     CDS             complement(7837..9360)
                     /locus_tag="U4A13_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1778965.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9353..10528)
                     /locus_tag="U4A13_000643"
     CDS             complement(9353..10528)
                     /locus_tag="U4A13_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1778966.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10532..10717)
                     /locus_tag="U4A13_000644"
     CDS             complement(10532..10717)
                     /locus_tag="U4A13_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778967.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10683..12377)
                     /locus_tag="U4A13_000645"
     CDS             complement(10683..12377)
                     /locus_tag="U4A13_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1778968.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12374..12847)
                     /locus_tag="U4A13_000646"
     CDS             complement(12374..12847)
                     /locus_tag="U4A13_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN1778969.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12916..13071)
                     /locus_tag="U4A13_000647"
     CDS             complement(12916..13071)
                     /locus_tag="U4A13_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEN1778970.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13205..13585)
                     /locus_tag="U4A13_000648"
     CDS             complement(13205..13585)
                     /locus_tag="U4A13_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1778971.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13589..13804)
                     /locus_tag="U4A13_000649"
     CDS             complement(13589..13804)
                     /locus_tag="U4A13_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778972.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13807..14214)
                     /locus_tag="U4A13_000650"
     CDS             complement(13807..14214)
                     /locus_tag="U4A13_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEN1778973.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14480..15955)
                     /locus_tag="U4A13_000651"
     CDS             complement(14480..15955)
                     /locus_tag="U4A13_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEN1778974.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15945..16793)
                     /locus_tag="U4A13_000652"
     CDS             complement(15945..16793)
                     /locus_tag="U4A13_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEN1778975.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16830..17192)
                     /locus_tag="U4A13_000653"
     CDS             complement(16830..17192)
                     /locus_tag="U4A13_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778976.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17193..17351)
                     /locus_tag="U4A13_000654"
     CDS             complement(17193..17351)
                     /locus_tag="U4A13_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778977.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(17492..18116)
                     /locus_tag="U4A13_000655"
                     /pseudo
     CDS             complement(17492..18116)
                     /locus_tag="U4A13_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18113..18424)
                     /locus_tag="U4A13_000656"
     CDS             complement(18113..18424)
                     /locus_tag="U4A13_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778978.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18468..18761)
                     /locus_tag="U4A13_000657"
     CDS             complement(18468..18761)
                     /locus_tag="U4A13_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1778979.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18955..19602
                     /locus_tag="U4A13_000658"
     CDS             18955..19602
                     /locus_tag="U4A13_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1778980.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19663..20808
                     /locus_tag="U4A13_000659"
     CDS             19663..20808
                     /locus_tag="U4A13_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1778981.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(20889..21194)
                     /locus_tag="U4A13_000660"
     CDS             complement(20889..21194)
                     /locus_tag="U4A13_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN1778982.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="U4A13_000661"
     CDS             complement(21219..21650)
                     /gene="ruvX"
                     /locus_tag="U4A13_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN1778983.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(21647..21913)
                     /locus_tag="U4A13_000662"
     CDS             complement(21647..21913)
                     /locus_tag="U4A13_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN1778984.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(22168..22893)
                     /locus_tag="U4A13_000663"
     CDS             complement(22168..22893)
                     /locus_tag="U4A13_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1778985.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(23254..23324)
                     /locus_tag="U4A13_000664"
     tRNA            complement(23254..23324)
                     /locus_tag="U4A13_000664"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23290..23292),aa:Gly,seq:ccc)
     gene            23441..24193
                     /locus_tag="U4A13_000665"
     CDS             23441..24193
                     /locus_tag="U4A13_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1778986.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            24209..24688
                     /locus_tag="U4A13_000666"
     CDS             24209..24688
                     /locus_tag="U4A13_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN1778987.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(24939..26519)
                     /locus_tag="U4A13_000667"
     CDS             complement(24939..26519)
                     /locus_tag="U4A13_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN1778988.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(26512..27255)
                     /locus_tag="U4A13_000668"
     CDS             complement(26512..27255)
                     /locus_tag="U4A13_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1778989.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(27431..28207)
                     /locus_tag="U4A13_000669"
     CDS             complement(27431..28207)
                     /locus_tag="U4A13_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1778990.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(28455..29291)
                     /locus_tag="U4A13_000670"
     CDS             complement(28455..29291)
                     /locus_tag="U4A13_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN1778991.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(29302..30231)
                     /locus_tag="U4A13_000671"
     CDS             complement(29302..30231)
                     /locus_tag="U4A13_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN1778992.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            30423..30776
                     /locus_tag="U4A13_000672"
     CDS             30423..30776
                     /locus_tag="U4A13_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1778993.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(30850..31506)
                     /locus_tag="U4A13_000673"
     CDS             complement(30850..31506)
                     /locus_tag="U4A13_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1778994.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            31732..33666
                     /locus_tag="U4A13_000674"
     CDS             31732..33666
                     /locus_tag="U4A13_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1778995.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(33752..34975)
                     /locus_tag="U4A13_000675"
     CDS             complement(33752..34975)
                     /locus_tag="U4A13_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1778996.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(34969..36867)
                     /locus_tag="U4A13_000676"
     CDS             complement(34969..36867)
                     /locus_tag="U4A13_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN1778997.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(36861..37979)
                     /locus_tag="U4A13_000677"
     CDS             complement(36861..37979)
                     /locus_tag="U4A13_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1778998.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(38062..38895)
                     /locus_tag="U4A13_000678"
     CDS             complement(38062..38895)
                     /locus_tag="U4A13_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1778999.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(38888..39805)
                     /locus_tag="U4A13_000679"
     CDS             complement(38888..39805)
                     /locus_tag="U4A13_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1779000.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(39828..40007)
                     /locus_tag="U4A13_000680"
     CDS             complement(39828..40007)
                     /locus_tag="U4A13_000680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779001.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(40032..41156)
                     /locus_tag="U4A13_000681"
     CDS             complement(40032..41156)
                     /locus_tag="U4A13_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1779002.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            41339..42247
                     /locus_tag="U4A13_000682"
     CDS             41339..42247
                     /locus_tag="U4A13_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779003.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            42388..44001
                     /locus_tag="U4A13_000683"
     CDS             42388..44001
                     /locus_tag="U4A13_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1779004.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        13463 a         8880 c         7429 g        14780 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     5101 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5161 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5221 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5281 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5341 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5401 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5461 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5521 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5581 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5641 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5701 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5761 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttcaaaaaac
     5821 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcag gagaacaccg
     5881 tttaaggaag agactgtgaa gtcaacgttt tgacctgatt attagagaaa caaatggcaa
     5941 aatagttctt catatttcgc cgtttttcga gcgaatgccg aggggcagct gattgaacat
     6001 cgtttatttg gaaaaaggga ttgtgacaag acgtttgtca caatcccttt ttttatatct
     6061 tattaattca ttcttttgtt ctattcttct tcttccgcca tgaatgagaa acccatatca
     6121 aagaactata aaggttatca aaacagcgtt tatgactaaa tgaattagag ttatctatca
     6181 ctatttttca agcgatttaa accaaatggc atacaatatg gcatacaaaa acttcaataa
     6241 gccgtggggg tttttaaatt tcttcttgtt ctttaaaatc aatgagtgga gctacataat
     6301 ttgaatccac tttttcaaac gtcacttttt gcatcgacga atgttttctt tcccaagata
     6361 acgtaaatag atttgactct ggacttataa agtctacttc accttctagt attactttct
     6421 tatgcaaata ttcctctaat aaatatagac ttgctaaagc atacaacaca ttttccaaat
     6481 ttgcttcctt ataatttgaa gaacgaccat gttttactct gttatatatt ttccaccaat
     6541 ctggtgtatc ttcaggccac ccatctccaa aaggttttag tgaagtcaat gttaaagaat
     6601 tcataaccct aacaccatta tccaaacaca ttaattcgct agatttaata acagctttaa
     6661 aatcagttat gcttttattg ctactgctat caaatccgca aagttctttg aaaataatct
     6721 cacactccga accaatcgct agtagtagtt tttgatattc aatagagaat gttttgaaat
     6781 tattctcatc gagcatcaca aaatctgtgg tattaataaa ctcattttct aaatgtagat
     6841 aatacttcca ataaaaattt gtaaactctg ctttgtccat taaatcacct cttttgaatt
     6901 ataacaaaaa aaacacgtcc acaacagacg tgttctttga tttctacaca atcagcttgc
     6961 caaaaccatc caatcagtcc tagcatgcct gtattaaaat tgaacgttca tcatctatca
     7021 tctttcttag tccagcatgt ttgcaaccac acctacaatt ttcagtccaa caatagattt
     7081 cgactagcta agtcttgcaa aattcaggca gctaaccctt catttactac cttaattata
     7141 ctaaataccc acaacgttta caagtgtaat cattctaaga aatctgttta gctatgatcg
     7201 ttgtataatc tttaaacgcc gttccttcat tgattgaaat aattttataa ctcttgtttt
     7261 tccaagctac tttcatatcg tttgttagtt cataatcttg atcgtatcga ataataaacg
     7321 tcaacgttcc ttcaagaatg gtacctacac tagcaagcac gtcttttagc atttgtgaac
     7381 gcacattagc ccaacattca aatacagttt cttcactatc caccatctgc ccatcttcat
     7441 cttttacccg ttttgtcttt ttaaacttga ctctctcagt caattcattt acattcttaa
     7501 tgattggcat aataagcacc tctcatttgt tgtactaatg cttgtaccgt tactggtatc
     7561 cgttcgctgc tggcttcttg acggttcaaa taccaatgtt gagtaagtaa tgacaccgcc
     7621 aaatcaaatt gctgataatt atccatatct ttatccgttg cgctactatc aatagcactt
     7681 tttatatatt cgcctgctgt atcaatcaat tgttgaataa agtcatcatc aatagtgtga
     7741 tcgatccgca tacttttttt tatgctatcc aaaattacca ttttatctct cctaatgaaa
     7801 aaaaggcagt gggataaacc gccgcccaat atttttctat acttttggtg ttacaactgg
     7861 cgtaaactca atatacactg aagcattttg atcaattttc ttataatcgt tgcgaacgat
     7921 cactgcaagt ccttgtgagt agtaatcgaa tttttcccat tgagtcgtta cttgatttct
     7981 acgagcaaca aagatagatt gtgcaatatc ccccataatc atagggaatg ttccagcttt
     8041 aggattggca aacaatgtat ctgcaatcaa tactactggc ataccgaata atgatttacc
     8101 actaggtgct gtcacatcgg gttgtaaaat gtaacgtcca tctgaatctt tcaatgtatc
     8161 taggtggtaa tatccgcttt ggtttagaat taccatttta tttaatgctg ggtctaatgc
     8221 cacattgtat agttgtttca aatcgtccaa cgtagcagcc gttttcttag tgaatgtttt
     8281 taataaatcc atgatatgct tattgtcagt gttttctacc aatttcgcta actgatcttt
     8341 gacctcttgt acaatattca ctgctgaatc ttccacaacc tcatttgata aggcaatctt
     8401 accagcacga gtttctactt tataatcaac tgaagtaaac atttcagcgt caatatcacc
     8461 aatttcagca agttcagctt ttgttgctaa cactgcttgt tggttagttg ctactggata
     8521 tttaccttgt ccatttgata ctgtttttac tgttgcatat tgagctaagt tataatttga
     8581 acgtttcaaa tcaaagactt caccgattac ttcttcggga acaactgccg ctgcatttac
     8641 agtagtgact ccatcacgca cttcgccttg tgaacggata tattcttcat acccacgcaa
     8701 ttctgttttt tcgttatcga ttaaagtttt ttccatgtta ttactctcct tttgtgcttt
     8761 agaagtggat tctaagctat tgtttgtttg ttttgcttgg ttgttactca taaacgattc
     8821 gtaagaacgc ttatttactt ggacatttga tgaatcataa gcgggaacgg tcacgacact
     8881 aatttcatta agtgctttga ttttatctat tgatcgttca attgtgccat cttctttttt
     8941 gtcgaatgaa tctaatccaa gcacgaaccc aaaggacata gaatcaacaa ccccttttga
     9001 gatattctca tacacatcat ttgaataggt cgtgtcggtt agctctgctt caaaatgtaa
     9061 tcctgtatca tcgacattca atttcaacgt acctgctttg acactagcta gtggctttga
     9121 ataatcgtgt ccatataata agaacacatt tgataaatcc acctctttta atgcttcggg
     9181 tgtaatcact tctacaaagc cgcctaaatc ttttgacggt tgcccgaatt tcaacgcata
     9241 gcctgaaata gttttgccgt ctgttggttg tttttcattt tctttctcta ctttgggaga
     9301 atcggctgtt agctcagctt cttctgtcag ccgtttttcc ttttcttctt cattattcaa
     9361 ctggtgtaac tccttcctgt tcttttaatt gatatttctc caacgtatct aaatacgtaa
     9421 agttcaaact tgctagtaaa cgatcgccac catccatggg gggtagcccc attttcgacc
     9481 gcccctcatt aatcgtgaga agtgaccctt tgacctgatc caatacattt tttacttttg
     9541 tttcaggatc agtctctagt aactgatcgg aattaaagcg gatattggtt tcaagtttcg
     9601 tatctaattc actcacaaac acattaaagt aatgaatcag cgtactttgg atatactgga
     9661 gattactttg aacggtgcta gaatgttcat tttcaacgcc taaacgctca atcggtacac
     9721 caaacgcctt ggcaatttgt tttgtgttcc aatcattgga attgataagc ttcaaaacat
     9781 cggtgtttat ttctaatgtt ttgtagtcca ttgtttcatc aagaatgatc gttctaagag
     9841 cattatctcc actacttgaa ccgttggctt cttcaaactt ttcacgaatt gcgttctttg
     9901 ctgatccgtc taaatctgac ttatgaacct tcaaaatacc tgacccactg acaccacgag
     9961 taaagaaatt gtgcaatgtg cggttgccag cttgttgaat tttcagttca tcacgtaacg
    10021 cataaagtgg tggtaatcct gttaaaccat cttgggtgaa atatttgaag tgcaaaatat
    10081 cgctagactt cacacgtctt tttttatcac caatctgata agacaacgta ccattatcta
    10141 attgagtaac cgttacactt gagttaggta ataagtgaat ttcttctacc ttttcaccgt
    10201 tgcgtttaat ctctgcaaag ctattaccgt ttagcaacat attgaccgcc aaagcaaact
    10261 ttaaatgcca accatccatt tctgaattag gtttctcgtt tagtaacttc accagttcat
    10321 tatcagcttg tggcatattg tttttgacca attgaatcgg acttgaagca atatcactag
    10381 caataattcg aacggctgca aacacatcac tatttcttaa tgcacgaacg cttgtaaagc
    10441 tggtggtgta atcatccgat tggattgata ccacatgttg taggaaagga tctcccgttg
    10501 tattttgtct cgctttaaaa aatgccatct tttaacctcc cttctgttct gtatttagca
    10561 tgaccgctac cccaataagc actatgccca gcgctaacaa gccatagtag acatttgtta
    10621 gtaaggtgat agcaaccaac acacacacca atccaagaag taaaagaacg gtatgaatgt
    10681 aattagaaag aaaattcttc acttgtataa aattcattgt ctgctttcgc tccttccgtt
    10741 gtggtgaagt aatccattgc aaacacatag gcattgatta atgcagctat tggatcaatt
    10801 ttgttactgt tctttgattt attaatctga atgccgttgt tatccgtttt taaaatagcg
    10861 ttgttcactg catgggtgag aatcgtattt ttattgtgaa taacgttgcc ttcataaact
    10921 tgttctctaa aagttcgggt cggaacgttt agagtaatcg ttccttgtct tacttctaac
    10981 attgggtagt tggctttttc agctttactg ataagcgaat tagcgttata cgggtcataa
    11041 caaatcccca tacattccaa atcattttct tgaatcaaat cttgaataaa ctgaaatact
    11101 tggtcatagt ccacgattcc actttctaat tgagtaatgg aacactcacc agctttttct
    11161 agttctcgat aaggcaaacc gtcgcgcttt tctttgtctt gaagcccata tttggtggct
    11221 acaaagctat gtgaatcaca ataaagttcg ccgttatcta gcggtacgat ccatgaaacg
    11281 ctagttaaat catctgtttt agataaatcc actccgatat aaacgggctt gcctgtaata
    11341 tcttgtggtt ctacttcggt tgcctgccag tcttcagcaa tcatatagct gtcctcactc
    11401 gcttgtctcc ataaattgaa gtttttcacc aatacagaat taaggttatt ttgtttcaaa
    11461 gcaaggttca catcatcttg aatggttgga atcattactt ttttgatttc ttcactttca
    11521 aaaatcggat tggctttgat ccatttctct tgatcgtgaa tttcttcggg atcgtccaat
    11581 tcccatatcg ctataaagta cctgtcggct tgttcttcgc cttttagcac acgatctaac
    11641 aataagtatt cctcgtacat aggaacgttt aaatcaagcc cagcggtact aataacacac
    11701 aataagccgt tcttttgttg ggtcattcct gacttaatga cgttgtacgt tttacgagtt
    11761 tttgcttcgt gccactcgtc aagaattgcg gttgttggtg caaacccatc tagcgtgctg
    11821 gtttcactag ccagtgccat agcgaacgaa ttagagggca agtgctgaat cttagagttc
    11881 ataattttca gttgtggtct taaaaactta ctagacttga ccacgtttct aagtgaatta
    11941 gacagcatat catagcccaa tttagcttgt ttcaaagcgt tacttacaaa caatacttgc
    12001 cttgcttctg ctggttcttg ttccataatc aacgcattgg cagccatgcc acttgcgata
    12061 tacgttttcc cattcttacg gctcatactg gtaaacgctc gattgaatcg cctgtaattc
    12121 ccttctttag tacgccagcc ataaagactg cctagaatga atttttgaaa tcctaacatc
    12181 ttgattgctt taccgtccgt agatggtagc aactcaatga atttaatcgc tttggtggct
    12241 tgttcttcat cgaacacata agggaagcta tctgacttag aacgctccaa atcgcttata
    12301 tgacgctcac acgcttggat aattttcttg ggtgcaatca gcttacctga taggactttc
    12361 tcaatgtaat cattcatgaa ttcatcaact ctttgaacgg gtcttcgggt tcatcatttt
    12421 taaccttgtt caaagctagt tttgatcgtg attctaaggt catacctagt gaggtggctt
    12481 gttttttcaa atcactcata gcttgtgatt gaacccgaac cgctggattg gctttcttta
    12541 ctccgttttc gcatgtgata aacgtacctg attttcttat ctcggcttcg gctgttttca
    12601 ttcgagcaaa ggctaaacaa taggctacca atgaaccata atctgcttca ctcaatggat
    12661 aatctttttt taatgttggt acgatacgat tccattcact tctagcgctc gctgccatcc
    12721 agtcaggggg cgtgatattt attaatggtt gctgcttgaa caattcttct ctagcgtcca
    12781 cacggattgc tttttcttca cttgatatat tgccttttgt atcgtgtaat attttagggt
    12841 gtcccatgtt gtcctccttt cagacaaaag aaaaaggaac gtaagcaatt aagctcacat
    12901 tccttcgttt ttcgatcaga atttttcttt atctaattta tctatataaa tgatttttcc
    12961 gttctgcatt tgaatggtca cttgaccaaa atgaggtaac tcaatggtgc gctgtttccc
    13021 tgttttatca gtaataacaa ttgtattttt caataaatct cctactttca ttatatactt
    13081 tttcacttgt gattacaagt gtaatcgtta tttgtaatta cccttatgcc ttttcacaac
    13141 ggttatttgt accaaaatga gtaggcatcg attgtcagta agtttcacac tatgccccct
    13201 taactcaaag ggtactcttt tctcgtcttt gtctttcatt ggctgtcttg gaattatggt
    13261 gtgcatggca taaagaacga aaattagttt catctaaccg tctattccaa tcatctttta
    13321 gttcaatttt gtgatctaca acatcagctt tccttatcaa gccttcttct aggcaatcct
    13381 cacatactgg atgatttaac cgatacaagt atgaagcctt tcgccatgtt ctactttgat
    13441 agaattgaaa atatttacct tcgctttgct ttctttcttc acgcttaatt gccgtttgct
    13501 ttgctttatg cttcccacaa tacttctgcc tataatcaat caatgtttta catccagcat
    13561 gattacattg ttttttaact gccatagatt accaaccaaa gcgatctcgt tcgtcagaat
    13621 catcttgttc ccattttgat ttgtattcaa tataaggcga aactatcttc aagcacgttc
    13681 cgtctttaaa attaacttta gtgattttat cttcctcaaa actaataaag tctacatctt
    13741 cttgagtgtt tgaatactca atgtgtttct caccatcgaa gtatctaata ctttccatat
    13801 gcataattat tctacctcca ataaattagt tatcaattca tcatctgtta gtagcaaagc
    13861 caagaactta tcatggatgt ttaaaaacaa attatcctca ttcgatgtaa gctctcgttg
    13921 tctcgctcga tcatataggt taagcagaca attaaattca tccattagct cacctgtatc
    13981 atcatagttg ccactatcta ccaatgatcc cactactagc gattgacacc acgctactga
    14041 ctcctgcagc caatcaggtt cttcttccat tatctttaat acttctggat caaatgtgga
    14101 ataatcttct gttaccaatc ccacactaaa tgccagcgtg ttcattagaa accgatccat
    14161 ttcatctgta tattgaaagt tcggtgcttt gtttgttgaa ataatattga gcatttactt
    14221 tcctccttga ttcgtaacac gtggtaacac ctacgtaaca ggtacccgtt accactcaat
    14281 cccaacgggg acaagggttc tagcgttttg gtaacaggta acgggtaaaa tccagtaaca
    14341 ttattcccca gcatattagc taactatttc aaaagtttta aggaaataag gtgttacccc
    14401 gttaccaaat tgctgtatcc cttggggcag taagggtaaa ctagtaacgg gctaggtgtt
    14461 actgacccgt tactacccgt tactttttgt actttccagc tcgattttta ttttcatagg
    14521 catttgactt attgtaaaat cctcgtcgtt tatttctttt tggcagttgc ttccgttccc
    14581 aaccaggaat actgctaata atcaaggcaa tctttttagt ttctgcatct gatcgaccat
    14641 tcaataaatc ctttgcttgt ttatcaaaaa cagcttccaa aatatcagcg gtgagtacgc
    14701 tatcaactaa ataaaactct tgttgcatag tagctccatt atagaaatgc tgtaaatttt
    14761 tctcattaca tttactgtca ggatagttct gataataaga acgtctcgcc cacgctggag
    14821 cttcttccca tttatacggg atttccattt ccaaatattt agtgattgct tctttaattg
    14881 gattctccat cttggcatcc tcttgatagt cttttgcaat ttctaagact tctttatctt
    14941 catcgggact aaaatagatt cgttgtcctt tatcaaaaag cacctttgct tcagctaaca
    15001 cttgaagaaa atattcatcc tttgttttaa acggattcat ctttggctta ttcttacaag
    15061 gcaaaggata aaaacggcgg tttcctgtat catctttcaa atactgccca tcattcgacg
    15121 ttccaataaa tacacattgc cttgcccaag ctatgacatt ccgaccataa ggaggtctaa
    15181 ttttatcgat cttggcactg atgaagtttt ttatctgttc aattttagtt ttgttcattg
    15241 aagataattc tccaagttca ataattacat tgccttgaag ctgcatataa tcatctttct
    15301 tttcacctag cgaatctaat gaatcggtga aaaaatcatc cgtgtaaagt aatgctggaa
    15361 cggtactctt accaatccct tgcggtccat ccaaaactgg aacgatctca aactttattc
    15421 ctggtttata gatacgtgcg actgatccga ctatccaacg ttttgttact tctcgtgtgt
    15481 aaaggttgtc gtctacccct aaaaggtcaa taaacagtgt ttccagtcta ggctgttgat
    15541 cccatttaac tttttcgata cgttctttta ctgggttgaa aacattcttt ctagccacat
    15601 ttactactgc tttgtgtatt ttgttgtcat caaaaactac attgtagttt tcttcaatat
    15661 atgccgttaa ggcatcctca aaactgtctt cccattctcc agcggtacga ttgatccaag
    15721 gagaatcttt taataggtga atgtatccgc taaattcgtt ataagcaatt gttccagcta
    15781 aattgctatc attggttaaa atcaattctg cattcacaac actgttactt ttaatctgat
    15841 cgtctttatt gcatcttaat cgtgacatcc aggttttcaa ttcaatgata ttgtcttcac
    15901 gctcgctggc ttcgggaaag tctaaaatac tatctaattt atgcttatga gccatgagct
    15961 ttccccctca aatctttttt aatcatcgat cgaataattt gattcacttc tttatctttt
    16021 agcggtggtt cattgtacgc atttgctgta taaatcaatt ctgctaaagt ttgattatct
    16081 acaccgtgcc atagtaacca accgcataac ttagttaaat gatcgtttct actaccttcg
    16141 ccagcgcctt gaacaatcat atctaatttt tttgctgtcc ctgtttttgc tacgggtcgg
    16201 tattgagtct ttatttcttg cggttttttt agcagtaggt tcactagcca atcgggagct
    16261 ggtgcaatat tatttagaga tacgttttca ataggttcat atgctctccc aacacttctt
    16321 gaaataatca tagttggcgc tattggaact cctttagtga taatatcaat cccgcttaaa
    16381 cttttacctt tatcatcacg cccgaagata tcagcaaatg cacctgtttc ttgttggata
    16441 attacaccat caggaatttt aaagaagaaa tggattcctt tacctggtgt gatttccgta
    16501 taagtagaag gaaatggatc atacattgaa gcaagttttt tataattctc aacgccgttt
    16561 cgtgtttcag aatgttgatc taggtcaact accaccattt gatgatcttc aagcattaac
    16621 ccaatatttc tagttgcatc ttccgtccac cattcattaa tttgattaga atcgttagtt
    16681 gcatcattcc aaccactaga gccttttatt ggaattttag ttccaggaga taagggataa
    16741 attgaccatc ccgacttttg taattccgtt gctgcttgat aattatttgc caataaacct
    16801 ctcctccttt ttcaaaatac tggtataaat cattcagaca cctccatgag cttcaactgc
    16861 tcaagttcgt atttcaaccc atcccgaaca aattcagcac cttttaattg attctgatac
    16921 tttacccatg tattaaatgt tttcaccctt gcttcttgca tatcagtaat gtttaaccat
    16981 gcggttaaaa tctgattttt tagctcgttg atttctcgac aaattatttt gtgttctgca
    17041 tgttttttga gtacatacaa aactgttagt ttttcattga atgaatttcc aaagtaggtt
    17101 ttcccgatat tattttctag ttcagcgatc ccttgattaa tttcctctaa ttcaaggcta
    17161 tcgagttggt ttaatagttt tgttttgtac atctacttat gccctccagt tcttaattga
    17221 tagtcgtact cgtcataagc agtgaagaga tataacgcta atcctgcaaa gatagctatt
    17281 tttgcaatta atggtaataa gccgaaaatt ccgccgatta aaatgcttcc tataattaag
    17341 tttcgtttca taatggttcc ctccgatttt ttaatatggt ataatcggaa caaagaatat
    17401 cgggttttat cccaattaag ttgcctttat cctgtccgcc aagactgtta tggataaggc
    17461 tttttctttg ttctcgatca tgttaatgct cctaagttgt ctgcaattca gcagaacgat
    17521 tataaatttt ctcactcact gaaaactgca acccatattt ttctttaaca gtcattaatt
    17581 ctactgtctc atctaaaata ggttcacgat caaccaacat ttcaggagtc atatctttct
    17641 tttttaccat tttggaatga ccataaatag ttgatacagc tttattggct atcacattcg
    17701 ctttaatgta gtctactggt tttggttcag taatagcgtg gcttaattta gtcattgctt
    17761 ctttttggtg ttctttgtct agcatgcgga atacttgaaa gccttcaagt cctgtggact
    17821 ggcgtagttc tttgatgatg tcgaaaatcc agtcttgaaa tgcctcggct tcttttttac
    17881 ggcttttgaa tgccatgcgg taaatatttt ttttcgttga tgatgtttac atcttgtgtt
    17941 ctgcccaatg aatcgatgac cttactagta gtaaccccat ctttaggcaa agagtgaatg
    18001 gctctagtaa cctgtttaag acctaacgct tccgcaacat ctttcgccac tgcccaccat
    18061 tcatcattaa tatcgacaaa tcttataatg tgtccgttcc aaatttcagt tttcattcaa
    18121 cgtcctccag ttcaagccct agcaagctcg ctaacaactc aacttgctga tacaaaattt
    18181 cttgatactc ttcaatagaa agtcgttctt taaactctgc ttgtactaaa tcgttcaagc
    18241 cacgcaatac tccgcttacc gttggttctt gtggtagcaa tttttgatag tccatttgtt
    18301 cgtacaaaca ttcaaacgtt tcttctagcg ctttgccttc caagtttgct aattctgccg
    18361 ctgtgtcttc tgtaagaatt gttgataata ctttttcgat tgctttattt tgtgtcgatt
    18421 ccattattat ttcctcctat taatcccttt gcccccacgg cttgcgttta tttttggtat
    18481 tcttccataa agcgatcaca ttcatcttgt gaaattctta cgactccatc aactataatc
    18541 aactttaaac cgttaggaac atatttctgc atcatagtat tgtatgaaac gttcatatac
    18601 ttagcagctt gtgccttgtt catatatttt ggaaactggt actttttttc taataatgaa
    18661 agtaacgttc cttgagtttg ctctacttgt tgaacaactc gatcgtcaat aattttaaaa
    18721 gctggatcat caagcgtgag tttgtcaatt tttaattcca tatttatccc ccctcgttag
    18781 catacatttc tatacaaaga ttttaaaaaa atgcaagagg ctcttgaata catttctata
    18841 cctcttataa actcaatata ccatcatacc ttttcatttg caagaatttt aacatacttt
    18901 tttgaacttt tatgtttcac acatatttaa atcgtgatat agtagagaaa atatatgaat
    18961 ggtggtgaaa atgtggctag aaaaaaatac cccgaagcag tggagacagg acaaagaatt
    19021 agactggcta gaattgaaaa gggactctca atggaacaac ttggaggtaa attatcccca
    19081 ccggccagta aaggtgccgt tagtaattgg gaaaatggct ataatcttcc caataacacc
    19141 aggcttaaac aactttcaaa ggtattagat gtatccacta cctatctatt aaacggatct
    19201 tatacattaa gagatatgca tatgatgccc gaaattaagc gaaatgtgct tgaaaagcaa
    19261 ttgaaaaaaa ctattgaaaa aaataactct ataattgatt ctaatctttc taatgctatc
    19321 aataacatcg aagaagataa cctgactgtc tcacagaaga tttatttaac aaacgcaata
    19381 aaatatctta aaacaggatc tcgaattgaa tttcctgacg ggagtacccc aattgcttat
    19441 ctcgcagctc ttctaaatac gcttttacga tatgactcat taattaatga ttcagaaaaa
    19501 aatacagatg agaaaaaaga gtatcaagaa ttttatgaaa atgatcttct aaaagagttc
    19561 acagctcttg ttactgagct aaataaacac tattcaaaat aataataaac tgcctcctcc
    19621 aaccgccccc acggcttgct acactatgga gggaaaacta ttatggcaac attcaagcaa
    19681 tatgaaacta aaaaaggtaa attttggctc tatgaagcct atcttggaat gaacaaaatg
    19741 actggcaaac ctgataaggt tcgtagacga ggttacaaaa caaaaaaaga agcccagctt
    19801 gccttgtcac gcttgcaagt ggactacgat aaaaacggta ttaaaaaagc gaacaacgaa
    19861 acgtttaaag aagtttatga cttgtggcta gaaacatata aaaccactgt ccgagaagcc
    19921 accttcatga aaactgaaat caagtttaga aagtggattc ttccaaaata tggaaatctt
    19981 cgtgtgaatg aagtcacggt aaaaaaagcc caacaaattg taaatagctg ggctgaaact
    20041 actgaccaat acaaggtact tcattcaact tctaagcgta tttttagcta cgctatcaat
    20101 cttgggatta ttgagactaa tccactagac aacgtattaa tgcctaaaag aaccaccact
    20161 aagcataaag aaaaggtcaa agtttacagt aaagaacaac tgatcacctt gtttgattac
    20221 ctaaacagca aacctgaaac ttatcgaaac gactttgata aaacgttact acgctttctc
    20281 ttttatagcg gggttaggat cagtgaagcc cttgcattga attggtcaga cattgatttt
    20341 caagaaaata cggtcaccat caataaaaca ctaagccaat ccaaaaatgg ctataaaatc
    20401 agtgatccta aaactgattg tagtgctggg gttcttccac tagatgaaag aacaatttct
    20461 atattaaaaa aatggcagat taatcagcgg aaatatatgc tgacacttgg tgtaactgat
    20521 ccgactatga ttttttgtgg tatctataaa gaactaatca cacaccacgc tatttatgca
    20581 agattgatta ctatttgtga aaaagctatg gttccatttc tcggtgtgca tgtagcaaga
    20641 cacactcatg cttcaatgct cttggacgct ggagctacta tggtagaagt tcaaactaga
    20701 ttacggcata caaaaattag tcagacgatc gattgttacg gtcacttggc aaaagaaacg
    20761 aaagaaaaga ctgttgaaaa attagtacaa cacttaaata ttgactagat ggcatacaat
    20821 atggcataca gtaaaaaata aaaaaaggag aaaccctatc atatcaaggt ttctcctagc
    20881 tttatagtct attcttcttc ttccgccatg aatgtattga atacttcttc aatcatgtcc
    20941 cattctgcgt ctgtttcgat ttgttctaac tcgccttctg ttccatcttc gttttcgatg
    21001 tatgcataag cttgtaattc tacatcttca tcttctgaaa caccagcagg atataaaaga
    21061 acatagttac gtccaaattc ttcttgtccg tcaactgtta gtaggatttc atataatgtt
    21121 tcatttcctt ggtcatccac caatgtgatg tgttcgtgtc cttcatggtc gtgggaatgg
    21181 ttatgttctg tcataagatt acctcttttc ttttattatt aaaataattg cttacctgag
    21241 ccgtctaaat aattttgtaa gatcatcaca gctgctaatt tatcgatgac tttttttcgt
    21301 ttggctcttg aagtatttgc ctgctctact agcatacgtt ctgcctgaac agtggttaat
    21361 cgttcatctt gataatcaac tggcaaggag aatagttctt tgatcttttc tccgtatgac
    21421 atggaagctt ctgcccttgg tccgatggaa ttgttcatat ttttaggtaa tccaactaca
    21481 aaccggtcta cttcatattg tgctactaat tccttcaacc gatcaaagcc aaactcttct
    21541 tctgcttcat tgatccgaat gatctctact ccttgagctg tccagccaaa ggggtcactg
    21601 atagcaacac ctactgtctt agaaccgaca tccaagccca ttactctcat aaattcactc
    21661 catgatttga gaggtagtcc ttgacaatta cttccatgat ctcatcgcgt tcgtgacggc
    21721 gaatcaaatt tcgagcatca cgataacgag gaatgtaggc cgggtctcca gaaagcaagt
    21781 agccgacaat ctgattgatt ggattgtagc ctttttcttc caaagcccga taaacaatcg
    21841 ctaatgtttc gctcacttct tttttgcgac tatcgtcaaa accaaaacgt acggtttcat
    21901 ctgtaaaacc catgattcta cacctctttc ccgcaaggta attccagctt accttctaca
    21961 accttatttt actcgaagaa agggaaaact acaaacaaat attccaaact tccgaataaa
    22021 ttaagaaaca ctatttgtat gtttttttcc ttttttctgt agctttatca aaagtactct
    22081 tttcataaag tatacgctat atttctttga atgtatgttt ttacacaatt ttttcataat
    22141 attcataaaa aattcgaata gctgctacta attttttgtt tttgttgctt gtcctgtctc
    22201 ataattgatc gtgtagccat cttgaacatt gtagataaag acattgaatt caattttttt
    22261 gtcttcaatg ctttctgctt ccaattgcac tcccctggct accagttcat tcccctcaaa
    22321 atgaggagtg acacggtagc gtacatgatg atttgtttct ttgatatatg ccgcaatatc
    22381 attttcgtga gcaagcatgt atggagcatt caacgaacgt gtccctgtca tcaagttttt
    22441 aatgttgtta ttttgaccag tcagctggaa accaattaaa tggctgcgat tatataacca
    22501 ttgcccgtca cttaatttct tttgtttcca acctgtcggg tcaatataca aaggctctct
    22561 cttttcagta gggaacaaat cttttccaag catagcattg gcttgtccta ctctgttcaa
    22621 tttatcaata tttgaatagc tttcccatgt tcctttggaa aggctaagat cctcttttgt
    22681 aaattccggc tgattcccat tgatttctat ttcttgatgt tttccatcat attctggcac
    22741 atccgctact tgagaagctt gtggtgtgga atcaaaaata gttgtaatct gctgaaagat
    22801 atctgaatct ttttgtgtgg tggaaacacc gaatgcccct gcaatcacag cagcgacgat
    22861 ccccacgatc gccttggttg tttttttgct catttcttta ctcctttact aacagctccc
    22921 taaatcttaa catatgttcc cctataaata aatactagaa agaaatagaa acagcaaaaa
    22981 ataaaacctg ttttaagatg gcttaaaaat acaaaaaaga gacggattcc tcgtctctaa
    23041 tcagtcacca ctctatctgt aaggtattta ttattattca aaaattttct tcatactcaa
    23101 gacaatacac tgatttcaca accgcttggt aaacggtgcg caaaagcttg catctgcggt
    23161 aataagtcca tccaagttaa aaacataaga agttgttttt gcttggctgt tcttattact
    23221 tgttgcagag cagctttttc acaacctatt ttaagcgggt gacgggaatc gaacccgcga
    23281 catcagcttg ggaagctggg attttaccac taaactacac ccgcaataca aaaacagtat
    23341 acgcctcttt tattccttcc tcaagttttt ttattcttta cgcttctggt atgttttgta
    23401 gtaaaataaa actaatcaat caattattgg agaagatgct atgacgggaa aagaattaga
    23461 tctgttgctg gacacttgtt tactcgctgg gaaaatcatg atggaaagca atgcggagat
    23521 gtatcgggta gaagatacga tgagtcgaat tgctttagct tctggaaatt tccgtttggt
    23581 cagctatgtc actcaaaccg gtctctttat tggtcttgat cgtacctcga ccatccgaat
    23641 ggaacaaatt accaaccgtt cgattaattt agaaaaagta gtaaaagtaa atgatctttc
    23701 acgtaaatat gttgcttcag agctaacatt agaagaactt tactgcgaac tgcaagcaat
    23761 cgaccgagac cgttctttct ttcctgtctg gttgcaaatc gttagcgcgg cagtgatcag
    23821 tggaacaatt atggtgttat ttggcggaaa gatgaccgat ttacctgtga ctttagtcat
    23881 tggaggattg ggatacgcag cttatctata tagtttgaaa ttttttagaa tcaaattttt
    23941 atcggaattt ttatcttctt tattgattgg catagccgct atctacagcg tacgttttgg
    24001 cttaggaagc aatcaggatc taatcattat cggatgtgtg atgcctttag ttcctggagt
    24061 tcaaattacg aacgctatcc gtgatttgct agctggacat tatgtctcag gagtttctcg
    24121 cggaacagaa gcgatgatga ctgctacgat gatcggtttt gcgattgctt ttattttcca
    24181 gcttttttat taggaaagga acacaagtat ggggacgata tttattcagt tttcttttag
    24241 ttttttagcg actgcggcat ttgccgttat tacaaatgta cctaggcgtt cattgatttg
    24301 ttgtggtctt tctggtgctt ttggctggat gatctactgg atctgtgttc aaataggcgg
    24361 atctgctgct tttggttcat tgcttggttc tttaggagta gcattcatca gcgatctttt
    24421 ctctaaacgt ttgaaaatgc ccgtaacgat tttcaatatt cccgggatgg ttcctctagt
    24481 ccctggtggt ttagcttatc aagcggttcg aaatctcgtt actgaatcct atcgagaagc
    24541 ggctcattat gcggtccaag caattatgat tgctggagca atcgctttag gtcttgtatt
    24601 atcagaagtc tttaaccata acatccgtaa tttcaaagta aagcgtgacg caatcatgat
    24661 gaaacgaaaa aaacggcttt ttaaataaac gaagtgtaac aaagaaaagg ttgtgaaaga
    24721 gacgttcagc cttgagcgat gacaaaatat ctcacaaaca ttgcgacaag taaccgcagg
    24781 agcaataaga agtaattatg agtattaaaa aaagttttgt aaaaatagct tctcacattt
    24841 ttacataaaa ttgacttaat actgagaaac tgacttttga acaccctcta tctgtaaaaa
    24901 aggggctgcg acatgatttt tgtcacggcc cctcttcttt attcaaattt tttccaaaac
    24961 gttcgttgat aataaacgat ccatagagcg gaaagtatta gaactaaacc gatcacagga
    25021 atattcacta accaaaatgg ataaaacaaa gccaacattc cgtataagac gataagaata
    25081 atactgatga tcaatgaacc catcatagtt gcgactaaac ccaaacttcc tgaacctctg
    25141 ttaaataatt ggttaatatt ggtccagttc agcagcagca accgataatc tctagcgaaa
    25201 taacgtagtg acaataagta gcagccgaat aaagaaccaa atagcaagct agggataaaa
    25261 aacaaaggca gctgaaaaga cagtccgctt aataatgcga tcccaccagt taaaaaactc
    25321 tgcaatatcc atccgaatct gaacttttct tttaaatatt ggttcataga gattggcaaa
    25381 gaacggataa acagaaaatt ttcttgatcc aaagaaataa gattgctgat aaaagaagtc
    25441 tgattaactg ttaagaaagc caatgcgatc cctaccagga aaacaacacc gattaactta
    25501 ttatctaaat cagttaattt tatttcgcct cctagggcaa aagcaagaat aaaaatgatt
    25561 ggagttaaca aggaagaaga aagaacttgc ataatcaaat taggttcttt tactaattga
    25621 gcattgtaac taaataaaag ctgatgcaca ttttggttct ttttatgggt tctttttatt
    25681 ttgaactcac tgtttgaatc agtcagttgc tctgtaagct ttggtactat cagctgtttt
    25741 acagcaagga aaaatcccag agccgtgata gctagtattc cccaacccaa taaaccttgt
    25801 tgagaaagtg gctgactggc agcatcatag aatggcaata atatcgaaat cggatgccga
    25861 tctgttaact cattttcaat agtccccgtt tgatgattca tccacatgat tccagcaaat
    25921 gcgatcagca tcgagctgcc aagcaagata ctagaaactg tttttctatg ttttttaaac
    25981 actgctgttc gagccaaacc aaaaacaatc aaactagcca gacaaaaaag aataatcaaa
    26041 aatagagaaa acaaaaggat tgagataatg atagtaagca aaatccctag atcagctcgc
    26101 catccggtta aaataaacat gactagaagc ggaaaaacaa aaggaatgat ggtcaaagca
    26161 actaccaaaa tctttgctga aaaaacctgt tcttgtccaa aaggcagagg caaaaatact
    26221 tgcaggtcat ttccttcaaa aaagatatta taaatgactg atatactttg agttaaacct
    26281 aacataccaa aaagtgccac gtaataagta aagaaacctg gcaatttaac aaagttcata
    26341 gcaaacatag caaatccgta aatcagtaaa aaaatcagcc ctgaaaaaag attttgagac
    26401 aaaagataga tagttaatcg ctttcccttt tttcctttct ctcttgcttt attcgtgagc
    26461 tgtggattaa catataaaag attaactcga attaattcta ataattgtcg cttatacata
    26521 cggtccatct cctgtcgctt gccttccagc cattttcaaa taaatctttt ctagcgtttc
    26581 atcaggtgag ccattcaata attcactaac tgccccctga taaatcaatt cacctttctt
    26641 caaaatagcc agtttatcgc ataactgttg tgcggtatcc aatacatgtg tagaaaaaat
    26701 gactgttttt ccttttgcgg catgagcctt catcatttct ttcaaatcaa atgctgcttg
    26761 aggatcaagt ccttgcaaag gttcatccaa tacccaaata tctggatctg gcagcaacgt
    26821 gccgatcaaa atcgtttttt gacgcattcc atgtgaaaaa cttgcgattg tttcattttg
    26881 atgactatac atatcaaaaa gcgctgtcaa ttctgctaaa cgcttttctt ttttttgtgt
    26941 gtctaattca taggcagctg aaatcaagtc ccaatattct ccagccgtca attgtaaaaa
    27001 aatatctggg gaatcaggca cataaccgat tttttgtttg atactcaatc gattttccgt
    27061 caatttcatt ccatctacta aaatcgttcc cgatgatggt tcaataatac taactaaact
    27121 tttaatcgta gttgattttc cggcaccatt atgtcctaaa aatccaaaaa tttccccttg
    27181 tcttatcgag agagacaagt cttttaatgc ttgttttttc ccgtaacttt tacttacatt
    27241 gatgaattca atcatttctg tactcctttt gtttgttatt actttttatt gtacccttcg
    27301 ttattggtgg agtaaatgtc aaaaagagtg cataacaaaa aaggatagaa acgactaggc
    27361 tgctacttgg gttgttctct tctacaggaa aacactcttc taacaggtaa gatatgagtc
    27421 tttgttttct ttaaaatgat tcattttcta tcttttcttg cataaaattc tttagtttat
    27481 caatacttct ctttcccatc tctagttgtt ctccatttgc aaatgttact atttcgttta
    27541 ctctgctaat agatgtaatg ttttcagtat taatgatata agcttttaaa tctttacata
    27601 aatactcata ttcgagtgat tttttatatt ttttaattgt gccgtttacg agaatctctt
    27661 catctttcaa tactaggcct attttatttt tataacctgg catgctcatt agataaagga
    27721 tttcatcgta atggatatag tgatcgctat taccataacg gacagttgtc cacctttttt
    27781 tcgtagcatg ttgaacagga tagttttctt tttctttcaa taagtaactt tggatgcttt
    27841 ttttattttt ttcataagtg aattctttta tcaaataacc actaggcgta atctttcgat
    27901 taataacagt tattgcttta ttcggatgag acgttagata aacaatttta cacttttcat
    27961 tcttttctct aagcatttcg cctagttcga taccgctata ataggcgttc aaatcgatgt
    28021 ctaggaaaaa aatatctgag gaatagattt cattattttt aatgaattca acaaattgta
    28081 taacgttcaa tccctgaacg atctcaaatt tccaaggact taataaaatt ctggattctt
    28141 ccaaaatttc tactagcatt ttttcaaaaa ccaaactatc atccaaaata aatatacgcg
    28201 ttaccataaa aagtacctcc actcgatatt ttactatcaa aatcatttca tacaactttt
    28261 caacatacat gttaggaata aattgtaatt ctcttcacaa tcttttccaa cacctttgaa
    28321 accgatatca aatactttca ggatgctgct tctaaaataa atatcaaaca aatgaataca
    28381 tcatgcaaaa aaagccagac aaccgacgtc cttgcgtgtc agctgtctga gctatgttta
    28441 tttattttgt tcttttaaaa agaacgatac acatactgat ttttcggagc agagactttc
    28501 tcagcgtttg taacaactac agatgggatt tcacgcttga atggttgata atagtctgat
    28561 tggatggttc cttctatttt ataccattca tcatttttaa ccgttactcc ttccggcata
    28621 tgcgtcaaca aaccaaagac tcctgaatct gccacacaat ggataatgcc gaatcggaac
    28681 aagaagatat ctgtattgct atcgttcggt gtttggtaaa caaatccttc atagctgatt
    28741 tttttgccaa tgaattcgct agggtaatta taaattagct ccataacttc taaatagttt
    28801 tcatctgtga tcttgatagt ttgcttatcc ccatactttt ctttcaactc attcatttgg
    28861 tctaagtagt ctgatttgtt gaaataaata ctggtatccg gctttaagta ttgtgtctgc
    28921 atctccggat cccctactga ttctttactt actgggaaat taaatccttt ggcttctacg
    28981 atcgtagtgt cgagactaac tgttggaaac aacgtcccca cgattactgg caaagctagc
    29041 agtacataag cgattccacg ttgataaggt tttgacaacc catggtcatg atcgtgctcg
    29101 tgtgcctttt cttctgcttt tccgtttttg acccacaaat acagctgaac taaagccaaa
    29161 agagcagata aaatcattga aagaatagct aagtagcgat aatggacatt gatatattga
    29221 tcgagtttgc cagaaacttg caagtacatc atcaattcga aatacccaga taaaatcaaa
    29281 aaccgcagca tattttccct cctaaataaa taatgtgaat ccaataacgc taagggcaat
    29341 cagtccgatc agcgataaaa taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt
    29401 tttgatatcg accattgggc caaagaccaa aaaggcagct acaggtgctg tgccgaacag
    29461 acttaacaaa gaagatccta taaacgcatc tgcttctgag caaagagaca tcgtaaaggc
    29521 caataacagc atgatgataa tggctaaaat ctttgttgag ccgagcgtta gcataattct
    29581 agttggtaaa taagtctgca tcgcagctgc gattaatccg ccaatgatca aataacgacc
    29641 tgtatcaaaa aattcgtcga ttgaatgtgt caacacatgc ccagtttgat ggatcagctg
    29701 attttttggt tcattcttct cactttgttc ttcttcatgg acatgatgat ggtgattttc
    29761 tgctacttta gacgtgattt tttctttcaa tacagcgtct ttttgaaaat aggcaagcca
    29821 gacaccaatc acaagggcaa caaaaaagct gcctatcacc cgccagcttg ccatctgcca
    29881 agaattgcca aatgcgataa aagtggaaaa aatcacgatt ggattgatga tcggagcagt
    29941 cagcataaaa gcaaacgctg tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat
    30001 cggtacgatg ccgcattcac atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat
    30061 agagaagata cgattttcag gaagccatct cttcacacgt tctggggtca aaaatacttg
    30121 taaggcgcct gagataagac agcctaataa tacgaaaggt aatgcttcaa taacgatcga
    30181 taagaaaatc gtactcatct gcaaaaccga atttggaata aaagaaaaca tgagtttcct
    30241 cctttttatt tctagttcat tcgctttgca aatatacgct tttattcaaa aaaaacaagt
    30301 tgctctgtaa atttttacac aactcttatt ttttaccaaa gcacttcaat tgactttttc
    30361 tgttattccc attaaattaa ggtctcatac atatcacttc tatcataaaa gggagaataa
    30421 aaatgaaaca aaccactaac ttgactgaag ttcaagacat actgcaacag tcatccgttg
    30481 ccgtcattta tctttcgatg cctaattgca gtgtttgtca cgctgttcgt ccacgactag
    30541 aggaacttct cacacattat gatattccgg cacttcattt ggatgcattt gaaacaccag
    30601 aagtagctag taaatttgaa gtgctgactg cgccagttgt cctgattttt catcaaggaa
    30661 aagaagtctt tcggcaagca cggttcatcg actttaaaaa aatccgttat ctactagatg
    30721 aactgacagc agaagatgat tccctttctt atgaagaaat cttccgaaca aaataatcgt
    30781 tgtccaaaca gaaaaaagcg attgaacaag tgaaagtttt gccttcattt gttcaatcgc
    30841 tttttatttt tattctgctg tattttcgga atgcgcattt gcgtcagaat gcaagaaata
    30901 gatcaaggtt tgcaattcat tcgtcaaatc aacattttgg atcaatacat ctgacggagc
    30961 attcaatcga gcagcggtaa agttcaagat tcctttgatt cctgcttccg ttaactgatt
    31021 gactacttct tgtgctcttt cagcgggtat cgttaaaatt gctacttcga tttgttggat
    31081 cttgatttgt tctgacatat cttccattgg ataaacgggg ataccgtcta cgatgcgccc
    31141 aacgatttcc tcattcacat caaaagcaca actgatacgt atgctattac tttgatggaa
    31201 tttgtatttt agtaaagcac ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa
    31261 ttgatcgtcg tttaatgtct ttgcaaaaaa gtgcataaga tcttctacat cataaccata
    31321 cccacgtttt cctaattcgc caaaataaga aaaatctcga cggatcgttg cactatctac
    31381 ttgaacggct tcacttaatt cagttgaaga cacttttttc tttccagcat tatttaaaat
    31441 ccttaaatag cgataataca aaggcagtct tttagctgtc gcctttggaa taatatgatc
    31501 tttcacgaaa tgacctccac gtattgtcta attacatctt tgatttctcc atcatagcag
    31561 tatttatagg aaaaaatcaa ttccattgct cagatattgt gaaattttat tctttattca
    31621 tttatttatc atttctgcaa atctcgcagt ataatggaat ttatggtaga ctactaattg
    31681 aaaaatagtt ttttatttga ggagaattca caaagtagaa aagaggattc catgatatta
    31741 ttacaagcca atcagattgc tcgccatttt ggtgcagaag tcttatttga aaacatacat
    31801 ttagaaatca gtacaggcgc tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg
    31861 actttattaa agatcattgc tggaattgaa gcacctgatg aaggaaagat cgctaaaaat
    31921 aaaacagcga ctctcggata cctcgctcag gacacaggac ttgattccaa tgaaacagtt
    31981 tggaacgaga tgctgaaagc ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag
    32041 ctagagattt tcattagcga aggtacgcca gacacacctg cttatagttc ttatttaaaa
    32101 gaatacgacc ggctgcaaca cgatttttca gatcgaaacg gctatggtta tgaaaatgaa
    32161 attcgttccg tattgcatgg gtttaaattc gattcttcat tttatgatca gaaaatcgat
    32221 actttatcag gcggacagcg cacacgttta gctttagctc gcatgctttt gcaacaccct
    32281 gacattttga ttctagatga accaacgaac catttagata tcgagacact ttcctggctt
    32341 gagaattatc tgcaaagcta tactggtgca ttattgattg tttcccatga ccgttatttt
    32401 ttagataaag tagtcaatga agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa
    32461 ggtaactatt cgaaatatct agatttgaaa gcagaacagt tagcaagtga atggaaggct
    32521 tatgaaaagc agcaaacaga aatccataaa ttagaagact tcgttgctcg gaaccttgtt
    32581 cgcgcctcta ccacaaaacg agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg
    32641 ctggatcgtc ctcaagggga tgaaaaacaa gcaaactttc tattcagtat agaaaagcct
    32701 tctggaaatg tagtcttgca aatcgaagat gccgctatcg gatatgataa tgtcacctta
    32761 tctgaaccaa tcaatctaga tatccgtcgt caagaagcga ttgctctagt aggtccgaat
    32821 ggtatcggga aatcgacgtt gctgaaatca atcattcacc agatcccttt tatccgtgga
    32881 aaagaaacgc tagggacaaa tgtcaccatc ggctattacg atcaaggtca agctgatctg
    32941 catacaaaca agacgatcct agcagaattg tgggatgagc atcctaccac tccagaaaaa
    33001 gagatccgaa gtgctttagg tgctttctta ttttctggcg aagatgtaga aaaaccaatc
    33061 cctctgctaa gtggcggtga aaaagcacgg gttgctttag ccaagttagc gatgaacaaa
    33121 gaaaattttt tgattttgga tgaaccaaca aaccatttag atattgacaa caaagaagta
    33181 ttagaaaatg cattgatcga ttatgacggc acactgcttt ttgtttccca tgaccgatat
    33241 ttcatcaatc ggatcgcgac aaaagtcgtc gaactctctg aaaatggcag caaagtctat
    33301 ctaggagact atgattatta tttagagaaa aagcaggaag aagaagaaat cgccgaattg
    33361 ttggcacaag atgagccaga atctgctcct gctcccacga acaaatctga ttattaccaa
    33421 agcaaagaac agcaaaagct tatccgtagc cttcaacgaa aaattactca ggttgaagaa
    33481 gaaatggcgc ggatcgatga atggatcgat cagttggaac aagaaatggt cgcgccagaa
    33541 aacctggaag atcacgtaaa actcaatgag ctgaaccaag atttagaagc tgctagacaa
    33601 gatcaagaaa caaaattgac ggaatgggaa gaactaagct tggaattaga agaatatgaa
    33661 aattaacgaa aaaaccaaaa cagaaaccat tcatgctgtt ttggtttttc tttttttatt
    33721 ttatcaaaca aaaattccat atttaccttt ttcaccaata agaagaccaa ttactagtca
    33781 ttctcactaa agcttctaaa taatagtaat caccccaaat acaaggtgta tttaccccat
    33841 tatttttggg cttattataa acagactgtg ttaaaaagcc atcttcttct tccgttgtag
    33901 catatctttc tttcaattgt cgcataattt tccaaacagc agactggtat aagttctttt
    33961 ctgttttgtc taatagacct gattctatta attctaataa tccacaaacc atgatcacca
    34021 tactagatgt atctttttct tctctgctag aatcatcaaa gatcaaatcc caataacaaa
    34081 tggtatcttc tggcaaccga ttcataaaat aattggctaa tattttgctt aaatcaaaat
    34141 atttttgttc tttcaaataa cgataactta atgcaaaacc gtaaatcccc catgcttggc
    34201 ctctagccca acaggaatta tcactataac cttgatgcgt acttccatat cttggttttc
    34261 catctttgac atccatataa aatgtatgat aagttgaacc atcttctctt actaaataat
    34321 ctgctgcatt ttgaatatga gcctgagcag cttttttata tttttgattt ccagtttctt
    34381 cactcgccca atacaataat ggaagattca tgcaacaatc catgatcatt ctaccttgtt
    34441 gtgaaggatc actaggatct ccccaagctt gtataactcc tgccttctcc caaaaacgct
    34501 gaagtaatgt ttctgcagca tcaattgaag cttccttcat ttttcgattt cccgttattt
    34561 tataatctgc tacggttgat aaagaataca aaaaaccaag atcgtgatga ttagtgataa
    34621 tttgacagtc aatcctgtgg ataaaatcta tatctgcttt ttcagctgtt tgttgaaata
    34681 aaggatcacc cgtcttctca aataacaacc aattaattcc tgtccaaaat ccttctgtcc
    34741 aaccaatatt gtctcttaat ggataacgtc cttttatagg acactcgctt gggaattgat
    34801 actgactgtt actgatacgt ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct
    34861 cattattcca ttctttttcc attaattctt tataacattc atttatccat ttttctggat
    34921 gtattatctc ttcaaacatc actttatcaa gcatttcccc accagccttt agcataattt
    34981 tagtcttttt ttattgtttt tattttggat agttagcccg gttgtcccat caaaatcaat
    35041 tttgggacga ttgttccata cttctaaact ataatcccga tcttgatgag caaccactcc
    35101 tgttccgagc caatgggttc cttttccaaa tgtaccctct aatccaggaa taaaagtgtt
    35161 ttcccatgtc atcaagtttg tattgggcaa acccataata gctgtggatt ttcgtttaat
    35221 agtatcgtca cttaaacaga taatacctga aaagccagct tcactcaccc tatagttttc
    35281 cttgtcttct tctatgatta ttccttcttt ctcaggagaa cattcaatag caaaccctgt
    35341 ctcatataga acatattctg tatctaattc tactttgtgt acgcgaatct gccagccttc
    35401 aaaaggaata agccaagttc tgatttttgc ctgtaaagta ggttcccatg tagaggcaag
    35461 tacctctttc ttacatacaa tgtctcgtac attatttcga acgatgaact gtccataggt
    35521 tttgatgctc tcattcttaa aactggtcgt gatatatgtg tccgctcgct gaatccctag
    35581 cattgaatcc attgccattt ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa
    35641 ttgactagaa taagcaaatt ttaaatattt ttcattggtc aatgtatgga aatttggccc
    35701 taattgtccc gcattcaaaa aaaatgtatg tccattatca tgttgtatga gcaaattggc
    35761 ttctgaaaga agtttagttc cttcacgaat aggcataggc atttctttag ccgtccaata
    35821 ctcatggtct tcaggtagcg ccaacaataa aaaaatctta ttcagccata atggagataa
    35881 cgtactatta tagggttcag tcaatgtcaa ttgagggtag gtataaccaa tattcaggat
    35941 acctttttca tcaaatattt ctttttgcat ccaccatctt atattccgat tgactaaccc
    36001 cttgatttca cctaatttaa aaggcaaaag atcggcccac aacatagctg accaaaagct
    36061 tgaaactgca aaacgataaa tcatgcttcg accaaaaggc acatttgcac catcttccgc
    36121 aaaaaaatga atatagtctc tagaaaaatc aatagctcgg ttcttatata tctggctaat
    36181 gggcttttct ggttctagta cgctataaat caaaccataa taatgaaatg caaaaggatt
    36241 ataataatct tggcgacctc ttgaactgtc tcgataccag ccattttgaa gataacaatt
    36301 ttctatatta tcaaatgctt ctttcagtgc tgattcttta actggataac caaatttttg
    36361 gataattgtt ccgacaatta tcttaaaaaa ttgccaatta ccatctgcac attcatattc
    36421 aaaaatttgc gagaaccaat gaataatctg gcttttttct cctgtatcta attttttcca
    36481 taataattgt tcatgatgat ataacgctaa taacattgcg ggcatttcta ccatacgctg
    36541 gtctctttca tgtatttctc cccaataatc gggatgggaa ggatctgttc cagcttttat
    36601 tcctgataga attaatttca accatcgttc atctaattgg tcaattgcgg gccccgctcc
    36661 ccataaaacc cgagagaatc cttccattcc tgcaatttta tttccatatc ctaccccttg
    36721 atgtatataa tcaatttgtg cgccacttgc tcgataaaac ggctggagtg gttgatttat
    36781 ctttttaaat aagtcaaagt aatcctgtct ggtccttaat agtggtactg gatgtttcat
    36841 tgtattttct cctcttttta ctaccaaata gctaattccg actgttctaa tcgaagtaag
    36901 ccttcaataa aaaaataatc agaatagatc agtggcacat tataagtcag ttctctatgg
    36961 tatgcttctg ctccgcctcc aatgatccca tcttcatcca tattccaatt tgcatagtgc
    37021 ttttctgtct tttggagtat attgatagca gcttcatgat acatttttga tcgtacagag
    37081 gaaacatatc ctgctaattc cagaagaccg catgcagcgc atactccagc actggtatca
    37141 atattcgaat cttttacagc aaaatcaatg agaggaatat aaccagtttt ttcgattttc
    37201 tgaataaaat ttactgctat cttttctgct atagctagat agtacggatt ttttgtgtaa
    37261 cgataactta acgcgaatcc atataaagcc catgcatttc ctctgctcca acaagaattc
    37321 tcattgtatc cttgcccgcc aagtaaccga atatatttcc ctgattctgc atcaaattct
    37381 gcgatatgcc cggttgatcc atctttcctc actagatact tagctgttgt atctgcatgc
    37441 tgccgagcaa ttttagagaa cctcggatca ccagtttccc tcgaagccca atacagtaat
    37501 ggtaaattca tcatactatc aataatcatc caaccaggtt tatggtcatt ccatgctact
    37561 aaatagttcc ctgaaatatt atagcgtcca gcaagtaccg tagctgcatg taaccctagt
    37621 tgtcgactat gtttgttttg agactgttga taatgtgcaa tagccgatgg taagaataaa
    37681 aaacctacat catgatgaac gtctgaaaaa tggtacaggg cagattctaa taattgttct
    37741 tgttgtattg ctgcctcttt gaacttttct cgttttgtct tgtgaaagag ctgccataaa
    37801 ataccactcc aaaaaccatt ggtccaccag tcgattcctc tgtcctttat tacgtctgta
    37861 tattttccgt tgtaagaaat atatggaatt ttattcccca ttctgtctaa ttccgcatct
    37921 atcttttcag aagctacatt caaaattttt cttgaccatt ccttcgtctg ttcattcatg
    37981 gatatcccgc cattctttta atttttatga atgtggagtt gctcctagaa cgaactagat
    38041 ggttaacatt cgttattttt tttacttatt tgcagctcca atgtcattct ccttaaattt
    38101 agactgcgcc cacacataaa tcaagaccat tgggatcact gaagttgcta aaattagcat
    38161 aattgcgcca taattaattg gctcacctgt actagaaacc gtcatatttt tgataattaa
    38221 gggaaccgta tatttatctt tatctgaaag catgactaat ggaaatagat attgattcca
    38281 acttcccata aagataagta aaccactagt aataatcgta ggtgataaaa tcggtacagc
    38341 gattttcgta aaaatcaccc actcattggc accatcgatt ctggcagcct ccatcagtgc
    38401 tgtaggaaat tgactgctat attgtcgcat cataaacact gaagttgtac tggcaatcga
    38461 agggatgatc acacctgcat aagtattcga taatcctaaa cgactcataa tgtaaaataa
    38521 aggaatcaat gtggtaaaaa aaggcaccat cctagatagc ataactgtat tgaacaacag
    38581 tttattcccc ataaaacgat actttgctaa tgcataaccc gccattgtag aactggctgt
    38641 agctaaaact gtactgatta cagcaataac aatcgaattc caaataatca agggataatt
    38701 atatctcgcc atgatggctt gccagttttt taatagcata tccccaaact tgaaattgat
    38761 tccgccactt aaaatttctt gattactttg cgttgaagct acaatcatat ataaatatgg
    38821 catcaaaaat attaaggcac caaacacaat aatgacgtac gtaaaaattc tgctttgtat
    38881 cttttgttta gtcattttta tcacctccga atttaaattg aatagccgat aatataacag
    38941 atacaaacac catcgtccag gcaatagcag aggcttttcc aaaatcaacc atttcaaaac
    39001 ttgttttata taaattgact cctacgacca aagctgaatt ttctggtcca ttggaagtcc
    39061 caaagaatat atttgggata ttgaaattac ccaagccatt gatagtggct aaaactgtgg
    39121 ccattaaaat gattggcttt agcatgggga tcgttaataa aaagaattgc cgaaatttcg
    39181 atgctccatc tatttcagca ctttcgtata tctctttaga aatcccttga agaccagcta
    39241 aaaataaaat agtgttatat cctacactaa gccaaatcat cgtgatgatc attgatattc
    39301 gtatccaaaa aggatttgtt aaccactgta caccgttaat tcctagcgca ttcaaaaatc
    39361 cattgaataa cccagtcgga ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg
    39421 atgtaatggt tggaatataa tagacagttc taaaaaatcc ttttcctttt aactgtgctg
    39481 aattcaataa aacagcaaag taaatagcta atagcatcgt tattggaatt gatcctgcca
    39541 tgataatcaa aacattcgtg taagatttaa taaacaatgg atctcggaag atagctagat
    39601 aattgtcaaa tccaacaaat tcaaatgagt agccaatttt cataaaacta aaagcaaacg
    39661 agataatgat gggaatcaaa atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc
    39721 cataagcact ttttttatga gacgtatctg ttgctttttt cttgctgttc tttattgtct
    39781 tgcttggtaa cgaagattgc atcatcgtta agtcctccta atatttttta atcaccagtt
    39841 aattttacgc cgtacttatg agcaaaattt tctgcttctt ttagatatga attttcaaaa
    39901 tcacctgttt gccaatagtt atatgcagcg gtagtcaaat aagacgtagc atctgaataa
    39961 gtggctggta aaacagtatt tgatgtattt tcattgattt tattcaatag ttcatagtat
    40021 ttttgtcctc cttagtagtc aaactttttc tcagctgctt ctgtttgata ggccaatgag
    40081 ttagatgtag ccacgcccag ctccagtgcg ttggctaagc attcttcatc tgtcagcata
    40141 aatgtaagaa attgttgtgc cacttgtgga tttttggcat ctttagggac ataaaaactt
    40201 gatcctcctg atactgcacc ttttcctgga ttttcttttg aaaaaggaat cagtggagcc
    40261 atgcgccatt tgtcagatgc atcaggaaaa ttcaaagaca tattcgtagc ataccagccg
    40321 ccagcaacaa acattgcaga ttcttgaaat gctttctcag catcttgtgc accgtagaaa
    40381 tgtacaatat ctgagtcgat catttctttg attaactttg cagcatgttt cgcttcttta
    40441 gtattcagac ggatatttcc ttcttcatca agcaaagatt gattctgctg ggctagcatg
    40501 ttgataaaca attcttggtc cccagattct ggcaaggcaa ttccaaaact accagttttt
    40561 tcttttaatg cttttgccac ctggatatat tcttcccaag aactgatttc ttcaaaatct
    40621 acacctattt cagcaaattt ttttggttga taataacaaa gtgctggggt aaaatcacta
    40681 gcccaaggaa taatcgtttc attactcgtt tgttgacgca aaatattcaa tttttgatca
    40741 taaaactgat cgccatattt gtcataaaat ccatattcat cagcactata aaaagcatct
    40801 tgaaattttt ccagcacacc tgctgctgaa gtgtctgcca tggagataat atccggcata
    40861 tcttcgtgtg ttaccaattt tggggtgatc atttgtactg ttgctgttcc cgaagctaca
    40921 ttcacaattt ttaagtcaat atttgctcca gttttgcggt tatagatttt tgcagcattt
    40981 tcaacatagt ttgtcagttt gccgtttgcg acatatactt ctaatgtagt ggcttttttt
    41041 atcattggat ctgcgatgtt tgctgttact tcttcgttag tttttccaca tgcacttaag
    41101 ctgaataata gggtaatgtt tagcaaagct atcaccaatt tgtatctttt tttcatgtct
    41161 atcccttctt tcttttcaag ttcttatata aaacgctttc acttatgtat attaacggaa
    41221 ttagtaacac aaatatttat ataaaaaaat attttacttg tcgttttggc ttttcatttt
    41281 tgttataata gtcattaatt ataaatagaa agctttttat ataaaaagag aaaggaaagt
    41341 ggttcttttg ttaacaaaca accaatcctc caatcttgtc aaaaaaacac taacctattc
    41401 catagatacc catcttcatt tatctgctgc tccctatttt cgactactaa taaatgaaag
    41461 cgctaattta tatgctgaat ttgcacataa atctatagaa ctagataatc cttccctttt
    41521 attcattgat aaacagcaag aagtccaaat cttttctact caaaaggaaa cacaaacttt
    41581 tatcattatc cagttttatt ttgatgagca attattagca catattacgg gacaatcttt
    41641 ttctatagat caagaaataa catctatcat tacttttctg aaaaatgaac agttcaaaat
    41701 tgattatcta aaacatagta agccagctat ttcctatgat caggaaatca attctttttt
    41761 catactatgc caacagatga gcattcattt gctttcctta ttagttactt attgtctgca
    41821 aaaaattcaa ttgtcttcct taccaaggca ttatcaacag gaacttgctt tattgacaat
    41881 accttcaaat gagaaagtca ttcctaaaaa aaagcatatt ctttattcag atgaattagt
    41941 tgatccaatc atacgatatt tagaagaaca tctttcagaa aattttttgc ttgaacaatt
    42001 agcccaaatt ttttttatca gtccagccag cttaaaaaaa cattttagaa aagtgaatgg
    42061 aaaaagtgtt attagctatt ttaaagaact aaaaatggaa aaagcaaaac gtctcgtgct
    42121 agaaaatgaa ttaacctata cagaaattgc agatatatta ggatttcatt cgatccatca
    42181 tttttcttca gcttttaaaa aatatacagg gttatcccct tcaaagtatt atctatcctt
    42241 aaaatagaaa aaaattttta ggttttatcc acaaattatt tctctatatt ttctgtctat
    42301 tcaaaataaa caataatttt ccggcacaat tatttgctgt tttttaccat aacatagtac
    42361 gatcaacgta gaaaaggaga tgtttttatg aaagttgttg tcattggcgg tgtagctggc
    42421 ggtccttcat ttgccactcg tttccgtcga ttgaatgaag cgcacgaaat cattatctat
    42481 gaacgcggag aaaatatttc ttatgcaagt tgtgctttgc cttattattt aggaggtgtg
    42541 atcactgacc gtgactcgct gatcgaacgt acaccagaaa tattgaaaac aaaaaacaac
    42601 atcgatgtct ttactaaaca cgaagtaaca gcaatcgatc cttctactaa gcgattaaca
    42661 gttaaagacc tatccacaaa tgaagaaata aaaacagatt acgataagtt gatcatctct
    42721 tctggtgcta gaccagatta tccggatatt cccggagttt ttgaagcaga aaacggcttt
    42781 gtcctccgta gtgtgacgga tgcggatcga atcaaatcgt tccttgaaga aaaaaatcca
    42841 caacatgtcg tcattcttgg tgcaggtgtt atgggtctgg aattagctga gaatttcaag
    42901 catcgcggct tagacgtgac tttaatcgat caattgccac aagtcgcttt cccttatgat
    42961 ccagaaattg ctaatttagt ttatgacaaa ttgctgaaag aaggattagc cgttcattta
    43021 gaaacaagag ttactgagat ccgtgataaa ggtcgagaaa tagtattatc agatggctcc
    43081 gtcctttctg ctgatatgct aatttttgct gttggtgttt ctccgaagaa tgaagtggtg
    43141 aaagcaaccg gcatacaatt atcagataca ggtcagatca ttgttgatga ccagttacaa
    43201 accaatctgc cggacatcta tgcgattggc gatattatcg aaacgactag tgtagtgact
    43261 ggtcagccga tccaaagtat gctttccagt gcggccaatc gtcaaggaca tatgctggca
    43321 gatattttaa atggtacgcc tatgcgctat cgcggatata ttggtgcagg tgtcgcaaaa
    43381 atctttgatc atacagcaag ttatgcggga atgacagaac atgaattaaa agcatcaggc
    43441 ataacaaatt ataaaactgt ttttatcact ccctttgacc atgcctattt ctatccagaa
    43501 gctacaagat taaatctaaa gctgattttt gatgcagata gcggtcgtat tttaggtgga
    43561 caagcatttg gagaaaaagg tgtcgataaa cggatgggag aactttctgt agcaatcacc
    43621 ggaaacttga cagtctttga tttgcctgat ttggagttgc cttactctcc accgtattct
    43681 actacccgtg acccgttgaa tatagctggt tatgtcgcaa tcaatcaaat gacgaatatc
    43741 gtagaaacga tcaaagcaag tgatataccc gaaaacgatt tgaaagaagc gttcttttta
    43801 gatatacgtg aacctaataa agcaccttct ggcagtatct cggcaacaaa aaatattcca
    43861 atgaatgagt tacgagatcg aatcaatgaa atcccaaaag ataaaaaagt ttacattact
    43921 ttcagaagag gattgaatac ttatacttct gcccgaatct tggcaggttt gggtatcaaa
    43981 gcgattttga ttgaagaata aaaaaacgac cgcggtgggt agccgcggtc taggagttat
    44041 ttttacttga ggagtaaaaa tgaaaaagta tatagggttg ttggttggta tgtatataga
    44101 atagaagata aatgtgaaga aattgtgcat tatttcttca catttattag gttatagttt
    44161 aagaaagttt aaacattgcg caatttaatt attttagctg tttcttttca taaaaaaaca
    44221 tgttgtgact ctgagacaaa tcatagtcca tagtgatgtc tctagagtaa ctacgcctaa
    44281 tattacgagg catcatatta atctatcagc actagtacct aaactagcat ccctttcgaa
    44341 tattttttct tatataataa agtttgcttt cactcctcta atctcaccaa atcgactggc
    44401 ttgcgttgtg tgatactagg aactgattgg tttagaacga cttttctatt gtaatcatat
    44461 ttttcgccat cctcgttgtc ttctaatcct tcaatgaatg atccctaatg tgtgtattaa
    44521 ataatttaat ttttttgttc ctatttattt aa
//
LOCUS       DASJPC010000013        43749 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJPC010000013 DASJPC010000000
VERSION     DASJPC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.013"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..1634)
                     /gene="guaA"
                     /locus_tag="U4A13_000684"
     CDS             complement(69..1634)
                     /gene="guaA"
                     /locus_tag="U4A13_000684"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1779005.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1693..2616)
                     /locus_tag="U4A13_000685"
     CDS             complement(1693..2616)
                     /locus_tag="U4A13_000685"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN1779006.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2812..3417
                     /locus_tag="U4A13_000686"
     CDS             2812..3417
                     /locus_tag="U4A13_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1779007.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3725..4741
                     /locus_tag="U4A13_000687"
     CDS             3725..4741
                     /locus_tag="U4A13_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1779008.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4882..6183
                     /locus_tag="U4A13_000688"
     CDS             4882..6183
                     /locus_tag="U4A13_000688"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN1779009.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6229..6891
                     /gene="deoC"
                     /locus_tag="U4A13_000689"
     CDS             6229..6891
                     /gene="deoC"
                     /locus_tag="U4A13_000689"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN1779010.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6912..7310
                     /locus_tag="U4A13_000690"
     CDS             6912..7310
                     /locus_tag="U4A13_000690"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN1779011.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7376..8458
                     /locus_tag="U4A13_000691"
     CDS             7376..8458
                     /locus_tag="U4A13_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1779012.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8670..10232
                     /locus_tag="U4A13_000692"
     CDS             8670..10232
                     /locus_tag="U4A13_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779013.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10225..11358
                     /locus_tag="U4A13_000693"
     CDS             10225..11358
                     /locus_tag="U4A13_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779014.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11358..12314
                     /locus_tag="U4A13_000694"
     CDS             11358..12314
                     /locus_tag="U4A13_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779015.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12413..13579
                     /gene="deoB"
                     /locus_tag="U4A13_000695"
     CDS             12413..13579
                     /gene="deoB"
                     /locus_tag="U4A13_000695"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN1779016.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13603..14421
                     /locus_tag="U4A13_000696"
     CDS             13603..14421
                     /locus_tag="U4A13_000696"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1779017.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14438..15145
                     /gene="deoD"
                     /locus_tag="U4A13_000697"
     CDS             14438..15145
                     /gene="deoD"
                     /locus_tag="U4A13_000697"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN1779018.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15443..16129)
                     /gene="gpmA"
                     /locus_tag="U4A13_000698"
     CDS             complement(15443..16129)
                     /gene="gpmA"
                     /locus_tag="U4A13_000698"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1779019.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16301..16882)
                     /locus_tag="U4A13_000699"
     CDS             complement(16301..16882)
                     /locus_tag="U4A13_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1779020.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(17015..17698)
                     /gene="rpiA"
                     /locus_tag="U4A13_000700"
     CDS             complement(17015..17698)
                     /gene="rpiA"
                     /locus_tag="U4A13_000700"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN1779021.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            18017..18430
                     /gene="rpsL"
                     /locus_tag="U4A13_000701"
     CDS             18017..18430
                     /gene="rpsL"
                     /locus_tag="U4A13_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN1779022.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18514..18984
                     /gene="rpsG"
                     /locus_tag="U4A13_000702"
     CDS             18514..18984
                     /gene="rpsG"
                     /locus_tag="U4A13_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN1779023.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19078..21165
                     /gene="fusA"
                     /locus_tag="U4A13_000703"
     CDS             19078..21165
                     /gene="fusA"
                     /locus_tag="U4A13_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1779024.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21337..22524
                     /gene="tuf"
                     /locus_tag="U4A13_000704"
     CDS             21337..22524
                     /gene="tuf"
                     /locus_tag="U4A13_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1779025.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            22931..23239
                     /gene="rpsJ"
                     /locus_tag="U4A13_000705"
     CDS             22931..23239
                     /gene="rpsJ"
                     /locus_tag="U4A13_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN1779026.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23270..23899
                     /gene="rplC"
                     /locus_tag="U4A13_000706"
     CDS             23270..23899
                     /gene="rplC"
                     /locus_tag="U4A13_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN1779027.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            23927..24550
                     /gene="rplD"
                     /locus_tag="U4A13_000707"
     CDS             23927..24550
                     /gene="rplD"
                     /locus_tag="U4A13_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN1779028.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24550..24840
                     /gene="rplW"
                     /locus_tag="U4A13_000708"
     CDS             24550..24840
                     /gene="rplW"
                     /locus_tag="U4A13_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN1779029.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24879..25712
                     /gene="rplB"
                     /locus_tag="U4A13_000709"
     CDS             24879..25712
                     /gene="rplB"
                     /locus_tag="U4A13_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN1779030.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25755..26033
                     /gene="rpsS"
                     /locus_tag="U4A13_000710"
     CDS             25755..26033
                     /gene="rpsS"
                     /locus_tag="U4A13_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN1779031.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26056..26403
                     /gene="rplV"
                     /locus_tag="U4A13_000711"
     CDS             26056..26403
                     /gene="rplV"
                     /locus_tag="U4A13_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN1779032.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26417..27073
                     /gene="rpsC"
                     /locus_tag="U4A13_000712"
     CDS             26417..27073
                     /gene="rpsC"
                     /locus_tag="U4A13_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN1779033.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27076..27510
                     /gene="rplP"
                     /locus_tag="U4A13_000713"
     CDS             27076..27510
                     /gene="rplP"
                     /locus_tag="U4A13_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN1779034.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27500..27688
                     /gene="rpmC"
                     /locus_tag="U4A13_000714"
     CDS             27500..27688
                     /gene="rpmC"
                     /locus_tag="U4A13_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN1779035.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27713..27979
                     /gene="rpsQ"
                     /locus_tag="U4A13_000715"
     CDS             27713..27979
                     /gene="rpsQ"
                     /locus_tag="U4A13_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN1779036.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            28037..28405
                     /gene="rplN"
                     /locus_tag="U4A13_000716"
     CDS             28037..28405
                     /gene="rplN"
                     /locus_tag="U4A13_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN1779037.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28442..28750
                     /gene="rplX"
                     /locus_tag="U4A13_000717"
     CDS             28442..28750
                     /gene="rplX"
                     /locus_tag="U4A13_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN1779038.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28778..29317
                     /gene="rplE"
                     /locus_tag="U4A13_000718"
     CDS             28778..29317
                     /gene="rplE"
                     /locus_tag="U4A13_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN1779039.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29336..29521
                     /locus_tag="U4A13_000719"
     CDS             29336..29521
                     /locus_tag="U4A13_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN1779040.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29557..29955
                     /gene="rpsH"
                     /locus_tag="U4A13_000720"
     CDS             29557..29955
                     /gene="rpsH"
                     /locus_tag="U4A13_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN1779041.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            29987..30523
                     /gene="rplF"
                     /locus_tag="U4A13_000721"
     CDS             29987..30523
                     /gene="rplF"
                     /locus_tag="U4A13_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN1779042.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30684..31040
                     /gene="rplR"
                     /locus_tag="U4A13_000722"
     CDS             30684..31040
                     /gene="rplR"
                     /locus_tag="U4A13_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN1779043.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31061..31561
                     /gene="rpsE"
                     /locus_tag="U4A13_000723"
     CDS             31061..31561
                     /gene="rpsE"
                     /locus_tag="U4A13_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN1779044.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31576..31755
                     /gene="rpmD"
                     /locus_tag="U4A13_000724"
     CDS             31576..31755
                     /gene="rpmD"
                     /locus_tag="U4A13_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN1779045.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31799..32239
                     /gene="rplO"
                     /locus_tag="U4A13_000725"
     CDS             31799..32239
                     /gene="rplO"
                     /locus_tag="U4A13_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN1779046.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32239..33534
                     /gene="secY"
                     /locus_tag="U4A13_000726"
     CDS             32239..33534
                     /gene="secY"
                     /locus_tag="U4A13_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN1779047.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33596..34243
                     /locus_tag="U4A13_000727"
     CDS             33596..34243
                     /locus_tag="U4A13_000727"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN1779048.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34434..34652
                     /gene="infA"
                     /locus_tag="U4A13_000728"
     CDS             34434..34652
                     /gene="infA"
                     /locus_tag="U4A13_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN1779049.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34685..34801
                     /gene="rpmJ"
                     /locus_tag="U4A13_000729"
     CDS             34685..34801
                     /gene="rpmJ"
                     /locus_tag="U4A13_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN1779050.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34819..35184
                     /gene="rpsM"
                     /locus_tag="U4A13_000730"
     CDS             34819..35184
                     /gene="rpsM"
                     /locus_tag="U4A13_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN1779051.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35212..35601
                     /gene="rpsK"
                     /locus_tag="U4A13_000731"
     CDS             35212..35601
                     /gene="rpsK"
                     /locus_tag="U4A13_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN1779052.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35681..36619
                     /locus_tag="U4A13_000732"
     CDS             35681..36619
                     /locus_tag="U4A13_000732"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN1779053.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36647..37027
                     /gene="rplQ"
                     /locus_tag="U4A13_000733"
     CDS             36647..37027
                     /gene="rplQ"
                     /locus_tag="U4A13_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN1779054.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37299..38138
                     /locus_tag="U4A13_000734"
     CDS             37299..38138
                     /locus_tag="U4A13_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1779055.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38114..38983
                     /locus_tag="U4A13_000735"
     CDS             38114..38983
                     /locus_tag="U4A13_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1779056.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            38973..39770
                     /locus_tag="U4A13_000736"
     CDS             38973..39770
                     /locus_tag="U4A13_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1779057.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            39973..40719
                     /gene="truA"
                     /locus_tag="U4A13_000737"
     CDS             39973..40719
                     /gene="truA"
                     /locus_tag="U4A13_000737"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN1779058.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(40780..40986)
                     /locus_tag="U4A13_000738"
     CDS             complement(40780..40986)
                     /locus_tag="U4A13_000738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779059.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(41052..43721)
                     /locus_tag="U4A13_000739"
     CDS             complement(41052..43721)
                     /locus_tag="U4A13_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN1779060.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        14017 a         8123 c         9491 g        12118 t
ORIGIN      
        1 aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga tttctagctc
       61 tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata
      121 cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat
      181 gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact
      241 gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca
      301 gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata
      361 gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata
      421 ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga
      481 gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat
      541 tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct
      601 gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct
      661 tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt
      721 ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca
      781 tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct
      841 tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct
      901 ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact
      961 tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt
     1021 gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg
     1081 tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct
     1141 tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact
     1201 ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac
     1261 aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca
     1321 ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat
     1381 acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca
     1441 ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca
     1501 gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc
     1561 aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg
     1621 gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac
     1681 tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt
     1741 atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa
     1801 attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc
     1861 aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg
     1921 atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa
     1981 aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac
     2041 gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc
     2101 atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc
     2161 gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc
     2221 ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg
     2281 tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc
     2341 tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa
     2401 acctttactc aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag
     2461 aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt
     2521 atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc
     2581 tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc
     2641 aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat
     2701 ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga
     2761 tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat
     2821 cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta
     2881 aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat
     2941 tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag
     3001 atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag
     3061 cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca
     3121 aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat
     3181 gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta
     3241 catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg
     3301 atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat
     3361 gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta
     3421 gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc
     3481 agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa
     3541 tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag
     3601 aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc
     3661 tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa
     3721 taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt
     3781 ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt
     3841 gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg
     3901 acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact
     3961 gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc
     4021 tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga
     4081 cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt
     4141 ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg
     4201 gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg
     4261 ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt
     4321 caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga
     4381 acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa
     4441 agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat
     4501 cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa
     4561 gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg
     4621 attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa
     4681 caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta
     4741 actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt
     4801 ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat
     4861 gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga
     4921 aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt
     4981 ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa
     5041 gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct
     5101 cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg
     5161 attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt
     5221 ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag
     5281 ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa
     5341 tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc
     5401 gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca
     5461 gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat
     5521 gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg
     5581 gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt
     5641 gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac
     5701 gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga
     5761 acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc
     5821 caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc
     5881 tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa
     5941 ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat
     6001 cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca
     6061 ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa
     6121 aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa
     6181 taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac
     6241 cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt
     6301 atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg
     6361 ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct
     6421 ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt
     6481 gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa
     6541 gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt
     6601 attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa
     6661 gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta
     6721 gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc
     6781 ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga
     6841 acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa
     6901 aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag
     6961 ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag
     7021 gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg
     7081 agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg
     7141 tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag
     7201 aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa
     7261 cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca
     7321 ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa
     7381 aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc
     7441 atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac
     7501 tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg
     7561 ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca
     7621 atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc
     7681 tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc
     7741 aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa
     7801 cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc
     7861 ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga
     7921 tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga
     7981 aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat
     8041 tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg
     8101 taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt
     8161 tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa
     8221 aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca
     8281 aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga
     8341 cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc
     8401 aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa
     8461 ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga
     8521 attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt
     8581 aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta
     8641 gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc
     8701 agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa
     8761 aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga
     8821 acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag
     8881 taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact
     8941 ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc
     9001 gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt
     9061 atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac
     9121 gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga
     9181 cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca
     9241 aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg
     9301 accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa
     9361 cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa
     9421 aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc
     9481 gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta
     9541 tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa
     9601 aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa
     9661 aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc
     9721 tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta
     9781 aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat
     9841 atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc
     9901 aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc
     9961 caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa
    10021 gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt
    10081 cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa
    10141 catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag
    10201 agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta
    10261 gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt
    10321 ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga
    10381 gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct
    10441 gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg
    10501 atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg
    10561 ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt
    10621 cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta
    10681 tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac
    10741 tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt
    10801 ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg
    10861 ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt
    10921 gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct
    10981 ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt
    11041 gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct
    11101 ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga
    11161 cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg
    11221 attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa
    11281 gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat
    11341 gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac
    11401 tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg
    11461 ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt
    11521 tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg
    11581 gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg
    11641 ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct
    11701 tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt
    11761 tttctttccc aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat
    11821 tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga
    11881 cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag
    11941 gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta
    12001 tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg
    12061 gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa
    12121 tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac
    12181 cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca
    12241 ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata
    12301 tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc
    12361 tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa
    12421 acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa
    12481 gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt
    12541 tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt
    12601 gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat
    12661 gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa
    12721 tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg
    12781 taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac
    12841 aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga
    12901 aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga
    12961 tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt
    13021 cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga
    13081 ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa
    13141 cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct
    13201 gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga
    13261 tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga
    13321 cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc
    13381 cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt
    13441 attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga
    13501 tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt
    13561 cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag
    13621 atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt
    13681 ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt
    13741 tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta
    13801 gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat
    13861 gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc
    13921 cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat
    13981 ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat
    14041 gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa
    14101 gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg
    14161 ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga
    14221 gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta
    14281 aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc
    14341 catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa
    14401 gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa
    14461 agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat
    14521 tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt
    14581 taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc
    14641 agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt
    14701 aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg
    14761 tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat
    14821 cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt
    14881 tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt
    14941 ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt
    15001 aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac
    15061 cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg
    15121 attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat
    15181 aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca
    15241 aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt
    15301 cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc
    15361 gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac
    15421 agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat
    15481 ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag
    15541 agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa
    15601 cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt
    15661 ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac
    15721 gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt
    15781 aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt
    15841 tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta
    15901 cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa
    15961 cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt
    16021 ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca
    16081 atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc
    16141 cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg
    16201 gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa
    16261 aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga
    16321 tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg
    16381 aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg
    16441 tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc
    16501 caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt
    16561 ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca
    16621 gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc
    16681 gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca
    16741 aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac
    16801 aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc
    16861 gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct aactggatat
    16921 aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct
    16981 gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa
    17041 tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat
    17101 gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc tgttcgatga
    17161 cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt
    17221 cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg
    17281 agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag
    17341 aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca
    17401 aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta
    17461 ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg
    17521 cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca
    17581 tacggcgacc aatctcttct accatataat aggcggttga accggtcccg agacctacga
    17641 tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct
    17701 ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta
    17761 cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct
    17821 cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc
    17881 tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat
    17941 ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg
    18001 aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa
    18061 ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca
    18121 aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc
    18181 taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga
    18241 agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt
    18301 acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt
    18361 agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc
    18421 taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta
    18481 ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa
    18541 cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt
    18601 gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc ctttgacttg
    18661 atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt
    18721 caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt
    18781 gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc
    18841 ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac
    18901 aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt
    18961 gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc
    19021 tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg
    19081 accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct
    19141 ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt
    19201 gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt
    19261 acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac
    19321 acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt
    19381 gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt
    19441 caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt
    19501 gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca
    19561 atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg
    19621 aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa
    19681 gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact
    19741 gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa
    19801 gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca
    19861 tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg
    19921 ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct
    19981 gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta
    20041 ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa
    20101 aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct
    20161 cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac
    20221 acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa
    20281 ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa
    20341 atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac
    20401 gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga tatcatcgtt
    20461 gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac
    20521 cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt
    20581 aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc
    20641 gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa
    20701 actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc
    20761 aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt
    20821 gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa
    20881 ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac
    20941 gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt
    21001 gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct
    21061 actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca
    21121 gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct taaaaccgga
    21181 tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata
    21241 attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat
    21301 aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt
    21361 tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta
    21421 acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat
    21481 caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt
    21541 gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac
    21601 gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct
    21661 gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt
    21721 ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa
    21781 ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt
    21841 cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa
    21901 attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac
    21961 aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct
    22021 acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt
    22081 gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac
    22141 tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc
    22201 caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca
    22261 gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac
    22321 cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga
    22381 actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc
    22441 gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt
    22501 gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg
    22561 gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt tatttattgc
    22621 aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata aaagagtcag
    22681 tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt
    22741 gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt
    22801 aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc
    22861 acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg
    22921 agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat
    22981 tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc
    23041 tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa
    23101 atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt
    23161 gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa
    23221 catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg
    23281 aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc
    23341 agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga
    23401 tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc
    23461 tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa
    23521 aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc
    23581 aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa
    23641 acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg
    23701 gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat
    23761 gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa
    23821 tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta ctatcaaatc
    23881 agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc
    23941 attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg
    24001 aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag
    24061 acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg
    24121 gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg
    24181 aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt
    24241 tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc
    24301 aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa
    24361 cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt
    24421 atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga
    24481 cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt
    24541 gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg
    24601 cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta
    24661 gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac
    24721 gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa
    24781 gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa
    24841 gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac
    24901 ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa
    24961 cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc
    25021 gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca
    25081 aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt
    25141 cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta
    25201 aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa
    25261 atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac
    25321 ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag
    25381 aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat
    25441 gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag
    25501 ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta
    25561 atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt
    25621 ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca
    25681 aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg
    25741 agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa
    25801 tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac
    25861 gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat gatggacgta
    25921 aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac
    25981 ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg
    26041 ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt
    26101 cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca
    26161 gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta
    26221 ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta
    26281 gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa
    26341 ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa
    26401 taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat
    26461 ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga
    26521 tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga
    26581 aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt
    26641 tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg
    26701 taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt
    26761 aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca
    26821 agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg
    26881 tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca
    26941 tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact
    27001 aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa
    27061 aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga
    27121 aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa
    27181 gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact
    27241 cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact
    27301 gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca
    27361 ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt
    27421 gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag
    27481 gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat
    27541 gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac
    27601 aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa
    27661 aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga
    27721 aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac
    27781 aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta
    27841 ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag
    27901 aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga
    27961 agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg caaaatctga
    28021 aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg
    28081 cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat
    28141 cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg
    28201 tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc
    28261 atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg
    28321 aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt
    28381 ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt
    28441 tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga
    28501 aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt
    28561 cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga
    28621 agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt
    28681 tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt
    28741 agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata
    28801 tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac
    28861 acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa
    28921 aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac
    28981 aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa
    29041 agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact
    29101 accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta
    29161 tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa
    29221 agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga
    29281 gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc
    29341 taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac
    29401 tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat
    29461 ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta
    29521 aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca
    29581 gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct
    29641 gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat
    29701 gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa
    29761 aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat
    29821 gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact
    29881 tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc
    29941 gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg
    30001 aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac
    30061 agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat
    30121 cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca
    30181 tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa
    30241 agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt
    30301 gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt
    30361 accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc
    30421 tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt
    30481 tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc
    30541 atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt
    30601 tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca
    30661 ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa
    30721 agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg
    30781 tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc
    30841 tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac
    30901 aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct
    30961 ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg
    31021 aaaatggact agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa
    31081 acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg
    31141 tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg
    31201 gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc
    31261 gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg
    31321 tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc
    31381 tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc
    31441 tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt
    31501 gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata
    31561 aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt
    31621 cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg
    31681 gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac
    31741 gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat
    31801 gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg
    31861 tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg
    31921 ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt
    31981 accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt
    32041 attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat
    32101 cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt
    32161 aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg
    32221 atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt
    32281 agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt
    32341 actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac
    32401 atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt
    32461 tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga
    32521 tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga
    32581 tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa
    32641 tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg
    32701 attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt
    32761 atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt
    32821 atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct
    32881 gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt
    32941 ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct
    33001 acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct
    33061 agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct
    33121 gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg
    33181 atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct
    33241 gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt
    33301 aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc
    33361 ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa
    33421 tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg
    33481 aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga gtaagaaagc
    33541 gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa
    33601 cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga
    33661 tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga
    33721 aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga
    33781 agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt
    33841 attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga
    33901 tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga
    33961 acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa
    34021 ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga
    34081 agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc
    34141 aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat
    34201 cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc
    34261 gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc
    34321 cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt
    34381 ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa
    34441 aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta
    34501 aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc
    34561 attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta
    34621 accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat
    34681 tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg
    34741 taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata
    34801 attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt
    34861 gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta
    34921 gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat
    34981 gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac
    35041 ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga
    35101 ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc
    35161 gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca
    35221 aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc
    35281 cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg
    35341 tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct
    35401 caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa
    35461 gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca
    35521 ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct
    35581 ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt
    35641 gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc
    35701 aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga
    35761 aagagggtat gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc
    35821 aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa
    35881 aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat
    35941 gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc
    36001 tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag
    36061 tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt
    36121 tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat
    36181 ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga
    36241 agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc
    36301 aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga
    36361 tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt
    36421 ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc
    36481 aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg
    36541 taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg
    36601 tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa
    36661 actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt
    36721 aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga
    36781 aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt
    36841 cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc
    36901 tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac
    36961 acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact
    37021 tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt
    37081 tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga
    37141 gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt
    37201 ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat
    37261 agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag
    37321 aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct
    37381 ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga
    37441 cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa
    37501 atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga atgatttttc
    37561 aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg
    37621 aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag
    37681 ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac
    37741 gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa
    37801 ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag
    37861 aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca
    37921 atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt
    37981 tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga
    38041 agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga
    38101 tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac
    38161 accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac
    38221 tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct
    38281 tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga
    38341 taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc
    38401 acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt
    38461 cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga
    38521 aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat
    38581 cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact
    38641 tgatccccag ggcagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca
    38701 gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt
    38761 ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa
    38821 cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga
    38881 attaatggct aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga
    38941 cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt
    39001 atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt
    39061 tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat
    39121 ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca
    39181 ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg
    39241 gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg
    39301 gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta
    39361 ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg
    39421 tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta
    39481 cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg
    39541 gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg
    39601 tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag
    39661 gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta
    39721 tcattgcttt tgtattatta actgtgctac tgatcttttt aagaggataa acgttcagtg
    39781 ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcaa gcagtgtatg
    39841 acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa
    39901 agaaagaacg aaaaaatttt atagaaaaat cattattcca gataaccttt taggaacaaa
    39961 ggagagaaaa acatggtacg ttacaaagca atcattgcgt acgacggaac aaattttaat
    40021 ggattccaaa aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa
    40081 aaaatggcaa atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta
    40141 catgcgatcg ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg
    40201 cgtttcgcgc tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt
    40261 tcagaagact tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat
    40321 atagggaaac cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttacccactt
    40381 gatttggaaa aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct
    40441 ttttgtgcat caggaagctc gatagaggat aaaatacgta cgatctacga ggctaagatg
    40501 gaggtaaatg aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat
    40561 aagatgatac ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa
    40621 gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacac
    40681 cctgaaggac tttatttgta tgaagtgaaa tatgaataag aggttatgat agaagtggtc
    40741 agcttcaaga aataagaagg tatttacgaa aattacttct caaaattttg caaaatttcg
    40801 gcttattttc gaagaagcta cttctggaac accgtttaag aaagaggtcg tgagcctgtc
    40861 atttaactct gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt tgatgaatat
    40921 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    40981 aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt gcggacagtc tgaacaggtt
    41041 ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg ttcttctatt
    41101 gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac ataaagaata
    41161 aacaatgcaa tacataggac aaaaaggatt gcacctatcg taaatccttc aggcaagtaa
    41221 ctgaaagtga cagtatgttt acctgctgga acttttatca tcaagaatgc gtctttgaaa
    41281 ttgctgacct tgactggttt cccatcaatt ttagctttcc atcctttgtc ataaggaata
    41341 gttgtcacca acatggaatc ttcagcggta tttacagtcc cagaagcttt tctgccagtg
    41401 gcttccattt cgacaccttt ttctttgatc tgattcattg cggcttgata ggctacaaca
    41461 tctagaccaa cgactttagg ctccatgaaa cttatttctt tcgtcccata aaaacttgca
    41521 gtaaatgtca ctgttgtctc ttgaggataa tagccaagat catagtattg accagaaatg
    41581 ttgatttgcg tttttcgcga tgtaccattg acagtgatcg tcgcactaga actttctaat
    41641 tgcgcaaaat tggttgggaa caaacttaag taagcttgtg tatttgcagg cacatggact
    41701 tcccaagtga gctcttttgg ctggttatct gctgtttctg agtaagtgac gccagtcgtt
    41761 gtctttgtga tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa tgtaaaatac
    41821 tgttcattta gaccagacaa tgtattgatc aagtttgtct gactggtcaa attatctgtc
    41881 actggctgct caacatcatt aacggacaac ggtgcagtaa atgctagtgg caatgcattg
    41941 acattctgat acagtgtata gtcgccgctt tgaccagctt ggcggaagcc atatttactg
    42001 aacgtgcttt cgtttttaga aatgttatat ttgattcctg taaacccatc catcagcaaa
    42061 gtattattgt tataacggat attcaaattt gtccctcttg aacggaaacc taatttatcc
    42121 aggtaagagg aagaattacg atttcttata gaagaaaaca aactgatacc actgtaacca
    42181 tagttgatcg aatcgtttgc agatactgga tccagatttt ccaagcggta aaacgagtcg
    42241 gaatcttttt ttgtttcatt tacaagagct ttcaagtcag gataaggatc agtatataaa
    42301 ctgcgggaag cataattcca atcatcaaga atgccatgga tcatactgtt cgtattgacc
    42361 agtgcttcag cagacatgat caataataac aatatactca gatgattgaa tgaaagtttt
    42421 ttatgctgat aaaatcccac agcaaaagca taaatcccta agaaaaccac tgtcaataca
    42481 aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca tgccaccgca
    42541 aagagagcag ctacgaccaa aatgattcct aacaacagcc caccatcgtc ttgttctatc
    42601 ttttcccatc cttttcctgc aagcaggagc actaagaaag agaacagata ggcataacgg
    42661 aataaaaaca tattcggtgc atgcatacca tgccagaaca aattcaatgg aacaagataa
    42721 aaacttgcga taagtatggc gaaaagactg ccgtacaaaa atttattttt caacggtact
    42781 ttccgactgg caaaataaaa cagacagagg actaatggta gcagacctgc gtaaacgaac
    42841 ggaatcgatc cgtattttgt cgtgtcataa acaccaatca tatttttcat gattagatcc
    42901 agataagaag tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc gccatttgac
    42961 cgaagatcca gcactgctgg caatacaatg atcattgaag cgccgcctgc cagtaaagaa
    43021 gtgataccat aaggaacaat cgttttttta tattctgacc agtttgtcaa tagacgggcg
    43081 ataaaatata agaaagaaaa tacgccaatc ataaaaccca tataaaaact ggataaaaac
    43141 aagagtaaat aactgataaa taaaagtttc ggccgtttaa gatccataac acgatgaatc
    43201 cccaaaatca ctaatggaag ataaacaaat gcatccagcc acatgatgat ctccgaatgt
    43261 gcagtgataa atgagatcaa tgcgtaactg acgctaagtg caacatgccc ccagcgagga
    43321 atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac cttaagcaaa
    43381 gtcagaacat acagtgcatc tggcatcatc tggtttggaa aaaagaacac cagtggcgtg
    43441 aagatcccgc ctagatagta agaaattaaa gataaataat tcaatcctag agaagcattc
    43501 catgtataaa aaatactttg ctttccaaga agaacatttc ggaagctggc atgaaaatta
    43561 gaaaactgtg aaaaggcatc acttgccaaa atacttctgc tgcttccagg atagattccg
    43621 atacttagat aaacaagtgc taaaatcaaa aacggaatca ggaaactagc tgctgcgtag
    43681 cgtccgtttt ctctaaagaa tgactttaaa ttttttttca tatttatttt ccaccaaatt
    43741 tctatgaag
//
LOCUS       DASJPC010000014        43367 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJPC010000014 DASJPC010000000
VERSION     DASJPC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.014"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..2389)
                     /locus_tag="U4A13_000740"
     CDS             complement(8..2389)
                     /locus_tag="U4A13_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1779061.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2379..3026)
                     /gene="recU"
                     /locus_tag="U4A13_000741"
     CDS             complement(2379..3026)
                     /gene="recU"
                     /locus_tag="U4A13_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN1779062.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3090..3629
                     /locus_tag="U4A13_000742"
     CDS             3090..3629
                     /locus_tag="U4A13_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN1779063.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3719..4138
                     /gene="gpsB"
                     /locus_tag="U4A13_000743"
     CDS             3719..4138
                     /gene="gpsB"
                     /locus_tag="U4A13_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN1779064.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4160..4527
                     /gene="rnpB"
                     /locus_tag="U4A13_000744"
     ncRNA           4160..4527
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4A13_000744"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4736..5902
                     /locus_tag="U4A13_000745"
     CDS             4736..5902
                     /locus_tag="U4A13_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN1779065.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            5948..7444
                     /locus_tag="U4A13_000746"
     CDS             5948..7444
                     /locus_tag="U4A13_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN1779066.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7525..8208
                     /locus_tag="U4A13_000747"
     CDS             7525..8208
                     /locus_tag="U4A13_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1779067.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8853..10043
                     /locus_tag="U4A13_000748"
     CDS             8853..10043
                     /locus_tag="U4A13_000748"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN1779068.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10145..11098
                     /locus_tag="U4A13_000749"
     CDS             10145..11098
                     /locus_tag="U4A13_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN1779069.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11167..12516
                     /locus_tag="U4A13_000750"
     CDS             11167..12516
                     /locus_tag="U4A13_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1779070.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12699..13022)
                     /locus_tag="U4A13_000751"
     CDS             complement(12699..13022)
                     /locus_tag="U4A13_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779071.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13151..14314
                     /locus_tag="U4A13_000752"
     CDS             13151..14314
                     /locus_tag="U4A13_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1779072.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14384..15733
                     /locus_tag="U4A13_000753"
     CDS             14384..15733
                     /locus_tag="U4A13_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN1779073.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15778..16209)
                     /locus_tag="U4A13_000754"
     CDS             complement(15778..16209)
                     /locus_tag="U4A13_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1779074.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16597..19239
                     /gene="alaS"
                     /locus_tag="U4A13_000755"
     CDS             16597..19239
                     /gene="alaS"
                     /locus_tag="U4A13_000755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN1779075.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19439..20035
                     /locus_tag="U4A13_000756"
     CDS             19439..20035
                     /locus_tag="U4A13_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1779076.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20157..21305
                     /locus_tag="U4A13_000757"
     CDS             20157..21305
                     /locus_tag="U4A13_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1779077.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21343..21756)
                     /locus_tag="U4A13_000758"
     CDS             complement(21343..21756)
                     /locus_tag="U4A13_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN1779078.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            21835..22266
                     /locus_tag="U4A13_000759"
     CDS             21835..22266
                     /locus_tag="U4A13_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN1779079.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22365..22598)
                     /locus_tag="U4A13_000760"
     CDS             complement(22365..22598)
                     /locus_tag="U4A13_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN1779080.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22781..24451
                     /locus_tag="U4A13_000761"
     CDS             22781..24451
                     /locus_tag="U4A13_000761"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN1779081.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24598..24984
                     /locus_tag="U4A13_000762"
     CDS             24598..24984
                     /locus_tag="U4A13_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779082.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            24971..25669
                     /locus_tag="U4A13_000763"
     CDS             24971..25669
                     /locus_tag="U4A13_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1779083.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25674..26138
                     /locus_tag="U4A13_000764"
     CDS             25674..26138
                     /locus_tag="U4A13_000764"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN1779084.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26135..27046
                     /locus_tag="U4A13_000765"
     CDS             26135..27046
                     /locus_tag="U4A13_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1779085.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27281..27820
                     /gene="pyrR"
                     /locus_tag="U4A13_000766"
     CDS             27281..27820
                     /gene="pyrR"
                     /locus_tag="U4A13_000766"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN1779086.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            27834..29117
                     /locus_tag="U4A13_000767"
     CDS             27834..29117
                     /locus_tag="U4A13_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN1779087.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29130..30056
                     /locus_tag="U4A13_000768"
     CDS             29130..30056
                     /locus_tag="U4A13_000768"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN1779088.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30075..31355
                     /locus_tag="U4A13_000769"
     CDS             30075..31355
                     /locus_tag="U4A13_000769"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN1779089.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31360..32436
                     /gene="carA"
                     /locus_tag="U4A13_000770"
     CDS             31360..32436
                     /gene="carA"
                     /locus_tag="U4A13_000770"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN1779090.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32441..35626
                     /gene="carB"
                     /locus_tag="U4A13_000771"
     CDS             32441..35626
                     /gene="carB"
                     /locus_tag="U4A13_000771"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN1779091.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35680..36456
                     /locus_tag="U4A13_000772"
     CDS             35680..36456
                     /locus_tag="U4A13_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN1779092.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36453..37385
                     /locus_tag="U4A13_000773"
     CDS             36453..37385
                     /locus_tag="U4A13_000773"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN1779093.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37382..38098
                     /gene="pyrF"
                     /locus_tag="U4A13_000774"
     CDS             37382..38098
                     /gene="pyrF"
                     /locus_tag="U4A13_000774"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEN1779094.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38105..38794
                     /locus_tag="U4A13_000775"
     CDS             38105..38794
                     /locus_tag="U4A13_000775"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEN1779095.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38767..39036
                     /locus_tag="U4A13_000776"
     CDS             38767..39036
                     /locus_tag="U4A13_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779096.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39074..39715
                     /locus_tag="U4A13_000777"
     CDS             39074..39715
                     /locus_tag="U4A13_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1779097.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39758..40450
                     /locus_tag="U4A13_000778"
     CDS             39758..40450
                     /locus_tag="U4A13_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN1779098.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40447..41349
                     /locus_tag="U4A13_000779"
     CDS             40447..41349
                     /locus_tag="U4A13_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1779099.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="U4A13_000780"
     CDS             complement(41507..43213)
                     /gene="efbA"
                     /locus_tag="U4A13_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN1779100.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13874 a         7663 c         9440 g        12390 t
ORIGIN      
        1 ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg tcatttgcag
       61 catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat gaagacggtg
      121 tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc tcgcttgttt
      181 ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta gtagagctag
      241 agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag ctggaagatg
      301 gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct ttgtagtaca
      361 gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt gtcacgttag
      421 ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac gattctgaag
      481 tgacaggtgt cattttttta ttgtaacctg tccacacgga aatcgaataa tttggcgtat
      541 agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt gcatactcgt
      601 catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg atttgcgcat
      661 tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata taagctgttt
      721 ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg tcttggaaga
      781 cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa gccgcatagg
      841 ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct tccgtattac
      901 tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt tttaagaaag
      961 aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca ttacgggata
     1021 agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag tttcctacag
     1081 gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca gtagaatatt
     1141 tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa ccaaaatcac
     1201 gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg tgacgtccgc
     1261 caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca acttgcatct
     1321 cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt ttcttttggg
     1381 cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc ttatctgttt
     1441 tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta gtattgtcat
     1501 cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct tgatcatatt
     1561 ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct cgtcttttct
     1621 tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc atcccagcaa
     1681 gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac atctcagaag
     1741 ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag taggtcaaga
     1801 tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct tgggccttgc
     1861 gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc aattgctgtg
     1921 tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca gaaccgatga
     1981 tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc gacacgatcg
     2041 catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt tctgcgccta
     2101 aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta gcgtctaatt
     2161 ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct acacctgcta
     2221 aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat aatccttttt
     2281 tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca ctctttttag
     2341 gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt cttgctttct
     2401 ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg aatccgcgga
     2461 gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg tagtgatttg
     2521 cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt caaataaaaa
     2581 cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg ttcgaggcat
     2641 gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga tgttttgttc
     2701 ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt atagtctgta
     2761 gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt aggatagtct
     2821 actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc gcgggaaaga
     2881 taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt gccgaattca
     2941 gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt ataataaggt
     3001 tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca ttatagcaaa
     3061 aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc tatgtgtctg
     3121 ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc caagttgtta
     3181 aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa tgggtacttg
     3241 tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa ttgaaagaag
     3301 aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt aataactgga
     3361 atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat gtcgaagctg
     3421 ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt tttatacttg
     3481 agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa acgaagtatt
     3541 tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg ctgatcagta
     3601 tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat caactttttt
     3661 cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag gtgtaaatat
     3721 ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa caaaaatgcg
     3781 cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag attatgaagc
     3841 ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg caaaagtagc
     3901 tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag aggcaccaaa
     3961 gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg aacgtgaagt
     4021 atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac ctaatccaaa
     4081 taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc aattttaatt
     4141 tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca ggctgaggaa
     4201 agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa accataagct
     4261 aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat gcttaagtat
     4321 ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga acgcggtaaa
     4381 cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa gaagaacagg
     4441 acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct ccctggaaaa
     4501 gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag gtcgtgacaa
     4561 aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa tacttcagat
     4621 gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat ttgagtaaaa
     4681 gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat tgtgtatgga
     4741 aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag cactggttgg
     4801 taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg cacgttttga
     4861 aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag atcgagtaaa
     4921 aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa acgtaaaagc
     4981 attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg gaaaatcgat
     5041 caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag caatcgttga
     5101 acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa ctggcgcaca
     5161 ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg atacgacagg
     5221 accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat tgaaagaaaa
     5281 catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat tttatgatcc
     5341 tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata atatcgcacc
     5401 aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag aaatcatgga
     5461 aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag atattactgg
     5521 ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag aaatcggatt
     5581 aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg ataaagaata
     5641 cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag aaactgttcg
     5701 gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga gtaaatatat
     5761 cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca aaaaaagaaa
     5821 attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc gtccgcctag
     5881 aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat gtttggagga
     5941 atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc ttctacaaca
     6001 agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag ctagtgcata
     6061 ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa tcggtccaaa
     6121 aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg aagttggaac
     6181 tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac cggaagatct
     6241 tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa aagctagaga
     6301 aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat taacaaaaca
     6361 gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc tgaatcaata
     6421 cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta aagaaggaat
     6481 tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag actttttaaa
     6541 tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg aacaacttgg
     6601 ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga ctggaattaa
     6661 tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa tggctgtttt
     6721 tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg ataaatatgc
     6781 gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat cgttgtttaa
     6841 cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt tctttcaaga
     6901 atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt cattgaacca
     6961 tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc tccatatcat
     7021 cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg ctgaactacc
     7081 tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa atgatgtgga
     7141 aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc cctcttatgc
     7201 attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa tagatgtgga
     7261 aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag agcatatcca
     7321 tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac atgaaccgct
     7381 taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat atcaggtaaa
     7441 ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt tttcagctat
     7501 ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga ttatagtatg
     7561 gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag agttgtcaag
     7621 ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac gcttgcagca
     7681 gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga ggcaaagcaa
     7741 cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc gatgattttt
     7801 tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga actatctctg
     7861 gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat cggcattttg
     7921 cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga actgtatccg
     7981 caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa tcatttttat
     8041 gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga agaagccaaa
     8101 cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg gtctattgac
     8161 gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg gaggttgtga
     8221 cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac ttctggaaca
     8281 ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga acaatttcaa
     8341 aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg gcaggagaac
     8401 accgtttatt cggaagaata ggactgtgac aagacttttg tcacagtcct tttttcgaat
     8461 tcaacaagta aaacttttaa gaaatattca gagagtaaaa ttaaaaattg ctgataaacg
     8521 aagatggaaa ttttatgtat ttttattggt ctatgcaata aataaattaa atattcagaa
     8581 tacaattgat tttaacgaaa gggcttctat caatggttgt gttgtattta tctgcccagg
     8641 tagagtaaaa tagatcatta tctaaaaggt aattgttcat actatataag tcatttgaaa
     8701 gtaaaaagat actgtaaata aaaaatgtgg ttaaataatt ctcataatca gtgtaagcgt
     8761 tatcaaaaat atagcatact tgctttgtag atgaatgaca cgatatatgt ttggtatgtt
     8821 ctgcaagaca gatgaatatg gaggtggaga caatgtcttc caaagaacta acaatgtatt
     8881 tagagaaaga attggatcaa ctgaaagaaa aggggttata caatacgatc gacatactag
     8941 aaagtgaaaa tggtgcttgt atcatcgtag acggaaaaaa gatgatcaat ctagcatcca
     9001 acaattatct tggatttgcc aatcgcgaag aattgaaaaa agcatgtatt gaagcaaccg
     9061 aaacttatgg tgtaggagca ggagcagtcc gaacgatcaa tggttcgctg aaaatccatc
     9121 agcaattaga agaaaaaatc gcagaattca aagggacaga agcagcaatc gcctttcaat
     9181 ctggattcaa ctgcaatatg ggcgctatct ctgcagtaat gacaaaagaa gatgctatct
     9241 tatcggatga attaaaccat gcatctatta ttgacggttg ccgcttatcc ggagcaaaaa
     9301 tcatccgaat taaacatcaa gatatgaaag accttgagaa aaaagcaaag gaagcgatag
     9361 aaagtaagaa atataaaaaa atcatgtata tcacagatgg tgttttctcc atggatggag
     9421 acattgcacg attgccggaa attattccga ttgttgaaaa atatggactg atcacctacg
     9481 tcgatgatgc gcacggctca ggcgtaactg gaaaaggtgc aggaaccgtc aagcattttg
     9541 gattaagcga caaaatcgat atgcaaatgg gcacgttatc gaaagctata ggtgttgttg
     9601 ggggttatgt agctggatcg aaaacactaa tcgactggtt gaaagctaga agtcggccat
     9661 tcctattttc tacttcgcta actccaggtg cagctgcagc tgcattggct tcgattactt
     9721 tgatgcaaga acatccggaa cttgtcgaaa aagtttggga aaatgctaat tattttaaag
     9781 aagaattgaa aaaagtaggc tataacatcg gtatgtctga aacacctatt actccagtca
     9841 ttttgggaga cgaaaaagtg actcagactt tctctaagaa gctgatcgaa catgggattt
     9901 atgctaaacc aatcgtttat ccaactgtgc ctcttgggac aggcaggatc agaaatatgc
     9961 cgactgcgga acatacgagg gaaatgcttg atgaagcagt agcagtttat caaaaaatag
    10021 gaaaagaaat ggaaattatt taactaaatt tagttttttc gtctatttaa aattagagaa
    10081 catgttcacg catatattgt taatcatctg aagatagtag taaaaaagat cagggaggat
    10141 catcatgaaa aaagtattgg tgacaggatg tttaggacaa attggttcag agttgatcac
    10201 acggctacgt caagaactag gcagtgaaaa tgtcattgcg acagatatac gcatgcctga
    10261 agtcattgag aaaaacgata tctttgagat cctagatgta atggattacg aaaaaatgcg
    10321 cagcatagca gaacaatatc aagtggacac attgattcat ttggctgctc ttttgtcagc
    10381 tgttgcagaa gcaaaaccac aattagcctg gaatttgaac gtgactgggc tggtcaatgc
    10441 attggaagtc gctagagaat tccagctgaa attttttacg ccaagttcaa tcggcgcgtt
    10501 tggtccaaat actccaaaag atcatacccc acaagatact cttcagcgtc ctacaaccat
    10561 gtatggggta acgaaggtag ctggagaact actatgtgat tattattatg tgaaatatgg
    10621 cgtagatact cgtggtgtcc gtttccctgg attgatttct tatgcaacct tgccaggagg
    10681 cggtacgaca gactacgcag tcgatattta ttattcagca atcaaagaag gaaagtacac
    10741 tagtttcatt gatcaaggta catcaatgga tatgatgtat atgccagatg cgatcgatgc
    10801 cattataaaa ctcatgaatg cagatcctga tcgattggtt catcgaaatg ccttcaatat
    10861 atcggcgatg tcttttgacc cggaagagat caaagcatct attcaaaaaa tcataccaga
    10921 ttttgaaatg gattatgatg tggatcctgt acgtcagtca atagcagaat cttggccaaa
    10981 ttcactagat gtttcttgtg cgcaagagga atggggcttt tcgccgaaat acgacttaga
    11041 tgtgatgaca aaggatatgt tggaaaaact gaaagaaaaa ttggtagaga cggtataaaa
    11101 caagaaccac atccaaaaag ttttttatac ttttgggatt ggctatggta tgaaagggga
    11161 caagggatgg aagaatatca agccacgcca gtaaaagaag taaaaataga aaatgaatta
    11221 aaacgtacga tgggattttt tactgcttta tcgactgtta tgggcacagt gatcggtgca
    11281 ggggtatttt ttaaagccgc aagcgtagca gaagttacag gatccgccag tttgcatatg
    11341 ttttcttggt ttctcggtgg tatgatttct gtctgtgcag gactaacagg agctgaactg
    11401 gcagctgcta ttccagaaac aggagggatg attaaatata tcgaacggat ttacggaaat
    11461 acagcagctt ttttattggg ctgggcgcaa gtcgtgattt attttccagc aaatgtagca
    11521 gccttgtcga tcattttcgg tacacagttc gtcaatctat ttggtctttc ccagtcaatg
    11581 attgttcccg tagctgttac agctgctgta tcgatcctgt tgatcaattt tttaggttct
    11641 aaagctggag gggcttttca gtcgattacg cttgtctgca agcttattcc tttgtttgtg
    11701 atcgtcattt tcggtttgtt ccgacaagaa ggagtagact tccaactgtt tccgatccaa
    11761 gcaggagaaa atctttcatt tttctcagcc ttgggagcgg gcttgctagc aacgatgttc
    11821 gcatacgacg gttggataca cgtaggaaat atttcgggag aattgaaaaa gccggcgaaa
    11881 gatttgccaa aagcaatttc tttaggaatc atcggaatca tgatcgttta tttattagta
    11941 aatgcggtgt tcttaagaac cgcatctatc gacggagtcg caggaaacag caatgcggca
    12001 agtgacgttg caaaaatgat ttttggcggc ttcggtggaa gactggtcac agttggtatc
    12061 ttgatttctg tttatggaac catcaatggc tataccctta ctggaatgcg tctcccttat
    12121 gttatggcaa aagaaaacaa cctgcctttt agcaagctct ttgctaaact tcatgataaa
    12181 actaaagttc ctgtagccgc aggtatctta gagctagtca tcgctattgg catgatgatg
    12241 atcggtggtt ttgatacact aacggatatg ctgatctttg tcatttggat tttttatacg
    12301 atggtctttg ttggtgtcat tcttcttcgt aaaaaagaac ctgatttgtt tcgaccatac
    12361 aaagttccaa tgtatccgtt tattccacta gtagcaatca ttggagggac ttttatcgta
    12421 tcaagcacgc ttatcactca gaccttttta gcttcgatgg ggatagtatt gacacttgct
    12481 ggtatcccga tttatctcta tttaaaacgt agatagctaa aacattcgaa ttttattaga
    12541 actgcacttc tctgccaaac aaaatatttt atttaaatga ggagtacgtg gaagaaatgt
    12601 caaaagtact cgttaatttt gatcacgtaa atgtcgtttt gtctacgatg aaatagcaaa
    12661 gtaaaagaca gaaatcgggc tcaaccgatt tctgtctttt actttgattt gcgtaactga
    12721 actattcggc gtacgccgcg ataaatattt aataaaacca agcaaccaga aatgaaaatg
    12781 ggaagccaaa tcagatagac agggtagcca agcactttag acaaagcatg tggagtcatt
    12841 ttatccattt caagaagtgc ttgctgccaa gtctgaagat tgaagactag catgaaaccc
    12901 gaaataatca ggagaataaa tcctaaataa tgaattgtag ttctttttct tacataagcg
    12961 atcgtagcat aaaggaacag taaaaaaata ggtacgccaa aaacaaagta catattcagc
    13021 atctcgacac cccctttgtg tttatcttac tttactttga aagaaatgag aaataatagc
    13081 ctcgttactt gcttgaaaca gcaaaggact gctaagataa gagagataca aagaatcaag
    13141 gaggagtcat atggcaagtt atatcgaacc gattcgtttt gcgctgattg tttttccatt
    13201 tttagcctta gcgatctcat ctgttttttt tatttatgaa tatcgaaagt atggaacatt
    13261 tctagtaaca agggcagtta ttttgtattc tttcgttttt tatttattgt gtgcgtattt
    13321 tttggtgatc ttaccacttc cggcaaagga gacggttgcc caaatgacag ggccaaagat
    13381 ggagctgcat ttatttgctt catggcaaca tttcagagac aacacggtat tggtcttgtc
    13441 tgaacctagt acctacctgc cggcaatgaa gcagagtgta tttttggaac cagcttttaa
    13501 tgttttatta ctcataccat ttggcattta tttaagatat tatttccggc tttcttttat
    13561 gaaaacgatg attgctagtt tctgcctttc tctatttttt gaattgacgc agctaagtgg
    13621 actttatttt atctacccaa gaccataccg cttatttgat gtgaatgatt tagtgacaaa
    13681 tacattgggt ggaatcattg gttttgtcgt tacgccgtta tttacattta tgcttcctac
    13741 aagaagacga atggatgaac tttcttatga aaaaggaaag actgtctcat tgactcgacg
    13801 cttggtcgct tatttgatcg actggacatt tttatctctt attaatttgg tagcggcgat
    13861 tttctttaaa attgcgttcg attcaggggc agttacttct catatctggt ggcttttatt
    13921 agaagtaggg gtatacttta tgattgtacc gtttataacc aatggatgga cacttggcaa
    13981 aaaaattgtg cgtatccgtt tggtggaaga agaccgcgaa cgaatttctt taaaagcatt
    14041 gatgatccgc tacgggtatc tgtactttct tttttatggt ttaagctggt tggggcagtt
    14101 gcttcagcaa atcatgaatt cgacaacgaa tacggtgttg cagatgagtg cagtattcat
    14161 ttctctcggt gtcttgttac tacaaggtat ttttatttta aatatcttgc tatcattttt
    14221 gagaaaaaaa cggattctgt tttatgaaaa agctagtcac acatatactg ttagcacgat
    14281 tggccaaaca aaggatgatg attctcgaaa ttagctgagc aaaaacgctt tgtctgtgtt
    14341 ataatgccaa agtcgtgata atttaaagag aagaggtgca cgaatgaata attttgatat
    14401 attccgcttc aaaccgttta tcaatgaagc gttaaccgca aagggcttca aacagcccac
    14461 agaggtccag gaacgtttga ttcctttaat tgcaaaagga aaaaatatca ttgggcaatc
    14521 tcaaactggt acaggaaaaa cacatacgtt tttgttgcct ttgtttgaaa aaatcaatgc
    14581 agaaaaagag gaagtacaag ttgttattac agctccaagt agggaactag ctgcacagat
    14641 ctatgaagca gctacacaaa tcgctaaatt ttctacaccg gagatccgtg tctcgaattt
    14701 tgtcggcggt acagacaaac agcgtcaatt aaatcgtctg catcaccaac agccacaggt
    14761 tgtagtaggc acgcctggta gaatattaga catgatgaat gaacaagcgc tgaagattca
    14821 caccgcattt gcatttgttg tcgatgaagc ggatatgaca ttggatatgg gttttttaaa
    14881 tgaagtagac caaattgcaa gtcgattacc agaaaagctg cagtttttag ttttttctgc
    14941 cactattcca gaaaaacttc gtccattctt acgaaaatac atggaaaatc caatcattga
    15001 agaaatcaaa ccaaaagcag ttatttctga agacattgaa aattggctga tttcaacaaa
    15061 aggtaaagat gagaatcaaa tgatttatca actgctgaca attggtcatc cttatttagc
    15121 aatcgtattt gccaatacaa aacagcatgt ggatgaaatt gccgattact tgaaaaacca
    15181 aggcttgaaa gtagcaaaga ttcatggaga catcacccca agagagagaa aacgagtgat
    15241 gcgacaagta cagaatctgg agtatcaata tgttgtagcg actgatttag ctgctagagg
    15301 gatcgatatc gaaggtgtct ctcatgttat taatgcagaa attccagaag atctagactt
    15361 ctttatccat cgtgtcggac gtactggacg taatggctta aaaggaacgg ccattacgtt
    15421 atattcgcca aatgatgagc aagccattga tgcgatagaa aaattgggcg tctcatttga
    15481 accaaaagaa atcaaaaacg gggaaatcgt caaaacatac gatcgtaata gacgtgccaa
    15541 acgagaaaaa tcaaaagatg tattagatcc cacgctgatt ggtcttgtga agaagaaaaa
    15601 gaaaaagatc aagcctggtt ataagaaaaa aatcgattgg gcaattgcag aaaaaaataa
    15661 aaaagaacgc aaaatcgaac gcagacagca aagccgtact gctcgtaaaa ataagaaaaa
    15721 ttcacataaa taaaaaacaa gaggttgtga caaaaggcgt gtcacaatct cttgttttta
    15781 agctagactt tttttcattc cgatacaaag tacttcatct tcaataaaag gttctgtgca
    15841 agtatgatac ccttgttttt cataaaaatc tacagccgtc atttcagcta ctacataact
    15901 agtctgacaa ccttctgaaa ccgcccgttt ttcagcagct tcaagcaact gaccgcctaa
    15961 cccttttcct cggtattctt ttgctacgca aaatcgttcc ggcttgaagc aagtaggtgt
    16021 tttcttttca taacgcaaag tagctactgg tcttttttga tcaagaaata gataataggt
    16081 ttgttgcggg atatcttttc cgtcgaattc tagttctgca ggaatccctt gttctaatac
    16141 aaaaacttga tatctcaaaa agcagctagc tgcgcgtatc cattcatccg ctccaactat
    16201 ttttttcatc atattcctcc ttgtcatgaa tagcagagat gtattgattc ctttattctt
    16261 atcgtaccaa aaatcgtgga gtatgaccaa aaggaaatga aaataaaaat tgagatattg
    16321 acagtgatca atagaatgcg tatactaatt aagtacgaaa aggatatgat ttgtttttta
    16381 agactcacag aaaattcttt cttggctgaa aaaagaatag tcactaatca aattgctccc
    16441 tttttctatt ttggtagaaa taccgacgca gctcagcgtt atcgagttta agaggtaatg
    16501 attacacatc attgcaatca aggtggtacc gcgagttttc gtccttggct gtgatggtgt
    16561 ttttttgtat ttaatttata gaaagcaggt aatgacatga aacaattaag tagtgcacaa
    16621 gttcgacaaa tgtttcttga tttcttccaa tctaaaggac atacgattga acctagtgcg
    16681 tctttgatcc ccgtgaacga tccaacctta ttgtggatca actctggtgt tgcaacattg
    16741 aaaaaatatt ttgatggttc agttgtgcca gaaaatccaa gaatcacaaa tgcccaaaaa
    16801 tctattcgaa caaatgatat cgaaaatgtc ggtaaaacag cacggcacca tacgatgttt
    16861 gagatgctgg gtaatttttc gattggtgac tatttcaaaa atgaagccat ccactgggca
    16921 tgggaatttt tgactgatcc aaaatggatg gcattcgatc cagaaaaact ttatgtaaca
    16981 gtttatccaa aagatacaga agcaaaacgc atctggcatg aagaagttgg cctgccacta
    17041 gatcacatta ttgatgtaga agacaacttc tgggatatcg gtgcaggccc atgtggtccg
    17101 gatacggaaa ttttctatga ccgtggagaa gcttataacg atttagcaga agacgatcct
    17161 gaaaactatc caggaggaga aaatgaacgt tatttagaaa tctggaactt agtattttca
    17221 gaattcaacc ataagcctga taacacattt gaaccacttc ctcataaaaa cattgatacg
    17281 gggatgggat tggaacgcat ggtctcaatc gttcaagatg cgtcaacgaa ctttgagaca
    17341 gacttgttta tgcctattat ccacgccgta gaaaagctaa gcaaacaagt aaaatatggt
    17401 gaaaacccag taacagatat ttcttacaaa gtaatcgcag atcatattcg cgccctttca
    17461 tttgctattg gcgacggtgc attgccatcg aatgaaggga gagggtatgt cttacgccgt
    17521 ttgcttcgtc gtgctgtcat gcatgggaaa aaattaggta ttgaacaagc attcttatat
    17581 cgtttagtcc ctgttgttgg ggaaatcatg gtaagttatt atcctgaagt acttcaacaa
    17641 aaagaattta ttgaaaaagt tatccgtaca gaagaagaac gtttcaatga aacaatcaat
    17701 gaaggtttgg aaatcttaaa tcaagtgatc caagaagtaa aaaacgaaaa cgggacgact
    17761 ctagcaggaa aagacatctt caaactatac gacacatatg gcttcccggt agaattaacg
    17821 gaagaggtgg cagaagatga aggactgaaa gttgatcatg ctggttttga aaaagaaatg
    17881 gatgcacaac gcgaacgtgc tcgctctgcc cgcagtactg aaaaatcaat gggggtacaa
    17941 tctgctttat tgacagatat caaagtagaa agtaaattca ttggatacac agaacaagaa
    18001 tcagatagtc aattattggt catcgttaaa ggtgagtctc ttttgaatga agtcgcagaa
    18061 ggtgaagctc aaatgatctt tgatcaaaca cctttctatg cagaaatggg tgggcaagta
    18121 gcggatcaag gatggatcat gaatgaagta ggcgaaatcg tagcagttgt tacagatgtt
    18181 caaaaagcgc ctaatggtca atttatgcac accgtagaag taaaagcacc aatgagtgaa
    18241 ggacaaacct atcatctatt gatcgatcaa tcgatgcgca atcggatcat caaaaaccat
    18301 acagcaaccc atctactgca caaggcattg aaagaagtac ttggcgagca tgcaaatcaa
    18361 gctggttctt tagtcgctcc tggacattta cgattcgact ttacccactt tggtcaaatc
    18421 acttcagatg aattagctga aatggaaaga atcgtcaacg aaaaaatctg ggaagctatc
    18481 ccagttgaaa caattgaaac agatatcgat acagccaaaa acatgggtgc aatggctttg
    18541 tttggtgaaa aatacggaca tgacgttcga gtagtaaata ttggcggctg gtcaatcgaa
    18601 ctgtgcggag gcacacatgt agcaaataca gaaaacatcg gaatcttcaa aatcgtttct
    18661 gaatcaggaa ttggtgcagg cgtgcgacga atcgaggctg taacaagtaa agaagcgtac
    18721 gaactacttg aagaagaaga aaaacaacta aaacaagtag caacaattgt taaatcacct
    18781 caattgaaag aagttgtttc aaaagcggaa caattgcaac agcaagtaag agagctacaa
    18841 aaggaaaatg aacaattagc tagcaagtta gcgaatcaac aagctggtga tgtattcaaa
    18901 gatccagaaa cgatcaatgg aaaaacaatc attgcagcac aagtaaaagt gaaagatatg
    18961 aatcagctgc gtcaattagc tgaccaatgg aaacaaaaaa atatttcaga tattcttgtt
    19021 ttagcaaacg aacaagaagg aaaagtcagt ctgctagcgg caatgacgaa agaatcaaat
    19081 gaagctggac taaaagctgg cgacttgatt aaagcaatcg caccgaaagt aggcggtggc
    19141 ggtggtggac gtccagacat ggcacaagct ggcgggaaaa atcctgctgg tattccagat
    19201 gcacttaaag ctgcgaaaga atgggttgcg caacaatagg gcgtttacgt ttgacagaaa
    19261 aacaaaagtt caaacattga acgaaacatg attatggggg cgaagacaaa caagtcttgc
    19321 cctcatactt ttttcaattg gatggcgcat ctaatcaaat ggtcggattc ggatggtctt
    19381 tagttgacga aagtgacaga aatttttacg attagtataa ataaaaaggg gtgaaaaaat
    19441 gaatagtaga aataagacct ttcgactagt cttacgtgcg atcttgttag caatcatcat
    19501 cgtacaagcg atggttcctt ggctggggtt tattccatta ggattcatta gtttaacaat
    19561 tatccatatc acagtaatta ttgctgcagt agtattaggc ccaaaagatg gcatggtgat
    19621 tggcttgttt tggggaatcg ctacaattgt tcgtgcctat gccatgccaa caacgccatt
    19681 tgatacatta gtgttcacga atccgattat ctcggtagtt cccagagtac ttgttggctt
    19741 agtagcaggc ttggttttcc actggattta tcaaaaaaat aaatcaataa cgatcagctc
    19801 agcctttgca ggggtactgg gttcattggt caatacagtc ttagtgttag gattcatggg
    19861 attattttat acaggtgcaa cggcagatgc ctatggtgta gactctgctc ttttatttaa
    19921 aaccttagca ggggttgcag caatcaatgg gataccagaa gcaataggtg cgggaatcat
    19981 aacaccatta ctagcaaaag cgttatttgc agcaacaccg ttgaaaccag aataatgacc
    20041 atcttttaat agtaatagga cgcacgatag atagaaaaag gaagagagga gaggaaaaca
    20101 atcgtcctct ccatcttttt acctgagcaa caagcaaact agaaggggta aaaaacttgg
    20161 ctaagaaaca agaagaaaaa aagaaacaaa taccgcaaca gcggacaaaa aaaggagtac
    20221 tagcgggtat cttaggattg gttacgaatc tgatcttgtt tgtctctaag ctaatgatcg
    20281 ggcttttttc aggaagtgtc tccattatgg ctgatgcagt gaacagtcta tcagatactg
    20341 cttcatccat attgactttg atcggattta aaattgctgc aaagccagca gatcaggaac
    20401 atccttatgg acacgaacgc ttcgaatata tcagtgggtt atttgtttcg atcatcatta
    20461 cttatgtagg atttcaattt ttagagtctt ctgctgaaaa aatcatccat ccagaaaatg
    20521 tctctttaac acccatcgtc ttttttgtcc taatcatttc catctttttg aaatttgcac
    20581 aaggcaaaat gtatcaggtt attgcgcaat ccattgattc tgaaacattg agagctacag
    20641 caacagatag ttataatgat gtctttacta cactggctgt attgctttca gcagcaatcg
    20701 aatgggtaac tggatggcgt attgatggat atataggttt tgctttagca ttatatatcc
    20761 tttatagtgg attgacgatg cttcgaaatt ttgtatacga actattagga agtcgcccaa
    20821 cgccagcaga gattgaagaa atggaagagc atttaaatgc ttgcgatgat atacttggct
    20881 atcatgattt attggtgcat aactatggac ccaataaacg ctttgcatca gtccacatcg
    20941 aagtagatga atcaatgaat ttgaatgtgg ctcataagat tattgatctg atagaaaaag
    21001 actttaaaaa gtctttggat gtggaacttg tctgtcactt ggatccaatg cctgttgata
    21061 acgaaaaata ctataatatc ttgcggcagt taaagcaaat tcttcttgaa acaggtacag
    21121 ggcttaatat tcatgatttt agagtaattc atcaaggaaa aacgctgcaa tttgatgtag
    21181 tggtccctga gaaattccag tttgttgata cagaactaga aagaatctta cgtagtcaaa
    21241 tagaagaaaa aatcggagat taccaattgg aaattacgtt tgatcacaat tatcttttat
    21301 attaaatgag aaactataaa aagactcctt aaagttttta atttaaagag tctttttttc
    21361 ttttaattgt ttatggagag cctgacgtgc aagattatct gctcctttat ttttgttttc
    21421 gggcaaccat tgcaggatca ataaagaaaa ttcggataga agttcttgga ttctttgaag
    21481 gtagggaagg aagagctcgt tcttcgttgc gtttttgtcg accgtagaaa ctagtgtttt
    21541 gctgtctgaa aaacagaaaa tcgtttcatt ctgtaagtcg tttttcttta gaaattctaa
    21601 agtttttaaa aagacttcga actctgcttg atggtttgtc cctatagaaa gcgggataga
    21661 tagctgttct tgtttacctt gatagacaat caagattcca cctccactag gaccagggtt
    21721 gccttttgta gaggcatcaa tgtatacttt taacataaaa agagattctc ctttttgttc
    21781 aaactaatgt tatggtaaga ttatatcatt actgaatcgg tgagaggtta gagaatgaaa
    21841 aaaaatattc gatggcagcc tgaactggca catacgatca tctattggtc attgaccttg
    21901 atcattctgt tttttagtct gattctttct ttagaaaaca caggtcctta ttggaaaagc
    21961 aattttgttt taggtatttt tcttgttttt ctatttctag gtttgcggcg ttcttttatt
    22021 ttaaaatcaa actctgtgat tatacgttat gctgcttttt ggaaagaccg agaaatatct
    22081 gtcggacaga ttcgatcttt gacgccaact aatactgggg tgcaaatgat gttgcaagat
    22141 gagaaagccc catctgtttt catgatgcga aaaaaagatc aagtagtcct ttttgatcat
    22201 tttgaaaaaa acggacccaa atcagaatca attccttatc cttatacgaa aaaaagaaaa
    22261 agctaatttt aaaaatcaga acctaaaaaa acgaattttt acaggaaaag aaaagagtga
    22321 gacgtacggt tcatcccgtt tagcgtctca ctctatttgt ttatctattc atgcatacta
    22381 gtatcggtag tctgaacgtt gcctttcttg acatgggcag cttgtgttcc tcgattccct
    22441 tcgatgatct caaattcgac cacttgattt tcttccaaag ttttgaatcc ctcttcttca
    22501 atcgcagtaa aatgtacaaa aatttcttct gtgtcatcat aactgataaa gccgtatcct
    22561 tttttgttgt caaaccattt tacaattcct gtggtcaaag cactcacgac ctttctaaag
    22621 tctgatcata gccttcatta taaagagtat ttataagtac gtcaaataaa ttaaaaacct
    22681 gtaattttat gttttttttc tgaataaaaa aaggtataat tggcatatct taacaaagaa
    22741 gatattttgt tgaataaagt tcgtgaaaag gggtaatcaa atgaagaatg atctgcaaat
    22801 ttcacaagaa acagagctag aaccgattgt tgaagtggct agtaaattag atcttaagga
    22861 agacgatttg gaactgtatg ggaaatataa ggcgaaaatc aatttttctg gaatcaatcg
    22921 atcaaaggat aacgctgagg ggcatttgat cttagttact tccatcaatc caacaccagc
    22981 tggcgaagga aagtcaacga tcactgtagg gttaggagac gcgctcaata aaatcaataa
    23041 aaaatctgtt attgcattgc gagaaccctc acttggtcct gtgatgggaa tcaaaggtgg
    23101 tgctgcaggc ggaggatatg cacaggtatt gcctatggaa gatatcaact tgcattttac
    23161 tggtgacatg catgctatca caacagctaa taatgcatta tctgctcttt tagataatca
    23221 tattcatcaa ggaaatgaat tgaacatcga tgcccgccga gtgatttgga aacgggtagt
    23281 cgatctcaat gatcgtgaac tgcgtaaagt aattgttggc ttgggtgggc caatccaagg
    23341 tgttccaaga gaagatgggt ttgatatcac agttgccagc gaaatcatgg ctatcttgtg
    23401 tcttgcggca gaccttgaag atttaaaggc tcgtcttgca aggatcgttg tcggctatac
    23461 atttgaccgt cagcctgtga ctgctggaga cttgaaagca gaaggcgctc tggcattact
    23521 gctgaaagat gcaatcaagc cgaatcttgt acaaacaatt tacggaacac cagcttttgt
    23581 ccacggggga ccgtttgcca atatcgctca tggctgcaat agtattttag ctaccaagac
    23641 agcattgaga ttagcagatt acgttgtcac agaggcagga tttggtgcag atcttggcgg
    23701 agagaaattt ttagatatca aagtacctaa cttgaaaaaa gcgccggatg ctgtggtgat
    23761 tgtcgccact gtccgtgcat tgaaaatgca tggcggaatg aaaaaagatg aacttaaaaa
    23821 tgagaatctt gatgctttga aagtaggttt tgcaaattta aagcgccata tacgtaatat
    23881 ggaacaatat cagctacctg tgatcgtcgc aatcaatgaa ttcgtaacag atacagacaa
    23941 tgaactcaca ctgttggagc acttgtgtga aaatcagggg attcttgcca agagagcgag
    24001 tgtatgggca gatggagcag aaggcggcgt tgatttagca aaagctgtag tacgattgat
    24061 tgatagaaaa gaagctaatt acaagccatt ataccgccta gaagaaacga tacaagaaaa
    24121 gacagaaaca atcgtcaaga aaatttacgg aggaaatggt gttgttttca gcaaaaaagc
    24181 gcaaaaacaa atagaagagt ttacgaagaa tggatgggac aaactgccga tttgtatggc
    24241 aaaaacacag tattcatttt ctgatgatcc ttcactgcta ggtgcaccgg aagatttcac
    24301 catcacgatc cgcgaaatca tcccaaaatt aggggcagga tttttagtag ccttgactgg
    24361 agatgttatg acgatgcctg gtttgcctaa aaaacctgct gcattgaata tggacgtgac
    24421 aaatgacgga gaagtgttgg gattgtttta agcttgatta taatctacca tcgaaggagc
    24481 tgtattttga ctacagcttc tttttttaga cgatagggaa ttaattttgt ttacactagg
    24541 atcattcttt ttctttattg tattttgagc tatactaaag cataatagga gggtatgatg
    24601 aaaacgacat ttataagaaa aaccggtttt tacggtatgg gtagctcgat tcagctaaga
    24661 atcaacaatg aaaaacgcag tgttctcagt catgaacaaa agataacgct tgatctggaa
    24721 gtgcccttta cgatgcaggt caccttttat tggctaaaaa gccccgttta tacagtgaaa
    24781 gaagcaaagc ctgcttatgt gatcacaatg aattacttga ttcttcaaat ctatccgttt
    24841 ttatttctag caactgggtt gggggccgtt ttcctacaaa atttattttt cagttttttt
    24901 gcagttattg tgttgattgc gttctttttc tatataaaaa accgggcata tttgatcaag
    24961 gaggaaaatt atggcaaact ctgatctttt tttaatcagt ttcaatcgaa tcgaaaaatg
    25021 gatgagagac gagatgggaa atgcacgaaa tatgggattt accgaacttg tcagacgcct
    25081 agctcaaaga aaacaattaa tgatcagaaa atacgaagat gatcttttac agcttgctca
    25141 gttaaggaat gcgatcgtcc atgatcggat cgcagtcgat tttgtcattg cagagccaaa
    25201 tgattggaca gtcaagcgaa tccagctgat cgaacaagaa ctgattcagc ctgaaaccgt
    25261 gctgcctcga tttgcaaaac gtgtaacagg ttttgaacaa gatcttccgt tacttgaact
    25321 gttaaagata gtagcagaaa aaaggtattc acaatttccg ttatacaata aaggaaaatt
    25381 tgtagcattg attactttac gtaatatcgg gttttggcta gcaaaagaaa gccaaaaagg
    25441 tccggttgat ttaacaaata aacgagcgtt ggatttgatc atccaaaacg gaaagtacac
    25501 aaattatcat tttgttccag ctaaaacaca tatttacgag gttgaagcga tgtttcgtga
    25561 gcaagggact ttggaagccg tacttatcac taaagatggg aatcccgatg gtaatctgct
    25621 cgggatcgta cgacctcgtg atatttataa acaaatagaa aaggattgat tttttgttaa
    25681 ttatttattg gatattcagc gctttgctag taggagtgga tcagttggtc aaatggtgga
    25741 tcactgataa tctagcgctt ggagagacga aaaatcttat accaggtatt ttgtcactga
    25801 atcatattcg aaacactgga gccgcatgga gtatgctaga agggaagatg tggttcttta
    25861 ctatagtgac actcattgca gtcgttgtga ttctcacatt gatgattaaa aatcgtgaaa
    25921 aaggtaatcg ctggttcatg attggattga gtttgattct agctggtgcg attggcaatt
    25981 ttatcgaccg tattcgtctt ggctatgtag tagatatgtt ccagacagat tttatgagct
    26041 ttccgatttt taacgtagca gatgtgacac ttgtatgtgg agtgatttgt atgttaattt
    26101 atattatttt agatgaaaag gatcaaagaa aaaaatgaca gaacttaaaa cgacgattca
    26161 ccatgaatct ggtaggatcg ataaagtatt gactaattta ttcaccgatt acagtcgttc
    26221 gcaaattcag ctgtggctga aaaatggagc ggtttctgtc aatggacagc cagtcaaagc
    26281 aaattacaaa gtaaaacaag gagacgagat tactgtcttg atccccgaac cagaaacatt
    26341 agatgtggtc ccagaaaata tcccgttgac gatcgtttac gaagatgaag atgtagcggt
    26401 gataaacaaa cctcaaggaa tggtcgttca tccttcagca ggtcatacgc atggcacaat
    26461 ggtcaatgcg ctgatgtacc atatcaagga tctttctacg atcaacggcg ttattcgtcc
    26521 aggtattgtt caccgaatcg acaaagatac ttccggactg ctaatggttg ccaaaaatga
    26581 ccaggcacac gaatcattag ctcagcaatt aaaagataag acctctttgc gcaaatatgt
    26641 tgctttagtc catggagaga ttcctcacga aaaaggaacc atcaatgcgc caatcggccg
    26701 ttcgaaggaa aaccggaaaa tgcaggcgat aagagaagat gggaagtcgg ctgtcacaca
    26761 ttttactgta ttggaacgtt tcgaaggatt tactctggtt gaattaaaac tagaaaccgg
    26821 aagaacacac cagatccgtg tgcatatgaa gtatatcggt tatccgttag ctggagatcc
    26881 agtatacgga ccgaagaaaa ctttgaaagg caatggtcag tttctccatg cgcaaatgtt
    26941 aggttttacg cacccgacta caaacgaaaa gatgatcttt gaagcacctc ttccagaagt
    27001 ttttgaaaaa actttagaaa aattaagaaa aaacattgca ttttaattta gataacggta
    27061 tagtaattaa caacgaaagc ataaaactta atagaattcc tttaagttta gtcccgtgag
    27121 gctaagaagg gtgggagcaa ggatacgtat ggtgcgccat aagtagattg ctcattgaca
    27181 tagtatagct atcagtgtat cagtatacgc tcagtctaaa cattcctcct gtcctcttag
    27241 ggcaggagtt tttttatgca aaaaagtagg gggaatgaaa atgcaagcaa aagaagtagt
    27301 agatcaggta accatgaagc gagcattgac tcgaattact tatgagatta tcgagagaaa
    27361 ccatagtatc caagatatcg tattggtagg tatcaaaaca cgaggaatct acattgcatc
    27421 aagaattgcc gaacgcctga agcagctaga agatatcgat attcctgtag gagaactgga
    27481 tatcacttta taccgagatg acaaaaaaga aaatccagaa gaaccggaac ttcattcttc
    27541 tgacatccca gtttccttag aaggaaaaga agttattttg attgatgatg ttttgtatac
    27601 aggacgaacg attcgagcag caatggacgc agtaatggat tttggcagac caagaaaaat
    27661 ttcattagca gtcctggtcg atcgtgggca tcgagaattg ccaatccgtg cagattatgt
    27721 tgggaagaac attccaacat ctcgggcgga agagatttta gtagagatgc aagagcttga
    27781 tggacaagat cgcatcatga ttttgaaaga ggaagattag aggagagaga aacatgtcgg
    27841 agaaaaaagg atttcataat gatgacgtgg tgttagatat ccacgatcgc ccaaccccag
    27901 gacattgggt aggattaagt ttacaacact tgttcacgat gttcggagca accgtgttag
    27961 tccccatctt agtaggtatc gatccaggta ttgcgctagt cagttcagga ctaggtacat
    28021 tggtgtactt atttactact aaaggaaaga tacctgcgta cttaggaagc agcttcgctt
    28081 tcattgcagc gatgcagctt ttgatgaaaa cagatggtta tccagcgatt gcacaaggag
    28141 cagtcacaac tggattagtc tatttgattg ttgcgctgat catcaaaaag atcggttcag
    28201 catggctgga taaaatcttg cctccaatcg tcgtcggtcc agtcatcatg gttatcgggt
    28261 tgggacttgc ggcaaatgca gcaacaaatg ctatgtataa tcagggcacg tatgatttca
    28321 agtatattgc tgtagcgctg atcacattag gattaacaat tttcttcaac atgtggctaa
    28381 gaggttttct cggattgatt ccgattttac taggtattat tagcggatac atcgtagcct
    28441 tgctgtttgg aatcgtggat actaagccaa tcgtagacgc tgcctggttt gctgttccta
    28501 actttgagat tccctttgta caatatgaac caaagctata tcttggagca atcacaacaa
    28561 tggcacccat cgcttttgtg acgatgacag agcatatcgg ccatcttatg gtattgaata
    28621 aactaactaa acgtaatttc tttgaagaac cgggtcttca taagacgttg acaggtgatg
    28681 gattagcaca aatcgtggca gcctttgtcg gaggtcctcc tgtaacaagt tacggtgaaa
    28741 acattggtgt tctggctatc acacgtgtcc atagcgtgtt tgtgatcggt ggagctgcag
    28801 tatttgcagt cattttagga ttcgtaggaa aattaagcgc tttgatttta agtatccctg
    28861 gaccagtcat ctcaggaatc agttttatct tatttggagt aattgccgca agtggaatga
    28921 agattttaat cgagaacaaa atcgattttg ataaaaagaa aaatctgttg attgcttcag
    28981 ttatcttagt agtaggaatt ggcggactcg tctttgaagt aggcacattt acattgtcgg
    29041 caatggcatt agcaactgta cttggcatca ttttaaatct tatcttacca gaaacttcac
    29101 gaagcgaaga aaactaagga ggaaacatta tgatcatcaa atcagaacgt atcagcttaa
    29161 agcatttatt gaccgttgag gcacttactg atcaagaagt tatgggcttg atccgcagag
    29221 ggcaggaatt caagcaaggt gctaattgga ctccgcaaaa gcgccagtac tttgctacaa
    29281 acctcttttt cgaaaatagt actcgcacac ataaaagctt cgatgtagcg gagaaaaaac
    29341 tagggctaga agtgattgaa tttgaagcaa gcacaagctc tgtccaaaaa ggggaaacac
    29401 tatacgatac agtactgacg atgtcagcat taggcgtaga cgtagcggtt atccgtcatg
    29461 gtgatgagaa ctattatgac gaactgatac aaagtaaaac aatccagtgt tcgattatca
    29521 atggaggaga cggaagcgga cagcatccaa cccaatgctt gcttgatttg atgaccattt
    29581 atgaagaatt tggtcatttt gaaggtctga aagttgccat cgtaggtgat atcactcact
    29641 ctcgcgtagc taaatccaat atgcagatgt taaaacgtct aggcgcacaa gtattcttct
    29701 caggtcctag agaatggtac gacgatgaat tcgaagtgta cggacattat atgccattgg
    29761 atgaactact agatcaagtt gatgtcatga tgatgcttcg ggtgcaacac gaacgccatg
    29821 atggaaaaga aagtttctca aaagaaggct accatcagga acatgggtta acagttgagc
    29881 gtgcgaagaa gttgcaaaaa catgcaatca ttatgcaccc agcaccagtt aaccgtgatg
    29941 tagaattagc tgattcatta gtggaaggat tacagtcgcg gatcgttcag caaatgagca
    30001 atggagtatt cgtcagaatg gcaatcttag aagcagtgct aaatggaaaa gcgtaattat
    30061 aggaggatga aaaaatgaaa acactcatta aaaacggtca gatcaataca cgaaaaaata
    30121 tgacgacacc agcagaaatt tggatagaag atgggcgaat caaagcaatc ggtacaggat
    30181 tctcagaagc cgaatttgat gaagtgtttg atgcaaaggg acaattgatc acacctggtt
    30241 tagtggacgt ccatgttcat ttgagagagc caggatttac atacaaggag acgatcgaag
    30301 ctgggactcg ctcagctgct cgtggcggtt tcactactgt atgcgcgatg ccgaatttaa
    30361 atcctgtacc tgatacggca gaaaagctga gacaagtata tgacatcatt agaaaagatg
    30421 cagttgtgaa ggtattgcag tatgcgccaa tcacagaaaa tctgcgaagt gagaaattag
    30481 ttgatcaaga agcactgatt gaagaaggtg cttttgcttt cacaaatgac ggtgtaggtg
    30541 tccaaacagc tgggacgatg tatctggcta tgaaagaagc tgcaaaaaac aataaagccc
    30601 tagtagccca tacagaagat gaatctttat tatttggcgg cgttatgcac gctggcaaga
    30661 aggcagaaga acttggtctt cccgggatct taagcgtaac cgagtcttct caaattgcta
    30721 gagatctgtt gctagctgaa gcaactggtg ttcattacca tgtttgccat gtttctacta
    30781 aagaaagtgt ccgtgtaatc agagacgcaa aaaaagcagg aatccacgta acagcagaag
    30841 tttcacccca tcatcttatt ttgatcgacg aagatattcc cgaagatttc ggcttttgga
    30901 aaatgaaccc gccgctaaga ggacgggagg acagagaagc tttgatcgaa ggcttgcttg
    30961 acggtacgat cgattgcatc gctacagacc atgcccccca tggattagaa gaaaaaagcc
    31021 aaagtttcat gaaatcacca tttggtatcg taggaagcga aacagcgttt caactgatct
    31081 atactcattt tgtagaaact ggtcgattca cgttggaaca agtgatcaat tggttggcag
    31141 ttaaaccggc tgagattttt ggtttgaatg caggaacgct aacggttggt gcccccgctg
    31201 atgtagcagt ttttgatatt acacaaactt gcacgatcga taaagaagac tttttatcaa
    31261 aaggggagaa tacacctttt atcggctgga aagtaaaagg cgaaacacaa atgacttttg
    31321 tcaacggaaa acttgtgtgg cagaagggag aataaaagta tggaacgttt gcttatatta
    31381 gaagatggaa cagttttcga aggaaaggct tttggtgcag atgctagtgt cttcggagaa
    31441 atcgttttta caacaagtat gactggttat caagaaacta tcactgatca aagttttaac
    31501 ggtcaaatca ttacatttac ctatcctatg gtgggtaatt atggggtaaa tagagatgat
    31561 tatgaatcga tttctccaac atgcaaagga gtagtagtaa aagaacatgc acgtgtagct
    31621 tcaaactggc gaaaccaaat gacgctagat tcatttttga aacaaaaagg aattcctgga
    31681 atatcaggta tcgatacgcg cgcccttact cgcaaaatcc gtgaagtcgg aacgatgaaa
    31741 gctagtatag ttgatgctgg agattccttc gaacatgcat ttgatcagct gaaagcaacc
    31801 gttctagcaa ccaatcaagt gcagcaagtc tcaactaaca aaccttatcc aagtccagga
    31861 acaggtcgga aagtcattgt cattgacttc ggcttgaaac atagtatttt acgagaattg
    31921 agtaaaagaa actgtaacct cacagtactt ccctataaca cagatgccga gacgatttta
    31981 gcgctatcac cagacggagt catgctgacg aatggacctg gtgatccaaa agatgtgcca
    32041 gaagcaatcg aaatgatcaa aaaaatccaa ggaaaagtac cgatttttgg tatttgtctc
    32101 ggtcaccagt tatttgcttt agccaacggt gcagatacct ataaaatgaa attcggacat
    32161 cgaggattaa atcacccggt acgagaagta gcgaccggaa gaattgattt tacttctcaa
    32221 aatcatggct atgcagtcga tgaagccacg attgatccag aaaaactcat cgtaactcat
    32281 gtagaagtaa atgacggaac agtagaagga gtcaaacaca gagattatcc cgctttcagt
    32341 gtgcagtttc atccagatgc tgcccctggt ccgcatgatg cactacattt atttgatgaa
    32401 tttatggaaa tgatggacgc aggaaaggag cagtaaacag atgccgaaac gtacagatat
    32461 caagaaaatt atggtgattg gatctggtcc gatcatcatt ggtcaagccg cagaatttga
    32521 ctacgcagga acacaagcct gccttgcctt aaaagaagaa ggatatgaag tggttttagt
    32581 aaattctaat cctgcgacga tcatgacgga taaagaagtg gcagatcaag tatatatcga
    32641 accgatcaca cttgaatttg tatcaaggat tcttcgaaaa gagcgtccag atgctctcct
    32701 tccaacttta ggcggacaga caggcctcaa tatggcaatg gagctggcag catcaggtat
    32761 tttagaagaa ttgaacatag aacttctagg aacaaagctt tctgcgatcg atcaagcaga
    32821 agatcgtgat ctattcaaac agttgatgaa agaattgaat cagccaatcc ccgagtctga
    32881 aatcgtcaat acagtagaag aagctgtaga ttttgcaaat aaaatcggct atcctgtcat
    32941 cgttcgtcca gcctttactt taggcggaac tggtggaggt atgtgtgaaa atgaagaaga
    33001 attacgtatc gtatcagaaa atggattaag tctttccccc gctacccaat gtttgattga
    33061 acgaagtatt gcaggtttta aagaaattga atatgaagtg atgagagatt cggctgataa
    33121 tgcaatcgtt gtctgcaata tggaaaattt tgacccggtg ggtatccata caggtgattc
    33181 tatcgtattt gcccctagtc aaacattgtc tgaccatgaa tatcaaatgc ttcgtgatgc
    33241 ttctctttcg attattcgag cacttaagat cgagggaggc tgtaatgtcc aactcgctct
    33301 tgatccacat agttttaatt attatgtgat tgaagtcaat ccgcgtgttt ctcgttcatc
    33361 tgcgttagca agtaaggcga ctggctatcc gatcgctaag ctagctgcta aaatagcgct
    33421 tggattaaca ttagacgaaa tgaaaaatcc tgttacagga acgacttttg cagaatttga
    33481 acctgcatta gattatgttg tagctaaaat tcctcgctgg ccgtttgata agtttgaaaa
    33541 cggagaacgg cttttaggca cgcagatgaa agcaactggt gaagtgatgg ctatcggtcg
    33601 gaatatcgaa gagtctttat tgaaagctgt gcgttcttta gaaatcggta cacaccatat
    33661 cgaacttcct gagttggcag acatcacgga cgatgaccta atccaaaaag tggttcgagc
    33721 acaagatgat cgcctgtttt atgtagctga agctattaga agagggtaca ccatagaaga
    33781 attagcggat ttgacaaaaa tcgatttatt ctttttagat aaactgttgc atatcgtaga
    33841 aatcgaacag cagttgaaac agcagccgtt ggatgacaag atattgaaaa cagcgaaaca
    33901 aaacggtttt acagacaaaa aagtggcgga gctgtgggaa ataaacgaag aagccgttcg
    33961 ccagatgaga aaagaaaatg atttgattcc tgtatataag atggttgaca cttgtgcagc
    34021 ggaattcgaa tcaaaaacac cttattttta tagttcctat gaatatgaaa atgaaagttc
    34081 acgatcagaa aagccctcaa ttctagtact gggatctggt cctatccgta tcggacaagg
    34141 ggttgaattt gactatgcga ctgtgcattc tgtcaaagca atccagcagg caggatacga
    34201 agcaatcatc atgaacagca atcctgaaac agtttcgact gacttttcta tttcggataa
    34261 attatatttt gagccattga ccttcgaaga tgtgatgaat gtgatcgagc tagaacagcc
    34321 gataggagta atcgttcaat ttggtggtca gacagcaatc aacttagccg aacctttgac
    34381 tgctgctggt gtaaaaatct tagggacaac gatcgaagac ttggatcgtg cggaaaatcg
    34441 tgatttattc gagcaagcgc tgaaagagtt ggatattcct caacctttag gggatactgc
    34501 tacaagtaaa gaagaagccg tggttatcgc ttcgagaatt ggttatccag ttcttgttcg
    34561 tccaagctat gtattaggcg gacgtgcgat ggaaatcgtt gaaaaccagc gagatttaga
    34621 agattacatg gaacatgctg tgaaagcatc tccagaacat ccagtactag tggaccgtta
    34681 cttgcttggt ggtgaatgcg aagtggatgc gatttgtgac ggtgaaacag tactcatccc
    34741 agggatcatg gagcatattg agcgtgctgg tgtccattca ggggattcaa tggctgtgta
    34801 tccacctcaa acattatctg aatcgatcaa gaaaacgatc gaagattaca cgatccgctt
    34861 agcattaggt ttgaattgtg tcgggatgat gaatatccag tttgtcatcc atgaaaatga
    34921 ggtttatgtg atcgaagtca atcctcgagc aagtcggact gttccattct taagtaaggt
    34981 gacaggaatt ccaatggcac aaattgcgac gaaagctatt ttgggagaga aactaaagac
    35041 acttggttat gaaaacggac tttatccaga aacaaaagaa gttcatgtta aagcacctgt
    35101 cttttctttt acgaaattgc agaaagttga tacttactta gggcctgaaa tgaaatcgac
    35161 tggagaagta atgggatctg acagacaatt agacaaagct ttgtacaagg cattcgaagc
    35221 ttctggatta cacttgcctg aatatggagc cgtattattt acgattgcgg acgaaacaaa
    35281 agaagaagca ctagaattag ctaaacgttt tacagaaatt ggctatagtc tgataggaac
    35341 gaaacataca gctgcttatt tcgaaaaaaa tggtctggtc gttgaaacag tagcgaaaat
    35401 ttctgaggaa gcagcagaaa aaaatgtagt cgacttaata agagatggta aaacacaagt
    35461 ggttgtcaat acgattgata aagatcgggg aaatgcttct aaagacggtt tcatcatccg
    35521 aagagaagca gttgaacatg gtattccatt gtttacttct ttagacacag cagatgcgat
    35581 cgttcgagtg atggaatccc gtgctttttc aactaaccct atttaacggc gagcacatgt
    35641 taagacaaaa agacgaaaaa ccgttgcagg aggggacaga tgaaacaaga aatgatggaa
    35701 atcgttcagc aaaaacagct ggcaccaaag atttttgaaa tgacattgaa aggtgatcta
    35761 gtaaaagaaa tgcagcaacc aggacagttc ttgcatattc tcgtgccgcg tgcagacttg
    35821 ctattacgcc gaccgattag cttgaatcat atcgaccacg aaacaaatac ttgccgtatc
    35881 atttatcggg tggaaggtga ggggactaga gtcttctcta ctctttctgc cggagaccat
    35941 ttagatgtca tgggtccttt aggcaatggt tttgatttgt ccaatcttca tacaggagac
    36001 gaagtattta taatcggtgg aggaatcggt gtccctcctc tatatgagtt atcaaaacag
    36061 ttagtcgaaa aaggaattaa gccaacgcat tttttaggtt ttgccacaca tgaagtcgtt
    36121 tattatgaaa aagaatttaa tcgtctagga gaaacaagaa tcgctactga cgatggaaca
    36181 tacggtatgc acggaaatgt cggtaattta ttgttaggaa cgaagacgga acctacagct
    36241 gtttttgcat gtggttcaaa cgggttattg aagacagtgg aacagctgtt ttcttcgcat
    36301 caaaatgtcc agttatcatt agaatcccgc atggcatgtg ggataggggc ttgctatgct
    36361 tgcgtttgtc atacgccgga agatgaaaca ggaacaaaaa gtgtgaaagt ttgtgacgaa
    36421 ggaccgattt ttaaaatagg agaggtaatt atatgaatga gaatccttta gcggtcaaac
    36481 ttcctggcct agaattaaaa aatcctatca tgccggcaag cggctgtttc ggttttggca
    36541 aagaatacgg aaaatactat gatttgaatc agcttggatc gatcatggtc aaggcaacga
    36601 ctccaaatgc ccgctttggc aatcccacgc caagagtggc ggaaacacca tctggcatgt
    36661 tgaatgcaat tggtttgcaa aatcctggtc tagacgttgt tatgcataaa cttcttccag
    36721 aattagagga gtacaatgag ttacccatca tcgcaaatgt tgctggtgct tgtgaagaag
    36781 actatgtaga agtttgtagc aaaattggcg aagcaccaaa tgtgaaagca atcgagctga
    36841 acatttcttg cccaaatgtc aaacatggcg gaattgcttt tggaacagat tctgatgttg
    36901 cttttcaatt aacacaagca gtaaagaaag tttcttctgt tcctgtctat gtcaaattgt
    36961 ccccaaatgt aacagacatc gttccaatcg ctcaggcaat cgaagctgga ggggcagatg
    37021 ggtttacgat gatcaatact cttcttggta tgcggattga ccttaagaca agaaaaccga
    37081 ttttagccaa tcaaacaggt ggactctccg gaccagcaat caagcctgtc gctattcgtt
    37141 tgattcacca agtagcaagt atatccagcc ttccaatcat tgggatgggc ggcgtgcaga
    37201 cagtggatga tgttcttgag atgtttatgg ctggagcaag tgctgtagct atagggaccg
    37261 cgaatttcac cgatccgtat atctgtccta aactaattaa agagctccct gtgcgaatga
    37321 gcgaactagg catcgaatcg ctggaaagat taatcaaaga agtgagagag gaacgtgaac
    37381 gatgaatcaa ctagaccaac gaccaattat tgcattagat ttttcaacta ggcaagaggt
    37441 agaagacttt cttcgatttt tcccgaaaga agaaaaattg tttgtgaaaa tcggaatgga
    37501 attgttctac caagaaggac ctgaaatcgt acgttatcta aaagaggctg gtcataatgt
    37561 atttttagat ctgaaacttc acgatattcc gaatacggtc gaaaaagcaa tgcggggatt
    37621 ggcaaagctt ggtgttgatc tcacctgtgt tcatgccgct ggcggtattc gtatgatgga
    37681 ggcggcaatg cgaggattag aagaggggac tccagaaggt ggaaaacgtc cgctacttct
    37741 agctatcact cagcttactt ctacaagtga agaagaaatg catgccgatc aactaatcga
    37801 agtttcttta gaaaaaagtg tgatccatta tgctagctgt gccaaaaaag caggattaga
    37861 tggtgtggtt tcttctgctt gggaagtaga agcgatcaaa gaaatggcag gagacgaatt
    37921 tgtttgctta actccgggta taagaccaga aggaacagtc gcaggagatc agacaagagt
    37981 agtcacacct tctcaggcaa aaaagatcgg ctcaactttt atcgttatag gtcgaccaat
    38041 cactcagtca actaatcctt ataaagcata tcaaacgatt caaacagaat ggagctaacc
    38101 taaaatgaat gttgaacaaa gtatcgcaaa agacttatta gaaattgaag cagtattttt
    38161 aaatccaagt aatcctttta catgggcaag tggaatcaaa agcccaattt actgtgataa
    38221 ccgaatcacg atgagctatc caaaagtccg aaaagagatc gcaaaaggat tagcaagcaa
    38281 aatcaaagaa gcttaccctg aagtacaagt gattgctgga actgcaacag caggaatccc
    38341 tcatgccgca tgggtagcgg aaatactgga tttgccaatg gtctatattc gaagtaaggc
    38401 gaaagatcat gggaaaggta accaaatcga aggacgtata tttgaaggtc agaagatggt
    38461 tgtgatcgag gatttgattt ctacaggcgg aagtgtcttg gaagcagctg aagcagcaaa
    38521 aagagaaggt gcggatatcc ttggtgttgc ggctatcttt acgtatgagt tgccaaaagg
    38581 aaaagcaaat ttcgaaaaag ccgaaatccc tcttatgacg ttgaccaatt attctgtatt
    38641 gattgaagca gctttagaag atcgttatat cgatgagcaa gaattaactc tgctaaaaga
    38701 atggaaaaaa gatcctgaaa attggcagat tgaagcgcgc ttgatgaaga tagagaaatc
    38761 cgatttgtgt ctccagaatc atcaattgga atagcaaaaa tccaaaaact gattgcgaga
    38821 agcatctttt catcaggtat gaaaagaaac ttctatcgtg atcagttttt gattttttta
    38881 aaattcattt ttctaagact ggttagcaga aagaagagaa taatctcttc tcttttttct
    38941 attatatata gattttctct attttttggt aagggagctg tttcaaaaat ggtaaagagc
    39001 ggaaatgttg cgaatatcag ttatttaatg atatgatatt cggaaaaaga aatctcaaag
    39061 ggaggtaact tttatggggt tgaatgaatt tattttagga ttgaacaatt tagagacgat
    39121 cacaagggca cctggatttt ttaaatatac agagcataca gttgcagcac attcgtatcg
    39181 tgtagcatct attgcgcaag ttctaggaga cattgaagaa gcgaatggac aaccgatcga
    39241 ttggaaactg ctctatgaaa aatcattgaa tcatgactac acagagcgtt ttattggtga
    39301 tatcaaaacg ccggtgaaat acgcaagcaa agagttaaga gggatgctgc aaacagttga
    39361 agaaaagatg acggacgaat tcatcaacca agagattcca gctgagttcc aagaagttta
    39421 tcgccgtcgg ttgttcgaag gaaaagatga aaccgtagaa ggagaactat tagccatttc
    39481 agataaggtt gaccttcttt atgaatcttt tgatgaaatc atcaaaaata acccggaaaa
    39541 agtttataag gagatgttca ttgaggcagt gactacgatt aaaagctttg aacatcggcc
    39601 ttctgttcag tatttcttta aagagatatt tccggaacta ttgaaccagg aattttatgg
    39661 aaaagaggcg tttttagaaa ccgtctcaac gattgtaaag aaagaagaaa aataaaaatg
    39721 atatgagatg cagggggaag tatagttgaa aaagaatatg gatgtcgaat ggggctattc
    39781 tggagacaaa ggtccggaaa attgggcgag tttatgtgat tggtttgcga gaggtgcgga
    39841 atttccattg cagtcgccta ttcatttgac caagggggaa gaaaccaaaa tagaaggaga
    39901 cacgctttcc tttcattatc aaaagcaacg gtttactgac aaggaattca aaaacacgat
    39961 tcatttagta ccttttgacc aactcagtta tgtagagttc aagaaagaac gctattattt
    40021 gacagatatt catttccact tgccttctga acatatcatc aacggaaaac aggaagatat
    40081 tgaatttcat tttgttcata tgaacagcaa aaaagagaat ctagttgtgg gtgtcatgta
    40141 ccagctaatg gtacaggaag gttggctgta taatcaagaa aatggggaaa gttggaactt
    40201 agaaaaacat gagcattggg taaatccgga tgtgttcttt ccggagcaaa aaagccattt
    40261 ccattatatt ggctcattga caactccgcc taccagcggg ccaatccaat ggtttgtaat
    40321 ggatcatgta ggaaaactga accaagagtt tcttctcccc ttcgatggtc agtatgctcg
    40381 accgaacaac cgaccaatcc agcccttgaa tggaagagaa atttattatt tcgaggagtt
    40441 tgagcaatga atattcagca gatgaaatac gtggtcgctg tagcaaacaa tggtagtttc
    40501 agagaagcgg caaagaaaat gtttgtctcc cagccaagtc tttcagctgg tatcaaagaa
    40561 ttagaaaccg agttagggat cacgctgttt actcgaacga atcgaggggc atatttaaca
    40621 gaagaagggc aagacttctt aaatcgtgcc gagaaaatat tggtccagct tgatacatta
    40681 gaaactcact atctaacgag tgaaaagaga gacaattttt cgatcgctgc ccagcattat
    40741 gatttcttag gtcctttgac cgcacagcta ctcgaggaat tcaaagacga gatcaaacaa
    40801 tttcggatca tggagacgac aacagcgaga gtgatcgaag aagtcaaaga gcagcacagt
    40861 gagatcggca ttatttatat gaatgagcat aatcaagctg gtatcagaag atatttacaa
    40921 caaggagagc taagtggccg cagcattgga aaattcaaaa cgcatatttt tttaaggcaa
    40981 ggacatccat tagctgaaca cgcagagatc accaaagaag aattgttatc ttatcctcaa
    41041 gttcgtttta cacaggatgg aaataatttc ccgtattttt atgaagacct tatcgagatc
    41101 cctgatcaag aaacagtgat ttatacgagt gatcgaggta ctttgatgaa tatcgttctt
    41161 gaaactgatg cttacgcttc aggatctggt attgtgattg gagggattcg cgagcacttg
    41221 cggttaatcc ctttggcaga aggcgaacta aatgaatttt atatcattta ttcggcgaaa
    41281 cgtccgctca gtgaaatcgc tcaaagattt atcaaagagc ttactcggtt actggaccag
    41341 cagagctaag aaaagcagat gagaacgctg tttattgaac accgttgatc aaaaaagagg
    41401 ggttgtgaca gaattttgtc acaactcctc tttttgtagc aaacaattta taactaattg
    41461 ctgttgtggg aaagtacgca aatatgattc tagaaacact cattgattac ctgttttgtt
    41521 tcatagctgt aattttttct tcttctggtg tcacgataat cgtcttttgg ttttcataaa
    41581 taacataacc aggcttggcg ccatttggtt ttcggatatg ctttacctgt acaagatcaa
    41641 ccggcacttg ggcagagtat cggtattttg aaaaataggc agctaactcg gcagcttctg
    41701 taattgtttc atctgagggc ttgtcacttt taatgatgac gtgcgaaccg ggtatatttt
    41761 ttgcatgaag ccaatagtcg gtttttttag ctgtcttcat tgtcagctgg tcattttgaa
    41821 gattgttttt accaacaaga atgagcgttc cgtcagaaga aaagtattga tcaggctgag
    41881 atggtttctt acgtttttgt ttttttaagt tcttcttttt caggtatcct tctgaagtca
    41941 attcttcttt aattgcttcg atatccattg ggccggcaat ttccagttga gataagacag
    42001 attcaagata ttggatttca tctttggctt cttggatttg ttcgccaatc agcttaacag
    42061 cgttcttcag cttttggtat cgatggaaat atttctgtgc attttgattt ggtgtcaatg
    42121 ccggatctag tgctattttg attggccgat cttcttcata ataattaggt aaaacaactt
    42181 cttttgcgcc gcgtggtact tgtgtcagaa aagtagtaag tagttcgcca tttctacggt
    42241 aattctcagc gttttcggat tctttgagtg tttgttcacg ttttttcaat ttatttttat
    42301 ttcgttttag ttcattttcg atttttcgaa tcagttctcc gccttgttgt tttgcccgat
    42361 ctttctctgc tttttcgtga taatagctat ctaataattg actgagtgtt ggataagctg
    42421 ttacagcaga tgcctgttcg gacaatacct gataagcaat cggtgtgaaa aaatctttat
    42481 tttccgtttc gtaaaaagta ggaataggct gatggctgat ggctgagaaa aattcgtccc
    42541 atacaaccat cttttcatta ggacgttcca gtaatctttt ggtcagctct tgagcgatat
    42601 cgaaaccgat tccctgaaac tgacgttgga ttgcttttgg atctagttca gtttgggaaa
    42661 gccgctgaaa gattttttct ttctcggagc taaatgggtt caagacctct tgttttggcg
    42721 gagcaacata ttctacacca ggaagcaaag aacgataagt gttttgagag cttccaatgt
    42781 gtttgatcgc atccagtatt tttccagttt ctcgattaac taagacgatc gtactatgac
    42841 gtcccattaa ttcaacgatc aatatgatat tttgtagatc acccaactcg tttcgtttag
    42901 caaagtggaa atggatcact cgatcatttt cgatttgttc gatgtcttct aaaatagcgc
    42961 catccaaata ttttcgaagc atcatcacga agttaggcgg attatcagga ttctgataat
    43021 cgatttgggt aatctgtaca cgagcataac ttgggtgcgc agataataat aatcgatggt
    43081 ttttgccgcg tgaacgaatc accagcacga tttcattttc ataaggctga tggattttcg
    43141 agatccggcc agatagtaac gtttcacgca attcgttgac catcgcgtgg gtaaatacac
    43201 catcaaagga catggttcat tcctcatttc ttttcataag tatattcatt ataacgaaaa
    43261 aaagaagaaa aaccaatttg gccgaatagt aaagcaaaag tctagaaaaa tcttggacta
    43321 attacataaa aaacagtgtt tctatcgttg aaatccaaat ttttagg
//
LOCUS       DASJPC010000015        42487 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJPC010000015 DASJPC010000000
VERSION     DASJPC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.015"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            818..2428
                     /locus_tag="U4A13_000781"
     CDS             818..2428
                     /locus_tag="U4A13_000781"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN1779101.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2720..3589
                     /locus_tag="U4A13_000782"
     CDS             2720..3589
                     /locus_tag="U4A13_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN1779102.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3754..5025
                     /locus_tag="U4A13_000783"
     CDS             3754..5025
                     /locus_tag="U4A13_000783"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1779103.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            5022..6317
                     /gene="rho"
                     /locus_tag="U4A13_000784"
     CDS             5022..6317
                     /gene="rho"
                     /locus_tag="U4A13_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN1779104.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            6416..6679
                     /locus_tag="U4A13_000785"
     CDS             6416..6679
                     /locus_tag="U4A13_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN1779105.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            7124..7573
                     /locus_tag="U4A13_000786"
     CDS             7124..7573
                     /locus_tag="U4A13_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1779106.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7630..9048
                     /locus_tag="U4A13_000787"
     CDS             7630..9048
                     /locus_tag="U4A13_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1779107.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            9048..9908
                     /locus_tag="U4A13_000788"
     CDS             9048..9908
                     /locus_tag="U4A13_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN1779108.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            10082..10855
                     /locus_tag="U4A13_000789"
     CDS             10082..10855
                     /locus_tag="U4A13_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1779109.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            11002..11367
                     /locus_tag="U4A13_000790"
     CDS             11002..11367
                     /locus_tag="U4A13_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN1779110.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11515..12723)
                     /locus_tag="U4A13_000791"
     CDS             complement(11515..12723)
                     /locus_tag="U4A13_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1779111.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12742..13020)
                     /locus_tag="U4A13_000792"
     CDS             complement(12742..13020)
                     /locus_tag="U4A13_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779112.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            13174..13869
                     /locus_tag="U4A13_000793"
     CDS             13174..13869
                     /locus_tag="U4A13_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1779113.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(14311..15108)
                     /locus_tag="U4A13_000794"
     CDS             complement(14311..15108)
                     /locus_tag="U4A13_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN1779114.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(15105..16289)
                     /locus_tag="U4A13_000795"
     CDS             complement(15105..16289)
                     /locus_tag="U4A13_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1779115.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(16314..17561)
                     /locus_tag="U4A13_000796"
     CDS             complement(16314..17561)
                     /locus_tag="U4A13_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1779116.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17776..19521
                     /gene="dnaX"
                     /locus_tag="U4A13_000797"
     CDS             17776..19521
                     /gene="dnaX"
                     /locus_tag="U4A13_000797"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN1779117.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19541..19855
                     /locus_tag="U4A13_000798"
     CDS             19541..19855
                     /locus_tag="U4A13_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN1779118.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19880..20476
                     /gene="recR"
                     /locus_tag="U4A13_000799"
     CDS             19880..20476
                     /gene="recR"
                     /locus_tag="U4A13_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN1779119.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20540..21184
                     /locus_tag="U4A13_000800"
     CDS             20540..21184
                     /locus_tag="U4A13_000800"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN1779120.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            21199..21528
                     /locus_tag="U4A13_000801"
     CDS             21199..21528
                     /locus_tag="U4A13_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN1779121.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21542..22480
                     /gene="holB"
                     /locus_tag="U4A13_000802"
     CDS             21542..22480
                     /gene="holB"
                     /locus_tag="U4A13_000802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN1779122.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22516..23340
                     /locus_tag="U4A13_000803"
     CDS             22516..23340
                     /locus_tag="U4A13_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN1779123.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            23333..23680
                     /locus_tag="U4A13_000804"
     CDS             23333..23680
                     /locus_tag="U4A13_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN1779124.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23749..24621
                     /gene="rsmI"
                     /locus_tag="U4A13_000805"
     CDS             23749..24621
                     /gene="rsmI"
                     /locus_tag="U4A13_000805"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN1779125.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24730..25851
                     /gene="dinB"
                     /locus_tag="U4A13_000806"
     CDS             24730..25851
                     /gene="dinB"
                     /locus_tag="U4A13_000806"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN1779126.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25905..26507)
                     /gene="nrdG"
                     /locus_tag="U4A13_000807"
     CDS             complement(25905..26507)
                     /gene="nrdG"
                     /locus_tag="U4A13_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN1779127.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26822..29011)
                     /gene="nrdD"
                     /locus_tag="U4A13_000808"
     CDS             complement(26822..29011)
                     /gene="nrdD"
                     /locus_tag="U4A13_000808"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN1779128.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            29310..30104
                     /locus_tag="U4A13_000809"
     CDS             29310..30104
                     /locus_tag="U4A13_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779129.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            30094..32136
                     /locus_tag="U4A13_000810"
     CDS             30094..32136
                     /locus_tag="U4A13_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779130.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            32417..32566
                     /locus_tag="U4A13_000811"
     CDS             32417..32566
                     /locus_tag="U4A13_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN1779131.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32577..34091
                     /gene="dltA"
                     /locus_tag="U4A13_000812"
     CDS             32577..34091
                     /gene="dltA"
                     /locus_tag="U4A13_000812"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN1779132.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            34088..35293
                     /gene="dltB"
                     /locus_tag="U4A13_000813"
     CDS             34088..35293
                     /gene="dltB"
                     /locus_tag="U4A13_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN1779133.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            35338..35571
                     /gene="dltC"
                     /locus_tag="U4A13_000814"
     CDS             35338..35571
                     /gene="dltC"
                     /locus_tag="U4A13_000814"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN1779134.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35574..36842
                     /gene="dltD"
                     /locus_tag="U4A13_000815"
     CDS             35574..36842
                     /gene="dltD"
                     /locus_tag="U4A13_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN1779135.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36983..38065
                     /locus_tag="U4A13_000816"
     CDS             36983..38065
                     /locus_tag="U4A13_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN1779136.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            38065..38868
                     /locus_tag="U4A13_000817"
     CDS             38065..38868
                     /locus_tag="U4A13_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN1779137.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38938..39678
                     /locus_tag="U4A13_000818"
     CDS             38938..39678
                     /locus_tag="U4A13_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1779138.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39689..40693
                     /locus_tag="U4A13_000819"
     CDS             39689..40693
                     /locus_tag="U4A13_000819"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN1779139.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40738..42435)
                     /locus_tag="U4A13_000820"
     CDS             complement(40738..42435)
                     /locus_tag="U4A13_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779140.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        13645 a         7435 c         8824 g        12583 t
ORIGIN      
        1 accgtttatc cagaggttgt gagcctacca tttaactccg aatcacaaga aataatttca
       61 aaaacagctt ctcatgtttg atggatattg tgacttgtga ccgaggagtt ggcaggagaa
      121 caccgtttat ttagaaataa ggaggcacga cttttgtcgc atctccttat tttttactta
      181 tgtgattcgt taccttcaat gaatccctac aaggcacttt tgttgaaaaa aaggtcatct
      241 ttgaaaaaat tagtggaatt gttttgtttt tgatattatt taatagaaaa gatatgccaa
      301 aaatccgaga actgtggact acgtttttta cgtaaaggag gtaaataagg gtgaaagaaa
      361 aagattgttt ctacgataag ctatataaac tgttgctgca agtaggaaaa agtagttctt
      421 tgttaggtgg gatattatct atcataatgt ttgttttatt tttttatcta ttatttcact
      481 taaaattttt cagtaacgat tttctgattt gcttaggttt aacgttagct atgtttatat
      541 caagcttatt atgtgggtta aatttttttt gcactcataa aggacttaag aaataatttc
      601 tcaaaggata tcgaaaaaga aaaaattgta agcggatagt attcatttga gtacttgact
      661 ttcccttagt cagcttgtat tatacacctt gggcaccctt ttagagggtc agaagtttac
      721 agaaatttcg gctccctatt cttattgaag aatagggagc tttgtttatt gtctgagact
      781 tttcctgcac ataaattaaa aaggagtttt tgaacttatg acaaaatata tttttgtgac
      841 cggcggtgtc gtttcatcaa tcggaaaagg gattgttgca gcttctttag gacgcttatt
      901 gaaaaaccgc ggcttaaaag taactatcca aaaatttgat ccatacatca acgtggatcc
      961 ggggacgatg agcccatatc agcacggaga agtattcgta acagatgatg gtgctgaaac
     1021 agatttggat ctgggccact acgaacgttt tattgacatc aatttgaaca aatactccaa
     1081 cgtgacgaca ggaaaaatct attctgaagt tttgcgtaaa gaacgtaagg gcgaatactt
     1141 gggagcaact gttcaagtta ttcctcatat tacaaatgag atcaaagaaa aaatcatgcg
     1201 agcagctaaa atgactgatt cagatgtgat cattacagaa gtagggggaa cagtagggga
     1261 tatagaatct ttgccattcc ttgaagcact tcgtcaaatg aaagcagatg tcggtacaga
     1321 taatgttatg tatatccata caacattgat tccttacttg aaagctgctg gagaaatgaa
     1381 aactaaacca acgcagcaca gtgtaaaaga gctacgtggt ttaggtatcc aaccgaatat
     1441 tttggtggta cgtacagagc aacctgtttc tcaaagcgtg aaaaacaaat tggcacaatt
     1501 ttgtgatgtc gaaccagaag cagttatcga gtcgccagat gtagatacac tttactccat
     1561 cccattagtt ttacaagcac aaggaatgga tcaaattgtt tgcgaccatt taaaactaga
     1621 tacaccagaa gcagacatga cagaatggaa agaattagaa gaacgtgtct tgaacttgaa
     1681 gaaaaaagta cggattgcgc ttgtcggtaa atatgtcgag ttaccagatg catatatctc
     1741 agtagttgaa gcattgaaac attctggttt cgcctataat tcagacatcg agatcgattg
     1801 gatcaaagca caagaattaa cgagagaaaa tgtagaagaa cgtttgaaag acgcagatgg
     1861 aatcttggtt cctggtggat ttggtgatcg cggagtagaa gggaaaatcg aagcgattcg
     1921 ttatgctcgc gaaaatgacg ttccgttttt aggtatttgt cttggtatgc agatggcttg
     1981 tgtcgaattt gcacgtaacg tcgttggatt agaagatgcg tcatcagctg aaacgaatcc
     2041 agatacagca aacaatatta ttgatttaat ggctgatcag gaaaacatcg agaatttggg
     2101 tggaacttta cgccttggat tatacccatg taaaatcaaa aaaggaacga agacggctga
     2161 agcatatgat ggagccgatg ttgtgcaaga acgtcaccgt caccgctatg agttcaataa
     2221 caaatatcgt caattgtttg aagaacaagg gctagtcttt tcaggtgtct caccagataa
     2281 ccgtttagta gaaatcgtag agctgccaga gaaaaaattc tttgtgggat gccaattcca
     2341 cccagaattg atttctcgtc caaatcgccc gcaaaaatta atcaaagcat ttgtcggagc
     2401 atcattagca gatcacgaaa caaaataatt tttgaacttg atagaaagag aaaagtagca
     2461 agagaagggg ataagcaatt ttcttgtcac ttttctcttt ttctgatttg ttattatttg
     2521 taggaaaaaa gatagaaagc aaaagacttt tttcgattaa agcgctattg gatattttat
     2581 tttttgcaaa aaacttcttg aaaattttca ttttcattga ttttagtaga aaactaccga
     2641 aatgtttggt aaaatagggt cgttggttaa gaattgtctt aacaaattta gttttacaaa
     2701 acttaggagg aattttatta tgccagtagt atcaggagct gaatttttaa aagctgctcg
     2761 taaaggcggt tacgctgtcg gcggattcaa cacaaacaac ttggaatgga ctcaagcaat
     2821 ccttgaagcg gctgaagcaa aacaagcacc agtacttatc caaacatcaa tgggtgcagc
     2881 taaatacatg ggtggttaca aaatctgtaa agatttaatc actgatttag ttgaagcaat
     2941 gaacatcact gttccagttg ctatccactt agaccatggt gattacgaag ctgctttgga
     3001 atgtatcgaa ttaggctata cttcagtaat gttcgatggt tcacatctac catttgaaga
     3061 aaacttgaaa ttagctaaag acgttgttga aaaagcacat gcaaaaggta tctcagttga
     3121 atgtgaagtt ggttcaatcg gcggagaaga agacggaatc atcggtaacg gcgaattagc
     3181 tgatattgaa gaatgcaaac aaatggttgc tacaggtatc gactacttag cttgcggtat
     3241 cggtaacatc catggtcaat atccagaaaa ctggaaagga ttagcttttg accacttaca
     3301 agcaattgct gatgctgtag gtccagacat gcctttagta ttacatggcg gatcaggtat
     3361 tcctcaagat caaatcgaaa aagctatctc aatgggtatc tcaaaagtaa atgttaacac
     3421 tgaattccaa ttatcatttg ctgcagctac tcgtaaatat atcgaagctg gtaaagataa
     3481 agaaggtaaa ggattcgacc ctcgtaaatt attagctcca ggtaaagcag caatcgttga
     3541 agatgctaaa tctcatatcg actggttcgg ttcagctaac aaagcgtaag catccacata
     3601 acagatacga tatataggaa tggagaacga cttttgaagt cgctctccat tcttttttac
     3661 ttaaaacggc agaccatgct cgtttctttt ttttatctat gataaaatac aagcatatcc
     3721 atgaaaaaag attaataaac aaggtggaaa cagatgaaaa aaatcgtaat caaaggaaac
     3781 cgacctttag ctggtgaagt aacgatcagc ggtgcaaaaa atagtgcagt cgcattgatt
     3841 ccagctgcaa tcttagctga ttccccagtg atcttggaag gtgtgccgga catacaagat
     3901 gttcattcat taattgaaat tttagagatc atgggcgcaa ctgtccattt ttccaacaac
     3961 atactagaaa tcgatcctcg agaagtcgta tcgattccga tgccgcacgg aaaaatcaat
     4021 agtttgcgtg cttcttatta ttttatggga acactgcttg gtaaatttgg cgaagcggta
     4081 gtaggcctgc caggtggctg ttatttaggc cctagaccga ttgacctaca cgtcaaagga
     4141 tttgaagctc taggtgctga agtgacaaat gaacatggtg ccatgtattt acggacagaa
     4201 aataaaggat tgcgtggcaa tcggattttc atggacgtgg tttcagtcgg agcaacgatc
     4261 aatgtgatgt tagctgctgt aaaagcaaaa ggacagacag taattgaaaa cgctgcacga
     4321 gaaccggaaa tcatagatgt ggcaacttta ctgaataata tgggggcaaa agttcgagga
     4381 gcgggtacag atgtgatccg tatcgaagga gtcgaaacat tacatggctg tcggcatttc
     4441 atgattcctg accgcatcga agcaggaacc tatctagcat tagcagctgc agtaggaaat
     4501 ggtatcaaag tgaaaaatgt gatctttgaa catttggaaa gctttatagc aaaattgcag
     4561 gaaattggtg tccatatgaa aatcagcgaa gatgaaatcg aagtttaccc ctcgaaagaa
     4621 ttaaaacccg caaacatcat gacctatcct tatccaggat ttgccacaga tctgcagcag
     4681 ccacttactg cgttactatt gatgactaca ggcacttcag aaatcatcga tacgatctat
     4741 gcgaaacgtg tgaaccatgt gccggaacta gcacgaatgg gcgctgatat taccgtggaa
     4801 ggaaatatga tcatcgtgaa tggtccaaat aaacttcacg gcacagaagt cgtggcttct
     4861 gatcttcggg caggtgcttg tttagtgatt gctggactat tggcagaagg gacaacgacg
     4921 atttacaatg tagattatat cttacgcggt tatgatcata ttatcgagaa actaacagcc
     4981 ttaggggcat caattgaaat gatagaggaa gtgggagctg aatgagcgat tatttgacaa
     5041 tggcagaact agagaacaaa acgttaaaag aagtttatag ttatgccaaa gaatttaaaa
     5101 ttccttatta cagtaagatg aataaaaagg aattatctct agctgtgatt cgtgcacaag
     5161 cagagaaaca aggattttat tatatggaag gaatattgga tatcgttggt caagatggtt
     5221 acggtttttt acggccaatc aattatggac caagtgcaga agatatctat atttctgcct
     5281 cacaaatcag acgattcagt ttgagaaatg gggacaaagt ggcaggaaaa gcccgtccac
     5341 ctaaagaatc ggaacgttat tatggtttga tgcacgtgga aagcgttaat ggaaaagatc
     5401 cggaagaagc aaaagaacgt ccgcattttc cagccttaac acctctttat ccgcaaagac
     5461 agcttcaact ggaaacaaca ccgcaaagac tatctacccg aatgatcgat atcttttctc
     5521 caatcggttt tggtcagcgt ggattgatcg tcgcaccgcc aaaagcaggg aaaacttctg
     5581 tattaaaaga aatcgcaaat ggtatcaccg aaaactatcc ggaagtagaa ttaatcgttc
     5641 tactgattga tgaacgaccg gaagaagtaa cggatctaga acgaagcgtg aaaggcgacg
     5701 tggtatcttc tacatttgat ctgcagccgc aaaaccatac tcgagtttct gaattagtat
     5761 tggaacgcgc aatgcgttta gtcgaagata aacgagatgt cgttatcttg atggatagta
     5821 ttactagact tgctcgggcc tataacttag tcgttccacc aagcggacgt acattgagtg
     5881 gcgggattga cccagccgca ttgtataaac ctaagaaatt tttcggggca gcaagaaaca
     5941 tcgaagaagg tggcagtttg acgattttag cgaccgcttt agtcgataca ggaagccgta
     6001 tggatgacgt gatctatgaa gaattcaaag gaactggaaa tctagaatta caactttcac
     6061 gtgaattatc tgaacgtcgg atcttcccag ccatcgatat caaaaaatca ggtacccgaa
     6121 aagaagaatt attgatgcca agtgaagaat tagaagaaat ttggaaactt cgaaaaaata
     6181 tgactggaga ttcattagaa tatacggaac aactgatccg ctttttgcgt aaaacaaaaa
     6241 acaatcagca atttttcaaa gaatttcaag atgtctcctt tggtaaaaaa aatccgacca
     6301 gaaatctgaa aagataattg caaccttgac tgaaacatgt taagatgtta atgttgtaaa
     6361 tcgacaaatc aactctggtt cagcaaatgg agcagggcaa aggagcgaaa aaatcatgaa
     6421 agaaaacatc catccagatt accacccagt agtattcatg gattcaacaa ctggtttcaa
     6481 attcttgtca ggttcaacaa aaacttcaca agaaacagtt gaatgggaag acggtaatac
     6541 atacccagtc atccgtgtcg aagtaacttc tgactcacat ccattctaca ctggacgtca
     6601 aaaattcaca caagcagacg gacgtgtgga ccgtttcaac aaaaaatacg gtctcaaaga
     6661 cgcaaacgct gcggaataat aaaaacgctg ataactcaac gtttagcaga ctatctgata
     6721 gggatagtct gtttttttat gttttttgcc aatttaaaac ttcgtttgat gctgaaaaag
     6781 tcgtaggatc ataatgcaca aaacaatgaa aaactagtat atagttttaa ttatccactt
     6841 gataaaaaca ccttatatta acgaaatgaa gaggggttat ttcgaagtaa aaaaccattt
     6901 acctaaaatt agaagctaag ctttcctttc aaggaaggct ttttgttttg ttatagactt
     6961 gcgattgttt gtgagctaga tcgttcattt taatgactta tacacgcgga tgatgggtct
     7021 ttttcctctg ttcattcata atattgaacg gtacagctag cgatgctata ctgacctcag
     7081 ttccaagaaa acgttaacat tttttgtggg ggagaggttt tttatgaggt ttgatcaatt
     7141 gatcgcagaa gaactgattg ttttcgatcc atcgatagat tcaaaaggat cattatttga
     7201 gcgaataggg gatgtgttga aaaaaacaaa taaggtaaga aatgttcaaa aagtcgttaa
     7261 ggcactattc aaacgagagg aggagatctc cacaggaata gaagatggat ttgggattcc
     7321 ccatgcaaaa agcaaatatg tgacaaaacc agcactgtgt ttttttcata cagccaaatt
     7381 aacggactat agcgggatag atgaaatgcc aattgaatat atttttgcca tcgtagttcc
     7441 tgaaaaatca gcagatatgc atctagagat tctcagcaca ctctccagac gattgatgaa
     7501 tgcagatttt agaaagggaa taaaagaagc cactacgcca gcagatatca tcagacagat
     7561 tagtcaagaa tgaaaatagt agagtctttg tccgagtatc gatagtgaag aaaaaggagg
     7621 aaaataatca tgaaaatcgt tggtgtaact gcttgcccga ctggaattgc tcatacttat
     7681 atgtcggcag aaaaattaga agtaacagca agaaatctaa accatcaagc aaaattcgaa
     7741 acacaaggtg taaaaacaga aaatcagtta tctgaacaag aaatcaaaga ggcagatgca
     7801 atcattcttg cagtagataa agaaatcgaa ttggaccgat ttgccgggaa aaaagtaaag
     7861 cgtgtctcta caagccgagc aatcaaagaa ccgcaagtag taatagacga agcattgaga
     7921 gatattggcg tattcgttgt gtctaatgaa aaagaaccag ctgcaaatga gaaaaagccg
     7981 acaatctata atcatttcat gaatggtgtg aattacatgc ttccatttgt gatcgcaggc
     8041 ggaatcatca tcgcactgag ttttgccttt gggattacag cggctgatcc caactcagca
     8101 gactataatg tgttagcagc tgcattttca aggatcggag gagatacagc gtttgccatg
     8161 atggttcctg cgttaggcgc ggggattgct acatcgattg ctggaaaagc aggttttgcc
     8221 ccaggaattg ttgccggatt actggcctct acaggtggtt caggttttct tggcggaatg
     8281 attggtggcg tgctagcagg gtatatctgt gattttctgg caaataaaat tagcgtaaaa
     8341 aaagaatttt ctgctattta tcagctgatt gtcgtcccct ttttatcgat cttaattatt
     8401 gggcttttga tggtatttgt gattgaacaa ccgatcacat gggtgttgga tgctttgaca
     8461 aattggctga atagcttggg aaatacatcc gggctattgt tcggattact tatcgggatc
     8521 atgatggcag ctgatatggg tgggccgatc aataaatcga cttcgacttt ctcgatcggt
     8581 ttgatgtcag ctggagtaaa tgcaccgatt gctgcttgta tggctgcagg aatgacacct
     8641 cccctaggta ttgctttagc cactttatta ttcaaacata aatttaccaa agaagaacgg
     8701 acttcaggtc aatcatgctg gattttaggc gcttcgtata ttacagaagg tgctataccg
     8761 tttgcggtat ctgacccgat ccgcgtgata cctagtttga tgacaggttc agcagtcgct
     8821 gcagggattt ccatggcagt aggagttact tcaatggcac ctcatggagg gatttgggtg
     8881 atgctaattc caaatgttat gaacaattta cctatgtatg tcttggcaat tgcatcaggt
     8941 acagtcacta cagcctttat gcttggacta ttgaaaaaaa cagtgaaaga gaaaaaacaa
     9001 gacaagtgga cgaaaaatga aaatctaaca aatgaggtgg aagcgtaatg ttatatacaa
     9061 tgaaagagtt attgaaagtg gctaaagaga atcaatttgc agtaccagcc ttcaatattt
     9121 gcagctatga tatgatgaaa gcaatcatcg aagaagcaga gagattagaa gcaccagtaa
     9181 tcttagaaat tcatcctgat gaaatcgcct atttaagtga tgagtttgtt gcggcagtaa
     9241 gagcctatgc ttatacaagt aaactgccga tcgttatcca cttggatcat ggcggtacga
     9301 tagaagatgt cttacgggcg attcgaaacg gctattcttc cgtgatgatc gatgcgtctt
     9361 tgaaatcatt cgacgacaat gtacagatta cgaaaaaagt ggttgcgtta gctcatgcgg
     9421 taaatgtctc agtggaggct gagcttggaa cgattgggaa taatggttct gctgaaggag
     9481 gtgctgaaac aatcatttat acagatcctg atcaagcgaa gatttttgtg aaagaaacag
     9541 gaatcgatac acttgctgta gcaattggaa cagcgcatgg cttgtatccg aaaaacagaa
     9601 ctccaaaatt aaatatcgac ttactgaaag aattgaacca acaaatcgat cttccatttg
     9661 ttttacatgg cggttcaggt aatccggacg cagaagtcag cgaagcagta aaatacggag
     9721 ttgccaaagt gaatttgagc tcagatctta aaagcgtctt tttcaatgct ttgagagaaa
     9781 tattgagtga aaatccatcg atgtacgagc cgaatgcaat ttatccagca gctaataaaa
     9841 aggtacaaga agtggtcaaa cataaaatgg aaattttgaa cacagtaggc aaggctagtt
     9901 tatactaaaa ataaaaatgt gtggtcaaga gggtatgaca atagtcttgt cataccctct
     9961 taaccacaca cgatgttcag aagtagtttt ttcgaaaaat ttcttcttat ttctcgaagc
    10021 caatcacttc tgtcacaacc tcttttttcc tgttactata taaagtaaat ggggtgattt
    10081 tatgttgaat gaagtggaga atagacagca ggagattgtg aattatctaa aaaacaacca
    10141 atttgcaaga gtcaccgatt tgatcgagtt agtaaattat agcgaagcca cgattaaacg
    10201 tgaccttgtt tttctggaaa aaagtggatt gatccgtaga gttcgcggtg gcgccatgct
    10261 tattgacaat cgaaaaattg acgtgcctta cctgatgaag atcaccaaat tagaagaaga
    10321 aacagaaaaa cgtaagattg cagatacagc ttcttctttg attcgagatg atatggtctt
    10381 attcttggat tccagtacaa cctctttaca cttagtcaga aacctagcaa gatttgatgg
    10441 tttgcagatc attacgaatg gcatcgtcac agctgctatg ttgagtgaat atacaacggc
    10501 aaaagtatct gtattgggag gggcaattgt tccgaaacga gcaacaatca acggttcaaa
    10561 agcctataac gatgcattgt catattatgc ggatttagcg ataatgagtt gtcgcgggct
    10621 agatttcagc caaggagtaa cagaaactca tgaaggagag gcaatggtca aaaaagcttt
    10681 tagaaaacaa tcagaccggc ttgccgtttt agttacacag caaaaaattg cacataaatt
    10741 catgcatcaa gccattgctt ttcatgaaat cgattatctg gtgacaagta aagaactgac
    10801 aaaagaacag tatgcgaaag taaaggaaca ccatatcgaa tgtctgtacg gataaattat
    10861 tacgtgaatg ttctaagtgg cttcctcatt tttagatagg accttatctt tgctcgcttc
    10921 tctcagccga tggtataata atgaaaaggt tatcttgtta gataatctta cttttttatc
    10981 caggagaaag gagtgcagaa aatgaaacat gtgaaggcgt tggtagtgaa ggcaatcatg
    11041 atctgggcga ttctgtgggt cgtgctgaca ggtttatacg gtgttagctt tatggattca
    11101 acgattgttg gggtaattat tgtagtcatg atctatgtgc taggggatct gttgatctta
    11161 cgtaaagtag ggaacatcgc agcgacgatc gcagatgctg gttcagctgc agttatcttg
    11221 tggctctatc tatattttat gaatgataca gtcgatattt ggatgaaagt attgattcct
    11281 gctctattga tcggtgtggc agaatggttc ttccacaagt ggttgctaag tcaaggcatc
    11341 gtaccagatg aacggaaaat ggaataaatc aacagttaaa ctattcttat cgataaatga
    11401 aagctttaaa aataaataga tgtttaatta agaataatca taaactattt ttactcaaaa
    11461 aaccgctcag attattttca gatctgaacg gtttttttta tctattcatc aagatcaaat
    11521 ttcatttttc aatgattcaa tcaattcgcc ctctgcttca tgactatgtt ttcggccatt
    11581 aacaagatag agtgctgtac atatcaatgg aatgactccg gcaatcacat acaatcctct
    11641 aaatgaagta agagatttta gcatccccat caaataagga ccaacgccta agcctaggtc
    11701 taaaccaata aagtaggtag ataaagcaat actgattcga tgttcgcgga cgatttttaa
    11761 acaaacagcc tgtccatttg acataaacgt accataacct aggccgacta atgctccaga
    11821 gataagcaat gtcacactat tcgtcgttac actcagtaaa aataatcctg ctgttaagaa
    11881 taaatagcta ggatacatca cgtattgttc tcctttgaca tcgaaaatcc ttccagtcaa
    11941 tggacgtgta aatgtgatga ccaaagcata tacgacgaag aaaaatgacc cagcagcaac
    12001 taagtggatt acttttgtat aagaagataa aaatgataaa acacttgaat aagaaagacc
    12061 catcaaaaag gcaattcctg taatgaaaag tgctttcttt tcaataaaac tattgatatt
    12121 ccatgagcga agagatattt tttgttcttc agataatgga atatttttta caggaaagac
    12181 aaggcaggca atcgtaacaa agaagaccag tacaattgaa aaccagataa tgaaacgaaa
    12241 atcagtcata ttcagcaaaa tcattcctaa aaatggccca acagcggcag ctaaactagt
    12301 acttaatcca taataattga tgccttcccc ttttttagat gcagggatat atgcagtaac
    12361 gatagcattt gtagctgtag aaactgtacc gtagccgaat ccattcaaga atcgaactaa
    12421 atacaatgcc ccgatagttg gcgtataaag atatgctaat gttgaaagga gatagaagat
    12481 cgcgccgcct cttaaggtaa acttccgacc aaatagttct aactgtttcc ccatgaataa
    12541 gcgtgccagc aatgttccga tgatatagat cccagaagca aaacctgctt ggctcaagct
    12601 ggcgtgtaag gtgtcttgcg caatgactgc catgatgacc atcaacagat aataaatcag
    12661 gtagacaata aaattgatca atgtgattcc aataaaatat ttattaaata atttcttttc
    12721 catgtatgtt atacattccc cttaagctga atatgctttt aatttttgta tttgaacttc
    12781 tacatcgtga taattggatg gatagcgatc aacaagttgg cgaataaata aatctcttgt
    12841 agacataaac agatttaaaa attcttcgca ttcgtttgtt ggattctcat ccactgcttt
    12901 gatcaattcg atcgagtttt gatacgcatc atttaaaaag actaaagaag ctttcatatc
    12961 aaatcttgtt tgatatgctg tttctaatcg attaatgatt gtgttcatcc agtaattcat
    13021 gttttttccc tctattctta aaaaatttta cttctgcagt ataacgaata aaaaaaagga
    13081 aaaaaatcgc ataagtgatg gaaaaaggta cttaacaaat gttaatatca ttagtaaaat
    13141 acctttggac tttaaaaagt tttaaagtac tgtatgatct ttttagatga aagtttggat
    13201 gggggagcat cattggatag tagagtctta aaggaatatt tggataaaaa tgattttccg
    13261 attatacgca aaggatatca taaatatttg acattcgaag ggatggaaga ttcccatacg
    13321 tatatcttga aagagggaac ggtaaaagca agtgttattt caaatgatgg gagagaattt
    13381 aaccttcgct atatcaaaga tcttgaaatc gtctctcttt taaaagacga atattcacaa
    13441 ttcatcgatt cgccgtataa tattcgagtt gaatcagaat atgcagagtt ttatcgaatc
    13501 aatcgtgtgc gcttttggca aaatatcaac caagacccaa agctacaaca ctatgtaaaa
    13561 gagtattacc gctatcgtct gctttattcg atgaaaaaaa tgcagcaaat gctattaaac
    13621 ggaaagctgg gagctgtttg tacgcaactt tacgaattat atgatttatt cggtgtgcgg
    13681 acagaagaag ggatgctgat cgatttttcg atcacgaatg aagaaatcgc caaattttgt
    13741 ggcatctcaa ctgctagcag tgtaaacaga attttaaaac aactgaaaaa agatggtatt
    13801 atcaccgtta aagaccatca tatcgttatt ttgaatatgg acttgttaga agacaatatt
    13861 atcttttgat gaacaaagag gttgtgaaat cagcgttttg acctgagtat tagagaaaca
    13921 aacggaaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga
    13981 ttgaacaccg tttaccaaga ggttgtgagc ctgccgttta actctgagtc acaaggaaca
    14041 atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg aggagttggt
    14101 aggagaacac cgtttaccaa gaggttgtga aaaagctgtt ctgcatcaag taataagaag
    14161 agccaagtaa aaacagtctc ttatgttttt aacttggctg gacttatcac cgtagatgta
    14221 agcttttgaa cgccgtttat ttgcaacaaa aggggctgtg acacgatttt tgtcacagcc
    14281 ctttttttgc gaacattcat ttagcaattt ttagaaactt ctgccgccac cgccaaaagt
    14341 cccgccaccg ctagaatggg tagtacttcc gccaccgccg cttccaggag gtgggctttt
    14401 ggggattctt cttgtcgtga caaaagaatt tgtcagacga tcggtttttt cagttaattt
    14461 gatactggat ttttctcgga atggatattt gtagatgcct gatttcaatt gataacgcga
    14521 aacagtcaca aaataaaaga caagactaag gataagagcc aaaacaacag caatgatgat
    14581 ttctactgtc gtcaaaacct tatagcgtgt aattttgcca gtttcttcgt ctacacgata
    14641 atgtcctcct ggaactccgt cttccacata tgttgctgat ttagacaggt aagcttttgc
    14701 agcattgtaa taggcagcat ttgtcatctg atcataaaca tcatctaaga tcgaatcgat
    14761 tctgctatca gtcaaataat cgatcatatt accagaagtc gaaatgtaga tttcacgctg
    14821 tcccatatct aatagcaata ctgagccatt ttgatcattt ccgactgcgt ctcttaagta
    14881 attatcagca aagtctcttg gttcttctga attagatgta gtcgttacga tcaagactcg
    14941 tcctttgatt ttttcattca gcttttctgc ctgttgttcc aaagattgga tttgatctgt
    15001 tgtgaataac ccagcctcat catttaccgt agctgtatct gcatgagtag ccaccggcca
    15061 gccaacactt actagcaaaa gaaagaataa aaaaaacttt ttcatcatat caagtaacct
    15121 ccgatcagaa agaggatcgc tagcccagcg aaaatcccaa gagcggtcag tcccaatttt
    15181 ttctggctga tcggcaggac accgctgacc tttcctgttt gtccattcat tgcgtagtag
    15241 tagacttttt tacttgagtc attgcttcga taagtaacaa gccataccgg cagtaatacg
    15301 taattattta cttcgtttgt gattgaagca gaagttcgca caccggtcaa tgttgtatat
    15361 ccatttgcgg tatcttttaa cagcttctct gaatagtctt ttaattcaga ttgaatctgt
    15421 gatttgattg cctcatattc gatatctctt ttttctgctt ggaacccggc taaatactgg
    15481 cttttaaaat caattgcttt atctaacggg aaaggttgca cgccttcgac catcttttgt
    15541 tgcgtatttt ttgataaggc attttttact agttctttaa aagaaagctt gcctgctctt
    15601 gaaacagcaa attgcttcgt ttctgtgtat tcgatgtcac ctatccgcca tatgcggata
    15661 actgtaccat tcgcctgcat ggcaccatct acttcagcat ctgtagccca ataagggaaa
    15721 tacacacctg tcaacttatc gatttgatcc ttattgaaaa aagctttggg aacaaaccat
    15781 tttttcttgg tccaagcgag aaatctgttg attgcttctt ctttctcgac agcaaaagga
    15841 agtactttat ttggcaagaa ttctccgctt aaacgtcccg atagtacgac gggattatgg
    15901 cagtagtaac aataagtcgc agcagttgtc gcatcagtca cgatctctgc accacagttg
    15961 ggacaaagaa aaagatctat cgctgcggta ttatctgcag attcgtccgc tgtatctgaa
    16021 aaaccgcctg cctcgtcaac cgctttgcgc tcggcttcat tcatatcggc aagctgttct
    16081 tgggctgtaa aagtaaaatc ttcggtaggt gaaggttctt ctttgctttc ttcgccagcc
    16141 atacgcgctt ctttttgctt ttgctcatat tggctgactt cttcttctgt atagatattc
    16201 aggcagtatt cgcaatgaaa tttttggtct ttgggatcaa aagtcaacgg accgccgcaa
    16261 ttaggacatt tatgtgtaag aacatccatc agctgcacac ctttctaaat aaattaatta
    16321 acggggattc ctttaaacgg tttaccacag tttggacaga atttagggat gccgttggaa
    16381 agatccacga tctcattgca ttcactgcat cgcatttgta gcttaggctg aacgttttct
    16441 acaggtttgg cagctccgca gttggaacag aattttccgg aattttctgt cccgcaaact
    16501 ggacatgtcc aagtatccgc atttccttgt gcagtttgtt gattttgttt ttctgcttgt
    16561 tgtttgattt gttcttggtt attttgagct gcttgagaaa gataagaacc atttgcattc
    16621 attcccatac ctacacccat aaagccggtc attgctcctg cttcattttt tccggctgct
    16681 tccataccac gtgcaatgga accttgcacg tatccttcac gtacgctagg atcacctagc
    16741 atcgcccctt cgttacgcat attgatcagt ttcacagaat catcggtgta agaaatgctg
    16801 gctacagcca cagaaacaat ctccatccca cgtaattcac gccaagaatc atccaatgca
    16861 gtatccatgt atttgcttaa ttccaaactc ttagatggta cataagaaat ccgttgacca
    16921 tctgcagaca tctgattgat tgctgattgt agcgctgtta ggaattcagc taaatactgc
    16981 tcattgatat cattgatttc cacttgtgtt ttgtttttag ggattgcgtt tgtataaaat
    17041 aaaatcgggt caacgacatg aatagaataa gtgccatgtg cccgtaagaa taattctgcg
    17101 ttatagaaat tgtcaaagta gttcaacggc gcgcttgtac cgaatttgat tccctttatt
    17161 tcttggagat tgatataaaa aacttgttgt ttctgcggtg tgacgccgcc aaatttgaat
    17221 cggtcaaaag tttcagcaat tgcttctttc agtgttccat tgaacatgga aggagctgcg
    17281 tcatttttaa tggtgtaata accttcctca gcggtataat cgatgatttt tccaccatcg
    17341 accaaaagca tcatcatgtt tggatagaca tggacgactg ttccgtctgt taaaacatct
    17401 tctgttcctt tacgattgga gccacgtttg tcatctttgc ggactttgac accttttgtc
    17461 atgaccgttg tatcactcat attgtctggt tcgatgactt ctagccattg atctgccaga
    17521 ccgccgccaa ccatacttgt tgctgctttg ataagaccca taaaaaattc ctcctctgat
    17581 tcagctagtg ccaaacaatt attgtttatt tattatacca taggaagatg tgaaacttct
    17641 cggactagag acggaattga aatggaaaat tgcgtattgt ttaaatacct gaaaaagccg
    17701 agaaggatag tagttttttt acctaatatc acgtataata agagatagtg aattttttaa
    17761 aaaaggggga tccttatggc atatcaagca ctttatcgag tctggcgttc gcagcgtttc
    17821 gaagacatcg ttggacaaaa agctgtgaca cagacactaa aaaatgctat tgtttcccac
    17881 aaaacctcac atgcctactt gtttacagga cccagaggaa ccggaaagac gagtgcagca
    17941 aagattttcg caaaagcaat caactgtccg aacagtaaag acggtgagcc atgcaacgaa
    18001 tgcgaaatgt gtcgatcaat tactgctggt acgcaagaag atgtgatcga aatcgatgct
    18061 gccagcaata acggagtcga agaaatccga tttatccgtg atcgggcaaa ttatgcgcct
    18121 acaaaggcag cgtacaaagt gtacattatt gatgaggtac atatgctttc aacaggagct
    18181 ttcaatgcct tgttgaaaac attggaagag ccaaaagaaa gtgtcatatt catcttagca
    18241 acgaccgaac ctcacaaaat accagctacg atcatttcac ggactcagcg atttgatttt
    18301 aaacggatca atacggcaga tattgtcgaa catcttgaat ttatcttgaa agaatcgact
    18361 atcagctatg aaccagctgc attgaatgtg attgcccgtt ctgcagaagg cgggatgcgg
    18421 gatgcattga gtattcttga tcaggcaatc tcgtttagtg aaggaacaat tactttagat
    18481 gatgccatgc aagtgacagg tagcttaact gacgaaatga tggacagtta cttgagtgct
    18541 tgcgtgaaaa aagaagtacc agaagcattg gaaacattga accaaattct cgcagccggc
    18601 aaagaatcac gtcgctttct agaggatatg ttacaatact gccgtgactt gctgatgtat
    18661 cagcaagcac ctaacctagt tgaggaacaa tcgagtacga tcagaagtac atttacagaa
    18721 ttggcacaaa ctgtttcagc ggatcagctg ttttcaatga taaagatttt gagcgatact
    18781 caaaatgaaa ttcgttttac caatagtgcc gctatttatt tggaagtcgc tacagtcaaa
    18841 cttgcacagc taccgtctgc ttctgttcct tcagcagatg tagcatttgc agatgatcag
    18901 atagtaaaac agctacaaca agagattaag gaactaagaa acgaaatcaa ggaactacgc
    18961 agtaatacag gaattgagaa tacaaaagta cagccggtac agcaaacaaa tcataaaaaa
    19021 cagacaagta cctatcgtgt accgacggaa cgagtatata aagttctgcg ggaagcgaca
    19081 agacagcatc tgaaccaagt aaaagaagta tgggaagact tgctgatgag tctatctgtc
    19141 actcagcgag caatgcttaa agcaagcgaa ccagttgcag ctggacctac aggactagtg
    19201 attgcgtttg attatgagat tgtttgtcaa agagcagcaa atgacgaaga attgcagctt
    19261 tcggtacaca acaacttaag ccgaatgatc aaagattatg cgcctgatcc tgtttatatt
    19321 acaagagaaa gctggccaga acttcgaaga aattatttga cgcaggcaca agaagaggga
    19381 acagaaaact cttttgcttc tgaagactca gaaggagaaa tcgagttgct tcctcaagaa
    19441 caagaagtac cagaagaaac acttgtagta aaagcagagg aactgtttgg ctccgaagct
    19501 gttacagtgg tagatgatta ataaaaagga gtagataatt atgatgcgtg gaatgggcaa
    19561 tatgcaaggc atgatgaaac aagtacaaaa aatgcaaaaa gaaatggcag aagcacaaga
    19621 gctgctaaat aaaacagagt ttacaggtaa agccaccaat gaattggtca aagcggtttt
    19681 tacaggcgat cgtcgcatga aagatatcca aatcaatgaa gaagtcgttg atcctgatga
    19741 tatcgagatg cttcaggatc ttgtcgtaat ggcagtcaat gatgcgatcg cacaaattga
    19801 tgtggaaact gagaaaaaat tagggaaata cgcaaaaggt ttgccaggat tttagtttta
    19861 aactgaagga gtaatgaata tgcaatatcc agaaccaatt gcgaaactga tcgacagcta
    19921 catgaaactt ccaggaatcg gtcagaaaac agcgacaaga ctcgcctttt atacgatcga
    19981 tatgaaagat gaagtagtaa atgaatttgc taaatctttg ttaagtgtaa aacgcgattt
    20041 gcatttttgt agtatctgcg gaaacatcac agaagaagat ccctgcgaaa tatgtagaaa
    20101 tccttcacgt gatcgaagcg ttatcctagt ggtcgaagag ccgaaagatg taatggcaat
    20161 ggaaaaaatg cgagaatatc acggacttta tcatgttctc catggtgtgt tatcaccgat
    20221 ggaagggaca ggtccagaag acatcaatat ttcttctctg ttgcagcggc tgcatgatgc
    20281 agatgtaaaa gaagtgatta tcgcaacaaa tgcaacaaca gagggagaag caacagctat
    20341 gtatctctca cgtctgatca aacccgcagg tatcaaagtg actcgtctag cacatggtct
    20401 gtctgtaggc tctgatatcg agtatgcaga tgaagttact ttattgaaag ctgtcgaagg
    20461 aagacgtgag ttataaattt ctaattaagt gttttttcag atacaatata aaagcagcac
    20521 aagagatggg ggcaacaatg tgaacggact atttattaca atcgaggggc ctgatggtgc
    20581 aggaaaaacc agcatcttga atgaacttta ccctcttttg aaagaagtag caaaaacaga
    20641 aatcattcaa acaagagaac caggaggcat tccgattgct gaagagatcc gagcagtgat
    20701 ccttgatcca aaaaatgatc ggatggatga acgtacggaa gcactgcttt atgcagcagc
    20761 aagaagacaa catctagtcg aaaaggttct gcctgctctg gcaaaaggga aaatcgtttt
    20821 atgcgataga tttgtcgaca gttctcttgc ttatcaaggt gcaggccgac gcatcggcgt
    20881 taccgagatt gctcggttga atgagtttgc tacagaaggt actacgccgg attttaccct
    20941 ttatcttgac gtagattcag acaccggatt aagaagaatc aagaaaaaca gacaaaatca
    21001 aatcgaccgt ttagattcgg aaggtttgga attccaccag cgtgtacgtc atgcttactt
    21061 aaagctggca gaagagaatc cggaaagaat ccacaaagtt gatgcaagaa aaagctttga
    21121 agaagtgctg caaacaagtt atcatacaat tattgaacag tatccgcaat tttttgaaaa
    21181 ctaggaaggt gaacgtttat gaaaattatt ttagcgatta tccaagacaa agacagcaac
    21241 cgtttatcaa atgaattgat tgatgcaaat atccgtgcca caaaactatc atcgacaggc
    21301 ggcttcctta aggctggaaa cagtacgttt atcgtgggta ttgaagatga ccgtgtagaa
    21361 gaagcattgg aaatcatcaa aaaaacgtgt gaatcaagaa agcaatttgt atctacacct
    21421 gttacactgg atatttcgat ggatgggggc gtcccttatc cagtagaagt agaagtaggc
    21481 ggtgccacag tcttcgtctt acctgttgaa ggattccatc aattttagga ggagctgagc
    21541 tatgaatcaa gaaataaact tggctcaaat gcaaccgatc gtgtatcagc agcttcaacg
    21601 gagttttgag catgggcgta tcgctcatgc ttatcttttt gaaggggaga aaggaacagg
    21661 aaaacatgaa gtaggtatat ggcttgcaca gcacctgttc tgtacccaaa tgaaagacaa
    21721 tctgccttgt ggaaaatgca acaattgtca gcggatcgaa aagaaagaac atccagatgt
    21781 tttggttatt gaaccggaag gacaaaccat caaagtcgat cagatcagac gtttgcagac
    21841 ggaattcagt cgcagcggtt acgaatcaag aaaaaaagtt tttttgatca aagaagctga
    21901 aaaaatgaat agcagtgcgg caaacagttt gttgaaattc ttggaagagc cccctggaga
    21961 ttttttagcg gtgttagaaa cagatgcagt aggaagaatt ctccctacta ttcaatctcg
    22021 ttgtcaagtt ctgcattttc aagaactttc gaaagaagca ctgatccatc gattgcaaga
    22081 agagcagatt ccattggaaa aagcaaaact tttggctttc ttaacaaaca gtttaggaaa
    22141 agcagttgaa atctctcaag atgaatggtt taatgatgct aaggacttga tttatcaatg
    22201 gttcgtttac ttgcaaaagc aagatactca agcctttatc tatgtccaaa aaaaattggt
    22261 aaaatcattt aaagaaaaaa cacaacagtt tacaggatta tctgtcttga tgttttatta
    22321 tcaggaagca cagcgtcaag cagtagcaaa cggcgaatat ggcaaagtga aacagataaa
    22381 ccagataatt gaacggatat tgatcgcaga gcagaaacta cgcagcaatg tcagttttca
    22441 ggctgttgcg gaacagttcg ttttgcatac gattttttag cttgccgcaa aggatgatga
    22501 tcaaggagga tggaaatggt agaagtagta ggtgtccgat atagagatgc aggtcatatc
    22561 tattattttg caccaggaga ctctgaatat gtttatagtg acaaggtatt agtcgaatct
    22621 cagcaatcct tacatattgc tactgttgtc cttcctaaaa aagagattga tcccaatgac
    22681 cttcctgaag ctatccgtcc gatcttgcat aaagcgtcac aagctgaatt agaaaaagac
    22741 aaaaaaaaca aaaaagatgc ttatgatgca ttgaaaatcg caaaagaaaa agtgcagttg
    22801 cgtgagttgg aaatgaaaat ggtcaaagcc gaatacacat ttgatcgaaa taaattgatt
    22861 ttttctttca cagcggacgg gcgaattgac tttagagaat tggtaaaaga tctggccgct
    22921 gtatttcgta ctcgtattga gcttcgacaa attggtgtcc gcgatgaagc aaagataata
    22981 ggcggaattg gtccatgtgg gcgtccatta tgctgttctt cttttttagg cgatttcgtg
    23041 cctgtatcaa tcaaaatggc aaaagatcaa agtttatctt tgaatccaac aaaaatttcc
    23101 ggtttatgcg gacgcttgat gtgctgtctg caatatgaga gcgaagaata cgagacagcg
    23161 aaaaaagaac tccctgactt tggaaaagta attgctacac ctgatggtaa agggaaagtc
    23221 gtagggttga atctgcttag ccgtatcgtt agtattcggc tgactggacg ggaaaatcct
    23281 gtcgaatatg aatgggatga attgaaagaa tttatccaag taggtgagat gaatggataa
    23341 gagatcatta tacgacggat tgaatcaatt agaatcagag ttgagaaata cattaacaga
    23401 acttgttgaa atgaagcatg ctttgcatga gatcgtagaa aaaaatacaa ctttggagat
    23461 ggaaaatcaa cgtctgcggg aacacctgcg ggaattgaac caagttgcac aagctccagc
    23521 tgaaaatgtg aagcaggaac tttctaagtc tcgtatgaat cttgaaaaaa tttatgaaga
    23581 aggattccat gtctgctacg atttatatgg atcaagaagg gacaatgacg agccttgtgc
    23641 gttttgtcta gaagtgatct atcgcgaaag cggtcgatga ccaataacaa gaggagactg
    23701 gagcggagaa atactgctcc agtcttttta ttaaggagat tgtgacagat gtataatcaa
    23761 aaaagtttca agggaaatcc ggtatatggg gatttatatc ttgtaccgac gccaattgga
    23821 aatttagaag atatgagcta cagaagtgtt cgtatgctgc aagaatccga tcttatcgca
    23881 agtgaggata cgcgtaatac acaaaagtta ttgaatcatt ttgaaatcca tacaccccaa
    23941 aaaagtttac atgaacataa ttataaagaa cgtgtccctc aattgattgc tttgttaaaa
    24001 gaaggaaaaa cgattgctca agtcagtgac gcaggaatgc cttccatcag cgatcctgga
    24061 catgagctag ttttagcctg cataaaagaa gggatttctg tgatctctat cccaggacct
    24121 acagctggga tgacggcttt gattgcttca gggctagtgc ctcaaccatt tttattttat
    24181 ggcttccttc cacgtaagaa aaaagaacag aaagaaacat tgaaaaagct aaaagaacag
    24241 acagttacgc tgatttttta tgagtcacct tatcggatca ctgctacatt gacgaatttt
    24301 ttagaaattt ttggaaatcg ccaagtcgtt ttgtgtcggg aattgacaaa ggtccatgaa
    24361 gaatacctta gaggaagtac ggaagagcta ctcgcttata tcgaagaaca tccagtaaag
    24421 ggtgaatgtt gtctgcttgt agaagggaat ttgttcgaaa tgccagtgac ggaagttgat
    24481 gaagagttgg gaacgttaaa ggaacaagta gaagcaaaga tcgcttcagg cgaaaaacca
    24541 aatgctgcta tcaaagcagt agcgctgaaa aatgggctga aaaaacaaga agtctaccgt
    24601 cagtaccatg aattagactg agatagtata agttgtctat aatgaaagac taatgaggaa
    24661 atcagctact aacttttcaa atctcttgaa cgcgaacgaa tgttcttgta taatgaataa
    24721 gaggtgagga tgatggagct gcaatttcct ttacataatg atacatcaag aaaaatcatt
    24781 catattgata tggatgcttt tttcgcttcg gttgaagaac gagatcatcc tgagtttcgc
    24841 ggacatccat tagtaattgc cagacacccc agtgacactg gtgggcgtgg agtagtgact
    24901 acagcaaatt atgaagcaag aaaattcgga attcattcag caatgagtgc acaaaaagcg
    24961 tatgagcttt gtccgcaagc catctttaag cctggcgacc atcataaata cagcgatatt
    25021 tccaaacaag tacgggaagt attctatcgt tatacagata ttgtggaacc agttagtatc
    25081 gatgaagcgt atcttgatgt gaccgaaaat aaaatcaatt gcaagtcagc catcaaaatc
    25141 gcccgaatga tccaagcaga tatttgggag agtgtccagc taacatgttc tgcgggagta
    25201 agttacaata agtttttggc caaattagca tcagactatc aaaaacctag aggaatcacg
    25261 gtagttgccc ctcaagacgc tgtgccattt ttaaaagctt tgccaattga aaaatttcat
    25321 ggggtaggga agaaaactgt acctagaatg catgagctag ggatttatac aggaaaagac
    25381 ctatatgaat gcacagaaat gatgttgatt cgaaattttg gaaaaatggg ctattctctt
    25441 tatcgtaaag tcagaggaat ccatgattct ccagtaaacg ttacaagaga gcgaaaatct
    25501 gtgggaaaag agcatactta tgggcagccg ttgcaaacgg aagaagcagt gctgactcag
    25561 ttgcgccaac tagctgaaaa agtagaagaa gcactgagaa gagtccaaaa acatgggaaa
    25621 acagtagttt tgaaggtcag gtacacggac tattctactg tgaccaaaag agtgacattg
    25681 ccagaatata tctataaaaa agaagcactc ttttatcaag caagccttat atgggaggaa
    25741 atcttgggag tagaaaaagg aatccgattg ttagggatta ctttgacgaa tctggacccg
    25801 atgacttatg aaaacatcgt ccttccatta tgggaaaatc aagaaatata aaaacagctt
    25861 tgagagttga aaccggaaat ccgttttctc tcccaaagct gtttttatat caagtcttgc
    25921 tttttgattt gttcataggt ttctgttgca tccgtacatt tttcccaaat cacaacttga
    25981 ttggattcta aagatttttg gacgtcaatg atccgctggt tgctacttcc gcgaaattgt
    26041 agttttaggt cacgtttgct taattcaaag cgtccgtcaa caaggatatc gatttgcgaa
    26101 aggagttcta atttatcttc tgaatcttgt agcaattcat caaaggcata gcctgtccat
    26161 gaccagatat cttttgtcgt accgaactct tttcggatac gtcgtacgac ttgcagacaa
    26221 acatctgtat tcaaaaaagg ttcgccacca agtaaagtta atccttgcac gtaatcgttg
    26281 cgcaagtcag cgatgatttg ttcttctaat tcaggtgtga atggtttgcc atatcggaaa
    26341 ttttgtgcag ctttgttgaa gcagccttcg caggcaaata agcagccact aacgtaaaga
    26401 ctgttgcgga caccttcacc atcgacaaaa ttaaacggtt tgtagtcagc aatatagttt
    26461 tggctgtagt cttttgctaa ccattctttt ggttttggat ttctcataaa aaaacttctt
    26521 tcatttaagg ttcaaagata tagctattga agataaaatc tactcgacca cataaataag
    26581 aacaaactga cctaaatcca ttgttcaaga aagcttgttc ttattttgat agagggcgtt
    26641 caaagataca aagccatcga aagagggtgg tgaaaaaaga cttgtcatag cctcttattc
    26701 tgaataaacg gtgttcaaaa gcttgcatct gcagtaataa gcccaactaa acgaaaaata
    26761 tgaggaacta tttttgttta gctattctta ttacttgatg caggacagct ttttcacaac
    26821 ctcatttcat gtgtttcaca cgtgaggaaa tttctttatg gcggccatgt accatcggac
    26881 gagcttgcgg atttcctaaa tagccacaag tacgtttgac tacatcacat gtttttggat
    26941 catgatttcc acattctgga cattcgaacc ctcgttctgt cggtttgaaa tcaccttcga
    27001 aaccacattg atagcaatga tcgattggcg tattcgtacc aagatacccg attttgtcat
    27061 atgcaaaatc ccatacagct tctaaagctt tcggattttg ttggagcata gggtattcac
    27121 agtagtggat gaatccacct gaacaatatt ttggataatc cttctcaaat tccaattttt
    27181 caaatggcgt tgggttttta cgcacatcat agtggaaact gtttgtataa tattctttat
    27241 ctgtgatatt ttcgatgatg ccgaattttt ctgtatccaa acggcagaaa cggtcagtta
    27301 gactttcact tggagttgaa tagacactga aatgataacc atattcatcc ccccatgcat
    27361 ctgcattagc ttttaattct ttgacgatat caagtgtgaa agcttttgct tctgggtttg
    27421 tttcccattc gccaccgaag aaagctgaag caacttcata caatccaatg tagcctaacg
    27481 atacagtagc tcgacggttt ttgaataatt cattgacaga atcggtacgt gaaagacgtt
    27541 tcccgaaaga accatacata tataaaattg gcgcatttgc tggaatggct tctttacaac
    27601 gttctacacg atagagaagc gcatctttca tgatttctaa gcggtcagac agtaactgcc
    27661 agaatttatt ctgatctcct tttgattcga tcgcgattct tggcaaattc aatgtaacaa
    27721 cacctaaatt cattcgtccg acatttactt cttgaccatt ttcgtctttc cacccttgca
    27781 agaatgaacg acagcccata ggtactttga aacttccagt taattcaacg attttatcgt
    27841 aattcagaat atcaggatac attcttttcg ttgcacattc caatgctagt tgtttgatgt
    27901 cgtaattagg atcttcaggg tttaagttca cacctttttt caaagtgaag atcagtttag
    27961 ggaagatagc tgtccgcttt tcactcccta atccattgat tcgaatctgt aaaattgctt
    28021 tttgaatttc tctttcaaac cagttttctc ctagtccaaa tcctaatgaa gtgaaaggag
    28081 tttgtccatt tgaagtaaat aaggtattga tttcatattc caaactttgc atagcatcga
    28141 agatatcttt tttggttttt gcttttgcat attctttttg gcgttctggc gaatcgatcc
    28201 attcttccgc gtcttttaga tgttttttat agttcaattc tgcaaaagga gcaagcaatt
    28261 catctgttcg gtcggcagaa cagccaccat attgacttga cgctacattg gcaataatct
    28321 gtgagatttg tgctgtggcg gtctggatag attttggcga ttcgacttcg gcattgccga
    28381 ttttgaaccc attgttcagc atgcctttga aatcaatcaa gcagcaattc gtcatcggtg
    28441 tataaggatg ataatccaaa tcatggtaat ggatatcgcc tttttgatgc gcatttgcta
    28501 tatgtggtgg caacattttc agtccgattg attttccaac gatcccggct gtcagatctc
    28561 gctgtgtatt gaaaacatta ctgtctttat ttgcgttttc gttcacgaca gtttggtcct
    28621 tgttgattag cttttcaatc gaaaagttga tatctgttgc tttactgcga gcaaaatctc
    28681 gtttggtacg ataattgatg tactcttctg ctaattcata ttcgtttttt tctagtaata
    28741 tatgttccac gatgttttga atctcataga ttttgacatt ttcagcaaac cggcttttga
    28801 tttcttggac aaccaagtct gcaatcattt gaattttttc ataagtaagt ggtgataatg
    28861 agccgtgaat tttcttttct gctttgatca gtgcttcatt gatcttttgt tcatcgaaat
    28921 caacatggcg gccatcgcgt ttgatgactt tcatctggga tgtctctggc tgctgagtaa
    28981 cgacagtttt ttcttcataa tttactttca ttatcttcaa ctcctgctaa attgttcttg
    29041 tctatcataa aacaaaatcg gggaaggagc aactaaatat cggctatata tagtattcaa
    29101 tgtgctgtat aattggattt ctatactata tgtagtgtat attttattgt gaaaaaaggt
    29161 gaaaacttgt agctggtgga tttgtttttt gcatatttaa ttttgctgaa taaaataatt
    29221 gaataaaatt ttttattcag tccaagtaaa agtgacaaaa acataagagc aaattattcg
    29281 tagatatgct actatgcaaa taaatagaaa tgaaaaatga aagaggagga gaagaattgg
    29341 aaaagatgat cgaagtgaaa catctaagta aaagctacgg cagcaaactt tctcccgtca
    29401 aagccttaga taatatctca ttcacagtag aagaaggcga gtttgttggg attatgggcc
    29461 caagtggtgc aggaaagaca acgctgatga atatcttgtc cacgatcaat ttgcctacga
    29521 tgggcagtgt ctcgatccaa ggaaaagaaa tcacaaaaat gaaaaacagt gagctcagtg
    29581 actttcgtcg aaaaaaacta ggattcattt ttcaagagtt caatttgatc gataccctaa
    29641 atgcaaaaga caatatccta ttgcccctag cagttgaacg gatgactaaa gaggaaatgg
    29701 aaaagagagt aagacatgta gctcaattgt tgaatataga agaactgctc aaacggtatc
    29761 ccgatgaact ttctgtcgga cagagacaac gaattgcagc tgctagagca cttgttgttc
    29821 agccacaagt gatttttgca gatgaaccaa ctggctcttt ggactcgaag tcggctacag
    29881 aattgttgaa ctacttgaag accatgaatc aaaaagaaaa agcaacgata ctgttagtca
    29941 cccatgatcc atatacagca agctactgcg atagaatttt atttattaaa gatggtgtga
    30001 ttttttctga agttgtcaga agagggacaa gaagagagtt ctttgagaaa gtaatagaca
    30061 tgcaggcgac gattggcgga ggaggaaaaa tgaatgctgt ataaactagc cgttaaaagt
    30121 ttttttcggc agtcacgagg ctatcttgtt tattttttca gtttgacttt atccgtaatg
    30181 atctattatt ccttcagcgc aatgacctac gatcaatctt tagtcagaag agcaagtcaa
    30241 gatacttcga tagatggtgg attgagtttt ggtggattta tcattactgt tgttttgctt
    30301 tttttcgtat tcagtgccaa ccgatttttc ttgaatagaa ggcaaaagga aataggcatc
    30361 tatcagcttt ttggtatgaa taaactgcag atatcaggta tatatgtgtt agaaatcatg
    30421 ataattggat tgttcgcctg tatttctggg atattactcg gtattatttt ttccaaactc
    30481 ttttcaatga ttttggttcg aatgatggat atggatctta ctagtccatt ttttatctcc
    30541 attccatcag tagtagatac tttatttgct tttttcatca tccttttagc agtatctgta
    30601 tattcaatat ggaaaatatg gcgctatcca atgattcgaa gttttggaga aaaagaacaa
    30661 gcagatagtt caacaatgcg cttcaggaca aggcaccgat tactcgcagt catcgggtta
    30721 ctacttttaa cgagcggata ttttggagct agtcatttta gggaaatcac ttcatggatg
    30781 atcagttatc attatcttga tacattagtt ttcttttttc cttttttaat ttttttcgtt
    30841 tgtgtggtcg gtacttattt gtttttctgt tatacatttc ggttattttt gatcatattg
    30901 agtaaatgga agatttatta ctatggtatc aattttttga taatgggtaa cacgcaagtt
    30961 catttgctca aaagttggcg tacaaattct ctgattacag tcattctcgg aatttcttta
    31021 atgacaattg gcggcacggc tagtatctct actattttat cctaccagga gaaagtttcc
    31081 tccccgatgg actatcagtt tgatgttgag acagagaaaa aaatcaagcc gattctagca
    31141 gaggaagggc agaagataac taaagaaatc accttgcatt ataaagtcat tgggagtatc
    31201 tatagtcaaa atctcgaaga acctatgttt caaactgcta atctgatcac ggaaacacaa
    31261 tatcaagcct tcagaaaagt taatccagat ttgccgaaca tttctttgaa atcacctggt
    31321 catactgttt tgcttgatca aatgcaaact ctttttcgga atatttcatt atatggatct
    31381 agtattggac tgccagataa tcaagaattg aagattcaac aaatgcttcc aagtgtatta
    31441 ggtgacgaaa caatggtata tctaagtcct gtactgattg tagatgatac cgtttttgaa
    31501 aaagcaaaag gtgtggatta tcaagtagtc aatgtagatg tatcaggtgg aaataatgaa
    31561 aaaatagatg aacgactgag caatgacctg tcgataaagt ggctcaatgc tgtttattat
    31621 tcttatgata tcgtaaatgg aagtctacaa ggagaaatta gcacaaagaa acttccggat
    31681 gaccaaatga aggaagcaca atttagccaa gaaagctctc gtttgaattt ctcttccagc
    31741 tattcgaaat tgcgtgcagt taatcggcga atcggtatca atatttttgt cacactattt
    31801 gtcgggatga tcgtgattat tgctactggt agtattttga cagtccggca attagcagaa
    31861 gcagaggaag aaaaagaaaa ttatcaccta ttgactaagc tggggattcc acaaagaagg
    31921 atcagacgga tgatcttcga gcaaaacgca cttaccttct ttccacccat gatattaggg
    31981 atcactcacg cagtatttgc aatcaatgtc ttcactcagt atataaaaac tgccgattac
    32041 tggttggctt atcttgtgag tggcttattg atccttatct atcttttgct ttatgtcatt
    32101 acgagtcagt tatattgtcg gattatcgag gaatgatata cggaataaga caagataata
    32161 aatagaacta aattttgaat accatttatt tggaataaga ggttgtgaca cgatttttgt
    32221 cacaacctct ttatttttta tattttttaa aagtacgaga aattataaag aaaaaagcag
    32281 tgatttgatg tgctattttt ataaaagcta tgtgacaaaa ctgttagtaa aaaaacggtt
    32341 tctgtaagct agacacatgt ttctttttgt caaaattagt tacactttgt gtgaggttat
    32401 atgaggaggg aatccaatga acaaatggcg ttctaatcca aaattgaatt attggggaaa
    32461 attcatcatt cgaacaattt tttatagtgc agtactgctg ttcctgattt atctgtatca
    32521 ctataaaaat attcaaggag gaacttttat ctataacgaa ttttaggagg aagagtatgg
    32581 aaatcaaaac gattattgaa gcaatcgatg attggggaat aaatgaacct gatcgagttg
    32641 cttatcaggc ggatgagacg catacttttg gtgaattgaa acaagcatca gacgcacttg
    32701 cttattattt agaaaaaaag gtagaaggcg atggaccaat tgtcgttttt ggcaatttgg
    32761 agtttgaaat gattgtctca tttctagggg ttgtcaaatc tggccatgcc tatttgccga
    32821 ttgaagagca tacacccaag gagcgtatcc tatcgatctt tcgggtggct aaaccatcta
    32881 tggtcatcag tatcggggac tggatagaag atattccttc ggttcctgtc ataacaaaaa
    32941 aagaatttca agaaatcaag aagataccag taggatttta tcccagaaat agcgtaaaag
    33001 gtacagaaaa tttttatatt atttttactt ctggtactac tggagaacca aaaggcgtac
    33061 aaatcagtca tgataatcta gtcagctttg ttcaatggac gatggaagac tttgggatca
    33121 agcctggtat gcatttctta gcccaagcgc cattttcttt tgatttatcg gtattttcta
    33181 tttatcctgc attagtttct ggcggaatgc tgaagccgtt aaataaagca gttgttcaag
    33241 actttcgtca attattcgcg acattgccag ccttgaagtt gaatgtgtgg gtttctactc
    33301 catcatttat ggatatttgc ttgatggaac ctacattcaa tggagaaaat gtgccggaat
    33361 tggacatgtt tttattttgc ggagaagaat tgacgaaaaa aactgcagaa agtctacttg
    33421 atcgattccc aaatgcacgg atatataata cttacggtcc aacagaagca actgtagcta
    33481 tttcaagtat tcagattgac cagcatgtac tagatagtta tgaccgacta ccgattggct
    33541 acgtaaaaaa agacacgaaa gtttctattg tccaagaagg taaacctgtt tcaaaaggag
    33601 aaacgggtga aatcattatt gcaggaccaa gcgtatcaaa aggttacctg aacaatcccg
    33661 aaaagacagc tgctgcgttc ttttgtcaaa atgacttagc aagttaccat acaggggatg
    33721 caggtagact agatgaatat gggcttttat tttatgaagg acgtatggat cagcaggtca
    33781 aattacatgg ataccggatt gaactcgggg atatcgaaca ttatctgctc caagacaacc
    33841 gaatcaaaca agctgtcgtc gtaccaaaat accaaggaac aaaagtacag cagctggttg
    33901 cttttgtcgt attagaaaac aaaacgaaac agccggattt tcagttgact aaatctatca
    33961 aagaacaatt actagaagtt gtaatggatt atatgattcc tcagaagttc cattatgtcg
    34021 atctgctgcc tcagacagtt aatggaaaaa tcgatcgaaa aaaattaaca gctgaggtga
    34081 atcctgaatg atgttacctc atatgatccc ttatgcagag ccgttttatt tttttctttt
    34141 agccattgcg cttgtaccga tcgttctatc tttattgata tgggaaaaaa gattaccagt
    34201 ttatcaatca gcagtgacac ttttcttttt atttgtcagc tttggaggta attattggca
    34261 acagggagcg gcgctgattg ggtatgtttt gtggcagacg atacttgtat ggggctattt
    34321 tgtttatcgg caaaggaaaa atcagacagg atggtttttt gccgccgtta ttttagcgat
    34381 aattccactg tttttaacga aagtgactcc tttttttgaa catggaaaag catcgttact
    34441 tggtttttta ggaatctctt atttaacctt taaatctgta caaatgatca tggaaataag
    34501 agatggcatg atcaaaacat ataagccatt tcgttatatc cagtttctgc tgttctttcc
    34561 aacaatctct tctggaccaa tcgatcgcta taggagattt gaaaaagatc agctccatcc
    34621 gcctgttcgt gataaatatg ttgatctttt gggaaaaggc gtccaaaata tttttgtcgg
    34681 ttttttatat aaattcatta tcggttatta ttttggtcaa gtactgcttc ctgtagtggg
    34741 acgcattgcc ttacagcatg gtggtatttc atgggcatta gtaggttata tgtacgtgta
    34801 cagtatgtat ttgttttttg attttgctgg ctatagctta tttgcagtcg gtacaagcta
    34861 catgatggga tatcagacac caatcaattt caataaacct tttttatcat ggaatatcaa
    34921 agaattctgg aatcgttggc atatgacgct gtccttttgg ttccgtgatt atgtatatat
    34981 gcgattgatg ttcttcatga tgaagaaaaa attttttaaa agcaagatcg taatgtcaaa
    35041 tatcggttat tttgctttgt ttcttctaat gggtatatgg catggattga cgtggtacta
    35101 catcgtatat ggactgtatc atgcttgtct gatctgtctg acagatgcat ggatccgatt
    35161 taagaagaag cataaagagc agttgccttc taacaaattt acgcatggct tagccgtatt
    35221 tttgacattt caagcagtat gttttagctt cctgattttt tcaggatttt tagataaact
    35281 tttttttagc taacgaatag tgacatatta atttatataa caaaggagaa aaggaaaatg
    35341 gaagaacaaa ttttatcaat cttagaagaa ataacaggaa cagatgaagt aagaaaaaat
    35401 cgtgatattg atctatttga tgaagggttg cttgattctt tagcatctgt tcaattatta
    35461 gtcgagctag acggagagct ggatatccaa gtccctgtat ctgaattcga gcgagaggat
    35521 tggagcacac cgaataaaat cattcaacaa gcaactgcat tgaagggttg agtatgaaaa
    35581 agaaactttt tgctattttc ggcccggttg ttttagctgt tggcttactg tttgtgtttt
    35641 ttacctcctc ctatcaagta gatatctcta atccagaaac gattagacgt gcctcttctt
    35701 ccatgtcagt gaacgtgttg aaaggagatg cgataaaaaa ttatgcatta tctgaaaaac
    35761 aatatattcc tttttttggc tcaagtgaat taagcagaat cagtccattt catccgtctg
    35821 ttctggcaga aaaataccag cgaaactatc gtccattttt gctgggagca cccgggacac
    35881 aatcgttaag ccaatatatg atgatgcgtt ctgcaggaga tgctatgaaa aacaagaaag
    35941 tcgtctttat tatttctcct cagtggttcg ttaaaaatgg tgtaaaaact gattacttta
    36001 atacgtatta ttctgaactt caaacatacg actggttgtt ttcaatgaag aaagtaacgc
    36061 ctgctgatcg ctatttagct agaagattat taactttctc aaaagtaaaa gagaatgata
    36121 cgttgacggc gattctgcaa acgatcaaaa aagggaagct acctttacct gaatctttga
    36181 atcagctgcg cagccaatgg aacatgctga agagagagga tgaagtattc agcaatattg
    36241 ggttgaaaga tcgacagcaa aaaatcgatc atgagtcaaa acgattacca aaacaatacc
    36301 aagaaacaga attatccatt ttagcaaatc aaattggaga acgagaaacg acgaataatc
    36361 cattcgggtt gaaaaacgat ttttatacgc atcgtatccg agcccacgag ccagagctca
    36421 aacagtcaca aaaaaattgg gattatcgtt tttcgccaga attttctgat tttcagctgg
    36481 tcttggatca attagcaaaa aaccataacg aagtattgtt catcatccca ccggttaacg
    36541 agaaatggag cgattatacc ggactatctc aagaaatgtt acaaggattt gccaaaaaga
    36601 tcaaatttca attaaatagc caaggattca atcgaatagc tgattttgtc aatcaagcgg
    36661 gtaccaacta ttttatggaa gacacgatcc atctaggctg gaaaggctgg ttggctgcag
    36721 atcaacagat cagaccgttt ttagaagaaa atcatattac tgctagtaag tatcatcttg
    36781 acgatgcttt ttttagcaaa tcttggcaac atcaaatacc agataaacta caattaaaat
    36841 aaagaaaaca ggcgagtgtt gctcgcttgt tttctttatt tttgaatatt ctgaataaaa
    36901 gataaaaata ccaagtttgc ctgaacaaaa atcataagca atggtataat cacaatatga
    36961 atatagaaat gggggaaaac atttggatac aaaagaagaa cagctactta ttgatttaac
    37021 ttctgcaaaa ggagtacctg gaaatgaaga agaggtaaga gaagtattca gagaatatgc
    37081 caaaccattc gctgatgata ttttttatga cggtttaggc agtgtgattg ccaaacatgg
    37141 tgctggcgga ggccccaagg tctttatttc tggacatatg gacgaagttg gttttatggt
    37201 aacaaaaata accgaaaaag gattcttgga attccaaact cttggaggtt ggtgggggca
    37261 agtcatgctt gctcagcaag ttgaaatcaa aacacgtgaa gggaaaatcg tccacggtgt
    37321 gattggttcc aaaccaccac atgttttaac acctcaagta agaaataaac cttatgaaat
    37381 aaaggatatg ttcattgata ttggcgcatc aagccaagaa gaagccaaag agtggggcat
    37441 acgtcctgga gatatggtca ctccatatat tgaatacaaa cgaatgaatg gcagtaaata
    37501 tttactggca aaagcatggg ataatcgtat cgggacagct gtttctctgc gagtattaga
    37561 gaatctatct aaagaagcac acccaaatgt tttattcgca ggaagcgatg tgcaagaaga
    37621 agtaggcttg cgaggtgctc gaacaagtac acaccttgta aatcccgata ttgcttttgc
    37681 tttagatact ggtacagcag gagacactcc aggaatgaca ccaaaagaag cagattcgat
    37741 tttaggaaaa ggcccacaga ttttgatttt tgatgcatct atgatcccgc ataaaaaatt
    37801 attgaatttt gttattgatg tagcagagga aatggatatt ccattccaat atacagtgat
    37861 ttctggcgga ggaactgatg caggacaaat gcatttgaca agagatggtg ttccgtctgt
    37921 agccattacc gttcctgttc gttatctgca ttcccataca tcagtgattc atgaagatga
    37981 ttatttgaat acagtgaaac tagtcacaga attagtgaaa cggcttgata gtgaaacagt
    38041 tgaaaaaatt cggagttatg aataatgaat aatagaatac ctatttctga atatcggaaa
    38101 aaagcacgag tttctttgga aaatcaatgg ggaatcaata cttggcttgt tttcttatct
    38161 ataatattta cgagcgtcat ccagaacttt tttgacggtt tcagtccttt tccggaagaa
    38221 agcatgcagc agcacttatt gagttttcta gtcgataatt tacttttatt cgcattcaac
    38281 tatgcattgt tttacattgc gctagtagtt gtacggggtg gcagaccaaa aagtaacctt
    38341 ttattttcag ttttccaaag aaagtatttt ggttcgatac ttggcatcaa tttgatcaat
    38401 tcagtcatca actggctact caacagttta atctttctgc ccatgttttt aatcagcgga
    38461 gtgagtactt atatgcagct gcttttcagt tcaaatgcgg tacctacggg tgtcatgcca
    38521 gaagattttc ttgatatcag ctttgtttta tcgatgatag ggatgatttt tgtttcagca
    38581 ctcatcatga gtattgttgg cggtttattc caatttgctg catggacaaa gttcgactat
    38641 cctgatttgt cggttattca atgtttgaaa tatggctggt ttttattgaa agatagaatt
    38701 ggtacgtata ttttgttgca gctgtctttt atcggctggt atttgctagg agcaatcgct
    38761 ttgttcatcg gtcttttgtg ggtcattgtt tatgtgaatg tgtcagttgc agaattttat
    38821 gaacaagcac ggattgaaaa aggaggtcca gacgcctatt ttacttagca tgtaagcagt
    38881 ttattcatca aaaaaataac ttttgtggta ggataaatct ttgaggggga atgtacgatg
    38941 aaattaactg ttttaggatg cttaggagcg tatccttata aaaatcaagg gacaacaggt
    39001 tatttgcttc aatctgacgg gttcaatttg ttgatcgatg caggtagtgc tacgctgatc
    39061 aaattacaag aacaccttga tccacttgat ctagatgctg tgatccttag ccattatcat
    39121 catgatcata ttgctgacct tggtgtatta cagtattact ggcaacttca tcctaagaga
    39181 aatccggaga cattgctttc aatttatgga cacacaaaag attccattca ctttgaagaa
    39241 ttgacgttgg aaaatgtctc aaaaggacat ccttattttg aagctgaaga attaaaaatt
    39301 ggtccttttc tgattacttt tatggaaaca atccatccag tagtatgtta cgccatgcgt
    39361 atcgtagaag aaacaacggg gaaagtattc gtatttacag gtgattcagg ttatttggaa
    39421 agtttcgctg attttgcgaa agatgctgat ttgtttctag cagataccta tctttttgaa
    39481 ggaaatgaac atcatcatgc ccatttcact tcaaaagaag ctggagaact tgcgaaatca
    39541 gctaacgtaa agaaacttgt acttacacac ctacctcagt ttggtgattt agaacaattg
    39601 aaaaatgaag cgcaaaaggc agcaggcaaa gggattgata tagaacttgc tgcagttgac
    39661 aaagtgttta acatttagga ggaagaacat gatttttgta ccaaatgaaa accacgaccc
    39721 aagaatcaat ttagcaattg aacaatattt gctgcaagaa atgcctttag atgaacctat
    39781 tttgctcttt tatatcaatg aaccttcaat catcatcggc agaaaccaaa atacgcttga
    39841 agaaatcaat cgagattatg tggaagaaaa tggcattcat gttgtccgtc gcttaagtgg
    39901 aggcggagct gtctaccatg attttggaaa cctgaacttt agttttatca tgcctgatga
    39961 tggggattca ttccgtgact ttgcgaaagt aacacagcca atcatccaag cacttcatga
    40021 attgggtgtt gctggggcgg aattgaaagg ccgaaatgat ctagtcattg atggcatgaa
    40081 attttctggc aacgcgatgt atgctaccaa cggtcggatg tttgctcatg gaacaattat
    40141 gttcgactct gatatcaatg aagtagtcaa tgcattaaaa gtaaaaaaag acaaaatcga
    40201 atcaaaagga atcaaatcga tccgttctcg tgtaacgaat atcaaaccat ttcttccaga
    40261 agaaaagcaa aaaatgacaa cagaagaatt tcgtcaagaa atcttgttga aaattttcgg
    40321 cgtagagtca gtagaagaag taaaaacgta cgaattgact gatgaagatt ggaaaaaaat
    40381 caatcgaatc tctgaggaat attatcgaaa ttgggattgg aattacggga agtctccaga
    40441 tttcaatttc tctcgccaaa aacgtttttc tatcggttcg attgaagtcc atttgaatgt
    40501 ctcagaagga atgatcaaag aagcaaaaat ctttggcgat ttcttcggac taggagatat
    40561 ctctgacgtg gaaaaaaaat tggttggaca gaaatatgat aaagcatctc tatcaaaaat
    40621 agtcagccaa attgatgtga agaaatattt tggtgcgata gaaccagaag aattgcttac
    40681 cttactttat tgaaaataaa aaatagcgga atccacatat atggattccg ctatttttta
    40741 tacaggattc gggtaatttc gagagagttg aacagtgatc cgatcaatga aagtatcttt
    40801 ttcctgcgtt agtgttcctg tgtaacgatc cgctacgtct ttcatcatca ttaagtcaaa
    40861 acgttcctga tcatctaatt cttctgtgat gcctcgacta aataggtttg tatcgtaatg
    40921 tgcactatgt tcgcaacaaa tgacgaaatg ttcctcttgt tcgtgtatcg acaaataaat
    40981 ttttcgatac agtggatttt cgatggcatg agtttctcta aaggaatgct ctaaagcgtg
    41041 gatcaaaagt tgagccagat cgtcatcgtt gataggaagt tcttcgggaa gatctgcttg
    41101 gaaggttatc tgaatattcc gtttggcagc taacttctgg taacgcgcta agataagatt
    41161 tgtcacctgg tggttgcaaa aagaacacag catttcgaaa ttttttgctt cttctaatat
    41221 cttttccaca tagtttttag cttttgtttg gtccgtatgc attaaatctt gcagattctc
    41281 catctcttgg acaaattcca ggcgaagtgt atttacttct tggatatgct gtcgaagctg
    41341 ctcataatgt tgctccaaca aatcattttt gatttttaag aaatctactt gtctccgatc
    41401 tttgtcggta tttaagatgt gatcgattag attgtagctg ataataacga ttagaatgat
    41461 aatatataaa atcgtagacc aattgatcgt tgtttgatag atacctaatg ccgtttggat
    41521 gtaaatcaag caatggataa tggctaccaa gacgatccaa ggtgtacaag ctacataaaa
    41581 acggttattc ttatatgctt cgccaatgca tgcaagtgaa gtgactaaag tgcttataac
    41641 actagcaata ttagcaatcg gcattaattc aggaagttct gcaggagtaa aagttgcata
    41701 aagcagaacg ccgataaaaa tcaaaagttg gaacgtacaa aaaattccgt accatttttt
    41761 atagtaaaac attcggctcc aatagtagca gatgatgaaa gctgacgtta agataataaa
    41821 taaatgagat aaaaaagaat gaacgaatgg ttcaaaaaga ataccactta aaatatcttc
    41881 tgatacagct tcaaatccta atgccaaagc aaagcaactt aagagaataa gtgagtattc
    41941 aagacgtttt tgtttataca tcaagtagat cgtatagaaa aaggtcccta tagcgataaa
    42001 aacagcgatt atcatcgtca atacttgcgg cactgattgt gaataaataa aactaatcaa
    42061 tggtccagtt gttcctagat aaactttcgg tggcagtcca gcatagttct gataaggaga
    42121 tgagactgta agggtcaatt tttttccgat ataatttgct ggtaaatcta tcacagccaa
    42181 tgagagtccg ggattgcttt cttcctgata atctttccgt tggtataaaa tcttttcgcc
    42241 tagatataca gtaatccatt gatggtttcc tttgattagt aaatttgctt cgtccaacgg
    42301 tttttcaagt gtcgtttcca taatgaatgt ttcgtcttta tccacggtag gaatcgtacg
    42361 agtgtacttg ctgtcaaact taaaatcagg atcatctttt gtatagaaaa cccacgaatc
    42421 ttttatttct ttcatttgat tgctgtccgc aggagaaaaa ataaaataaa agaaataagc
    42481 aattcct
//
LOCUS       DASJPC010000016        37796 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJPC010000016 DASJPC010000000
VERSION     DASJPC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.016"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            325..1374
                     /locus_tag="U4A13_000821"
     CDS             325..1374
                     /locus_tag="U4A13_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1779141.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            1371..2252
                     /locus_tag="U4A13_000822"
     CDS             1371..2252
                     /locus_tag="U4A13_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779142.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            2255..3025
                     /locus_tag="U4A13_000823"
     CDS             2255..3025
                     /locus_tag="U4A13_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1779143.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            3304..4158
                     /gene="aroE"
                     /locus_tag="U4A13_000824"
     CDS             3304..4158
                     /gene="aroE"
                     /locus_tag="U4A13_000824"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN1779144.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            4336..5499
                     /locus_tag="U4A13_000825"
     CDS             4336..5499
                     /locus_tag="U4A13_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1779145.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            5616..6026
                     /locus_tag="U4A13_000826"
     CDS             5616..6026
                     /locus_tag="U4A13_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1779146.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6200..8428
                     /gene="topB"
                     /locus_tag="U4A13_000827"
     CDS             6200..8428
                     /gene="topB"
                     /locus_tag="U4A13_000827"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1779147.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            8599..8883
                     /locus_tag="U4A13_000828"
     CDS             8599..8883
                     /locus_tag="U4A13_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779148.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9043..9279
                     /locus_tag="U4A13_000829"
     CDS             9043..9279
                     /locus_tag="U4A13_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1779149.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            9276..9674
                     /locus_tag="U4A13_000830"
     CDS             9276..9674
                     /locus_tag="U4A13_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1779150.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(10341..11285)
                     /locus_tag="U4A13_000831"
     CDS             complement(10341..11285)
                     /locus_tag="U4A13_000831"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN1779151.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(11581..12525)
                     /locus_tag="U4A13_000832"
     CDS             complement(11581..12525)
                     /locus_tag="U4A13_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779152.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            12746..13960
                     /locus_tag="U4A13_000833"
     CDS             12746..13960
                     /locus_tag="U4A13_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1779153.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            13982..15997
                     /locus_tag="U4A13_000834"
     CDS             13982..15997
                     /locus_tag="U4A13_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN1779154.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16080..17453)
                     /locus_tag="U4A13_000835"
     CDS             complement(16080..17453)
                     /locus_tag="U4A13_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1779155.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(17479..17763)
                     /locus_tag="U4A13_000836"
     CDS             complement(17479..17763)
                     /locus_tag="U4A13_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1779156.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(17765..19780)
                     /locus_tag="U4A13_000837"
     CDS             complement(17765..19780)
                     /locus_tag="U4A13_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1779157.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(19959..20261)
                     /locus_tag="U4A13_000838"
     CDS             complement(19959..20261)
                     /locus_tag="U4A13_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN1779158.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(20350..21381)
                     /locus_tag="U4A13_000839"
     CDS             complement(20350..21381)
                     /locus_tag="U4A13_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1779159.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            21588..22463
                     /locus_tag="U4A13_000840"
     CDS             21588..22463
                     /locus_tag="U4A13_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1779160.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(22615..23457)
                     /locus_tag="U4A13_000841"
     CDS             complement(22615..23457)
                     /locus_tag="U4A13_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1779161.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(23549..25426)
                     /locus_tag="U4A13_000842"
     CDS             complement(23549..25426)
                     /locus_tag="U4A13_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1779162.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(25423..26844)
                     /locus_tag="U4A13_000843"
     CDS             complement(25423..26844)
                     /locus_tag="U4A13_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1779163.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(26847..30236)
                     /locus_tag="U4A13_000844"
     CDS             complement(26847..30236)
                     /locus_tag="U4A13_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN1779164.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            30493..31884
                     /locus_tag="U4A13_000845"
     CDS             30493..31884
                     /locus_tag="U4A13_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779165.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(31957..32604)
                     /locus_tag="U4A13_000846"
     CDS             complement(31957..32604)
                     /locus_tag="U4A13_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN1779166.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(32612..33298)
                     /locus_tag="U4A13_000847"
     CDS             complement(32612..33298)
                     /locus_tag="U4A13_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779167.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(33304..34377)
                     /locus_tag="U4A13_000848"
     CDS             complement(33304..34377)
                     /locus_tag="U4A13_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779168.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            34599..35273
                     /locus_tag="U4A13_000849"
     CDS             34599..35273
                     /locus_tag="U4A13_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1779169.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            35278..36618
                     /locus_tag="U4A13_000850"
     CDS             35278..36618
                     /locus_tag="U4A13_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1779170.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(36767..37753)
                     /locus_tag="U4A13_000851"
     CDS             complement(36767..37753)
                     /locus_tag="U4A13_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN1779171.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT        11586 a         6925 c         7151 g        12134 t
ORIGIN      
        1 gattagactt aaatcaattc ttaaggaaag ggtgacctgt aatgaactta tcaaaacgta
       61 tttcagcaga aaacaaaaaa aattggcgca gctggcgtag ataagttgta ttaatggtga
      121 gactttccca tagatacaac tttttagagt gcatttctaa agcttgtatc tatgctagac
      181 atgaacattg actgccgcat agattagaat ctatgcggtt ttttgtgtaa agacaatgac
      241 cgctttggcc attgtctttt tttgtagact ggatacttgc ctgattataa taaataaaaa
      301 gattgacttt gcaggaggaa aaaaatgaaa aacaaacgat taataacagt tgtagtaatt
      361 attttagttt atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt
      421 acttcgacga accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat
      481 ccggcattag atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa
      541 gaaggagaaa atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc
      601 acaatgagtc aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct
      661 gccgctcaat cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg
      721 gatcctgtgg gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt
      781 gtttctgatc agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat
      841 gcaaaaaaag tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca
      901 caagcagaag aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc
      961 actaatgaga ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt
     1021 ccattagata ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca
     1081 aatatccccg tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc
     1141 gggatcaatc aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt
     1201 ggaaaagaaa aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc
     1261 aatcaatctc aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc
     1321 aaaatcatta cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat
     1381 cagctatttc acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc
     1441 ggatcttaga ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct
     1501 gtgtgacagc tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg
     1561 ctggcatgat agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta
     1621 ttttagcagg gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa
     1681 caccaaatct ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt
     1741 tacctgatta ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg
     1801 tactgctatt cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg
     1861 aaacgatggc tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt
     1921 tgtcgaacgg tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag
     1981 atgtaaataa agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag
     2041 tgatcttcca agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca
     2101 tttatcagct attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt
     2161 tgttctccgc tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga
     2221 acttaaaaac gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa
     2281 tggcagaaaa gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa
     2341 ccttacaatc agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag
     2401 cacactgttc aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa
     2461 agatcagctg atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt
     2521 ccaagacccc aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc
     2581 acaaaaaaga ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga
     2641 ttttttcaga ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc
     2701 gggtgaatta tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc
     2761 tcctgagtta ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc
     2821 attaatgcga ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac
     2881 gcatcgaatg gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa
     2941 aatcgttcaa gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca
     3001 gttctttgaa gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt
     3061 gaaaactcat tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac
     3121 atatttgggt gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc
     3181 tttgctctga caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg
     3241 tactttttcg tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat
     3301 aagatgattt ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt
     3361 ttatccccat tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta
     3421 gctttccaag taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat
     3481 atgttaggtg taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag
     3541 gtaagccagg aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt
     3601 ttgattggct ataataccga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt
     3661 tcgatcaaaa agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact
     3721 gttcaagctg cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca
     3781 ttcgctcaag taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa
     3841 gttcgtgact ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta
     3901 gtcaatgcaa cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata
     3961 agcatgatgc atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt
     4021 ttattaaaag aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc
     4081 taccaaggag caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt
     4141 gaaaagctgt ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg
     4201 tctcaagaaa agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt
     4261 atcaaaaaat gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa
     4321 cgaggaggaa ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa
     4381 cctctgaagc gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg
     4441 gaaaatgagg aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg
     4501 aaccaatacg agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat
     4561 aaaaaatttt tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg
     4621 tggaaaaaaa tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt
     4681 tttattggta aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc
     4741 tttcatgggc aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca
     4801 cgcggagaag atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc
     4861 cataagccga aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga
     4921 caaaataaga tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta
     4981 gtagaaaatt ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa
     5041 aaagtgatcg atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat
     5101 ctggatggtg tagatattaa aaaaggggtc cagaagatgg atggacatgt tcttttgcag
     5161 tacgcacgtt tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa
     5221 gtaatgaacg ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat
     5281 gcagctggta aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa
     5341 aacctctttt cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac
     5401 ggttcgtgga attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa
     5461 atgaatcaat ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga
     5521 agctgcgcaa ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct
     5581 tatggtagat ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa
     5641 atgagtcgcg tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta
     5701 tcaccacata cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa
     5761 atctaatcac gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc
     5821 cagaaggaat tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa
     5881 gaaatgaaga agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga
     5941 tgccactgga tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca
     6001 tcaactggca gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag
     6061 cagcaaacgg atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta
     6121 tctttttttt attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt
     6181 aagtaacgga ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa
     6241 agcctatgcc gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga
     6301 tccattgttt gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt
     6361 tgctccagga gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt
     6421 cccagaaacc ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa
     6481 acgagaattg cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga
     6541 agccatcgcg tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt
     6601 acgtttatgg atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca
     6661 acctggaaag aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg
     6721 gctgattgga atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga
     6781 tggcagtttt tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca
     6841 agaagaaatc aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac
     6901 aaacaaaggg cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga
     6961 gttaacgaag gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct
     7021 tgaagttacg aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt
     7081 gcagacaaag atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca
     7141 aggattgtac gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga
     7201 aaatgagcat cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga
     7261 agaagtacag acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca
     7321 agaacatcat gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct
     7381 ttccaaatta cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc
     7441 tgcagattat atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa
     7501 aagcatcgga aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa
     7561 aaagaaagaa gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact
     7621 gaaaagtatg gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac
     7681 agcaatgaaa actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga
     7741 agtagaagga atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa
     7801 gcattacatc gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg
     7861 taaggcagta gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg
     7921 gtacttgaga aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa
     7981 aaaattcatc ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc
     8041 agatgagatt agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg
     8101 aagatgtcca aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt
     8161 gaatccggaa tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac
     8221 gaatctcaga gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg
     8281 taaaaaaggg aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc
     8341 ttttccatgg atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag
     8401 aaaaaataat gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc
     8461 cgaaaagtga gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac
     8521 gttggggtag gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat
     8581 tcatttttaa attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta
     8641 aagtatcctg attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca
     8701 ccaacgttga ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat
     8761 aaaacgacaa gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg
     8821 gctatgatca ttatttattt tctaccagtt attacacaag tatttcatca atattttggt
     8881 taagaatacg agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt
     8941 cgtaagaaaa agcaaaataa aagtatatac attgattaaa agtatatact tggatataat
     9001 gtacttacta tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag
     9061 tataaaatta gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa
     9121 ttcatgggtg tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta
     9181 cgaatgatca aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat
     9241 caatatgaag acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa
     9301 ttatcaaaat caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa
     9361 aagatgcaaa tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag
     9421 aactctatcc tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac
     9481 atccttttca taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat
     9541 tgaataattg tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta
     9601 ccactagttc attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa
     9661 ccgcaataaa atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag
     9721 gaatatgctg atttttttat gtttaatcat acaaaatagt tttttcataa aaatatggaa
     9781 aatatggatt aattttgaaa taattgattt aaaaggaata caaaagagtg aatagtagga
     9841 aatagtggtt tagaactagt gggctgattt tttatatatt ttagtttaca taatgtatat
     9901 tatacaaagt agatttaaaa aatgataaac ctctctcgaa cggtttttta tcaaaaatcg
     9961 tcatttttcc atcaaaatct gcttatgcaa aattataaaa aaattttgca gtttttctta
    10021 ctttttttgt cgtttaattg aatgataaaa accagtttct ttattcagtt aatcaaatat
    10081 gaacgaatta gatatgaatc attgtatttg ccgttgcgga aggatttttt aattaaccac
    10141 agataaacat tgataacgat tcgcttattg taatcaacag attaaatttc atatgttcat
    10201 atgtacggat acattttcgg accgattaat tttaaccatt ctgtttattt gcttacctat
    10261 tagatatcaa taaaaaactg cttaacagct atttcagctg ttaagcagtt ttaggatttt
    10321 tagttcatat tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt
    10381 gatttgctga acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca
    10441 ccattttcgt caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca
    10501 gttaaataag cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata
    10561 cgtgcagtac tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa
    10621 gcagcattac ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct
    10681 ttttctacaa tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact
    10741 tctgaatcac catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat
    10801 ttcaatgcga tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca
    10861 cgtgatgttg gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt
    10921 gaagcaacaa cgataatacc gtcgaatcct gatcccatga tttcttttac gatttggcgc
    10981 atgattgacg catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata
    11041 ccagctgtga taacaacgat atcagcatct ttacattctt catagccacc tgaccataca
    11101 gccacatttt catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct
    11161 ttttcttgat tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca
    11221 taagcgatac ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt
    11281 ttcatgaata aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt
    11341 gtttattcat tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa
    11401 cgaattcctg aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata
    11461 atacaaccaa aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat
    11521 aagacagcaa tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc
    11581 ttaatgttta tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa
    11641 taaagaagtc gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt
    11701 ttcttgaatc agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga
    11761 agcatcaaaa tacattttca atttattact aagatagacc ggattgtagc caaaatgagc
    11821 tgctgtttct tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag
    11881 catctcattt tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt
    11941 caattcgatc agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt
    12001 atcaaaataa atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc
    12061 gcgaaaatga atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga
    12121 agaaagtaga ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc
    12181 ttttaataag atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgagtagc
    12241 ttctttatca atgatcacca cgtcactttc ttccatctct atcctttctc cattaataat
    12301 ttgtaaacat gtaccgctat acatataatt gatttcaaca aaatcatgtg tatgctcggg
    12361 aacttccgca tatcgacggt gtttactgat atgaatattt ccctgttgaa atagatcccc
    12421 tcggtggatt tttggcggtg tctgaagatg gtaagctaag tttaaatcat tgatattcat
    12481 tccttgttca cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt
    12541 cagctagttc caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac
    12601 gctgtactgc tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg
    12661 acatgaactt cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat
    12721 aagcgcttac aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct
    12781 gctgtcagtc tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat
    12841 gtcagctact tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc
    12901 ttttatggtc actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa
    12961 acaaactgtg atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc
    13021 acaaagttac agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat
    13081 caattctttg gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat
    13141 gattggtttt caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg
    13201 acagctagta cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat
    13261 tcttgtcttg tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg
    13321 cacagcccca acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt
    13381 tctcttgctg gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac
    13441 gacttattct ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc
    13501 aagtatggct gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct
    13561 tacactttct ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct
    13621 gtatgtgatt gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta
    13681 cttattcaac caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat
    13741 cttattggtt ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc
    13801 ttttgcttct cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt
    13861 gattagcgcg cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag
    13921 agtagatgaa gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa
    13981 aatgacaaat gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg
    14041 ccatggactg ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg
    14101 gtacatggat gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc
    14161 ttccatgttt aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca
    14221 tccatgtgga aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg
    14281 acatacatca gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg
    14341 ctggacagca agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga
    14401 tatcgcaaaa gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga
    14461 aacggagatt ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga
    14521 actaatatct ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt
    14581 agaagctatg gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa
    14641 agctcgtaaa cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca
    14701 acttcaagtt tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc
    14761 ttctttactg aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt
    14821 gttttttatc gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc
    14881 attcatgatc aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc
    14941 attaagagga caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt
    15001 gtggttgatt ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat
    15061 cgtctaccct aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg
    15121 gtttatttat gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga
    15181 aggaacgctt gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg
    15241 cggagatatc ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca
    15301 agaaaaatat caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg
    15361 cgcttttatc gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt
    15421 cgctgtttta ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt
    15481 attgaatgac actatcacac agattactac tccatatttc aaattctatg aacaagatgc
    15541 tttatgcaaa ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg
    15601 gatgttatct catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa
    15661 tgaaaagtac agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc
    15721 aagcccaatc tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta
    15781 tgaagaattt atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc
    15841 tatcaaagat ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga
    15901 tagaacaggc ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc
    15961 acttgtctta acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata
    16021 tcagaagact ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct
    16081 taattctgtt ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat
    16141 acgacaagta aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct
    16201 agtttcccaa gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg
    16261 attttaagat ctcctaatac tggaaggata aataatggca gccaactaat caataagcta
    16321 ttgaaagcag aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca
    16381 gaagcagctc cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag
    16441 cctatcatag caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat
    16501 acagcattag gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt
    16561 acgagttttt tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc
    16621 ccttgaagga cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt
    16681 acagcgttgt ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca
    16741 agtgcgacat atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag
    16801 ctgaaacttt taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt
    16861 ccgatccatc cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg
    16921 tttccagtga tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt
    16981 ccttcgaaga ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact
    17041 aaaatgactg ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat
    17101 ttatatcttg tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa
    17161 gcagtaggtg taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact
    17221 actccttgag tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg
    17281 attaaaccag caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg
    17341 atcacttctg aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg
    17401 aggatggagg gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt
    17461 tcctcccttg aatcattttt attcttttga caagatcttc gttacttttt cttttacttc
    17521 ttcgctgtca atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt
    17581 atctttcaaa gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac
    17641 atccgtatga cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt
    17701 gatgttcatc tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt
    17761 catatcattc gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta
    17821 ataaatcttg aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa
    17881 gatgtgcctc attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat
    17941 tatcaagcag atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc
    18001 catcttctgg acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat
    18061 actgctccac gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata
    18121 atagcggctg tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt
    18181 ggatcatttc ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta
    18241 aaagtttgtt cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt
    18301 ttaattcgat ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc
    18361 ctggtatcag ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca
    18421 tgattggatg cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag
    18481 aaaaaataat atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct
    18541 ctgtaaaaaa gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc
    18601 taatgccact tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg
    18661 cttttctgat ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc
    18721 tcttccctat tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag
    18781 catattgatg cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac
    18841 gaaaataggc agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa
    18901 attctacggc catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa
    18961 aatcaagtgc aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa
    19021 atataatctc cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa
    19081 tgtcagtgac tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt
    19141 ttccaattcg aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg
    19201 cgaatcgaag atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat
    19261 aatcaatgga aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca
    19321 tcaaacgagc agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc
    19381 taaaaccagt tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta
    19441 tctttttgat atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa
    19501 aaacagaaac agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt
    19561 gttcggtata cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg
    19621 ctcggaattt ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt
    19681 gtttgatcgc ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt
    19741 ctcttggtgt tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact
    19801 tatattctag tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga
    19861 ttttttttac gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga
    19921 ccaaatgtct cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt
    19981 tttgaggtca gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt
    20041 tcaaagtgat tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa
    20101 gctgtttctt tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct
    20161 tctgtttcac cagaattagt tcctgctaaa gccacttctt ctgttttagg ggccattgaa
    20221 cctcttaatc gtttgtgttt ttcaccgtca taatttgtca tgttggttcc tcctagtagt
    20281 tctatacttt ttcatcgtac aataagacag tttatgcgtc aaaaaacaaa cccgattctt
    20341 acaaatatgt catatttttc cttctgattc ttgaatgctt ttgattcctt cttccaaaat
    20401 tttcatttgt aagattgttt cttcatcttt gatgactttt tctgcgccat taactaatgt
    20461 ctcgtaaatc ccttcgtaga cacggctgta atcaccaacc tcggaaatta ctttttcttc
    20521 atgataactc ccttgttcat ctatataggt caatacacca taatgttcgg gcaaatcgat
    20581 accaaagtct tcatgatcag gcatgtaaaa cattttcaga tgttcttctt gtcgatcttt
    20641 tgtttgtttc acgaacatcc ctttttttcc gtaaacgacg aaacttggac gttctttgat
    20701 gcggaaataa ctggatttta tcgaaatttt catatttcca tagaaaaaat ccagatcgaa
    20761 gtaatcattc attcgtcctt tccctaaaag ttgacgtaca tcatagctga tatcatctgg
    20821 ttcgccgaaa taagaaagaa cttgatctac tgtatgacaa gcatgtccat ataaatagct
    20881 gttcgcagta gaaaattcat gcgtattttc tggtacttcc ggtctgaaat aatcataatg
    20941 catttctact tctaaaatat cccctaattt tccgctttcg attacttttt ggactgtcaa
    21001 gaagtcagaa tcgaaccgtc tgttttggta acattggata aatagacctt tttcttttgc
    21061 taatgcaaat aactcttctg cttcctttga cgtttctgca aaaggtttct ctactagtac
    21121 attcttacca tgttcgagtg ccatttttcc atattcataa tgaaattgac taggtgtagt
    21181 gatgacaact aattggatct caggatcatc ccaaagatca gacagctgat cagtatattc
    21241 gatgtcttcg aatgttttcc acactggttc agatggggaa aaaatttttt tgatattgat
    21301 tttgttcgta agttttcttg aaaaaggcaa atgatagcga tttgtactct ttccattgcc
    21361 aagataagcg attgtaagca tgagtggtgc ctccttttat tgatgttttc agtatatcct
    21421 agaaaagcgc agaaagtatt tataatagct attctggtaa aaaagttcag aaaaacactt
    21481 gttatcattc ggtttggtga aattaatgtt caaagtaagg ataaaaatca aaaagatagt
    21541 agcggaacca agtgtttatc agtaagatag aatttggagg tcagatcatg cttattgaag
    21601 aaaaactaac aaaacaagag ttgtttacca cgacagagaa acggattgtc gattacatcc
    21661 gtaggaatat cgaagctgca gtctacatga cgattgaaga attagcaaaa gctacgtata
    21721 cttcccattc agctatcatt cgtttatgca aaaaaatggg attcagcgga tttaaagaat
    21781 tccgtttcga gctggggcgg gaagttcatc aattgattgc tcagttcaat caaacgattg
    21841 atcctaattt tcctttttca agtgaagata gcactcagca aattgcaaag aaaatggcgg
    21901 atttatcgat ccagtcaata aaaaaagcac aattgcagat tgaacatgaa gatttagatc
    21961 aaatcggtaa agtattgaac aaggcacagc gaatctttct ttttgcaaaa ggtgattctc
    22021 aaatcacggc aaggaaattc cagaataaaa tggtcaagtt gaataagttt ttgatcatgg
    22081 ctgaagaata cagtgattct tcttggaatg cagccaattt gactagcgag gattgcgcca
    22141 tttttattag ctacagcggt cgcatccatc attatgaacg aataatgacc tatttgaagc
    22201 atgtagaggc ccctaccttg ctgattaccg gaaatcagca ttcagaaatg gccaaacagg
    22261 ctgatatgtg tcttgttata tcgcaggagg aatatgattt tgctaaagta gcgactttct
    22321 cctctcaaat tgccttcgat tacgtattaa atacgttatt ctcagtgata tattctcaaa
    22381 attttgaaga gaatatccgc aacttgaaaa acaaacaaga attgatgcaa aaaggccttc
    22441 ttaaagaata tcggaaaact taagaaaaga tcctcattta gtaataagaa agatagtgtt
    22501 tgataggctc tctttgatat catcataaaa acaggctgaa gttttctttt tcaaacctca
    22561 gcctgtttgt ttacggaacg attcgaatag atatattata gaaatgagga atagctaact
    22621 tctgtttctt attttgaacg attgacgaaa aagatatata agaaggagga aaatcagtat
    22681 acatgcagct aacaatccat acaggaaaaa tcgtttgttc tcggatactt tctgtacttc
    22741 tttttgaact tcttttgcat gataaggaac ccggtgccct ctgacaagta agcgatgact
    22801 gttgaccata taaggtgtac aagttaggag tgtcacgaaa tcctgccctt tttcgattct
    22861 cagatctttt gtatcagtcg gttctaccgt cttgatctga tcgacttgat aagcgagtgt
    22921 ccgtccattg atttcgatat agaaagaatc acctaaagtc agtttcggta agtctgtgaa
    22981 caactttgct tgaggcagtc ctcgatggct ggaaataacg gcatgtgtat tttctccccc
    23041 cgtaggataa gacgttcctt ccaataatga ggcacctttt tctagcagca cagtattcgt
    23101 ttcatcaaaa attggcaaat ggacattgat ctcagggatc gtcaaaatac cgatcgtatg
    23161 tttctcaaaa taggttctat cctttttcgc aggttctttt ttcttagtaa agggatctgc
    23221 acctggattt ccaccttctt ttgctaattg ctggtttttc tttgtcatgt tttcttggat
    23281 tttagccatg acttcttcat tttctttgac cgcttgttgc tgataatgag agatgatctg
    23341 ctgatccaga taattattca gcgcgtcgct aacaaatgga taagctagta tccctattcc
    23401 tgagagaagc aagaaaagca tcagcagatc tagaagtttc cttttctttg gtttcatttg
    23461 tgttctcctt gtgatgactg tttgataata aaagaaaaag gagcatttgc gtaatccgct
    23521 gctccttttt ccaattcttg caaaaagact aaatctgact gtgcttgcgt ctagcaaagt
    23581 acagtccagc aatcagtaga aggactactc ctgcaccgat atacacatag attcccttac
    23641 cgcctgttga aggaagtgta cctttgtgtt tatttggtat tttttcagga gaaatcaact
    23701 gtcctgctgt tacataagat tgttcatcga tagtaaatgc tacacggttt gttaatggaa
    23761 catatttttc tggcgctttc gtttcttcca gatagtacgt gccatatttt agacctgtca
    23821 catcgattaa accgttactt gtggttgtaa aaaccgttgc tgattctttc gccgataccc
    23881 agctgacggc ttttgtggat gggtcgatcg ataaatattt cgctgtgtca ctatcttgat
    23941 cacgaacgac gaattctgct ccagcaagtg tttggtctga tgtaacgtca ccatctactt
    24001 taacaaatcg tttgccccca gtaacgacat cgactgacgg tggtgtttga tcatttgtat
    24061 gcccgttatc gacatttgct tggttagaaa atcctttggt tggatctgct ttttcgttca
    24121 aatgcatata gtaaacgaat ttcaatgtac cgccaggagt taatgaagga atataattcg
    24181 gatcaactga aaccgtgaat ccgtctgttt gctcagtgac agaataattt actgggtcga
    24241 tttctttatt tccatcatat aaggcataag catacgttcc tgaagaatca ttatcaaatg
    24301 ttaaagcagc gtcatgagta tcgataagtt tgaatgttgt gtacttgttt tgcgttcctt
    24361 ctttatcagc gatacctaat gggatattga cagaaatctc atattgaatt ttttcaccga
    24421 ttgcgacatc ttttccattc aattgaggtg ttgttttatc aactttagac gtatcatttt
    24481 tgatggtctt ttctactggt gtttggctat ttgccaggat ctcaaaaggc gtgattgttt
    24541 gcttttcaat catttccgca ttatcaggag cttttacttc ttctaaatta tattttccaa
    24601 cttctaaatg attaacgatc aactggcctt tttcaataga tgcctcggca aagtcattgt
    24661 tgccgatgtc ataagaatga ccagtaatga aatgtttggc tttggtttga tcagttgtcc
    24721 aagtgtacaa tccatctgtg acactttgga tgtatttttt gacgcttggt gttccttctt
    24781 ctttagaaat aataaattct gctccattta gtgcttcgtt ttcggcagta ccgatttttg
    24841 taactttcaa cgttccatca ttaccgactg tatttttagg gtagagatgg atagtatcta
    24901 gttcttctgt cccgtattta taagagccat ctgcttgttt gatcatctca tatacaggga
    24961 aagctaaaac catgtttgcg gcagctgaca ctccgtcttt tggttcttct ttgatcgtgt
    25021 agactgcatc tttcccattt tgttttttag gtaaagataa agtgacattt ccatcagcat
    25081 ctgtcgttcc tgaagcaact ggtgtaccag gagtcaaaga ctggactgct tgttttgctg
    25141 catccacgga cgctccttta tctcgttgcg cataaaattc ttgagtgacg ttataaactg
    25201 aaaatgaaat atcggctaat ccttggtatt gatcgaattc gctcatttct ttcccgctgt
    25261 tttggattaa aggatcgggt aaatcagtca ttttcttttt atgaaggatg acttgagctg
    25321 ctgtctcctc tgcaaacact ttttttgcgc cgaagctgtt tgtgagtaat ggtaaaataa
    25381 ggaaaaggac tcctaacata acgtttattt ttttatgatt tttcattttt tcactcctgt
    25441 tctttttttg ataaacaacc agattgcaaa gcttgcgaat accataccaa caacgcctaa
    25501 aagtgtattt ttcgtttcgt ttgtttttgg aagaaatgga cggctatttt cttttttatt
    25561 gactactttc aatatactgt ccgcctcttt atcctcagaa agaaaagttg aaatggtaaa
    25621 cggaatttct gtttcactta aaatataacc agaaggtgct tcgatctctt ttaatcgata
    25681 acttcctgtt ttcaacccgg aaattgaaaa agcgccattt ttatcggaaa tcaggtgaag
    25741 cgcatctgat tcgttctttg tccacttgta tccgttggct gtttgctgga ggtaatttcc
    25801 tgctttgttt ttaataagga atactgcgcc agctaatcgt ttatctggtt tatttgtctc
    25861 gattttgaca aattgcttac tccctgtata tacagagact ttctttgtac tgacaccaaa
    25921 accaaaatcc agtgttccgt tgttgttgat ttccttgttc gcctgtgctg tactgcttag
    25981 ctgcatctga taagaaatgg tcagtttttt tccagcatat ttttccaacg aagggatcga
    26041 aaataaaaca tcaaatccgt gaggtttctt gtgtatgaca aagccttctg tcagcttttc
    26101 tccattcgat gaaatcgtta aactattagg taaaaacgtt aatgcttcat ccgcactatc
    26161 tgacaattcg aacttttgat agtcaaggat atttacagga attgtcgtat gcaaggaata
    26221 agtgattttt tcaccaatcg taaaatcatt tctaggctct tctaatacct tctcaaaagg
    26281 tggatcaggg tagtcgtttt cttcattttt aggatacaaa tggatagttg aaagtttttg
    26341 attgttttgt ccatgtacag gaagaacaac taccatattc tctgcctttt ctttgacgac
    26401 ttctggtact ttggattcaa tgaaaagata cgctttatct cttttttctt gatctttagc
    26461 ggccagagaa aaagaagcga taccatcttc gttgttaaga gttgtagttg ttgcttctgc
    26521 ggtaaacata ccggaagacg caccgttttt tgcgatctct gcttgtgctt cttctaccgt
    26581 tttgcccttt tcccgtagat ggtaaaaaga atctgtgaca tcataaactt ggaatgtgac
    26641 accatttaat cctcgatacg tttgtaataa agctttttct tgtccgtcat ttggatgatt
    26701 tttcggcagt tgcccgtttg ggaaaagcaa tttgtgtaaa gtaatctgaa ccatttctgt
    26761 ttctgtttct gttgctacct gagtaaaagc tggtttaaat agtaacaaga agagacacat
    26821 actaagccaa ccaagttttt tcatatttag ctgccccctc tttctttttt caaaaaaaga
    26881 taccaaatag aaaacgcaca accaatcatc cctactagat agattcctaa acggccaatt
    26941 cctcccgttt caggtaatgg tacttttgcc tgattcgtga tgtctaaaga aatctggttg
    27001 tttttggctc ctggtgacag aacagattca tgatcttgtc catccacttg aattgaccca
    27061 tcttcgtgta taactatagt aactggctct tttagacctt ggtatccttc tggtgtaaaa
    27121 gtttcggtca gcgtataagt tccaggtttt aaattttcga atagaaaggt atctgtttct
    27181 tgtccatctt ttggcgattc taagtccatt tctggtccct gcagtctgaa ttttgctcct
    27241 tttaatgtct gtccgttgtc ttcttttttg tgtaccgtta gatcaaacgc ttttagtcga
    27301 ttttgatgag tgagcgtcca tacattattt gtcatctcgg tattttttcc cgtgtaaatg
    27361 atttttccat atttatcgat ttggaattca taatttcccg gcgaagtatc atatccggca
    27421 ggaccagctg tttcaacgac tcgatattta ccaggtgtga gtgaatcaaa actggcaaga
    27481 cctttttcat tcgttgtttg gctgtcaatt ggctgataag taccatttgc ttcttttttc
    27541 tgtagggaaa atgctgctcc tttaagattt atctctttgt ccgtcccttg catcgcatac
    27601 ttacgtactg ctacaggaac ttcaacaaaa ggattttcaa tagtcaactg gatcgtatca
    27661 tcggaagtag tgactgtttt tccatcaatg gttaccgtac catcagaagc gatcttgatt
    27721 tcccaagtag tctttttgct taatccatgc ccttctggag cttttgtttc tgtcagcgta
    27781 taggtcattt ctttttgcaa tttgacattt tctggaagag aataggttcc gtcgccatga
    27841 tctgttagta aagtatcaat agaatctcct gttaatttga aaacagcgcc aataagatcc
    27901 ttttcacctg tagaggaatt tttcgttatt ttcaagttta acggtgtgaa ttgtttagta
    27961 tttttccatg tcattgcatc tatttgttga gaatcgtatc ctggtacagg taattctttg
    28021 attgtttggt aactgaatgc ttgaccttga ttattgtatt gaggtagtga taatatctct
    28081 tgataacttt ctccgctatt tgcagataat ttgtcaatgt ctgcacgttc ccacgtattt
    28141 gttgtatctt tagctggttt aatgattcga atatatccgt ttttccaagc tgcagcattt
    28201 gtcgtatgtt cccgttgaat ctcaaaagta acttgatctg gacgatcagc tagattgttg
    28261 tcaaattctt cccataactt tttgatgtga agactgactc cgggagcttt agcagatggt
    28321 ataccgaatt cagctaattc attggtatca atgtttggct ggaaagttgt cctgccgttc
    28381 atttgatacc agaaatttgg atggaagtcc tcattttctg tttggattct cacttggtaa
    28441 tggatttgga tttcttggtc ttttcctaaa tagatctgat tgctcttgat ggtatttcct
    28501 tctatggaaa tagatggagt gaccgttgta ggacttgtcc ccacactctt gactgaaagt
    28561 gtaccaggct gataaatgaa tggttctgcg attggatcat ttatttgtcc attgcttaca
    28621 gttgctgaga tctgtacagc ttttttggct aggtattcag agatatctgt tgcatgatca
    28681 gctgattcat agtaaagatc gcctttttca tctgatgaaa ccatttgtcg catacgagac
    28741 tctacttctg cttttgagag accagctgcc ggatcgcttt gaagttggat gccaagacca
    28801 tgtatttcga ttcctcgttc tttgagtgcc atcgcttctc cgatggttgc gataaacgta
    28861 ctgtcgattc ttctggatag attgttttgg tcaggtgcat catagattct tgagattaga
    28921 gaagtatttc ccggccgatc ttgcgtatta gaaaactgag ttccgtaata attgctgctt
    28981 gattgtgcgt gtacccgctg tactttatag gaaaatgttg gtacaccatc cgtcagcaaa
    29041 acgatcactt ttttatgtcc atttggaacg gatagcatgc ttcctgcatc tcttagtgct
    29101 ttttgtgtaa aagtaccacc atttgtccgt gaaggtgtaa tggattttac ttgatttttc
    29161 actgaatcaa atgaacccat ctgtactgca ccgttactgt agctatatcc ttcgcttgag
    29221 tagccgacat atcccatatt gattttgtct gtgataccgc tatctgctaa agtatcgaca
    29281 aaacgatcga caccaatctt tacttcaccg atccgattat tgtcgttcat acttcctgac
    29341 cagtctacga ccaatacgag atcaagaggc gtgatatctt tttgtacatt tccacgagca
    29401 ttcaaataaa catcaaacaa tccgggtgta cttgtttcct ttgcatactt tcgtatcgca
    29461 tagtctgctt gattaccggt tcctccatat tcgatatacg aatgggtccg atcattagga
    29521 tctccattcc aagaatttat accatcccat tgatttgttc cgtttttatt gccttgatga
    29581 tcaagaacat ttgtatttcc tgtaggttgc caactggctt taggataagt gccctgatca
    29641 tctgttgtat attctgtagg aatcgtttga ttagatggca cactcacagc tggcaaggat
    29701 aatgtaggca atgtaaatgg gctgacagaa ttttcgtttt cttttgatga ttcttcttct
    29761 atttcgtctg gctttggtac tgacacatct gtatctttct tattttctgt ctcatcaata
    29821 atgtcatatt ctttattatc gacgatcagc ttgtttgcct ggctgtggta aagtgctgga
    29881 tcgattggaa aactcagatc gatagtttgt ttagtgtttg atgagatttc caacgtaatt
    29941 ttcccgctat ttacctgata agcatagcca tctccttctt ttctttccac atcacttaag
    30001 taattttcga ctagaccaat ttctttttct atcttttcag cactggtgtt attcgcattt
    30061 agcttgatgt tcagtgcttt ttttccagac tgatccgaaa tgacttcagc tgaaattgtt
    30121 tctaaaactg ttggatcatc tgtagtgtct gctattgcag ataatggcac aaagtttgat
    30181 aaactcaata ttatcagcat gaaaataact ttcagtttct tccctgtggt cgtcattttt
    30241 cccctccttt ttcttttttt ctgttttgac gtgaatagtg aaaattatta aaataaacag
    30301 gcgttacata attttattta ttttaatatg ataattatta tacgaagggc gctaaaggga
    30361 atcacaccaa ttttcatacc aattggtttc atttatgaag ttgattcctt cgattaatga
    30421 gcgtttcatt gaaagaaaag ggttgaggga gaaaaacata tgatcaggtt gttggaagga
    30481 aactatagaa agatggaaaa aatcattcga attctgtcca tttctgacaa gtggatgact
    30541 aaaaaagaac ttgccaaacg aattggtagt tcagaatcta catttgtaag gtatatcgaa
    30601 gagatcaaaa tccgctgggg aacggatgta acgattcaat cttcacaaaa attggggtat
    30661 cgtcttgaac atttgaatgt accagcttat ttaaatgttc tgatggaaat gtcgcaaagt
    30721 tctacaaaca gtcgtttatt aaatgagatt attgtcaatc cgggtaaaac gattgagtat
    30781 tattgtgaaa aaatttccat cagccggtct tcttttacac gtaagttgaa acaatgcaat
    30841 caaattttag aaagctattt attaaaagtg atcgttgacc aaggttttca gttaactagc
    30901 cttaaaagtg agctccagtt gcgtatcttt gttacctttt tctttttaag ttactacgga
    30961 agacgcacgt tgccatttcc aatcgaaaaa aagaagatca aagaaattat ggtcaagaat
    31021 aattgtcaga tgaatttgtt tcaggaagaa agcccatatg agcaatcttt ctttattatg
    31081 tattttgttg tgcatctcat gagagaatcc caaggaactc catttgaatg gaaagctgga
    31141 gactcttatg tacagttgat aaatatcgat aaaaaagact tcttaatctt gaaaaatctc
    31201 ttaggaaaat tgaagtacag cagctatata aaagtagtga actattttgt caatacgatt
    31261 ttttcagaat tttcggagaa agaaaaaaat gaaatcagtg agcagatacg aaaaaatatg
    31321 tcctactgtt cgttttcatc cgctgattgc aaagaaaaaa acgaagaata tgttgttcaa
    31381 ttattaacgc aaatttattt tttctcccgt attttcccat acgatacgac tcatttgacg
    31441 atccgtgcaa cttactttgc acagcagatg caacaccgcc agatacgtct atatgctgga
    31501 tttcgtgcag aacttcaatt ttattccact ctactgaatc agaatctgat ttattattta
    31561 tctagctata tcttttggat gcttatcggt aatcctgaaa ttcaccatta tacttctgat
    31621 aaaaaaatac tgattatcag cgacttaagt cttcgacata gtcaatatat tgaagaatat
    31681 atcagtgata ttcttgctgt gcataaaatt cactctgaga caactgctat tactgaagat
    31741 cagctgtcga aatacaatct tctcgagtat gatctgatcg ttacgaatca acccattttg
    31801 aatgcccatg ttcctcatat attgattgat gatagtgtgt cattcgcaaa tgaagaagaa
    31861 ctcatccgct tattcgagtt atgaataatc aaaaaacagc tatgtataaa ccaccctgat
    31921 aaaaatttca ggacaaatac acatagctgt ttctttttat tgtattcctt cagcaatcca
    31981 acgagattta tagcgaaagt cttttaacaa ctcttctttt gtccaattat ttgcaatgag
    32041 atccgtcaaa gtgttcatta cgagggtcat ggaaaagtat aggaaactaa ctgcttcttc
    32101 cgacgattga atggagaaat gattgattct taataattcc agccattttt cgttcattgg
    32161 agaatcagcc gatagttctc tacgtttggt tgtctgctga tcatttcgct gggttaacaa
    32221 gtataacctt tgccaataga tactttcgtg atctaagtcg cagcaccact ctaaatattt
    32281 ttcgatcccc aaaaagaaat gttgtttgtt ttcgctaatg aattttaaga tatcttgatg
    32341 gactatgtga gaactgcttt tgatcgtata caaataagca tcttccaaat cttgaaaata
    32401 tttataaaaa gcaccgcgcg acatccccat cttgtcaacg atttctgaga ctttgacttg
    32461 gctgatgtgc tgtgagcaaa atatctccag taataggtca tcgattctct gtttctttgc
    32521 gttcgataaa ttgaaaaaag tttcttttgg catccacatc tctcctttat ctctgattat
    32581 acgtggatag cccttcattg tcatgaagga ttcattcctt ctttttctct tcttctgtca
    32641 agtacccatc ttccattcga taaacgcgat cactccattg aatcatacgg acatcatgag
    32701 taaccataat agtcgctttt tgtttctcat gagcttcttt aactagtaat ttgacgactt
    32761 catatgcatg atctgtatcc aaactagctg ttggttcgtc tgctaagatc aagcttggct
    32821 cattatataa agcgcgagca attgccactc tctgtctttc tccaccagaa aggtctttag
    32881 gataactatt tttaagtgac tggatatcta aagattgcag caaggaatcg atttttgttg
    32941 aatcttcttt ttttgaagta acttggtcaa ctagttcaaa ttgttccttt accgttaaaa
    33001 aaggaatcaa attagagctt tggagaataa atcctatttc tttaaagcgt aaatctgctc
    33061 gttttttttc tggaagctca gttagtgagt gaccgttgat cactatttct ccggaagtcg
    33121 gtgtttgcaa tcctcctgaa atggtaagga aagtactttt acctgaacca gaaggaccaa
    33181 tgatgctgac gaattctcct tcctttacat aaaaatcaat tgctttcaaa gctgagacct
    33241 ttgtatgacc agaaccaaat gttttggtaa cttttttcat cgttaatatt tttttcattt
    33301 ttattaacct cctattgctg taattgggtc tactttggtt atcgtacgta tcgaaaacaa
    33361 tccgcctagc attgcgacaa tgatcaaaat cccactatat agcaaccatt gtgaccaaaa
    33421 aacagcaaaa ggcatcgcgc ttggtaaaat cagactagtc aaataagcaa acaaaagagc
    33481 aagcagaacg cctaccaccc caacaataaa ggactgtgct actaaagatt tagcgatgaa
    33541 gaaatttttg atcccttgtg ctttcattac tccaaaaata gcagtcttct gaagtgtcat
    33601 cacgtacatg aaaataccga ccacggcagc aaccactaag aacaaaaagt atatcatcgc
    33661 atttaaggtc atattttggg cagaatagcc aggaatactt tcaatgaatg tcccaatagt
    33721 taataactgt agacccttcg atgctttttc tttcgaaatt ttagctgaat cctttattac
    33781 gatcccattg atcggctgtt cttcttcaga agcaaacggc tgttgtccat acttcaaagc
    33841 agtccacgta tctagggacg catagatgac gggagaaact gtgtaataag tttcaggaaa
    33901 gataccgaca atcgtcaaag gatcgtcgta actgccaatt ttgatcgtat ctccaatttt
    33961 atagcctgaa tcagccagat tttgagagat gatgatttca ttctttgttt taaattcatg
    34021 ccctttcgtg agttttggta ataaaaatgc ttctttcgtt gtgccgaaaa ctgtaacatt
    34081 ttctttgttt tttcctttga ttgctccgct gtacaatccg attggtgctt tctctcctcc
    34141 ttcaatgtat ttcagatctc ctcttgttaa ttgtgaagct gcaaatactt gatttgcatc
    34201 ctccgacaaa acaatctctt gtgcgtccca atcatcgatt gcttttttaa actcttctga
    34261 aagtccattc gctagtcctg acaacatgaa tacgacatac gcaatcaaga acataatacc
    34321 gacgatcaag ccatatcgta atttagaata gcgaatttct ttgatagcta gaaacatttg
    34381 tttttcctcc ctatgataga ttgatagtga caaaatgtca cttgttgaat agtgtaactc
    34441 taaagtgaca atttgtcact tgtgttgttt ttttcttatc aataaattgt ctaagacatc
    34501 tttcgataaa accttaacat gagtcataaa aaatttattt aagccttatt tatgctttcc
    34561 atgataaact aaagtgacca aatcaatgag gtgggaaaat gattaaacta ttagtagtag
    34621 aagacgataa agtattatcc aataatataa aagaaatact cgctgcaatc ggcgagataa
    34681 cacaagttta tgatggtagt gaaggactgt tcgagttgga aagtggagtt tatgatcttg
    34741 caatcttgga tttgatgatg ccggaaatga atggatatga agtgttagca aaaatacgga
    34801 aagaaaaaat acagactcca gtattgattt tgactgcaaa agatagtttg gaagataaga
    34861 ttgaaggatt tgaacgaggt gcagatgatt atctaacaaa acctttctat cgagaagaac
    34921 tgatcatgcg cgtgaaagca ttattgaaac gctcgctagg tctctttgat gaaaatgttc
    34981 tgaagtataa ggatatcagt tgtgatttaa aaagcaatga agtcttgtat aaagaacaag
    35041 ttttgcccat acaaggaaaa gagtttgaat tgctcgttta ttttttacaa aataagggaa
    35101 tcattttgac aaaagagcag atatttgacc ggatctgggg atttgattct gatacgacga
    35161 ttacagttat tgaagtttat atgagcaact tacgaaaaca tctgcgaagt actggattgg
    35221 ataaagaaat caaaacctta cgtaatgtcg gttatatact gcaaagggtg tgatgcaatg
    35281 aatggagata ttacacgaaa gcaacgtagt cgcttttttg cgacaaatgt agcagcattt
    35341 gcttttatct tttttctttt gggattcatt actttacaga tattaaacca atctgcatac
    35401 agagaaacag atctgtcatt agagaaaata aattttgatt ctcgaacagt acaattagag
    35461 atacaaagat atcagcaagg cgatcccttt ttaaacccga ctcaaacgga acaaaacaga
    35521 acaaacatgg aacagccatt aatggaacca tcaaatgacc gttttaacac tcaagtgatc
    35581 ttgtggtcta aaaatggtga gatattgaat aaagcagcta ttggtgggcg tttcaaccaa
    35641 atcaaagaat taaaaatgga tacgaaaaat ctgaatacca tccaaaatat tgaattaaat
    35701 gaagacagtg gctcatctgc cctctctttc cgttcgatta cgaaaaaagc ggagacgaat
    35761 caagatggcg ttgcctacat ccaaatttta gaaaatacaa atcaaatcaa gaattcatta
    35821 gcgacttttc gcacgattat gattttgtgt atgattgttt tttggattct ctctattggt
    35881 atcagttatt acttgtctag tctaagcatg cgccctatta ttctttcttg gagaagacaa
    35941 aaagaatttg ttgagaatgc ttctcatgaa ttgcgcacac cgttaacgat tattcaaaat
    36001 agtttggaac atctttttac caagcctaat catactatta ttgaagaatc cgaaagcatc
    36061 gctcaagcat tgtcagagac gaggcgcctc actggattga cttcggattt gctgacaatc
    36121 gctcggaatg attctgatca gcagctgctt tcaaagcaaa tgatcaatac acaagaatat
    36181 atcgaacaac ttgttaaacc atttcaagag atggccatca tagatggaaa agagtttatt
    36241 ctccataacg atgcaacatt agaagtacca attgatgaaa aaaaaatcca tcaagttttg
    36301 gttattttgc tggataatgc attaaaatat acgaatccag aagataagat cattttggat
    36361 tcatctgtta accataaaga atggctgatt cacgtaaaga atactggccc ttctatcagt
    36421 gaagaagatc aagcaaggat ttttgatcga ttttatcggg aagatcaatc tcgggcgaaa
    36481 gagactggcg gatacgggct tggcttggca attgcaaaac aggtaatcga acagcatcat
    36541 ggacagattt ctgtatccaa ttatcttcca caaggagtcg attttaaagt ccaattgccg
    36601 ttgaagccta aaaaataatg cttatcccta aaaaagggct gtgacaaaaa tcttgtcaca
    36661 gccctttttt gttgcgaata aacggtgttc cagaagtagc ttcttcggaa ataagccgaa
    36721 atttctttaa gctgaccact tctgtcacaa cctctttagc ttacgcttac gattttttat
    36781 tgcggtcttc aatgtgtaca tcaccgcttg tgtcaatatc caattcttct cggctaacat
    36841 cttctgtcac tttttcaaca tcttcatgtt cttctttatg aatcccaact tcttcacgaa
    36901 taactgtatg tttgcttaca tctacttgct cttctttgat tgggatagta atcgtgtcat
    36961 cttccaattt ttcgtctgtt ccctgtgaag attgatctgt tactggtttg cgttcgatga
    37021 cgatttcttc acgtttgacc ggtacttcta ccgtttcagt atcgttgacc acatgtttgt
    37081 ggatgtcgac ttctccagtt gtcacatcat gtgtatttac atctaatttt tcttctttta
    37141 gttgaattgt atcatcatct agatttggtt tgcgatcgat gtcttcagtc ttgcttgtac
    37201 ccattgtatc agtctcgcca ggtacgtcca tcggatcagt tcctggtgtc atattcaatg
    37261 gatccgttgt attttgaaag cctgtcgtgc cacctacgtc cattggatct gttccagtgc
    37321 cagtttctaa tccagtcccc attgtgcttg ctgggctaac agtatcttgt gtatccattg
    37381 tatcttctgt tgtttctggc tgacgatagc cttttactgt gatgacaaat tttccatcag
    37441 aaatatctcc tctatattga tataaaggat cattttctgg tgtagccgtc tcagaatcat
    37501 aggtatcaaa agaaaaagta tcttttattt tttcccataa agaacggtct tcttcaccat
    37561 ccatccgatt ttcatctgtt tcaacatcta taccagacaa tccgtcaaat cctagtctag
    37621 atgctgcttc atcagtagta taaataacga tatcatctcg ttcataccct tcgttacgca
    37681 accgttcaat tactgataat gcttcttccc tcgtactata gcttccgatg accgttaaat
    37741 ttttctcttc catgtaaatt cctcctagca aatagattta tgattggtat attgtt
//
LOCUS       DASJPC010000017        37728 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJPC010000017 DASJPC010000000
VERSION     DASJPC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.017"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            336..1439
                     /locus_tag="U4A13_000852"
     CDS             336..1439
                     /locus_tag="U4A13_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN1779172.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="U4A13_000853"
     CDS             1723..2403
                     /locus_tag="U4A13_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1779173.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="U4A13_000854"
     CDS             2493..3527
                     /locus_tag="U4A13_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1779174.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="U4A13_000855"
     CDS             3541..3876
                     /locus_tag="U4A13_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779175.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="U4A13_000856"
     CDS             3860..6340
                     /locus_tag="U4A13_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779176.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="U4A13_000857"
     CDS             6396..7955
                     /locus_tag="U4A13_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779177.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="U4A13_000858"
     CDS             7952..9316
                     /locus_tag="U4A13_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1779178.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="U4A13_000859"
     CDS             9487..10557
                     /locus_tag="U4A13_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779179.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="U4A13_000860"
     CDS             10572..11240
                     /locus_tag="U4A13_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779180.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="U4A13_000861"
     CDS             11260..11838
                     /locus_tag="U4A13_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1779181.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="U4A13_000862"
     CDS             12241..13152
                     /locus_tag="U4A13_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1779182.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="U4A13_000863"
     CDS             complement(13408..14325)
                     /locus_tag="U4A13_000863"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN1779183.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="U4A13_000864"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="U4A13_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN1779184.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15569
                     /locus_tag="U4A13_000865"
                     /pseudo
     CDS             15022..15569
                     /locus_tag="U4A13_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            15684..18044
                     /locus_tag="U4A13_000866"
     CDS             15684..18044
                     /locus_tag="U4A13_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN1779185.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18131..18445
                     /gene="trxA"
                     /locus_tag="U4A13_000867"
     CDS             18131..18445
                     /gene="trxA"
                     /locus_tag="U4A13_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN1779186.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18627..20405
                     /gene="uvrC"
                     /locus_tag="U4A13_000868"
     CDS             18627..20405
                     /gene="uvrC"
                     /locus_tag="U4A13_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN1779187.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20550..21449
                     /locus_tag="U4A13_000869"
     CDS             20550..21449
                     /locus_tag="U4A13_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779188.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21442..22704
                     /locus_tag="U4A13_000870"
     CDS             21442..22704
                     /locus_tag="U4A13_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779189.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22867..24765
                     /locus_tag="U4A13_000871"
     CDS             22867..24765
                     /locus_tag="U4A13_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN1779190.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25064..25783)
                     /locus_tag="U4A13_000872"
     CDS             complement(25064..25783)
                     /locus_tag="U4A13_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN1779191.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26120..26452
                     /locus_tag="U4A13_000873"
     CDS             26120..26452
                     /locus_tag="U4A13_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779192.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26439..28442
                     /locus_tag="U4A13_000874"
     CDS             26439..28442
                     /locus_tag="U4A13_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN1779193.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28461..28931
                     /locus_tag="U4A13_000875"
     CDS             28461..28931
                     /locus_tag="U4A13_000875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN1779194.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28952..29536
                     /locus_tag="U4A13_000876"
     CDS             28952..29536
                     /locus_tag="U4A13_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN1779195.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29551..30537
                     /locus_tag="U4A13_000877"
     CDS             29551..30537
                     /locus_tag="U4A13_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN1779196.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30527..30838
                     /locus_tag="U4A13_000878"
     CDS             30527..30838
                     /locus_tag="U4A13_000878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN1779197.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30860..32641
                     /locus_tag="U4A13_000879"
     CDS             30860..32641
                     /locus_tag="U4A13_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN1779198.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32634..34010
                     /locus_tag="U4A13_000880"
     CDS             32634..34010
                     /locus_tag="U4A13_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN1779199.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34017..34652
                     /locus_tag="U4A13_000881"
     CDS             34017..34652
                     /locus_tag="U4A13_000881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN1779200.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34682..36037
                     /locus_tag="U4A13_000882"
     CDS             34682..36037
                     /locus_tag="U4A13_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN1779201.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36229..37623)
                     /locus_tag="U4A13_000883"
     CDS             complement(36229..37623)
                     /locus_tag="U4A13_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN1779202.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT        12456 a         6459 c         8052 g        10761 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg
    15481 agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg
    15541 agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15601 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15661 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15721 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15781 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15841 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    15901 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15961 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    16021 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    16081 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    16141 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    16201 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    16261 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    16321 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    16381 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16441 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    16501 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16561 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16621 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16681 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16741 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16801 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16861 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16921 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16981 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    17041 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    17101 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    17161 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    17221 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    17281 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    17341 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    17401 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17461 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17521 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17581 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17641 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17701 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17761 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17821 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17881 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17941 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    18001 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    18061 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    18121 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    18181 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    18241 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    18301 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    18361 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18421 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18481 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18541 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18601 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18661 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18721 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    18781 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18841 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18901 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18961 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    19021 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    19081 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    19141 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    19201 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    19261 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    19321 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    19381 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19441 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19501 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19561 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19621 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    19681 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19741 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19801 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19861 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19921 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    19981 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    20041 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    20101 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    20161 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    20221 gtaaaaacag ttttgcttcc cgattagatg aaattgaaag gttaggacca aaacgaaaaa
    20281 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    20341 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    20401 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20461 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20521 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20581 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20641 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20701 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20761 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20821 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20881 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20941 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    21001 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    21061 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    21121 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    21181 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    21241 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    21301 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    21361 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21421 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21481 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21541 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21601 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21661 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21721 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21781 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21841 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21901 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    21961 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    22021 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    22081 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    22141 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    22201 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    22261 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    22321 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    22381 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    22441 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22501 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22561 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22621 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22681 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    22741 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    22801 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    22861 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    22921 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    22981 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    23041 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    23101 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    23161 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    23221 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    23281 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    23341 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    23401 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    23461 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    23521 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    23581 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    23641 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    23701 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    23761 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    23821 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    23881 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    23941 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    24001 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    24061 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    24121 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    24181 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    24241 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    24301 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    24361 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    24421 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    24481 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    24541 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    24601 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    24661 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    24721 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    24781 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    24841 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    24901 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    24961 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    25021 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    25081 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    25141 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    25201 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    25261 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    25321 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    25381 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    25441 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    25501 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    25561 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    25621 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    25681 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    25741 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    25801 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    25861 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    25921 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    25981 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    26041 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    26101 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    26161 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    26221 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    26281 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    26341 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    26401 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    26461 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    26521 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    26581 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    26641 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    26701 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    26761 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    26821 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    26881 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    26941 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    27001 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    27061 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    27121 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    27181 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    27241 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    27301 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    27361 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    27421 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    27481 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    27541 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    27601 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    27661 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    27721 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    27781 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    27841 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    27901 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    27961 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    28021 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    28081 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    28141 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    28201 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    28261 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    28321 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    28381 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    28441 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    28501 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    28561 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    28621 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    28681 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    28741 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    28801 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    28861 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    28921 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    28981 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    29041 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    29101 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    29161 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    29221 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    29281 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    29341 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    29401 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    29461 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    29521 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    29581 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    29641 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    29701 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    29761 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    29821 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    29881 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    29941 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    30001 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    30061 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    30121 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    30181 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    30241 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    30301 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    30361 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    30421 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    30481 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    30541 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    30601 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    30661 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    30721 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    30781 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    30841 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    30901 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    30961 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    31021 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    31081 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    31141 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    31201 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    31261 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    31321 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    31381 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    31441 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    31501 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    31561 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    31621 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    31681 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    31741 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    31801 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    31861 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    31921 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    31981 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    32041 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    32101 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    32161 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    32221 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    32281 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    32341 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    32401 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    32461 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    32521 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    32581 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    32641 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    32701 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    32761 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    32821 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    32881 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    32941 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    33001 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    33061 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    33121 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    33181 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    33241 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    33301 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    33361 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    33421 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    33481 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    33541 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    33601 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    33661 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    33721 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    33781 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    33841 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    33901 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    33961 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    34021 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    34081 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    34141 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    34201 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    34261 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    34321 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    34381 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    34441 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    34501 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    34561 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    34621 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    34681 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    34741 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    34801 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    34861 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    34921 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    34981 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    35041 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    35101 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    35161 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    35221 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    35281 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    35341 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    35401 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    35461 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    35521 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    35581 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    35641 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    35701 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    35761 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    35821 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    35881 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    35941 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    36001 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    36061 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    36121 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    36181 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    36241 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    36301 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    36361 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    36421 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    36481 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    36541 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    36601 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    36661 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    36721 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    36781 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    36841 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    36901 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    36961 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    37021 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    37081 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    37141 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    37201 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    37261 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    37321 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    37381 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    37441 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    37501 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    37561 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    37621 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    37681 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaa
//
LOCUS       DASJPC010000018        37535 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJPC010000018 DASJPC010000000
VERSION     DASJPC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.018"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..470
                     /locus_tag="U4A13_000884"
                     /pseudo
     CDS             75..470
                     /locus_tag="U4A13_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="U4A13_000885"
     CDS             complement(465..683)
                     /locus_tag="U4A13_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779203.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="U4A13_000886"
     CDS             790..1104
                     /locus_tag="U4A13_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779204.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="U4A13_000887"
     CDS             1208..2386
                     /locus_tag="U4A13_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1779205.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="U4A13_000888"
     CDS             2789..3082
                     /locus_tag="U4A13_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN1779206.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            3925..6252
                     /locus_tag="U4A13_000889"
     CDS             3925..6252
                     /locus_tag="U4A13_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1779207.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6378..6707
                     /locus_tag="U4A13_000890"
     CDS             6378..6707
                     /locus_tag="U4A13_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1779208.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            6747..7751
                     /locus_tag="U4A13_000891"
     CDS             6747..7751
                     /locus_tag="U4A13_000891"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEN1779209.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            7748..8194
                     /locus_tag="U4A13_000892"
     CDS             7748..8194
                     /locus_tag="U4A13_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEN1779210.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8344..9159
                     /locus_tag="U4A13_000893"
     CDS             8344..9159
                     /locus_tag="U4A13_000893"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1779211.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9365..10537
                     /locus_tag="U4A13_000894"
     CDS             9365..10537
                     /locus_tag="U4A13_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1779212.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10699..11193)
                     /locus_tag="U4A13_000895"
     CDS             complement(10699..11193)
                     /locus_tag="U4A13_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779213.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            11776..12198
                     /locus_tag="U4A13_000896"
     CDS             11776..12198
                     /locus_tag="U4A13_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779214.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12364..13557)
                     /locus_tag="U4A13_000897"
     CDS             complement(12364..13557)
                     /locus_tag="U4A13_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1779215.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            13781..14158
                     /locus_tag="U4A13_000898"
     CDS             13781..14158
                     /locus_tag="U4A13_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1779216.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14146..14484
                     /locus_tag="U4A13_000899"
                     /pseudo
     CDS             14146..14484
                     /locus_tag="U4A13_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14474..14587
                     /locus_tag="U4A13_000900"
     CDS             14474..14587
                     /locus_tag="U4A13_000900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1779217.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            14618..15061
                     /locus_tag="U4A13_000901"
     CDS             14618..15061
                     /locus_tag="U4A13_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEN1779218.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15322..16137
                     /locus_tag="U4A13_000902"
     CDS             15322..16137
                     /locus_tag="U4A13_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1779219.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16147..16809
                     /locus_tag="U4A13_000903"
     CDS             16147..16809
                     /locus_tag="U4A13_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1779220.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17017..18681
                     /gene="alsS"
                     /locus_tag="U4A13_000904"
     CDS             17017..18681
                     /gene="alsS"
                     /locus_tag="U4A13_000904"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN1779221.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            18694..19404
                     /gene="budA"
                     /locus_tag="U4A13_000905"
     CDS             18694..19404
                     /gene="budA"
                     /locus_tag="U4A13_000905"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN1779222.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            19534..20031
                     /locus_tag="U4A13_000906"
     CDS             19534..20031
                     /locus_tag="U4A13_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN1779223.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20216..20476
                     /locus_tag="U4A13_000907"
     CDS             20216..20476
                     /locus_tag="U4A13_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779224.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(20838..21077)
                     /locus_tag="U4A13_000908"
     CDS             complement(20838..21077)
                     /locus_tag="U4A13_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779225.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            21363..21677
                     /locus_tag="U4A13_000909"
     CDS             21363..21677
                     /locus_tag="U4A13_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779226.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="U4A13_000910"
     tmRNA           complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="U4A13_000910"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22648..23421)
                     /locus_tag="U4A13_000911"
     CDS             complement(22648..23421)
                     /locus_tag="U4A13_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1779227.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            23565..23915
                     /locus_tag="U4A13_000912"
     CDS             23565..23915
                     /locus_tag="U4A13_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN1779228.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            23896..24612
                     /locus_tag="U4A13_000913"
     CDS             23896..24612
                     /locus_tag="U4A13_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1779229.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            24612..26060
                     /locus_tag="U4A13_000914"
     CDS             24612..26060
                     /locus_tag="U4A13_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1779230.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(26130..26639)
                     /locus_tag="U4A13_000915"
     CDS             complement(26130..26639)
                     /locus_tag="U4A13_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779231.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(26629..27354)
                     /locus_tag="U4A13_000916"
     CDS             complement(26629..27354)
                     /locus_tag="U4A13_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779232.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(27577..29697)
                     /locus_tag="U4A13_000917"
     CDS             complement(27577..29697)
                     /locus_tag="U4A13_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN1779233.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            29927..31105
                     /locus_tag="U4A13_000918"
     CDS             29927..31105
                     /locus_tag="U4A13_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN1779234.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            31121..31879
                     /gene="aroD"
                     /locus_tag="U4A13_000919"
     CDS             31121..31879
                     /gene="aroD"
                     /locus_tag="U4A13_000919"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN1779235.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            31902..32387
                     /gene="ybaK"
                     /locus_tag="U4A13_000920"
     CDS             31902..32387
                     /gene="ybaK"
                     /locus_tag="U4A13_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN1779236.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(32458..33711)
                     /locus_tag="U4A13_000921"
     CDS             complement(32458..33711)
                     /locus_tag="U4A13_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1779237.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            33829..35178
                     /gene="gdhA"
                     /locus_tag="U4A13_000922"
     CDS             33829..35178
                     /gene="gdhA"
                     /locus_tag="U4A13_000922"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN1779238.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            35290..36636
                     /locus_tag="U4A13_000923"
     CDS             35290..36636
                     /locus_tag="U4A13_000923"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN1779239.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(36944..37330)
                     /locus_tag="U4A13_000924"
     CDS             complement(36944..37330)
                     /locus_tag="U4A13_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1779240.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(37379..>37535)
                     /locus_tag="U4A13_000925"
     CDS             complement(37379..>37535)
                     /locus_tag="U4A13_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEN1779241.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT        12539 a         6485 c         7432 g        11079 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
     3781 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa agccccaaac
     3841 tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa aggattattt
     3901 gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa taaaatccaa
     3961 cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag aaaatactta
     4021 catcccatta tgatttcgat catcttgatt tttactttct tagggagctt atcaagtatt
     4081 tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga ggagacaaat
     4141 agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag caacaacgag
     4201 acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga aatcacagca
     4261 gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac atttagtact
     4321 gttgcggcca ctgcgaattt caacggacat accataccag atggatatac agtgatatat
     4381 gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa aactgcgtta
     4441 caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca taatctgttt
     4501 attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta tatgggggtg
     4561 gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag ttattctttt
     4621 acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt ttcaatcggt
     4681 atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg gcagatcgtt
     4741 gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt tcaggtcaat
     4801 gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa aatcggaacg
     4861 tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac tccaacagca
     4921 gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga aagtaacgtc
     4981 gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca agactatttt
     5041 ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc tgcacaagac
     5101 ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc agcgatagaa
     5161 acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg aaatgtaatc
     5221 gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa gacaaatgca
     5281 acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt tgtaccgatc
     5341 gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa tgatctcagt
     5401 attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa gggagttgaa
     5461 gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa aagcggacta
     5521 gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc tgatgtctat
     5581 acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata tgatggaaaa
     5641 gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc gcttactcca
     5701 gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc aaaaaatatg
     5761 gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa agtcaaagca
     5821 gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta taccatctat
     5881 aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc ccaaagtcac
     5941 tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc attggattat
     6001 accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat tagtttaggc
     6061 gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa gttgacgatc
     6121 aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt tgtcttttta
     6181 ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa aaaagcaggt
     6241 gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt ctctttttta
     6301 tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca cctattacat
     6361 ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt caaaacgacg
     6421 gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt actttcgcta
     6481 tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt gtggaagacg
     6541 cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa aatgtgacaa
     6601 aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa gatgagcaag
     6661 gtcgtgacgc tgtttaccga tttacggaaa taaagacttc agattaagta aaagaaaaat
     6721 cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa ttgacgacca
     6781 tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta acaaaaacag
     6841 tagagaataa gcagacagat tttgcagatg gggataaagt cccttatctc atcaccgtaa
     6901 caattcctga gaacatcaac gagataggaa cctatacgat caaagataca gctgatcctc
     6961 agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag attcatacat
     7021 ttcacacaca aaaaataaaa catggttttg tcttatcaga ttctggaaaa gatcttagtt
     7081 cgtttgctgg aaaaaaatta acgatccgtt accaaatgac attgaaagaa aacagtgaaa
     7141 aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta gaaacacatc
     7201 ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg aaacaaaaac
     7261 tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa gaaaacggga
     7321 taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc agtaaagatg
     7381 gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag attaaaccgc
     7441 ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa ggcagttatg
     7501 atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat ccgacgaata
     7561 atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa aatggaggaa
     7621 gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg ttattgctag
     7681 tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac aataggaggg
     7741 aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg ctatcatgct
     7801 gcttcctttc gttctcggcc taggaactgc agaggtttct gcagccgtat cacctacacc
     7861 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     7921 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     7981 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     8041 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     8101 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     8161 aacgtcgcca aatatcgtgt taagcttacc ctagaccctg acaaaccttt agacacgatt
     8221 cacctatatg ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac
     8281 ttttcagggg tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa
     8341 ttcgtgaccg gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg
     8401 ttgacattca caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat
     8461 tatactttag ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa
     8521 actactttta tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca
     8581 gatgaagcca atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat
     8641 aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat
     8701 gtgaacacgc aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca
     8761 actgttaaat taatgaaaaa aatagcaaac cttgcctcag gagaataccc atatgcttgg
     8821 tcagatgaag tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg
     8881 aaattttcaa tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca
     8941 gaaggctacg cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat
     9001 ccaacaactt ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact
     9061 ggtggtatgg ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg
     9121 atctatttca aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat
     9181 acgataaata aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc
     9241 ttggtattcg ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gcttcgctaa
     9301 tactcaggtc aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga
     9361 catgatgaat aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt
     9421 tgggatcggg atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca
     9481 gcaaatgatc atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa
     9541 acagcaggaa aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg
     9601 gataggcagt ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc
     9661 cttttttatg gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc
     9721 tatattcgat cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaaggaag
     9781 ttcttatccg actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc
     9841 agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca
     9901 gatcaatgga aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgacga
     9961 agtagattct ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgcactcc
    10021 ttatatggtc aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa
    10081 agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg
    10141 gtttatcggt atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga
    10201 ttctttggcc atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg
    10261 acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac
    10321 tagagaaaaa gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga
    10381 tttaaaaggc aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa
    10441 acataaacat tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa
    10501 agaaaaagag gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga
    10561 gggaacagac taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt
    10621 ttggtttact atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat
    10681 tatagtataa cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc
    10741 ttgagcttcg cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta
    10801 ttttgaaccg gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc
    10861 ttgtgcctgc tgagaagcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg
    10921 ctgtgcgatt gcttcttgcc tcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg
    10981 cgcttgttct gcttgttgtc gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc
    11041 ttttgctttg attgtggaat caattgcagc aagacggctt gtcaactctt gtttgttttc
    11101 aggtagcgct tggatagctg ctgaagcagt tgcatagttt tcttttgtag gatttgcttc
    11161 agcttgtgac aaagcagatt ccgcttctgt cacttttttt tctagtgcag ctttttccgc
    11221 tttttgtttc tcttcttctt ttttcttttc aatttgttct ttcgatgaat ctgccgtact
    11281 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
    11341 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaacg caactaattt
    11401 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
    11461 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
    11521 tcccgtgttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
    11581 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
    11641 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
    11701 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
    11761 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
    11821 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
    11881 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
    11941 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
    12001 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
    12061 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
    12121 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
    12181 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
    12241 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
    12301 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
    12361 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
    12421 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
    12481 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
    12541 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
    12601 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
    12661 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
    12721 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
    12781 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
    12841 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
    12901 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
    12961 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
    13021 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
    13081 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
    13141 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
    13201 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
    13261 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
    13321 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
    13381 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
    13441 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
    13501 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
    13561 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
    13621 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
    13681 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
    13741 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
    13801 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    13861 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    13921 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    13981 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    14041 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    14101 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    14161 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    14221 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    14281 caactgaagg agttagaaga gtaactagga acagaactgt ttgtacgcga aaaccgcaaa
    14341 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    14401 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    14461 tacgatcggc tgtgtggaag ctgatagttc gaatacattg gcactattac tagaaaagtt
    14521 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    14581 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    14641 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    14701 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    14761 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    14821 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    14881 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catcattcaa agcgagtgac
    14941 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    15001 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    15061 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    15121 ttgtattttt tcatttttaa atcaaaaaaa taaagagatt tacataactt gttaaataag
    15181 cgcttacaaa aataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    15241 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    15301 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    15361 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    15421 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    15481 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    15541 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    15601 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    15661 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacagat
    15721 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    15781 caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    15841 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    15901 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    15961 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    16021 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    16081 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    16141 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    16201 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    16261 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    16321 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaatagt
    16381 ttttcaccag tacgtttgtt cataaagatg tttcgccaat tttggctaaa gggatttttt
    16441 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    16501 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    16561 tcctttagca ccaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    16621 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    16681 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    16741 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    16801 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt tttatgtaaa
    16861 aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct
    16921 atacttttgt atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta
    16981 ataattaaaa ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt
    17041 tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca
    17101 ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg
    17161 actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag
    17221 ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta
    17281 acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat
    17341 ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa
    17401 tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt
    17461 atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat
    17521 gcacctgtcc aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct
    17581 gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta
    17641 gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag
    17701 acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa
    17761 gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa
    17821 cgaagtgatc tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg
    17881 aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct
    17941 tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca
    18001 ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa
    18061 gcaaaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct
    18121 ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat
    18181 gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg
    18241 ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca
    18301 ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt tttattctct
    18361 gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat
    18421 gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt
    18481 gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc
    18541 cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga
    18601 cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc
    18661 ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac
    18721 gggacattag gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata
    18781 ttagaaaaag gaactctggg attgggaaca ttgcatgggc ttgatggtga agtcatcttt
    18841 ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt
    18901 gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca
    18961 aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac
    19021 ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg
    19081 aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa
    19141 caaagcaata tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt
    19201 gctgcagcgg gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta
    19261 atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag
    19321 cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca
    19381 gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct
    19441 gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg agctataatc
    19501 ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat
    19561 gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta
    19621 gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca
    19681 ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct
    19741 tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca
    19801 ttgatcgttg atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa
    19861 tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat
    19921 tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat
    19981 cttttattag caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta
    20041 ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tggtcatttc
    20101 tgtttttcgg tttttttatt tttttataaa atcacaagtg ttattgattt tcacaatcat
    20161 attttaatgg tattatcatt ttgaataatg aaaaaacatt aaggatggat gaaatatgaa
    20221 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
    20281 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
    20341 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
    20401 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
    20461 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
    20521 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
    20581 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
    20641 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
    20701 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
    20761 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
    20821 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
    20881 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
    20941 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
    21001 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
    21061 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
    21121 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
    21181 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
    21241 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
    21301 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc
    21361 ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt
    21421 ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt
    21481 ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt
    21541 tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat
    21601 ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga
    21661 agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa
    21721 tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa
    21781 ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa cttatttgaa
    21841 ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt tcacctcaaa
    21901 aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc tgaatacgta
    21961 ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca aaaaattttg
    22021 tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa caacgttttt
    22081 catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc gaacccgcgt
    22141 ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag gattcacttt
    22201 acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt tttcaactta
    22261 cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag cacatgggcg
    22321 atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag ttgttttcgt
    22381 ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg aaacgcaatt
    22441 caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag ggttacgtgt
    22501 atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa aagcgaatca
    22561 tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg attttcttaa
    22621 gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca atgcgtggac
    22681 aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta atccgtcata
    22741 ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat cattcggacc
    22801 atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca gacgtttgac
    22861 acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac cattccgata
    22921 aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag ataagatcag
    22981 caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa catctgaatg
    23041 aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga ttttctgatc
    23101 gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt tttgacattc
    23161 ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga aagaaccatt
    23221 catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt ctgcttctgt
    23281 acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca tagcttttgc
    23341 cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat caaagaaagt
    23401 gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag tttatcttaa
    23461 caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt ggttcagtca
    23521 acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt tttttcagta
    23581 ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca aaaggaaatc
    23641 tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat tggcggctgg
    23701 ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca ttgatcggct
    23761 attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt ctgctagtat
    23821 ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat gacctggaaa
    23881 ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt ttctgaaatg
    23941 atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga tggaaaagaa
    24001 gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact agacctgaat
    24061 ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc aagtactgtg
    24121 ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg cttagagatg
    24181 ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc tcgaatgaaa
    24241 gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt ttcagatgat
    24301 cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga agcgtatctg
    24361 gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt tactttagca
    24421 aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg ggattatcag
    24481 tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca aaaaatcgaa
    24541 aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt cgatgattct
    24601 ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga atggtcgcgt
    24661 tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg attgaagaaa
    24721 acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg caaacctatg
    24781 ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg ctgctgacag
    24841 agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac gaacgtgtca
    24901 tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa tggaaaaatc
    24961 tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga caagcacagg
    25021 cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga gcacttgtag
    25081 ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg aacctgttca
    25141 aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt gcagcattcc
    25201 aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat ggtaattttg
    25261 atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat gatttcaata
    25321 aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa aatcgtcgaa
    25381 gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca atcaacggac
    25441 ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac gcgattaaat
    25501 tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg gattatgaaa
    25561 aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca gaaacattga
    25621 aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca ttgacattga
    25681 aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa atcatggtca
    25741 atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt actttagaaa
    25801 aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg tctgaacaac
    25861 aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa aatacaaaat
    25921 atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg cataaaggaa
    25981 aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt ttgcctgacg
    26041 tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg atcttctata
    26101 ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg atcaatataa
    26161 ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt ttcttcatga
    26221 gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag ttcatcaaat
    26281 gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt
    26341 actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg agagatatca
    26401 agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac atccaacagg
    26461 acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat gatcgcaaaa
    26521 ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt atgctgtttt
    26581 tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt
    26641 acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga atagactaag
    26701 aatgagacag catccactga gcccaataag agtttgggca aatcccttaa gtatatctgt
    26761 gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt
    26821 aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag tctttaagac
    26881 aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta cgatccacaa
    26941 taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa
    27001 taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct tgttttgcct
    27061 gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata aactgaatat
    27121 gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg tagatgcaga
    27181 agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt gcaaatggta
    27241 aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac aatagagtat
    27301 tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt tcatactttt
    27361 cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc tttagtgaaa
    27421 tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag aagctgccct
    27481 attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag gagcagcgac
    27541 aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac ttgtggtcgt
    27601 tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg
    27661 ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc
    27721 taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata
    27781 gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt
    27841 ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc
    27901 agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc
    27961 atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat
    28021 ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc
    28081 gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac
    28141 acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc
    28201 agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac
    28261 tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc
    28321 taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg
    28381 gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa
    28441 ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg
    28501 tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc
    28561 aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc
    28621 gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc
    28681 ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa
    28741 tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa
    28801 gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc
    28861 atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc
    28921 taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt catttggtgc
    28981 tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc
    29041 ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc
    29101 aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc
    29161 ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc
    29221 cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt
    29281 caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact
    29341 ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc
    29401 cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat
    29461 gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa
    29521 taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat
    29581 tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag
    29641 taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat
    29701 aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt acacagctca
    29761 cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat
    29821 gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta
    29881 tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt
    29941 aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct
    30001 tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt
    30061 tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca
    30121 gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag
    30181 aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga
    30241 cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa
    30301 cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga
    30361 aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt
    30421 catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc
    30481 cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg
    30541 atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg
    30601 cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc
    30661 aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca
    30721 gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt
    30781 tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt
    30841 tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga
    30901 cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat
    30961 gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct
    31021 gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt
    31081 ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg taaagggatt
    31141 gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga
    31201 agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt
    31261 cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca
    31321 tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga
    31381 acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt
    31441 agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga
    31501 ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc
    31561 agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg
    31621 aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag
    31681 acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa atcttggtaa
    31741 aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag
    31801 cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt
    31861 attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag
    31921 taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat
    31981 atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc
    32041 aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg
    32101 tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa
    32161 aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct
    32221 gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct
    32281 atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag
    32341 atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa
    32401 aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta
    32461 tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt
    32521 aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat
    32581 gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac
    32641 aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg
    32701 aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag
    32761 ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa
    32821 gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg
    32881 tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt
    32941 gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa
    33001 atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc
    33061 ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg
    33121 ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc
    33181 atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg
    33241 agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa
    33301 tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa
    33361 tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc
    33421 ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc
    33481 aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg
    33541 acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt
    33601 tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat
    33661 catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc
    33721 tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt tgagccaaaa
    33781 gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca
    33841 acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa
    33901 tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc
    33961 gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt
    34021 cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa
    34081 tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta
    34141 agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca
    34201 atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc
    34261 atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat
    34321 gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat
    34381 aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga
    34441 agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg
    34501 aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta
    34561 gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat
    34621 tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa
    34681 gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat
    34741 gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact
    34801 caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta
    34861 gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc
    34921 gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa
    34981 atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat
    35041 caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa
    35101 aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca
    35161 gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat
    35221 ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga
    35281 ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa
    35341 caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt
    35401 actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa
    35461 gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt
    35521 gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac
    35581 acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga
    35641 aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt
    35701 tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta
    35761 ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct
    35821 acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact
    35881 ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta
    35941 ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc
    36001 aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc
    36061 aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg
    36121 caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa
    36181 ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc
    36241 caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca
    36301 atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa
    36361 gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac
    36421 gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg
    36481 tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac
    36541 caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt
    36601 gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta
    36661 aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact
    36721 caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc
    36781 cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga
    36841 acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa
    36901 aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt
    36961 tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct
    37021 tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt
    37081 caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat
    37141 ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt
    37201 ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa gtagactgat
    37261 agtagtccca acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa
    37321 tttttttcat gaaagatctc cttttttatt tttattatac ccgtggtgct agttaatttt
    37381 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
    37441 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
    37501 tgaaattttg acaacctaat ttttctaagg aagaa
//
LOCUS       DASJPC010000019        34869 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJPC010000019 DASJPC010000000
VERSION     DASJPC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.019"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..187)
                     /locus_tag="U4A13_000926"
     CDS             complement(59..187)
                     /locus_tag="U4A13_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1779242.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="U4A13_000927"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="U4A13_000927"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1779243.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="U4A13_000928"
     CDS             complement(1674..2729)
                     /locus_tag="U4A13_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1779244.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="U4A13_000929"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="U4A13_000929"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN1779245.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="U4A13_000930"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="U4A13_000930"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN1779246.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="U4A13_000931"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="U4A13_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN1779247.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="U4A13_000932"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="U4A13_000932"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN1779248.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="U4A13_000933"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="U4A13_000933"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN1779249.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="U4A13_000934"
     CDS             10491..12050
                     /locus_tag="U4A13_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN1779250.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="U4A13_000935"
     CDS             complement(12161..14092)
                     /locus_tag="U4A13_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1779251.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="U4A13_000936"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="U4A13_000936"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1779252.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="U4A13_000937"
     CDS             complement(15021..15773)
                     /locus_tag="U4A13_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1779253.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="U4A13_000938"
     CDS             complement(15932..16141)
                     /locus_tag="U4A13_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779254.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="U4A13_000939"
     CDS             complement(16152..17480)
                     /locus_tag="U4A13_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779255.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="U4A13_000940"
     CDS             17616..17771
                     /locus_tag="U4A13_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779256.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="U4A13_000941"
     CDS             complement(17845..18432)
                     /locus_tag="U4A13_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN1779257.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="U4A13_000942"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="U4A13_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN1779258.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="U4A13_000943"
     CDS             complement(20012..21301)
                     /locus_tag="U4A13_000943"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN1779259.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="U4A13_000944"
     CDS             complement(21455..22387)
                     /locus_tag="U4A13_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779260.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="U4A13_000945"
     CDS             complement(22544..23203)
                     /locus_tag="U4A13_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN1779261.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="U4A13_000946"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="U4A13_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN1779262.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="U4A13_000947"
     CDS             complement(24244..24534)
                     /locus_tag="U4A13_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779263.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="U4A13_000948"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="U4A13_000948"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN1779264.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="U4A13_000949"
     CDS             complement(26820..28175)
                     /locus_tag="U4A13_000949"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN1779265.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="U4A13_000950"
     CDS             complement(28199..29368)
                     /locus_tag="U4A13_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779266.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="U4A13_000951"
     CDS             complement(29365..30252)
                     /locus_tag="U4A13_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN1779267.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="U4A13_000952"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="U4A13_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN1779268.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="U4A13_000953"
     CDS             34332..34853
                     /locus_tag="U4A13_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1779269.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         9400 a         7569 c         6284 g        11616 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtg
//
LOCUS       DASJPC010000020        34566 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJPC010000020 DASJPC010000000
VERSION     DASJPC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.020"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(258..1544)
                     /locus_tag="U4A13_000954"
     CDS             complement(258..1544)
                     /locus_tag="U4A13_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1779270.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1537..2805)
                     /locus_tag="U4A13_000955"
     CDS             complement(1537..2805)
                     /locus_tag="U4A13_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN1779271.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            3057..3518
                     /gene="mscL"
                     /locus_tag="U4A13_000956"
     CDS             3057..3518
                     /gene="mscL"
                     /locus_tag="U4A13_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN1779272.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3578..4024)
                     /locus_tag="U4A13_000957"
     CDS             complement(3578..4024)
                     /locus_tag="U4A13_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1779273.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4117..4362)
                     /locus_tag="U4A13_000958"
     CDS             complement(4117..4362)
                     /locus_tag="U4A13_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN1779274.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4477..5055)
                     /locus_tag="U4A13_000959"
     CDS             complement(4477..5055)
                     /locus_tag="U4A13_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1779275.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5132..5662)
                     /locus_tag="U4A13_000960"
     CDS             complement(5132..5662)
                     /locus_tag="U4A13_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN1779276.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5666..6526)
                     /gene="rlmB"
                     /locus_tag="U4A13_000961"
     CDS             complement(5666..6526)
                     /gene="rlmB"
                     /locus_tag="U4A13_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN1779277.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6519..6914)
                     /locus_tag="U4A13_000962"
     CDS             complement(6519..6914)
                     /locus_tag="U4A13_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN1779278.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(6911..8323)
                     /locus_tag="U4A13_000963"
     CDS             complement(6911..8323)
                     /locus_tag="U4A13_000963"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN1779279.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8320..8856)
                     /gene="cysE"
                     /locus_tag="U4A13_000964"
     CDS             complement(8320..8856)
                     /gene="cysE"
                     /locus_tag="U4A13_000964"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN1779280.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(8942..9181)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9178..10638)
                     /locus_tag="U4A13_000965"
     CDS             complement(9178..10638)
                     /locus_tag="U4A13_000965"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN1779281.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10808..11965)
                     /locus_tag="U4A13_000966"
     CDS             complement(10808..11965)
                     /locus_tag="U4A13_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN1779282.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(11999..13369)
                     /gene="radA"
                     /locus_tag="U4A13_000967"
     CDS             complement(11999..13369)
                     /gene="radA"
                     /locus_tag="U4A13_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN1779283.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13432..13959)
                     /locus_tag="U4A13_000968"
     CDS             complement(13432..13959)
                     /locus_tag="U4A13_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN1779284.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            14189..14995
                     /gene="proB"
                     /locus_tag="U4A13_000969"
     CDS             14189..14995
                     /gene="proB"
                     /locus_tag="U4A13_000969"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN1779285.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            14992..16239
                     /locus_tag="U4A13_000970"
     CDS             14992..16239
                     /locus_tag="U4A13_000970"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN1779286.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            16315..16659
                     /locus_tag="U4A13_000971"
     CDS             16315..16659
                     /locus_tag="U4A13_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779287.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(16776..17102)
                     /locus_tag="U4A13_000972"
     CDS             complement(16776..17102)
                     /locus_tag="U4A13_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779288.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(17717..18919)
                     /locus_tag="U4A13_000973"
     CDS             complement(17717..18919)
                     /locus_tag="U4A13_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1779289.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            19036..19551
                     /locus_tag="U4A13_000974"
     CDS             19036..19551
                     /locus_tag="U4A13_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1779290.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(19750..20196)
                     /locus_tag="U4A13_000975"
     CDS             complement(19750..20196)
                     /locus_tag="U4A13_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1779291.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(20243..21721)
                     /gene="msr(C)"
                     /locus_tag="U4A13_000976"
     CDS             complement(20243..21721)
                     /gene="msr(C)"
                     /locus_tag="U4A13_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1779292.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(22075..22704)
                     /locus_tag="U4A13_000977"
     CDS             complement(22075..22704)
                     /locus_tag="U4A13_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1779293.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(22688..23152)
                     /locus_tag="U4A13_000978"
     CDS             complement(22688..23152)
                     /locus_tag="U4A13_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1779294.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(23639..24907)
                     /locus_tag="U4A13_000979"
     CDS             complement(23639..24907)
                     /locus_tag="U4A13_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN1779295.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(25138..25875)
                     /locus_tag="U4A13_000980"
     CDS             complement(25138..25875)
                     /locus_tag="U4A13_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1779296.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(25942..26511)
                     /locus_tag="U4A13_000981"
     CDS             complement(25942..26511)
                     /locus_tag="U4A13_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN1779297.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            26639..27511
                     /locus_tag="U4A13_000982"
     CDS             26639..27511
                     /locus_tag="U4A13_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1779298.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            27853..28725
                     /locus_tag="U4A13_000983"
     CDS             27853..28725
                     /locus_tag="U4A13_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1779299.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            28722..30680
                     /locus_tag="U4A13_000984"
     CDS             28722..30680
                     /locus_tag="U4A13_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1779300.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(31012..31863)
                     /locus_tag="U4A13_000985"
     CDS             complement(31012..31863)
                     /locus_tag="U4A13_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1779301.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            31986..32453
                     /locus_tag="U4A13_000986"
     CDS             31986..32453
                     /locus_tag="U4A13_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN1779302.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(32716..33198)
                     /locus_tag="U4A13_000987"
     CDS             complement(32716..33198)
                     /locus_tag="U4A13_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN1779303.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(33298..34161)
                     /locus_tag="U4A13_000988"
     CDS             complement(33298..34161)
                     /locus_tag="U4A13_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1779304.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         9873 a         7279 c         6223 g        11191 t
ORIGIN      
        1 aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
       61 ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata taagttgatt
      121 aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac taagaatacc
      181 ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt taagactttt
      241 cctcataaaa tactggttta ttttggtctc atgtaaaact cgctgaaggc ctcttcactt
      301 ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat gatttcagga
      361 agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc aatatattcg
      421 atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa atcgagattt
      481 gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac tttttctgct
      541 gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc acgatccaag
      601 ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc tagatagttt
      661 cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt atagcgtaac
      721 agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac aaatttatct
      781 cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa
      841 taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc ccgaataagt
      901 ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt gcttggctga
      961 taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat actttcaacc
     1021 gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag tatgccaaaa
     1081 aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat gattcctttt
     1141 tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc
     1201 agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg
     1261 aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc atcttctttt
     1321 tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac cttttctttt
     1381 tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa caaaccatat
     1441 gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg cagtacttca
     1501 tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat ttcctcctgc
     1561 taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct ctttgatcgt
     1621 tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg gcagccagct
     1681 gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca gatattggtt
     1741 tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat ttcgcaaact
     1801 ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat cgataccggt
     1861 ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat aagctagact
     1921 ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt
     1981 aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct gataagctag
     2041 gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga tattcacttg
     2101 aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg gtaattgccc
     2161 aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt cgatttgatc
     2221 atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct tgtccaaatc
     2281 ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat aaagttcttg
     2341 caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat atagacgcag
     2401 attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa tatttggata
     2461 aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat cattgacata
     2521 ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt tttttctaac
     2581 tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact ttgtctgatc
     2641 cgaataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt tcgtgatgac
     2701 ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt attgttttgt
     2761 tggtaagaca tgaagattca cgccttctgc caattgatac accatattta tatgctcctt
     2821 ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt tacttattcc
     2881 tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat gttacacttt
     2941 atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg tatattgttt
     3001 ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg tcattcatga
     3061 tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg
     3121 ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg atcacaccac
     3181 taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga gcactttcaa
     3241 ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt attagtgcta
     3301 tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg
     3361 cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct actgcagaag
     3421 attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac aatacacatc
     3481 cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa aaaaaaagaa
     3541 agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt
     3601 tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa
     3661 acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa
     3721 aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac
     3781 ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc
     3841 gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat
     3901 caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt
     3961 ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt
     4021 ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag
     4081 caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc
     4141 gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt
     4201 ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg
     4261 gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg
     4321 accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat
     4381 caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat
     4441 aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa
     4501 gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat
     4561 tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt
     4621 agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg
     4681 ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc
     4741 tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt
     4801 cctcaacaga gaatagattt gattgcttat aacgctttta taataacttc caaagctaac
     4861 acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc
     4921 ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg
     4981 aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa
     5041 taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc
     5101 aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca
     5161 atttttggga caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa
     5221 taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat
     5281 ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta
     5341 aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct
     5401 cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct
     5461 tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc
     5521 cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt
     5581 tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta
     5641 caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca
     5701 taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg
     5761 tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa
     5821 tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg
     5881 tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca
     5941 cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga
     6001 ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc
     6061 gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt
     6121 cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg
     6181 taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt
     6241 taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt
     6301 tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt
     6361 ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag
     6421 aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt
     6481 caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact
     6541 ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt caaatgcaga
     6601 tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc agcatttttt
     6661 gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc ttcttctgtc
     6721 aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa
     6781 ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat caggtggtca
     6841 cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc gttcaattgt
     6901 gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct tctaataaga
     6961 tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc
     7021 ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac tcttctgctg
     7081 taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct tgtgccaaga
     7141 cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca agctcataga
     7201 ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg aatcgctgtt
     7261 ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct gaggcagttt
     7321 cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt
     7381 ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc gccatgaaga
     7441 aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg aaattcccta
     7501 gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg tgcatccagt
     7561 aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt tcatgatgag
     7621 gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg
     7681 ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt gaatcccaag
     7741 aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct tctttttgct
     7801 gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg gattggtcac
     7861 ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct gaagattcat
     7921 aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc atatgatcca
     7981 tgactcttgg atgcagtgta gcaggttcga cattcaacgc tttcgtatct tcttcaaatg
     8041 ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt gctgtacgga
     8101 tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag cctcgatatt
     8161 caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg atatggatat
     8221 aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt atagggatga
     8281 attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc tcctcacttt
     8341 ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt cgtctggaac
     8401 atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc
     8461 taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt gtcttttacc
     8521 cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat cgtcctcgat
     8581 ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc cagttccaat
     8641 cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag cattgatctt
     8701 cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc aaaacaaggc
     8761 atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct
     8821 ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct ttcgtaagaa
     8881 ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat aagtttcctt
     8941 atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt atctctgaca
     9001 gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt tttcctgttt
     9061 tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt gataccttgc
     9121 accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc atcgtgttta
     9181 gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt cgatcgtttc
     9241 aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca tgaaaagatt
     9301 ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga cagttggagc
     9361 atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag taggaactgt
     9421 ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat tgaaaaatag
     9481 cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca ggctaacgat
     9541 cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc catcagcgat
     9601 tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga tgtaatggtt
     9661 gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta ggcgttttgg
     9721 atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca caggtgacca
     9781 gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt cgcgatattg
     9841 ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg tttcactgtt
     9901 gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt catagatcat
     9961 cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag tgattttcat
    10021 caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc gtttttggat
    10081 gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca ccatatctgt
    10141 aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta gaccttgtgc
    10201 ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat
    10261 ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg tgcaatagca
    10321 tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga tttctttacg
    10381 ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat cccaattcat
    10441 tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat tacgtttttg
    10501 gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa acaaataatt
    10561 gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg gtgcatagcg
    10621 tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac taacagcaaa
    10681 aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag cacaaatttt
    10741 accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag ttggctgaat
    10801 ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg tcgattcctc
    10861 gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga agggcacttg
    10921 ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg acaaccatcg
    10981 tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt actactagaa
    11041 cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac gcattgatat
    11101 taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca ttggttacaa
    11161 tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca ctgatatctt
    11221 caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag gcatttagga
    11281 tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt
    11341 gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat ccagtctttg
    11401 taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta tatttatgga
    11461 aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct gtttcttgct
    11521 cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt gtcgttccca
    11581 ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca ctattgacga
    11641 atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc aatccgatga
    11701 tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt ttttgttcta
    11761 cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa aagaaaataa
    11821 gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac tgttgaccga
    11881 ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca atgatcatca
    11941 gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca tttattagtt
    12001 agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc tcgattcctt
    12061 ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg aagccgagtt
    12121 tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct ccagtcagtc
    12181 caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag cttgaagcaa
    12241 tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca cctgcagctt
    12301 ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc actgccatga
    12361 tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt ccgaacatag
    12421 taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc attgccacaa
    12481 cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt gaaggattag
    12541 cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc gttgagccaa
    12601 atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct tcaaaatata
    12661 acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct tctttggtaa
    12721 catgaccgac gataaaaatg gctatcccat ttgttttagc ggttttgagt agttctgcag
    12781 tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc tgcgtcatcg
    12841 tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca cgactgattt
    12901 cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg aggcgttccg
    12961 cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact tttccgccta
    13021 tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata cctggatcgc
    13081 caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg ttcagttctt
    13141 ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt tttgttggtt
    13201 ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct tgttcgattt
    13261 cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca
    13321 catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt gtaccgcctc
    13381 cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt tttatttact
    13441 ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg cttttaaaaa
    13501 cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat ccttgaaacc
    13561 aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt aataatcact
    13621 gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg
    13681 gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag taggcacgag
    13741 ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat ctgcgactgt
    13801 ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct cttctgctgc
    13861 ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga tattttgatc
    13921 tttatattta gaaataactt caaatcctct ttttctcacc ggttttcctc cctaaataaa
    13981 ctactcctat tctaacatat ccgaggctgt aaaagacttt ctttacaaat tcttcaagaa
    14041 attctgctat cttaaagtaa accattaatt cgttcttttt ccaaacgact gcaagttgta
    14101 caaaaaaact attttttaca tgactttact cgtcaaaaca agtagaatag aacgaaatta
    14161 atgagtggat atttgattga ggtgtcacat gcgtaaagaa attaaaaatg caaaacgaat
    14221 tattgtcaaa gtaggaacaa gtacattgat ttatcccaat ggaaatatca atcttgcggc
    14281 aatcgatcag ttagcttttg tcctttctga tcttagaaat cggggaaagg aagtgatctt
    14341 agtctcatca ggagctgtag gcgttggtat gcataaatta catttgtcag aacgtccgac
    14401 tttgattcct gaacagcaag ctgcggcggc tgttggtcaa gccgaactga tgaatatcta
    14461 taatcaaaga ttcagtgtat ataatcaaca gacggcacag atcctcgtga caagagacgt
    14521 ggtagattat cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc ttttgattat
    14581 ggacacgatc cctatcgtga acgaaaacga tactgtagca gtggatgaat tagatcatct
    14641 gacaaaattt ggcgataatg atcagctgtc agccatcatg gcaaaacttg tcgcggcaga
    14701 ccttttgatc gtcctttctg atatcgacgg gttttattca gataatccga ctacaaatcc
    14761 cgaggctcag atgtatcgct atatcgataa aatcgatgaa aagattctgg cacaagcagg
    14821 tggtgctggt agcacatttg gaacaggcgg tatgcagagt aaattaaaag ccgcttctag
    14881 gatatttgaa gcaaacagcg caatgatttt agcaaatgga aaaaatccgg caattatttt
    14941 tgatattctt gcaggaaaag aagtaggtac tttatttaag gaggacttga aatgactgac
    15001 ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac aactagcact tatgcctaca
    15061 aaccgcaaaa atgagctact tgttcaaatg gcacaaacca tcaaaaagaa tcaacaagcg
    15121 atcattgaag caaatcataa agacttagaa aaagcagtcg aaaacaatat cagtgaaacg
    15181 atgcaagacc gcttgcagct gacacctgag cgaatcaatg ccatggctac agagatcgaa
    15241 aaaattgctt cgttagacga tcctattgga aaagtagatg agatgtggac gaacaccgac
    15301 ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat ttacgaatct
    15361 cgtccaaatg tcacgacaga tgcagctagt ctagcattca aatcagggaa tgccgttatc
    15421 cttcgcggtg gcaaagaagc attcttctcg aatcaattat tagtccaatt attgcagcaa
    15481 gtactggtca gtgctggcga atctccttat gccatccagt ttgtcgatga tacttctcat
    15541 gaaactgcag gacagttgat gcgattgaca gaatacttag atgtgctgat tcctcgaggc
    15601 ggagcaaaat taatccaacg agtcaaagaa caggcaacgg tacctatcat tgaaacaggt
    15661 acaggaaata atcacgtata tatcgataaa gaagcacagt tatctatggc agtgaacatc
    15721 atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg cggcagaaac attgctgatc
    15781 cattctgaga tcgcaccttt tttcttgcca gctattgaaa aagaactggt agaacacggg
    15841 gtctctttac gtgcggatgc tcgtgcgtta gaatatctag agacggctgc tttagcagaa
    15901 gacacagatt gggatactga atatctagat tatatccttg ctgtcaaagt ggtcgattct
    15961 ttggatgaag caattgcaca tatcaatcgg cataatacta aacattcaga aacgatcgtg
    16021 acagatagtt atgcggctag tcaacgtttc ttaaatgaag tggacgcggc ggctgtctat
    16081 gtcaatgcct ccactcgttt tacagacgga tcggtatttg gttttggtgc agagattggt
    16141 atctctactc aaaagttgca tgcccgtggt ccaatggggt taaatgaatt aacttccaca
    16201 aaatatatca tttatggcga tggacaaatc cgcgaataat gtttcgtgtg aaacattatt
    16261 cccgcctttt gccttttttc tgtaaaggtt ctatccaact agaaaggtgt caatatgttt
    16321 gaaattttaa tgattttttt tatttacctt atttctctga atatcgctgc ttttctggga
    16381 gtcggtattt tatccctttt ctttcaattc aaaaaacgaa gcatagggag tcaacgggaa
    16441 aaatggtcgc agtattttga caaaatcggg caaaaagggc tggttacacg cttgcacatt
    16501 agttacatgg ttgcattatg cttattagcg acaatcaatt actattcttt tttcgatcat
    16561 tcaatcgctt atacgattac gctactgatt gctgggattt ttcacttatc ttataaatat
    16621 cagctgaata aaaaccactt gaatcggact ttccgctaaa aaatatccgt acgataagta
    16681 gagtgccttg aagatcggca aaaaactgcc ggacttttta gaggctgtga caaagatttt
    16741 gtcacagcct ctttttccat ataaacggcg ttcgcctacc agctgcctgg taaatctgtt
    16801 tcctcttcgt taagaagcga aaatccgtca aatgtttcgc catcataata acgaagcagc
    16861 tcatccatgg acaagttcat cgttttttct ttttcttctt ccgctgattc agccagtagc
    16921 tgttcccgat acacctgata cataccagct attttttgct gcatcgcttt tctagtttca
    16981 ccttccgcat tgattattgg caattctttg atatagccac gataaatcaa ccgatcatct
    17041 gaaaattcaa tgggtaattc aacgagtgtc aacccgactt cctgaaaata ttcttccagc
    17101 atctattatc cccccattct ttttcttaaa tgaattttag cttacctact gaaaattttc
    17161 cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac atcccctttt
    17221 tccgaataaa tggtgttctt ctgccaactc ctcaatcata agtcacaata ttcaacaaac
    17281 atgagaaact gttttttgaa attgttcctt gtgattcaga gttaaacgac agtctcacaa
    17341 cctcttggta aacggcgttc aatcagctga ccctcggtat tagctcaaaa aacggtaaaa
    17401 tatgagaagc tatttttccg ttttgtttct ctaatactta ggataaaacg ctgattttac
    17461 aacctctgtt aaacggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa
    17521 atatgaggaa ctatttttcc gtttgtttct ctaatactca ggacaaaacg ctgattttac
    17581 aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    17641 aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    17701 cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga
    17761 tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa
    17821 tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa
    17881 aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg
    17941 tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa
    18001 ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg
    18061 ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg
    18121 aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat
    18181 agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca
    18241 atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa
    18301 ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac
    18361 cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga
    18421 ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga
    18481 ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa
    18541 ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag
    18601 ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt
    18661 tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca
    18721 tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg
    18781 tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta
    18841 caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt
    18901 gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt tattttacat
    18961 gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg
    19021 agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa
    19081 ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt
    19141 cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa
    19201 gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag
    19261 gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc
    19321 tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa
    19381 cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag
    19441 aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa
    19501 ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac
    19561 agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag
    19621 ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt
    19681 tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac
    19741 agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac
    19801 gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc
    19861 agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt
    19921 caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg
    19981 atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa
    20041 aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc
    20101 ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt
    20161 tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt
    20221 cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc
    20281 cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa
    20341 acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat
    20401 caataaaatt agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag
    20461 caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt
    20521 cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt
    20581 gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat
    20641 atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt
    20701 ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag
    20761 aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt
    20821 gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt
    20881 gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt
    20941 tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat
    21001 ccagtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt
    21061 ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt
    21121 ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg
    21181 aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa
    21241 aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca
    21301 gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt
    21361 cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc
    21421 gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct
    21481 ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt
    21541 tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac
    21601 cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata
    21661 gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca
    21721 tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa
    21781 aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga
    21841 ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca
    21901 tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca
    21961 aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt
    22021 taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc
    22081 gaacatagtg atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt
    22141 tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc
    22201 caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat cacgcgcaac
    22261 taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc
    22321 tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt
    22381 tggcatcttc cctgattcgt aagcttggtt gtagatttct tcgccatatt cgtaacattg
    22441 catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg
    22501 gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg
    22561 tttcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc
    22621 gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt
    22681 tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc
    22741 aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga
    22801 aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc ttgattttct
    22861 tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac
    22921 gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa
    22981 tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca
    23041 tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt
    23101 gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct
    23161 cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa
    23221 aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa
    23281 gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga
    23341 ttattgattc ttatcaaact atagaagata gctcttctgc agtagataat tttacaccgc
    23401 caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt
    23461 atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa
    23521 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
    23581 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
    23641 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
    23701 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
    23761 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
    23821 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
    23881 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
    23941 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
    24001 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
    24061 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
    24121 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
    24181 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
    24241 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
    24301 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
    24361 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
    24421 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
    24481 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
    24541 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
    24601 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
    24661 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
    24721 aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg ttttcaattc
    24781 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
    24841 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
    24901 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
    24961 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta ataaatttgc
    25021 tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
    25081 tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg ttatggatta
    25141 tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt ctggcgtttc
    25201 tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt ctttttgcgc
    25261 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
    25321 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
    25381 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac
    25441 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
    25501 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
    25561 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
    25621 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
    25681 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
    25741 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
    25801 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
    25861 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
    25921 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
    25981 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
    26041 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
    26101 atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag
    26161 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
    26221 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
    26281 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
    26341 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
    26401 cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa agagcggatc
    26461 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
    26521 tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat aaaacatact
    26581 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
    26641 ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc
    26701 agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga
    26761 agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga cggattctgg
    26821 tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa
    26881 tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc
    26941 aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt
    27001 tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga
    27061 ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc
    27121 ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc aaacagtcat
    27181 ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag
    27241 tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa
    27301 cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga
    27361 tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc cgccattaca
    27421 agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt
    27481 tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt
    27541 tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag tttcttaatt
    27601 tgttccttcc ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt
    27661 gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac
    27721 tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt
    27781 gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa
    27841 aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac
    27901 ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat
    27961 ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag
    28021 ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt
    28081 gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc
    28141 gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt
    28201 ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag
    28261 tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc
    28321 catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg
    28381 attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa
    28441 ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc
    28501 catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac
    28561 ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc
    28621 gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca
    28681 atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac
    28741 gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag
    28801 ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg
    28861 gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga
    28921 tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag
    28981 taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg
    29041 atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg
    29101 cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg
    29161 gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac
    29221 tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt
    29281 taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat
    29341 tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta
    29401 aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc
    29461 caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag
    29521 aaaacaaagc aaaaacagct tccttgatgt tagcagcagc ttttgcttca ttctttactg
    29581 gtgtaacaga accacttgaa ttctcattta tgttcgttgc ttggccatta tatgtcttgc
    29641 atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat
    29701 ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc
    29761 agccattgat gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta
    29821 actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag
    29881 atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct
    29941 acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc
    30001 gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac
    30061 ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat
    30121 tcgttgcaga tgagatggca aaactacatg gtggtgctgc aacacgacct gcacaaacaa
    30181 acaccgtggt taaaacagaa acatttgccg gtggaacgat cgaacaagat atttacgcta
    30241 tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa
    30301 tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg
    30361 gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta
    30421 tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt
    30481 tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg
    30541 ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca
    30601 aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct
    30661 ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca
    30721 ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg
    30781 ccaactccgc ggtaataagt ccagccaaac gtaacatatg aagagctatt ttttatttga
    30841 ctcttcttat tacttgatgc agaacagctt ttttacaacc tctgataaac ggtgttcaaa
    30901 aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa
    30961 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
    31021 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
    31081 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
    31141 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
    31201 acttgcttca ataatttcta actttttacc tgtcgcacgc tctaatgcct tggctaattc
    31261 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
    31321 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
    31381 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
    31441 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
    31501 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
    31561 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
    31621 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
    31681 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
    31741 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
    31801 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
    31861 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
    31921 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
    31981 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
    32041 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
    32101 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
    32161 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
    32221 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
    32281 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
    32341 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
    32401 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
    32461 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    32521 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    32581 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    32641 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    32701 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    32761 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    32821 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    32881 cgattctgct aaagcgatta caacaagata cgttcctttt gggaatggga tgattcgggt
    32941 agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    33001 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    33061 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    33121 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    33181 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    33241 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    33301 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    33361 ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag gaagccattc
    33421 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    33481 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    33541 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc gggctccatc
    33601 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    33661 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    33721 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    33781 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    33841 tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat cacgaaaagc
    33901 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    33961 tccatctagg agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    34021 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    34081 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    34141 ttggttcaga tctttaagca tatttatctt cctccttgct tgccttttta tcatattatc
    34201 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    34261 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    34321 atctatgttt tacctttaga aaaataaatt ttggggaaga cacattctaa gtaaacaaag
    34381 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    34441 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    34501 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    34561 aataaa
//
LOCUS       DASJPC010000021        34330 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJPC010000021 DASJPC010000000
VERSION     DASJPC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.021"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            202..1077
                     /locus_tag="U4A13_000989"
     CDS             202..1077
                     /locus_tag="U4A13_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779305.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="U4A13_000990"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="U4A13_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="U4A13_000991"
     CDS             complement(1642..2973)
                     /locus_tag="U4A13_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1779306.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="U4A13_000992"
     CDS             complement(3122..3835)
                     /locus_tag="U4A13_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1779307.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="U4A13_000993"
     CDS             4010..5434
                     /locus_tag="U4A13_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1779308.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="U4A13_000994"
     CDS             5733..6440
                     /locus_tag="U4A13_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1779309.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="U4A13_000995"
     CDS             6427..6750
                     /locus_tag="U4A13_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1779310.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="U4A13_000996"
     CDS             complement(6839..7876)
                     /locus_tag="U4A13_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1779311.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="U4A13_000997"
     CDS             complement(8197..9180)
                     /locus_tag="U4A13_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN1779312.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="U4A13_000998"
     CDS             complement(9595..10365)
                     /locus_tag="U4A13_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779313.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="U4A13_000999"
     CDS             complement(10358..11293)
                     /locus_tag="U4A13_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779314.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="U4A13_001000"
     CDS             complement(11609..13258)
                     /locus_tag="U4A13_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1779315.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="U4A13_001001"
     CDS             complement(13375..15978)
                     /locus_tag="U4A13_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1779316.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="U4A13_001002"
     CDS             complement(15990..17039)
                     /locus_tag="U4A13_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN1779317.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="U4A13_001003"
     CDS             complement(17065..17379)
                     /locus_tag="U4A13_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN1779318.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="U4A13_001004"
     CDS             complement(17395..17841)
                     /locus_tag="U4A13_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1779319.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="U4A13_001005"
     CDS             complement(17842..19764)
                     /locus_tag="U4A13_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN1779320.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="U4A13_001006"
     CDS             complement(19976..20872)
                     /locus_tag="U4A13_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1779321.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="U4A13_001007"
     CDS             complement(20865..21545)
                     /locus_tag="U4A13_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1779322.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="U4A13_001008"
     CDS             complement(21546..22328)
                     /locus_tag="U4A13_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779323.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="U4A13_001009"
     CDS             complement(22321..23238)
                     /locus_tag="U4A13_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1779324.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="U4A13_001010"
     CDS             complement(23402..23725)
                     /locus_tag="U4A13_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779325.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="U4A13_001011"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="U4A13_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN1779326.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="U4A13_001012"
     CDS             27853..28167
                     /locus_tag="U4A13_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1779327.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="U4A13_001013"
     CDS             28180..28554
                     /locus_tag="U4A13_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1779328.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="U4A13_001014"
     CDS             28564..28899
                     /locus_tag="U4A13_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN1779329.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="U4A13_001015"
     CDS             28982..30322
                     /locus_tag="U4A13_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1779330.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="U4A13_001016"
     CDS             30400..31074
                     /locus_tag="U4A13_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN1779331.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="U4A13_001017"
     CDS             31067..31231
                     /locus_tag="U4A13_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779332.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="U4A13_001018"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="U4A13_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="U4A13_001019"
     CDS             32439..32729
                     /locus_tag="U4A13_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779333.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="U4A13_001020"
     CDS             32988..34172
                     /locus_tag="U4A13_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1779334.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="U4A13_001021"
     CDS             34169..34306
                     /locus_tag="U4A13_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1779335.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DASJPC010000022        34004 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJPC010000022 DASJPC010000000
VERSION     DASJPC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.022"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1107
                     /locus_tag="U4A13_001022"
     CDS             <1..1107
                     /locus_tag="U4A13_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1779336.1"
                     /translation="AIKAENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRY
                     ELGQTIRSNRPPSDITISNTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEY
                     ANIKIIKGYVRSMFDIAEILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSK
                     ELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKH
                     TLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTK
                     PSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNAL
                     THSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
     gene            complement(1195..1587)
                     /gene="rpsI"
                     /locus_tag="U4A13_001023"
     CDS             complement(1195..1587)
                     /gene="rpsI"
                     /locus_tag="U4A13_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN1779337.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1603..2046)
                     /gene="rplM"
                     /locus_tag="U4A13_001024"
     CDS             complement(1603..2046)
                     /gene="rplM"
                     /locus_tag="U4A13_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN1779338.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(2565..3032)
                     /locus_tag="U4A13_001025"
     CDS             complement(2565..3032)
                     /locus_tag="U4A13_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN1779339.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(3152..4690)
                     /locus_tag="U4A13_001026"
     CDS             complement(3152..4690)
                     /locus_tag="U4A13_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN1779340.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            4770..5456
                     /locus_tag="U4A13_001027"
     CDS             4770..5456
                     /locus_tag="U4A13_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN1779341.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(5657..6184)
                     /gene="msrA"
                     /locus_tag="U4A13_001028"
     CDS             complement(5657..6184)
                     /gene="msrA"
                     /locus_tag="U4A13_001028"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1779342.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(6268..6945)
                     /gene="pgmB"
                     /locus_tag="U4A13_001029"
     CDS             complement(6268..6945)
                     /gene="pgmB"
                     /locus_tag="U4A13_001029"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN1779343.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(6942..8072)
                     /locus_tag="U4A13_001030"
     CDS             complement(6942..8072)
                     /locus_tag="U4A13_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1779344.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(8072..10255)
                     /locus_tag="U4A13_001031"
     CDS             complement(8072..10255)
                     /locus_tag="U4A13_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN1779345.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(10252..11277)
                     /locus_tag="U4A13_001032"
     CDS             complement(10252..11277)
                     /locus_tag="U4A13_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1779346.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(11322..12149)
                     /locus_tag="U4A13_001033"
     CDS             complement(11322..12149)
                     /locus_tag="U4A13_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1779347.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(12146..13213)
                     /locus_tag="U4A13_001034"
     CDS             complement(12146..13213)
                     /locus_tag="U4A13_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN1779348.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(13239..14075)
                     /locus_tag="U4A13_001035"
     CDS             complement(13239..14075)
                     /locus_tag="U4A13_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1779349.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(14072..14941)
                     /locus_tag="U4A13_001036"
     CDS             complement(14072..14941)
                     /locus_tag="U4A13_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1779350.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(15011..16294)
                     /locus_tag="U4A13_001037"
     CDS             complement(15011..16294)
                     /locus_tag="U4A13_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1779351.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(16308..17993)
                     /locus_tag="U4A13_001038"
     CDS             complement(16308..17993)
                     /locus_tag="U4A13_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN1779352.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            18268..19293
                     /locus_tag="U4A13_001039"
     CDS             18268..19293
                     /locus_tag="U4A13_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1779353.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(19888..23562)
                     /gene="rpoC"
                     /locus_tag="U4A13_001040"
     CDS             complement(19888..23562)
                     /gene="rpoC"
                     /locus_tag="U4A13_001040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN1779354.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(23620..27237)
                     /gene="rpoB"
                     /locus_tag="U4A13_001041"
     CDS             complement(23620..27237)
                     /gene="rpoB"
                     /locus_tag="U4A13_001041"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1779355.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            27965..28933
                     /locus_tag="U4A13_001042"
     CDS             27965..28933
                     /locus_tag="U4A13_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1779356.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            28951..29856
                     /locus_tag="U4A13_001043"
     CDS             28951..29856
                     /locus_tag="U4A13_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779357.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            29853..30659
                     /locus_tag="U4A13_001044"
     CDS             29853..30659
                     /locus_tag="U4A13_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1779358.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            30795..31784
                     /locus_tag="U4A13_001045"
     CDS             30795..31784
                     /locus_tag="U4A13_001045"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN1779359.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(32101..32625)
                     /locus_tag="U4A13_001046"
     CDS             complement(32101..32625)
                     /locus_tag="U4A13_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN1779360.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            32912..>34004
                     /gene="scm"
                     /locus_tag="U4A13_001047"
     CDS             32912..>34004
                     /gene="scm"
                     /locus_tag="U4A13_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1779361.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTT"
BASE COUNT         9681 a         7238 c         6373 g        10712 t
ORIGIN      
        1 gcgattaaag ccgaaaatga tatgaagaag aaaatagaaa aagttttgag agaggagaat
       61 gcaaactcat tagaattaaa aggaaaaata acgtttaaaa aattttacga atctaaatgg
      121 cttcctagat atgaattagg tcaaacaatt agatcaaaca ggcctcctag tgatataacc
      181 attagtaata caaaagatat atttcggttg catattttac ctatgtttgg tgaatacgca
      241 atgaattatc taaatatcaa tactgagata atatcagatg aattaaccaa aaaatcgaaa
      301 gaatatgcaa atataaaaat cataaaagga tatgttcgtt ccatgttcga tattgcagaa
      361 atactaaatt atatcgaatt taatcgaact actaaaatta ttcaaagtat cactgcacct
      421 aaaaaaaatg ctttagaaga aaaaagaata caagaaggta agcaagcact atcatcaaaa
      481 gaacttacta attggataga agcagtaaat gatgatttta ataatcattt acttactttt
      541 catgattaca cactttttat gattaccctg tatcttggtg atagaaaaag tgagacttac
      601 gctttgcaat ggaaatatat tgattttgaa aaacaaacgg tccgtcttaa acatacttta
      661 gataaatatc aaagaaaaaa gtttacaaaa ggtcgaaaag ataccgtcat tcaagtacca
      721 gaagttgtaa tgacattgct aagtgaatgg aaatcggtac aagctgatca attgttgaaa
      781 ttaaaaatta agcaaacgtt agatcagtat ctctttactt atactaaacc atctggagaa
      841 gtaaattgtc ctgttcatgc cgattaccta aactatcgaa tcaattcaat taaaagaagg
      901 catcctgact tagttcattt atctccacac aaactccgtc atacttatgc aactatagct
      961 agacaaggtg gagcagatat gaatcaaatt tcaaatgctt taacacactc agatatttct
     1021 acaacaaaga tatatgtgaa cacaccagat atagttgata aggctgtttt tgaagctttt
     1081 cagagaggat tgaataagtg tgattgatat gtgttcttaa atgtgttctt aaaaacaaaa
     1141 gaagcacttt ccaaataatt gaaaagtgcc agaaacgttg atatataaag gatattaacg
     1201 ttttgagaat tgtgaagctt tacgtgcttt cttaagacct ggttttttac gttcgaccat
     1261 acgtgcgtca cgagtaagta agccagcacg tttaagagct aaacggaagt caggatcaac
     1321 ttctagcaat gcacgagcga taccatgacg gatagctcct gattgtccag cgtatcctcc
     1381 gccgtttacg ttaacaaata cgtcatatgc gccttttgtt tcagtaacag cgaaaggttg
     1441 attgattact tcacgcaagt cagcatgtgg aatgtattct tcaacatctt ttttatttac
     1501 agtaattttg ccagttcctg gtactagacg tacgcgagca actgcgtttt tacgacggcc
     1561 tgtgccgata tattgaactt gtgccaatga aattccctcc tattagatta agtttgtgat
     1621 gtctaaaact tctggttgtt gtgctgcatg tggatgttct gctccaccgt atacgttcaa
     1681 ttttgtaaat tgtttgcggc ctaaagtgtt tttaggaagc atacctttta cagaagtttc
     1741 gatcaaacga cgagaatttt tagcacgcaa ttcgccagca gtgattgatt tcaatcctcc
     1801 tgggtagttg ctgtgacggt aataaatttt gtcagtcgct tttttacctg ttaatttcac
     1861 tttgtctgca ttgatgacga tgacgaaatc gccagtatcc aaatgaggtg tgaaagttgg
     1921 tttattttta ccacgtagta cagatgcaac aactgttgaa agacgtccca atggaacatc
     1981 agttgcgtct actacatacc atttacgttc tacttcgcct ggtttggcca tatatgttgt
     2041 acgcacgtta ttttcctcca atttaagttc tgatgtttga catacacttc gagtttccgg
     2101 ggctctttgt ggggcaaaca ataccattta acatattacc cgctaatcta gtcaatgtca
     2161 acgtttttct gcttgattga aaaatttata tgaaacttta cttcttttct ttcttttata
     2221 taaaagggtc gtttcctgtc aaaatgaaaa acaaaaagag gctgtgacaa aagtcttgtc
     2281 acaacccctt attttgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
     2341 tatccataaa acataagaag ctgtttttta aaattgtttc ttgtgactcg gagctgaacg
     2401 gcaggcttac aacctcttgg taaacggtgt tcaatcagct gaccctcggt attagctcaa
     2461 aaaacggcaa aatatgagga actattttga cgtttgtttc tctaatactc aggtcaaaac
     2521 gctgatttca caacctcttt ttcggtcaag ttcaacaaca gtttttacgc gtcgatctct
     2581 ggcgctttgc ccaaatgagc ttggatcatc cagatacgtt tttctgtggc tgttttgaat
     2641 tcgataaaga tatcttgtgt tggatcgtcg ccttcttctc cagaaacttc gattcctttt
     2701 tggtaaagat ctgctagata acgatagcct gcaacaagtg tttccaaacg ttctggaata
     2761 gtacgatccc agcggccaat ttcatcaggg atttttgtgt tatctgcaaa ttcacgcaat
     2821 gtagaataag gtgaaccgtc taatgtgatc aagcgttcgg atacttcatc taactgagcg
     2881 ttcaaatcat ccatgaactc atccatcatt gggtgcaatg taaggaaacc tggtcctctc
     2941 atgtaccaat gtgtttggtg aacaacaaca gacatttggc ttagatctgc aactaattga
     3001 tttaatactt ctttggtttg ttggaatttc atctttcttg cctcctaaca ttttcttaca
     3061 cttaaaataa tagtagactt tcaatgaaaa gtacaacaaa atcatttacc tgagaacgat
     3121 tttcagttcg cttttttatc cgtaaagcca atcatttttc agaatcagct aattcttctc
     3181 gcagttgagc aaattgctga taagtctgtg ctaaatgctt ttgtgtcgaa acaaataatg
     3241 gatagatttt ctgatatttt ttatgatcag tttcttgtac tccaacttgc tctttcaatt
     3301 ctatcaattc ctgtgcttca ctgatacttt ggatctttcc gatgcttctc cagcctaaaa
     3361 tcacagctcc taatgcggat gcttctgtga cagtagggaa aacaagcggg taacccagta
     3421 catcagccat catctgacga aaaatcggag attgagcaaa cccgcctgta gcacggatct
     3481 ctgtgagctg attttcctgg caaactcctt ttaaaatccg ataaaggttg agacagatcc
     3541 cttctagaat cgccttcatc atttcttttt ctgtatgttg ccgctgtaaa ccgataaaac
     3601 tgccaaacgc atctgcgtcc caaagcggtg ctcgctcacc aagtaaatag ggatgaaaaa
     3661 tcagaccatt ggcacccgct ggcacaaaag cgatagccga aagcagtgca tcatatggat
     3721 tttctccttt ttctttagca gattgaacga cttcctgtaa aaatgtatgc cctgcccaat
     3781 caaagatgcc agcaccattg ctagtcgctc ctcccactac ccaatgtttc tgatccagaa
     3841 cataacaaaa cgtctccatt tctggatgga actgaggctg agtagtaata aaacgaagtg
     3901 ctccgctcgt tcctacggtc aaagcggcga tcgtatcacc aaaagcacct aaccctagat
     3961 tagaaagcgg cccgtctgcc cctcccaaaa caaatgatat atcatttggc aaacctagtt
     4021 ctgctgctgt ttttgatggt agacctgtta cttgataggt ggaaggaaag acttgcggca
     4081 attgctctat tcgaatgttc aagtagtcaa gcacttcttc tgcccattgg aattcatgga
     4141 tatcaaaata tccagttcct gaagctgttc cgtagtcgct gatcagttcg ccaaacaatc
     4201 tataaaagat atagtctttg ataccgacaa aataagcagt ttttttgaat aattcaggtt
     4261 ggctgttttg cagccacttg attttgttca atggtgtcat tggatgaatc ggcgtcccag
     4321 tctgccgata aaattctttg ccaagcacgg tatatttcag tttttctgct tcgtccgctg
     4381 ctcggttatc tgcccaagtg atcattcgag ataacggtgt tttctcttca tccaacaata
     4441 tcaggccatg catcgcagag gagaaggaga caaattgaat gattgtgtct gaaggcaact
     4501 gtttaattac ttcttttata acggtgcaga caccttgaaa gatcaattcc tgatcttgtt
     4561 ctgccatccc ttgtgtttct tggatcaaag ggtagcgaaa ataagcggat gctgcttctt
     4621 gggcttcatc tgtgaaagca actgctttgg tttgtgtggt tccgatatct aatccgattg
     4681 aaacgttcat tttttcacct caaattttat gtatgtacaa aaatttagtt taagcttgcg
     4741 tatcttggtt ttgaaaaggg ggagatggcg tgttacttta ttttatcatt ggttgctgta
     4801 tcggatcatt tttatgttta accgcccagc ggattccgtt aggtcactct attatttatc
     4861 ctagatctca ttgtgtcaaa tgctgtcgtt cgctttcttg gtatgaattg attccgattc
     4921 tctcgatcat cgtccaacga tttcgctgcc gctattgcag gtgtcggttg cccttctact
     4981 atttgttggc agaagcctta tgtggtggtc tttttgcctg gttctttact ttttcttccg
     5041 caagaaattt ttcgacgttc atatggatgc tttcggcttt gctattttcg ttgatggatt
     5101 tattttactt catggtccat tctcacacgc tattctgttc ttgggcaatt ttatggattt
     5161 tttggttgca ggccggcgtc ttccaatggc aaagtgtgct gttaacgttt atcgtaggcg
     5221 gtgtgtgcct tcgctatggt caatcctatc tcggagctgg cgatactctg ttgatgcttt
     5281 cttggagtgg tggcttgtct ctagaagagt tgcttcagat cttgtttctt gctagtctgt
     5341 ttggaatatc cgttttttct gtttacttta tctgtcataa aaaaaagttg gaaaagctgc
     5401 ctttcattcc ttttttgagc agtgcgttgc tgattgtcct tcatcttagg tattgaaaaa
     5461 aggttgtgac aaacgacttg tcacaacctc tgatatatag tgttctctta ccaaatcctt
     5521 gtgacttgga gctaggcgac aggttcacaa cctctccact aaatggtgtt caatcagctg
     5581 accctcagta ttagctcaaa atgctggttt tacaacctct gatatacggt gttccataag
     5641 tagctttttc ggcttcttat ttcttgacgc cccaatgtcg acgaaggaaa cctgcacgtc
     5701 cagaaccttc tcgataaaga ttgtaatgga gcttgttttt cttgtaataa tcctgatggt
     5761 aatcttctgc aggataaaat tcgcttcttg gttcgatttt tgtcacgatt ggcttatcga
     5821 atcgcccgct gtcttgcaat gcttgttttg aacgctctgc aatatttcgt tcttcttcat
     5881 cattatagta gatcactgga cgataagaat caccgcgatc agcgaactgt cctaatgcgt
     5941 cagtcggatc ggtttgctgc caatagattt ccaccaactg atcataagaa ataacttctg
     6001 gatcatattc gatctctact gcttctgtat gccctgttgt gcctgttgtt acttgttcat
     6061 aagttggatt tggtacatgc ccgccagtat aaccggatgt cacagaaatg atccctggct
     6121 gctcttcaaa tggtttgacc atacaccaaa agcatccgcc tgcgaaaact gctttttttg
     6181 tcatataaaa tccccctttt ttctcatcat accttgattt ccaaaaggag gataacgttt
     6241 tgcttgtgac agaaaaaacg gaaactatta tatacgtgat acggcttctg ataaatgcca
     6301 aaacgaacgt acttgatcaa aaacggttaa atcaggaatc acatattcag cagaaggaag
     6361 atttatgttt tcacctattc ccaatacatg catgttacca gccaaagctg cttgacaacc
     6421 agcttctgaa tcttcaatga ccaagcatgc atttggcggt acattcaatt gctgtgctcc
     6481 tttgagaaaa acttctggat caggttttgc ttttgatact tgcgtgccgt caataaggac
     6541 atcaaaataa cttgtcaaat tcgttttttc taaaatgagg cgtgcatttt tgcttgctga
     6601 tcccaatccg atcttgatgt ttgttttttt caagtactca agaacagcta ttgcaccagg
     6661 caaaacggat gtttcatcca ttttttgaat cgcttgaaga tatagattgt ttttttgctc
     6721 agcaaaggct gatttctctt tttctgagaa tttctgttcc atttttccaa atgacagcaa
     6781 tcgctctagt gactcggttc tggatattcc tttcagttgc tcgttttctt tctcagtgaa
     6841 tggaatggac agctggttag ctaaaaccag ccacgcttcg taatgataac gagctgtgtc
     6901 tacaagaacg ccatctaaat cgaatagtgc tgctttgatt ttcatcgatt acctccttga
     6961 aaatgaacga cttgaagtaa agatgcgatg tttttagctg tgcgatgaag attatcagtg
     7021 gcttgctgta aagcttcttg taaaggcaca gctgcaggaa ggatcggaaa aacggccgaa
     7081 atgccaacat ctgctgcttt ttcgcttcct ttaccaacac ttccgcaaat agctatcaca
     7141 ggaacgccct ctggtactct tctagcaatc ccaactggcg cttttccttt tagactttgt
     7201 ccatctagac gtccttcgcc tacgattacg aggtctgctt gctgtacccg tttatcgaac
     7261 tccagtaaat ccaatacgta gtcgatgcca gagactattt tccctgaagc aaatgctaac
     7321 agtcctgcac ccattccacc accagcacta ctgcccgatg cctcgattac ttgaggggca
     7381 aatctcatat aaaaatcatg catggcttgg tcagccactg ctaattcatt ttctttcaag
     7441 cctttttgtg gtccgaaaac aaaagaagcg ccttcttttc cgcataaaag attagtaaca
     7501 tcagtaatga cagtgatttt ggctttaatc agctcaatag gcacgttcgt tttatcaact
     7561 gtagcaattt tacctagatt ttctcccagt gcttctactt tttgattatc ttgatcgtaa
     7621 aaagcataac ctagaccttc tgccatgccg atgccgccat cgttggtact acttccgcca
     7681 acgccgatga tgatgtgtcg aacaccttgt tgcaacaatt gtcgtatcag ttctcctact
     7741 cctcgcgtgg ataacctaag tggctgttgt tggtttagtg ggacatcttt caatccgacg
     7801 atttctgcca tttcaaaaat cgcagtttga ccgtctgaag cgtactggat ttctttgttt
     7861 ttgccaaatg cattagtaac gactgctgtt tgccgcttca accttaaact gtctgctaat
     7921 gatgaaaccg ttccttctcc tccatcgcct ataggtagca gatcatattc agcctgaaca
     7981 aaaacttctt gaaaacctga ttgaatggct tgtgctgcct ctaaggctgt cagactttct
     8041 ttgaaagaat ctggcgcaat cagaatacgc attattttcc cctccgttcc aagatcagtt
     8101 ctttttccag ctggtattgg tctccattga caaaaatagg taatgtttct ttcgtctctt
     8161 tttttattac tatttgtttt tttgtcaaaa caaatagcaa cctgtgattt ttccaaataa
     8221 aaggaaaaga aattttttcc catgctgaag gaagtcttgg agaaatagaa agagttccat
     8281 tcgttactgt aatacctgaa aaaccaaata taactgctag ccatagtgct cctaatgaag
     8341 cagcatgaat tccgtcatct gatgaatgcg gatctggtcc taaatcaatc aggcacgctt
     8401 cttggaataa atgatacgcc cattccattt cttggatacg gcaagcctct atggcgtgaa
     8461 tcgctttact caacgatgag tcgtggattg ttttgttttc atagtaatgc aaattcttag
     8521 aaacgattgg ttgagaaaaa tactcaggta ataagtataa cagcatgaca agatctgctt
     8581 gcttgctgac ttggagttca tttacttcgc tacgggcata atcaagtaaa atagactggc
     8641 tgccaggacg ttctttgtaa gtggtcaaat caataactgg cttactgaga tacgtgtcat
     8701 cttgcggaat cagctgttct ttgttaggca ctggcaaata taacttatct aaaaaatttt
     8761 gacattcttt caaaaaaagc ggatcgccag cttgatattt aacagctaag gccacattat
     8821 aatgcgccag atagttcgta tatgcattat tgtcgatata ttctgtgtat tcatctggac
     8881 caatgactct gtgtatctct aaatgatcat cgacttttac agctctgctc agccaaaaag
     8941 ctgctgtttc atgaatgagc gtttttccct ttgtttctaa aaacgattga tcctctgttg
     9001 cttggacata ctcttgaacg gcataagcaa ggtctgcact gatatgatgt tctgcggcag
     9061 ctgaagcaac ttcttgtctt gttccggttt tgatattcaa tgcagcaaat ttgggtgtct
     9121 cttcctgacc agtgtgtgcg ctttcccatg ggaaaagtgc accttgatag tgataatctc
     9181 ttgctttttg ccgtgcgcct tctaatcgat cataacggta cagcagcaaa ttctttgcta
     9241 cttcaggaaa ctgatataaa aagaaaggca ggataaaaat ttccgtatcc cagaaaacat
     9301 gccctttata accttcgcca gttagtccct tcgcccctac actaacactt ggatcattgc
     9361 ggggagtcat aatctgtaaa tggtaagcag cgaagtccat cgctaactga tcgaaagaag
     9421 aatcgccttg gatcgttatc cgatgctgat gccaaaaatc tgtccaataa gacatcgact
     9481 gctctaaaat ttctgtataa ctaacgcttt tctgggcttg ggcaatactt ttcttaaacg
     9541 ggtctgtttg ctgaagagag gtagagagca cgatgatctt ctccagcgta aaggacgtat
     9601 tttttctgat cttttctgaa aaggaagcgg tgatccggcg attttttgct tggaatacac
     9661 ctgctttatt gaatttagct gcaatcccaa tatcaatttt actttctgtt gtctggtagg
     9721 atgcactgat caaatcgtca ccaaatactc gcagttcttt ttcaaccaac tgctgtgtac
     9781 caaaattggt ttgctgtcca tcgatacccg ttttcacatc tacttggcag tcttcggtca
     9841 acggcttgat gaccactttc agggctgcct gatggagatt attttgatca gcaacacgac
     9901 aagtaatgac ttccactttt tttccttgag cagtttcaat gagcgcatag cgtttaagct
     9961 ctcccatttc taaatctagt gcccgctcgt atgcaagaac tttcatcgtc tcaagagaaa
    10021 aatgttcacc gtcgattttt atttcccatc tgatgacatc tgggagattc actaactcgg
    10081 ctgattctgc tcctaaaggt cggttatata caccagctgc aaacattccc cttacttgat
    10141 tccgatagtc ttcctcctgg cttcctctga taccaaaaaa tccatttcca atggtcatct
    10201 gactcgcata cttattgata tactctaaat caaactgttt ttcttctact ttcataaggt
    10261 aataacttct cctgtttctg ccgaacgata taaattttcc accagattct ggatgacata
    10321 tccttgccag ccatctgcaa tcatctgagt ctggtcaccg agcgcccgat tcacaaaatg
    10381 agccatactt agttcatgtg ggtcattaac tggcgtggtc ttttgtaagg aaaccagctt
    10441 tccatctaca tcttcgaata gttcgcctgg atacagatta gcacctgcct gatcgccaca
    10501 aaaagccaca ttcagtttgt cttctctgat attcaaagca aacgaagtat tcagttggat
    10561 gatcttgccg ttttctaatt ccagtgtgcc aaaaattgca tcttctactg tgaatttgct
    10621 tggatcccat ttcccgaatt tccctgaatt ttttttcgta ccaatttttt ggaagctgta
    10681 cgcccgaacg ctttttactt taggaaatcc agtaatatac atcgctgtat caagcatgtg
    10741 aatgccataa tcgatcaaag gcccgcctcc ttgaagttct ttgtcgataa acgttcccca
    10801 gcctggaaca ccagaccgac gaagcgcatt cgcttctacg taataaatat ccccgagaac
    10861 atcaagatga ttcttgatca ttgttgcttc gtcagaaaaa cgatgctgga atccataagc
    10921 caaaatctgc ccgtttttat cggctgtctc tgccatcttt cttgcttgat cagctgtcat
    10981 agctggtggt ttctcgcaca tcactgaagc attagcttcg agggcagcca atacatgttc
    11041 gaagtgaaag cgattgggtg tacagacact cacgatatcg ggagaaatct cattcagcat
    11101 ttgtgtaact gatgtatacg catgagaaaa tcctgtacgt cggcaaaagt cttctgctcg
    11161 ctggctgact ggatcaacca cagcggccaa ttctaagaca tctgatcttt tttgataata
    11221 agcagcatgt actttttctg ctacttgtcc agcgcctatg attgctactt tcttcattta
    11281 tttctctctc ctttgcttat ttacttattg ttattctttc tctattcttt ctctaaatca
    11341 tttaaatgac gcgctgtttc cagacagttt ttcaagtatt gcaatgattc ctgataaatt
    11401 ttcacttcat tttctcctcg caaacgacat tcatatacca tataaccttc atactcattt
    11461 tgaagtagcg tttgaaaatg gcgagtaaaa tcaattgcgc cagatcctgg ttggtagcga
    11521 tgattatctg ctaaatggat atgacccaca tactcttttt gcgtagtcaa tgcttttgaa
    11581 atatcatctt cttcaatatt catatgataa aaatcagcga tgatctgcac atgtttcaat
    11641 tgattagagt caatgtaatg acgcgcatcc gctaacaaat tgatcatatg gtcttggtat
    11701 cggttcaaag gctccaaata aataaccgta cctgtttctt cggctacttt atctaaataa
    11761 cgaagtgatt cgctgactgc tttatagtct ccttctttgc ttcggggtga aaccatcggc
    11821 ggtaatcgat aagtgaacat tccccaagct gctgggataa cgattccttt gcctccaacc
    11881 tcgcttagtg cacgcaaaat ctcagcaatt tgacgtacac cattaagacg ccgttcttcg
    11941 ataaagtcac cgatccaccc atcgtagccg ttacaagctg tcactactgg gatccctgtt
    12001 tctttgattg cctgtttgac tgtgtcgata ttttctatta acaacttacc gtcgatttcg
    12061 tattcttcaa atccacagtc tttgataaat ctaaattttt ctaaaatgtc tcttgggaaa
    12121 aatgcttgat cttgcgtacc gaatttcatt gttctcctcc tcggacttga acgcccattt
    12181 tgatactttc ttctgggtgc tggtctacat aacgcatgta agcttctgcg ctttctgcaa
    12241 atggcacaac tggatcaatg atttcttcac aatccaagta accgttcatc aataattccc
    12301 aacatgtgtc ctcgattcgt ttacggttcc atcttgggta atctgggttt ggttcactag
    12361 atgctcgagc aaagacgatt tttgcattat tgaaatgtgc ttcgcgacca agattaaacc
    12421 cagctggaaa aggtttagca aaggcaacat aagaaatgat cccgccatag ccgatcccta
    12481 ttaaagaagc ttgtaatgcg gcagcactgc cactcgtttc aataactgaa tctactccac
    12541 gtttatctgt caattccttt agacgaaaac cgacatctgc tttcatagga tctagcgtgt
    12601 aatcggcacc gtgtttttct gctaattctc tacgctgcgc tatgggatcg atcccaacga
    12661 tggtagtagc acctgctttt ttagctaatt gaatcgcaat ttgcccgata gcgcctaaac
    12721 cgaagattgc tacagcatca cctgctcgaa catgtgcttc ccggatacca cctaaagcga
    12781 attgagcagg atcgtaacat actgcatttt tccattgatc caaccgatca agtttacgta
    12841 gtttataatt atcaacaccg tttgtaatca gcgtttcacg aatgggtcca taagtgcaga
    12901 ctaactctcc tttttgatac tctgtcacca aactgcccgt ttcttcgatc gtaccgacaa
    12961 tcatatttcc taattgaaat tcgccaaata ccacaccgcg ttggcttcct tcttctcttg
    13021 gcaagaatag gcgccattct tctgaaaatt cttcatcgat aaatggactt aatcctctaa
    13081 agtcgacaat ttctgttcct ggttttggag tagcgaaaat tactcggata cgtacttcgt
    13141 tcttttcgat ctcacggtct tcgtaatctt tgaatccggc tattcttggt tctaatgcaa
    13201 ctaatttttt catcttttct ttcctctttc ttttcttttt atccttttac gccgccttcg
    13261 actaaatcac ctttaataaa tcgttcagat aacgcataca taatgatgac tggtaaagcg
    13321 gtcaccaatg aagcggccat cattcgtccc caaatgtagt ctggtgtact gaataatgtg
    13381 ttcaaaccga ttggtaaagt aaatttttcg gaggtcgaca ggaaaatcga agcaaataaa
    13441 taatcattcc acgaaaccat aaagcaataa acgaagacag aaatgattcc agaaatagcc
    13501 aatggtataa tgatccgaat aatgatctgg atccggctaa gaccatcgat cattgctgct
    13561 tcttcaatat cagtcggaat cgtgtcaaaa taacttttta gcatgaatat cgcagtagga
    13621 agtgtttgaa cgaccatcgt gataatcagt gctgtattgg tatcatacaa acctagaccg
    13681 gaaatgattt taaataatgg aacaacgatc aaaataccgg aaaacatata aacggtgtaa
    13741 aagctagcat tgattttcga gcgagctttg aagcgtagtt ttgacaaagc gtatgccccg
    13801 ctgatcccaa tcactactgc agttacggag gctatcaagg agatcactaa actatttttg
    13861 aaatacatga tgaatggaaa aatctcagca ttgaaaatat cctgataatg ttcaaaagtc
    13921 cattctttcg gaaaaatagt ttgtgtcgtt gcaattgctt cttgtgaatc cttgaatgat
    13981 gtcatcagca tgatcaaaaa tggaaacaaa ttgacagcta atagcaacaa aatcgtcgcg
    14041 taaaataaga ttttcctgct ctttttattt ttcatttcaa gtccaccttc tttcgtgaga
    14101 gcaagacgac cgctgcaata atgatgaaca atagaatcga tatagctgct gcttttccaa
    14161 gatcattgaa cgcaaaggct gtctgataca aataaacgcc taagatattg actttattcg
    14221 tcagtagata gacatctgta aacatataga acatccagat agatcgtaaa acaatcacag
    14281 ttgctagtac tggtttgatt gccggcagtg tcacgatctt gaacttctgc caagcatttg
    14341 caccgtccat ttcagctgct tcataaagcg ttttgtcgac tgtttgcaaa atagccaaaa
    14401 atgaaataaa cgcatacgga tagtatctcc agatagcaaa caaaacgacc ataaagaagc
    14461 tagcaactgg ctggtcgaac cacagcggtg cttcgttgaa aagctgcaac ctatctacta
    14521 acagatcatt gacgattcca taactattat tgaacatata tttccaagca aaaatcaaag
    14581 aaatagatgg cgtaacataa cttaataaaa tcagtgaacg caccgttttc ctaaatctga
    14641 acttgcggtt aagcaaaata gctacggcta atcctaaagc tgtactgcct gctacaacta
    14701 agaatgtgta agcaactgtt aaacctaaag aacgataaaa ttcactatca cttaaaattt
    14761 caagataatt gcttaatccg ataaaaacgg cgtctgagtt aggattcagc ggcatatcga
    14821 aaaaactgat ctgaatattg gaaagcattg gaatcagcac taaaacagct aataaaagca
    14881 tgcttggtgc taaaagtccc atggctaatt ttaagtcaga atgtttcctt ggacgattca
    14941 ttgaaacctc cccctgataa tctgccaata gctatctact tttttcgaac accattggca
    15001 gaacttcttg ttatttcgta tttttttgtg cgtcagctaa agcttgatcg acatcttcgt
    15061 ttccgacagt tacttggttc actgcttttc cgatcacacc tgagcttgtg atatctccca
    15121 ttttcgtgaa attcttttcc ccgactagtc caaagacttg tacttcatca aaagcactag
    15181 caatctcaga tggcagctcg ccaaaagact tgatgacttc attttctttg taaccatcca
    15241 gttcgactac ttgtttattc actggttgag caccaccagg agacatcaag acccactttt
    15301 ccatgttttt cggttgagat aagaaagctg tgaatgcttc agcagcctct ttttgtttct
    15361 cgtctagacc agaagaaatc gtcaatccag atactgtgcc atagacagct tgatcttttt
    15421 gtgttgggat agcaaaaccg atattttctg agttccccgc ttcataaaca gaaggcaaaa
    15481 tgtaagttga atagattgcc attggtgctg ttccattcat gaaagcatct ttgatttctg
    15541 tcacatcatt agagcctggc atcgtgtatt gggataagtt ttgataaaac gacaatgctt
    15601 ctctcatctt ttccgtatcg atcgttacct cgccttcatc atccaaaaca tttgctccat
    15661 ttgacaaagc aaattgagag aacgcttgct cactcattgt tccttcagca gtaggcatag
    15721 caattccata ttgtttattc tctttatctg tgaaatgttg agcaattttt aaaatatcat
    15781 cccaattttc tggttctgaa aaaccagctt ctgataattt ttctttgtca taccaaatac
    15841 cttgaaccca gccgctaatt ggtgcagcaa tatagctttt tccatcttct gaacgaacaa
    15901 ggtttttggc tccgtcataa taattatctt ctccagcttc ctcgataacg ttttggactg
    15961 ccgtctgatc aatcagttca tctttatcca tcactttagc aaaatcctga ctgacttcca
    16021 ttacttctgg taatttaccg gaacgagcta acgtaacgat ttttgtattg aacgctgatt
    16081 cttctaccgg cacttgcttg attttgatgt ctggattttc tttttcaaaa tcagcaacca
    16141 gttcattgat cacacttaat cgttcctgtt cgacagaaga gtgcatgaat tgaatcgttg
    16201 tctgttgtga ttcgttagag ctttgacttt caccacaagc agtaagaaga aatccagtag
    16261 ctagcagaca ggtagtaaca ataatttttt tcattttttt ccacctctta ttttattatt
    16321 ttcagccaca tatattcata cggctggaat atcaactttc gttcttgact gcaatggata
    16381 atttgtccag aaaacaggtc taggtaatct ccttttggta aaagataacc tgctgattga
    16441 ttggaaaaat tatggataac taagactttc tcttccttcg cttcgcgaat aaaactgata
    16501 aaagaatctg ctgagtccag taagtaaatc aaactattcg gatggaaggc tttttctttt
    16561 tttcgaatag atagcatctg ggtcaattca ccatatactg ctgttcttaa tggatctgtc
    16621 tgcaactctt tctcaatatc tgctaacaaa aatttttgcc gattgatttt tcgattctct
    16681 cctgtttttt ctacgccttt gtaatcattt cgacttccca gcacactttg aatataaatt
    16741 gccggcacgc ctggtaacgt taccaaaaat gaatgagcag ctaaaaagcg tttgcttctc
    16801 cattccgtcg tatctgttgg agaacttaac gcatctagat acgtagcgtt tatctcatat
    16861 ggactggtcg ttccatctgg gtttttcttg tagtttactc gggcaccctc tttcttcagc
    16921 tgttccacta attcaaggat cgttttttcc ggcagaatac ctcgtaccgg attcaatccg
    16981 ataccgtcat gggaggctaa aaaattaaaa aaagttgttt ttttcgatgc gaaatcgatg
    17041 gtttttaacc attctttcaa cttacttgta ttacccgaac gaatcgcttg aagggtcaaa
    17101 ggtggtagcg aaaactgata gaccatctgt gcttcattct cgccattccc aaaataactg
    17161 atattatctt tgtgtggcac atttgtctca gtgatcaata ccgttcctgg agctgcttca
    17221 tcagcgattg cacggaataa ttggatgatt ttatgcgttt ctggcaggtg tatacaagaa
    17281 gttcctagtt ctttccataa aaatcccaca gcgtccaagc gaataaatga agcgcctttt
    17341 tctaagtaaa acaaaagaat atcgatcatt ttcaataaaa cttctggatt tttaaaattc
    17401 aagtctactt gatcttcgct aaaagtcgtc catacgtgtt tttctttccc gtttttgtca
    17461 atgaacgttg taagtaacgg gctggtcctt ggtctagtca cgctgcttgt atctgtatcc
    17521 agtggcaaat caataaaaaa atctttgtac gtatcattca agtttaaata ttcctgaaac
    17581 caagtacttt cggcagacat atgattgcag acaaagtcga acattaaccg tgtttcgctt
    17641 ctcatttctt ctatatcttc ccatgtgccc aaacggtcgt tgattgcctg ataatttaca
    17701 acggagaaac catcatcaga tgtatatggg aaaaatggca agaaatgtac aactctaaat
    17761 gtatctgcca aatacttttg ataaaatttc ttaaatgtac gcaaagttgg ttgctgctct
    17821 tcaaaaaatt gatccccata agtaatcaag aaaatatctt cttcgtccca atgcagtttg
    17881 cctgcctggc ctttcttctg ctgataaaca ctgacttttt gttcaatcat agaggcggtt
    17941 ttctcagaca aatctgagtc gcgataaatc gcttgtaaaa gttttgtata catcggattc
    18001 ccctttcttt tttgggagcg ttcccaacga tttgatcata ttatggaaaa cgcttactgt
    18061 caatagcatt tttttaaata attttgatat acacaaaaat acaaataaga aagcttatgt
    18121 atcaataatt aatagcttct taaaaaaata tggtctctca tgatcatgca agctattcct
    18181 aaattttcaa aatcatagac aaaaaagaat gtctattgta ctatggaatt attctaaaac
    18241 gctcccaaaa aggaggtgca tataaaattg aaacctacta tttatgacat tgcgaaaaaa
    18301 gctaatgtct caaaatcaac tgtctccaga gtattaaata accagccaaa tatctctgat
    18361 aaagcaaaag caagtgtact tcaagcaatc aaagaattga attatgaacc gagcaaaatt
    18421 gcaagaggac tgtcaggaaa tggttttgac gcgattctag cattatctca tcgtacttct
    18481 catacgacct atggcaatcc ttttttttca gatgttattc aatccatttc tgccgtttct
    18541 gaacaaaata acttcgactt gattctacaa acggcaaaaa caccagaaga agagctagaa
    18601 aaatgcgcga aaaagatcca agaaaaaatg atccgaggaa ttattatttt aagctctacg
    18661 atggatgaaa cacttctttt tgaattggat caatataata ttcctattgt ggtaatcgga
    18721 aaagtcggaa acgactacac gaacatctac tcagtggaca cggataattt ccaggatagt
    18781 tacgatttgg tggaaacact gatcaagaat ggacaccgtc atatcggctg tctccatgct
    18841 ccgacagaag tttatgtatc caatgatcgt ctcaatggtt accgtcaatg cttatttgat
    18901 caccacctag atatccgtaa tgaatggatt atagacggcg gctttcgctt agaagatagc
    18961 atggcagcaa tgaatgattt aatgaagcta cgagaaagac caactgcgat tttttctacc
    19021 gatgcattaa aatcattgag tatttacaaa gtggcagaac aatctggaaa gcagatacct
    19081 gatgatcttt cgatcgcagg attcagtgat cccttattgt cctccctatt ttcgccacag
    19141 ttgactcgga tagacattcc gacaaatcag ttaggaatca aagccacaga acttttattc
    19201 caactaatcc accaccaata ccctgaagaa aaaagaatca ttctttcaac cgagattact
    19261 ctcacagatt cgatccaaca acaagaaatg tagaacagaa ttataaaaga acagaaacac
    19321 tgaggataag tcagttactc tgttcttttt tcaatcactc atccctcgtt accccctccc
    19381 ggtattttag cgaatttttc ttattttcca tctgttttag atagctgtgg ataactataa
    19441 tgtttgtgtt aaactatttt ttccacaggg aagaaaaaac tatttttaac cacgttgtgt
    19501 ataagttttt atagttcgtt tttttaaaca catttaccct tatttcaatc catgttattt
    19561 ggggatactt atctctaaaa cataaataga ataacagaca tgtctactgg ttttgtttta
    19621 ttcgaggaat tattcaataa tagctagcaa aatattaaaa ataaccgctc tatcttccca
    19681 tcaatttatt ttgcattcaa aaaatgatct atctttactt ttgttgattg acttctcttg
    19741 gaagatgatc gttccgatac cttaattgga tacgaaccga tgttgttaag atcctttccc
    19801 acttttcttc tagttagaca aattatttta gacaaaccaa aaaagaggtt gtgacaataa
    19861 tttgtcacaa cctctttttt aaaatatcta ttttgtttga ttgttcattg cgtcttctgc
    19921 agccatttgc gcttcaatat ctgaaatact gtagacattt tcgcttgcta caccgacttc
    19981 ttttggttcc atgtcacgat aacgttgcat accagtacca gctgggatga tcttacctat
    20041 aataacattt tctttcaaac caagaagagg atctttctta ccgcggatcg cagcatccgt
    20101 caagacacga gttgtttctt ggaatgatgc agcagataag aaactatttg tttctaatga
    20161 tgcttttgtg atacctagta agacaggtcg agaagttgca ggaacgcctc ctgagactag
    20221 cgtattgtag ttttgatctt taaagtctgc gatgtctaat aatgtacctg gtaagatatc
    20281 tgtatcacct ggatccatta cacgaacttt acgcagcatt tgacgaacca ttacttcgat
    20341 atgcttgtcg ccgatttcta ccccttgcat acggtaaaca cgttgtactt cacgaagcaa
    20401 gtagttttca acagataata catcgcgaac ttgcaacaat tgttttggat cgattgatcc
    20461 ttctgtcaat ggtgcaccac ggtggatcat atcgccttct gctactttca tacgtgctgt
    20521 ataaggaaca gtatacgtac gtgtatctgt ttttcctttg attgttactt ctttggtacg
    20581 tgttgcagga tcttctgaga tatcgatgac atcccctgtt acttctgtga tcactgcttg
    20641 ccctttcgga ttacgtgctt caaagatttc ttggacacga ggaagacctt gagtgatatc
    20701 gtctccggca accccacctg tatggaatgt acgcatcgtc aactgagtac caggttcacc
    20761 gattgattga gcggcgatcg taccaactgc ttctccgact tcaacgtcag agccagttgc
    20821 aaggttgcgt ccgtaacaat gtttgcagac accatgtttt gtattacatg tgaagacaga
    20881 acggattgtc actgtttcga cacctgcatc aacgatttgt tttgcgatgt cttcggaaat
    20941 caattggttt cctgcaatga tcaattcatt tgtttcagga tgacgtactt ctttacgggt
    21001 gtaacgtccc actaaacgat cttctaatga ttcgatgatt tcatttccct cgcggatggc
    21061 ttggatttca agacctcggt cagtaccgca atcttcttca cggatgatta catcttgggc
    21121 cacgtcaacc agacgacgag tcaagtaacc tgaatcggca gtcttcaagg ctgtatcggt
    21181 catcccttta cgagccccgt gagttgagat aaacatttcc aagacggata gtccttcacg
    21241 gaagttcgaa atgattggca attccatgat tcgaccatta ggagcggcca tcaaaccacg
    21301 cattccggca agctgtgtaa agttggaaat gttaccacga gctccagaat cagacatcat
    21361 gaagattgga tttttcgctt ccaaaccttc catcagtttt tgttggatgc tgtcttttgc
    21421 agcattccaa acagcaatga cacgttcata acgttcgtca tctgtgatca gaccacgacg
    21481 gaattgtttt gtaatagttt caacttgttt atgggcattt tcaatgattt cttgtttttc
    21541 attcagtaca ctgatgtcag cgatccctac tgtgataccg gcatgtgtcg aatgtttgta
    21601 ccctaagtct ttcatacggt caagcatctt agaagtttct gttactttga atcgtttgaa
    21661 gacttctgcg atgatgtttc ctaagttttt cttcttgaac ggtaatacta attcttgctc
    21721 tttgatatgt gcaggaatat cagtaccagc ttcaacaaag tatttgtccg gtgtttggac
    21781 ggtcaagttg aagtctgttg gttcattcag ataagggaat tcaggaggca tgatctcgtt
    21841 gaagatgatc ttaccaactg ttgtgatcat cgtacgttct ttttgccatt cagtaaatgg
    21901 tttttcacct aatgagttag ggttcacacc gatacgtgaa tgtaaatgaa catatccgtt
    21961 gcgccatgcg ataacggctt cgttttgatc tctgaagacc attccttcgc cttcgcgtcc
    22021 ttcttcttcc attgtcaagt agtagttacc tagaaccata tcttgagaag gagtaacaac
    22081 tggtttacca tctttagggt tcaagatgtt ttgagcagct aacatcaaca tacgtgcttc
    22141 tgcttgtgct tcttcattca atggaacgtg gacagccatt tggtctccat cgaaatcggc
    22201 attataagct tcacatacaa gtggatgcaa acggattgcg cgtccttgta ctaaaactgg
    22261 ttcgaatgct tggataccaa gtcggtgaag cgtaggtgcc cggttcagca atactgggtg
    22321 ttccttgatt acgtcttcta aaacatccca tacttcgtct tcttgacgtt cgattttacg
    22381 tttcgcattt ttgatgtttg aagctagttc gcgttgaaca agctcacgca tcacgaatgg
    22441 tttgaataat tcaatggcca tttcttttgg aagtccacat tggtacatct tcaagaaagg
    22501 tccaacaacg ataacggaac gacctgagta gtcgacacgt ttacctagca agttttgacg
    22561 gaaacgacct tgtttccctt tcaacatatg agaaagagat ttcaatgggc ggttacctgg
    22621 tccagtaact ggacgtccgc gacgaccgtt atcgatcaat gcatctactg cttcttgcag
    22681 catacgtttt tcattttgaa cgatgattcc aggtgcattc aagtctaaca aacgtttcaa
    22741 acggttatta cggttgatta cacgacggta aagatcattc aagtcacttg ttgcaaaacg
    22801 tccaccttct aattgaacca ttgggcgtaa atctggtgga ataacaggga taacgtccat
    22861 aaccatccag cttggttgat ttcctgaagc gcggaatgct tctaaaatat ccaaacggcg
    22921 gattgcacgt gtacgttttt gtccttgtgc agatttcaat tcttctttca attcggctgc
    22981 ttctgcatct agatcaacat tatcaagtaa ttgtttaatc gcttctgcac ccatagcagc
    23041 ttggaattct tgtccatatt gttcacgttt ttcgcgatat tcgcgttctg tcaacaattg
    23101 ttttttctct aaattcgtat ttcctggttc gatgactaca tatgaagcaa aataaatgat
    23161 ttcttccaaa gcgcgtgggc tcatgtctaa tacaagaccc atacgtgaag gaatcccttt
    23221 aaagtaccag atatgtgaaa caggagctgc taattcaata tgtcccatgc gttcacggcg
    23281 aactttcgaa cgagtaactt ccactccgca tcgatcacaa acgattcctt tataacggat
    23341 acgtttgtat tttccgcagg cacattccca gtcttttgta ggaccaaaaa tccgctcaca
    23401 gaacaaccct tcacgttcag gttttaaggt acgatagtta atcgtctcag gtttttttac
    23461 ttcaccgtaa gaccagcttc tgattttttc gggagaagcc aaacctattt gcatactttc
    23521 gaatttattt acatcgatca aaaggggttc cctccatttc atttaatggc aaaccgcaag
    23581 acggctgatc ttcgaccgcc ttgcagctca tagaccggat tagtctaaat tatcttctgc
    23641 ggtttcaaag ttttggatga catcgtcttt ttcatcttcc acttgttcag cagctttctt
    23701 ttctaattct ttggcagttt gctgttcggc aaattttgtc aaagcatcaa cagtgatcaa
    23761 atcgtcgtct tcatcgtcca tgtcgcgaag ttcgatctct gtttcttcga tatccaagac
    23821 acgcatgtcc agacctagtg attgcaattc tttcaccaat acgcggaatg attccggtac
    23881 acctggtttt ggaattggtt cacccttgac gattgcttcg tatgttttca cacgtccgac
    23941 tacgtcatct gatttgtatg tcaagatttc ttgtaaagta taagccgcac cgtaagcttc
    24001 cagtgcccat acttccattt caccaaaacg ttgtccacca aattgagctt tacctcccaa
    24061 tggttgttgt gtaaccaatg agtaaggtcc gattgaacgt gcatgcaatt tgtcatcgac
    24121 catgtgagca agtttgatca tgtacatcac accgacagac acacgtccat caaatggttc
    24181 acctgtacgt ccgtcgtaca gaacagtttt cgcatcgctg gccataccgg cttcacgaac
    24241 tgtttcccat acgtcttcat cacttgcacc atcaaatact ggcgttgcta cgtggatacc
    24301 taactgacgt gcagccatac ccaagtgtaa ttccaatact tgcccgatgt tcatacgtga
    24361 aggcacccct aaaggattca gcatgatatc gattggtgtt ccatcaggta ggaatggcat
    24421 atcttcttcc ggcataatac gggatacaac ccctttattt ccgtgacgac cggccatttt
    24481 atccccttca tggattttac gtttttgaac gatataaaca cgaactaaca tgtttacgcc
    24541 tggtgacaat tcatctccag cttcacgagt aaagattttc acatcgtgaa cgataccgcc
    24601 gccgccatgt ggtacacgta atgatgtatc gcggacttca cgagcttttt cgccaaagat
    24661 cgcatgcaat agacgttctt ctgcagataa ttctgttacc ccttttggcg tgactttccc
    24721 tacaagcaaa tcgccatctt ggacttctgc accaatacgg atgataccca tttcgtctaa
    24781 atcttttaat gcgtcttccc cgacattagg gatttcgcgt gtgatttctt caggtcctaa
    24841 ttttgtatcg cgggcttctg attcatattc ttcaatatgg atagaagtgt atacgtcatc
    24901 ttttaccaaa cgacggctca tgatgatcgc atcttcatag ttgtaccctt cccaagtcat
    24961 gaaaccaact aaaacgtttt ggcctagagc catttcgcct tgttccatag atggtccgtc
    25021 tgccagagta tccccttttt cgactttctc acccaaatga acgattggac gttggttgta
    25081 gcttgttccg gagtttgaac gacggaattt tgttacatcg tatttatcta atgcaccgtt
    25141 gtcgcggcgt acgcggattt cagaagcatc aacgtattca acgacaccgt catgtttaca
    25201 taacaaagca gctcctgagt catgcgctga tttgtattcc ataccagtac caacccatgg
    25261 tgcttgagga ttgatcaacg gcactgcttg acgctgcatg ttggcaccca tcaaggcacg
    25321 gttggagtca tcgttttcca agaaaggaat acatgctgtc gcaacggcaa ctacttgttt
    25381 aggtgatacg tccatgtagt ctactttatc aatagatact tctaagtttt cactttgagc
    25441 acgagccata acaacgtctt gagcaaatgt tccatcttca ttcaatgggc tgtttgcttg
    25501 ggctacgatg taatggtctt cgatatcagc tgtcaagtag tcgatttggt cagtaacacg
    25561 acctgtttca cggtctacac gacgataagg tgtttcgata aatccgaatt tatttacttt
    25621 cgcataactg gacaaactat tgatcaaccc gatgtttggg ccttcaggag tttcgatcgg
    25681 acacatacgg ccatagtggg agtagtgaac gtcacgaact tcataaccgg cgcggtcacg
    25741 agtcaatccg ccaggtccta aggctgaaag acgacgtttg tgtgtcaatt cgccaagagg
    25801 gtttgtttga tccatgaact gtgacaactg agaagaacca aagaattctt tgatacttgc
    25861 cacaactgga cggatattga tcaattgttg aggtgtcaat gtttcagtat cttggatcga
    25921 cattctttca cgaaccacac gttccatacg tgctagcccg atacggaatt ggttttgtaa
    25981 aagttcaccg actgaacgga tacgacgatt tcctaagtgg tcgatatcat cgacattgcc
    26041 gataccttcc atcaagttca agaaatagct cattgaagca atgatgtcgg ctggacgaac
    26101 cgtttttaca gctgcttctg gatagccgtt tccgatcacg tttacttcac gttcagggtc
    26161 tcttggtgaa aatactttga tgacttggac agtcattgga tctgtgacca caccgtcttc
    26221 tgatggatag taagtcactg aatttaggcc attatcaatg aaaggagcta gtgtttccat
    26281 gacttggtga gtcagaactg ttcctttttc aacgatgatc tcgcctgttt ctggatcaac
    26341 taatgtttca gctaatgtca gattcaatag acgtgttttc aaatctaatt ttttgtttac
    26401 tttgtaacga ccaacgtttg ctaagtcgta acgttttgga tcgaagaaac gagcattcag
    26461 caagttacgt gagctgtctg ctgtttttgg ttcgcctgga cgcaaacgtt cgtaaacgtc
    26521 tttcaaacct tcttctgttc ttgagtcgct tgcgttttta tgcaagtctt tttcgactgt
    26581 attgcgtaat gtttcgctgt cgccgaagat ttcaaagatc gtatcatcgg aaccaaatcc
    26641 taacgcacga actaatacgg ttaaaggaat cttacgtgta cggtcgatac gtacataaga
    26701 gatgtctttt gcatcagttt ccatttctag ccaagctcca cggttaggga taacagtaga
    26761 accgaatcct tcttttccgt ttttgtcgac ttttccatgg aaatagacac ctggtgaacg
    26821 gactaactga gaaacgatta ctcgctctgc cccgttgatg atgaatgttc cttgttctgt
    26881 cattaatggg aaatcaccga agaatacttc ttgtgctttg atttcgccag tctcgcggtt
    26941 tgtcaaacgc aatgttacat gtaatggtgc agagtagttt gcatcatgcg cacgagcttc
    27001 tgctactgta tactttggtt ctttcaattc ataatctaca aattccaaag atagattgcc
    27061 attgaagtca tcgattggca aaatgtcttc aaacatttct ctaagtccct catctaagaa
    27121 ccattggtaa gagtctgttt gaatttcgat caaatttggt aattccaaca cttcactgat
    27181 tcttgcgaaa cttctacgtt cgcgatgttt tccgtatttt actacgtgtc cagccaagct
    27241 cttcacccct ctaaaatgtc attcaaaaag actacccgat caattgttac aataatattt
    27301 caaatattat ttttaggtaa caacagacat tttttgcggc ttttaggcaa aaaaaaacca
    27361 aaaatagaac attctcttga aaccttactt cgtgagcttc tacttttgtt tccttatatt
    27421 aaagccgaaa cggacaatat ttcgtttctg ccttttcttc atcagatagt ttttgcaact
    27481 tcatatacta cacttatttt ctataaaagt caacacttcc tggcgtttct tctgcttttt
    27541 cgcaacattt tcttaatgtt ttcaatttta tgttttcaat tatctgacaa ttcttaattg
    27601 acagaccttt ttatctcggt tatactttag aaaattcatt ttgtggagga acttctatgc
    27661 gaaccaacat caaccaaatc gcaccaacac aactgaataa cttttacttt agctacttta
    27721 gataggtttg tattcatcat gatggataca aacgttaaac gacagtttgt atctatgatg
    27781 gctaaagaac tttgtattgc agcaaaaagc cacgtagaca aaatgctaag tggctgagca
    27841 gtgaaaatac aaagtaaaag tatcaagcat tctacttttt caatttattt tccgactttt
    27901 caatccattt ggacattttg tctgaatgga ttttttgttt cccaaaatta aggaggaaaa
    27961 agaaatgaaa aaaaatataa tcatcggatt aggcgctgcc ctgctactag ctttaggagg
    28021 ttgcgcacaa aaagacaatt ccgcagaggc atcagatgga caaaaaacaa ttggcgtatt
    28081 gcaacttgta caacataact cgttagatgc tgcctataaa ggattcaaag aaggattagc
    28141 agaagaaggg tataaagaag gcgataatct aacaatcgat tatcaaaatg cccaaaacaa
    28201 tcaagacaac ctgaaaagta tgagtgagaa actcgtcaaa gaagatcctg atttgctact
    28261 tggaatcgcc actccagcag cacaatcttt attaaatgaa acaaccacta tcccgatgac
    28321 tgtcacagcg gtcacagact tggaacaagc ccatttagtc acatccaatg aaaaacctgg
    28381 cggcaatgtg actggcacga cagatatggt gccaatcgaa aaacaaatcg gcttactgtt
    28441 aaatatcgtg ccagacgcca aaacaatcgg catcatgtat aacaacggtg aagcaaactc
    28501 taagatccaa gctgatttgg cagaaaaaga attgaaaaaa gcgggcgtca aagtcaaagt
    28561 actgacagca aattccacaa atgatgtcca acaagtgacg aaaagtctag ctaaagatgt
    28621 agatggaatc tatattccta ctgataacac gttcgcaagt gcggcaagtg ttgtcggtga
    28681 agtcgcaaaa gaaacaaaaa cacctgtcgt tgctggcgca gttgatcaag tcaaagacgg
    28741 cggtctagca acagtaggaa tcgactacga aagcttgggc aaacaaacag ggatcatggc
    28801 agcaaaaatc ttagacggca agaatccaag tgaacttcct gtcgaaacag cgaatgattt
    28861 ggaactgtat gtcaataaag acatggccaa agcattaggc atcaatccag acagcatcaa
    28921 agaagctgaa taaaacacag gaggattctc atgacaaatt tgctctctct tttcttatca
    28981 tctgcatcgc aaggggtctt atgggcccta cttgcgattg gtgtatacgt cactttccgt
    29041 atcttggaca ttgccgactt gactgctgaa ggaagctttc ctttaggtgc agggatcgcg
    29101 gcagtaagta ttacaaatgg ctatcatcca ttgacagctt gtcttctagg ctttttaggg
    29161 ggtgcggcag cagggcttgt ttccggactg cttcacacaa aattgaaaat tcccgccctt
    29221 cttgcaggga tcatcaccat gactggttta tattctgtca catcccgtgt gatgggtgcg
    29281 ccgaacatct ctttattggg tcagccaacg atctttcatt gggtcgagtc attcggcata
    29341 acaaaagaca acgctgtact gatcgtcggt ctgctttttg ctttgatcgt tgtatgttta
    29401 cttgttttgt tcttacgtac agaaacagga cttgctattc gtgcaactgg cgataatctg
    29461 gcaatgagcg aagcaaacgg gatcaacaca gataatatga agatcatcgg ttatatgatt
    29521 tctaatggat gcattgctct ttctggctcc ttacttgcgc aaaacaacgg ctttgctgat
    29581 ttaaattctg gtattggaac gatcgtaatt ggacttgctt ctattatcat cgcagaagtt
    29641 ttgttacgta accagccact atttctccgt ctcttgactg tcgttttagg tgcggtcatt
    29701 tatcgtttta ttttagcgtt agtcttcgaa ctgaatgttg aaccaagtga ttcgaaactt
    29761 gcttctgctt tcgtgctagt gatctgtctt tctttcccgc agatccagca aaaattccat
    29821 ttacctcaaa aaaagaaagg cggtagtgtc aaatgaatga cgtcttaaca ttggaaaatc
    29881 tccatcatac ctttgaaaaa ggaacgatca acgaaaatca tgtcctgaaa ggcattaatc
    29941 tttccttaga tcccggcgac ttcgtgacga tcatcggtgg aaatggggct ggtaaatcta
    30001 ctttgctgaa cagcatcgcc ggtacatttt ctgtcgatca gggtcaaatt ttattgaatg
    30061 gaaaaaatat cacgaaaaaa tctgttgtcg aacgttcgaa atccatcagt cgcgtattcc
    30121 aagatcctaa actagggaca gccgttcgtt taacggtaga agaaaatctt gctcttgcta
    30181 tgaaacgagg aaaaaaacgc ggcttcttcc gtggtgtcaa accacaagac cgaacatttt
    30241 tcaaagacca gctggttcgg ctaaatttgg ggttggaaaa tcgtttatcc tcagaaatcg
    30301 gtttgctgtc tggaggacaa cgtcaagcta ttacgttatt gatggccaca ttacagcgtc
    30361 cagaactgat cttattagat gaacacactg cagcgcttga ccctgctact tccaaaacgg
    30421 tgatggaatt gacagaaaag ctgatcactg agcagaatct cactgctttt atggtcactc
    30481 atgacatgga agacgcgatc cgctatggga gtcgactgat catgctgcat caaggaagga
    30541 tcgtcgtaga tatccagcaa gaggaaaaac agcagctcac agtgccccaa ttattagcgt
    30601 tgttcaaaca aaacagcggc acggcattaa aagacgatca agtactttta tcttcataat
    30661 cagaaagagg atgggacaaa aaatgaacgc ccaccctctt cttttttctc tttccttctt
    30721 tcgtttttgc tttatgtatc aaccgttgtt tcttcgaaaa ctattttgtt ctctccttct
    30781 tcctgataga atggatggga aaggagcttg atgccatgac aacgaaagac aaggtacttc
    30841 aattattgaa aaagacacca gattttcttt ccggagaaaa gttggcacag caactggaag
    30901 tttcccggac aagtatttgg aaagccatca aagaattgga aaaggcaggc tatcgttttg
    30961 agcacgggcc tacagggtat cgttacctgc cctctgacgt actagatgca aaagaaatcc
    31021 agcaagagct acggtcgttt attcctgatt tgaaagtaca aacgatgttg acttctgaat
    31081 ctacgatgaa agatgccaaa ctggcagcag ctcaaggaga ttctgtttcg aagttatttg
    31141 ttgctgatac acaagaacaa gctcgtggac gttttggacg gccttttttc gctgaacctg
    31201 gacgaggaat ctacatgagt ctcttgctcc atccagacaa acggtttgat gaattgcctc
    31261 aatatacgat catcgcagcc agtgcctgtg tgaaagccat tgaatctgta accggtcgtt
    31321 ctgcttcgat caaatgggtc aatgatatct acatggatgg aaaaaaagtc tgtgggattt
    31381 tatctgaagc agtcagtgat atggaaagcg gcaggatcag tcatatcatc attgggatcg
    31441 ggcttaattt ttctgttcct caaaatcatt ttccagctga tctccaagaa aaagccatct
    31501 ctgtctttcc cgacggacaa gctagtgtca caaggaatca gctaatcagc aaaatctggc
    31561 aagaattgtt cacttatctt tctcacccag aaaaagcgtt ggacttctat aaagaaaaat
    31621 cattcgtatt gggaaaaacc gtgtcgttca atcaaaaagg aacgacttat gtcggtcttg
    31681 caaaagaaat cggtgatcat ggcgaactga tcgtccaaac aaatgaacag aagatgagtt
    31741 tgtcttctgg cgaaatcagt ctcacatcta tccgtacgca ataattatca gatttcacaa
    31801 aaggacacct gatctcatac agtgtccttt tttctgaatt ctcttcaaaa gactctcctt
    31861 attctatcag ctgacagttt ctctatcctc tagaaaaagg tattctaaaa atatctataa
    31921 agcatggtac actttcttta aatgaatcaa tgcaaaaaac aagagagtca tcagtttaca
    31981 cgatcaaaag agatagcagg ccgcttcctt tttcaatctt atagatagac catgggtaac
    32041 gataaaaaca aaaaagaact tgcgacttat tttgccgtaa gttctttttt agctagtgcc
    32101 ttaatctctt aatcccaaca aacgtctcgc atagatcacc tgaccagagt gcatagcagc
    32161 atcatcaata atagaaacga gacgcattcc tcgtgtgact ggtggtgtcc agttctcgtc
    32221 aattacttca tctagctcgg aaggttccag ccttcttaag taagaaatag caaaatcagc
    32281 agctcctcgc aagtatccta gcagatcttc tatttgatta gcttggatct gctttgcttg
    32341 ttccagacta tgcttccaat cttgttcgtt atctgctatc gtcatcggaa accggctttc
    32401 ccagtcttga ctatgccaaa ttggagaagt tcctgctaaa tcagcaatct ggaaatctaa
    32461 ttctcgagcg gtatgccaag ccagccaagt cattgatttg atctgcgggg ctctatcttc
    32521 cactggaaaa cgattggcct cctcaataga gacgccttca aacaaacgtt caaaccgatc
    32581 gtatgcccga tccacactct cgcatagcat atctaaataa tccatttatt ttcctccttt
    32641 ttatttatcc tacttacttt ttcctaattc taggatacct acttatatgt tactacacac
    32701 ccttattttt tacactttaa cagtcaccat catcaatagt ttggagattg aaagaaaaag
    32761 atagtgtcga actatcaatt gaacaagaag caacaatatg aacgtttcat taatattctt
    32821 taaataaaca atgatttttc aaacgaacag tttaagacta ggagaaaaag agtataatag
    32881 gaaaatgttt gaaaggaaag gaaggggaaa aatgaaaaaa agggcaagtt tactagtcct
    32941 agttgctttg ttgttcaata tcttttcgta tggtgtggcc gtttcagcgg aaacatacat
    33001 tcctaaagac cctgggaata tttataatga tctaactggt aactatggct tgttaggaat
    33061 tgctagtcaa ttccacgtat ttgctaaaaa taagacaact gtgaatgcac atacagacgg
    33121 aaatatagca acacatgaac ttgatgcaaa aaataatttt ggtacagagc ttcatgaagg
    33181 tttattgaac caagaaataa attatataca aaaaactgat tctttcgcta attcttcagg
    33241 catacctaca ggtagcacaa gaatgaataa atttgtcgtt ggcagtaacg tggatgtagc
    33301 acttcaagat ggtcatccta cagtcaatgg atccaggctt gatcacttaa ctccagatga
    33361 tttttataaa gatcgtgctg ggcatgaata tattgatttc gagcaggaat ttgctaaatt
    33421 gaatgtagca gcaaatgatc ttgcaacgat cactccagct aaaacatata ctgctgcaga
    33481 ttttccagac atgaacaatc ggacaatcga tttaactgat ttatcagata ccgggttttt
    33541 attggtaaat atcgatgcag aagtgttaac gatgaatacg ccacttcaaa tcattaatcc
    33601 aaatgatcaa gtagttgtat ttaatgtcat caacagttct tctgcattga acgtacagtc
    33661 accaatcaaa tacaatgatc gttcaaatca tgaaacagaa gatttttcgg atgcgaatat
    33721 ctcttggaat tttggaaatg agatgactga tttgacaatc agtgctcctt ttcaaggaac
    33781 aatccttgcg cctaatgcta ctatccgcat tactcaaaac caagacggaa cgattatagg
    33841 aacaaatgtg attttagatg cagctaccaa tcgttgggat cctaacgaaa tatttatcag
    33901 cgacaatggt acagatacga cagacacaag cgacagtaca gatacatcta ccacaagtga
    33961 tagctcagat tcatcaactt caagcgacag tacagatact acct
//
LOCUS       DASJPC010000023        33925 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJPC010000023 DASJPC010000000
VERSION     DASJPC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.023"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            407..2068
                     /locus_tag="U4A13_001048"
     CDS             407..2068
                     /locus_tag="U4A13_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1779362.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2138..2485)
                     /locus_tag="U4A13_001049"
     CDS             complement(2138..2485)
                     /locus_tag="U4A13_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN1779363.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2569..3510
                     /locus_tag="U4A13_001050"
     CDS             2569..3510
                     /locus_tag="U4A13_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779364.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3511..4554
                     /locus_tag="U4A13_001051"
     CDS             3511..4554
                     /locus_tag="U4A13_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779365.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4560..5630
                     /locus_tag="U4A13_001052"
     CDS             4560..5630
                     /locus_tag="U4A13_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779366.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5620..6573
                     /locus_tag="U4A13_001053"
     CDS             5620..6573
                     /locus_tag="U4A13_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1779367.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6585..7595
                     /locus_tag="U4A13_001054"
     CDS             6585..7595
                     /locus_tag="U4A13_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1779368.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7880..8701)
                     /locus_tag="U4A13_001055"
     CDS             complement(7880..8701)
                     /locus_tag="U4A13_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1779369.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8798..9076)
                     /locus_tag="U4A13_001056"
     CDS             complement(8798..9076)
                     /locus_tag="U4A13_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779370.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9344..11353
                     /gene="metG"
                     /locus_tag="U4A13_001057"
     CDS             9344..11353
                     /gene="metG"
                     /locus_tag="U4A13_001057"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN1779371.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11832..12419)
                     /locus_tag="U4A13_001058"
     CDS             complement(11832..12419)
                     /locus_tag="U4A13_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779372.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12594..13916
                     /locus_tag="U4A13_001059"
     CDS             12594..13916
                     /locus_tag="U4A13_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1779373.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14071..14847
                     /locus_tag="U4A13_001060"
     CDS             14071..14847
                     /locus_tag="U4A13_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1779374.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14840..15409
                     /gene="rnmV"
                     /locus_tag="U4A13_001061"
     CDS             14840..15409
                     /gene="rnmV"
                     /locus_tag="U4A13_001061"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN1779375.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15402..16286
                     /gene="rsmA"
                     /locus_tag="U4A13_001062"
     CDS             15402..16286
                     /gene="rsmA"
                     /locus_tag="U4A13_001062"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN1779376.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16737..17366
                     /locus_tag="U4A13_001063"
     CDS             16737..17366
                     /locus_tag="U4A13_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779377.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17490..18599)
                     /gene="ugpC"
                     /locus_tag="U4A13_001064"
     CDS             complement(17490..18599)
                     /gene="ugpC"
                     /locus_tag="U4A13_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN1779378.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18808..19230
                     /gene="mgsA"
                     /locus_tag="U4A13_001065"
     CDS             18808..19230
                     /gene="mgsA"
                     /locus_tag="U4A13_001065"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN1779379.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19553..20089
                     /locus_tag="U4A13_001066"
     CDS             19553..20089
                     /locus_tag="U4A13_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1779380.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20129..21892)
                     /locus_tag="U4A13_001067"
     CDS             complement(20129..21892)
                     /locus_tag="U4A13_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779381.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21895..23610)
                     /locus_tag="U4A13_001068"
     CDS             complement(21895..23610)
                     /locus_tag="U4A13_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779382.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24389..24613)
                     /locus_tag="U4A13_001069"
     CDS             complement(24389..24613)
                     /locus_tag="U4A13_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779383.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24804..27581
                     /locus_tag="U4A13_001070"
     CDS             24804..27581
                     /locus_tag="U4A13_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1779384.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27662..28081
                     /locus_tag="U4A13_001071"
     CDS             27662..28081
                     /locus_tag="U4A13_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN1779385.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            28093..28563
                     /locus_tag="U4A13_001072"
     CDS             28093..28563
                     /locus_tag="U4A13_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1779386.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28580..29359
                     /locus_tag="U4A13_001073"
     CDS             28580..29359
                     /locus_tag="U4A13_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1779387.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29346..30167
                     /locus_tag="U4A13_001074"
     CDS             29346..30167
                     /locus_tag="U4A13_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1779388.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30184..31233
                     /locus_tag="U4A13_001075"
     CDS             30184..31233
                     /locus_tag="U4A13_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1779389.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31246..32250
                     /locus_tag="U4A13_001076"
     CDS             31246..32250
                     /locus_tag="U4A13_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1779390.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32883..33902
                     /locus_tag="U4A13_001077"
     CDS             32883..33902
                     /locus_tag="U4A13_001077"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN1779391.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11075 a         6006 c         6929 g         9915 t
ORIGIN      
        1 aaagtcgctt agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg
       61 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttattc agataaggga
      121 ctgtgacaag acttttgtca cagtttcttt ttgtttattc ttttttatct ttgtatggca
      181 ataatagaca atataacgaa aattatttaa tggcttttgg aagaccatgg aaaaccttgt
      241 acaaagctat tttttctgaa aaattccttg aaacctcacg aaaaaatcat tattttatga
      301 aaaagacaaa aaaaagtgtt gattttttaa taattattca ttaaactctt aaacaacttc
      361 agaaaatatt aattattctg aaaattccaa taaaggggta cgtaaaatga aaaagaaatt
      421 tacatttggt ttggttgcgg tatgcggcct tgtacttgct ggatgttatg gcggcagttc
      481 agccacaagc agtagttcag caaaaagcgg ttcagcagat ggaggaggcg tatttaactt
      541 agtcgtgcct caagaaatgc ctacagctga cttatcagtt gcaacagata caatcagttt
      601 tacagcattg aataacgttt atgaaggaat ttaccgtctt gatgaagaca gcaaaccaca
      661 gccggcagga gctagcgagt tggcagaagt cagtgaagac ggattaactt acaaaatcaa
      721 attacgagaa gacgcaaaat ggtcaaatgg tgatcctgtt acagcagcgg attacgttta
      781 tggctggcaa cgaacagtag acccagctac agcttctgaa tatgcttacc tgtatgcgcc
      841 agttgaaaat gcagaagaca ttacagctgg aaagaaagat aaatcagaac tagggatcaa
      901 agcagttggt gactatgaat tagaaatcaa attgacgaaa caaacaccat atttccaata
      961 tctgcttgct ttcccatcat ttttcccaca aaaacaatcc gtagtggaag aaaatgggga
     1021 tgcctatgca tctgcaagtg ataaagctgt ttataatgga ccattcacat tagaagattt
     1081 tgatggacca ggaacagata cagaatggac ttataagaaa aatgatcatt actgggacaa
     1141 agatacagta aaactttctg aaatcaaagt cagtgtagta aaagaatctt ctactgcatt
     1201 gaacttattc aaagatggcc aagcagatga cgtgatcttg tctggtgagt tagcacagca
     1261 aaacgcaaac gatccagctt atacttctgt taaagaagcg cggacaagct atatcgaatt
     1321 caatcaacgt gaaaagaatt caccattcaa caatgtgaac ctaagaaaag ctatttctta
     1381 ttctatcgat cgtgaagctt tagtaaaaca agtactaggt gacggatcag ttgtctctac
     1441 aggattgatc ccatcagata tggtaaaaag tccaacaacg aacgaagact ttgcaaaaga
     1501 agctggaaaa ttagtttctt atgacaaaga taaagcaaaa gaatattggg aaaaagcgaa
     1561 aaaagaattg ggcgttgatt ctttagaatt tgatttgatg gcttctgatg atgattcatc
     1621 gaaaaaagta atcgaatatg ttcaaaactc aatccaagaa aacttggatg gcgtaacagt
     1681 caaaccaaca cctgttccat tctctgtacg tcttgaccgc tcaacttcgg gtgattttga
     1741 cgcagtacta ggcggatggg ctgctgacta tgcagaccca agcagcttta ctgatttgtt
     1801 tgtcacaggt aattcataca accgtggtcg ctggagcaat gcagaatatg acgaagcagt
     1861 taaagcagct tcatcaacag acgctggcga tgaacaagcc cgttgggatg atttccaaaa
     1921 agcaatcaag atcatctctg atgatatggg tgtcgttccg gtgtaccaaa aagcagaagg
     1981 tcacctagtg aatgaaaaaa tcaaaggtat cgttcaccat gcggcaggcg cttcatggga
     2041 ttacaaatgg acatacgttg aagaataatc gttaataccg tgagcatgag aaaaaacgag
     2101 gctgcggcaa atgtcgcaac ctcgttctca tattcactta tcctaacaaa gaaaaaagca
     2161 tgaaaatagc agaaagagca attagtattc cggcaatgat cagccaaaag ctgtacatac
     2221 cgtagctggc agacgagagc gaggaaaatt ctggtttttt ctttcggttc cttgttcgtg
     2281 caagatggac cgatcgctga aaatgatcga agaaacccga agaaaagacc cataaaaaac
     2341 cgccgataat cagaaacggc aatgaccata agaatagatc attagataat tgaatgagtg
     2401 taaaggaatg gttgatgaga ttttttatta aaataaccag aatcagtaga aaagctactg
     2461 cgtaggggat agattttttt ttcatttgat tgctccttta tttttggttc attatctaat
     2521 aatgtagcga aacatctgtc agaagtcaaa agctaggggg aaacattagt gaatagttat
     2581 atcaaatatg tattaaaacg tgtctttttc atgatcatca cgctttggtt aattgcaaca
     2641 atcacctttt tcttgatgca attgctgccg ggcacgccat atacgaatca ggaacgtttg
     2701 agtcctgaaa caatcgagat gctaaataaa caagtcggtt tggataaacc agttatcgta
     2761 caatatggga tttatctttc taatttgctt caaggagatt ttggaatttc cttccaattt
     2821 aaaaaccagc cagtcgcaca tttgttagct ggaagaatcg gaccatcctt gcagttaggg
     2881 ttacaggcaa ttatctttgg aacagtccta gggacgattt taggaacgat ctctgccatg
     2941 aagcaaaata catgggcaga tacatcttct acactcgtag cgatattagg tcgttccatt
     3001 ccaaactttg tatttgctgt tttattacaa tatatctttg caatcaaatt acaagttttg
     3061 ccaatcgcaa aatgggatgg ctttgtgtac acaatcttac caacaattgc gttggccatg
     3121 tcgccgcttg ctgactctgc tcggtttatc cgtacggaaa tggtggaagt actgcatagt
     3181 gattatgtgg aattagcaaa agcaaaagga ttgagccgtt gggaaatcgc ctttaaacat
     3241 ggtcttcgaa atagtttgat tccattgatg actttattag gaccgttagc tgtagcattg
     3301 atgaccggat cactggtggt agaaaatatc tttgctattc ctggtatcgg ggaacaattc
     3361 gttaagtcaa tcacaacaaa tgattatcct acgatcatgg ctgtaacgat cctttactca
     3421 tttatgctga tttttgttat tttgatcgtg gatctattgt acggtctagt tgatccaaga
     3481 attcgtgttt cagaagggag ccgaggataa atggaaatga taccaaagcg ttatgaaaca
     3541 gttgcagata tccctgcgga tgaatttctt ccattacaaa aaaataccga agaagaacgt
     3601 gaacagatta ctgcaccttc tttgaatttt atccaagatt catggcgccg tttaaagaaa
     3661 aacaaagctg ccgttatttc aatggtttta ctattggtca tcattttgat ctctgtgatc
     3721 acaatctttg tttctcctca tgatccaact gcccaaaacg tggcttatat caatttgcct
     3781 cctcgtattc cgggaatcaa tatcgatgga ttgaatggaa aagcaatggt tggtggtgaa
     3841 cttgttgata aatatgctca agcaaacgtc ccttcaaacg taaatttctt tttaggaaca
     3901 gacggtttgg gacgtgatgt attaagccgc ttatttatgg gaatgagaat ctcgttgctg
     3961 atcgcattta ttgctgcatt actagatgta acgattggtg tagcttacgg gttggtttct
     4021 ggtttgttag gcggacgtgt cgataacgtg atgcaacgtt ttttggaaat cttatcagga
     4081 attccaaacc tcgttgtcat gattttgatg ttggtcgttt ttgaaccagg aattttttca
     4141 atcgtggctg ctatggccat cacaaactgg ataccaatgg cacgtatcgt tcgtgcacaa
     4201 acaatgaaat taaaagatca agaatatgtt ttagctggat tgacattagg tgaatcaaga
     4261 atgaaaattg cattcaaaca tattttgccg aatatctcta gtgtcattat tattcaaatg
     4321 atgtttagta ttccatctgc tattttcttc gaagcctttt taagctttat cggtttaggt
     4381 cttacgcctc cgtcagcttc attaggaacg atgttgagcg aagggtacaa gacattcctg
     4441 tatctgcctt atctattatg gattccggca gtaactttat cggttatcat gattggcttt
     4501 aacttacttg ctgatggatt gcgtgatgcc tttgatccta aaatgaaaga gtgaagacga
     4561 tgacgaaaat tttagaagtg aaagacttag aaatctcttt tgatacgtat gctggaaagg
     4621 tacgagcgat tcgcggtgtc aattttcatt tgaataaagg agaaacattg gcgatcgttg
     4681 gggaatctgg aagtggaaaa tcagtgacga ccagaagcat catgcgcttg ctgtcaagca
     4741 acgccaatat agatgctggt caaatcctct ttaaaggtaa cgatatcgtt ggaaaatcag
     4801 aaagagagat gcaaaaaatc cgtggtaaag aaatagcgat gattttccaa gatccaatga
     4861 cctctttaga tccgacgatg cccattggga agcaagtcgc tgagtccctt agaaagcata
     4921 ataaagtttc aaaaaaagag ggacaaaaag ctgcattaga attgcttaaa ttagttggaa
     4981 tacccgaagc tgagaaacgt atcaacagtt atccgcatca gttctcagga ggtcagcgtc
     5041 aacggatcgt gatcgcgatt gcactggtat gttatccgga aatcctgatt gctgatgaac
     5101 ctacaacagc gcttgatgtg actattcaag cacagatttt ggagctgctg aaagaaattc
     5161 agacaaaaat cgatacatca attatcttta tcactcatga ccttggtgta gtagcgaatg
     5221 tcgctgatcg tgtcgctgtc atgtatggag gaagaatcgt tgaagttgga acatcggaag
     5281 aaattttcta caacccgcaa catccctata cttggggact gcttggttca atgccgacat
     5341 tagatagtgc gaacgatcga ttgtatgcta ttcctggttc tccaccggat ttattgaacc
     5401 cgccaaaagg agacgcattc tatccaagaa atgaattcgc gatgaaaatc gatgcagaac
     5461 aagaaccacc attttttgaa ctatcaaaaa cacacaaggc agcaacttgg ctacttgctc
     5521 cgcaggcacc caaagtgaca ccgccagaag aaatccaacg tcgctgggct atctttaaag
     5581 agaaacaaaa acatacttac ggtcaaggag gaattgctga tgccaaataa actacttgaa
     5641 gttaaaggat tgaaacagta tttcaatgta ggaaaaaaga atgaagtaca tgctgtaaat
     5701 gacatcagct ttcacattta tgaaggagaa acattcggtt tagttggaga atctggaagc
     5761 ggaaaatcca ctactggccg tacgatcatt cgcttgaatg aacctactga tggtgagata
     5821 ttattcgacg gtcaagatat aacgaagatc aaagataaaa aaggtttgac gaaattccgg
     5881 catgatgttc agatgatctt tcaagatcca tacgcttcgt tgaatcctcg tatgaaagtc
     5941 agagatatca ttgccgaagg aattgatgtt aatggactag ctaaatcatc tgaagagcgt
     6001 gcaaaaaaag tcgatgatct attgagaact gttggattga atccaagcca cggtactcgt
     6061 tatcctcatg aattttcagg tgggcaacgc cagcgtattg gtattgcacg agcattggca
     6121 gtaaaaccgc gttttatcat ttgtgatgaa ccaatttctg cactagatgt gtcgatccaa
     6181 gcccaagtgg tcaatttgtt gcaagatctc caaaaagaac atcaattgac ctatctattt
     6241 attgcacatg atttgtcgat ggttaaacat attagtgacc gaatcggtgt aatgcataac
     6301 ggcttgttat tagagatggg aacaagtgat gaaatctata atcacggtgt tcatccttat
     6361 acagaaagtt tgttatctgc tattcctcta ccagatccag accatgagag aaaacgcaga
     6421 agaatcaaat atcaacctga accagatgac ggacaagtac gtcaacttcg tgaaatcgca
     6481 ccagaacatt ttgtctatgc aactgagcag gaagtcccat attacgcaaa aaaactaaaa
     6541 cgacaaaagg aatctcttct ggttgctaac taggagggac atagatgcta aaagctttta
     6601 aattccgtat gtatccgaca gaagaacaaa aacagcaatt gattcgtact tttggatgtg
     6661 ctcgatttac ctataaccat ttgctgaaaa agcggcagaa aagttggcaa caaacaggag
     6721 tagccaattt ttcgctcaca cccgctacat taaaaaaaga atacccattc ttaaaagaag
     6781 tagacagtct tgctttagca aatgcacagc ttaatttgga tcgggctttt cgcaattatt
     6841 tcaagggacg tgccagcttc ccaaagctta aaacgaaaaa aagtatgtgg caatcttata
     6901 cgaccaacaa ccaaacccgt acgatctatc tggaaaatgg ctacttaaag ttaccaaaac
     6961 aaaaagaact aatcaagata aatcagcatc ggccagttga aggatcgatt cgctccgcta
     7021 cgatttctgc tcgatataat gaagaatttt atgtcgcact actttgtgat gtttcgccag
     7081 taaaaaaaga atctttagct aaatggatcg ggatcgccta tcatccgaag acattgatcc
     7141 aaacatctcg acctctagaa gtaactttac caaaatttca ccagacagaa gaaaagctgc
     7201 aacatgcaca gcggaaattg aatgtgaaag tccgctccgc tcaccataga aagatcaggt
     7261 tagatcaggc aagcaattat cagaaacaaa agagaaaagt aatggatctt tatctgaaac
     7321 aaaagaacca gcgagaagat tatcttgaac aattatctgg gaaattggtc aaacaatatg
     7381 actacttatt tgtagaatct tttccaaaag aagaggcaca tgctgatttt tctattcacg
     7441 actggcataa attaataaca aaattgcgtt ataagtccca atggtataat aaaaaatttc
     7501 ttttgatcaa taccgacgga gcagaagaaa gcaattccgt aagaaagagc caagtagtag
     7561 aaaaaatggg cagacattcg gttatcaagg gataaaaaat gaatgaaact gataggagcg
     7621 ttagggttag ccttgttaat aaaaagtctt aatgactttt gtttctagga atttttcttt
     7681 tgaagaaaag ctcactaatt atgacaaacc aataaaacaa tagcagaaaa agttcgtacg
     7741 agctttttct gctattgtta attttttatt cagaaagttt acgaatacat caaaattctg
     7801 aaacaaaaaa cgtaactcac aaagagttgt ttttgcgttt tttcttgaat gattaggtct
     7861 gaatcttttg ctcacaacct tattcatggt gccccggctt gagctgatag atttttgaag
     7921 gacggccgat tcctacagga cgttcaccga tctcatcgaa atattggagc atagctttct
     7981 taaaattgga atgatcgatc gttttataat cgatccctaa gaatttagca aatacttttc
     8041 gagcttccgt aatcgtgaaa tctttaccta aaacttgtag aacttgcggc tcgtgttcca
     8101 ttttattgac tacacggttg aatgctttca agatgatttg actatggtca aaagccaaag
     8161 tatctttgcc agaagactct ccagtgacta aatcaagcga gatagccact tctccgctag
     8221 ataaatggat cttgttacta tgacgttcta gtgtgaacca gcgtacttcc ttggcatcat
     8281 ctccggcgat tagcggttct tctccaataa aggctaagta gctgactgtg acgacccagc
     8341 ctcgcggatc tcgattaggc gtactgaatg tatgcagttg ctcaatattt tcttttgaaa
     8401 taaccacacc agtttcttcc tttgtttctc ttaagacgct ttctccagtt gactcatcct
     8461 tttggacaaa gccaccaggc aatgcccatg aatttcgata aggatgccct tttctttgga
     8521 tcaacaaaac tttcagctga tcttcttctt tgttatagca catcagtacg atatcgacag
     8581 tcaaagaggg cttgtcatat tctggacgtt cctgtttttg ataccagtta agaaattctt
     8641 cttcacttgc tttatgttca taatataatt tttcttcttc tttagaagca aaacggttca
     8701 tctgaccacc tccattgaca gtaaaagtac tttttacctt attgtagcag actctgaaaa
     8761 aaaaccaagg atttacttga aaaatctagg ttatcattta aatatgaaaa ataaatggaa
     8821 gtatgagatt gaataaaaag aaaagcagat aaaacggcaa tactttatac cgctcttcac
     8881 gaatggcaaa gaagccacaa ataacgatat atacgtcaat gataaaacgc aaaatccaag
     8941 ctgctgtcat aaaactgttc aaatgcccat ggaggttaaa ataaccatac atacgaatat
     9001 aaaaatagct gaatattaaa tgtgctaatg aaagaaaagc aatactaagt gtaaaaaaaa
     9061 gatgtttctt ctgcatgatt gttccagtcc cttactaaga tagatagtct ttattatacc
     9121 acgtgtgagg ggagaaataa aaagccgttt tttagtagat atgaaacaaa acaaaaaccc
     9181 gcatttattc aacctattca tccttttaaa ggatagattg aacgtagata gaagtcgttt
     9241 atccagtctt tttcattttg aaaaaaagct aacagcatgc tttttttatg gtatactagc
     9301 aaggaaacga attcgcatca tgcgataaag gaggaagagc cccgtggcag aaaaggaaac
     9361 tttttatatc actacgccaa tctattatcc aagcggcaaa cttcatatcg gcaactcgta
     9421 tactacgatt gcatgtgacg caattgctcg ttataaacgg ttgatgggct ttgatgtgtt
     9481 ttatctcact ggagtggatg aacatggtca aaaaatcgaa acaaaagcag aagaactagg
     9541 tgtaaaaccg caagaatatg tagataaaat ggcggctgat gtcaagaaat tatggaaaac
     9601 gcttgatatc agttatgata aattcattcg cacgacagat gattaccata aagcagctgt
     9661 tcaaaaaata tttgatcgtt tattagaaca gggagatatt tacttaggtg aatatgaagg
     9721 ctggtactct gtttctgacg aagaattctt tacagaaaca caacttgcag aagtgtacaa
     9781 agatgacgaa ggtaacgtga tcggtggtaa agcgccaagt ggacacgaag tagaattggt
     9841 aaaagaagaa tcatacttct tccgcatgag caaatacgca gaccgtctat tggaatacta
     9901 tgaagaacat cccgaattca tccagccaga atctcgtaaa aacgaaatga tcaacaactt
     9961 tatcaaacca ggattagagg atcttgcagt ttcacgaaca acttttactt ggggagtacc
    10021 agtcaagagt gatccgaaac atgtggtgta tgtatggatc gatgcattat ccaattatat
    10081 tactgcacta ggatatggct ccgaagatga aagtctattc gaaaaatact ggcctgcaga
    10141 tgttcagatg gtcggtaaag aaatcgttcg ctttcatacg atctattggc caatcatgct
    10201 gatggccctt gatttgcctc tacctaaaaa agtatttggg catggctggc tattgatgaa
    10261 agacggaaaa atgtctaaat caaaaggaaa tgtcgtttat cctgaaatgc ttgtggaacg
    10321 ttatggttta gatgcactcc gttattattt gcttcgtgct gttccgtttg ggtcagatgg
    10381 tgtctttacg ccagaagatt ttgtttcccg tgtcaattat gatctggcaa atgatctagg
    10441 gaacttattg aatcgaacga ttgctatgat caacaaatat tgtgatggac tcgttcctga
    10501 ctatgcctca cttgtcactc catttgatag tgaactgtct acgacggctg ctaacgttgt
    10561 cggacgttac catgatgcaa tggaaaaaat ggaattcaat acagcattgg ctgaaatctg
    10621 ggtgctgatt tctcgtgcaa acaaatacat tgatgaaaca gaaccatggg tattagcaaa
    10681 agacgaagaa aagaaagctg aattgaatag tgtcatgatc catttagctg aatctttgcg
    10741 tatcgcagcg atattacttc agccaatcat gacagaaacg ccagagaaga tcttcaatca
    10801 actaggattg gactctgaaa caatgaatct tgaaggatta cactttggcg aatttccttc
    10861 aggaacaaaa gtagttgcaa aaggtacacc aatcttccca cgtttagata tggaagaaga
    10921 agttgctttt attcaagaaa aaatgtctga aggtactcaa acaaacgaag atacagtcaa
    10981 atgggatcca gaagaaacag aacttgtttc aacgaaagaa aaacaaatca aatttgatgt
    11041 atttgaaaaa gtagaactaa aagtagcaga agtcatcaac tgtcaaaaag tagaaggggc
    11101 agataagttg ctgcaattcc gactagatgc tggagacagc caagaccgtc aaatcttgtc
    11161 aggaatcgct gaattttatc ctgatccaag cgaattgatc gggaaaaaag tcgtcattgt
    11221 agcaaatctg aaaccacgaa agatgcgtgg acaaatcagt caaggaatga tcctttctgc
    11281 agaagcacca gatggctctt tacaagtaat cgaagcacca aaatccatgc caaatggctc
    11341 agaaatcgca tagaaattca aagggggtat gacactagtt gtgtcatacc ccctttttcc
    11401 gaaatttgag aagcaatttt cgtaaattcc ttcttattac ttgaagccaa tcatataata
    11461 cagaaacaga gaactatcca aaataaatcc tagaaaaaga agtacgagct tcgctgattt
    11521 ttgttaattt agaataatac taataggaat cgtaaaaaca gaaaaaaagc ataatgataa
    11581 agggtcgggc agattttaat tgattcatct ggtactcttt tttattgtcc ttacattcat
    11641 aaagtttgaa tgcaatgact ttattacatc tatatttata tctaacagta gcgttgatgt
    11701 gttaattata agttctatca ctttatgttc aattagtttg tgctcttata tgtgtgattg
    11761 aaaatataga taaaaaaaca atcatcggct ttgacttcga tgattgtttt ctttattttg
    11821 taaaaatcct atcacttgat tttatggtaa ccttttattt ttgtcttcaa acggcttggt
    11881 aagagagcag taagcagtgc aaatgggatc gaaaagacat accagaaaat cgtacgtccg
    11941 ataccataat gttgatagag ctttgtcaac atcacgtgta acaagatcaa ccctaaaagc
    12001 aaggagccag ctataaacca atgattatcc caatgcatga cttcactaag tgccatcact
    12061 ccaaaaggat agaaataaaa atagatcccg actaaggtca tccgccagtt ctttgtaaat
    12121 gttccgatat tcaataaaaa gaaaatcgct gaaagcaaaa taccgatagg cggaaaaagc
    12181 ccaaccaaaa aggaatacaa catcccccac aagatcatca caggaccttt gatcaatgca
    12241 acgattgcga tgaacaaagt cagcaatatt agtttttgtg ataacgttgc aacagtcatt
    12301 aatccaaata aaattaatgt gaaccaatgc caatgggact gctgttgttc tttacgggaa
    12361 aaatcgaccc ggttggataa ataatctgct aatttcggat aagaacgctt aaaatccata
    12421 aactgttcct ccttcgtgga aaaggtaggt ttagtatacg aaaaataaaa aagaaataca
    12481 tccgtcttaa gagggagttt ttcactgaaa gtggagaaaa aagaatggta aattaaaata
    12541 ttctcagtta tactgaaaat tataccaaat ttcaaagaag aaagaggatg ggaatgaaag
    12601 gcaaactaaa acgagaaatc aatctgtttg gcgcattagc taccgttatg ggaaccgtga
    12661 tcggtgcagg cgtcttcttc aaaactgctg ctgttactgc tagtactcag tctacaagtt
    12721 tgacattgct agcttggctt ttgggtggtt tcttaacgat ctgtgctgga ttgacagtgg
    12781 ctgaattagc tacagcaatc ccggaaactg gtggagctgt aaaatatatt gaagctgcgt
    12841 atggaaaatt gcccagcttt ttattaggat gggcgcaaag cttgatttac tttcctgcta
    12901 atattgctgc attatcgatt atctttgcga cacaattgac gaacttatta caattatcta
    12961 ccgattatct tttgttgatt gcagtgatca ccgctgtttc tgtgacaggg ttgaatttgt
    13021 tagggacaaa agtcggtgct tctgtacaat cagtgacgtt gattgttaaa ttaataccaa
    13081 ttgctgtgat tgtcatatgg ggattattga ccccaggaca aggaactgtc cagttatttc
    13141 caatcgaagc tggaaaagat gttacttttg tcgaaggatt gagcagtgcg ttgttagcaa
    13201 cgttgtttgc ttatgacggc tggttaggtg tcggtgcaat ggcaggggaa atgaaacggc
    13261 cggaaaaaga tttgcctaaa gcaattattc ttggtttgag ttttgtaaca gtcgtttatc
    13321 tattgatcaa ttttgttttt ttgaaaactt tgccgattga tcatcttgcc ggtaatttga
    13381 atgcagcttc tgaagcatca gacgtcattt ttggaggaat cggtggaaag ctggtcacta
    13441 ttgggatctt gatctcggta tacggtgcat tgaacggtta tacactaact gggatccgcg
    13501 tgccttatgc gatggcttta gaagacgagc tacctttcag taaacagctg acaaagcttt
    13561 caaagaagtt tacggttcct tatgttcctg cggttttcca gttagcagta gcttgtatta
    13621 tgatgagcct tggttctttc gactttttaa cagacatgtt gatttttgtg atgtggctat
    13681 tcagtttact gatttttatc ggtgtcttta ttttaagaaa aaaagcgccg gaattgccac
    13741 gtccatataa agtaccactt tatccaatcg ttccgattat tgcgattctc ggtgctattt
    13801 tcattttagg tatgacgatg atgacgcaaa cgaaattagc attgatcggt ataggtgtta
    13861 ccttgatagg tatcccagtc tattatcaaa aaaagaaaaa acgctcagaa gagtaaaata
    13921 tttcaacaac agacagaaaa agtacgaact aggtcctcta gatcttcgtg ctttttctat
    13981 ttcaataaaa caagttcttc tattttgatt tttgctactt ctgcaagtag tgcttatgtg
    14041 ctaaagtaag aatgaaaagg agagataaac atgatatttg attctcatac ccatttaaat
    14101 gctgaacagt tcaatgaaga tattccagaa acgattcagc gagcacaaga aatgggggta
    14161 acgaaaatgg cggtcgtggg cttcgatacg ccaacgattg aaaagtcttt gcaattaagc
    14221 catgactatc caaatatata cagtattatc ggctggcatc cgacagaggc tggcagttat
    14281 acgaaagaaa tcgaaaataa attacaagaa caattgactt taccaaaggt tgtagcatta
    14341 ggggaaattg gcttggatta tcattggatg gaagacccga aagaagtcca agataaagta
    14401 tttcgcagac agattgctat cgcaaaagaa atgaacctac caatcagtat tcatactaga
    14461 gaagcacttg aagacacgta tcaaatccta aaagaagaag atgttcgaga tatcggtgga
    14521 atcatgcata gtttcagtgg tgattatgaa tgggcaaaac gatttcttga cttagggatg
    14581 catatttcat ttagcggtgt cgtcactttc aaaaaagcat tagacgtcca agaggctgca
    14641 gcaaatgtac caatcgaacg attacttgtg gaaacagatg ccccttatct ggcaccagtt
    14701 ccttttagag gaaagcgaaa tgaaccaggc tatactcgtt atacagtaga aaagattgct
    14761 gaacttcgga atcttccgac agaagaagtg gctgcccaaa catggacaaa tgcgcaccga
    14821 ttatttggtc taagcgaaca tggataagat gaagatccaa gagattatag tcgttgaagg
    14881 aaaagacgat acaagaaggc tgcgggaagt agttgatgca gatacgatag aaacaatagg
    14941 gtctgcaatc aatgaagaga tcctgatgca aattgaacat gcccaagaga ctcgtggagt
    15001 gatcgttttt accgatcctg atttttcagg agaaaaaata agaaaaatca ttatggaagc
    15061 tgttcctcag gcaaaacatg cttttttgcc gcgcacacaa gctaaaccaa agaaaaaggg
    15121 aagccttggt gtcgagcatg caagtgatgc tgctatttta gaagcattga aaaaggtcgt
    15181 gacgccagac atttcccaaa cggctgtttc tgaaatcact agacaagacc ttttagacta
    15241 tggtttaata gctggggcac aagcaaaaga aaaacgagaa aaactaggtg acgagttgcg
    15301 catcggttac acgaacggca aacaattgga aaaacgtttg aagatgtttc gtattacgcc
    15361 gaaagaactc gcccaagcaa tgaaaatagt ggaggaacaa catgagtgaa tatcgtgaaa
    15421 ttgcgacacc ctctagaaca aaagagattt tattgaaaca tggtttttca tttaaaaaaa
    15481 gtttaggaca aaattttctg actgaaccaa atattttaag gaaaatcgta gaaactgcgc
    15541 aaatagatga acatacgaac gtgatcgaag ttggaccagg aatcggtgca ttgacggaac
    15601 aattagccaa gcatgcaaaa caagtcgtag catttgagat cgatgatcgt ttgatccctg
    15661 tattggctga tacgatgcaa ccctatgaca atgtgactat tgtacatcaa gatattttaa
    15721 agaccgattt gtcaacagct gttagagaaa catttcatga ggagcttccg ttaaaagtcg
    15781 tagcgaattt accttactat attacgacac cgatcatgat gcattttctt gaatcagatt
    15841 tggttgtgga tgaactagtc gtcatgatgc aaaaagaagt agctgatcgt atctctgctg
    15901 aaccaggaac gaaagcttat ggttcattat cgattgctgt tcaatattat atggaagcca
    15961 gtttagcatt catcgtacca aaaacagtat ttgtccctca gccaaatgta gattcagcga
    16021 ttttgaaact gacaagaaga gatataccgg cagtcgaagt gacagacgaa aaagcatttt
    16081 tccgattgac aaaagcggca tttcagcaaa gaagaaaaac gctttggaat aacttgcagc
    16141 attcgtatgg caaagatgac cagacaaaag catggttagc aaaaagcttg gaaacagctg
    16201 gaatcgatcc aaaacgtcgt ggggagactt tgtcattaca agaattcgcc gcattaagca
    16261 atgcgatgtc agaaaatatg cagtaaaaaa agaggttgtg acagaagttg tgaaaaagct
    16321 gtcctgcatc aagtaataag aacagccaaa caaaaatagt tcctcatgtt tttcgtttgt
    16381 ttgggcttgt taccgcagat gcaagctttt gaacaccgtt taagaaagag gttgtgggcc
    16441 tgccgtttaa ctctaagtca caaggaacaa tttcaaaaaa cagcttctca tgtttttgag
    16501 ttagttggtc ttattacttg atgcaagctt ttgaccacta ttatgccgaa caaggagatg
    16561 tgacacaaat tttgtcacat ctcctatttt tgtttcttga tcaaaaaatt cttatacttg
    16621 atactcacac tcattttgta tggatttaga aaagaatatg tgtcttgaca gttattagta
    16681 agtgagttat actaagtcga catttatgtg tcaagacata ttaggagaga gaaaaaatga
    16741 gaaaaaattc agttcgaaaa ctaacgattt ctgctttatt gattgggatg ggggtcatta
    16801 tcccaatggt catgcctaaa atcatgattc caccagcttc ctttactttg gcgtctcatg
    16861 tgccattatt tattgcgatg ttttttacac caggagttgc agtggctgtt gcgctaggta
    16921 cgacatttgg tttcttttta acaacgccgg ttatcatcgc attgcgagca ttgtcacatc
    16981 ttgtgtttgc gttgattggg gcttggtact tgcaaaagca tccgagtatc gttttaaaaa
    17041 atggacagtt tacttttgct aattggcgtt tccaagggtt caattttgtg atcggtgtga
    17101 ttcattcttt agcagaaatg ctagtagtca gcatctttta ctttattggt aatatgcctg
    17161 aaacatatta ttctcaagga tatttttata cagtattcat cttgatgggg attggtgggc
    17221 ttatccacag tttagttgat tataatattg cttattttat agcaggaaca ctcagcaaat
    17281 attttgatat ccccgtattt acggcagcaa aaaaagaaaa tcggaaagag tcgattcaag
    17341 ggtacgcgaa gaaaacgaca tattagacat gatctctcaa aaaaaaaact ctcatctagt
    17401 aatggataat aaatcctcta agatgagagt tttttgtcct aaaaagagct gcaccaacat
    17461 cttctggcac agctctaaca gggggcaatt tatggaagtt tgatgacttg ttctgtttct
    17521 ttatcaaaga agtggccttt gctgatgttg aaggcaaggt cgactatttc gcctggttta
    17581 tggaaatctc ttgcatctac ttttgagatg aactcagtag aaccagtttt tgtgtaaagc
    17641 atcgtttcag ctccaagtaa ttcggaaacg acaacttctg atttcactac ggcttcaggt
    17701 gaagcttcaa gggctacttg ttcactgtgg atgtcttctg gacgaatacc gaagataact
    17761 tcttttcctt cataaccttt ttcaactaat actttgtttt ttccttcagg aatacgaagc
    17821 ttaagcccat gtccatctgt gataacacca tttttcaatg tcacattaaa gaagttcatg
    17881 gcaggagatc caataaagcc ggcaacgaac atattgacgg gtgtatcata aacttctttc
    17941 ggtgtaccga tttgttggat aaatccgtct ttcatgataa cgattcggtc agccatagtc
    18001 attgcttctg tctgatcgtg agtaacatag atggtcgttg tttcaagacg ctggtgaagt
    18061 ttggcaatct cagcacgcat ggctacacgt aatttagcat ctaaatttga taatggctca
    18121 tccattaaga agacttttgc atcccgaacg attgcccgtc ccaatgccac acgttggcgt
    18181 tgtccaccag ataaagcagc tggttttcgt ttcaagtatt ctgtcagacc taagatctct
    18241 gctgcattgt ccacacgttt tttgatgtct gctttatcat atttacgtaa tttcaagcca
    18301 aaagccatat tatcaaaaac agtcatatga ggatacaatg cgtagttttg gaaaaccatg
    18361 gcaatatcac ggtctttagg tgccacgtca ttcatgactt ttccgccgat atttagttct
    18421 ccttcagaga tatcttccag tcccgcgatc attcgcaaag tagttgactt tccacaaccg
    18481 gaaggaccaa caaaaacgat aaattcacgg tctttgattg atagattaaa atcagttaca
    18541 gaataatttt ctgcattgtc atatttttta tatacgtttt tcagagccat ttctaccata
    18601 atacgttcca cactttcttt tttagtttta ttaactattt aaagtataaa tgaaagcgtt
    18661 aactaacttc aatgtcatgt tgaacaagaa ttttttcttt tttttgtcaa tgtgctagtt
    18721 ttaagcttta ataaatatcc ataagaaata aaaactgata cactggagta aataagtatg
    18781 ttaacaatat caaaaggagt gtctcttatg aaaatcgctt tgatcgctca tgaccgaaaa
    18841 aaacctatga tggtgaaatt agcaacagcg tataaaacaa tattggaaaa acatgaactt
    18901 tttgctaccg ggacgactgg gcagaggatc acggaagcaa ccggcttgcc gatccatcgg
    18961 tttaaatccg gaccgttagg cggggatcag caaattggag ccatgatttc ggaggatcag
    19021 ttagatttgg tgatttttct tcgagacccg ctggctgcac agccccatga accagatgta
    19081 acagcactta tccgtttatc tgatgtctat gagattccgt tagctacgaa tatcggaaca
    19141 gctgaggtgc tactaagagg actggaagcc ggcttcctag attttcgaag aatcgtccat
    19201 gaaatagaca agcatccact ttcattttga tggataagcg tatggaaaaa taacgtttaa
    19261 tgttcgccta tatgtagcta taaaagctaa acaaactgat aatatgcgat ataatacgaa
    19321 cgtattggat aatataaaca ataatattag tttttcgtat ttgcagtaaa tgtgaaaata
    19381 tgctatagtt tctgtgggaa aagatcccaa aaataataca gagtagaaaa taaagcgtta
    19441 agtgctaaga ggatgggatg ttgcctcttg gacgaaggac gtaatgttcg cggttttatt
    19501 ttcgcattcg ctgcatgata cgatgtagca gctcttaaag gagatgctaa aaatgaagaa
    19561 acaccggtta gacgcacgta tgattgctat tatgggctta ttgattgcat tgatggtcac
    19621 tttgtcccga ctggtagcaa tcgagacccc atttattaaa atcagtgtca catttattcc
    19681 acaagtgatc atgggcattc tttttggtcc tttttggtca gggataggcg cagttttagc
    19741 tgatttagta ggaatggccc tcttctcgaa atctgcattc tttattggat ttacattgaa
    19801 tgcatttatt gaaggagcaa tctacggatt ctttttctat cgaaaagaga tcacttggaa
    19861 aaatgccatt ctggctacgc ttagtgtcac gctgatcatc aacttatttt tgacaccatt
    19921 atggcttgcg ttgatgtacc atgtgccatt gttcagctgg gtcgtttggg cgccccgcct
    19981 gttaaaaaca gtaatatggt taccgattca atcaattgcc atttattacg taggacgtag
    20041 tattccttac aaaaaaattt taagaagttt agcgatccac gctaaataaa aaacaagcag
    20101 tcttcccatc ccagaagact gcttgttttt attgtgctgt gaattgtgct tgatagagcc
    20161 gtcggtaata accatctttt tgaagcagct catcatgggt gcccatttcg acaatagctc
    20221 cctgatccat cactaatatc tggtccgcat ttttgattgt ggacaggcga tgggcaatca
    20281 caaaactggt ttttccttcc atcattgcta agaaggcttg ttggattttt ttctctgtca
    20341 atgtatcgac tgagctggta gcttcatcta agatcaacat agggggatta ctgatcatcg
    20401 ttcgagcaat cgtcaacagt tggcgttgtc catctgatat ctttaaccct tgaccgccaa
    20461 tcttagtatc taatttttcc ggcaaacgtt ctacaaattc atacatataa gaggctttta
    20521 gggcatcatt gatttctgcg tcacttgctt ccggattccc aaaacgaaga ttttctcgca
    20581 atgtactgtc aaatagccaa gtatcttgta acaccattcc aaaactttta cgtaatgtat
    20641 cacgttggat agagcggatg tcatggtcat cgactgtaat tgttcctttt gttgtgtcat
    20701 aaaaacgcat cagcaaatta acaagtgtgg attttccggc ccctgttttt cctactatag
    20761 caatggtttg acctggataa gcagtaaaat tgaaatcttc gatcaaacgc tgcttggggt
    20821 cataagaaaa agaaacatga tcaaaagaga cttttccttt gatatttttg ggttccaagg
    20881 cataggcatc tggcaaatca ggcttttctt ctggctgatc gatcaaatca aatgctcgtt
    20941 ctagaccaga aaaagcggtt tgcagttgtg tcgtgattcc agaaagttcg ataaatggtt
    21001 tagaaaactg acttgagtag atcgtaaagc tggaaatcac accaacagtg attgacgaac
    21061 cactcttcaa tgccaatagg ccgccaacta aaccaatcga taaataagca aggtgatcta
    21121 caaaacgaga agacgggttt gtcaaggaag aagaaaattg tgcccgttgt cctttttcat
    21181 atagatcagc atttagcagt tcaaattgct gttgactagc ttcttcttgc tgaaaagctt
    21241 tcacgatttt ctggttaccg attcgttcgg tcacaaagcc cgagatatcc ccaataatcc
    21301 tttgctgtct atcgaaattt gtttgtgaag cacgagcaac gatccaactc acaaggaaaa
    21361 taattggtgt agaacaaaga acgaccaatg tcagaagtgg acttagacgc atcataaaaa
    21421 taaacgaaag aatgatgatt gccactccag aaaataattg attgaaggcc gcagaaattg
    21481 caatggaaat attgtccata tcatttgtaa atcgactgac gatatttccg tgggagtttt
    21541 gatcataata acgaagtggc aatcgattca gatgagcaaa tgcatctttt cttaattttg
    21601 caacggataa gtaagctaac cgattgctta acacttggat gagccattga ctgactacgt
    21661 tgattaaaac gataccaccg aatagaataa gtaaatggaa cagtcgattg aagcggacct
    21721 gtccttctcc aatcatttga tcgatacttt gaccaatttg taacgtcatc acaactgttg
    21781 caatcccatt gatgattcct agtatcacag ctagccaaat gtcttttgca taggcagtaa
    21841 ggtaaggaat gaatcggcgg agcgtctgca aggataattt ttttactttc ataactattc
    21901 ttcctcctgt gatgcgacga tttcctgata ttctgctgat tgagcaagta attcttcatg
    21961 ggtaccctga gcagcaatct taccttgatc catcactaag atatgatgtg cttcttgaat
    22021 agagcggaca cgttgggaga taataatcac tgtactgcca agataagtct gtagcgcctt
    22081 acgcaaatcc aagtctgttt gataatccaa cgcacttaaa gagtcatcta aaatcagtaa
    22141 tgctggcttg gcaatcaaag ctcttgcaat cgtcagtcgc tgtttttgtc cgccagagaa
    22201 atttttccca ttttccatga cttttgtatc taaaccattt ggaagctgac ggacaaattc
    22261 ttctgcctga gcgattttca gagcttccca acattctttt tctgtggcgt ctttcttacc
    22321 ccattgaagg ttttcacgaa tagtcccggt aaatagtaca gaagtttggg gaacaagagc
    22381 aatttgctga cggagatgat ccagctgcca agaacgaaca tccattccgt ccattataac
    22441 tgtaccggaa ctgacatcat agaaacgcgg aatcaattgt gtcaaagtcg ttttcccgct
    22501 tccggttgga ccaatgattc caaggactgt atttcgcttc agctgaaatg taatattttg
    22561 gagagaaaga ccagaatcag gagtataacg gaagtcaaca tggtcaaaag cgataattgc
    22621 ttcagaagaa aaatcgggag tagtactgac ctcagcttca agaatcgaat tttctgtgtc
    22681 tagcacctct ttcacccgat ttcctgaagc ttcggcacgt gtgaagatca cgacgaggtt
    22741 tgaaacaacg atcagtgcca aaagcatttg attcatataa ttgatcaaag caagaacttg
    22801 tccttgttgc aagctaccaa tatttacttt gatcccgcca actgtcagca aacagatgat
    22861 cccgacattc atgatcaagg ttgtagcagg tgataatagg gcagagatat tggaaacacg
    22921 taaataattg ttcgctagat catttgtgct tttatcgaac ttctctgttt ctttttttgt
    22981 ctgcgcaaat gcgcggatga cacgaacacc gctaagattc tgactaacgt gttcattgag
    23041 ctgatctagt ttttcttgca ctttttggta gagaggaacg gtttttttga tgataaaaaa
    23101 taaaatgatt gagaaaatag gcaataatgc taagaaaaac agacctactt cccagtcgat
    23161 cacaaaagcc atgaccactg acccgatact taaaaacggt gctctgatca ataaacgaat
    23221 gaccattgct aaagctagct gaagctgatt gatgtcgttt gtcattcggg tgatcaaggt
    23281 atctgttcca aaatgattca actcgttgtg agagaaagta ttgatttttt tgattagctg
    23341 attacgtaac tctgtaccaa atccttgtga agctactgaa gcatagtatt ggcaaatgat
    23401 agcacaaatc agaccaatca cagacatagc gaccatccat aagcccattt caatgatttt
    23461 tcctcggtca ttcattttta gtccattatc gatcaaggaa gccatcatta aaggcaggac
    23521 aagttcgaag acagcttcaa gaaatttgaa gaacgggccc aaaatgattt gcttacgata
    23581 gtcttttgca taacgtaata actgaatcat atgattcctc ctattattta tttgtccaca
    23641 attgaaaaaa caagtactac cacattgtag cttaggaaat ttagaatgaa aagagaacaa
    23701 gccttttcat aaatgaaagt tttattatga agaagcttta gaaattgaat agactatttt
    23761 ttttaaaaaa aagaaaatga gaaaacatgc cagtctcttc attcatagga acgtctattt
    23821 tacttaattt ttctataaat gaattctgca taaaaaatat atcaagtaaa tgtttataga
    23881 tgaaactcta tttacttctt ttttaatttg ttcttgcaat cttttttgct acgtaatttt
    23941 tgtaaaaaag tgggttgaga gaaatctaat tgattttcaa agaaggagta tgaaaatgag
    24001 aaaaatattt attcgtttat tgtgggtact atcttagagg aataaataat agtggagaaa
    24061 actatggttt ggcgcagatt ctatcaggtt tgatggaacg aagacaacct cccgacatca
    24121 agtcaaaagc tacttgagat tttgatctga aaataaaaaa agaaaacaag gtaggaacat
    24181 caatgtttct accttgtttt cgtaatcaat gggaaaaaac taagagtggt gtacgtaatc
    24241 aataaaaagg atggtgacaa cagtacctct ttactctctg aaagcatcat aactcgtttt
    24301 tcctcattat gccaataaat tgttgttata ccacatataa ggatttgtaa tagctaaaaa
    24361 cagacaggaa agttgaaatc tgtgaatctt attttgtatg gaaggattta tagaacgcaa
    24421 aaatcaagat aaagatgaat aaaaatcctg tttcggctat atattgcgta tctacgccaa
    24481 taaaacgagt ttgttctgtc atagccatat aaagcaaagg caaaaataca actacagcga
    24541 gataagatcc gatgatttct cgaaaagttt tccaggaagt atgtttcctg tttttatatt
    24601 tatccgattt cattgaatag tcatagcctc cttattcttt catatgttga aattccagta
    24661 taaaaactgg aaggaactgt acgtttttta gaataaaaat tataagggat aagtaggctg
    24721 ctgtctataa taagatatat ttttcaattc tttattaaag attttattga caaaatgaat
    24781 ccgttttcta tgatggagga ggggtgaata agttgttgaa gaaggaaatt ttatgttact
    24841 tagagaacca gacagccttt ttcgatttag aacatatgaa cgaggtcttt acagcacaaa
    24901 ggttagctga tcattttggg gtaaaaagaa acacagtcag tcattatttg aaccagttaa
    24961 atgatgagca actactagtc aaaatcaatt ctcgaccagt gatttatttc cacaaagaag
    25021 cttttgaaaa acaatttttt aagttacgca cgaattttta tttttctatc gatgatctca
    25081 aagaggaaca gccttttttc gcgcaaccaa aagacttatt ctcttttatg atcggatacg
    25141 acgccagttt gaaagaaagt atcgaacaaa tcaaaacagc tctttactat ccagatggcg
    25201 gattaccact tttgattaca ggcgaaagtg gcacaggtaa aagttattta gttaatttgg
    25261 tctatcagta ttgtctgctt catgatctgc tagaagattc tgcaccattc atcactttta
    25321 attgtgcaca atatgcagat aatcctgaac ttttgactag caatttgttt ggacatgtta
    25381 agggagcgta cactggtgca gaagaaaata aaaaaggggc ttttgaagca gcagatggtg
    25441 ggatcttgtt tttagacgag gtacatcgct taacaccaga aggtcaggaa aaactgttca
    25501 cctatcttga tcaaggtgtg atttatcgta tgggagaacc taatttgtca cgccggatca
    25561 aaacacgtct cttttttgct acgactgaag agattactag tcatttctta acaactttca
    25621 ttcgaagaat acctatccga atcgaactac ctgcacttaa tcagcgtagt cgtaatgaac
    25681 ggctagaact gatttactct ttttttctcg aagaacaaag aaaaatgggg agccctttgt
    25741 cagtcagtgg accagctttg tcacttttaa caagtcagaa attatccgga aacataggag
    25801 agctgaaaaa tatcgtcaaa gtttcagcag caaaagcata tgctgaacag cgtgaacagc
    25861 ctgaaatcca tgtcacgatc catcatttgc aaaaagaact acttgcgcag cctattacta
    25921 aaaatacagc acagcaagga atctatatta cgcaagacca gaccttagag caattaatgg
    25981 agaagcaaca gcccgaacag cagcgaatcg ttaaaagttt tgagcaaatc ctaattgatt
    26041 tcaagaaaaa taactgtctg ctatattctt gcgaaggaaa attaaagcaa gaagtgattc
    26101 aattatttga ttttttgctg tttgagacca gtagacaaga aaaacacgag ctgctggttt
    26161 attggacaca atctatccga gagacctttc gacaaatgga aactgcctat caaatcaaat
    26221 tcgatggaaa cagtgtctat gccttgagtt actatctcta tcagcgaagc actatcaaat
    26281 ggttaccgga agaacatgat attatccaac taatcaaaga attagaacaa caagtttctc
    26341 agtcttatcc atctagttat cactatgtcc aacgaattct ggaattaagt aagatcaaat
    26401 tagatattga agacacaagt atggatcgga ttttactgac gatctatcta aaaaaagcga
    26461 aatggtcaaa agaaaaacga ctgccaaaag cgatgattgc tgctcacggt tatgcaactg
    26521 ctagcagtat ggcaaatgta gtaaatcgtt tattacaaga ggatctattt gaatcatttg
    26581 atatgccact agatgtcaca ccacagcaga ttgcttcaga aatccttgat tattgcgaaa
    26641 acagcgatgt ttcaaatgga ttgatcatat tagtagacat gggatctctg aaagaaatcc
    26701 atcagttttt taaaaaacag ttatccgtac cgcttttgat tttgaataat gtgacgacac
    26761 ctttagcaat cacagtaggg gaatgcctgc aaaaaaatgt atcattagaa gagatagcag
    26821 aagagactgt tcgacagatc cagccagaat ggcgactgct ctatccagag gaaaacaaac
    26881 ccaaagcact cattactact tgcttcactg gaatcggtac ggcggtccac ttaagtgaac
    26941 tgctagaaaa aagcttacct acggcttgtc agttgaaaat catcccatat gaatatcagc
    27001 aattaaaaga taagaaaaac agtgacccat tattttccat atacgaagtc gtaggaatga
    27061 ttggcaccac agatcctgat atcaccaata tcgactatct ttcattagaa gacctcatct
    27121 cgggggaaaa gatgtctgta ctagctgaat ggttggacac tactatgaat gcttcagaga
    27181 aggaaatatt caatcagcgc atcattcgta atttttcatt ggaaaaagta ctggattccg
    27241 tgactattct agatacagag aaggtgatgg gagaaatcga tcattttatg agagaattag
    27301 aggtgcagtt gaatcgaagc atcagcaatc ccaaaaaatt agcgttgtat gtccatgtga
    27361 gctgtctcat tgaacgattg atccgacaaa tgccaatcga aacctatcaa ggattagaga
    27421 aatggaagca gtgtcaaaaa gaagggttat ctgctattaa atctgcgttt agtgtcattg
    27481 aagagcatta tagtgtcatg atccccaatt ctgagattgc ctatatctat gatattttga
    27541 gtgaagatac agagtttctc tcgatagaag aagaattttg aacataagca agttgttgcc
    27601 actaattttg gcatgcaact tgctttttta gtatgtgtaa gtgaaaagag gagatgaaaa
    27661 agtggataga gaaattatct tagcttccca tggaaaattt gcttcaggga tattagattc
    27721 attagagctg atctatggga aaaatcattc tattactgta ttcgattgct atactgatga
    27781 gaattttgat ttagcggaaa cagtaaggca gctttttatg aggtacaaag ataaagaaat
    27841 cattgttctg acggatcttt ttggcggaag tgtcaacaac gaattcttac aatatatcaa
    27901 tcgggatcat gtctatctcg ttgccggatt gaatttgcca ctattgattg agctagtatc
    27961 tcggttggac gctgaagtag agacaactgt tttgatccag caggtgctaa ctgattcaaa
    28021 aaaaatgatc cagttttgta acgagcggct caatcagaaa atagaagaag acgaatttta
    28081 ggaggaagaa aaatgattgt attgacaaga gtcgaccatc ggttattaca cggacaagta
    28141 gctttttcat ggacacaaac aataggcgca gactgtattt tgattgctaa tgatgatgta
    28201 cctacgaatg agatacgaaa gacaacgatc aaattagcca agccacaagg agtcaaacta
    28261 gtgatcaaat caattgatga ctcgattgcc gcgttgaaaa gcggggtgac agataaatat
    28321 aagttattca tcgtagtgga atcgatcgag gatgcatata agctggcaga agcgtatcca
    28381 gaaatcaaaa ccatcaactt aggagggatc aaggcaaaac ctggtactcg cagcatcgga
    28441 aaagcggtaa atattttacc agaagaggag gaacgcttga aacgattaat tgacaaacaa
    28501 atagaagtag aaatccgtca agtaccagca gataaaaaag tggatgtaag agaagttttg
    28561 taagaaagga agaacctcaa tgattgtaca agcagtctta ttaggaattg ttgcatttgt
    28621 cgcacaatca gaatatgcac tagggacatc gttgctttct cgtccgattg tgacggggct
    28681 gtttacagga atcgtgttag gtgatgtaaa aactgggatc attatgggcg caactttaga
    28741 gttagcattt atcggatcgt tctcagtagg tgcttcgatt ccacctgatg ttgtgaccgg
    28801 tgggatttta gggacggctt ttgctattac agctggagcc ggtacagaaa ccgcgctttt
    28861 actaggtttg ccgattgcta cgttgacatt gatattgaaa aatatttact tagggctttt
    28921 gattccaatt atgaaccata aagcagacaa ttatgcagaa gaaggaaatt ataaaggagt
    28981 cgagcatatg cacttgcttg ccggtatcgg tctttccttg atgcttggtg ttgtggtaat
    29041 ggtttcttat atggtgggaa gcaatacgat cggaaatcta ttaaatatga ttcctgactt
    29101 cgttcaaaag gggttgtcag tagctacagg gttgattcca gctcttggtt ttgcgatgct
    29161 tgctcgtttg ctgatcaaca aacaagttgc accatatttt ttcttaggtt ttgctatagc
    29221 ttcttactta gagattcctg tgaccggagt agctattttt ggtgcgattt tagcagttgt
    29281 ggtagtcaat attatgaatg tccgtcaact accacttcaa acaactgcag gggaggtaga
    29341 agaagatgaa gaattctgag atcaaaaaca gcaaaataac gaaaaaagag ctaaatcacg
    29401 tattttggcg ttcttttcaa atggagtttt cttggaatta tgaacgtcag atgaatatgg
    29461 cttatgttta tgcaatgatt ccgattttga aaaagttata tcagtcgaaa gaagaaatgg
    29521 cagcagcttt gaaacggcat ctagagtttt ttaacacgac cccacatatc gttacactga
    29581 ttttaggaat cacggctgca atggaggaag aaaataagga tgatcctgaa tttgatgtga
    29641 ctgcgatcga tagtatcaaa acatctttga tgggacctct tgcagggtta ggagattctt
    29701 tcttttgggg aactttgcga ttgattgcca caggtgtggg tacctcttta gccatgcaag
    29761 gaaatatttt aggccctatc ttgttcattt tgattttcaa tattccgcac attctgtttc
    29821 gctatctagc aacaggttgg ggatatcgct taggaacggg ttttttgaaa aagatccagg
    29881 caaacggtat gatggaaagt ttgacattag gcgcgtctat tattggactg atggtcgttg
    29941 gagcaatgac tgccacgatg atcaccatca atattccttt aaaaatcggt tcaggagaaa
    30001 atgctgtgac tgttcaaagt atttttgatg atatcgttcc aaatatctta tgtcttggag
    30061 catttggcat agtgttttat ctattgaaga aagaagtgaa accattaacg atcttgatcg
    30121 gactagcttt ctttggaatt tttggttcgt ggatagggat tttttaaaaa aggagcagag
    30181 aaaatgttta cgatgcaaga ttatatctat gaagaaaagg aagtactaag tacgatcttg
    30241 aaaaagaatg atttttcgac aagagaacat atgaaaaaaa cagtgaattt actgatctta
    30301 gcaacgggtt catcttataa cgcttgttta gcagcgaagc cagcattgga atcttatggt
    30361 gatcttactg tcgatattca agaacctttt tattttttca attatggaaa actttctcct
    30421 tcaatcgata ctgtgattgc tgtgtcacaa agcgggaaaa gtgcttctac tgttgaagca
    30481 gtaaaaatga tacaaaaaca agggttacct gttgttgcta tcacaaatga cgtgcaaagt
    30541 ccacttgctt tagaagctgc acaaattatt gatttaggtg ccggggtaga atcagtaggc
    30601 ttcgttacaa aaggctattc tgcgactgtt ttacagttac tactcttagg gataggaatt
    30661 ggaataagta aaaataaaat cagcaaaaaa atagaacaag actacatgca acagttgcgc
    30721 aaaataatca accatttacc agcgattatt caaaaaacgg aagaattttt tgatggatat
    30781 caaagtttat ttcgtttagc acagcgtttc gtcaccattg gatatggtcc aaactgggga
    30841 acagctaaag aagcagagac gaaattgact gaaacaatcc gcgttccgtc acaaggattt
    30901 gagttagaag cttatatgca cggcccatac ctggaggcag atgcttcgca tttgctattc
    30961 tttattgaag gggaaagtgt aaataaggaa cgttcgcaaa agttgcaaag atatatgagt
    31021 cggtacgttg gagagactct caccattacc acaaaaaaag caagaaacga gaaaacactt
    31081 ggattagcaa tagaatgtga tgaatatctg tctgtattag cgcttgtcgt tccgtttcaa
    31141 ctattcgctt ataaaaccgc agtagctaaa ggtattgact tgaacaaaaa gatttttgaa
    31201 gactttgata cagtcttgaa aagtaaatta taagaggtgg aaatcatgct gaaattcaat
    31261 gaagaagaac aaatcaaagc aataaaaggc gcactagctt tacgtccgca agtagaagaa
    31321 atcatcgata aactttatgc ggaaaaattt gacgctgtct attatctagg aatcggtgga
    31381 acgtatgctt cctcgatgca agctgtcact tatatgaatg ggaaaagcaa cttgcctgtc
    31441 tttgtacagc atgctgcaga atattacact actggaaaca aaagattaac aaaagattct
    31501 atcgttattt tgtcctctgt taccggtaca actcaagaag tagtgaaagc agtagaacaa
    31561 atcaagaaag taggagcgac actgattgga tttatcgata aagcgaacag taagttgtct
    31621 cagctatgcg attttgtggt cacttatcca gctcctggaa cagaacaaat caaatttttt
    31681 atggcagcag atcgattgat gtatttgaat ggagaattcg aagcttattc agaatactat
    31741 gaacagctcg aacgttattt accaacaggg ttagtggaag cagagaaaaa agcagatgca
    31801 tttggcttat catttgcaga aaaacatcga cgtgatagta tgcattattt tattggagca
    31861 gggaatcaat ggggagctgt ttattcatat gccatgtgtt actgggaaga acaaagctgg
    31921 cttccatcga aatctatcca tgctgccgag tttcttcacg ggacgttaga gatcgtagaa
    31981 gaaacaaccc cagtcacatt atttttaggc gaagatgaac agagagtgtt atctgaacgt
    32041 gtggctaaac ttttgccaaa aatttgcagc aattatacat tgatagacac aaaagactat
    32101 ccagtagaag gtatcagtga aaaatatcgc ggacgtgttt tgtctttctt attgatgcat
    32161 gtagtgacac aacgcatcga tgctcatgtg gagcagttga actgccatcc attagagata
    32221 cgccgctact atcgtcagtt tgattattag gatagactgg ataaaaagaa tataaaaaat
    32281 aaagagagca gcgataactg ctccctttat ttttttaaaa tattggcatc aattacgtta
    32341 taaaacacat tttttgcatt atctacagtt caacttataa tctgtttaat gggacatgtg
    32401 ggtgccaacc attttttgtt atgcaatagt actatttagt tgttgcatgg atccgtagta
    32461 aaagtccaag tacaacgagt tgcgccagtt tacatagcgt tttgtatcca gaatgttgac
    32521 taaaacaaaa gttcctatgt caaccgtcaa cagagacaat ctgtccgttg caggacctga
    32581 cagatttaga aatgttcaat ctgtctctta tgtattttaa atcaatactc actaggagaa
    32641 gtttcctaac ctcctaatag taatgctggt agtaacattg tcaaaactgc ttcctcttag
    32701 gaacaagcaa agcataacga gtgtaaaaat aaaatgtaga ctattgtaac atatcattga
    32761 ttatttcaag taaaaacgat aaaagaagta aacaaacact taaaaacaat ggaaattagt
    32821 cccaacttta agaaatgtat actataatgg aaattatcaa ttatttattt ggaggaatat
    32881 ccatgaaaca aaaaatcgca gttcttggtc ctggatcttg gggcactgct ttagcacaaa
    32941 cattggcaga aaacgggcat gatgtccgta tttggggaaa tgtacctgaa cagattgatg
    33001 aaatcaataa gtatcataca aatcagcatt ttttacctga tttgacgatc ccagaatcga
    33061 ttatcggata taaagaattg gcagaagcag tggataacgc agatgctatt ttatttgttg
    33121 ttccaacaaa agcgattcgt tcagtcgcac aggaacttat tggtaaaatg aatactaagc
    33181 caatcattat acatgcaagt aaaggactag aacaagatac gcataaacga atctcagaag
    33241 ttttggctga agaaattcca gaagaaaaaa gacaggcaat tgttgtatta tctggtccta
    33301 gccatgctga ggaagtggca gtacatgata tcacaacaat tacagctgca agtattgatc
    33361 aaaaagcagc agcctatgta caaaaactgt tcatgaatga ttatttcagg atttatacaa
    33421 ataatgatgt gattggcgta gaaactggtg cagcactgaa aaatatcatt gcaattggtg
    33481 ccggcgctat ccatggatta ggattcggag atgatgcaaa agcagcaatc atgactcgag
    33541 gattggcaga aatcagccgt cttggtgtcg cgatgggtgc caatcctctg acatttatcg
    33601 gtttgagtgg tgtgggggac ttgatcgtta cttgtacaag tgttcattct cgcaattggc
    33661 gtgccggtaa cttgttagga cagggacata agctggaaga agttctagaa aacatgggaa
    33721 tggtcgttga gggtgtggca acgacaaaag cagcagtaga gctagctcaa cagctaggtg
    33781 ttgagatgcc aattactcaa acaatctata atgttttgta taatggagaa gatatcaaaa
    33841 aagcagcaaa agaaatcatg ttacgtgacg gaaaaatgga gaatgaattt tctcttcgtt
    33901 aaatagatga tcaatagtta atcgt
//
LOCUS       DASJPC010000024        33737 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJPC010000024 DASJPC010000000
VERSION     DASJPC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.024"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            214..498
                     /locus_tag="U4A13_001078"
     CDS             214..498
                     /locus_tag="U4A13_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN1779392.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="U4A13_001079"
     CDS             complement(779..1198)
                     /locus_tag="U4A13_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN1779393.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="U4A13_001080"
     CDS             complement(1200..1784)
                     /locus_tag="U4A13_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN1779394.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="U4A13_001081"
     CDS             complement(1831..3306)
                     /locus_tag="U4A13_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN1779395.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="U4A13_001082"
     CDS             3487..3798
                     /locus_tag="U4A13_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779396.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="U4A13_001083"
     CDS             complement(3913..6249)
                     /locus_tag="U4A13_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1779397.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="U4A13_001084"
     CDS             6391..7059
                     /locus_tag="U4A13_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1779398.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="U4A13_001085"
     CDS             7062..8105
                     /locus_tag="U4A13_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1779399.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="U4A13_001086"
     CDS             complement(8342..9223)
                     /locus_tag="U4A13_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1779400.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="U4A13_001087"
     CDS             9382..10110
                     /locus_tag="U4A13_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN1779401.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="U4A13_001088"
     CDS             10266..10583
                     /locus_tag="U4A13_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1779402.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="U4A13_001089"
     CDS             10576..11184
                     /locus_tag="U4A13_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN1779403.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="U4A13_001090"
     CDS             11239..12030
                     /locus_tag="U4A13_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN1779404.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12680
                     /locus_tag="U4A13_001091"
     CDS             12168..12680
                     /locus_tag="U4A13_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN1779405.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="U4A13_001092"
     CDS             12706..13578
                     /locus_tag="U4A13_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN1779406.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="U4A13_001093"
     CDS             13754..14116
                     /locus_tag="U4A13_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1779407.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="U4A13_001094"
     CDS             14125..14877
                     /locus_tag="U4A13_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779408.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="U4A13_001095"
     CDS             14891..16693
                     /locus_tag="U4A13_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779409.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="U4A13_001096"
     CDS             16970..18010
                     /locus_tag="U4A13_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1779410.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="U4A13_001097"
     CDS             complement(18062..20206)
                     /locus_tag="U4A13_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN1779411.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="U4A13_001098"
     CDS             complement(20563..21936)
                     /locus_tag="U4A13_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1779412.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="U4A13_001099"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="U4A13_001099"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN1779413.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="U4A13_001100"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="U4A13_001100"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN1779414.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="U4A13_001101"
     CDS             complement(23384..24397)
                     /locus_tag="U4A13_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1779415.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="U4A13_001102"
     CDS             complement(24478..25077)
                     /locus_tag="U4A13_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1779416.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="U4A13_001103"
     CDS             25263..25964
                     /locus_tag="U4A13_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779417.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="U4A13_001104"
     CDS             25974..28685
                     /locus_tag="U4A13_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779418.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="U4A13_001105"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="U4A13_001105"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN1779419.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="U4A13_001106"
     CDS             complement(30770..31951)
                     /locus_tag="U4A13_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1779420.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="U4A13_001107"
     CDS             32203..33726
                     /locus_tag="U4A13_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1779421.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10691 a         6051 c         6863 g        10132 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaaa
//
LOCUS       DASJPC010000025        33011 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJPC010000025 DASJPC010000000
VERSION     DASJPC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.025"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(184..870)
                     /locus_tag="U4A13_001108"
                     /pseudo
     CDS             complement(184..870)
                     /locus_tag="U4A13_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(1006..1200)
                     /locus_tag="U4A13_001109"
     CDS             complement(1006..1200)
                     /locus_tag="U4A13_001109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779422.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(1250..1690)
                     /locus_tag="U4A13_001110"
     CDS             complement(1250..1690)
                     /locus_tag="U4A13_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN1779423.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(1694..2539)
                     /locus_tag="U4A13_001111"
     CDS             complement(1694..2539)
                     /locus_tag="U4A13_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN1779424.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            2688..4106
                     /gene="pepV"
                     /locus_tag="U4A13_001112"
     CDS             2688..4106
                     /gene="pepV"
                     /locus_tag="U4A13_001112"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN1779425.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(4163..5110)
                     /locus_tag="U4A13_001113"
     CDS             complement(4163..5110)
                     /locus_tag="U4A13_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN1779426.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(5333..5683)
                     /locus_tag="U4A13_001114"
     CDS             complement(5333..5683)
                     /locus_tag="U4A13_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1779427.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            5883..6962
                     /gene="pepA"
                     /locus_tag="U4A13_001115"
     CDS             5883..6962
                     /gene="pepA"
                     /locus_tag="U4A13_001115"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN1779428.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7106..7426
                     /locus_tag="U4A13_001116"
     CDS             7106..7426
                     /locus_tag="U4A13_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1779429.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            7448..7912
                     /locus_tag="U4A13_001117"
     CDS             7448..7912
                     /locus_tag="U4A13_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1779430.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            8117..8722
                     /locus_tag="U4A13_001118"
     CDS             8117..8722
                     /locus_tag="U4A13_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN1779431.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(8761..10050)
                     /locus_tag="U4A13_001119"
     CDS             complement(8761..10050)
                     /locus_tag="U4A13_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN1779432.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            10478..10957
                     /gene="rlmH"
                     /locus_tag="U4A13_001120"
     CDS             10478..10957
                     /gene="rlmH"
                     /locus_tag="U4A13_001120"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN1779433.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            11160..11710
                     /locus_tag="U4A13_001121"
                     /pseudo
     CDS             11160..11710
                     /locus_tag="U4A13_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            11781..12950
                     /locus_tag="U4A13_001122"
     CDS             11781..12950
                     /locus_tag="U4A13_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEN1779434.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            13423..13977
                     /locus_tag="U4A13_001123"
     CDS             13423..13977
                     /locus_tag="U4A13_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1779435.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(14150..14623)
                     /locus_tag="U4A13_001124"
     CDS             complement(14150..14623)
                     /locus_tag="U4A13_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1779436.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            14635..15171
                     /locus_tag="U4A13_001125"
     CDS             14635..15171
                     /locus_tag="U4A13_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1779437.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            15211..15582
                     /locus_tag="U4A13_001126"
     CDS             15211..15582
                     /locus_tag="U4A13_001126"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1779438.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            15866..17137
                     /locus_tag="U4A13_001127"
     CDS             15866..17137
                     /locus_tag="U4A13_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN1779439.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            17382..18407
                     /locus_tag="U4A13_001128"
     CDS             17382..18407
                     /locus_tag="U4A13_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1779440.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            18701..19810
                     /locus_tag="U4A13_001129"
     CDS             18701..19810
                     /locus_tag="U4A13_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN1779441.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            19893..20789
                     /locus_tag="U4A13_001130"
                     /pseudo
     CDS             19893..20789
                     /locus_tag="U4A13_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            20805..21629
                     /locus_tag="U4A13_001131"
     CDS             20805..21629
                     /locus_tag="U4A13_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1779442.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            21661..22971
                     /locus_tag="U4A13_001132"
     CDS             21661..22971
                     /locus_tag="U4A13_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN1779443.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            23119..24378
                     /locus_tag="U4A13_001133"
     CDS             23119..24378
                     /locus_tag="U4A13_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1779444.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            24388..25260
                     /locus_tag="U4A13_001134"
     CDS             24388..25260
                     /locus_tag="U4A13_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1779445.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            25272..26102
                     /locus_tag="U4A13_001135"
     CDS             25272..26102
                     /locus_tag="U4A13_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1779446.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            26142..28325
                     /locus_tag="U4A13_001136"
     CDS             26142..28325
                     /locus_tag="U4A13_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1779447.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            28386..29606
                     /locus_tag="U4A13_001137"
     CDS             28386..29606
                     /locus_tag="U4A13_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEN1779448.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            29603..31063
                     /gene="gtfA"
                     /locus_tag="U4A13_001138"
     CDS             29603..31063
                     /gene="gtfA"
                     /locus_tag="U4A13_001138"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN1779449.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            31140..32246
                     /locus_tag="U4A13_001139"
                     /pseudo
     CDS             31140..32246
                     /locus_tag="U4A13_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            32568..32777
                     /locus_tag="U4A13_001140"
     CDS             32568..32777
                     /locus_tag="U4A13_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1779450.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            32770..32919
                     /locus_tag="U4A13_001141"
     CDS             32770..32919
                     /locus_tag="U4A13_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN1779451.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT        11046 a         5358 c         6384 g        10223 t
ORIGIN      
        1 gaagctattt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
       61 tcaataaacg gtgtgcaatc agctgaccct cggtattagc tcaaaaaaaa cggcaaaata
      121 tgaggaacta ttttgccgtt tctttctcta atactcaggt caaaacgctg attccaaaac
      181 ctcttatttg atgggtctgt cctgaatatc ttcaaaataa tcggggtaat tatgaatcac
      241 gtattcttgc tcctcgataa tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc
      301 aaagttatga tcaatcattg attgaagcat tgtctgatgt tcatctaata ctcgacccca
      361 gtctaattct gaaatcgtca aacgtaacca tctgaaacgg ttcaagtgcg tactataact
      421 ttctagccaa tcccaaattt ctcgttttcc agcaatttca taacaagttc ggtgaaaatc
      481 agtatccaaa tcaaaaaaag cttttttatt ttgagcgatt actgcctgat cggtttgttc
      541 tagtattttt tctagtttag cttgatctta cttggtcatt ttcgcactgc attcttgcag
      601 gatcggtttt tccaactttt cccgcgtata acgggctaat gaagcagatc ggatatcgat
      661 ttttgaaaca taagtgccgg actgtggaat ggtttttaca agctcttgat tgcgcagttg
      721 gatcagtgct tctcgaatcg gtgtacgccc gatagctagt tgttcttcta acactttatc
      781 ggagatttta cttccaggtg ctaactcgga ataaatgatt tgtttacgta tagattcata
      841 agcttgctgt tgcaaattat tgataaccat ttaaccttcc tttctatgca gatataatct
      901 attataacat aaatcgctta caaatcgtgt gggcataaaa acacagtctg caaaaaaagc
      961 cctgataggc atgcctatta gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac
     1021 ctcttggatc ggtttgacac gaccgccgat tcttttagca aaagcttcgg attctttttt
     1081 gtctttgaaa atgagcagat tgtctaagtt tttttcccga gtgcgacagc cagttttatc
     1141 gatgtaaccg tgcattactt taacgacata catggggatc acctgctttt ttcttttcat
     1201 tatacgaact actcgacaca atacaagaaa aatgcttaga cggtctttat taagaattta
     1261 catcatccaa agaagcgtac gcttggaata actgatctaa ctccggattg actttgctca
     1321 gcttcactaa tcgttgcgaa tcagaaagta aaaaacagtg tttactactg ccagtagttt
     1381 ttaatttttt gtcttcagtt cccacaattt cgtagctaaa atccaaccgt gtccccgtat
     1441 acttttggat agttggatgg atgctcactt tttctccgta atggatcatt tctttatagc
     1501 tgcaatttac ttccagaacg gggatgatga tcccagattc ttcgattttt ttatagggaa
     1561 accctagatg taccaataag gctacccgag cctcttcaaa ccagcggata taattgctgt
     1621 gatgaataat ccccatttga tctgtttcat aataaaaagg ttctcgcaca taacctgtaa
     1681 atttctccat gactcatgcc tcgttttcca ttgatttttt acaaaaataa ttcatccatt
     1741 tcggaagtgc ttcgctgatt tgtgtcggca aaacaatata ttgttctttt gccagctggt
     1801 ctccaatgaa actatgaaga taaatagctg ctgcaatcgt ttggtcattt tgaggaaact
     1861 gggctaaaaa tcctgtgatc atcccagcta aagtgtcacc catgccgccc gttgccatag
     1921 cagcagaacc taaaggattg gtgtagaagt gctggtgacc ataaattttt gtctcatgac
     1981 ttttcaaaac gagcacagca ccgatttcct gttgcttctt tttgttttct ttatcccgtt
     2041 gttcttgtat cggtagagaa ctcaaccgtt gccactccat ttgatgaggt gtaagtacag
     2101 tttgttcggg atagggtagc aaaagatgat tagcagcaag taaacttaaa gcagagccat
     2161 caatcaccag ccattgttct tttgattggt tttctagtac ttgtttgaaa cgttccaaac
     2221 tcttagaaga agtacccaat ccgggaccaa tcaagagtac atctgcttgc tttattacgg
     2281 aaagaaactg gtcagtatcc gtccaaacta caatcattgc ttctggcaga tgactgtgaa
     2341 gagcactccg atttttttca tcagttacta cagtcgtgag acccgctccg ctgtgtacgg
     2401 cagctaaagc actcataatg attgctccgc catattgttc attgccgcca atcaaaacga
     2461 tacgtccaaa tgttccttta tgtgaaattt ttggacgagg tgtgatgact cttttcacta
     2521 gttgttcgtt tatcagcatc taaaaacctc cttttactca ttatagcgta tatttttttt
     2581 agagaaaaac agataagaaa aggaattgtc agattttttg aactattttt ttctgtaatt
     2641 tgtggtatca tgaacacgaa gacttaaata agtggaggat taactttatg acaattgatt
     2701 ggcaaaaaga agtagaagca cgtaaagaag acttgcttga agacttgaaa aatcttttac
     2761 gtgtcaacag cgagcgcgat gactcaaaag taacaccaga tgcaccattt ggacctggac
     2821 ctcgcgacgc attgaaacat atgttagctt atggcgaacg tgatggattt aaagtaaaaa
     2881 atgtcgataa ctatgcaggg catattgatc ttggcgaagg agacgaaacg ctagggatat
     2941 ttggacacat ggatgtcgtt cctgcaggag atggctggga cacagatcct tatgaaccag
     3001 taatcaagga tggaaagatc tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg
     3061 cagcttacta tgcgatgaag atcattaaag aacttgactt gaaactatca aaaaaagttc
     3121 gcttcgtagt cggcagcgat gaagaaagcg gctggggcga tatggcttat tatttcgaac
     3181 atgaagaaga accagatttt ggtttctcac cagatgctga attcccaatc atcaatggtg
     3241 aaaaaggaaa tgtttcttta gctttacgct tcaaaggcga caatgcagga gattatgtct
     3301 tgaaatcttt tgtttccgga cttcgcgaaa atatggtgcc aggaacagca actgcagcac
     3361 ttcaagtacc aagtgcagat gcagctatcg cgatggaaga agcattttac caattcatcg
     3421 aagcaaatcc agtaagcgga acgattgaag cggacaatac gtatgtaaaa atcgaattag
     3481 ttggtaaagg cgcacatggt gcaagcccac aatctggtat caatgcaggt tctttcttgg
     3541 cattgttctt agataactat gaattcctag gatctgctaa acaatttatc catgtagcag
     3601 ctgcttatgt ccatgaagat ttttatggag aaaaactagg agtcgcttat gaagatgaaa
     3661 aaatgggcaa attgacaatg aatgctggac tatttgcttt tgaagaaaat ggtacagagg
     3721 aagcgaactt catcaatatg aatttccgtt ttccaaaagg tgtaacagta gacggattgc
     3781 aaagtgacat cgaacaaaca gtggggcaag aaggcgcaac agttactcga ggtgctcgcg
     3841 taatggaacc acattacgta ccaatggatg atccattggt ggctacatta ttgcaagtct
     3901 atgaagacca cactggagaa aaaggctacg aacaaatcat cggtggcgga acttacggac
     3961 gtttgttgaa acgtggcgtt gcttatggag cgatgttccc aggatatact gatacaatgc
     4021 atcaagcaaa cgaatttatg agtcttgatg acttgttcag agcaacagca atctatgcag
     4081 atgccatcta tcgtttggcg aaataattaa ttaaaaagag gatcaagaat tgcaaattat
     4141 ttttgcagtc ttgatccttt ttttactttc tactgtggta ttcatgataa acatgatata
     4201 gattcaattc atattctttc ttagcgttcg tgacgactgt atccaaaatc aaccctgtta
     4261 ctaatgaaag aagtgctaaa gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg
     4321 taataaagct tgtcagcata tattcgcgaa tcacaggaat ccccgtgacc aatccaaata
     4381 aaaagaagaa gatggtccaa atcccaaaga aaagcaaagg cttataatct ttaaacattt
     4441 taataatcat catgatcact ttgaatccgt cagagaatgt attcaatttt gattcgctgc
     4501 cttcaggacg atcacgataa tcgattggga tttcaacgaa cttgaatttt ttatctaaag
     4561 catggatcgt caattccgtt tcgatttgaa aaccagaact cataatagga aaacttttga
     4621 caaagatacg attgaatcct cgatagccgg tcatgacatc aagaattttt gttttataca
     4681 ttctagtaat tgtatttcta acaaggttat ttccaaaatc gtggaaaggt ctcttattct
     4741 catcaaagta ggttccattt gataggcgat caccaatgac catatctgct tcaccagaac
     4801 gtaatttctc gagcaaacca tgtacagctt ctgcaggata agtatcatcg ccatctacca
     4861 ttaaataata gtcggcatcg atatcaaaga acatttggcg gatcacattt ccttttcctt
     4921 gacgaggttc tttttttacg attgctccac cttgaacggc caattcgtat gttcgatcgg
     4981 tcgaattatt atcatagaca taaatatctg cttctggtaa ttctcgttta aagtctgcaa
     5041 tgactgttga aattgttgct tcctcattgt aacaaggaat cagaacagca attttttctt
     5101 gttgttccat ttttcactct ccttaactat agaaaacaaa agctaaatta aaaatacctc
     5161 ataatcaaag gaaatgctag tttttcccgc taaatatcat ttatttaaaa agaaaatact
     5221 ggaatatatg tttggaaagc tctatctaaa aaaccaccca ctacaaaaga aggacatgat
     5281 acctctcttt tgaatagtgg gtgcgtattc gttctctcac ttttttgacg tattaatctg
     5341 ttgcaccttt ttttcttgaa atttctaata cggttcctgt atgagcatct gctaaaaatt
     5401 cataataaac agtttcgcca tcttctattc gagaaagacc tccccgataa gcactcgacc
     5461 gaacggcgaa tttcctaata ggttcctttt caaaagagat ccaagaacct tcgattgcac
     5521 cttcttttag aaaagatgac ttgatatttt caagtacttg gtctgctgac aatacttttt
     5581 tgcgatgata ccaaatcgtt gaagctaccc cgctcaataa tcctagaccg attccaactg
     5641 ctaacccgcc tttaaaatag cttagttcgc tttcttcata catgtggaca tacctccttc
     5701 ttctcacttg ttactccttg tgcttatttt accacactct catattttct ttgaaataaa
     5761 taaccgctac cagaaaaaat aagctataat aaatacgatc ggtctgcaga aaaaatcata
     5821 ttttatccgc agatcaatat aagtctgaga ggagtttagt ctgaaattta gacgaatagc
     5881 atatggaaga aaaaacattt caacgaatca aagagttaac agaattacaa ggaacaagcg
     5941 gttttgaaca agatgttcgc gcttatatgg aaagtcatat ggaaccgctg gtagatgagt
     6001 tacagttaga tggtttgggc ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac
     6061 cccgtgtaat ggtcgcagca catatggatg aagtcggatt catgctgaca caaatccaag
     6121 aaaacgggct ttttaaagtc gtacctctag gcggctggaa tccttacgtt gtttctgctc
     6181 agcgttttac acttaaaacc tctactggta agaattatcc atgtatttct tcttccgttc
     6241 ccccacattt attgcgagga acaagcggac aaaaatcagt agaagtaacc gatattttgt
     6301 tcgatgctgg gtttgaatca agagaagagg caatgagtta cggtgtcctt ccaggagaca
     6361 caatcgttcc ttacgctgaa acgatcaaaa cagccaacgg aaagaatatt atcagtaaat
     6421 catgggacaa ccgctatggc tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg
     6481 aaacattagg tcatacattg attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg
     6541 gttctaaagc ttctgtcaat aaattcaagc cagatctgtt ctttgcagta gactgctctg
     6601 cagcagatga tacagtgacg aaaaatggca cattcggaca tttaggagaa ggtacattga
     6661 tgcgtatcca agatccaggt ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata
     6721 tcgctgaaac aaacaatatc ccttatcaat actttgtttc aaaaggcggt acagatgcag
     6781 gtgctgctca tacacaaaac gaaggaatcc caagtacagt gattggcgta gtgggacgtt
     6841 atatccatac gcatcaaacg atgttcagta ttcgcgattt tgaagctgca cgagaaatgt
     6901 tgattcaaac actgaaaggt ttggataaat caacagtaaa tacgatcgta tacggaaaat
     6961 aatgcgatag tgaaactata aaatctggca aaagattgct ttttgccaga ttttttttat
     7021 aaaaagagga tcaatgtttg ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac
     7081 ataaacagaa agtagaggac aaagaatgat tatcccaaat agtttagaag aattagcagg
     7141 ctacgtagaa actggaaaga gtgtattttt ctttactgcc ggctggtgcg gagactgccg
     7201 ttttatcaaa ccgcaaatgc ctgaaattga aaatgacttt tctgattggc aatttattga
     7261 agtagaccgt gacaagtaca tagatgtagc cgctgaatgg aacatttttg gtatcccaag
     7321 ttttgtcgtg atccaagacg gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac
     7381 aaaacaggaa atcgaatcgt tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg
     7441 ggtcaagatg aatcaagaat ataagaatat tttagtaggt atcgacggta gtgaacaagc
     7501 acttgctgct ttcaaaaaag cggtagaagt ggctcgccgt aataatggga aagtatatgt
     7561 agcaaacgta atcgatcagc aattttataa ctttatgggc tatgcaccta ttgatcaaaa
     7621 tctagtcgat cagcaaactg aaacagccaa ggaaatgatc gaagaatgta aagaatatgg
     7681 gaaatctgtt gattacacaa atatagaagg aattattgca tatggttctg ttcgtgaatc
     7741 catggcagtc aaattgccaa aaaaatatga gatcgatcta atcatggttg ggcagtcagg
     7801 tctaaatgca gtcgaacgct ttatgacagg aagtgtagcc agctacgtga tacgtcattc
     7861 accttgtgat gtcttgatcg tttctgacga agaagaacag ccaagtaatt aagcaagtgg
     7921 aacttgtacg acctttttaa tcaattaact aaaacagtaa agggactgtg acaaaagtct
     7981 tgtcacagtc ccttgttacg aataaatggt gttccagaag aggcttcttc ggaaataaag
     8041 catgaaaaaa atttcatgca gtgaatgcaa ttttttgtta agataaatct cagcaaagga
     8101 taatggagga aagaacatga tttttgctta taacaaagca cacgtcggag acaccttgat
     8161 ggttattata gccgacgata aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc
     8221 tcggatcaaa gatgaacaag gtcaagttgt tgcttggaac tttttccata tctccgatca
     8281 tctaacaatc gaagggaacg gccaagtaag cttaacagat gaggaattag cgaaattgaa
     8341 tcgattgatc aaagaagcag gattcgaaga acagcttgaa gctgatcatg aaccgaagat
     8401 cgttgtcggg tacgtaaaaa catgcgtccc acatcctgat tctgaccatt tatcgattac
     8461 tgagacagaa gtagacaatg gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga
     8521 agctggacaa aaagttgtcg ttgcaaaacc aggagctatg atgccagatg gaatgatgat
     8581 ctggccagga actttgcgag gggtagatag ctatggaatg atctgttccg ctcgtgaatt
     8641 gcatttgcca aatgctccag caaaaaaagg aatcttagta ttgcctgagg atgccgtagt
     8701 cggagaagca tttccaaaat aaagaggttg tgacaagtct ttgtcacaac ctctttttta
     8761 ttaattttca ctattttgat tttgttttaa tgccgattga tcgatcgtca attcaacagt
     8821 agttgttttt tctttcgagc cttcatagta agtgattttg acagaatctc ccactttttt
     8881 cttatacaaa gcagattgaa gttctacacc agaagaaact tctttgtcat cgatcttagt
     8941 aatgacgtcg tattgtttca atccagcttt ttcagccggt gtagctgttt ggacatttgt
     9001 cacgatgaca ccgttagtta cagatgaagg aatcttcaag atttgttctt gttgctgagt
     9061 agaaacggca gataagtcga ccattgtgat acctagagct ggacgtgtca cttttccgtc
     9121 tttttccagt tgattgatga tattcactac atcgttactt ggaatagcaa atcccattcc
     9181 ttctacgctt acattagaag tagattcaga agtacttgcg atcttacttg agttgatacc
     9241 gataacttgt ccttcgatat taactaaagg accaccagaa tttccagggt tgattgctgc
     9301 atctgtttgg atcgcattga tattgactgt ttcgttcgat tcattcgtac ttgtcacttg
     9361 tcggttcaag gaagaaatga ttcctgaagt gacagaattt gcatattcag atcctagtgg
     9421 ggaaccaatc gcaatcgcag gctcaccaac cttcagagca cttgagtcac caaaagaggc
     9481 aactgtttcg actttgtctg aattgatctt caacaccgct aaatctgaat aagcatctgt
     9541 tccaactaat tcagctttta ccttcgtacc gtctttcatc aagacttcaa gaccttgctg
     9601 gccatctact acgtgattat tcgtgactac ataagcagta ttcccgtctt ttttgtagat
     9661 cacgccgctt ccttcactat aagcttccaa attactatca tctgagctgc tactttcttc
     9721 ttgctgcccg aatagtccgc caaaaccgct tgattggttt tgactttgta agttgataac
     9781 agaaacgaca gcgtcttgaa ctttatctac cgctttggtg atatcagaat ctacattgac
     9841 tttgacattt tccacaactg tttcaccggc agaattctga ttgccgcttg tagcagtaga
     9901 actattgttg ccagtgccca tcgctgcata aaaaatgcca gctgtcagta gtcctccaac
     9961 gatccctcca acaagcccca gacctaactt acgccagagg ctattatttt tacttttttt
    10021 catctttggt gtcacatttt ttctatccat gtgttcctcc tccaattaca ttcatttatc
    10081 tactgttagt atagtcttat ttgttgactt tagtctactt tgagactgtg aactttttat
    10141 gaaaaacttt aggaaaaatc attgttttca gatgaaaata tttgattttc tttttccaca
    10201 gatactgtgt aaaactacgc ggataggatt ggaaaaataa gggataaaac atatccacag
    10261 cctgtgtata aagtggataa attcttataa aacgtatgtt tgcctaatga atgtgatgaa
    10321 ataaggtaaa ataacactgt tttctgtgga taaacctgtg gatgttgtgc ataactgttg
    10381 gggaaatctt tttaacaaaa atagccaaaa taaacatata gacgaaaaaa ttgattataa
    10441 taaacaaagt gagcatgaag accaaaggag agaaaacatg aatatcaaaa ttatctcagt
    10501 aggaaaatta aaagaaaaat acctgatcca aggtatcaat gagtatgtca aacggctgaa
    10561 tgcctatgca aaaatcgaat tgatcgaagt tcctgacgaa aaagccccgg agaatttaag
    10621 cgaagcgcaa atgagacaag taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa
    10681 agaacaagaa tacgtctatg ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt
    10741 tgctaaacaa atcgaccaat taggtatcca aggaaaaagc caccttgttt ttgttatcgg
    10801 cggctcttta ggactcagcg aagccgtcat gaaaagaagc aatgctcaga tctcttttgg
    10861 aaaaatgacg tatccacatc aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc
    10921 cttccggatc aatgcggggg ctccatacca caaataatga tattttttaa gagaaaaaat
    10981 tatccctatt ttaaataata gcaataggta gtggtaggga gaaatttata aataactttt
    11041 ttatttgctt tggtagccaa gtaagttgca aaagaaaaat agatactgtt aaaaagataa
    11101 tgtagacata gaaacaaata cgtaaaattt tagttagaat aatggcaagg agaaaaaaca
    11161 tgatagatga aaaaaagtgt ttaaaaaatc tattagagga tatggatgat attgaagaaa
    11221 actatctaaa tggactggga aaacaaatat atttcagata cttaagctat ctaaaaatga
    11281 aataagacat agtaattttt tagctttttt gtttgctcca aatgaaaccc acaatttatc
    11341 tgatgagttt tttaaaatgt ttttgaaaag gtatatagat tctaatgatg ataccaaagc
    11401 tgctatcaat tattttgatg ctttacttaa tagctatgaa gatttaattg tttacagaga
    11461 aaataagaat attgatattt tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga
    11521 aaataaaatt ctgtcttcgg agtctggagg gcaacttaat aaataccaaa tatatataga
    11581 acgcaattat tctaactata aaaaaatttt tgttttttta actccagatg gaaatgaacc
    11641 cacaaatcct agttggggca taattactta caagaatatc gttgaagaga gtgtaaaata
    11701 ttttgtgtaa atagaaaaaa ggaagtccct tctgtagaat agagttacca caacacattc
    11761 acagaaaaga ggacttccat atgaacgatt ttactacaga aattatggaa atactaatca
    11821 ataaaggtga tttggatgat ttgtttcgtc gtcacctaga attggctatt aacgctttat
    11881 tacaagctga attaacggta tttcttgact atgaaaagta tgatagagct ggatttaatt
    11941 caggtaattc ccgtaatggg aattattcgc gctcttttaa aacagagtac ggtgaattga
    12001 atttagtaat acctagagat aggaatggag acttttctca acaaacttta cctgcttata
    12061 aaagaaccaa tgactcttta gaaaccacta ttattcagct agttcaaaaa ggaattacta
    12121 tgtccgaaat ctctgatcta atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta
    12181 tttcaaatat gagtaaaatt gtgtctgaag atgtttcggc atttaaagaa agaactttag
    12241 aagctaaata ctcagtcatt tttatggatg ctactcatat tcccttaaaa agacaaaccg
    12301 tatcaaaaga agctgtctat atagtaattg gtatcaggtt ggatggaact aaagaggttc
    12361 taggatttac tatagctcca actgaatctg cttatgtttg gaaagagatt cttcaagatc
    12421 taaaagaccg tggtttagaa gaggttttgt tagtcgtaac agatggttta agtggtattg
    12481 acgatagtat tcatagtgtt tatccaaatg ctcaatttca acaatgttgt gtccatatat
    12541 ctagaaatat cgctcataag gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
    12601 aattagttta ccaagcaagt tcaaaagaag aagcaaataa tcaaataagg tttatgatag
    12661 ataaatggaa aaagcagtat ccacgagtgg ttaaattgct catgaatcct gctatattaa
    12721 ccttttataa tttccctccg tcaatcagaa gaactattta ttcaactaat ttgattgaag
    12781 gttttaacaa acaattaaag aaatatacca aaagaaaaga gcaatttcct aatgaagaat
    12841 ctctggagag atttctagta tctcaattca acaactataa tcaaaagttt ctatgccgag
    12901 ttcataaagg ttttaaagaa atacatgata cattagaatc aatgttttaa cttgatcaac
    12961 agaatgggct tttccattta cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc
    13021 gtaaagttat aaaatattaa taaaaatatt ttattaatga aaagcttggt agcacggtta
    13081 ttcatatgct aatttatttc actttatagt aaggggagtt cttagtatat tgttcaaact
    13141 taaatttcat ataatttaat tatgtaaatt agctattcta agaacttcta cggataactg
    13201 cccgtctaaa atttaaataa gagcttagaa tcttcatata agtaaggtta atttttttgt
    13261 acaaaaaagt cggaatattc tgagttactg aaatgatata aaatcataaa ctaaatccct
    13321 tgattttatt gactttcaaa agtttatata tataagttaa tgattatggt aaaatatata
    13381 cataatcatt aactcagaat atttttaaga agggaaacaa tagtgagcta taatgtacaa
    13441 ccgttaagga caaatgaaga gatacaagat tttcagtttt ggctaagaag aacaagtaat
    13501 ccggaaagag atagtttttt atttttgttt ggaatcaata atgggttacg tatgtcagat
    13561 attataggat taaaagttgg agatatacga ggcaaaagta agcctattat tgttgaacat
    13621 aaaacaggta agcgaaaacc gatttttata gataatttga gagaagaaat tttattatat
    13681 actgaaggta aagaagaaaa tgattggtta tttccgagtc ggcaacaagg tcgacatata
    13741 acaagggatc gtgtctatca gatatatgca gatatagcag aaaaattagg acgagatgat
    13801 ataggaacac atacattaag aaaaactttt gggtatcact actacaagaa aactagagac
    13861 attgctacct taatgtttat ttttaatcat agctcgcaag caattacaaa aaggtacatt
    13921 ggtataactg aagatgaaat aggagcatca cttcgtggat ttaaactagg tgtataattt
    13981 aaaaattttt ataaacagta aagacaaaga ttaaaagcta tttttatcta gaacgatttt
    14041 atttcataaa gttcgcggaa taaaagctgg ggttactggt acttggtata ggttcgtgtt
    14101 atataaacta agtaattaaa taacattttt aggttggaaa aggatgaaat taaaaaaaga
    14161 gaatcaatac ggtctcaaaa acgcaaacgc tgcggaataa gaaaaatact gataactcaa
    14221 cgtttagcag actatctaat agggatagtc tgcttttttc gtttttgacc atacttttga
    14281 ccttctgcaa acttccttgc ttttcctctg tagaactcat gaaatctgca aaacgaattg
    14341 ccgtttcctc tgtctgcttt ttcgcgatat gggtgtatat atcatccata gtggttttaa
    14401 tatctgtatg tccaagtcga acatacactt ctttgacaga cgcaccagat tcaaagagca
    14461 gactacaatg ggtatgacga aaaccatgaa gtgtgatatg ttttaagtta ttctctttaa
    14521 tgatgatttt caaattgtga tttacatagt ctaaataaag gggcttattt tcttctgatg
    14581 aaaacaaaag ttgttcacca tcttttatag gtttgattct catttttaga tattatgccc
    14641 gcacgtattc atcacatttc tattgtgaat cgctttattc gaccgacatt tgacttttat
    14701 cataatatac tcggtttaaa attactaatg aaaacaatta atcaagacga tcataccatg
    14761 tatcacctgt ttttttcaga taatcaccac cgtgtgggaa ctgaactgac tttttttgaa
    14821 gtacaagaag ggaacaacca gttttttgga accaatacga ttgaacgaac gatactaaaa
    14881 gttccttccg aagcatcatt gcatttttgg gaaatatatt ttgaaacaca aggcgtttgc
    14941 cattatggaa tcgaatcatt ttctggacga cctatcttac gttttgaagg gccagatgca
    15001 acacgattag ctttagttcc cttacgcgaa tttgaagata ttgatgatta tttcccgtat
    15061 gtcacagacc agatccctac agagcatgcc attttaggaa ttgatgccat tcaactacgc
    15121 gtacaatatt ccgatgcgac tgaacgagag ttattgtcgg taggctggta gcacaaagaa
    15181 tacgtcagtt tcttttcggg tcatcaacga gtgacaatct tagaaaatca agacactcag
    15241 ttctatcaag aagttcagat tattcaagat cgtgagaatc cagttgccat tgaagggatt
    15301 ggtagcgtcc atcatgttgc gtttggcgta gaaaataaat ctgatttaca acgcatcgac
    15361 aaacaattac aagagcgcaa ttttataaat tcaggaataa aagatcgtga attttttgta
    15421 tcactttatt atcgtgaccc aaatcaatta ttgattgaaa ttgctacagg ggaaggaaac
    15481 ctagatgcaa aagcgtatga aaatcaaagt ccacgctttg aagaaattcc tttattttta
    15541 ccgcagtact taaatttcat tcgtgaacaa gtggagcaat aattagctaa tcaaatccat
    15601 gacgaagagt aagaggttga ctcataagtc aaaaaataaa gaaaaacgca tcaaatccat
    15661 tctaaagaat gttgatttga tgcggttcct atttttataa actatcattt tcgactaata
    15721 aaactactta tgagtcagcc tctctttttt tcttgatttt tatacctctt tctaggtaaa
    15781 ctaaacatct gccaaaaata ccgtgtctca ttgaaacatt ctcaactatt tcaaaataaa
    15841 ggggagtacc aaatgaacga gtatattgaa attatccacg caagagaaac aacttaaaaa
    15901 tgtcagtctc aaaattccca aaaataaaat tagtattttt accagcgtat ctggttcagg
    15961 aaaatcatcg attgtttttg atactattac ccaagaagct ggtcgtcaac tgaatgacac
    16021 ctatgatagt tttacccgtt tatttttacc taaatacagt cgtccagagg ttgatgagat
    16081 tgataatctt tctacggcga ttgtggtcaa tcaaaagaag attggtggca gtgcgcgctc
    16141 aacgttaggg acgattacta acatcaatgc gttatttcgt gtgttgtttt catgttttgc
    16201 aacacctagt ttaggatatg caaatgctta ttcatttaat gatcctagtg gaatgtgttc
    16261 tgtctgtcag ggaatcggta agacgattgc atttttaata ggattgcttt atttttcggc
    16321 tattgataat gctgattttg tattttctga tccatttacc gcagaagttt tgtctggaat
    16381 tgaggaacag gcaaagaatc atggttattt tgtattggtg cataatgtta attcagcaga
    16441 agatgttaaa actattcaga gaaattggcg ttttgagggg ttcattgttg ttggtgtacg
    16501 tgtaaaagaa tttttggaat taaataacgt ttttgatggt cctgttgttt atattgatac
    16561 gcatctttca aaagagacat taagagaact tgaacaacaa atgaatcgtt tatttgtaaa
    16621 tacaaatgat taccaagcta gtacattggc agtggaacat ttgattcatt atggtcatga
    16681 gaaaatagcg tttctttctt ttgactacat tgtaggtgaa ccaagtgtta ttcaagaacg
    16741 acatcaagct tatatcgata cattaaagaa atatcattta acctttaaat cgcattggat
    16801 atataacaac tctgaatttg agcgaattta tcaagaatta gctgaattta ctgcaattgt
    16861 tgttactgga gattatttag cggctaaatt agtaaaattt ttaaaaaata agaataaacg
    16921 agtgatggaa caattatcga ttattggatt tgatgatatt acttttgcag aattaatgga
    16981 tcctgcgttg acaacaatac gactaaatcc aggtaacaag ggggcgattg cttttcacca
    17041 actgattgga ctggccaaac aagaagaaat aaaaaatcaa gtactactat tagagggaga
    17101 attgatttct cgagactctg ttcacaaaat caaataaaaa agctctgtga aataagtata
    17161 gttaacaata aaatatccga acgatagaga gaatgaactg tgatctagca gttcctcgtc
    17221 gcaaattcac tagaattagc attatttact ctaaattgtt cggatatttt ttcttgtttt
    17281 taattatgat tacagtgcct atttttaacg aaatttgctt attttcaaaa tgtatgatta
    17341 tatgttagta aacactttca aatatgaaaa gaggagctat tatggtagga attagggatg
    17401 ttgcaaaacg ggcgggtgtg tccattgcaa cagtatcgcg cgtgctaaat gaggatccga
    17461 cactttcagt aaccgatgaa accaaacgaa agattgcaga atcggttaat ttttatcatt
    17521 atgtcaaaaa aagtcccgtt gcaaaaaatt taacaaatat tgcattaata acgacagttt
    17581 ctgaagtaga tgagttggaa gatccctatt ttcgaacgat tcgcagaggg attcaagtcg
    17641 aagcagaaca aagcaaagtc aatttgaaga aaattattcg tttgtcagaa acaccgttgg
    17701 agcaagacga tttgctaaaa tgtcaaggtg tgttgattat tgggcaggtg ctatcttctg
    17761 tgattgaaga agtatcagca attaattcga atgtcgtagt gattgatgac cctagtattg
    17821 aagctgaagt agatgcagtt tatactgatt taacaaaagc aacgaatcag catttagatc
    17881 gattgtataa aaaaggacac cgaaacattg cgtttatcgg aggaggacgt gttcgtttag
    17941 atagccaagg aaataaacac gtgtcagatg atgattatcg aaaaatagcc tatgagacgt
    18001 ggatgaaaca aaaaggatta gaaaaatata gtcaaattta tttagaaggc tggaccacac
    18061 ttgacggaat gcaggcagca gatttacttt tgaaaaaaca tgctaaagat aagttgccga
    18121 ctgctatcat ggtaggaaat gatcctattg ctgtgggggt ttatcgttca cttcaaaaaa
    18181 atggtctaca tattccagaa gatatttcga ttgtgagttt tgataatatt gaagttgctg
    18241 aatttttaac acctgcatta aatacggtga atgtgaatac agaagaaatt ggaaaactag
    18301 cagttcgcat ggttaatgaa cgaataaaag aagttagaaa tgtttcgatc aaagtgattg
    18361 tttctaataa tattattgtt agagaaagtg aaaaaaatat cgattaaaaa agtaaacatc
    18421 caaaaaaggg tgtttacttt tttgtttact aatattttta gtaaaaaatc tcgacaaaac
    18481 attaagtaaa cagttatggt gttgttaaca aaaagaaaac gcattcaaaa caggaggaat
    18541 taagatgaaa catttcaaaa aaatagcttt aacagggata gctgttttaa ctgggacatt
    18601 actcgcagca tgtggtgggg gagatagtaa ttctaaagac gggcaagtaa ctttaagcta
    18661 tgcgagttga gatactgacc aagctccagg actaagaaaa atgttagatg aatttgaaaa
    18721 acaaaatcct gatatcaaag tagaaatgga gacaacgcct tgggatcaat attgggttaa
    18781 attagaagct gcaacaacgg gaggtaatat gccagatgta gtaaccatgc actctagcga
    18841 atcctataaa tatatgtcac agaatgcttt gatgggactt gatgatgtta tcaaagaaaa
    18901 tgatattgac atgggaaact ttactgaagg aattgctgat ttctatacgc ttgaagacga
    18961 actatacgca atccctaaag atgcttctgt agtggggcta tggtacaaca aagaattatt
    19021 tgataatgca ggtgtcgatt atccagatga aacatggaca tgggaaacac tcagacaagc
    19081 tgcaattgat ttaacagatg aatcaaaagg catctatggt tttgcatcac atattggaac
    19141 agagactggc tactggccat ttatttatca aaatggggga gaagtatttg ccgatgacaa
    19201 tacaaaatca ggtttgaata ccccagaagc acaagaagct ttagcttttt atcgagatat
    19261 tgcacttgtt gatcatgctt caccaacaat tacagaatta caagatcctg ataagttaag
    19321 tcgttttcaa tcaggtagat tagcaatgat gattgaagga aactggtttg caccgggatt
    19381 tttgaaaaat gactatctag ttgaaaatgt tgcttctgcc gtgttaccta agggaaaagt
    19441 taatgcaaca attacaaatg gacttggttg ggctgccagt gcgagtacaa aatacccaga
    19501 agaagtgaaa aaactaatgg catttttagc tagtgaggac gccaataaaa tccaagcgga
    19561 aacgggggca tcaatcccag cactaaaagg ctttgaagac gagtgggctg cttcttcaga
    19621 tacaattgat ttgagtgctt ttggaaaaat gttggagtat ggagtccctc gtccatacaa
    19681 taaagatgga ttacgagctg aacaagcaga agttcaaatt attaaccgta ttctgtctgg
    19741 tgaagtaact gttgaagatg gaaccgctca aattgaaaaa gaagtcaatg cgatattgag
    19801 cggaaattaa attagttaat tatacaatat acaggcgaaa ggattctatt tcgtctgtat
    19861 attaatggat gaataccaaa aagggggata ttatgaaaaa tcgaattttt ggaaaatatc
    19921 gtaaagatca agtgatatgg ggctatattt ttatttttcc aacggcatta ggattaattt
    19981 tattaaatat atggccagca attcaaacag caattttaag ttttcaaaaa acggtagggt
    20041 tcggtgatac tgaatgggtc ggattaagaa attaccaaaa aatgtttgca gacggtgaag
    20101 tttttcagtc tttattaaac acattaattt atgcagtggt ttcgatacct agtattgttg
    20161 tattatcctt gttggtggca gttttaatga atagaaaagt taaaggattg tcgatttatc
    20221 ggacaattta ttttctacca gtcgttgctg caccatccgc agtcgcaatg atttggcgct
    20281 ggatgttcaa taatgattat ggactgatta ataacatgtt ggctaaaata ggtctcgatg
    20341 gaccagcatg gttaacggat ccatcgattg cgatttattc tattatcatt gtaggagttt
    20401 ggagtgctat tggttataac atggtgttat tactagccgg cttataagaa attcctaaag
    20461 attattatga agcagcggaa attgatggtg ctagtccaat ccagcagttt ttcaacatta
    20521 cgttaccatt agttacacca acattatttt ttgtagttgt aacaacagtt atcaatgctt
    20581 tccaagtatt tgatgttatt tttatgatga ttactcctaa tagtaccgcc atgtttaaat
    20641 cacaatcatt agcctattta ttctataaac attcatttat tttaaatgac aagggatatg
    20701 gttcggcaat cgttatgttc cttttagtga ttatattaat tattactgca attcaaatga
    20761 agttgcagaa aaagtgggta aactattaag gaggctatca aattatgaaa aataaatttt
    20821 tttcaaatgc gccaatccat atcatgctta ttttaggtgg tattgcaatg gcattacctt
    20881 ttatttggat gattttaact tcagggaaaa cgttggttga atctacccaa attccaccta
    20941 caattttccc tgagaaattt cagctaagta actatcaaca agtacttgat ttattaccat
    21001 tcatggcttt ttatatcaat acatttttaa tggttgttgg acgagtgatt ggctcggttt
    21061 tattttcagc aatggctgca tatgcttgtg cgcgtctaag ttttccaggg aaaaatatcc
    21121 ttttcagctt agtacttatt caaatgatga ttcccggttc catttttatt actccacagt
    21181 atttattagc acaaaaattg ggtattttaa atacaattcc agcattaata ttaccagggg
    21241 tagttagtgc gtttggtacg tttttattaa gacaattttt cttaggttta cctgatgaat
    21301 tggaggaagc ggcctattta gaaggtgcca atatttggac gatctttttc aaaattatgt
    21361 tgccgcttgc taaatcagga ttggttgcac ttggaatatt cacagcgttg tttgcattta
    21421 aagatttaat gtggccattg attgtcaata cttcaattga taagatgcct ttagctccgg
    21481 gattagcaaa cttacaagga caatatgcca cgaacttccc acaattaatg gcaggttcgt
    21541 tattagcaat ttggccaata gttgtgatat tcatcgtttt ccaaagaaaa ttcattgaag
    21601 gaattgctac aacaggtagt aaattataaa actattaatt agtgattgga gagtaagtac
    21661 atgacaaaaa ttacatttat tggtgcagga agtactattt ttgcaaaaaa cgtattaggc
    21721 gatgcgatgt taactccaag tctacaagat gcacatattg cattgtacga tattgatgaa
    21781 aaacgactaa aagaatcaaa tttaatgctt aaaaatatta ataaaaattc gaatgaaaat
    21841 cgtgctgata ttgtttctta ttatgatcgc aaagaagcgt taagagaggc tgattttgtt
    21901 atcaatgcta ttcaagtggg tggttataaa ccttcaacag tgattgattt tgatattcct
    21961 aaaaaatatg ggttgcgaca aactattggt gacaccatgg ggattggtgg gatttttaga
    22021 accctacgga cactaccagt aatgttagac tttgctaaag aaatggaaga agtttgtcca
    22081 aatgcttggt tattgaacta tacaaatccg atggcgatgt taaccatggg tgttttaaaa
    22141 gcgacgagta ttaagactgt tggtttatgt catagcgttc aagtatgcgt accagaattg
    22201 tttgagcatt taggaatagc cgatcaatat gacttaaatg actttcaatg gaaaattgca
    22261 ggcattaatc atatggcatt cttgttggaa attacaaaaa atggggaaga tttctatcca
    22321 acaattcgtc agttagctgc agaaaaacca aatccacatc cagattctgt acgttttgaa
    22381 ttgttgaaac gttttggtta ctatattaca gaatcaagtg aacacaatgc agaataccat
    22441 ccatacttta ttaaatcaaa atacccagaa ttgattgacg aattagggat tccgttggat
    22501 gaatacttac gtcgttgtga acaacaaatt aatgattggg aaagcatgcg agaggacatt
    22561 gtaaataatg gggagttaac acatacgaga agccgtgaat atgcttcata tatcatggat
    22621 tctattacga caggaactcc gtcagtgatt gcaggaaatg tcttaaataa aggcttaatc
    22681 acaaacttac cgacagatgt ttgtgtggaa gtaccttgtt tagtcgataa aaatggcgtg
    22741 caaccaacct atgtcggtga attgccaaca caattagctg ctttaaatcg gacgaatatc
    22801 aatgttcaag aactgacagt agaagccgct ttaacactag ataaatcaaa aatttatcaa
    22861 gcagcgtatt tagatccaca tctatcatct gaattgtctt taacagatat caaaaattta
    22921 gtggatgatt taatcgaagc acatggggac tatttacctg aatataatta aaaagaagct
    22981 gaaagagtaa ggaattttca attccttact cttttcttag ttaaatttag taaaaatatt
    23041 gacaaaacac ttagtaaaca gttacattag tgttaacaaa aagaaaacgc attcaaattg
    23101 tataaggagg agcataaaat gaagtggtct aaaaaaatta ttttagcttg tatagcacta
    23161 ggagcaacga tctctttgac tgcttgtgga aaaaatcagg aagatgcaag cggtaaggta
    23221 gaaatagagt tttttaatca aaaaaaagaa atgactcaaa cgattcagga gattgctaaa
    23281 gattttgaag caaaaaaccc tgatattcat gtaaaagttg tggatgtacc gaatgctggt
    23341 gaagtaatta aaacacgtat gcttgcaggc gatgtaccag atgtcattaa tctttatcct
    23401 caaagtatag aattgaaaga atgggcgaaa gctggttatt tagaggactt aaccgaagaa
    23461 ccgtatttag agaatatcaa aaatggatat gcacaacgtt ttgctattga ggatcgtgta
    23521 tatagcatac cattaaccgc taatgtctat ggtttttact ataacaaaac tgcttttgag
    23581 gaaatgggga ttcaagcacc agaaacttgg gcagaatttg aaaaaatagt agctgagatt
    23641 aaagatcaaa acaaagtacc gtttgcgatt gcaggagcag aaggctggac tttaaatggt
    23701 tatcaccaac tagctatagc gactgctgct ggtggtgaag aggaagcgaa caatgtttgg
    23761 cgtttctcag aagttaatgg aattaatgct gaaagcaaag aaatgcaaaa agattttaat
    23821 cgattagatt tgttaagaga accaaaagct ttgcaaaaca actggcaagg tgccggttat
    23881 aacgatacag tagttacttt tacaaaaggt gatgcgttga ttatgccgaa tggttcatgg
    23941 gcaatgccga tgattcattc acaaaatcca gattttgaag taggaacttt tccttttcca
    24001 gcagatgaag caggtcaaag tctaacgatt ggcgcaggag acctagcctt atcagtttct
    24061 gcaacttctc aacataaaga agaagccaaa cgttttgtag aatatatgac aacacctgaa
    24121 gcgatgcaga aatattatga tgtagatggt gcaccgtgtg ctgttgaggg cgttatcgag
    24181 aatactcagg attcaccatt aagtggttta actgaacttg cttttactga tcgtcattta
    24241 gtttggttag ccaaagattg gaatagtgaa aatgattttt ataccttaac aacaaattat
    24301 ttacacaatg gcaatcaaaa agccatgatt aacgcattaa atgctttctt taatccaatg
    24361 aaagctgacg gaaggtagga aaataaaatg aaaataaaag aaattttaaa tcgttactgg
    24421 gcaatcattt ttgtagcaat tccaattatt ttgcagatga tttttttcta ctttccaatg
    24481 gttcaaggtg ctttttacag tttaactaat tggactggtc tgacttataa ttttgatttt
    24541 gtgggtttga acaattataa gattcttttt tctgatccga agtttatcaa atcgattggc
    24601 ttcactttaa ttgtgacgct ttgcatgatt gctggacaaa ttggttttgg tattttgatt
    24661 gcgcgggcgt tgaacgcaaa tattaaaggg aaaacattct ttagagcatg gttctttttt
    24721 ccagcggtct tatcgggcgt ggttgtttct ttagtattta aacaaatttt taactatggt
    24781 ttaccaaaaa ttggtagtat gttaggcatt gaatttttac aaaccagtct tttaggaaca
    24841 acatggggag cgatttttgc agtaattttt gttttacttt ggcaaggaat tgccatgcca
    24901 gtcatcattt tcttagcggg ttttcaaagt attccacaag aaattattga agctgctaga
    24961 atggatgggg cgaatgataa gcaaattttt tggaatattg aaatcaccta cttattacca
    25021 agtatttcaa tggtatttat tatggcatta aaaggtggct taacagcatt tgatcaaatt
    25081 tttgctttaa ctggtggtgg accaagcgat gccacaacat ctttggggtt acttgtttat
    25141 aattatgctt ttaaaaacaa tcagtttggt tatgcgaatg ctattgcttt agtcttattt
    25201 atcttaattg ggattgtttc actggcacaa ttaaaattat cgaaaaaatt tgaaatctag
    25261 gaggtttccc aatgaaaaca tcaaaagaaa aaaatatttc taaatacttg cttttattat
    25321 tagggggctt catgatttta gttcccttag tttcaacgct attcggttca tttaaaacaa
    25381 cacccgatat tatgaataat ttctttcgtt ttcctaatcc agcgactttg gaaaatttcc
    25441 gaagattgtt agcggatgga attggtcatt acttttggaa ttcggcaatc atcacaatca
    25501 ttgcagtagt tgctgtgaca gcaattattc ctatggctgc ttattcacta gcaagaaaaa
    25561 tgactcgtgg gaaagcatac ggattgatgt atttattttt aattttagga atttttgttc
    25621 ctttccaagt aattatgatt ccgattaccg ttatgatgag taaaattggt ttagcaaata
    25681 tgtggggatt aatcttactc tatctcactt atgccgtacc acagacacta tttttatatg
    25741 ttggttttat taaactaaat gtgcctatca gtctggatga ggctgctgaa attgatgggg
    25801 ctagtcactt tacaacttat ttcaaaattg ttttcccaat gttaaaacca atgcatgcga
    25861 cgactattat tcttaatgct ctatggtttt ggaatgattt catgctacca ttattaattt
    25921 taaatcgtga ttctaatatg tggacactac cattgtttca atataattat acgggacaat
    25981 attttaacga ctatggtcca agttttgctt cctatacagt aggaattgtc acgattacac
    26041 tagtttatct tgctttccaa agaagtatta tagctggaat gagcaatgga gcagtgaaat
    26101 aaccaataaa ttattctcat tataaaggag ttcataaact tatgattata tttgacaatc
    26161 acacacaaac atttcattta caaaataaaa aaattagcta tctattagga atcgaagaaa
    26221 ataattattt aacgcatctt tattttggaa aaaagattac gcactattct ggtgggtatc
    26281 gctacccccg aacagatcgt tccttctcac ccaacaatgc aaatgcgcaa gatcgtttat
    26341 tatctttaga cacattacca ttagaatttc caggatatgg acatggtgat tttagagaac
    26401 cggcgcataa tattaaatta ggaaacggta gtcgtgtaaa tgattttcgt tatgatagct
    26461 acgaaattca aaaaggaaag cccagcttag cgggattgcc agccttatat acggaagatg
    26521 atgatgaagc tgaaacgctg attattcaat tgaaagacgc gattagtgga ttgactttac
    26581 agttgttcta cacaatttat caaaatcaca gtgtaattat tcgttctacg caattagtaa
    26641 ataatagtga tgaaacagtg gagattaacc gcttagctag tcaatcaatt gattttccaa
    26701 atcgtccact cgagttaatt catttaaatg gtacctgggc aagagaacgt caaatgacta
    26761 gagaaaaaat tcatacaggg ataaaagtat tagatagtaa acgcggatca agcagccatc
    26821 aacaaaatcc gtttgttgct ttagtagatg ccaaaacaga tgaatttcaa ggagaggcat
    26881 atggatttaa tttagtttat agcggtaatc acgaaacggt gattcaaaaa gatccatttg
    26941 accaaacacg ggtcattatg ggaattaatt catttaattt tggttggcaa ctaaaaactg
    27001 gcgactcatt tcaaacgcca gaagtcgtga tggtttatag taatgaaggt ctgaatacgt
    27061 tatcacagac gtatcatgaa ttgtataatc accgtttaat tcgtggaaac tatcgtttaa
    27121 aagaacgtcc cacgttaatc aacaactggg aagcaacgta ttttgatttt aacgaagaaa
    27181 aaatcatgaa tattgtcgat gatgcgaaga acttgggcat tgaaatgttt gttttagatg
    27241 atggttggtt tggcggtcgt gaaaatgact tcacttcatt aggtgattgg gttgaaacag
    27301 aaggcaaact tgcttgtggt ttggaaaatt tagccaagca agtgcatgat aacgggatga
    27361 aatttgggat ttggtttgaa ccggaaatga tttctgaaga tagtgaacta tttcgagcac
    27421 atccagattg ggcattgggt attccaggtc gtgaccgctc aattagtcga aatcaatatg
    27481 ttcttgattt ttcaagacaa gatgttcgtg ataatatcta tcaacaaatg acggcgattt
    27541 ttgatcgtgt accgattgat tatgtgaagt gggatatgaa tcgaaatatg acagaagtct
    27601 attctgtctt gttggactca acgatgcaag gtgaagtggc tcatcgttat atgttagggt
    27661 tatatgaatt tttagaaaag ttaacaacga attatccaac tattttattt gaaagttgtt
    27721 cgggtggagg cggtcgtttt gatgcaggtt tcttgtatta catgccacaa acttggacga
    27781 gcgacaatac cgatgccgtg gctcgtctga aaattcaata tggaaccagc ttagtgtatc
    27841 caatttcaag tatgggtgcg catgtttcag cagtcccaaa tcatcaaaca ggaagagaaa
    27901 cgagtttaac tattcgtggc aatgctgcga tggctggtgt ttttggttat gagctagatc
    27961 taacgcaatt aacttctgaa gaaaaagcag aaattaaaga acaagttgcc ttttataaaa
    28021 agcatcgcca acttttgcaa tatggaactt tttatcgctt ggaaagtccg tttgaaggca
    28081 atgatactgc atggatgttt gtctcaaaag accaaacaga agccatggtc ttttatttcc
    28141 gagtattagc agaagctgcg catccattag tcacattgaa attagcagga ttagcagaag
    28201 aagcaactta tcaaatggaa gatgtaacat ttgctggcga tgaattgatg aatctcggtt
    28261 tttatattga ctcagaacta catggtgatt atgcgacaca acgcttttat ttgaaacaga
    28321 tatagtagct tgaatttaca atataagtgc aacaccaaaa gagactcatt ccaaacgaag
    28381 aaaatatgtt ttatttacac gcatttacta aaaagcagcc tgatttgaat tgggaaaatg
    28441 aagaagtacg tgaagaaatc tatgcaatga ttaactattg gttagataaa ggactaggcg
    28501 gatttagaat cgatgcgata ttaaacatca aaaaaaaact agagtatggc atctttgaat
    28561 ctgatggaga agatgggtta gccttcattg ggccctggat tcttaatcag tcgggcattg
    28621 aagtgtggtt aaaagaaatg agagagcgga catttaaacc acataatagt atgacggtag
    28681 cagaagccga tgttcctaat gaacgtttag atgaatatat tggtgaagat ggctattatt
    28741 cgatggtttt tgattttagt tatacggata ttgatgtacc tgaaactggt gaatggttta
    28801 aagatagcca atggacatgg acggatatgc gtaccaatat ttttacgaat caattagtta
    28861 cgcaagataa aggttggggc gccttatatt tagaaaacca cgatcaacca cgttcaatta
    28921 ataaatatat tccagaagaa tggatcaatg attatagtaa aaaaatgttg gcgacgcttt
    28981 ttatgttact aagaggtacc ccttttattt atcaaggaca agaactagga atgaccaaca
    29041 tagaaatggc atcattagaa gattttgatg atgtagctac gcattctcag tataaacggg
    29101 cattagaata tggattggca ccagagcaag cattgaaagc tgtcaataaa cgaagtcggg
    29161 ataattctcg cacacctatg caatggaata cgcaaaaaaa tgccagattc tcaacttctg
    29221 ataatgtatg gttaaaagta aatcctaatt atccagaact caatgcagaa gctcagttaa
    29281 ccaatcaaca gtcggtattc aattattatc atcaattaat taaattaaga aaaaatagtg
    29341 tatacaaaga cgtgctaatc tacggacaat ttctaccagt gaaagataaa aatgaacata
    29401 ttttgcttta tgaacgccaa ttagaagata aaatcgtatt agttatctta aacgttacag
    29461 caacagaaca agaatattat gtagatgaac agtaccaaca agtattaatc aataattatg
    29521 aagatgtttt attagatcaa agtcgaaaat tgcgtttacg accgtttgaa agtattgtat
    29581 tagctaatta tggaggacaa ttatgaaaat taaaaacgaa gcaatgctca tcacctatgc
    29641 agatagttta ggaaaaaaca tgggcgaatt aaaacaagtc ttagacaaac atttacaagg
    29701 tgttattggt ggtgtacatc tgttgccttt ttttccatcg acaggtgatc gaggctttgc
    29761 gccgagtgat tatacgacag ttgatccagc tcttggaaca tgggaagatg tggaagcatt
    29821 aggtgaagat tattatttga tgtttgattt tatgattaat cacatttctc gtgaatcaaa
    29881 atttttccaa gatttcaaaa aaaaccatga gcagtcacca tacaaagaaa tgtttatccg
    29941 tattcatgaa tttttcccag aaaatcgacc aacacaagaa gatattgatt taatctacaa
    30001 gcgtaaagac aaagcgccgt ttcaaacggt agaatttgcg gatggtacga cagaagaggt
    30061 ttggaatacc tttggggaag aacaaattga tttagatgtg accaaagaag ttgtgaaaga
    30121 atttattcga gaaaccatca aagatatggc gaaccatggc tgttctttaa ttcgattaga
    30181 tgcgtttgct tatgcgatta aaaaattaga taccaatgat ttcttcgtgg aaccagatat
    30241 ttgggaactt ttagacgaag tgcgtcaaga agcagcgaaa tatgacgtcg aattattacc
    30301 agaaatccat gagcattatt ccattcaaat gaaaattgcc aatcatgatt attatatcta
    30361 tgacttcgcc ttgccaatgg tcactctgta ttcactttat agtggaaaat cagaacgttt
    30421 agccaattgg ttgaaaatga gtccgatgaa gcagtttaca acattggata cgcatgatgg
    30481 aattggtgtt gtggacgctc gtgatttatt aacagatgaa gaattggatt acacctctga
    30541 ggaattgtat aaagtcgggg cgaatgtgaa gaaggtctat tcaagtgcca attacaataa
    30601 tttagatatt tatcaaatta acagcaccta ttatagtgca ttaggcgata atgaccgtag
    30661 ctatctgtta gcacgtgcga ttcaagtatt tgcgccaggg attccacaaa tttattacgt
    30721 tgggttatta gcaggaaaaa atgatattga actgttggaa gcaacgaaag aagggcgtaa
    30781 tattaatcgt cattactatg atttagccga aattgaagaa gaagtgcaac ggccagtgat
    30841 tcaacaatta tttgcacttt taaaatttag aaatgaatct gcggcgtttg atttggatgg
    30901 aacgattgaa gtagcaacgc catcagaatc agaaatagtg attacacgta aaaataaaaa
    30961 cggtagtgat agtgcaacat taacggctaa cttggatacg aaagaattta tcattgagtc
    31021 taaagatata aaaattttca ctagtgttgc attaaaattt taacctattc catagaacaa
    31081 aaaatccttt atactagcac ttggatgact tatccaagac caataaaaag gactcataca
    31141 tggataagta taaaacaaat acagcattta ataaatggtt ttctgccata aaattaggaa
    31201 aactttctgt caactcccaa aaatgtattt ttgattacaa ttcgtatcgt aaaaaactgt
    31261 cttttgaagc tgtgctcaag ctctttctct acgcagttaa tgaagaaaaa gaaagtttgc
    31321 gtgatttgag tacagctctt atcgaccgat cgctccaact agaaaccgat gtgtcatcga
    31381 ttagtcatac ccaattgtct cgagcattta atgcacttga acctaaagtg ttagaagaaa
    31441 tttttcaaca actgttagaa aaagttcaac atcaaatcaa accaaccaag cgaaatcatc
    31501 tgtatctgat tgattccagt acgttttcgc ttagtttaaa acgtcataag tgggcgactt
    31561 tccgtcaaac aaaatcaggt gtcaagttac acttaaacta ttgttatatg gacgatacta
    31621 cgatgtatcc aactgatttt accttaacaa atgcccatga acatgatctc aatcaactac
    31681 cagtattggt gaatcaacca gaggcaactt acgtgtttga tcgtgggtat taggactttg
    31741 aaaagatgga tcagatgcat tgggatggct atttcttcgt cactcggatt aaaaaaaaca
    31801 ccaaagttca cgtgatagat actctggaaa catcgccaga aacagaaatt ctacgagacg
    31861 agctggttcg tttaggatct aaaacctatc taaccgccaa tttccgacta gtcactgttc
    31921 aagataagaa cggacgtgtg tttcaattta tcaccaatcg aatggacgtc tcttcaaaag
    31981 aaatctctga tatgtatcac gctcgttggc aaatcgaatt gtttttcaag cacatcaaac
    32041 aacacatgac cattaaaacc ttcttttctc aaagtgaaaa aggcgtgcag aatcaattga
    32101 ttttaacgat gatttccgct ctcctcacgt ttctcatcaa attggagacg aaaacggaaa
    32161 aatcagtgtt ccaaatcaaa cgattctttc gttatttact gtttcagccc tttgaatgtt
    32221 gtctagaaaa attaattccc acatgagaaa aaagaaattt ggctcgttac ttttggtttt
    32281 cagttgaata acagataaaa aattttctgg aaaaagtcat cctctgcgtc agcacagttt
    32341 tttttgactt attttcagag gaaatagttt tcagaatatt ctaaaatgtt ttatgcaaca
    32401 ctagtgatat tttttagaat tagatttcta atgtgcattt tttgagtatt aaggagcaag
    32461 tatacaaaga tttataaagc tgattaaatt tgaataaaaa tatgcaggaa cgctactatt
    32521 tttagtagtg ttctttttta tatttgaata aaaaatgtgg tggttaaatg gtggttataa
    32581 caaaaaatga attaatgaaa ttaggttatg ggaagtatca gtctgagaga atcatacgac
    32641 aggcaaaagc aattatggtt tcaaaaagct atacctatta cgaaaataga agattaggac
    32701 tagtacctgt tgaatctgta gaagaagttt taggtatgaa gatagaggta gaggaggatt
    32761 ttcagaatca tggttaattt taaaaaagat aaaaatggga aatatcattt tactgctagt
    32821 ttgggattcg acaaacgaac agggaaaaga gtacaaaaaa acgtagaggg gttaaaacag
    32881 ttaaagaaga aaaaaagcgt ataccgaact tgtttctaat tatggaaaag aagcagaaac
    32941 ttctcaaagt actatgttgt ttgaggagtt tttttataaa ttattcctac cttattatca
    33001 agggaaagta g
//
LOCUS       DASJPC010000026        29548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJPC010000026 DASJPC010000000
VERSION     DASJPC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.026"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(306..1154)
                     /locus_tag="U4A13_001142"
     CDS             complement(306..1154)
                     /locus_tag="U4A13_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1779452.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1378..3318)
                     /gene="thrS"
                     /locus_tag="U4A13_001143"
     CDS             complement(1378..3318)
                     /gene="thrS"
                     /locus_tag="U4A13_001143"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN1779453.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3655..3834)
                     /locus_tag="U4A13_001144"
     CDS             complement(3655..3834)
                     /locus_tag="U4A13_001144"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN1779454.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3881..5704)
                     /gene="cadA"
                     /locus_tag="U4A13_001145"
     CDS             complement(3881..5704)
                     /gene="cadA"
                     /locus_tag="U4A13_001145"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1779455.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5982..8261
                     /locus_tag="U4A13_001146"
     CDS             5982..8261
                     /locus_tag="U4A13_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1779456.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8354..9001)
                     /locus_tag="U4A13_001147"
     CDS             complement(8354..9001)
                     /locus_tag="U4A13_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779457.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9180..9899)
                     /locus_tag="U4A13_001148"
     CDS             complement(9180..9899)
                     /locus_tag="U4A13_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1779458.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(10030..11181)
                     /locus_tag="U4A13_001149"
     CDS             complement(10030..11181)
                     /locus_tag="U4A13_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN1779459.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11398..12138)
                     /locus_tag="U4A13_001150"
     CDS             complement(11398..12138)
                     /locus_tag="U4A13_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1779460.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12142..12465)
                     /gene="rsfS"
                     /locus_tag="U4A13_001151"
     CDS             complement(12142..12465)
                     /gene="rsfS"
                     /locus_tag="U4A13_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN1779461.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12522..13115)
                     /gene="yqeK"
                     /locus_tag="U4A13_001152"
     CDS             complement(12522..13115)
                     /gene="yqeK"
                     /locus_tag="U4A13_001152"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN1779462.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13099..13749)
                     /locus_tag="U4A13_001153"
     CDS             complement(13099..13749)
                     /locus_tag="U4A13_001153"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN1779463.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13759..14070)
                     /gene="yhbY"
                     /locus_tag="U4A13_001154"
     CDS             complement(13759..14070)
                     /gene="yhbY"
                     /locus_tag="U4A13_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN1779464.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14086..15195)
                     /gene="yqeH"
                     /locus_tag="U4A13_001155"
     CDS             complement(14086..15195)
                     /gene="yqeH"
                     /locus_tag="U4A13_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN1779465.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15192..15719)
                     /locus_tag="U4A13_001156"
     CDS             complement(15192..15719)
                     /locus_tag="U4A13_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN1779466.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15899..16684)
                     /locus_tag="U4A13_001157"
     CDS             complement(15899..16684)
                     /locus_tag="U4A13_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN1779467.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16677..17546)
                     /locus_tag="U4A13_001158"
     CDS             complement(16677..17546)
                     /locus_tag="U4A13_001158"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN1779468.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17547..18926)
                     /locus_tag="U4A13_001159"
     CDS             complement(17547..18926)
                     /locus_tag="U4A13_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN1779469.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18934..19353)
                     /gene="fabZ"
                     /locus_tag="U4A13_001160"
     CDS             complement(18934..19353)
                     /gene="fabZ"
                     /locus_tag="U4A13_001160"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1779470.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19378..19854)
                     /gene="accB"
                     /locus_tag="U4A13_001161"
     CDS             complement(19378..19854)
                     /gene="accB"
                     /locus_tag="U4A13_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN1779471.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19857..21092)
                     /gene="fabF"
                     /locus_tag="U4A13_001162"
     CDS             complement(19857..21092)
                     /gene="fabF"
                     /locus_tag="U4A13_001162"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN1779472.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21111..21848)
                     /gene="fabG"
                     /locus_tag="U4A13_001163"
     CDS             complement(21111..21848)
                     /gene="fabG"
                     /locus_tag="U4A13_001163"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN1779473.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21860..22777)
                     /gene="fabD"
                     /locus_tag="U4A13_001164"
     CDS             complement(21860..22777)
                     /gene="fabD"
                     /locus_tag="U4A13_001164"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN1779474.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22884..23108)
                     /locus_tag="U4A13_001165"
     CDS             complement(22884..23108)
                     /locus_tag="U4A13_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1779475.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23161..24123)
                     /locus_tag="U4A13_001166"
     CDS             complement(23161..24123)
                     /locus_tag="U4A13_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN1779476.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24133..24573)
                     /locus_tag="U4A13_001167"
     CDS             complement(24133..24573)
                     /locus_tag="U4A13_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1779477.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24879..25844
                     /locus_tag="U4A13_001168"
     CDS             24879..25844
                     /locus_tag="U4A13_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN1779478.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25901..26131)
                     /locus_tag="U4A13_001169"
     CDS             complement(25901..26131)
                     /locus_tag="U4A13_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN1779479.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26217..27104)
                     /locus_tag="U4A13_001170"
     CDS             complement(26217..27104)
                     /locus_tag="U4A13_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN1779480.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27193..28416)
                     /locus_tag="U4A13_001171"
     CDS             complement(27193..28416)
                     /locus_tag="U4A13_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1779481.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28433..29479)
                     /locus_tag="U4A13_001172"
     CDS             complement(28433..29479)
                     /locus_tag="U4A13_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1779482.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8206 a         6462 c         5075 g         9805 t
ORIGIN      
        1 atctttcatc atttcctatc cttccgccta tttacattca tgcttcattg tacccaaaaa
       61 cttcttaata aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa tttcaagata
      121 aaatcaactt tttgtttcac agcaatcact taaagttaca tatccaacta tttttttcat
      181 taaaaaataa acttattttt gaaaattatg ttttcttaag tcgtgaaaca ctttgatgga
      241 tcatttattt gtggaaaact acgttttgtt attctaaaaa acttatataa aaaatagtaa
      301 ggtttttatt tcgaatccat aaaatattct tggattgctt ctcgtgtttt tttcactttt
      361 tcgctaagct tttcgttttt agccatatag aaataaaaaa tgatatcgat cgtagcaaat
      421 tgggccaaaa gtgaattagt tgccgcactt cgattcgtga tttcttttgg tctagatgtt
      481 tgtacgagta catcggctat cttgctaagc gggttatttc caaattgcgt caacgcaatg
      541 atttcgatat tcatgctttt cgccagctcg gctaatgcga ctacatctgc acttctacct
      601 gagtttgaaa caagccaaag cactttcttt ttctcattgc tgacaagttg cgggagcaag
      661 acgtggatat ctttttcaaa aatgattggc ttaccgacac gagaccattt ttgcagaata
      721 tcttctacta ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc tgccgattcc
      781 aaacactcta cgactttttg gatcgtttct tcttctagaa tatctactgt ttcttttatc
      841 gctacttgtg cattgttacc aagtttgttt ttgatagagt aaaaggactc atttgcttct
      901 atatcactaa aaccttcatc gattccagtc tgtagttctg ttgaaatcgt gattttgaag
      961 tccgtcagac tttgaaaacc gatttttttg gaaaaacgta caactgtcgg cgcactcaac
     1021 ccggaagctt ctgctaattc attagctgtc attgtaggga ccttctcaat attaccgata
     1081 atgtagttgg ccaacttttt ttctgctgaa gaataatctt ccatcatact ttcaatacga
     1141 ctaattacgc tcataagttc ctccaccctt ttaatgatag tctatgtaag tataccatat
     1201 cagaccctta ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc gcccaacaga
     1261 atatcttatc ccatcaatcc cacagattct attgagcatc tgtgtctttg aggaatacct
     1321 gttatctatt gttcggattg ctttctatct actacttttt tacaggaaat ttacctatta
     1381 gttttctctg ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc
     1441 ttctacagct gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc
     1501 cttgtctcca acaactaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata
     1561 gcccattttt tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc
     1621 tttgatttca taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg
     1681 tataggtgcc aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa
     1741 acgttccatt gtagaaacaa ttccgcggtg gataactact ggacgatgcg tattttcccc
     1801 gtcttctcca acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt
     1861 tgataatgtt tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta
     1921 aaaggctgcc tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt
     1981 cagcattgct tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt
     2041 tggatcgcga tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc
     2101 caccatcaat tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt
     2161 atgaccatca ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt
     2221 ttcatagcga tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg
     2281 gatcgtgttt ttgtagacca tcatgtgatg agggcagttc attgggcgaa gaacaagcat
     2341 ttcgccatct cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc
     2401 agatgttttg tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag
     2461 actaacttct ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg
     2521 aagccagaat ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt
     2581 tcctagttta cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa
     2641 agctttcttg tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt
     2701 tccacgccaa taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga
     2761 tggaacatgt ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac
     2821 ttcttcttct ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc
     2881 taatgcttcc tgttttgaaa gaacacggcg ttcgatcggc agattttctt tgacgatttt
     2941 catcatttct gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc
     3001 tgtatcgtag taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata
     3061 gaggcgacgc atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc
     3121 atcttcatgg tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa
     3181 atcaacgagt tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat
     3241 gctttcagca attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg
     3301 aaaagtaatt tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca
     3361 agctgaaaaa caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg
     3421 tggttccacc taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc
     3481 ccttgcttca acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc
     3541 agcctagctt ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca
     3601 attatgtgtt tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt
     3661 tttccattcc ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat
     3721 gtgttctttt ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt
     3781 ttctaattgt tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct
     3841 ctctcctctt tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg
     3901 gtactgaatc aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga
     3961 ccgtatccac gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg
     4021 caattaacat caaaaaaata caaataaaga tgccaatcag tacagactgt ttcgctattc
     4081 tcatcgtatc tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga
     4141 tgaccgcatc tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga
     4201 tatctgcagc agcaagtgct ggtgcgtcat tcacgccgtc tcctaccatc gcaacaggtc
     4261 tttgatcttt agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat
     4321 gtacagaatc taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg
     4381 tgatcatcat caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt
     4441 ctgggcgaat ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat
     4501 agattgctgt attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct
     4561 ttacttcatg attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac
     4621 taacaggata cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc
     4681 tagattcctg ttctgcacta gctgcataca gaagtagctc ttcttttttt attccattga
     4741 ctggctgaat cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag
     4801 caatcgtttt tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt
     4861 ttttacttga acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac
     4921 aaggtgatgc cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag
     4981 aaataccacc gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac
     5041 ggacaaatcg ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact
     5101 ggctgtctgc ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc
     5161 ctgacataat ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag
     5221 attcatcgac cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag
     5281 gcttgactag taactggtca tcaatattta tttcatcgat tactatatcc tgcaattttc
     5341 cttctttgaa tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac
     5401 ccgctcgttt attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca
     5461 tcaaactagc ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata
     5521 catctacgcc atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg
     5581 cgagaatccc tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa
     5641 attcacttaa taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg
     5701 tcattgtttt ttctcctctt ttatgtgact tagagaggtt agtaataaat ttccagctgc
     5761 ctctttcatc ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga
     5821 aaacaactat cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag
     5881 caaggattac tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat
     5941 acttttaatt cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag
     6001 aacaaacgca cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata
     6061 caaaacaaca agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca
     6121 agattcgagc tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg
     6181 agcaggaatt gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt
     6241 tgtctgtcat gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa
     6301 cagaaacatt atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta
     6361 ttgactggcg cgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt
     6421 atgaaactga aacagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc
     6481 aagaaggacg tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc
     6541 tagagatatt ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa
     6601 aagcacaaaa tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg
     6661 ccggaagtgg aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc
     6721 gcaagtggct cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt
     6781 acatctcgac tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa
     6841 attatttggt gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg
     6901 gttttttgct aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg
     6961 atcagattga tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga
     7021 aaataaatgg ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa
     7081 atgaacaatt acctttacat catagacttt ctctattaca aacgaagctc ctgaaaaaat
     7141 taggcggtct gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac
     7201 agctccaaga actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt
     7261 cattacgtaa aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag
     7321 ggatcatgaa ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg
     7381 tgccactttc tcttctaaca cgacataaaa tcagcgagac caggtggcag gaacatttga
     7441 caactgttcg tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct
     7501 ttttactgat gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta
     7561 ttgatgaaat gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa
     7621 aagcaaattt gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg
     7681 ttggatcatt ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa
     7741 gctatcgctc cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc
     7801 aagtagaaac aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg
     7861 atatcaaatt gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt
     7921 ggcgtacagc aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc
     7981 cagacgagtt gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaagcgtt
     8041 ccattatttt acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt
     8101 gggatgtaga tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt
     8161 gtacacgtgc gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa
     8221 acaatatgga tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat
     8281 cattaatgtt agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat
     8341 catttaaggt catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga
     8401 caagcgaaat aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct
     8461 tgctatgacg gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga
     8521 attggtcaat tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata
     8581 cacgaaatgc taggtatgca ctatcgatca ctttattttt tttcgtgtct atcttctcat
     8641 ttctttcgat aagaattttt tcaggacgat gtttcaagct ccactctaat agtagaaaga
     8701 aagcctccat ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa
     8761 gcagcggaat cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact
     8821 ctaaataacg gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc
     8881 cgaatcttgc gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt
     8941 ctactttgcg gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca
     9001 tattcttccc cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc
     9061 atcataccac aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg
     9121 ttagtgagat tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct
     9181 tagtcttcta atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca
     9241 tcaatcattt tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg
     9301 atcatttgga tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt
     9361 atactatgga aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca
     9421 tcgcgaacat cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct
     9481 gcaaaagcaa agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg
     9541 ttcccgccat ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc
     9601 gtcaatgtat caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca
     9661 gagtatgttt catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt
     9721 ggtacattat aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga
     9781 tcaggatcac ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg
     9841 ctattattag catctttagc agttttcttt gctacgatat ttgcccattt acgtcccata
     9901 ttttcacttc gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata
     9961 cttgtgaatc acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta
    10021 gtcaattatc tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc
    10081 caattgatag atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc
    10141 gattcgggaa taaatagaaa tctctttttt ttcttttaag aatcttcttc cagcatcggt
    10201 aaatccaagt agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat
    10261 attcagcaag acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt
    10321 tacctttgac acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga
    10381 ttctattcct tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa
    10441 ccgataacgc aaatacggcc aataagattc ccaagtctgc agcgaacaag ttgaaagatc
    10501 ttcttctgtc tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg
    10561 aatggctgtt gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact
    10621 tttacgtcgg atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata
    10681 acttaatccc aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact
    10741 gcgaaaaacc tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa
    10801 tgcctcttct atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa
    10861 cgaagtctct tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt
    10921 tactgcacct ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac
    10981 tactaaatcc acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc
    11041 cggttcgcct ctttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt
    11101 tatttttcgt gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac
    11161 gataatcccg cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag
    11221 agacaagtaa aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc
    11281 aaacacaata cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga
    11341 tatataattt atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta
    11401 gtctttgtat gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa
    11461 atcagaaaat gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc
    11521 caatggatag gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc
    11581 ttgttcttca acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata
    11641 actatcccac aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaac
    11701 ctcatctact tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc
    11761 ttgatagact tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga
    11821 atcagaaaaa caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc
    11881 ttccacaaat tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat
    11941 ctcttctgaa aggtctaacc ctgtcacttt atatccatct tttgcaaagg caaccgctaa
    12001 tgctccagta ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt
    12061 cgaaaaagct aaccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc
    12121 aaatgtttca taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc
    12181 tgaccatagt ttttccagat tatagaaatt tcgttctggc gcatggaaaa catgtacgat
    12241 cacatctcca agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac
    12301 ttcatatttg ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg
    12361 ttcgctattt gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaacgtc
    12421 taaagcgata atgtcttctg cccgttttga atctgctgct tcaatcgcaa tttttaattg
    12481 ttctttacta tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag
    12541 taattatatg tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc
    12601 aatgtatgtt ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt
    12661 gcatcttgta ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt
    12721 ttgtcaagga gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatc
    12781 tctgcatcgg tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag
    12841 attgcattat tataattcaa taactccgga tcaaaaccgt ctcttacgat taccattttg
    12901 aactcctcat ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt
    12961 tcaggagaag caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca
    13021 tgcttgaagc gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa
    13081 taagcagtat attcattact atagtccata ttcatacaac ccttcatttt gaatataatc
    13141 aattacttta tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat
    13201 tttcgtggaa ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc
    13261 cgttgtgtat cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat
    13321 tttataccat tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt
    13381 atccggattg ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg
    13441 agctaattca atcgtttcaa tctgcaaaaa aggattgtct tccaccgcta attccaacat
    13501 gttgagtcga tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga
    13561 aggcatcaaa aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa
    13621 atgtgcaagg tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc
    13681 taagaattga ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt
    13741 attcatcatg gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc
    13801 ttttgtggaa ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc
    13861 tatttctttt tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa
    13921 cgatactttg atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga
    13981 attgatccca ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa
    14041 aaaacgcttt tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt
    14101 gcgtttcaat acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac
    14161 agtgatccag cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa
    14221 acgaacaagc tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc
    14281 tacatgtttt tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc
    14341 atttgacaca taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag
    14401 tccgcccaag aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt
    14461 taacggtgca atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg
    14521 gataattccc ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc
    14581 caaggtcgtt cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt
    14641 gatgatttga ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc
    14701 ccgtccatcg cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt
    14761 agcactagtt aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat
    14821 ccattgtgtt aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac
    14881 taaaagtact ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa
    14941 gtcaaaaata tccaccacat tgacgatcaa tgcatctttt tgccctaatt cattcagtaa
    15001 gcgcaaaaag tcgtcatccg tcaatgagac atcttgggtc tcattataat ggcgtaatct
    15061 gaaacaacgc tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg
    15121 cgtgtatcct aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg
    15181 taattcttct gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga
    15241 taggccataa ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg
    15301 ataatgggtt ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc
    15361 ataatctgat cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct
    15421 atgttgatcc ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt
    15481 tcaatcgctc gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct
    15541 gcatttttca tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat
    15601 gcaatcaacg tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg
    15661 gcaggtgtaa tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg
    15721 ctgtctcctc atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat
    15781 actgcatatc agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc
    15841 tttacaaaat gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact
    15901 aatattggcg gaacctcttg tatcgttttt ccagtaattt atgctgatct aatcgaacaa
    15961 gttccgtcaa tctttcaatc aacgcttttt tcataatgtg tgcgacttta tatcgtggca
    16021 actgctcgcc gttgaacgtt tcaggaataa ctcgatcaac gattcctaac tcgcgtagtt
    16081 ctggcgcagt aattttcatc aactcagctg cttctgcggc gcgactaccg tctttccaca
    16141 aaatcgacgc aaatccttct ggagaaagca cagcataaat cgtttcttcc agcatccaga
    16201 cttcatccgc taaacctaga gcaagggcac cgccacttcc accttcacca atgatgatgg
    16261 cgatgatcgg tacctttaaa tcagacattt cgagcagatt tctggcaata gcctctcctt
    16321 cgccgcgctc ttctgctcct acaccgcaat atgcacctgc tgtattgatc aaggtaatca
    16381 ccggtctatt gaacttttcc gcttgtttca tcagtcgtaa agcttttcga tagccttcag
    16441 gatggggaga accaaaattt ctgcggatat tttcctgcag atcccgtcct ttttgaatac
    16501 cgatcactgt taccggtttg ccatctaatc gagcgattcc accaacaatc gcttcgtcat
    16561 ctgcaaataa acgatcacca tggcattcct cgaaagaatc gaagatctct tcaaaaaaat
    16621 ccaagcttgt ccatcgttct ttagctcttg ctaactgaac gacatcatgt gctgtcttat
    16681 ccacgatcta tccatccttt ctgtgtatgc aattcaagaa gcgtatggat tttctgtttc
    16741 aattccatgc ggggaacaat ctgatcgaca aaaccatgag ataataagaa ttcagccttt
    16801 tggaaatctt ctggaagttc ctgcttgatc gtctgttcga tgacgcgtct tccagcaaaa
    16861 ccgatcaagc tttggggttc tgctagaata atatcgcctt ccattgcaaa actggcagtt
    16921 actcctccgg tcgtcgggtc cgttaaaaca gttagataaa aaagaccttc attgctatgc
    16981 cgtttgacag cagcagagat ttttgccatc tgcattagag aaaagattcc ttcttgcatc
    17041 cgtgcgccac ctgaagctgt gaacaagacg actggaagac tttcctttgt tgctcgttcg
    17101 aataatctgg tgatcttttc tccgacaatc gttcccatac ttcccatgat aaaattagga
    17161 tccatcactc caataatgaa gggaatcccc tgaatggttg ctttaccagt gaggactgct
    17221 tcatgaagtc ctgttttatc ttgcattttt ttaatttttt ccgtatattc gggaaatgct
    17281 agcggatctt tggttacaag atccgtatcc cattcttcaa aacttttttc atcaataatc
    17341 aaagctagac gctgccatgc accgattcga aaagcgtatc cgcaatgagg gcatactttt
    17401 tcttcaccga tgtctttcgt ataaaggata tgtttgcaat ttggacactt tgcccacatg
    17461 ttatcaggaa cagatggcga gttagcaggt ttgctctctc tattaggatt gatccgaata
    17521 tatttctttt tcttaaacag agccatctat gcgcctccta tctccgtttc tggtgtccaa
    17581 ttcggtaaaa attcttcttg taaaaatgct gtgctgtaat cacctgctat gactgcagga
    17641 tgactaatca aatccatctg aaattctgcg ttcgtgatga ttccttctgt gataaattca
    17701 cttaaggctc gctgcatctt cattaatgct tcaaaacgcg tatttccatg gacgattatt
    17761 tttgcaatca ttgaatcata atatggcggg atcgtatatc ccgggtaaac tgcactgtct
    17821 actcgcagtc ccatcccacc tgctggaagc agtaagtttt ggatcttccc tggagaagga
    17881 gcaaaatgga aagcaggatt ttctgcatta atccggcatt cgattgcgtg tccttggaat
    17941 actacttctt cttgacacac attcaaaggc tcaccggctg ctatctccac ctgttttttt
    18001 actaaatcga ttcctgtgac catttctgtt actggatgtt ccacttggat tcgagtattc
    18061 atttccatga aataaaaatc gccttcttca tccatcagaa actcgatcgt tccggcattt
    18121 tcataattga ctgctttagc tgctcgaaca gcagcgtctc ctagtgcttc tctttttgtt
    18181 tgtgagatca ctacagaagg agattcttcc agtacttttt gattgttgcg ttgtaaagaa
    18241 caatctcgct cgcctaaatg aatgacatga ccaaatcgat cacctaagat ctgaacttct
    18301 atatggcgag caggataaat gattttttcc aaatacatat catcatttcc aaatgccgct
    18361 gctgcttctt gctgagctga agaaaaatgc tgtgggagct cttcttttga ttgaactttc
    18421 cgaatgcctt tcccgccacc acctgcagct gctttcagca tcactggata accaattctg
    18481 tctgctattt ccaatgcttc ttctactgta gataatacac cagtacttcc tggaatgact
    18541 gggacatttg cttcttgcat caattgacgc gcattgatct tatttcccat tgcatcgatc
    18601 gttgcacttt taggaccaat aaatgtgata ttgcactctt cacacatgga ggcaaatcgg
    18661 ctattttcag ataaaaaacc aaatcctgga tgaattgctt cggcttttgt cacgattgca
    18721 gcgctcagta cttcctgaac attcagatat gaatcaacgg cttttgccgg gccaatgcag
    18781 attgcttcat ctgctaactg tgtatgaagt gcttcggcat ctgcttctga atatatcgct
    18841 acggtttgga tccctagttc gcggcatgca cgaatgatac gtacagcgat ttcaccgcgg
    18901 ttagctatca atactttaga aaacatattt cacctatcca atcataaagg ttaattccgc
    18961 ttcggctact tttttaccgt ctactttagc gattcctttg cctattccgg ctgaagcttt
    19021 cactttcaat atctcaactt caaggtataa ggtatctccc ggtgtaactt tcttacggaa
    19081 ttttgctttg tccaatccgc cgaaataagc cgtctttccc ttgaattgtt caagagaaag
    19141 taatgcaact gctcctgctt gagccattgc ttcaacaatc aatacccctg gcataacagg
    19201 ttcttctgga aaatgtcctt gaaaaaaggg ctcattgatc gttacatttt tcttagcaac
    19261 gatccgttct ccttcaacca tttcttccac tcgatcaatc aaaagcattg gataacgatg
    19321 tggaatgatt tcttttattt cttgtatgtt cattgttttc cctctttcct gctttgctta
    19381 tttttgctga atcctaaaga gtggctgtcc atattcgaca actgactcgt tttctacaag
    19441 gatttctgtg atcactccgt cggcctcact agttatctca ttcatcagtt tcatcgcttc
    19501 aataatgcag ataacttctc ctttggcaac ttgatcacct acttttttga aagctggttt
    19561 gtcgggcgct ggttgcagat aaacgattcc cacgattggc gaaacaacct cgattccatc
    19621 aaatactgcc gattcttttt ctttcattat ttctggagca gttgttgctt gttcaggtat
    19681 gacattgact ggctccgaaa ctggcaacgt ttcgtttgtt tgaaccatat tcatgcctcg
    19741 acttgtttta tttttattca tatacaattc aaattgacct tcacgcaaat cgaattccgt
    19801 tagcgaggat tgatcaaatt gactgactaa ttctctgatt tcattgatat ccaaaattat
    19861 tcctcccagc gtttcatgca cagaaccgca ttatgtccgc caaaccctaa tgaattactc
    19921 attgcataag taaaacttgc aggtttgctt tcgtttttga caactgtcaa gtcaatctca
    19981 ggatcttgtt tttctacatt gatcgtaggt gggataaatt gatgttctaa tgccagcaaa
    20041 gttgccaagg cttcgatccc gccggctgct cccaataaat gtccagtcat cgatttcgtg
    20101 ctgctgacgt aagtatctct gaaattttca cctaatgcga tttggattgc ttttgcttct
    20161 gcctggtcat tggctggtgt acttgttccg tgagcattga tatatccgac ttgttccggc
    20221 tgtacaccag cttcttccat cgcaagcttc attgctttag atgcaccaga accatctgga
    20281 gtaggagctg tcatatggta agcgtcacag tttgcaccgt atccaacgat ttctccatag
    20341 attttggcac cgcgagcttg tgcatgttct aaagattcaa gaactagaac gcctgctcct
    20401 tcacccatca caaaaccatt ccgattttcg tcaaacggaa tcgaaccacg attaggatca
    20461 gttgattctg agagtgctgt taaagctgca aatccagcaa tccccatctc acaaatcgtt
    20521 gcttctgttc ctccagcaag aatgacatca gaatagccat gtttgatgtt acggaatgct
    20581 tccccaattg aattcgttcc gctagcacaa gctgttacga tagctgtaca aatacctttt
    20641 gcacctacac gcaaagcaat atttccagct gccatgttgg caatcgccat tggtacgaac
    20701 attggagaaa ctcgttttgc acctttttca tgtagacgaa taacttggtt ttcgattgtt
    20761 ggaagaccac cgatcccaga tccgatgatg acgccaaacc ggtcttgatt cattttttct
    20821 ttatctaaac cgctcatctc cattgcttct aaagcagcgt agattccata gatcgaaaac
    20881 atatccattc gtttgctatc ttttttaata aaatatttat cgtaagggaa atctttgact
    20941 tcacccgcta aggtaatccc tgtttctgaa gcatcaaatt tagtgatcgg tgcgatgcca
    21001 ttcgtcccct cttttaagct attcaaaaag ctgtctgcgt catttccaat aggtgaagtg
    21061 actccatagc cagtaattac cactcgattc atttgtttgc tctccttttc ttatccgtgc
    21121 atgaccaatc cgccatcgac attgatgact tgtccggtga tatatggatt ttgtgctaag
    21181 aaaactgctg tttgtgcaat atcttctact tgtccgaaac gctgcatcgg aatttgcttt
    21241 tccgcttgtt ccttgacttt gtctgctaaa acttccgtca tatcagttgt gatgaatcct
    21301 ggcgcaatgg cattacatgt aatgcctcta gttgcagctt ctcgtgccac agattttgtt
    21361 aatcctacta cgccagcttt actagctgca taatttgctt gaccaatatt tccaatcaac
    21421 ccagatacac tagagagatt aatgatggcc ccttctcgct gcttcatcat ttttttcaat
    21481 acgtgctgcg tcatattgaa ggttccaatc aaattgatat ccagacattt tttgaaatct
    21541 tctgcatcca ttcgcatcac aagtttgtca tttgtgattc cggcattgtt tactaacaca
    21601 tggatcgatc caagcttttc ttctgcttct ttgatcattt gtcccgcttt ctcataatca
    21661 gagatgtcac cggaaactcc tacgcatttt acaccgaatg cttcgatttc ttctatcttt
    21721 tcttttggaa tctctccgcg accattcaaa atgatatttg ctccagcttt tgcaaaagct
    21781 aaggccatcg ctttgccgat tcctcttgta ctacctgtga taaatacatt ttttcctttt
    21841 acatccataa cgttcctcct tatagtattg ctattgtttc atcaaatgtc tgcttattct
    21901 caacacgcag aactggtatt gttttatcga tttttttcat aaatccgctt aatacttttc
    21961 cggggccgac ttcgatgact tgttcgatgc cgattgtttt taacttatgg atactctcat
    22021 agaaacgaac aggttgcatg acttgctgtt caagtagtcc cgcgattgtt tctttcttca
    22081 ttatttccgt tgttgtgttg cttatgatag gaaaagaagg ttcagagaat tgaatctgac
    22141 ttaggtcctt cgccagtttt tttgctgccg gttctaaaat agctgtatga aaaggcccgc
    22201 tgacatttag agggatcatt cttttgaacc ctttttcttt taatagtaaa acagcttcgt
    22261 ctactgcttt ttcttcgcca ccaatcacga tctgctgagg tgtattataa tttgcaggag
    22321 atacgatgcc gtacttgcta gcttcatgac agctttcctc gatcgtttca atcggcgcat
    22381 tcatcactgc aaccattttc ccgcttccag ctggggcagc ttctgtcata taagcgcctc
    22441 tttttgctac taacgcaact gcttcagtga aggatagcgc attactagct actaatgccg
    22501 aatattctcc taaactcagt cctaaagctg catctgggat gattccatgc tccttcaata
    22561 agcgataata cgcgatgctg actgttagaa tagctggctg tgtatattgc gtctgatcca
    22621 aacgttcatt ttctgtaaag caaagttcag ccatatcgta gccgagaatc tcacttgctt
    22681 catcaaatgt ctgctttaca acggcttctt catataaatc ttttcccata ccttgatatt
    22741 gtgcaccttg gccactaaat aagaatgctg ttttcatgtt tttctccaat atatccagaa
    22801 tatatgtttg attcttccta ttcaaaaagg ccgagacatt cctatttcag gaaatgtccc
    22861 agcccccaac ctaattttaa tgattatgca gtttgttttt ctacgtaagt cactaaatct
    22921 tgaaccgttt tgattccgtc ttctgtttcg attttcacgt caaattcatc ttcaatttca
    22981 ttgatgattt ggaataaatc caagctgtca gcttctaagt cttcttggat gtcagtagtc
    23041 aatttgattt cttcagtttc ttttcctaat tcttctgcga tgatttcttg aattttttcg
    23101 aataccataa taatatattc ctccgatatt gtttgctttt tatttttata atttaacagt
    23161 ctacaactcc atgagaatac ttccccatgt taaacctccg ccatagcctg taaatattac
    23221 tttctgctgt tttcctaagg tgatctttcc actagaaact gcttcatcca ataagatagg
    23281 gatgcttgca gcagatgtat tcccatattt atccatattg gtcaggaact tttcttgcgg
    23341 catctttact tttcgggcaa ttttctcaat aattctgagg tttgcctgat gcaaaagcag
    23401 ataatcaact ggtttgtctt tcatgacttc ttggatattg tttgcaacgt ctcgtacggc
    23461 aaaatcaaaa atatctctgc cagtcatttg caaataatac gaaccttctg aatcttctga
    23521 atagaacgga tttttatttt gctggtagcc agcggtcaat gagctacttc tggtaccgtc
    23581 agctgctaat ttttctttca gcaatttttt ctgtggattc gcttctaaga gaatcccacc
    23641 tgctccatcg ccaaacaaca cagcagtcgt acgatcagac caatcgatta gcttagatag
    23701 tgtttcacca ccaatcacca gtcccttagt tgagccgcag cggatcattt tttcagccat
    23761 gcttaacgca taaacaaatc ctgagcacgc agcactgata tcaaaagcga tagcatgagt
    23821 tgctcccaat ttcccttgta cttgtgctgc tacagatggt gtcgtgtaat cgggcgacat
    23881 tgttgctact aaaataaaat ccaaatcttt agggtttaga tttttctgtt ttatcaattt
    23941 ttccgccact aaaatgcata aatcagaagt ctcctgattt gttacacatc ttcgttcacg
    24001 aatacccgtc cttgacgaaa tccattcatc agaggtatcc atcatcttgg aaagatcatt
    24061 attagtaata atcttttcag gaaggacgct ggctgttgca gtaatcattc caaattgttc
    24121 cattaaatat cctcacttat agtcttgcaa gaagtcgtgc aagtttttca gtgcatccag
    24181 caaggcatgc tgttcctccg tagccattcc gtctaagatg ttcttgacca tttcacgatg
    24241 aaaatgctga tggacccgaa atagtaactt tccttttttc gttagtccca actttacaac
    24301 gcggcggtca tcttcactgc ggatacgttc gacatagcct ttttttacta acttgttaat
    24361 agcagtggtc aaggtcccaa cagtaattga tagttctttg gctacttctg aagttgtttt
    24421 tttcttgtac atcccgattg cctcgatcgt gtgcatctct gtgattgaca gatctttgaa
    24481 ttgagatttt ttcagctcag actcttcgat tgtcaaaata tcattgaaga cactaacaag
    24541 ataatcattt actgttttta aatctggttc catgttccac cgtccactaa ttagtttgat
    24601 tatcaaatct tttgatgttc aaactatatc ttaacttttt tcaaaatgca accttaaaag
    24661 atcatttttt tactatatga gcttttttta agattttact catttaaaac aactgataaa
    24721 tcagcattta taatttcttt tattagaaaa atcatctatc tcacatattt tgattatcaa
    24781 agtattttct gtgaacagaa ctatagaatt ccctaaaact tgtccattat ttcaaaattt
    24841 gttatagtct ttttattcaa agcgtaaaag gagatcctat gttaaaagaa aaaatgaaaa
    24901 ctctccccat gctgccgggt gtctatctta tgaaagaccg agatggtaag gtcatctatg
    24961 ttggtaaagc aaaacggctc aaaaatagag taagcagcta ttttcatcag aataaacagc
    25021 attcaaaaaa agtattacgt atgattcatc atataacaga ttttgatttc gtcgtagtag
    25081 acacggaatt agatgcttta ttgctcgagt gtcaattgat ccaacattac cgtccttttt
    25141 ataaccgaca aatgaattat ttcagtaatt ataattatgt acatattacc aataagggat
    25201 ttgtattaac agatactcct acggctcgaa catacgggcc ttttcgtctc tataaaaaaa
    25261 tgccgtctat tttgcgtata atggaagaaa cttatcaaat gccttggttg tctgaaatta
    25321 gtttgttagc tttgcgagtc cagcttcctg acctacagga aatgactttt gaacagaaaa
    25381 aaaaggaact ccaagggctg tttcaaggcc gaaataaaaa gctactgact tacttgaaaa
    25441 aaagacaaca acatttcatc tatcagttga atttcgaaaa agccggtatg cttcaaaaag
    25501 atatcgaatt agttacttat tttattagac ggatccagga acaaaagcag tttttaagaa
    25561 caccatcgct caccttcagc atgccattag cagcagatga gagtcagaaa aagcactacc
    25621 tcgtttgtta tggtcaactt gcagaaacaa tgattacctc tggtgacgtt tctcctgact
    25681 tctattatga aaagaaagaa gctcatttat ctttgaaacg tcaattgtca aaagaagaaa
    25741 tcgatcctgt acaaatcttg atcagttacc gcaaaaaatt agaaaaagaa cagtttgaaa
    25801 ttgaactgtt aaacaaaaaa gaagctgaaa agcaactaaa ttagttgctt ctcggcttct
    25861 tttaggcatc ttcaacttca aatgtcaatt atcctaaaat ttattggatt aagtcataga
    25921 tttccatcgc tacgatatcg atattatcaa attcgtattg ctgatgagta tctgtttcag
    25981 ttaattcaaa tgtatcggtc gctttatcat aagttactgt acacttctct acgccttctt
    26041 tttcaaaacg gcgaacttgc agttcattat ctgctgactc gaccatagcc tccagtcgtt
    26101 ttatgattgc gactaattgt gaatgtgtca tcggaggtcc tccttttctt atttctgtta
    26161 tattgtatca aaagcatagc ctacttgcat tgattttctc taaaaaaact cgttttttat
    26221 gatttttttt ctggtatttt tccttcaggc caccaaagtt tgatcaaagc ttgggttccg
    26281 tcattctcaa aacctttatc tgccaactct tcatatagtt tcaatgcttg ggtagttgct
    26341 ggcaatacaa gatccatttt ttctgcttct tctaatgcaa tctttaaatc tttgataaaa
    26401 tgtttgacaa aaaaaccagg tgtgtaatct tcttttaaaa tacgaggaga ataattgctt
    26461 aaagaccagt tagctgcact tccacctgaa agcgtctcaa tcactttctt taaatcaagt
    26521 ccgttttttt gtgcatagac aagcatttcc gtcattcctg tcatcgtacc tgcaatcatg
    26581 atctgattag ccatttttgt atgctgacct ttcccagcag aaccatgcaa tgtaaatgtc
    26641 gtaccaaatt ctttgaataa aggcagtact tgatcataaa cggcttcttc tccaccgacc
    26701 atgatcgtca gtgtaccatt ctttgctcct aagtcaccac cggataccgg tgcatctaac
    26761 gcatctgcgc ctttttcttt tgctgttttt gcaatttttt ctgccaggct tggtgtactt
    26821 gtagtcaaat caactaaaat atgtcctgag atatctgcag aaaaaatccc cgactgtcca
    26881 tagtagattt cttctacatc tttggggtaa cctaccattg taaagagaat attactttgt
    26941 tctgctaccg cttttggtgt atctgcccag atagctcctt ctgttactaa aggatcagct
    27001 tttgcttttg tccgattata gacagctact tcgtgtcctg cttctagtag atggcgtgcg
    27061 acagcacttc ccatgacgcc tgttccgata aaaccaagtt tcattttttc gacctcctaa
    27121 aaatcaaaaa tccttgcttc tatgctagta tacgctagaa acaaggattt tttcataaaa
    27181 aatgtttgaa ttttatcttt taaatgaatt taattttatt ttgattcgtt caatcgagtc
    27241 ttcttcttct tttcttcgca tctcttcttc atgataaaca accatgtctt gataaaattc
    27301 gatcatagct tcgccgaaat gtgtagatga aatatcataa agtttagctt ctaaaacttt
    27361 tggatcttta ggaacttcac tggaaatata atcaataagt gctggtgcaa agtcttcgtc
    27421 ggtttcaaaa gttttcccta atgaaggatg atcgaacaat cggttcaaat aagcatttcc
    27481 ttccacgaca caaggtgttc cggctgccat cgcttctgta taagtaagcc cttgtgtttc
    27541 tgaagtagaa gcactgacaa aataatctgc cgctttataa taaagtgcta catcattgtt
    27601 gtccacttct cctgtgaact gcacaaattc ttctaactgc agttctgaga tcaattcctc
    27661 taactcttct acataaggtc cttttcctac gataactaat ctggcgttag gataagcgga
    27721 gatgacctga ggaaacccat taatcaaggc ttggatattt ttctcataag aaatcctgct
    27781 taaagaaaga atcatcaaat ggttttcctg aagacctagt gactccctta atttgctgat
    27841 atcctgctga gtgatatcag aacgtttgaa tttttcaatg tcgataccag taggaatcac
    27901 gcgaagcggt gctattacac catagctgcg taatgtatca ataacacgtt cacttggaca
    27961 aactacacct gtcgtatgat ttgcaaataa tctagacaag tattttacat gggattgcct
    28021 gatcactttt cctttaccga tataatgaag gtagtcttca tacatcgtat ggtaagtatg
    28081 gatcagaggt attttcaact tcttagctac catcttccca agtatccctg ctccgaattc
    28141 tgtatgggta tgaataagat ctagttctag ttctttggca accaaataag cataccacat
    28201 gcctctaaca acaactcttc gatccttgaa agaaataaat ggtacacttg gcattcgaat
    28261 cacatctttt tccaacttat ccgcattagg atcggtcgtt gtaaaaatat agacttcatg
    28321 cccatgtctt tctaattctt gttttaatgt tttgatcgat gtagcaactc cgctgacttg
    28381 cggaaagtag gtatcggtaa aaaagccgat tttcatgttt ttcgcctcca aactagtcct
    28441 tgacctgatt tacttttttt aatttgctag agactttttc attcagtaca ctttcgtata
    28501 catttttaag ttcttcaccg attttattga tacttcgctc ttttgctgtt tgataaccgg
    28561 cttgtgtcgt actcggtagt tcttgattgg taatctgttg gatcagtcgg ataaattctt
    28621 cgtttgtctt ccccatatag cagttcttgc tatcttctaa ccagccatta tacactggaa
    28681 tatctcgaac aagtacgttt tgtttgcttg ctaaagcttc taaaacaacg atcccttctg
    28741 tctcttcata agaagggaaa aagaatagat ctgcacctga atacgcacct tctatgatct
    28801 ctccacgaat gtaaccagga aacaggacat tttcaggatg atcttctttt acaattttcc
    28861 ggatgttccg aggaatcgag tacatgggaa catgaccaaa ccaaataaat gtgtattcgg
    28921 gcatcttctt agcaatttct acgaaatcaa tgattccttt acgttcgaaa aacaaaccga
    28981 cacaaatgat tactttttga tccggagaca gtttgaaata ttctctgaac ttttgttctt
    29041 tcattggatc agggtgatat ttctctaagt cgatcccatt ggaaatagct tgaatcggca
    29101 cacggatacc atagctttcc aacaactgtt ttgaataagg tgttggcgtg atcagatagt
    29161 ccgctttttg gtacaatcct accagatatt tttttacgat aggagaaagc tggttcgatc
    29221 cgataaagga atttcggaaa tcttcctctg tagaatgcgc atgatagaca actttttttc
    29281 cttctcttct ggctttattg accatattat gactattgat tccgtatgta ttgatgtgca
    29341 ggatgtcata atcttctttt tcatctagtg tataagagat accgacttcc gtcagagcac
    29401 gtttttgatg atcaagcgca cgcccgattc cagattttgc aagcatcttt tcactttcaa
    29461 aatataataa taccttcacc ttacgtcccc cttcacttat atagtataac atagcctaca
    29521 aactgtcact caaacttaag ccctattt
//
LOCUS       DASJPC010000027        28150 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJPC010000027 DASJPC010000000
VERSION     DASJPC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.027"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(205..1104)
                     /locus_tag="U4A13_001173"
     CDS             complement(205..1104)
                     /locus_tag="U4A13_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN1779483.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="U4A13_001174"
     CDS             complement(1114..1470)
                     /locus_tag="U4A13_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1779484.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="U4A13_001175"
     CDS             1825..2121
                     /locus_tag="U4A13_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN1779485.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2765..3718)
                     /locus_tag="U4A13_001176"
     CDS             complement(2765..3718)
                     /locus_tag="U4A13_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1779486.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3741..4496)
                     /locus_tag="U4A13_001177"
     CDS             complement(3741..4496)
                     /locus_tag="U4A13_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1779487.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4493..5452)
                     /locus_tag="U4A13_001178"
     CDS             complement(4493..5452)
                     /locus_tag="U4A13_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1779488.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5445..6395)
                     /locus_tag="U4A13_001179"
     CDS             complement(5445..6395)
                     /locus_tag="U4A13_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN1779489.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6545..6997
                     /locus_tag="U4A13_001180"
     CDS             6545..6997
                     /locus_tag="U4A13_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN1779490.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="U4A13_001181"
     CDS             complement(7086..9353)
                     /gene="gshAB"
                     /locus_tag="U4A13_001181"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN1779491.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="U4A13_001182"
     CDS             complement(9540..10538)
                     /gene="ftsY"
                     /locus_tag="U4A13_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN1779492.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10549..11358)
                     /locus_tag="U4A13_001183"
     CDS             complement(10549..11358)
                     /locus_tag="U4A13_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1779493.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11367..14948)
                     /gene="smc"
                     /locus_tag="U4A13_001184"
     CDS             complement(11367..14948)
                     /gene="smc"
                     /locus_tag="U4A13_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN1779494.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14968..15654)
                     /locus_tag="U4A13_001185"
     CDS             complement(14968..15654)
                     /locus_tag="U4A13_001185"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN1779495.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16002..17783)
                     /locus_tag="U4A13_001186"
     CDS             complement(16002..17783)
                     /locus_tag="U4A13_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1779496.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17809..18723)
                     /locus_tag="U4A13_001187"
     CDS             complement(17809..18723)
                     /locus_tag="U4A13_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779497.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18750..19712)
                     /locus_tag="U4A13_001188"
     CDS             complement(18750..19712)
                     /locus_tag="U4A13_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779498.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19712..20656)
                     /locus_tag="U4A13_001189"
     CDS             complement(19712..20656)
                     /locus_tag="U4A13_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779499.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20657..21661)
                     /locus_tag="U4A13_001190"
     CDS             complement(20657..21661)
                     /locus_tag="U4A13_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779500.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="U4A13_001191"
     CDS             complement(21929..22171)
                     /gene="acpP"
                     /locus_tag="U4A13_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN1779501.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="U4A13_001192"
     CDS             complement(22293..23294)
                     /gene="plsX"
                     /locus_tag="U4A13_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN1779502.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23343..25379)
                     /gene="recG"
                     /locus_tag="U4A13_001193"
     CDS             complement(23343..25379)
                     /gene="recG"
                     /locus_tag="U4A13_001193"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN1779503.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25496..27178)
                     /locus_tag="U4A13_001194"
     CDS             complement(25496..27178)
                     /locus_tag="U4A13_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN1779504.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27230..27592)
                     /locus_tag="U4A13_001195"
     CDS             complement(27230..27592)
                     /locus_tag="U4A13_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1779505.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27862..28050
                     /locus_tag="U4A13_001196"
     CDS             27862..28050
                     /locus_tag="U4A13_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN1779506.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7783 a         6086 c         4956 g         9325 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtgggc ttattgccga
     2281 gaagcagcag atggaacatc gtttagaaga gattgtgaca actgcgattt gctttgagta
     2341 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2401 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2461 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2521 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2581 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2641 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2701 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2761 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2821 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2881 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2941 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     3001 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     3061 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3121 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3181 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3241 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3301 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3361 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3421 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3481 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3541 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3601 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3661 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3721 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3781 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3841 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3901 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3961 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     4021 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     4081 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4141 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4201 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4261 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4321 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4381 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4441 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4501 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4561 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4621 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4681 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4741 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4801 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4861 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4921 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4981 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     5041 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     5101 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5161 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5221 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5281 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5341 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5401 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5461 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5521 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5581 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5641 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5701 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5761 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5821 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5881 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5941 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     6001 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     6061 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6121 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6181 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6241 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6301 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6361 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6421 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6481 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6541 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6601 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6661 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6721 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6781 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6841 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6901 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6961 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     7021 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     7081 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7141 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7201 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7261 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7321 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7381 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7441 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7501 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7561 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7621 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7681 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7741 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7801 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7861 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7921 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7981 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     8041 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     8101 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8161 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8221 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8281 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8341 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8401 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8461 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8521 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8581 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8641 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8701 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8761 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8821 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8881 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8941 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     9001 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     9061 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9121 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9181 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9241 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9301 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9361 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9421 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9481 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9541 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9601 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9661 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9721 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9781 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9841 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9901 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9961 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
    10021 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
    10081 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10141 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10201 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10261 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10321 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10381 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10441 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10501 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10561 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10621 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10681 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10741 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10801 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10861 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10921 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10981 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    11041 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    11101 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11161 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11221 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11281 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11341 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11401 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11461 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11521 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11581 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11641 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11701 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11761 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11821 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11881 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11941 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    12001 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    12061 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12121 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12181 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12241 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12301 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12361 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12421 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12481 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12541 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12601 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12661 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12721 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12781 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12841 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12901 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12961 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    13021 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    13081 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13141 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13201 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13261 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13321 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13381 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13441 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13501 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13561 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13621 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13681 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13741 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13801 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13861 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13921 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13981 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    14041 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    14101 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14161 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14221 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14281 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14341 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14401 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14461 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14521 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14581 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14641 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14701 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14761 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14821 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14881 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14941 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    15001 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    15061 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15121 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15181 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15241 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15301 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15361 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15421 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15481 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15541 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15601 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15661 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15721 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15781 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15841 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15901 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15961 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    16021 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    16081 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16141 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16201 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16261 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16321 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16381 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16441 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16501 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16561 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16621 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16681 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16741 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16801 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16861 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16921 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16981 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    17041 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    17101 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17161 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17221 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17281 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17341 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17401 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17461 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17521 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17581 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17641 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17701 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17761 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17821 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17881 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17941 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    18001 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    18061 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18121 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18181 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18241 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18301 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18361 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18421 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18481 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18541 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18601 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18661 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18721 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18781 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18841 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18901 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18961 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    19021 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    19081 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19141 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19201 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19261 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19321 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19381 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19441 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19501 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19561 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19621 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19681 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19741 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19801 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19861 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19921 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19981 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    20041 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    20101 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20161 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20221 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20281 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20341 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20401 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20461 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20521 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20581 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20641 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20701 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20761 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20821 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20881 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20941 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    21001 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    21061 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21121 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21181 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21241 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21301 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21361 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21421 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21481 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21541 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21601 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21661 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21721 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21781 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21841 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg ataaaaagct gcatgcagct
    21901 ttttatcctt ctttgctttt atttgaaatt atttaagatg gctgctgata taatctactg
    21961 cgccaccgac tgtttcaatt tgttcagcgt cttcatctga aatttccgta ccgaattcat
    22021 cttcaagctc taaaacgaat tccataatac taattgaatc agcttttaaa tcatctttga
    22081 tgttcaattc atccgtcact ttatcggtat ctatctcaaa atgatttgaa ataattttcg
    22141 ctactttgtt aaatacttct tcgcgagtca actttttcac ctccaagaat catacacttg
    22201 tcaattacct acaccatggc taattttact ggcatttttc aggaatgtcc accttttttt
    22261 caatggaacg gatcgcttca gatatccaaa tactattctt cgcttttttc aaattgttcg
    22321 actaattgtc caactacatc tgtctcaagc attgtgtgga tttggcggat cgtatagcga
    22381 acagcatcgg ggcctgttgc tccatgagtt ttaatgactg gtgctttcaa gccaaacaaa
    22441 acggcaccgc catgtttgga ataatccatt tctgctttca aagaacgtaa gccgtctttt
    22501 aaaagcatag cgcccatttt tcctttgatc ccttcattta gaatagcaga ttttaataga
    22561 ttcatcatat tcattgctgt tccttcaata gatttcaaaa cagcgttgcc tgtaaatcca
    22621 tctgtgacca ctacatcagc cacaccattg agtagctcac gagcttctac gtttccaata
    22681 aaattcaagc ttgtttcatt ctgaaggagt tcataggctt ttttcgtcac ttcactacct
    22741 tttgttgctt ctgttccgtt gtttaagagt gcgacacgag gacgtccgat acctcggact
    22801 ttacgtgcat aaaaagaacc taaaataccg tattgcaaca aatgttctgg tttgttttct
    22861 gcgttagcgc caagatccag catatcgaaa ccggcacctt ctttccctac tactggcaag
    22921 gtagacatca agccagggcg ttcgatattt ttgatacgtc ctacgatgaa caaccctgct
    22981 gctagtagcg cgcctgtatt ccctgctgaa aatacagcat ctgcttcacc gtttttcact
    23041 gcttgtgcag caaggaccat tgatgcagtc ttttttcgtc gaattgcttt tactggctca
    23101 tcatcactat tgattttttc gtctgtatgg ataatcgtga tgtttttttc atttgttaag
    23161 tatttcttga tttctgcttc ttttccgtaa agtaagaatt ctacttctgg aaaatcttgc
    23221 tgcgctaaag ccacgccttc tacaattgct tgaggtgcgt gatctccgcc cattgcatct
    23281 acggcaattt tcattgtttc cactcctttt tccgtcatca gtataaagta tagcacagtc
    23341 tgctaatcga aataacgatt atcctgattt tctatttgga ggctttgcgc taaaggctga
    23401 tactcaggaa gcatctgcca atcctttatt tgccaaaggg ctgcagcctc ttctctggct
    23461 acctccaaaa catttccatc cgtcaccaag tcagcaatcg caaattgagg cagaccagat
    23521 tgacggaagc caaatacttc accaggacca cgtaattcca gatctttttc gcttagtaca
    23581 aaaccattgg tggtctctgt catgattttc atccgttcca cacctagttc atttttagga
    23641 ttagcaacta agatacagta ggaagcatca cttccgcgtc caacacgtcc acgaagttgg
    23701 tgcagctgtg ccaatccaaa tcgatccgca tccatgatca gcatgacggt cgcgttcggc
    23761 acgtttaccc ctacttcaat tacagtagtc gaaactaata tctgcatctg gttgtctttg
    23821 aaggcttcca tgattacttc tttttcctga ttcttcattt taccatgtaa caagccaact
    23881 tcatattttg gtgcaaatag ttcacgaagt ttttcataga tttccactgc attttttaca
    23941 tctaatgctt ctgattcttc aatcagcgga cagatgacat acatttgatg tcctcttgcc
    24001 agttcttttt tcgtccaatc cagtacactg tccaattgag gtgttcttac ccagcgtgtt
    24061 tcgatcggga tacgtccagc tggaagttca tcaatgatag atacatccat ctcaccatat
    24121 gctgtaatag ctaatgttct aggaatcgga gtcgctgtca taaataagac atctggtcgc
    24181 cagccttttt cacgaagtac tttccgctgg ttcacgccaa aacggtgctg ctcatccgta
    24241 ataactagac ctaaattctg aaattcgact ccatcttgga tcaatgcatg tgtgccgatc
    24301 acgacgttaa tttccccttg ctctagtttt tcaaggagct cacgtcgttc ttttgctttc
    24361 gttgaccccg taatcaatgc gatggttact tcattgggat caaacagctg ttgcaagctt
    24421 tccatatgtt gttgtgccaa gatttctgta ggcaccatca acgcaccttg gaaacctgct
    24481 gtaacggtag cgtataacgc aatcgcggca accactgttt ttccgctacc tacatctccc
    24541 tgcaaaagtc gctgcatatg ctgcggactg cgaagatccc gacagatttc attcgttaca
    24601 cgtttttgtg catttgtcag ttcaaaaggt agcttttgag taaattgttt caatttttgg
    24661 acatcataaa ggatctccaa accattcgtt tctgcttttt ctgctttttt caatccttga
    24721 agacgcattt gaaataaaaa gaactcttca aaaacgacac gtcgtttggc ttgatggttt
    24781 tcttcgtggt cttttggaaa atgcatactt cttacagcag aagctcggtc aagcagtcga
    24841 tatttctcca caaggttgtt tggcagattt tcttcaatca atgtgccgta ttcttgaaac
    24901 ccttttttga ttagatcaac taatgtcgtt tgacgaattg atttgttgac gtgatatatc
    24961 ggcgaaaagt catccattgt ttgagaacct aaaatcttca tcccgttcaa ggctttacgc
    25021 tttgcatccc atttaccgta tacggcgatt tcctccgata aaatgacttt ttcttttaga
    25081 taaggttgat taaaaaaaga aacgttgaac acatcatgtt cttgcatcat acgaaattgc
    25141 aatcggcttt ttttataacc aaatcgattc atcacaggag gcgagactac gattcctttc
    25201 aaagtaacct tttcttgatc ctggatctcg tttagatttc tttcctgaat atcttcgtag
    25261 cgaaaaggat aataagtcag taagtcttca atcgtttgga tgcccaagct ggcaagacta
    25321 gctgctcttt tttctccgac tcctgccagc atagtgattg gatcatctaa agaacgcata
    25381 aatttttcct ccctccttta acaaaaaaag gacttggtca gcgatcatac ctgatcacta
    25441 accgaaggcc ttttatgaat gaatactgtg ttagaaagat attctgacac agaaattatt
    25501 cagcagaaaa tagataagga tagacaggct gatctccttg gtggatctcg atttccaaat
    25561 caggatgttt ttctgttagt ttctcagcaa attcatttgc ttgttcaact gttccttcat
    25621 cacctgccaa gatcgttacg atctctgtgt cctcattgat catttgttct agtgtactta
    25681 acgctgttgc tgtgatatct gggttagaga tcaggatttt tccatcgatc atgcccaaaa
    25741 agtctccttc tttgatctct acaccgtcaa ttgaggtatc tctgatcgcg tgagtgactt
    25801 gaccgctcac aacgctagac aacatttctg tcatcgtttc tttgttttct tctaaagaag
    25861 cttgatcatt gaatgaaagc atagctgtca atccttggga aatcgttctt gaaggaacaa
    25921 ctgctactgg gatatctgct acttctgctg cttgatcagc tgccatgaag atgttcttat
    25981 tgtttggcaa gacaatcact ttatctgcgt ttacttcttg aatcgcttta acaatgtctt
    26041 ccgtacttgg attcattgtt tgtccaccac tgataacata attagcgcct aaactttcaa
    26101 acagctcttg gacgccttta ccagcagcga tcgctacgat accatacggg atacgtggta
    26161 tttctttttt cgcaaactcg ttcacttctt catcgtgttc caaaatcgtc tcatgctgca
    26221 agcgcatgtt gtcgactttg attttgacta atgaaccgaa tttttgtcca taattcaaga
    26281 cttctcctgg gttttctgta tgcacgtgga ctttgatgat ctcgtcatca ttgaccacaa
    26341 gtaaagaatc tcctatacca tctaaatagt tacggaattc ttcgtaatcg aatgtactat
    26401 cgactgtagg tccttctccg atttttacca tgatttctgt acagtaacca aaatggatat
    26461 cttctgtcgc catttgacct tggacactgc gatgatgttc tgcattgacc atatcatcca
    26521 tttctgctgg agaaggttca tatgtttcat ccgcttggaa ttctccattc aatgcactta
    26581 aaaagccctc ataaacaaac agcagtcctt gccctccact gtctacgaca ccgacttctt
    26641 ttaggactgg aagcaagtct ggagttttag ctaaagcacg ctttgctcct tttacaacag
    26701 ctgtcattac ttcaacgaca tcatcagtac tttttgcttt cttttctcca gctttcgctg
    26761 cttctctagc tactgtcaaa atcgttcctt ctactggttt cattactgct ttatacgcag
    26821 tttcaactcc atgagtgaag gctgcagcaa gatcagaagc atttaatgag acaacatctg
    26881 gaatttgctt agaaaatcca cgaaacaatt gagacaaaat aacgccggaa tttccgcgcg
    26941 cacccatcag caatcctttt gaaagcattg ctgctaattc tcctactttt tctgaggttg
    27001 aatctactac tgccttagct ccactagtca tagatagatt catatttgtt cccgtatcgc
    27061 catctggcac agggaataca tttaatgaat tgacgtattc tgcatttact tgcagacgat
    27121 tcgcacctgc ctggaccatt tcctggaact ggcttgcact tatttctgtt acactcacct
    27181 agataatcct ccttcagatc gactgctgag ttaccagcca gtctcctgcc tagtctggca
    27241 gtacacgaac accttgtaca aagacattaa ctgaattggc agttacgcct aataatgcct
    27301 ctagattgta tttcactttt tcttggacgt tgcgggaaac ttcagagatt ttcgtaccat
    27361 agctgataat cgtataaaca tctacagcaa taccattatc ttcttggcgg acaactactc
    27421 ctcttgagta attttcttta cgcaaaatct cagtaatatt atctttaatt tgatttttgc
    27481 tggccattcc tacaacaccg aacacatctg tcgcggcgcc accaacaact gttgcaatta
    27541 catcattggt aatttcaatg gttccagctg acgtttgaat ttttacagcc atgaatgagc
    27601 ctccttaacg ggtaaaaaac tcccattttt cttctatcat attttatcat agcgcaactt
    27661 gaaataaaag gcagttaccc aaagtactca aaaaccttgc aaaatttcag gtataacctt
    27721 gtttaattta caattattct tctcaattgt caattgtatc tatgaaaatg tacttgcaaa
    27781 ttcccattct atatgatatg ttagtctggt atgagctgaa ttattctatt gctccaaatc
    27841 aaggcaaagg aggaaattac catggcaaaa gtttgttact ttacaggtcg caagacaaaa
    27901 agtggaaaca accgttcaca cgctatgaac tcaacaaaac gtactgttaa acctaactta
    27961 caaaaagttc gcgttatggt cgacggaaaa cctaaaaaag tttgggtgtc aactcgtgct
    28021 ttgaaatcag gaaaagttga acgcgtttaa ttgaatgata cttggaccgt tcagatagtg
    28081 aacggttttt tatttaataa aaatttttaa gggtgggact tttgattcct accctttttt
    28141 tgcgtttatt
//
LOCUS       DASJPC010000028        28064 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJPC010000028 DASJPC010000000
VERSION     DASJPC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.028"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            8..649
                     /locus_tag="U4A13_001197"
     CDS             8..649
                     /locus_tag="U4A13_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779507.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="U4A13_001198"
     CDS             708..1148
                     /locus_tag="U4A13_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779508.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="U4A13_001199"
     CDS             1160..1828
                     /locus_tag="U4A13_001199"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN1779509.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="U4A13_001200"
     CDS             1892..2515
                     /locus_tag="U4A13_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN1779510.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="U4A13_001201"
     CDS             2571..2957
                     /locus_tag="U4A13_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN1779511.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="U4A13_001202"
     CDS             3170..4561
                     /locus_tag="U4A13_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN1779512.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="U4A13_001203"
     CDS             5172..5540
                     /locus_tag="U4A13_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN1779513.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="U4A13_001204"
     CDS             5720..6661
                     /locus_tag="U4A13_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN1779514.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="U4A13_001205"
     CDS             6730..7461
                     /locus_tag="U4A13_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN1779515.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="U4A13_001206"
     CDS             7605..9785
                     /locus_tag="U4A13_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN1779516.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="U4A13_001207"
     CDS             9805..10287
                     /locus_tag="U4A13_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN1779517.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="U4A13_001208"
     CDS             10303..11064
                     /locus_tag="U4A13_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1779518.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="U4A13_001209"
     CDS             11195..12529
                     /locus_tag="U4A13_001209"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN1779519.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="U4A13_001210"
     CDS             12836..13321
                     /locus_tag="U4A13_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN1779520.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="U4A13_001211"
     CDS             13314..13997
                     /locus_tag="U4A13_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN1779521.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="U4A13_001212"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="U4A13_001212"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN1779522.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="U4A13_001213"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="U4A13_001213"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN1779523.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="U4A13_001214"
     CDS             17613..18242
                     /locus_tag="U4A13_001214"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN1779524.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="U4A13_001215"
     CDS             18294..18626
                     /locus_tag="U4A13_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1779525.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="U4A13_001216"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="U4A13_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN1779526.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="U4A13_001217"
     CDS             19452..20825
                     /locus_tag="U4A13_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1779527.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="U4A13_001218"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="U4A13_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN1779528.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="U4A13_001219"
     CDS             21880..22320
                     /locus_tag="U4A13_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1779529.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="U4A13_001220"
     CDS             22464..23165
                     /locus_tag="U4A13_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN1779530.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="U4A13_001221"
     CDS             23162..24283
                     /locus_tag="U4A13_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN1779531.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="U4A13_001222"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="U4A13_001222"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN1779532.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="U4A13_001223"
     CDS             25662..26570
                     /locus_tag="U4A13_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN1779533.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="U4A13_001224"
     CDS             26605..26877
                     /locus_tag="U4A13_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN1779534.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="U4A13_001225"
     CDS             26888..27934
                     /locus_tag="U4A13_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN1779535.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         9427 a         4850 c         6108 g         7679 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcg
//
LOCUS       DASJPC010000029        27526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJPC010000029 DASJPC010000000
VERSION     DASJPC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.029"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(407..766)
                     /locus_tag="U4A13_001226"
     CDS             complement(407..766)
                     /locus_tag="U4A13_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN1779536.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="U4A13_001227"
     CDS             complement(796..1137)
                     /locus_tag="U4A13_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779537.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="U4A13_001228"
     CDS             complement(1134..1808)
                     /locus_tag="U4A13_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1779538.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="U4A13_001229"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="U4A13_001229"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN1779539.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="U4A13_001230"
     CDS             complement(4322..5512)
                     /locus_tag="U4A13_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1779540.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="U4A13_001231"
     CDS             complement(5533..6060)
                     /locus_tag="U4A13_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN1779541.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="U4A13_001232"
     CDS             complement(6107..8896)
                     /locus_tag="U4A13_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1779542.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="U4A13_001233"
     CDS             complement(9043..9243)
                     /locus_tag="U4A13_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1779543.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="U4A13_001234"
     CDS             complement(9696..10016)
                     /locus_tag="U4A13_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779544.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10061..10204)
                     /locus_tag="U4A13_001235"
     CDS             complement(10061..10204)
                     /locus_tag="U4A13_001235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779545.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(10519..11637)
                     /locus_tag="U4A13_001236"
     CDS             complement(10519..11637)
                     /locus_tag="U4A13_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779546.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="U4A13_001237"
     CDS             12108..13415
                     /locus_tag="U4A13_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN1779547.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="U4A13_001238"
     CDS             13536..14735
                     /locus_tag="U4A13_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN1779548.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="U4A13_001239"
     CDS             complement(14762..16030)
                     /locus_tag="U4A13_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN1779549.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="U4A13_001240"
     CDS             complement(16200..16841)
                     /locus_tag="U4A13_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEN1779550.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="U4A13_001241"
     CDS             complement(17250..17753)
                     /locus_tag="U4A13_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1779551.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="U4A13_001242"
     CDS             complement(17876..18640)
                     /locus_tag="U4A13_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN1779552.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="U4A13_001243"
     CDS             18748..19023
                     /locus_tag="U4A13_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN1779553.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="U4A13_001244"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="U4A13_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1779554.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="U4A13_001245"
     CDS             complement(20579..20980)
                     /locus_tag="U4A13_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1779555.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="U4A13_001246"
     CDS             complement(20994..21803)
                     /locus_tag="U4A13_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1779556.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="U4A13_001247"
     CDS             complement(21790..22680)
                     /locus_tag="U4A13_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1779557.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="U4A13_001248"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="U4A13_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="U4A13_001249"
     CDS             complement(23348..24289)
                     /locus_tag="U4A13_001249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEN1779558.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="U4A13_001250"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="U4A13_001250"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1779559.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="U4A13_001251"
     CDS             complement(25274..26470)
                     /locus_tag="U4A13_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1779560.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="U4A13_001252"
     CDS             complement(26488..27216)
                     /locus_tag="U4A13_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1779561.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
BASE COUNT         7990 a         5888 c         4777 g         8871 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agctta
//
LOCUS       DASJPC010000030        27109 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJPC010000030 DASJPC010000000
VERSION     DASJPC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.030"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..74
                     /locus_tag="U4A13_001253"
     tRNA            2..74
                     /locus_tag="U4A13_001253"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="U4A13_001254"
     tRNA            78..150
                     /locus_tag="U4A13_001254"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="U4A13_001255"
     tRNA            155..225
                     /locus_tag="U4A13_001255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="U4A13_001256"
     tRNA            238..311
                     /locus_tag="U4A13_001256"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="U4A13_001257"
     tRNA            323..394
                     /locus_tag="U4A13_001257"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="U4A13_001258"
     CDS             660..1853
                     /locus_tag="U4A13_001258"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN1779562.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="U4A13_001259"
     CDS             1983..3455
                     /locus_tag="U4A13_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1779563.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="U4A13_001260"
     CDS             3538..4326
                     /locus_tag="U4A13_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN1779564.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="U4A13_001261"
     CDS             complement(4393..5061)
                     /locus_tag="U4A13_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN1779565.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="U4A13_001262"
     CDS             5176..6888
                     /locus_tag="U4A13_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779566.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="U4A13_001263"
     CDS             6891..8660
                     /locus_tag="U4A13_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779567.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="U4A13_001264"
     CDS             complement(8865..9431)
                     /locus_tag="U4A13_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1779568.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="U4A13_001265"
     CDS             complement(9431..10387)
                     /locus_tag="U4A13_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN1779569.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="U4A13_001266"
     CDS             complement(10387..11043)
                     /locus_tag="U4A13_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN1779570.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="U4A13_001267"
     CDS             11413..13431
                     /locus_tag="U4A13_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1779571.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /locus_tag="U4A13_001268"
     CDS             13858..16272
                     /locus_tag="U4A13_001268"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN1779572.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="U4A13_001269"
     CDS             16620..18173
                     /locus_tag="U4A13_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN1779573.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="U4A13_001270"
     CDS             complement(18554..20338)
                     /locus_tag="U4A13_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779574.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="U4A13_001271"
     CDS             complement(20353..22074)
                     /locus_tag="U4A13_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779575.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="U4A13_001272"
     CDS             22174..22935
                     /locus_tag="U4A13_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1779576.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23902..24170
                     /locus_tag="U4A13_001273"
                     /pseudo
     CDS             23902..24170
                     /locus_tag="U4A13_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24842..>27109
                     /locus_tag="U4A13_001274"
     CDS             24842..>27109
                     /locus_tag="U4A13_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779577.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAID"
BASE COUNT         8662 a         4770 c         5664 g         8013 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttttt gcttataaaa taattataag tatttagaga tttttcttct
    23461 aacattcttc aaacaagaaa aatcacaaag gttattgtta tttttatata ttctttttaa
    23521 gaatgattgt ttttttatag aaattttaaa aaataaaaca cgttattgat ggatagttag
    23581 tttgttattt ggtaatatta tagaagttga ctggtagtga tttatttttc gtcgttttat
    23641 aaaagtgtta ttttttggaa aaattcacaa atatgtaacg aatgtttttc tggaatttgc
    23701 tgtgttaaaa agcagtatga ctaatataaa tactattgtc atttatgcaa ttttttatgg
    23761 atagtaattt tagaggggaa tcaatgaagg aagaatagaa gggaaaaata gttgcaaata
    23821 cgccagttag caaaaatagc aatacatctt caagctttaa aattcgagag aatcatcttg
    23881 gtagtggaat caactacaac aaaaggatta aaccaattaa tagatagaat taccaatgga
    23941 aaaatagaaa aggtagttat tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt
    24001 agtgaaaccc tttgcaatat atggtacaga atatagaaat tattgacatt acagaaaatg
    24061 atgaagattt aatacagatt gtcactattt gtagatacct tttacaagga aaccgagcca
    24121 ataaagctaa gaagatgatt aaggagttga tggaagacga tacaagttaa gaaagtgaaa
    24181 ctacgcccaa ataaagagca aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca
    24241 tcgattcggt ggacagttta ttagtaatga cggtttgaga acatccatga cgagaatgaa
    24301 ggaacatcct aaatgcgttt agttgaacca agtgccaaat aatatggcca aataagcgat
    24361 cagttatcga tgaatcccaa gtattctaaa ctatatattc attttgatgc taagcattga
    24421 tatatttttt tgggattgat gaaggttaac caatacaggg attcactgat gaaattctag
    24481 agtctgacct tggtatcaac gatttagtga ttgtttctaa tggaaaagta taaaagtata
    24541 taagtacatt aacaaaataa ctattagggt tacgctccag cttgtgcaag gcactggcag
    24601 gaggaatcgt gaaatcataa ttatggttaa gccacacgga caactaaaag tcatacaaag
    24661 ctatcgaatc aatcaacaga agacaatcgg caatcattag ctttgtttac agcttacatt
    24721 gcaacgcttg agttgaaccg aaaatacgag ggaggtaacg cagccatttt gagttgggga
    24781 atctttttag aaaattatcg ggtaccttgt tgtttagtca aaattataag ggggagtaat
    24841 agtgaggaac aaatttcata aactgttaag tatgtttcta gttatgctta ttgtgatcaa
    24901 tcaatttggg aacataacgg ttctagctga gtctttagca gaacagccag agcgttcgct
    24961 gacgatcgaa gttgatcaag aggaggctgc tttgggtgag cagttccagt tgcgagtcag
    25021 aggaacacca gacctgctaa atgcattggt cattgataag agtgaggatt tgattgaagg
    25081 ggaagtgaag caactcaatg attacgaacg tctgattact tattcttccc agcattcagg
    25141 taaatacgaa ttgaccgcaa gaagcggtga tattcaatca aatacggtct caatagaaat
    25201 cagtgtacca gaagaaagca caagtgagtc ttttaatgta ccagaagaaa gcacaagtga
    25261 atcttttaat ataccagaag aaagcttaag tgaatctttt aatgaagcca gatcatctga
    25321 gccatctata acgcaggaat ctcagattga aagacaagag gagctcttga attatgacat
    25381 cggcatctct gctgagcgcc ccactttgcc gagcaggcat acacaaacgt taaagggata
    25441 cattcaagga ggcgctggcg atattcttga agaatatggg gagatggtcg tacgagttcc
    25501 tagagcatcg attggcttaa ttgagttcat tagagtggag gtcgctgatg attcaccttt
    25561 aaagccactg gcattttacc aagaaggcac agacgttgtt tttccattta cctttgatta
    25621 tgaaggatta aatgaagccg ctcagtcgta tacattcagt atcacgtttg aagttgcttt
    25681 ctttcaatca ggcggtactg agccaaataa tgactttcaa gtgagtctta cttatgctga
    25741 gcgagcagca aacacggaat tttcaattac gcctattcct tcatcaagac ccattttttc
    25801 gaaattatac tatggctcta gagaaatcga cagccaagga aatcttggga atctgctgat
    25861 tgacgggcaa gacgtgacca acagtaataa atttattttg caggtaaatt atgaaggatc
    25921 acgaaaagac ggtgtagtgg ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa
    25981 tccgactaca tggagttcca ctggaaatag agaacctatt tcaggtgggg ttcggatcgc
    26041 agaagtaacg aatccaggtg aagcaaacgt tagaactcat tatgtttccg aggaatttgt
    26101 ggacaaagta tcttttaata aggaaacgaa tacacttgtt gtggactttg gtaatctagc
    26161 agagggtcaa tcgtatttta ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa
    26221 tgaaggaaga caagaggatg ggttttctcg gaatcgagcg agtatgacct acaataatca
    26281 ggtaatgagc cgagactacc gcttgagaat ccctgaaagt aatcttccgg caatctcttt
    26341 aagaaaaagt gtggacaaaa atgttatcaa tctcaatgaa aatgaactaa cgtatactct
    26401 gcatgtctcg atgttttctg gaacaagaaa tacgcttgtt attgaagatg ttttggaaga
    26461 aggcatggaa tttgtgtcat ttctatcaga tattccctct ttcattgatg ccgaagtcaa
    26521 taatggaata gttcgtttca gtgcgaataa tacgattggt ggtccgacaa gttttaccct
    26581 ctcttttaag gtcaatgtag aggcgtatgc ggtcggcgat caaattttaa actttgcaac
    26641 gcttgtgaat agtgacaatc gattgaatac gagtactgta agaacaagga aaatcgatgg
    26701 gcgtattaaa gtaattaaaa ctgatgagaa tggccaaagg ctggcaggag caaaatttga
    26761 aattttatca agcgattcac aagtgataca agaaggtata acggcagaaa acggagaatt
    26821 tttaagtgaa ccgcttcctg taggtagcta tcaagtccga gaaacagaag caccggaagg
    26881 gtatctttta gatgcgacgg tacatgatgt ttttataaat gaatactcca tagaagaagt
    26941 gacgttaacc attgaaaaca tccttgatac gggtggtgtt gaactaatta aacatgctga
    27001 aggcagtggt gaagtccttc aaggagctgt ttttgagtta cagaaccgag aaggagaaac
    27061 gctacaaacc ggtttaacta caggagaaga tggaaaactg gcaattgac
//
LOCUS       DASJPC010000031        26605 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJPC010000031 DASJPC010000000
VERSION     DASJPC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.031"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1152
                     /locus_tag="U4A13_001275"
     CDS             <1..1152
                     /locus_tag="U4A13_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEN1779578.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="U4A13_001276"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="U4A13_001276"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN1779579.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="U4A13_001277"
     CDS             2728..3003
                     /locus_tag="U4A13_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN1779580.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="U4A13_001278"
     CDS             3009..3776
                     /locus_tag="U4A13_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN1779581.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="U4A13_001279"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="U4A13_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN1779582.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="U4A13_001280"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="U4A13_001280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN1779583.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="U4A13_001281"
     CDS             7920..8222
                     /locus_tag="U4A13_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN1779584.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="U4A13_001282"
     CDS             8225..9388
                     /locus_tag="U4A13_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1779585.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="U4A13_001283"
     CDS             9414..12842
                     /locus_tag="U4A13_001283"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN1779586.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="U4A13_001284"
     CDS             12863..13987
                     /locus_tag="U4A13_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779587.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="U4A13_001285"
     CDS             13977..14414
                     /locus_tag="U4A13_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1779588.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="U4A13_001286"
     CDS             14398..14736
                     /locus_tag="U4A13_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN1779589.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="U4A13_001287"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="U4A13_001287"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN1779590.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="U4A13_001288"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="U4A13_001288"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN1779591.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="U4A13_001289"
     CDS             15893..16936
                     /locus_tag="U4A13_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN1779592.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="U4A13_001290"
     CDS             17095..17823
                     /locus_tag="U4A13_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN1779593.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="U4A13_001291"
     CDS             17939..18184
                     /locus_tag="U4A13_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1779594.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="U4A13_001292"
     CDS             18181..18399
                     /locus_tag="U4A13_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN1779595.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="U4A13_001293"
     CDS             18535..19074
                     /locus_tag="U4A13_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN1779596.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="U4A13_001294"
     CDS             19067..21340
                     /locus_tag="U4A13_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN1779597.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="U4A13_001295"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="U4A13_001295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN1779598.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="U4A13_001296"
     CDS             complement(22513..22767)
                     /locus_tag="U4A13_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN1779599.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="U4A13_001297"
     CDS             complement(23167..23436)
                     /locus_tag="U4A13_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779600.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="U4A13_001298"
     CDS             complement(23906..24361)
                     /locus_tag="U4A13_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN1779601.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="U4A13_001299"
     CDS             complement(24499..26427)
                     /locus_tag="U4A13_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN1779602.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8861 a         4610 c         5614 g         7520 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taaga
//
LOCUS       DASJPC010000032        25184 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJPC010000032 DASJPC010000000
VERSION     DASJPC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.032"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..746
                     /locus_tag="U4A13_001300"
     CDS             99..746
                     /locus_tag="U4A13_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN1779603.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="U4A13_001301"
     CDS             1139..3784
                     /locus_tag="U4A13_001301"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN1779604.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="U4A13_001302"
     CDS             4094..5407
                     /locus_tag="U4A13_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN1779605.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="U4A13_001303"
     CDS             5397..6050
                     /locus_tag="U4A13_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1779606.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="U4A13_001304"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="U4A13_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="U4A13_001305"
     CDS             6787..7872
                     /locus_tag="U4A13_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1779607.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="U4A13_001306"
     CDS             7869..9761
                     /locus_tag="U4A13_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1779608.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="U4A13_001307"
     CDS             9768..10145
                     /locus_tag="U4A13_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1779609.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="U4A13_001308"
     CDS             10299..11081
                     /locus_tag="U4A13_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEN1779610.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="U4A13_001309"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="U4A13_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEN1779611.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="U4A13_001310"
     CDS             complement(11996..12055)
                     /locus_tag="U4A13_001310"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEN1779612.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="U4A13_001311"
     CDS             12447..13109
                     /locus_tag="U4A13_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1779613.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="U4A13_001312"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="U4A13_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="U4A13_001313"
     CDS             13637..13837
                     /locus_tag="U4A13_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1779614.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="U4A13_001314"
     CDS             complement(14091..15770)
                     /locus_tag="U4A13_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1779615.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="U4A13_001315"
     CDS             complement(15772..15984)
                     /locus_tag="U4A13_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN1779616.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="U4A13_001316"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="U4A13_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN1779617.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="U4A13_001317"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="U4A13_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN1779618.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="U4A13_001318"
     CDS             19959..20156
                     /locus_tag="U4A13_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779619.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="U4A13_001319"
     CDS             20309..20794
                     /locus_tag="U4A13_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN1779620.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="U4A13_001320"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="U4A13_001320"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN1779621.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="U4A13_001321"
     CDS             21671..22198
                     /locus_tag="U4A13_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779622.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="U4A13_001322"
     CDS             22289..23026
                     /locus_tag="U4A13_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1779623.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="U4A13_001323"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="U4A13_001323"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN1779624.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="U4A13_001324"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="U4A13_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN1779625.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8387 a         4103 c         5297 g         7397 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa tttt
//
LOCUS       DASJPC010000033        24762 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJPC010000033 DASJPC010000000
VERSION     DASJPC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.033"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(233..1015)
                     /locus_tag="U4A13_001325"
     CDS             complement(233..1015)
                     /locus_tag="U4A13_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779626.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1080..1916)
                     /locus_tag="U4A13_001326"
     CDS             complement(1080..1916)
                     /locus_tag="U4A13_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1779627.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="U4A13_001327"
     CDS             complement(1934..3343)
                     /locus_tag="U4A13_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1779628.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="U4A13_001328"
     CDS             complement(3357..5180)
                     /locus_tag="U4A13_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1779629.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5460..5687)
                     /locus_tag="U4A13_001329"
     CDS             complement(5460..5687)
                     /locus_tag="U4A13_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779630.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
     gene            complement(6023..6349)
                     /locus_tag="U4A13_001330"
     CDS             complement(6023..6349)
                     /locus_tag="U4A13_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779631.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6395..6757)
                     /locus_tag="U4A13_001331"
     CDS             complement(6395..6757)
                     /locus_tag="U4A13_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEN1779632.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6794..7009)
                     /locus_tag="U4A13_001332"
     CDS             complement(6794..7009)
                     /locus_tag="U4A13_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779633.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7136..7471
                     /locus_tag="U4A13_001333"
     CDS             7136..7471
                     /locus_tag="U4A13_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779634.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7547..9544)
                     /locus_tag="U4A13_001334"
     CDS             complement(7547..9544)
                     /locus_tag="U4A13_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779635.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9642..9917)
                     /locus_tag="U4A13_001335"
     CDS             complement(9642..9917)
                     /locus_tag="U4A13_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779636.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10149..10883
                     /locus_tag="U4A13_001336"
     CDS             10149..10883
                     /locus_tag="U4A13_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779637.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            10922..12064
                     /locus_tag="U4A13_001337"
     CDS             10922..12064
                     /locus_tag="U4A13_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1779638.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12159..13550)
                     /gene="sufB"
                     /locus_tag="U4A13_001338"
     CDS             complement(12159..13550)
                     /gene="sufB"
                     /locus_tag="U4A13_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN1779639.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13555..14025)
                     /locus_tag="U4A13_001339"
     CDS             complement(13555..14025)
                     /locus_tag="U4A13_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN1779640.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14012..15247)
                     /locus_tag="U4A13_001340"
     CDS             complement(14012..15247)
                     /locus_tag="U4A13_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1779641.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15244..16530)
                     /gene="sufD"
                     /locus_tag="U4A13_001341"
     CDS             complement(15244..16530)
                     /gene="sufD"
                     /locus_tag="U4A13_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN1779642.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16543..17316)
                     /gene="sufC"
                     /locus_tag="U4A13_001342"
     CDS             complement(16543..17316)
                     /gene="sufC"
                     /locus_tag="U4A13_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN1779643.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17513..18361)
                     /locus_tag="U4A13_001343"
     CDS             complement(17513..18361)
                     /locus_tag="U4A13_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN1779644.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18385..19071)
                     /locus_tag="U4A13_001344"
     CDS             complement(18385..19071)
                     /locus_tag="U4A13_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779645.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19064..20098)
                     /locus_tag="U4A13_001345"
     CDS             complement(19064..20098)
                     /locus_tag="U4A13_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1779646.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20161..20417)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20493..20831)
                     /locus_tag="U4A13_001346"
     CDS             complement(20493..20831)
                     /locus_tag="U4A13_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN1779647.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20833..21189)
                     /locus_tag="U4A13_001347"
     CDS             complement(20833..21189)
                     /locus_tag="U4A13_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN1779648.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21195..22382)
                     /locus_tag="U4A13_001348"
     CDS             complement(21195..22382)
                     /locus_tag="U4A13_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN1779649.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22573..23418)
                     /locus_tag="U4A13_001349"
     CDS             complement(22573..23418)
                     /locus_tag="U4A13_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1779650.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23652..24617
                     /locus_tag="U4A13_001350"
     CDS             23652..24617
                     /locus_tag="U4A13_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN1779651.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7220 a         5217 c         4217 g         8108 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcgata gaatgatttg ggctagtatt cttaaaaatt aactagcaaa tgtttaaact
     5761 gatttatttc taatctacat gtagtaagta tttcaattcc accaattatc aataaaaata
     5821 ccaaacatat ccataataat tttttctttt tcatttcttt tatattgtta taacagtgaa
     5881 taacataaaa attcccgaca atcattgtaa tatttttttg tcacctgtat tagtatttaa
     5941 tttataggaa actataaaaa ctccctagtg aatttacacc tttgggagtt cctgtttgga
     6001 cggtatgcat aatacattta aatcaattta acattaacac ctcagcatat ggtatctcca
     6061 catatatacg gtctttcttt ggatagtact caatatttat gttgatagca cttccattgt
     6121 ctaatatagc tttagcccgg tatgtgttat ctccattttc atgagtaatc gtcacatcat
     6181 cacatgatcg atttaagttg tactgttctt gtaaaatctg tgttaccaat ggttttgatg
     6241 acttctctaa tccagatgta cctgtcattt ttgaaaaaat aatacctata atagccaaga
     6301 caacaatacc tataatgaca aacttcttgt tcatatttac tttattcaac tttttatctc
     6361 ctatctccaa aataatttgc tcttatccct cttatcagta cattgacatc cctaaaagta
     6421 caccaaagat tgctccagct acccctgcta tcatcaaaat cgtacccatt ttctcatctt
     6481 tttctcgata taagtagccc ataataacag caacagcacc aaatacaaca ggaataaatg
     6541 ctaacgatac taccatagaa atccaaccaa taatcaaata cagcttccct gtctcttgtc
     6601 catgtacgat ttgttccttc atttcattaa ttgtctgctc agtacttttc tttactgtgt
     6661 ctgttccggt tgtctcatct gtttcttgta gtgctccaca aaactggcaa tacttacttt
     6721 catctgttat ttctttgcca cattttctac aatacatgtc ttctcctcct aaaatcattc
     6781 aattgcttat tagttaactg tttactttat taaatacatc agaaaacagc tcattcattg
     6841 gtacacttaa tatctcagaa agcatcttca tttgataggg tttaggaatc gttctccctg
     6901 tttgccacga acttacacat tgctgtgtaa cattcatttt ttttgctaac tccgtttgag
     6961 aaagtccttt ttcttttctg tatcggctga gattacttac ttttttcatg attctcccct
     7021 cctgttttat tatatacaac aatatgatgt atttcaactc ataaaatata aaaaacttca
     7081 aataccatta caaatacaac aacttgttgt actatactga gatgggggtg tattaatgaa
     7141 acagaaacaa tatgcaaagg tcattagaga aaagcgaaaa gctctcgggt ggacacaaaa
     7201 agaattagct gaaaaaatct tctctacaca acaggcagta gcaagatggg aaaattctgt
     7261 aacagagcct aacctcgata gcttaacagc tctttctaga gccctaggaa cccctgtaag
     7321 ccattttcta gacaatgttg tcgtagatta tgaggaagag tttttggctc tctatcgttc
     7381 tcttagcact gaagatgcag ttcgaacaat tgactatatg aagctattaa aaaggcaaga
     7441 aaatgaacgc aatcagctat tgaaagattg aaaaataaat aacccaccga actttaacgg
     7501 tgggtgtatt gagatgaaat aagattaaga gctatggata acctgtctag tttaatacaa
     7561 ttgggtcttt tctcttcctt actctccgtc catcatctct tgttacactt gtaaattgat
     7621 acagaggaac tatcccagct cctgataata tcaattcttt tattgtttca tctatccctg
     7681 cccctttcgc taaatatatt aaaaaactac tacttgcttt cacattgtat agtttcttaa
     7741 tatccttaga agctcgttca agagtgaatc cttgtttaac aaagtagcct ctgtttttta
     7801 ttaaagcatt tttcacgctg ttatccccaa cattacgtag gcgttcacaa acagtgttaa
     7861 taagttcttt ctctgtcatt agatatgtcc cttgccacga taagccctgt aaaacttcat
     7921 gtgcttcatc taaaatcaat tgtttttcct tgctgagttc tgatcgggaa ttttgaggaa
     7981 atactctttt agcctctttt tcgttaaaac atatcttaac ccattcataa gaaaaatatt
     8041 gatatgtaaa atgaattttt tccagaagtg gaaggatatc ttcattcaat ttttgaatca
     8101 cagaagttgc attaaaaacg tctaatcgat agacattgct tctcttatga ttaaagtttc
     8161 ctaacgcttt ctcttccttt aacctattta ggtattcgag catcttcaaa ggaacatctt
     8221 catcatctaa tttttttatc aatcctataa aagtcataag tgatagtaac ttattacatt
     8281 tatctatgtc ataagtttta ccatagtaag aactcatttt tttagctaat tgttcccccg
     8341 acaaaataca agtcacactt tccttactat catcaaaaaa attaacatca gaaagcaaga
     8401 catcatagac acgtcgcatt tcctctagat aattccttat tgtcctgaaa agtttgtctt
     8461 tcattgattc aagagtgtca aaaagtactt ttgattcctc tttcaatttt cgaaaatgtg
     8521 taggatacaa gtctaggttc tctaatagcc aattaaatgt ttctacccga ctgtctttct
     8581 ttaaaattag catcacaact ccaataaaat tttcactgat atcacaatta ctggaatgac
     8641 aataaaaaat ggaatgccca gttttactgg taaaaatact agcagaggga ttatgatcgt
     8701 tgtgaaacag acatcttaag tttttacttg acttaagtaa tttattcata tcaatggtta
     8761 acaatctttc ataaatctca ttaaaagaca tgtcggagga atcaaacagg cagtctttaa
     8821 accactgttt catttcttct ttgtcgtcat tttttatcaa cttaacaaaa tcggtttcat
     8881 tgactagttc atactctctc cctgaatcac tttgacaatt tttagaacat gtcatggtaa
     8941 aatcaatcgg aagctcagta atatctaaca tgttgtcttc attaattatt atcactgcat
     9001 ctgacttata tcctccaaaa aacattctat tcgaagcatt tacattgtca tcacacccgg
     9061 ggatgttctt tttcaagtat tcgtaaacag caaccatttt accatagtca gtaattgctt
     9121 cttttaatag aaagacaatc ttgtattttt ctagattttc agagtaccga atactttttt
     9181 gtatcgcaaa cgcagaatta tgaactaaat tgatagataa aaccttttct acgcttaagt
     9241 atctactatc atctttactc aaatctacta ccttcccctt ctcatcttta acagtgttat
     9301 cgacatccag aataattact ctggtttcca caaaactctc cttgtcattg cctttattct
     9361 ttgtcttgct tggaataaat gattgtgatg aatttagtat ctctacaaac tcatctcttg
     9421 taacttctct atagttatct aaacacctat ctcttacatc agttttttct ttttttgtaa
     9481 ctggcttctc taataatttt tcttgatcta cataaattgg aatattaatc attgatgttt
     9541 ccatttgtgt ttcctcctta taaaattctg ttaaagacta aaacatacag ataataaaaa
     9601 caaactcttt attactatat gttttagtct tatttgttta ctcaaccttt ataccgttcc
     9661 atgaaactat caatatcaga ttttttaaat ctaatgaccg tatcaatttt tataacaggt
     9721 aacccgtatt gagaaacata ttttctaatc gttcctactg atacacctag atattcactt
     9781 gcctgctgat agtccatata ctctagagtg actccagaaa taatgtcttc tagcataggc
     9841 tttagcactg taattatctc tttcacttgt tgttcaactt gtatagaaac aatgctatca
     9901 atgatttgta gagacatatt tccccctcct ttcgtgattg cttgaacaaa ttttctaaga
     9961 attatcttcg tattttttgc tcaaaataaa agtattttgt tattaaccga ttaatcaaac
    10021 aaaaaatgat taccagttca tcgacataca cataatacct cgataaaaaa ggggtgtcaa
    10081 cagctttgta aaatttattt gcaacacccc ttttttgagg tatactaatt ttaggaggtg
    10141 tggtcaatat gataaaacgt ccaccaataa attatttaga acgaaaaaag atactcggaa
    10201 caaaaataaa agctataaga aagagtaaaa aattaacaca accagcattt ggtcttatga
    10261 ttaacaacgg tcaattaatt gacaaaaaaa ctatctacga atgggagaag ggaacctatt
    10321 taccaatacc agagcgatta tcccgaattg ctgatttggg aaatatgagt atagaggagt
    10381 tagtttgtgg caatgtagaa gaatatattt tgggaattat tctttataga gatagcattg
    10441 tactagatgg tataacattt ccagataaaa acctgttcca gcatcttcgt caacagttcc
    10501 caccagtaca ctctaattta gatacatggc tggatagata ttccaaatta gaaccagaga
    10561 tgcaagaatt tattgctaac aagacttgta ataaagttaa aaatgaaaaa attagtcttt
    10621 tcaatatctt aaaaatagaa gaattattca tcaatgctat tgtagaagaa tttgacaata
    10681 acattctttt cctcactagc agtatagaag aactactaga aagaatggta gatgaatggc
    10741 tacctattca attgaaagac atgtcttatc ccgaagaagc tgtgagagaa attactgata
    10801 atataaacaa gctagaacaa acgatttcat caattgggaa aaaatacact aagaaaaaaa
    10861 tgaagggagg tgataccatc tagtggtaag tactccttgt aaactaagag gagatgattt
    10921 aatggcaaaa atatcaaagt acaaaaagaa aaacggtcaa gtggcgtgga tgtataaagg
    10981 acacattgcc accgatccac gaactggtga aaaaataaat actacgagaa gaggttttag
    11041 tagcaaacaa gaagctcaaa aagattatga cgagtacaga catcaaatat tatacggtgt
    11101 taaaaagaaa gcacctgata tgacatttga agacctgtat aatgaatgga taactcatca
    11161 gcgtaccagt gttaaagctt caacaatcgc aatctcagta agatacgcaa ataatcaaat
    11221 acttccagca tttggtaagc ttaaactatc gaatatctct gtcccatatt gtcaaaaagt
    11281 ggtagatgaa tggcacagta aatatgaaag ttacgattat atgagaaaac agactgcaca
    11341 aattttacgc tacggggttg caatgcaata tattgacaat aatcctatgg aaaaaacact
    11401 tcttccacga aaaaaagagt atgagaaaaa tcgtaaattt tactcaaaag aggagcttaa
    11461 taatttactg gatgctttta aagattttgg taacatgaaa caatacgcct tcttcagatt
    11521 attggcatac acaggtatga gaaagagtga agtactcgct ttacagtgga aagacataga
    11581 cacgtttaat aaagaactac atgtcaataa aactcttgct gtagatgaat ttggtaaagt
    11641 tataattcaa tctccgaaaa caagagcttc tagacgagtt atctctttag atactgagac
    11701 tctttctatc ctcaacaact ggaaattaca gcaaaaagaa gagtatttaa aacttggata
    11761 caatactagc tctaaagagc aacatgtttt tacaacagtt aaaaacactc tatacattcc
    11821 aaacactgtt aatgattggt taagatatat tttgaaaaaa tataacttgc ctcgtattac
    11881 acctcatggg tttagacata cacatgctag tttattatta gaagcaggag aatctgtcaa
    11941 ggtagttcaa caacgattag ggcatgaaaa tagtaaagtg acattagaca tctatgcaca
    12001 tattacaaat aatgcaccta agaaaacagg tcaagacttt gctgatatga tggctcatca
    12061 gtaaaagagc tggtcaaaat ctggtcattt ctagtataca ggcaaaacaa aaaagctaga
    12121 acccttatat atcaagggtt ctagctccta taacaggttt acccaacact gccttccatc
    12181 tcataactga tcaatcgatt gagttcgact gcatattcca ttggaagttc tttggtgaat
    12241 ggttcaacaa agcccatgac gatcatttca gttgcttcag attcggtcag gcctcggctc
    12301 atcagatagt agagttgttc ttctgagatt tttgagactt tggcttcgtg ttctaaagag
    12361 acttggctgt tgtggatctc attgaatggg atcgtatcgg atttcgacca ttcgtccatg
    12421 atgatcgtgt cacattcgat atgagagatc gaacccgcac tgtttttggc aaaggttact
    12481 tgtcctcgat agtttacttc tccgccgtct tttgaaattg atttagagac gatagagcta
    12541 gatgtatttg gtgcattgtg gatcattttt gcacccgtat cttggatttg gtttgctcct
    12601 gcaaaggcga tggagagcat ggtgcctcgt gcaccttttc catctaagta aacacttggg
    12661 tatttcatcg ttgtttttgc acctaggttt ccgtcgatcc attcaacggt tgcgccttcg
    12721 tatgcttttg ctcgtttggt tactaagttg tacacattgt ctgaccagtt ttggatcgtt
    12781 gtgtaacggc aatacgcatc ttttcttgta aagatttcta cgatggcggc atgcaggctg
    12841 ttacttgaat aagtaggtgc cgtacaacct tcgacatagt ggatgcttgc tccttcgtca
    12901 acaatgatca gcgtacgttc gaattgcccc gtgttttccg cattgatccg gaagtacgtt
    12961 tgtaatggaa catcgacttt gacgcctttt ggcacgtaaa taaaggtccc acctgaccat
    13021 acggctgaat tcagcgctgc taatttgttg tcggttggcg gtacaagttt tgaaaagtac
    13081 tctttgaata attcagggta ttctttcaaa gcagagtcag tgtcagtaaa gacgattcct
    13141 aacttttcga attcatcttt catattatgg tacacgactt ctgattcata ttgtgcggaa
    13201 gcaccggcta gataagctcg ttcagcttct ggtataccaa tcttttcaaa tgtatctttg
    13261 attttttctg gtacgtcttc ccagtcacga gcgggtttgt cacttggttt ttggtaatat
    13321 ttgatggcat cgaaatcgat gtctgaaagg tcggggcccc atgtttgcat tggcattttg
    13381 ttaaatgttt ctagagattt cagacggaat tcaagcatcc attctggttc gcctttgatt
    13441 cttgacatct cacggatcac ttcttctgtc aagccttctc ctgtactgaa aaccggttcg
    13501 acatcatcgt gaaacccgaa tttatattct tctaattctg gtacgcccat gttctcactc
    13561 ctcttccttg tggtaatgat ggaaagctgc ggtaccatcg ttttccattg cttttcctag
    13621 tgctttccag gcaagtgtcg cacatttgat ccgagcggga aatttagcta caccaccaag
    13681 catagcagca tcgcctaatt ggtcgggatc tttgacttct tttccttgga caagctgtag
    13741 aaagtctaca gttaaatgtt ctgcttcttc tattgatttt ccgatgacgg cgtctgtcat
    13801 catcgaggca cttgccgtac tgatggagca gccactgccg tcaaaggcga tatttttgat
    13861 gattccgtct tttacatcga cttgcaattc gatcacatct ccacatgtgg ggttgttcaa
    13921 ttcgatcttg cttgtcgagt cagtcaagct tccacgatga tgcggatggg aagaatgatc
    13981 caagatcact tgacggtata aattgtctaa tttagatagt cccatgttgg aaaaactcct
    14041 ttgttgccat gattgcttca actaaacggt cggcgtcttg cgttgtgttg tagaaataaa
    14101 agctcgcgcg agcagtcgct gatacttcaa ggtacttcaa tagcggttgc gcacaatgat
    14161 gacctgctct gacggctacc ccttccatgt ctaaagctgt cgcgacatca tggggatgta
    14221 acccatccaa attgaatgca acgactcctg tccgaaaatt cgggtcttgt ggaccataaa
    14281 cagtcaatcc atcaattgct tgtaattttg gcaacacata agcggctagt tcttcttcat
    14341 atcgatggat attgtccata ccgagttttt gcaaataatc gattgcatgg cctaatgcga
    14401 tagcgccggc gatatttggt gtccctgctt cgaatttcca aggtagttct ttccatgtac
    14461 tgtcgtataa ttcgacaaaa tcgatcattt cgccaccaaa ttcaacaggt tccatttctt
    14521 ctaaccattt tcgtttacca tataaaacac caatacctgt tggtccgcac attttgtgtc
    14581 cgctgaaagc aaagaaatca cagtctagtg cttggacatc aatggccatg tgcggggcag
    14641 attgagcacc atctactacg atgaccccgc cgttttcatg gacaagttca gctaattctt
    14701 gaacaggatt gaagacacct aagacattgg atacatgagc aatggagacg attttggttt
    14761 tgtccgtaat ctttttcgct gcatcctcca tatccaggaa gccttcttct gttagttcga
    14821 tatatttcaa gacagcacct gttcgttttg ccaactgctg ccacgggata acattagaat
    14881 gatgttccat gtaagagagc actatctcgt ctcctgcttt gatatagcgg tcgccgaagg
    14941 ttctggctac ccaattcaag cttgtcgtcg taccacgagt aaacagcact tctgctgttt
    15001 cttttgcatg gatgaacgcc cgtacttttt ctcttgcttg ttcataatca tgggtcgcgc
    15061 gttctgctaa cgtatggaca ccgcgatgaa cattggcgtt atcgtgttcg taatagtacg
    15121 ctaatgcctg gagtacttgt tttggctttt gggtcgtcgc tgcattatcc agataaacta
    15181 aaggttcatc attgacgtct tgaaataaga taggaaaatc attgcgtagc tttttagcat
    15241 cgatcatcca ttcaacttcc cttcgattac ttcgactaat tctttccgaa cttgttctac
    15301 agggattgct gttaagacag aaccgaggaa tcctcgaatc actaagcgct ctgcttcttc
    15361 tttatgcaat cctctactca tcagatagta catttcttct ggatcaactc gtcctacact
    15421 ggcagcatgt ccagcagtta cttcgttttc atcgatcaaa agaataggat tcgcatcacc
    15481 acgtgcttta tcagaaagca tcaagacacg actttcttgt tgggcatctg caccttttgc
    15541 ccctttcaag atgtggccga taccattaaa ggtcaatgtt cctttttctc ggatcactcc
    15601 gtgttgcaaa atatgcccga tcgtatgagg ggctttattg gttactcgtg tatcgattcc
    15661 ttggatttgt ctgcccgagc tgattgccac gattttgact tctgcgtgtg caccttctcc
    15721 tgctaaatct gagtcaaagt cagcgatcac atgaccgtca ttcatgacac caattgccca
    15781 gtcgacagaa gcatctcgta aaatgtgccc acggcgattc atataagaag taatattttc
    15841 tcctaattga tctacggctg aatatttgat ttttgaacca gctttagcga tgacttccac
    15901 gatgatatta cctgatgatt ttgctacttg ttctttggtt gtctggaaac gttccagata
    15961 agaaaattca ctatgttcat ctgccacgat caacacatgc ttgaagaagt gctcatcgga
    16021 tgcgccatct tgaatgaata aggactcgag tggttcatcg atcacgacat ttttaggcac
    16081 atacaggaaa ataccgctgt tcataaaagc tacatggagt gcagtcaatt ggtcttcgtt
    16141 catgtttact gcttttttca tataatattc ttggactagt tctggatatt cgatcattgc
    16201 agtgaacaag tcggtaaaaa tgactccttt ttctgctaat tccacaggca gttgttcaaa
    16261 aatcgtccat gaaccttgtt ggacaagcac aggattgtct ttcattgcat caaatgccgg
    16321 aatcgtgcct gtttcactga tggtttgtgt ttctttcaca tcaaataacg accagcgatg
    16381 gaatttcacc cgatcgatat gcggcaatgg cagttcatct gctttcgcta gtgcgtcttg
    16441 acggaaggtt ctcatccaat caggttcgcc tttacttaaa gaaaaatcat ttaccgcgtc
    16501 aagataatcg agccagtttt tttctttcat tcttgctccc ccttacgctt cttcttcttt
    16561 atagtcgatt cctaattctt tactgatacc agcgtagcct tctgcttcca gacgtttagc
    16621 taggtcagca ttccctgtca tgactactcg gccttccatc atgatgtgaa cgacatcagg
    16681 tgtgatatag ttcaataatc gttgatagtg ggtgatgatc aatgcaccga aattgtctcc
    16741 gcgcatttca tttactcctt tagcaacgac tttcaaagca tcgatatcca gacctgaatc
    16801 gatttcgtct aaaattgcaa atgtaggttc taacatcaac aattgcagga tctcattccg
    16861 tttcttttct ccgccagaaa aaccttcgtt cagataacgt tcagccattt cttctggcat
    16921 atttagtaaa gccatttttt catctaactt tttcaaaaat gccataacag aaattttgtc
    16981 gttttcatca cgtttcgcat tgatcgctgc acgcatgaat tccgcattcg tgatacctgg
    17041 tatctcactt ggatattgca tagctaaaaa taagccaagt cgcgcacgtt catctacttc
    17101 caagtctaaa acatttttcc cgtcaaacaa aatttcgcct tctgttactt catagtttgg
    17161 attgcccatg atagcggcag ataaggtaga ttttcctgtg ccgttaggtc ccatgatggc
    17221 atggatttct ccggttttca ttgttaggtt tacgccttta aggatttctt tttcctcgat
    17281 cgacacatgt agatttttta tttctaagac agacatgcat tataccccca aattctcttt
    17341 cgttctcact cattttagac taattgagaa tgaagcgcaa tgaccttcct tactaaaatc
    17401 aaatatcttt tatttattat taaacaattt tgatttctaa attagaatag gtataaaaaa
    17461 gatgagaaat aagccattac agacttattt ctcatctttt ttgttccata ccttattttg
    17521 catcagcctt tactggttta acagagccgc cccatttgtc tgtgatccat tcttggactt
    17581 ctttgcggtg aagcacttca atcagttttt tgattttgtc actgtcttca tcacctttac
    17641 gagtcgcaat aatattcacg taaggagagt tgtccttttc aagcaatacg gcatcttcta
    17701 atggattcaa ccctgcgccg taagcgaagt ttgcgttgat tgcaactagg tcaccttcat
    17761 cattttggta cgtagaagct aaaagtgatg ggtcgatcgt atggttgaac ttcaagttct
    17821 tagggttttc ttcaatatca tcgaatgttg ctgtttccag atctactcca tctttgactt
    17881 tgactaagcc agcatcttgt agaattgtga tgatgcgtcc ccagtctgat tcagaatttg
    17941 aagtaatgat tgttgcacca tcttttaatt cggaaacatc tttgactttt ttagagtaca
    18001 agcccattgg ttcgatgtgg acagcaccgg cattgacaaa gtcatatcct ttttctttga
    18061 tttctttgtt caaataagga atatgctgga agtaattggc atcaatgtcg ccttcttcca
    18121 aagattggtt cggtaataca tagtcatcga atttgacgat ttcaagatcg attccttcat
    18181 ctttcaataa tggtttcaca tgttccaaaa tctctgcgtg cggtgtcggt gaagcgccga
    18241 ctttcaatgt cgtgtcgtct gctttatttg ctgaatcttt gctgtcgctg ttgttaccgc
    18301 atgctgctaa tcctacagtc agcaaaagcg ctgctgcaaa tccaaaaatt ttctttttca
    18361 tatcctacat ccccctatat cctattatct tttgtctgtt ttcttagtta agaaatcacc
    18421 gattccttga acgataaata cgattagtaa aataattaaa gtcgcaacga atgtcactgt
    18481 tagattacca cgctggtagc cttcctgcca agcaagacct ccaagtccac ctgcgccgat
    18541 cgcgccggcc atcgcagtaa atccgatcat cgagatcgtt gtaactgtta atccagaaat
    18601 caatgcggga atactttcag gtatcaagac cttccagatg atttcccagt aacttgctcc
    18661 cattgcatct gctgcttcca gtacaccagg gctgacttca cgaaaagcaa tctctactag
    18721 acgggcataa aatggtgctg cggataaaac aagagctgga atggctgctt gcgcacctag
    18781 catagtgcct acgattgctt tagtaaatgg aatgatcaaa accatcagga tcataaatgg
    18841 cgttgaccgg aaaatattac tgatgatcga tacgattcca taaatgattc gagcatacgg
    18901 tttgtttttg cgtccaatag agtaaaggat caatcccaaa actaatccga tgatcgtcac
    18961 taagaccatt gagattaatg tcatatatag tgtatcaagg atagcctgat tcatcgtttc
    19021 cgggttgatt tgtttaaaat ctagatatgt ttctgaaaag cttttatcca cgatggatca
    19081 cctcaattcc tactccttgt tctttgatga acacttctgc ttgatctaat tctttggttt
    19141 caccagtcat caagacagtc aatgaaccaa aggaaccttc tttcacttgt ttgatctttc
    19201 catacacaac gttgacatcg atgttatagc gtcgaatcgc ttgggaaatg actggctcgt
    19261 tggcatttct ttcgctaaag gtcaaagttg ccaaacggcc gcttggattt tctttgatga
    19321 gttcagctaa tagttctgtc gaatcagctt ctggttcgat gtcttgttgt acaaagcgtt
    19381 tggttacttc ttttttcggt tgtctgaaga cagtcaatac gtctccttct tctacgactt
    19441 tccccaactc catcacagcg actttgttgc agattttgcg gatcacattc atttcatgtg
    19501 tgatcaagac gatggtcaaa ttcaatttct tattgatcgc taacaataag tctaatactt
    19561 cttctgttgt ttgcgggtcc aatgcgctag tagcctcatc acaaagcagg atctcaggat
    19621 catttgccaa tgctcgtgcg ataccgacgc gttgtttctg tccgccggaa agttgtgaag
    19681 gatatgcttg tccgcgtcct tctaacccga ctaaacgcaa cagttcttct gcccgttctt
    19741 tacgtttgct ctttggtacg ccagctaatt ccagcggcag ctggatattt tcaagtacag
    19801 ttcttgacca taacaggttg aaatgctgga agatcatacc gattttcttt cggaatgtac
    19861 gtaattcttt gttttttaat tttgtaatat cttgatcgtt tacgatcact tcgccgctcg
    19921 tgggaagctc aagtccattc aggagacgga ctaatgtact ttttcctgcg ccagaatagc
    19981 cgacgattcc ataaatatcc cctttttcaa cgtttagtga aacgtcattg acggcttgga
    20041 tgtttccgtt tttcccattg aatgtctttt tgacattcgt tagttggatt aatgacatgc
    20101 cattcacatc tttctcttat gatttttcaa acaaaaaagc cttcgccctc ttgccttaag
    20161 caaaaggacg aaagcttgaa cttccgtgtt accaccttga tttaccgtta cttcgcaata
    20221 acagcctctt ccagtacttg gacttagctt ctttccgtat actgtcgcgc tgtaacgggc
    20281 actcccgtag taagtttgct tacgctcgct tactctgctc taagaccatc ttccatctca
    20341 ttttcgttac ccattttcag caaacagggc tctcttcaaa cgtccgtgac atgtactctt
    20401 cttttcaaag ctttctcgtt tcatatagga ttgcttatga ttatagcaac tggaagaatc
    20461 agtgtcaact tttttcaagt gattttctga ttttataatt cgtcaaattc ttttggatca
    20521 acatctttga aacttaagat ccacgcattc atttcatctg aatcattcaa gatattgata
    20581 tcttcaccga ttttatcatt aacagaagaa actgtacccg ttagcgggct ggcaaattca
    20641 ttgaccgctt tttccgcttc aacttcgatc agaggttcat ctttttttag cgattgtcct
    20701 gcttttggca gattggcaaa cgtgatgtct ccaagttctt cttgcgcttc atttgtcaaa
    20761 ccaacacagt attcttttcc attaaataag atccaaaggt tgtccttttt cttcaaatgc
    20821 ttgcctgcca ttttatatca gttctccttc ataaattttt tcattgaatc caattgcaac
    20881 aagctggtcc tctcgtaaga tcagtggtcg cttgatcagc ataccatctg tcgataagac
    20941 ttggctcgct tttgaagccg taaactcgcc tacttgatct ttcagtccaa gttcccgata
    21001 tttcatcccg cttgtgttga acaggcgacg gattggtact tgactcgttt ctagccaagt
    21061 ctcaatcgtt gaggccttcg gtacttcttt gatcatatca acggtttgat aactaacgcc
    21121 atgctggtct aaccacgctt tggctttttt gcatgttgaa catttcgggt accagtaaaa
    21181 ggtgtacaat tttcttacct ccttgaagat cttgcaggtg cttcattttg gtatctcatc
    21241 gataatatca acccgatacc gatcatgttc cctaaaatcg aggatcctcc ctgactaatg
    21301 aaaggcaatg ggatccctgt taaaggaagt aatccgatat ttgctccaat attctcaaag
    21361 acatggaaca gcagcatcat gatcaaaccg cttgcgatat aggcgtaaaa ttcattattc
    21421 gtatcgaaac acacccgaat catacgatag attaaaataa aatacaagaa aatgacgaat
    21481 gcaccaccga taaagccgaa gttttcaccg ataacggtaa aaatcatatc ggactcacga
    21541 acaggcacat agatattgct tacgttgaat cctttaccaa acaagccacc ggtaccaatt
    21601 gccaaaagtc cttgggccgg ctgataactc attcctgacg tatcatggaa agggtccagc
    21661 catgaatcaa tacgtgcaaa ctgataagat ttgaatccaa ctttataaag caagtctctt
    21721 ccgccttcag tcgtaaccaa gaagatcgtt cctgctccaa cgactactgc caagattact
    21781 acaggtgcga tgatgcgcca agagattcca gacatcagga aaatcccgcc aaagatcgca
    21841 agaaagacaa gcatcgttcc aaagtcatct tgggccatga tcaatgctaa aaccggtatt
    21901 gtcacgatca agagcttccc aatcagccaa aagtccgatt tcatcgtccg ttcttttact
    21961 tgcgtattat gtttagttac gatcagtgcc atcatcaaga tgtacgcaat cttcatcaac
    22021 tctgctggct ggaaagtaaa cgcaccgaat cgaaaccagt ttttagaccc tgttgaagca
    22081 gctaaattct gatcatagaa tttgagcaag agtcccatta ccactaaccc caaaccataa
    22141 aggtaaggag tcagtttcca gatccatttg gaattcaaat gcatgatgac aacgattgct
    22201 gccgctccaa ctaaatacca gacaacttgc atccctacgc ctttcaagac tgctggattg
    22261 ttcggatcat ggctaagtgc tacatataat gagagcagac caatcaaaca taacagaaag
    22321 actggtaaaa tcacgccata atcaatacga ttatcgttcg aagcttttaa ttttttttcc
    22381 atgtttcatc ccttttctta aaaaccatac tgctgattat tatagcggtc cagttagata
    22441 atggaaagtc tttccataga catttgggaa aaattaactt tccgtttgtg cagttgcttg
    22501 agcaaatgca gtacaaaagc ctactgctta tctttagtgt atcatagaac tttttttgat
    22561 acatgcaatt atctaccagc tgagattctc ctcgctgtga gaagtttctg tgctttcatt
    22621 gtatcgaagg atctcttcgg caatctctag cagctgatca gaaaccagat tggttcgttg
    22681 gatgaaataa aaatatcgat ggaaattcaa cggagtcatt ggcacatttt ctggaacaga
    22741 gcgcttagac aaaatcgagc gaccgattcc ttccttcagc aacgcaacaa ttacatcgtt
    22801 acttttcacg atcatcttct tatcttggat attcatctct tcaaaataac gctgcgtgta
    22861 atgaaaaaca ccagagtttg cttctcttac aagccacaat ttgctttttg ggtccccagc
    22921 gatgactagc tggtcttcca tcaaagagta tctttgtacg ccggaagtct ctaatggctt
    22981 ttcgataaaa ccaaaatcgg attcatgttt cgctacttgt tctaatgatt catgagaatt
    23041 cgtgatttcg atctcaaaat gcactttagg aaaccttttg atcagatgtt tcatcaactg
    23101 aggcaaaaga tagacagcaa aggtgtggga agcaacgatc cgacatactt cttcttcatt
    23161 ttgttcattg agcaattttt gctgagtttc ttcccattct tctaacaaaa ccagtgtccg
    23221 ttcatataat aagtcggctt gcttagttgg aagcatctct tttcttccgt tacgttgaaa
    23281 aagatcgata ttcaattccc gctccaactg tttgatttga ttcgagactg ctggttgaga
    23341 taaaaacagt acttctgctg ctctggaaaa atttcttgtt tcatatactg ctcgaaaagt
    23401 cgttaataac ttaaacatgg caccacttcc ataacttttt atgataatta ctataaaaac
    23461 tatttgattt aaatatacaa aaaaccatct ataatagaaa tgtacaacaa aacagttgct
    23521 gatccaacta gtaacttaga tgaagacaat cagctgtctg cttgctcgta atcatacatt
    23581 cctattcatt ttacaatgta ttcgttcatc ttggtactaa aatctattaa ataaaaagga
    23641 gaatattata tatgaagaac tttttgaaaa ttattccgat cccgatctgc gggttgatcc
    23701 ttgggttagc ttcccttgga aatctgttta aagactatca ccatgttgct ttaggcaatc
    23761 taactggcgg tatctctatg attcttatga tattcatttt tgttaaattg tttatcttat
    23821 tcgaacacac gaaacaaacg ttgcatgatc caatcatcgc ctccgtttca ccaactttta
    23881 cgatgtcact catggttatt tgtacttatt ttgtttcata tgaaagtatt gcaccaattg
    23941 tcaaattcat ttggctgtgc gctgttatct tccaagtgat ccttgtcttt tatttcaatt
    24001 atcaccatgt tatcaaagct gatctttcaa ttgaagcaat ttatccaagc tggtttatca
    24061 tttacgttgg atttggggtc atcactgtaa ctgccgggaa cttcttccca cttattggaa
    24121 aaattttctt ttggatttca ttgatctgct atgcgttctt attgccgatc atcatcaaac
    24181 gtgtcttttt cgtaagaaat atggctcatc cgacacgtcc gctgattacg atcgttgcag
    24241 cacctggttc tctttgccta acaggttatc ttaaaaactt tgctgaacct aatttttatc
    24301 ttgtcgtagc attgttcgct ttatctcaag tattgtattt cgttgtgctg tctatgcttc
    24361 cacgtttgtt caagcttcgt ttttatccaa gctatgcagc ctttacattc ccgcttgtta
    24421 tctctgcaac agctatattt actactgttc attacttcaa cagtttaggt atctatagta
    24481 gcgtattgga tttcttaaaa gttgcggaat gtctgtttgc aagtgtagtt gtcttctacg
    24541 ttcttgctca ttacgtacgt ttcttaatta aagaacatag caaacatgga aaatttgttt
    24601 ctaaaaaaat cgcttaataa tcgattcaat aggttgtgaa aaagctgttc tgcatcaagt
    24661 aataagaacg gccaaacaaa aacagctgtt catgtttttg tactggttgg gcttattacc
    24721 gcagatgcaa gcttttgaac accgtttacc aagaggtcgt aa
//
LOCUS       DASJPC010000034        24259 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJPC010000034 DASJPC010000000
VERSION     DASJPC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.034"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..788)
                     /locus_tag="U4A13_001351"
     CDS             complement(54..788)
                     /locus_tag="U4A13_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN1779652.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="U4A13_001352"
     CDS             1115..1525
                     /locus_tag="U4A13_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN1779653.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="U4A13_001353"
     CDS             1518..2219
                     /locus_tag="U4A13_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN1779654.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="U4A13_001354"
     CDS             2219..3805
                     /locus_tag="U4A13_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779655.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="U4A13_001355"
     CDS             3824..4045
                     /locus_tag="U4A13_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN1779656.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="U4A13_001356"
     CDS             4042..4803
                     /locus_tag="U4A13_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN1779657.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="U4A13_001357"
     CDS             5183..5692
                     /locus_tag="U4A13_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN1779658.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="U4A13_001358"
     CDS             complement(5767..6306)
                     /locus_tag="U4A13_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN1779659.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="U4A13_001359"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="U4A13_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN1779660.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="U4A13_001360"
     CDS             7319..8170
                     /locus_tag="U4A13_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN1779661.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="U4A13_001361"
     CDS             8198..8833
                     /locus_tag="U4A13_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN1779662.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="U4A13_001362"
     CDS             8853..9626
                     /locus_tag="U4A13_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1779663.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="U4A13_001363"
     CDS             10034..10831
                     /locus_tag="U4A13_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN1779664.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="U4A13_001364"
     CDS             10809..11222
                     /locus_tag="U4A13_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1779665.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="U4A13_001365"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="U4A13_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN1779666.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="U4A13_001366"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="U4A13_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN1779667.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="U4A13_001367"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="U4A13_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN1779668.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="U4A13_001368"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="U4A13_001368"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN1779669.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="U4A13_001369"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="U4A13_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN1779670.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="U4A13_001370"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="U4A13_001370"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN1779671.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="U4A13_001371"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="U4A13_001371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN1779672.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="U4A13_001372"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="U4A13_001372"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN1779673.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="U4A13_001373"
     CDS             21906..22484
                     /locus_tag="U4A13_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1779674.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="U4A13_001374"
     CDS             22536..23018
                     /locus_tag="U4A13_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN1779675.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="U4A13_001375"
     CDS             23135..23509
                     /locus_tag="U4A13_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779676.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23939..>24259
                     /locus_tag="U4A13_001376"
     CDS             23939..>24259
                     /locus_tag="U4A13_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1779677.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         7951 a         4359 c         5182 g         6767 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
    23941 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
    24001 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
    24061 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
    24121 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
    24181 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
    24241 aaccactatt atccattta
//
LOCUS       DASJPC010000035        23208 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJPC010000035 DASJPC010000000
VERSION     DASJPC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.035"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..106
                     /locus_tag="U4A13_001377"
     CDS             <1..106
                     /locus_tag="U4A13_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1779678.1"
                     /translation="NTHIKTMKRVSYGFKSFENMRIRIFLINQLIKVR"
     gene            complement(300..755)
                     /locus_tag="U4A13_001378"
     CDS             complement(300..755)
                     /locus_tag="U4A13_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779679.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(909..2117)
                     /locus_tag="U4A13_001379"
     CDS             complement(909..2117)
                     /locus_tag="U4A13_001379"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN1779680.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2229..2561)
                     /locus_tag="U4A13_001380"
     CDS             complement(2229..2561)
                     /locus_tag="U4A13_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN1779681.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2709..3584
                     /locus_tag="U4A13_001381"
     CDS             2709..3584
                     /locus_tag="U4A13_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1779682.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3666..4247)
                     /locus_tag="U4A13_001382"
     CDS             complement(3666..4247)
                     /locus_tag="U4A13_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN1779683.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4253..5512)
                     /locus_tag="U4A13_001383"
     CDS             complement(4253..5512)
                     /locus_tag="U4A13_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN1779684.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5514..6560)
                     /locus_tag="U4A13_001384"
     CDS             complement(5514..6560)
                     /locus_tag="U4A13_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779685.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6795..7070)
                     /locus_tag="U4A13_001385"
     CDS             complement(6795..7070)
                     /locus_tag="U4A13_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN1779686.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7313..8623)
                     /gene="der"
                     /locus_tag="U4A13_001386"
     CDS             complement(7313..8623)
                     /gene="der"
                     /locus_tag="U4A13_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN1779687.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8805..10034)
                     /gene="rpsA"
                     /locus_tag="U4A13_001387"
     CDS             complement(8805..10034)
                     /gene="rpsA"
                     /locus_tag="U4A13_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN1779688.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(10169..10849)
                     /locus_tag="U4A13_001388"
     CDS             complement(10169..10849)
                     /locus_tag="U4A13_001388"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN1779689.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10898..11533)
                     /locus_tag="U4A13_001389"
     CDS             complement(10898..11533)
                     /locus_tag="U4A13_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1779690.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11599..13002)
                     /locus_tag="U4A13_001390"
     CDS             complement(11599..13002)
                     /locus_tag="U4A13_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN1779691.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12989..14020)
                     /locus_tag="U4A13_001391"
     CDS             complement(12989..14020)
                     /locus_tag="U4A13_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779692.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            14051..14272
                     /locus_tag="U4A13_001392"
     CDS             14051..14272
                     /locus_tag="U4A13_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN1779693.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14437..15174)
                     /locus_tag="U4A13_001393"
     CDS             complement(14437..15174)
                     /locus_tag="U4A13_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN1779694.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15327..16667)
                     /locus_tag="U4A13_001394"
     CDS             complement(15327..16667)
                     /locus_tag="U4A13_001394"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN1779695.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16904..18427)
                     /locus_tag="U4A13_001395"
     CDS             complement(16904..18427)
                     /locus_tag="U4A13_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN1779696.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18805..19416)
                     /locus_tag="U4A13_001396"
     CDS             complement(18805..19416)
                     /locus_tag="U4A13_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1779697.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19539..19664)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19746..20462)
                     /locus_tag="U4A13_001397"
     CDS             complement(19746..20462)
                     /locus_tag="U4A13_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1779698.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20468..21037)
                     /gene="scpB"
                     /locus_tag="U4A13_001398"
     CDS             complement(20468..21037)
                     /gene="scpB"
                     /locus_tag="U4A13_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN1779699.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(21021..21818)
                     /locus_tag="U4A13_001399"
     CDS             complement(21021..21818)
                     /locus_tag="U4A13_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN1779700.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21788..22159)
                     /locus_tag="U4A13_001400"
     CDS             complement(21788..22159)
                     /locus_tag="U4A13_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1779701.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(22178..23065)
                     /gene="xerD"
                     /locus_tag="U4A13_001401"
     CDS             complement(22178..23065)
                     /gene="xerD"
                     /locus_tag="U4A13_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN1779702.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6528 a         4952 c         3933 g         7795 t
ORIGIN      
        1 gaatacccat ataaagacaa tgaaacgagt atcctacgga tttaaatcat ttgagaacat
       61 gagaattaga atctttttga tcaatcaatt aatcaaagta agataacaaa aaatctgagc
      121 cagaatgagg ttcattctga cccagatttg attttgtaca acccatcagt ccttattgac
      181 aaagagccca aaaatcttgt cacagtccct tattctgaat aaacggtgtg gaagagacag
      241 cctatcagta gtaataagac acatctcttt cacagcctca tttttgcgta aaaatcgatt
      301 tattcgaaaa attccccgta tacatcttct acacgttgat ctggattgat cacgatatac
      361 aagttacctg ttttttcgct gatttttgtt gattgatatt gattgtctaa tccttctgtt
      421 cctttatcta gatatgggaa gtacacaaga tcaggtgtgc catttcggta aaggatttcc
      481 attcgctcga tatcttcgta tttcaatgcg cgattaaaca tgcccatttc cagtccgcct
      541 aggttgatat catgtgtcgc aactgcgtcc ccttcatgga agatttcgat tttaaatcca
      601 gcacacggat ggatttcaac aaattcactg ccattgattc gtccaaaact tgttgtgatt
      661 tgtttgatcc aaagatcgcc gatatgacgg cggttgatcg tccaggtttc tccatttcta
      721 aaataaaatt ttagcgctgt catttcttta ctcattaaaa agactccttt atgatgtaat
      781 ttattcttgt actacctcag tttagcacaa atttatgtaa gtggtcacat cttatgtttt
      841 ttcttctaat gacgtatctg cccactttaa gtagccaaca aagctgagca gatacagtct
      901 agtgtttttt aaatcaattt ttctttcgat aaaaaatcca aaacctgttt tttatcattt
      961 tttattcttt gttctacgac ggctttttca gctaattgca aagcttcact aacccactta
     1021 ccaggttttt ttccagtttc ttctagcaag attttaccat ctactgctaa ttcgcttaat
     1081 gacttgatag gtaaacgatc ataggattct cctacggcat ccatctgagg tgtttcgtca
     1141 aaatagatca tcaattcttc tacttgcagt gcatgttttt tgcctaagaa gtacaattcg
     1201 aatggttcca aagctccagt ttctcgttgt cgtagtcctt ggacaagtgc ttgaacgtct
     1261 ttaatcattt ggttagaaca cttccacgat ttcaaaaagg aacgaatctc attttctttt
     1321 agatccagtt ggctgatcaa taatgcccat gcttggctct cttctttcaa tgggacggat
     1381 ggtaattcag caaaacgtaa aagtgcttcg ccttcttctt tcaaaccggg acagcaaatg
     1441 tagcactctg tttcaataaa tgcatggatt cccgcggccc cttcatttcc caagagcatc
     1501 ttgatgaatt ccacatttat acgttctaca gatattttac ctaatagctc gtggttttct
     1561 ttgatggcgt agaaggtttc ttcctctaat ttgaacccta gttgactgac aaaacgcaac
     1621 ccgcgcatca ttcgtaaggc atcttcatgg aaccgctcat gaggattgcc tacagctcgc
     1681 aagatctgtt cttctagatc ttgcagacca tcgaacaaat cgatgatttc tccatcttct
     1741 ttcaaagcaa acgcattgat cgtaaaatct cttcttttca aatcttctgc caacgaacga
     1801 acgaattcga catgatctgg tctacggtag tcctgataag ttgattccgt ccgaaatgtg
     1861 gtgatctcat attgttcatc attttctaat accaacacag ttccatgttc gatcccaata
     1921 tctatcgttt ttggaaaaag ctgtttgatt tcttctggaa atgcgcttgt tgcgatatcc
     1981 acatcatgga ttggctggtt cagcaagata tcacgaacac ttccacctac aaaataagct
     2041 tcatatcctg cattctctaa ttttttcaga actggaattg ccttttggta ttccagaggt
     2101 aattgtttta atttcatttt tccaaccttt cctgcgtgtt tcttgtcctg agataaagac
     2161 tcagttgcat ggtgactgtt tgacaagtcc gtttcagagt cttcaaggcc aggaccactt
     2221 catcttgttt actcgtcctt acgctcaaag cgattatggt ctctttgatt gaatttttcc
     2281 atgttttttt caaaaacttc agttaagtcg atattcaatg aattagccat gatcatcgta
     2341 acgaataaaa catcaccaag ttcttctgct accgagttaa ctggttcaga agcctttttg
     2401 cttttttctc cataagtgtg gttgacttct cttgccaatt cacctacttc ttcagttaat
     2461 cgagccatct gagctaatgg agagaaatac cccaccttga attgctggat atattcatcc
     2521 acttcctgct gcatactgta taaagaacgc tttttatcca tctttttccc tccaaaacta
     2581 tcttaccaaa actttaacgc aaatcctaga taagagcaat tgagaaaatc gaatttccat
     2641 gctacattta ggaaggaatt tctaaatgga aattctgtga aaatacataa ttgaaagctg
     2701 gaattcatat ggtcaaacga aaaacagtaa aagaagttct ttttatcatt ataggaacaa
     2761 gtatttatgc atttggtctt gtttacttga acatcgctaa tcatttagca gaagggggag
     2821 tctcagggat caccttgatc ttaagagctt tatttggtat tgatccagct tatacgactt
     2881 tattaatcaa catcccattg attttactag gaggaaagat cctcggtaaa cgttccttgg
     2941 cctatactgt tctaggtaca ctttctttgt cattcttttt atggatctgg caaagggttc
     3001 cactagaaat caatttacag catgatctac tgatcgtttc tctacttgcg ggtttagttg
     3061 caggtgtagg aagcgggatc gtctaccgca tgggtggaac aacaggcggc agtgatatta
     3121 tcgctcggat tttagaacaa aattacggca tcactatggg gcgttcccta ttggcatttg
     3181 atatcatcgt cttacttgct tcattaacct atatcgactt gaaacgtatg atgtatacgt
     3241 tgattgtttc gtatgttttc agtcgtgtga ttgattcgat cttagatggc ggatattctg
     3301 caaaaggtct acttgtcgtc tccaacaaaa gcgaagagat tgcaccacta ctgatgaccg
     3361 gtctgcaaag aggtgtgact ttcctttctg gagaaggcgc attctctggt aattcaaaaa
     3421 aaatcatcta tatggtcgtt agctctagtg aattaactga agctaagagg atcgtccacg
     3481 agattgacga aaaagctttc ctttcaatca tcaatgtgca tgaagtggaa gggcaaggct
     3541 ttacttattt gaagccacag acaagattac tgaaaaaact ataagaaaaa agacaaagat
     3601 aggctatgag gaaagattcc acatccagct gacctttgtc ttttttagta ctatactatt
     3661 tttaactaat tgttatgttc acttacccgg ttacttttct tttgtgtctg caaaaggatc
     3721 tttcggttaa cttcatcaga taatttcaaa aaagcatccc tatctccatc ttctagtgct
     3781 tgatcgattt gagcataaag catttttagt tttgcctctt cttctttttc ttgaaaatat
     3841 cgttcaatta attcagtatc ttcttcactg actgaagaat cccaagaaga aaatggattg
     3901 tcttctaaga tagatagata ttctcttgtc tgccaagcat tttcgaatat acattccaca
     3961 tataaaggct ttttccaatt caaacggatt tcatgaaaaa tctggtctgt gtccgtaaat
     4021 tcttttccct gcaaaaacaa tttcatgggt tccccttcaa cagatagatc actgatctgc
     4081 agtcctcgat ccgcaaaatt cgccccttca acaaagtgga cattgcttaa aatcgcttca
     4141 tgattggcaa gataattcaa gatccaaaaa acttctcttc ggctaaaaga tacatgattg
     4201 accatccacg ttaaaaaatt cttcttttcc cgtacatcaa ttagcatttt tctcacctct
     4261 ctgttaggtc atctaataat gacagtgcct ccatatctcc tggttcaagt gttaaataat
     4321 aagtcaataa ttcttgcgct cttttcagct gcccttcttc tcttaagaaa aaggcatact
     4381 ctttcaagaa atctggttca tgtgatagtt catgataagc ctgttcatag tgtacagctg
     4441 caacggtaaa gtcttctaat tcattataag catgggctag gttccattct gcataaggat
     4501 tagcagtttc ttccatctga ttgatcactt gtatcacatc ttcatatctt tcatcatcca
     4561 agtaaagatt acttaaagta agtaatgttt catcttgttt gtctcctact tctaaagctt
     4621 tcagtaaaag ttcttctgct cgctgtctat catgcagacg gaaagcattt tccgaggcca
     4681 ggtgataaag ttcgacttgg taaggatttt ctttgattcc ttcttctaac actgtttgtg
     4741 cttcttcaat catttcttct tcttgaagag cttgtcctag atatagatat aacgattgat
     4801 aatgcggatt aacttctcga agttcttcca gatagttgat tgcctgctga ttttctttca
     4861 attgaagatt cgtaaaagct agctggaaca gtcgatcatc tgtcctttct tctttcagtg
     4921 cttcttctaa aggtacgata gctgcttcga attctccttg catacttaag gattgtccta
     4981 aacgttcctg catcgagatt cctgaaattt catttatccc gcttgccaac aatgtttcat
     5041 aaaccactgc tgcctctttg aaacgatcaa ctgaaaaata caattcagct agcgcaaact
     5101 ggatcaatgg ttcatcagga agcaacccag ctgcttcttt caattttgct tcactcactt
     5161 cagggattcc gataacttgg tacaaatcag ctatcgccaa taagctttgc ggatagtaat
     5221 cactttgctt atccactttt tccaggtatt caaaagctaa gtcgatctca ttatcttcga
     5281 ttgcaatttc tgctaacggg atgttcagtc cgtcattttc tgggtattgg ttgatcagtt
     5341 gttcaaaaat ctgttttgct tcgatcaaaa agccaatagc cagcaattct tcgcctagtt
     5401 cagctaatat atctgctggg tcttctttta atgctttttc taaaaagatt tgtgcttgtg
     5461 acaagtcttc atctgataaa gcttgcaaca ttttttcgct atttgtattc atttcaattc
     5521 cctcttatct cactgacgat ttgttgaaat accgtcattg ttctcaataa ttctttttcg
     5581 tttggatgtt ggtatccgtc tattatacct attttttcta aaacaagtat ttttttgctt
     5641 ttttctgcca aaaaaagaac tttttccaaa tactccatct ttgaaatctg ttccagccag
     5701 tggattggat agccaagcaa gtccagccac acaaaaaagt tttttagttg gaaatcaaaa
     5761 ggatatgctt ggagattcca cacgaaatga aacatcattc ctaaaaattt tttcatacta
     5821 gcagaaagta gatggccatt ttcggtcaga taaaaagctt gttcaaacag tttaccataa
     5881 tttgacaggt tttctgcctc taattgataa aaatagatca tttcttcgat catcccggca
     5941 aacggccgag tcgttaattg ctttttattt ggatagtttt tatacagatt ttgcaaaaaa
     6001 cgatgatcac aaatcattcc gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc
     6061 acctgccgct cgctgattgt ttgatcataa acgatgcgtt ctggcagatt gatggtttgc
     6121 agcactacac gatttggtag ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga
     6181 gaacggatcg atacagggat catccatagt gatgaatcaa gggttgtatg tttttgaaaa
     6241 aaacctgcca gatccatgat cccttcattt ccaaatccga tgataaacat ggattgattt
     6301 tcgggatagc gtttgacgaa atcaagcagg ttctttaatt ctgttaaatg attgcattga
     6361 gtgttggcac aaatatacca gtcattgttt gcttgatttg caaataacgg gttgatctta
     6421 tctgcaaaca aatcataata gcgctgattc gttaaaaata agattggctg atcaatggaa
     6481 agttctctta gctgactgac tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct
     6541 acttgataat caagttccat acgcatccct cctcttctta tttttatttt accattagta
     6601 agcaaattct agaaaagata agagcaaagc tcacctcata acttggaaat aaataaaaat
     6661 taaataaaat acatgtgttt tctatgtatg acatacggat tcggaatctc tgtctcttat
     6721 ttattccttt tctgctagga aataaaaaga aaagcacttg tagccctaga gccacaagcg
     6781 ctttttcaac tgtcttattt aacagcgtct tttaatgctt tacctggttt gaatgcaggt
     6841 actttgctag ctggaatttc gatttcttca ccagtttgtg ggttacgacc tttgcgagct
     6901 gcgcgttcgc gaacttcaaa gttaccaaaa ccgattaatt gaactttttc gccgttagct
     6961 aaagcatctt gaatagttga aaatacagca tctactgctg cagttgcatc ttttttagtt
     7021 aaatcagtag ctgaagcaac tttttcgatt aattctgctt tgtttgccat ggatagattc
     7081 acctcctcca caagagatat gatagaaata tcatttagtt aaaactttct gagtggtcca
     7141 gaagtttaaa aagaaatatt gattaacttc aatattctgt tatcacgata tcatatgaac
     7201 cgcttaatag caaggttttt accaattttc acatgctttt tttagtcata gaaacgtaac
     7261 aaacgaaact gtctttttct tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc
     7321 tcctacgagg gataattcgg atggacgtcc cttcaaaagt aaatgctttt ctgatttgat
     7381 tttctaagaa acgttcataa gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa
     7441 atgtaggtgg tttgacagcg acttgtgtag cataaaaaat cttcaggcgt tttcctttat
     7501 ctgttggtgt tggattgatt gctacagcat ccataatgat atcattcaac acagccgaag
     7561 agatacgaag attctggttc atacttactt gttcgatcat ttcaggcaga cgttccaacc
     7621 gttgtttagt cactgcagaa acaaaaacga ttggagcgta atcaagatac cggaattcat
     7681 cacgaatctc ttgttcgaaa tcacgcattg tattggtttc tttctttaca agatcccatt
     7741 tattcacgac gataataatt ccacgaccag cttcatgggc gtaacccgct actcgcttgt
     7801 cttgttcccg aatcccttct tcagcattca gtaccatcaa aacgatatca gaacggtcaa
     7861 tggcacgcat tgcacgcatc acgctgtatt tttcagtatt ttcgtatact tttccgcgtt
     7921 tacgcatacc agctgtatct atcattaaga atttctgacc actttcagaa acgaagtggg
     7981 tgtcgattgc gtctcgggtc gttccttcaa tatccgaaac aattacacgt tcttctccta
     8041 aaatcgcatt gatcaaagag gatttaccaa catttgggcg accaatcaaa ctaaacttaa
     8101 tcgtgtcgtc gtcctcttct tcagtctctt cagagaaata cttcactgct tcatctaaaa
     8161 tatcaccaat ccctagtcca tggcttcctg aaacaggata aggatcgcct agccctaagg
     8221 aataaaattc atagatatcg tttctcattt ctggattatc tactttattg actgctaaaa
     8281 tcactggttt attacttctg tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc
     8341 cttcgcgtcc gctgactaca caaatgatta catctgcttc ttcaatcgcg atttctgctt
     8401 gatgcttgat ctgatccata aaaggttcat cgccaagatc aattccgcct gtgtcgatca
     8461 cgctgaattc tcgacctaac cattcacctt ttgcataaat acgatcacga gttactccag
     8521 gagtatcttc gacaatcgag atacgctcac cagcgatgcg gttaaaaatc gtagacttac
     8581 cgacgttcgg gcggccaacg attgcaattg ttggatttgc catattattt cctcctcaca
     8641 agtttccaat ctcacttagt ttaacgaatg cattgccaac tttcaagcat agattgtcaa
     8701 ttaaacgaag tatttttatt ccttattgaa atactaatcg cttttcaaaa gaaaaactga
     8761 gacaaaagca gctgctttca tctcagtcac agatacaaaa tcaattattt ttcagaatca
     8821 tcggttgctt tttcttctac ttcttctttc aacgcatcac ctaaaatgtc tcccatagta
     8881 aatccagtat tttcttctgg taattcataa gactcaacat tttttggttc ttcttctgaa
     8941 gctggttttt cttctaaagc tttgatactc aatgcaatac gatgttcttc tggatggatc
     9001 tctaatactt tcactttcac ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata
     9061 tgtttatgtg aaatttgaga gatatggact agcccttcta ctccagggaa cacttcaaca
     9121 aaggcaccga agcttgtcaa acgtttaact gttccgtcta atactgtgcc aactggagct
     9181 tttgtatcga tttctgacca aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt
     9241 tcttgttcag gattgataga caacactttg acatttacat catcattgac agccaataca
     9301 tcacttggtt ttgctacatg actatgtgaa atctctgaga catggactag accgtcaatt
     9361 ccgcctaggt cgacaaatgc gccaaaatca gtaagacgtg caacacgtcc actgagaaca
     9421 tctccctcat ggatagttga aagaatttct tttttctttt cttctctttc gttttccact
     9481 accgctttat gcgataagat caaacgattt tctgatggtt cgatttcgat gattttaaag
     9541 ctcaatgttt tgcctttata gtctgaaaag tctgcaacaa aatgatcttc caccattgat
     9601 gcagggacaa aaccacgtac accgacgtca acgactaaac cgcctttgac aacatttgta
     9661 acaggtgctt cgatgacctt gccctcttgg aaatctcttt cgatatcttc ccaaactttt
     9721 ttagcatcta aacggcgttt tgaaagtagg tagctgccgt tttctttatc tttgccgatc
     9781 gtactgataa cgactaaatc taatacatca ccgactttta ctacttcgct gatatcttct
     9841 accggcaaag ttgataattc ttttgccgga acgactcctt caactccagc cccttcgatt
     9901 ccgacaatca cttgtttgtc ttcaatgact aaaacttcgc ccttaacaac atcgccaact
     9961 tttacttctt gtacgctttt cattgcgtct tccattgact ggttgttctc ttgattttga
    10021 tcaaattctg tcataacact tgtcctccta accaacattc tgccgtaagt acggtactgt
    10081 ttttagttta attgattatt caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa
    10141 acaattgttt ttcatttttt ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa
    10201 cagcttttat tgctgcaacg acttctggaa tcgatttgcc agtcgtatcg atacgaacag
    10261 catcctctgc ttgcttcagc ggagagacgt cccttgttga atcataatgg tctctttcct
    10321 gtatctcttt cgtcagtgtt tcaagatcag tcggaattcc tttttcttgg ttctctttga
    10381 aacgtctttc tgcacgctca cttacgcttg ctactaaaaa gattttcact tctgcattcg
    10441 gtaatacagc tgtcccgatg tctcttccgt ccatgacaac gccaccgcgt tcccctattt
    10501 ttcgttggac agcaactaat tcttctctga cttttccatg tgcagagact tcagaaacag
    10561 cttttgtgac atctggttga cggatttcta aagtcacatc ttttgatgct atgaatacat
    10621 gctgtccatc ttcttgttgc gaaaaagaga taggatactt tttgatcagc gaaactagcc
    10681 cttcttcatc tgaaaaagat acattgttcg taatagctaa ataagttact gcgcgataca
    10741 tggcaccggt atctgtataa atataattca attcttttgc taatatcttg gcaacggtac
    10801 ttttcccaga tgaagcaggc ccgtcgattg caatactgat ttgtgtcatg atataactcc
    10861 tttttctcag aaaaaaagcc gcaaagcgcg gctattatta tttaattctt aattcttgac
    10921 cagggtaaag cataaagttg tttggatcta agccattcaa ttcaaacaat tgatcagtgg
    10981 tgatccctgc acgctcagcc acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg
    11041 aaccggctgc agctgatgaa gaaggtgttg tttctgctgc attgctgctt gaatcccctg
    11101 cttgataagt tgcctcactt gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg
    11161 acgttacacc tgtatcttga gacacagtag attcttccgc gattgtacta gattctactg
    11221 ttgaagtctc agttgactcc tcttttgttg tacttgtaga actttctact actgatgtcg
    11281 aagtttcaga agaagttgct gctgttgcat tgttgcttga tccatttctg atccagaaat
    11341 aagcacctgc tggaatagcg atacataaga ccagtaaaat caaaaatagc gaaagaaata
    11401 atgtatttcc tttcttttgt tgtctctgag aacttcttga tgaaacttca tcatctgtat
    11461 cgtagattgg ttgttcccat ggttcttgtg tttcgttatt ttctgtagat tgatgtctat
    11521 cttttctgct cactgtttta cctcctaaac tctatcgtgt gtattatacc ataggaaaaa
    11581 gtgtcttgtc ctaaaaaatc aatgttccat gtttttttta aataaattta gtaaaatcgt
    11641 ttcccaatga agttgttcag tcttagaaaa aagcttcctc tttggcagaa gttctgcttt
    11701 tgctccatca atatcacaac aattctgcgg cttttcggag agtttttcgc caaaataagc
    11761 taagatgaat tttctgcggc agttttcttc attaatatag cgaagcatct gctgcaggcg
    11821 gaattctttt tctttttcct gacgtttgag ccgttccacc caattttctg gtttttcaga
    11881 ctgctggatc agctccatcc attttttttg cagctcatca aagggagctt gctttgtggc
    11941 atattccaaa taaagctcaa aactttgtcg ctgttctcta gaaagttgat taaagaaata
    12001 atggatacgt tcatccccac tttgataaaa aagaattgct gcactttctt cctgatctct
    12061 gcctgctcga ccgatttctt gtacatagtt ttctagactg tctggcaaat cataatggac
    12121 cacataacgg atatcagatt tatcgatccc cataccaaat gcattcgtag caatgagaaa
    12181 ctgcagctga ttttttacaa actgctgttg aagttgtctt ctttgagctg catcaagacc
    12241 accgtgatag tatccaactg taaaacgttc cctgaataaa tgatacaatc gttcgacttc
    12301 tttttttgtt gcacaataaa tgattgccgc tccttgtgct ctttccatga agtgttcaag
    12361 ttcttgttct ttttgctgcg ttttccgaac aaaaagcgag atattttgcc gattgactga
    12421 ttgtctgatc tcttttggtg gtcgatcaaa tagtacatga cgtatatctc ttgctactaa
    12481 atctgttgct gtcgccgtta gtgccagtgt gaccgggttt cctaaaagct ttcggatttt
    12541 tcctaactgt tgatattctg gacggaaatc aactccccat tgagacacgc aatgagcctc
    12601 atctactaca tataacgcaa tattttgttt tttcagatgc tctaaaagag aaggattagt
    12661 caacatttcg ggactcaaaa acaaaaattt atattgcgat agatgacgga ggacgtatct
    12721 tctttcagat tgcgaaagta aactattgaa agcagctact cgtttctccc ctcgtttttg
    12781 taattgcgtc acttgatctt ccattagaga aagcagcggt gacacgatga tgaccagacc
    12841 ttccattaaa taccccgtca attgatagca aaggcttttt cccgttcctg taggcaaaat
    12901 agcgagagta tcttctccat caagcaacgc ctgaataatt tcttcttggc cgggacgaaa
    12961 tgaatcaaat ccaaaatatt tttttaattc atttattaac attctgtatc gcttcctttc
    13021 tcaatagata gatttgatac aaacgaaatt ctccataatc taattgtcca tctacattcc
    13081 attcgctaaa gcgccactct cgcacggaat ccttatttgg ttccaaacgt tcgattgttt
    13141 ctgcagagag aatccactcg tatggaaaat catcaaaaaa caaggcccac tcaataagat
    13201 gatctgttac tgttcctctt tttaaatgac ggattgccat gatctggtca attgtctgac
    13261 cattcataaa catctgcttg gtgatcatca tgctttgatt gaaattttgc tgtaataatg
    13321 aatcgatcaa agcgtgccaa agcgaatctg gccgtttttc gatttgtgct aacaaaaggt
    13381 ggatacatct gctttgttgc agataaacct ctgactcatc attgtttttt gccagctgat
    13441 aaggaagcag ccctgttcgc tcatttccag ataattggtt agctaaaaaa tctgctgctt
    13501 cttctggtat ctccgaaaac agctgcacca aactctcata cgcagtatca atcaaaattt
    13561 cacgtggcaa ttggctttca ctaagccatt tttttagttg gaaagtataa aaaggacttg
    13621 tctctgctgg aagatagtct tgaatacccg ttgccagatt agaaattact tgaacagcaa
    13681 atttaatgag ccgccaacta gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag
    13741 tatgttctat ttgatgttcg gacaacatat cagcccctgc aggcgttagc tttgcttcat
    13801 tttcatttat ttcgatccaa ccttgctgcg caattttttg caaaatttga taaaacttat
    13861 cctgcttcaa ttctggaaaa ctgttatgga taaacaaaag ttcgtgggta aatccataaa
    13921 taagtactga agtcgttcgt tttcccacaa ggagttggta aagagaacta atccgcagct
    13981 tgtgtcctga tgaaaatagc gataaaataa aaacaatcat attattctcc ttaagttagg
    14041 atggttagaa atgtcactac tatgtaaact tgtacctgaa cgctgtattg catgcggtct
    14101 ttgtcaaatc gaagcgccag atattttcga ttatgatgaa gaaggaatcg ttttatttgc
    14161 ccaagaacca gaggctcatc aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa
    14221 agcgtatcaa aaatgtccag ttcgagcgat tttattagaa aataaaatat aaatataaac
    14281 gaaactttaa aaacgtacac taagttctct aaacttgaaa ataagccggg atctgtttct
    14341 cttgttccgt gaaatcccga cttattttga aagattcatt tgctatctat agttaattga
    14401 ctagtttttt cacggaccct ataggaccca tttatattat ttcaactttt tcagccagtt
    14461 ttccaatgta ttgacgatca cagtttgctg gtctgcgtta gaaatttctg cctctccatc
    14521 tcctttttgc tcaccatagc tgccaaaacc tccatgattt ccgccattga ttgatacata
    14581 ctctgttctt tccggtaaat actgcttagc ttcttgataa gcttcctgat tcaatacttt
    14641 atcttttgtc gccgtgatcg ataaaacagg aacagctgat tctgtcaatg ttcctttttt
    14701 gtcgggataa cttgccatga agaatacccc ttttaaatgt ttgtctgaat gattcttatt
    14761 gacataacga cttgccatta cgccacctaa tgaatggcca ccgatcacat aatcggaatt
    14821 tggaaaatct tctttgacct tatccgcttt gtttccgccc atcactgcta gatcaagtgg
    14881 cattttgaca atagcgactg aaaagccgtt ttttgctaaa tcttgcgccc aaatactata
    14941 actttctggc tctacgagag caccaggata aaaaataagt aatggagact ttccatgatt
    15001 ctttttctct ccttcaaaaa ataagtaatc ttctttgttt tttccttctt ttaccaacac
    15061 ttgcgcttct gacgatggtt cgtacgtatt ttgctgtata tagtaataac cggctccacc
    15121 gagtaatcct aaaatgatga ctaagccaat tagtattcgt ctaaatattt tcactttctc
    15181 ccaccttttc tatctgagtg accccttttt cttaatcaag tatgattata ccttaaaaaa
    15241 ataagagaga aatgcaaact ttccagcatc aaataaaaaa agacggaata tccatttgaa
    15301 tatccgccct ttttgtatgt ttatttttat ttagcacata aacgatacac agcttctgca
    15361 taaatttcca ttgctttata catactactt aattcccatc gttcattcgc ctggtgcata
    15421 aagtcaggag tatcagggaa catcgcaccg aatgccacac actggttcat cgtacgagca
    15481 aaagtagcgc cgcctgatac aaacggttca gtcatgtctc cagtaatatc gcgatatgtc
    15541 cccaatagat ttttcactaa ctcactgtct agtggcacgt ataatgaatc aaggaagtca
    15601 aattcttcat aagtcaaccc atatttttct gctgttttag ttaatttagc tactaaatca
    15661 tctttcttga atgtaacagg gattctcata tctacaccga tttttgtttg ttctggtgtg
    15721 atttccaaac ttgcaaagtt catcgttagc tcgccggatt gttcatcttc tgttttgccc
    15781 acgacagctt caccagttgc attttcttga actaattttc ctaagaaatt gattgggcca
    15841 aaatcaaata cttctgaaag agcaattgcc agacgagaaa tagcattcac tccttgtgct
    15901 gcatctttcg cgtggatact tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa
    15961 tcgaattcgt gttttttcaa tgcttctttt acttcagaaa gcttttctcc agagtaagga
    16021 gctgcatcag gaacaacatt caacgctcct ccagctttta cagaaaactc gttcgttcct
    16081 ggtcctgtca agtaagcttg caataagcct ttttctgcat aaattaaagg aaattcagca
    16141 tctggcgcaa atccttgcgt aattccttct tctttttcgt tatatttttc taaacaacgc
    16201 cataaatttt cttcatctgt accaaagata aaacgaatac gtgtcttgaa ttccacgcca
    16261 gcatcaatca aagctttgac ggcatacatt gccgcaatag aaggaccttt gtcatcttgg
    16321 gatccgcgac caactaacca gccgtctttt atagttggat cgaatggttt tgtttcccaa
    16381 ttattttcgt cccctgctgg aacaacgtcc atatgacaca agataccgaa taattcatct
    16441 ccagagccaa tctctgaata accgtaatag ccttctggat ctttaaatgt tctaaatcct
    16501 agcttttcac tgatttccaa tactttatcc aaagcttcaa tcactttttt accgaaggga
    16561 tgtccttgtc ctgaatctgc ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat
    16621 gattctaatg cttgttcatg atgttgttct gtcacaaatt ttttcatgat attatcctcc
    16681 taatatgtta aaaagggtca tgtcaaattc ctccgacaca acctcttctt ctcaaataat
    16741 gacgttttaa gcctgctcca tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa
    16801 acactcactg aatggagcta ttgacttgtc cacgtgctta tcgatagccg tttatcggtt
    16861 tcttcattct tatgatagac cttgtacctg tatttgtcta ggtttagaac attgctgcta
    16921 cacctaggat cacacagatg gctacaaata atatcgcaat taatttaccg gtaaatttcc
    16981 accacactga aacatcgaaa cgagcaattg ctacagcacc cattacgata gcagatgttg
    17041 gtgtaattaa gtttactaaa ccagatgcag attgataagc agtgattact aaatccttac
    17101 cgacgccggc aaattcaccc ataggtccca tgattcccat tgttgcagca gctaagccgg
    17161 aagttgaagg aatcaagaat gacataggga tatagaaaat aaatgtaata atgatgaata
    17221 cacttgaaga taatgaactt aatccttgtt cgccccaatg taagatggtg tctgtgatta
    17281 atccattgtt cataaccact tggataccac gagcaacagc tactacgatt gctacaccga
    17341 tcaaatcaga agcaccagct aaaaattcag agacaaattc agactctttc atgccataag
    17401 tgattgctac tacgatggac attactaaga ataacatagt aatttctggg aagtaccagt
    17461 cacctaatgg aagcatgctt ttacctagaa ccgtacctag taccggaagg tttgttaacc
    17521 agtttgtcaa tgattcaaaa atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac
    17581 tgatgatcat gataccaaaa gtaataaaga acaatacatt aacatgtttt tgttttttcg
    17641 tgattacttc ttgacgaccg attgcttcaa tatcgaaatg cttcatatct tctttacgtt
    17701 ggttgtatac taatgatttt gttggatctt tttcgatctt tgatgcataa cgatacacat
    17761 atacaataga aatagctaac aaaataacga acatcaagaa tcgaagacca ataccttcac
    17821 ccatcgaaat cccaacagct tgtgaagcta cgcctgttgc aaaaggattt accgttgaag
    17881 ctagacagcc tacttgagac cctactaata cgattgctac agctactact gtatcaaagc
    17941 ctacagccat catgactggg atcagtaaag catagaatgg aatcgtttct tctgccattc
    18001 cgtaagtaga gccgcctaat gcaaataaca gcatcaatac tggaataagc attttttctt
    18061 ttcctttgaa tttcttcaca actgaagcaa tcccggcatc tagtgcccct gttttgttga
    18121 taatacctaa aaatccacca acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg
    18181 tttctttagt tagctccccc gctggtacac ctagcatccc attgattggc gccatgaaaa
    18241 tatcccacag cccttgagga ttttgttgta ctcgttcata tgatccagcg acgatgttgc
    18301 ctgcatcgtc tacactatag tgcccagctg gtataaacca agtaagtaca gcgattactg
    18361 caataataat aaataagacc gtatacgctg aaggcatttg aaattttttc ttttttgttt
    18421 gttccatctt cttcttcctt tcttttccgt tagagacaac aattttggct ctaatgttat
    18481 tttatcaaaa agagtatcag aacaaaaata ataacacgca ttttgtaaaa cttttgttca
    18541 aaaagttcgt ttttccctat tccctcctct tttttcagtt ttttaaagct ttcttctttt
    18601 tcattgtagc gtttcctttt ttttataaac aaacattttc gtcttttaaa aggagaagaa
    18661 tttcacattt gcaaagaatt gattgatttt tatgcataaa tcaaaaaaat actcaaaaca
    18721 aacaaataaa acgtttacat tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa
    18781 gaaatttctt gatctttttg atatttattt gactgctgta tgacgttgtt cgaactgatg
    18841 ttgttttcga cttaaccatg gaagaagttg cgcatgcagc actaaaaaga cagcactcac
    18901 gatgaatcct ttgatcaagt tgaaaggaac gatcccaatc agtacatagt ttgctaaaga
    18961 catgcccagc atctgtgttg cgttcagccc taaaaacatt aagtaaagag gcgtaataac
    19021 gaaataattt gcgatactca taaaaatagt tagagcgatg gttcctgtaa tgacacctaa
    19081 aaatttgttt cttcctttat gctgtttctt tacactaaag aaataataaa tcgggaatgt
    19141 aaagatcgtt gttgataaga aactagcagt gtctcctacc aaattatccg gagaaaaacc
    19201 tgtagtcagc agatgtaata ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc
    19261 gaataagaac atgctgatca ataccgagat atcactaaaa tcgatcttca aaaaactaaa
    19321 agccggcatg atcggaaaag caaaatactg taaaactaat gcgatcgcag caaacatcgc
    19381 tactgcaacc attttctgta cacgggtgtt cttcatttga aatcctcctg ttaggactcc
    19441 tcttcagcga actttttgac gtttctctcc aaatgaaaaa actctatttt ccagggaaaa
    19501 tagagttaga gttatttgat caagcgtcgt caaataagct ctatcttctt tatccagact
    19561 atactgtcgg tatcggaatt tcaccgattc agctattttt ttgcaaaata ggtcgtggac
    19621 tataaccacc ggtcgggaat ttcaccctgc ccctgaagac gaaccttata taatttttgt
    19681 ttacatattc attatacata tctattcaaa aaacgcaaca tctaacaatt ataaaaagta
    19741 agtgttcatt ttgagtggtt cagcaaactg ctgacttctt ttttgttcag ttcacggtaa
    19801 tccccaggac gtaggccttg taaagtcaga tctccgtatt tttcacgttt tagtttttgc
    19861 acaggaagac caactgcttg aagcatattt ttcacttgat gattacgtcc ttcgtggatc
    19921 gtcaattcca cgatactgtg gtttgtctta ggatccactg ataaaatttg gaatttagct
    19981 ggagatgttt ttcgtccttc gatctttatt ccttttgtca aaggtgcaag catcgatttt
    20041 gtagcgatcc cttttacctt tgcaacgtaa actttatcca cttcatgttt tggatgtgtc
    20101 aggcgctgag caaaatctcc atcatttgtc agtagcaaga tccccgaagt atcgtaatcc
    20161 agtcgaccta caggatagat acgttctttt accatcggca aaagatccgt aacaactttt
    20221 cttcctttgt catctgcaac agcagaaata actcctttag gtttgtacaa taaatagtaa
    20281 ccgtattctt cttgataaat tgggacttca tccacttcca cagtgtcatg tttcccaact
    20341 tttatcccta attcccttac aacttgtccg tttactttta cacgtcctgc aaggatgaat
    20401 tcttctgctt ttcgacgaga agtgatgcca gcgtgggcaa ttactttctg caatctctcc
    20461 attacaatta ttctccttta ttcattccat caaagaatag atctggtgac acgtcttctt
    20521 caagtgaaat ttccatttct tgtgtatcag gcagttcttc taatgatttc aaaccaaaat
    20581 aatctaagaa gtagccagtt gttccatata atatcggccg acctggtcct tctactctac
    20641 ctttttcttc aatcagttta tgggcagcta gacgctgtac cgctcctgaa acctgaactc
    20701 ctcgaatctg ctcgatttca gctcttgtga ttggctgttt ataagcgatg attgctaagg
    20761 tttcgacggc ggcttgagat aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg
    20821 gagccagctc ttttttagtc gttaatacat aatggctttc cgtttcaaaa atatgcaaag
    20881 ctgagtgcga gttttgagtg taagattctt ttaattcttt cactaaactt gtgacttctg
    20941 cttcgctgag atttaacagt ccgcttaatt cttcattact gatcccttcc tcgccaacaa
    21001 taaaaagaag ggcttcaagt tcacttaatt tcgtcatgtt cggtatctcc tactgctgga
    21061 taaagcataa ttgattcata gttttctttt tgttgcgcaa tgatctgccc ttttttcatc
    21121 aattctagca aagccataaa agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa
    21181 gaatcgaaag tacagccatg cgcatttctt tttatacgct cttcgataaa taccattcgt
    21241 tcttcgatcg ttgtgtcatc gggcgtgatc gttttttcca ctttttgaga aaatttctta
    21301 cgttctaaga ttttctgcat ggcacggatc aaatcgattt ttttgacttg tcctcgcttc
    21361 aacggctttt cagcatccgc taacccatcg atgttcatag gttcttttgt gtaataaaga
    21421 cttcgttcct ctgctttttc tgtcagtctt tcagctgcat atttatattt acggtattct
    21481 aaaagctgag tgaccaattc ttctcgagga tcttcttccc ataattcttc atcgtctgtg
    21541 ccgaattctt ggacaggaag caatgtcttg cttttgattg ccatcaatgt agcagccatc
    21601 actaagtatt ctcctgcaac agctagctcg agtgttttca ttgtatggat ataattcata
    21661 tattgctccg tgatcgtagc gatcggtata tcatatatat cgatttccat ttgctgaatc
    21721 agatgaagca gcagatcgag aggaccctcg aatacatcta atttaatatt gatctccgtc
    21781 aactcattca ctctttcttc tttttctttg ctagccattt tccaatgtaa ggctctgtga
    21841 tttcagttgc acggatggag acatccgggt acttttcctg taattcatct aacatttgaa
    21901 agcccatatt ctcaccgcga taagacggat tcaaagaaat gtcatggatg atcagctgat
    21961 cgttcgttgc ccagtaaata ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa
    22021 agaaaaaaag ttcataatcg tcttcagatt cgtacgtatc aatctccttc aacaaagaag
    22081 ttttacctgt cagtttctta tggaaactca atagtcccat agcaatttta cggttttctt
    22141 tccggtaatg tgttaacatc ttctcacctt cctatgacta agcccttgga aaatgctgct
    22201 tataaacatc cgtcattctt ttcttcgtga tatgtgtata aatctgagtc gtagagatat
    22261 ccgcgtgacc taacaattcc tgtactgtcc gcaaatcagc tccattttct agcagatgtg
    22321 tggcaaagct atgccgcagc gtatgtggtg ttacttcttt gtaaatccca gcctctcgta
    22381 ctaactgctt cagatttttc cagattcctt gccgagaaag acctttccca tgattattga
    22441 caaatacatg catttcagat gcgtccttta caagtaatgg acgtgcttcg tctaagtagc
    22501 gttccagcca ttggatcgca taatcaccta aaggaatgat ccgctcttta tctccttttc
    22561 caactgtctg taccagtcca agagaaagat gaagatctcc caatttcaat ccaatcaatt
    22621 cactgacgcg catcccagta gcatacatga cttccaagat tgcacgatct cttatcccta
    22681 acgtttttgt cgtatcaggt gcttcaatca gcttttctac ttctgtcaga gtcaatgtac
    22741 ttggtaattt ctgtgctttc ttcggcgtat cgatatgttg catcggatca tgatcagtga
    22801 tacgctcttg ccgtaaaaac tgatggaatc ttctgagact tgagatcatc cgagtaacag
    22861 ttgctgtcgc ttttttttct tcatgcaact tctccagaaa acggatgact aaaaagcgat
    22921 cgacatcctg ccatgaagca attttctgct cctgtagaaa atgcaagtac tgttccaaat
    22981 cacgttcata gctttgtcgt gtattggcgg acaatccccg ttcaatattc aagtagtgga
    23041 gataatcgtt gatttgctct ttcataataa tccccctcat gcctctctat catatcagat
    23101 accgcttcat tctgatagtc tttttccata atctgagcaa agaaaaagcc gtctagacac
    23161 tcagacggac tggtttcttt tttcggctct ataatttatg ggactgat
//
LOCUS       DASJPC010000036        22533 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJPC010000036 DASJPC010000000
VERSION     DASJPC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.036"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            90..491
                     /locus_tag="U4A13_001402"
     CDS             90..491
                     /locus_tag="U4A13_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1779703.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            485..730
                     /locus_tag="U4A13_001403"
     CDS             485..730
                     /locus_tag="U4A13_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779704.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(1054..1275)
                     /locus_tag="U4A13_001404"
     CDS             complement(1054..1275)
                     /locus_tag="U4A13_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779705.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(1437..1509)
                     /locus_tag="U4A13_001405"
     tRNA            complement(1437..1509)
                     /locus_tag="U4A13_001405"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Lys,seq:ctt)
     gene            1585..1815
                     /locus_tag="U4A13_001406"
     CDS             1585..1815
                     /locus_tag="U4A13_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779706.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            1859..3028
                     /locus_tag="U4A13_001407"
     CDS             1859..3028
                     /locus_tag="U4A13_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1779707.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            3602..4171
                     /locus_tag="U4A13_001408"
     CDS             3602..4171
                     /locus_tag="U4A13_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN1779708.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            4158..5018
                     /locus_tag="U4A13_001409"
     CDS             4158..5018
                     /locus_tag="U4A13_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1779709.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            5208..5912
                     /locus_tag="U4A13_001410"
     CDS             5208..5912
                     /locus_tag="U4A13_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1779710.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            5917..7752
                     /gene="walK"
                     /locus_tag="U4A13_001411"
     CDS             5917..7752
                     /gene="walK"
                     /locus_tag="U4A13_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN1779711.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            7749..9065
                     /locus_tag="U4A13_001412"
     CDS             7749..9065
                     /locus_tag="U4A13_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779712.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            9066..9935
                     /locus_tag="U4A13_001413"
     CDS             9066..9935
                     /locus_tag="U4A13_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN1779713.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            9990..10799
                     /locus_tag="U4A13_001414"
     CDS             9990..10799
                     /locus_tag="U4A13_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1779714.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(10902..11531)
                     /locus_tag="U4A13_001415"
     CDS             complement(10902..11531)
                     /locus_tag="U4A13_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN1779715.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            11701..12993
                     /locus_tag="U4A13_001416"
     CDS             11701..12993
                     /locus_tag="U4A13_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1779716.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(13069..13731)
                     /locus_tag="U4A13_001417"
     CDS             complement(13069..13731)
                     /locus_tag="U4A13_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1779717.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            13956..14240
                     /gene="groES"
                     /locus_tag="U4A13_001418"
     CDS             13956..14240
                     /gene="groES"
                     /locus_tag="U4A13_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN1779718.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            14291..15916
                     /gene="groL"
                     /locus_tag="U4A13_001419"
     CDS             14291..15916
                     /gene="groL"
                     /locus_tag="U4A13_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN1779719.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(16005..17153)
                     /locus_tag="U4A13_001420"
     CDS             complement(16005..17153)
                     /locus_tag="U4A13_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1779720.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(17255..17839)
                     /locus_tag="U4A13_001421"
     CDS             complement(17255..17839)
                     /locus_tag="U4A13_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN1779721.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(17966..18397)
                     /locus_tag="U4A13_001422"
     CDS             complement(17966..18397)
                     /locus_tag="U4A13_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1779722.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(18415..18735)
                     /locus_tag="U4A13_001423"
     CDS             complement(18415..18735)
                     /locus_tag="U4A13_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779723.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            19035..19175
                     /locus_tag="U4A13_001424"
     CDS             19035..19175
                     /locus_tag="U4A13_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779724.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            19473..19730
                     /locus_tag="U4A13_001425"
     CDS             19473..19730
                     /locus_tag="U4A13_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEN1779725.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(19701..20009)
                     /locus_tag="U4A13_001426"
     CDS             complement(19701..20009)
                     /locus_tag="U4A13_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779726.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            20087..20833
                     /locus_tag="U4A13_001427"
     CDS             20087..20833
                     /locus_tag="U4A13_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN1779727.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            20848..21051
                     /locus_tag="U4A13_001428"
     CDS             20848..21051
                     /locus_tag="U4A13_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779728.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            21156..21455
                     /locus_tag="U4A13_001429"
     CDS             21156..21455
                     /locus_tag="U4A13_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779729.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            21455..21637
                     /locus_tag="U4A13_001430"
     CDS             21455..21637
                     /locus_tag="U4A13_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779730.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(21643..22119)
                     /locus_tag="U4A13_001431"
     CDS             complement(21643..22119)
                     /locus_tag="U4A13_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779731.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            22172..>22533
                     /locus_tag="U4A13_001432"
     CDS             22172..>22533
                     /locus_tag="U4A13_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEN1779732.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIE"
BASE COUNT         7671 a         3718 c         4440 g         6704 t
ORIGIN      
        1 agaaggatat ttttttctag ttttttcgaa aaaaaggcgc tttctgattg ttgtattagt
       61 ggaaagggga aaaaaggagc atatttatca tgcaacaaaa ttacaatgag aaatctatac
      121 aacatcaatt tgatgcctac tgtaaaaaag tattacgaaa cgaaactaag tctattcgaa
      181 aacgaaacat taacataaca gataatgagg aaccattgac gaattcaaat gaaggtaagt
      241 actcccaaac tcattttgat gaacaagatg tctatttctt atttggaatg gaaatattaa
      301 tttcagatca aaagttatcg gcagccattg atcatttatc ggaaataaga agaaaggtta
      361 tcctacttta ttattttgca ggttttaatg atactgaaat cggtaaaatc ctcaatatga
      421 gtacaagtgg tatctggtat caacgaaaga aggcagttga acaattaaaa atggggtatg
      481 acttatggta gacaacccca aaaaacggca gtatccatta gttcctctat ccattatcca
      541 atctgctcta gaagaagatc caatttcaat caagatgata ctcaaacatt atcatagtta
      601 tatagctaag ttatgtttga caaatggatt taatgaggcg gggcaattta ttacctatgt
      661 agatgagtat atgctaagac agctagaaat taaattgata gaagcgatct taaaatttaa
      721 aattaactaa ctaaaaagtc ccctttccac ttattctgtt taaaagaatc ttctaatagt
      781 taaaggatta gtagattctt ttaaacagaa aacctattga tcattgaaat ttaaatacat
      841 tctatcagta cgttaacact tcaaggagca tattttagtt gcgccatgat ttcttactgt
      901 aaagaaggag cgataaacat tctaaaataa tttttatagt caataaaaag cgatgatttg
      961 aagttgggat aatgatactt ccgtaaggct cgccgtaaac ttggagagag gtgaaattcc
     1021 tatgtgcttt tgacaaaagc agctgattaa gaattatttc catatgttga tttcaacatt
     1081 ttttctatta tttttaagac caaattcagt gaggcaaata gcatcatgta aatttagttt
     1141 ttctattgaa cgttctccac tttttagttt ttttatagta ctcaaattta atcctgtatg
     1201 tttagagatc tcatttgcag tatggttttg taaagtttct cggattattg ttatattagc
     1261 aatttcatta cgcataatta tgttcctttc ttatcaaaaa tatcaatgac tacagtgggc
     1321 ttttagtgaa aaaaaggggt actacagtct cttttgaaaa caaaaaaata cagtatatac
     1381 gttttctaaa tgctaagcac caacgtttac actgtattta agggtttggc acccaatggg
     1441 ctgtgagggg ctcgaacccg cgacccgatg attaagagtc atctgctcta ccaactgagc
     1501 taacagccca aattaatact ttgttattct aacacctaaa ataaaaaagt gcaatactta
     1561 ttatcagaat tgagagtgaa ttttatggat tttcaagatt atgaaaaaat atttgaaagt
     1621 tatccatcga tattgaaaat tactgattta actaaaatgt taaatattgg aaaaaataaa
     1681 gcttataatt tgctgaaaga tagatcaata gaaaatataa aaattggagc aaggtacaaa
     1741 atacctaaaa aagctgtcat tgagtatata ttgaaaatac aagatgaagg taaaaatcat
     1801 gctataataa aatagtcaaa atttttgccg atatttcagt ttaagaggag agattgaatt
     1861 gaaattaaga gcaaacataa ttgaaaaaag aggaacatta caagtagttg tatcttataa
     1921 agactctaac ggaaaaatta gacaaaagtg gaaagataca aaattaaaaa agaaaggaaa
     1981 taaaaaacga gcagaaaaaa ttagagatga atttttacta gaattggaaa gtcaattagc
     2041 tatggaatgt gaatgtgcta atccaaattt attgttttat gactatttgc ttaattattt
     2101 ggaagtatca aaaaaacaag tagcatttaa cacatatgtt gggtataagc attacgtcca
     2161 taatcgaatt tacaagtttt tttatcctaa aaaaattaaa ctaaataggt taaagcctta
     2221 tcatttacaa gagttttatc aatatatgtt aaatgataac tgtacagcaa atacggttat
     2281 tcattatcac gcatttatac gcaaagctct tcaagaggct gttattacag aattgataag
     2341 tacaaatgta gcagataaag taagaaagcc aaaaaagaaa caatttgttt ctcaagttta
     2401 taatcaacaa gagatcttga aattacttga cattttgtca gaagaaaaac tctatttggt
     2461 agttctttta acagcatttt atggattgcg aagaagtgaa gttttaggat taaaatggtc
     2521 tgctattgat tttttaaaca aaaaaatcat gataaaccat gtaatcatag aaaatccaga
     2581 gaatatgtca cagttaataa agaaagatca aacaaagaac tcttccagct atcgtacatt
     2641 acctttggtt gaaacgattg aacgagcatt agtaaatcaa gctaaatggc aacaagataa
     2701 tcgtattctt ttaggagatg attatatttt agaagataat gagtacgtat ttactatgga
     2761 agatggacgg ttgatgaagc ctcaatatgt aacgcatcgt ttgagtaagt tgattaagaa
     2821 aaatggattg aaaaaaattc gctttcatga cttgcgtcat tctaatgctt caataatgtt
     2881 agatagtggc cagaatatga aatccattca agaatggctg ggacatgcat cgtattcgac
     2941 aactgctaat ttatacacgc atttaaccgc aggagtaaaa gatcgtgcag cagaagcatt
     3001 agaaaacgca tttgggttta aagaataaaa aaacaaaaaa caatatcttt ggggtatcgc
     3061 ttcttgtttt tagaattttt ttgttggcaa tttgttggca gtatgttggc aatgaaaaaa
     3121 ttcacaaact aaaaacctat ttataaagct ttataaaact tttttcacaa atagagcatt
     3181 tggttaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
     3241 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
     3301 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
     3361 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
     3421 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
     3481 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
     3541 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
     3601 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
     3661 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
     3721 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
     3781 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
     3841 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
     3901 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
     3961 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
     4021 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
     4081 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
     4141 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
     4201 atgttccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
     4261 ataaagatca ggtatataca gataaaattg acattacccc tgaagagatc tatcaacgtt
     4321 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
     4381 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
     4441 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
     4501 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
     4561 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
     4621 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
     4681 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
     4741 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
     4801 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
     4861 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
     4921 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
     4981 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
     5041 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
     5101 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
     5161 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
     5221 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
     5281 gctatgacgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
     5341 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
     5401 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
     5461 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
     5521 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
     5581 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
     5641 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
     5701 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
     5761 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
     5821 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
     5881 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
     5941 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
     6001 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
     6061 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
     6121 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
     6181 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
     6241 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
     6301 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
     6361 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
     6421 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
     6481 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
     6541 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
     6601 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
     6661 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
     6721 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
     6781 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
     6841 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
     6901 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
     6961 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
     7021 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
     7081 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
     7141 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
     7201 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
     7261 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
     7321 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
     7381 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
     7441 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
     7501 gtgattttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
     7561 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
     7621 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
     7681 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
     7741 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
     7801 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
     7861 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
     7921 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
     7981 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
     8041 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
     8101 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
     8161 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
     8221 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
     8281 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
     8341 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
     8401 tgaagaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
     8461 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
     8521 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
     8581 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
     8641 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
     8701 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
     8761 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
     8821 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
     8881 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
     8941 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
     9001 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
     9061 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
     9121 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
     9181 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
     9241 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
     9301 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
     9361 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
     9421 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
     9481 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
     9541 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
     9601 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
     9661 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
     9721 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
     9781 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
     9841 aaacaagcga taaagtggtc caaattgaga gcgtcaacgc tttcagcaat acgatcgtga
     9901 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
     9961 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
    10021 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
    10081 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
    10141 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
    10201 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
    10261 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
    10321 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
    10381 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
    10441 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
    10501 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
    10561 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
    10621 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
    10681 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
    10741 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
    10801 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
    10861 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
    10921 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
    10981 gccatgctga tcgtactgtc gctagcagcg tttctttgta aataatcatc acttaacaaa
    11041 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
    11101 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
    11161 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
    11221 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
    11281 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
    11341 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
    11401 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
    11461 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
    11521 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
    11581 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
    11641 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
    11701 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
    11761 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
    11821 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
    11881 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
    11941 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
    12001 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
    12061 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
    12121 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
    12181 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
    12241 cttatcggtt cagcggtttt tcaacatttg ttcagaatga ctgttgttca aggagtatgg
    12301 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
    12361 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
    12421 ttggttagtc ccgtcttgca agcgaattca ttagcaaatg cagaaatctt agcctcagga
    12481 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
    12541 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
    12601 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
    12661 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
    12721 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
    12781 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
    12841 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    12901 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    12961 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    13021 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    13081 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    13141 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    13201 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    13261 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    13321 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    13381 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    13441 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    13501 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    13561 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    13621 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    13681 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    13741 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    13801 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    13861 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    13921 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    13981 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    14041 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    14101 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    14161 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    14221 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    14281 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    14341 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    14401 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    14461 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    14521 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    14581 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    14641 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    14701 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    14761 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    14821 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    14881 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    14941 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    15001 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    15061 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    15121 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    15181 agtaattact gacgatctag gtttagaatt gaaagatgca acaatcgaaa acttaggaaa
    15241 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    15301 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    15361 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    15421 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    15481 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    15541 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    15601 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    15661 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    15721 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    15781 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    15841 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    15901 gggcggtatg atgtagtaat taagaaagaa ccctatttta acagggttct ttcttctttt
    15961 tttgttttat tttaagcaaa aggggcaaag aaggggcaaa aatatcaaag gctatttagt
    16021 ttatcaatta cttgttgttt ggctcttttt gttacatggt tataaataga cagagttgtg
    16081 tttgcatcag aatgccctac acgctccata atggctttaa gaggtacgcc taattcagat
    16141 aatagtgaaa cgtggctatg tctgaatata tgtgaggata gacttttttc taattccaac
    16201 tcttcctcta ctttatggag tatagcgtta aatgagtgca gcgcaagcgg agtgccactt
    16261 gtagatataa atatatattg atcgggatct gtgggtctac ctgcaagaat attgtcagct
    16321 atcacacttt caatcaattc ttttgcacga ttgggtaatt gcacttcacg ttgcgaataa
    16381 gtattttttg gagttgtttt tatagcatta tccattttca cagatgtgta atctaaggtc
    16441 ccattaatgg aaattttccc atcctcatag tccttcattt gcaaagctag caattcccca
    16501 tatctcaaac cagttaaata tagaaactca gctattatgc cgtgtagttt tctgcgagga
    16561 ttggagtata gctgtttcag tatttgatca atttcttctt tctccagata ttttttattc
    16621 atagaaagcc ttcttttttc ttcttccact tttttaggat ggattttaac tgctagcgca
    16681 gggtttcttt gaatgtattt tctatcgatt gcatagttta gcataacaga tagagttgtt
    16741 tttgtttgtt ttgtgtagtt cagtgagagg tcaccaaacg tatacatatc ttcaattatc
    16801 ttattaatga gtgtctcatc aatgtttcta acgatcgtat catcgcttat gtgcttagaa
    16861 acatgtttca tcatcattgg aaccttcaaa tagctagtac gtttaacatg ctgcttataa
    16921 tattcatacc attctttata cagctcacca aaagtgatat ctgatttatt gtaatcttcg
    16981 agtgcttctt taattttttt atctagaatt ttctgagctt ttttccacgc ttgtggtgaa
    17041 ttacttgtaa gtgttgtaga tttttttcgt gttttttctg tataaggatc tacatatctt
    17101 tcaataaact tgaattggcc atctttggtc tgttcaaccc acacttttaa catctcctat
    17161 catttgctat aataggcata acaaatagac ctatataggt ttgttttcta aaagcacgct
    17221 cttactctgg acggtggggc gtgtttttta tttattatac agttgcttta aaaatagctg
    17281 ctttttcaaa tgatactaaa ggagagaagt ggatttctat ttctcccttt tggtttaacg
    17341 tgaaatgagc agttacatcc atttgttttc ctggagcaac agatcccatt gtattttcgt
    17401 tggcatatgt ctctgatttt ttgtcatctg gtccatatac ttccacatct gtacctacag
    17461 ggatatctga atcgccatca ttttttacgg tataagtaat ttttactact tgtgcaggtt
    17521 ggttttcttc aaattgattt ctttcatcag ttagttctac actatttagt gtatattcag
    17581 catcaccaac ttttacagtg tcaccaatct tatagaaggt atcgcttttt tcttctttag
    17641 aagatgaagc agaggttgaa gatttagtta ctttttcgcc accattatca cttgctttat
    17701 tactatctga tccaccattt aaagcagaac caataattat aattaaaatt actgctaata
    17761 cccaaaacca tacttttttg taaaaaggtt tactaacttt atacattttt ccgtcttgac
    17821 ccataacttt ttttgccatt taaatattcc tcacttcttg ttataatata tttgcgatct
    17881 cagaaatgag gtatgagtcc gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg
    17941 gtttttttaa taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct
    18001 taaaatagca ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat
    18061 actccataaa tttttcgaga ttaaactgag attcatcagt cagttcgttc tcaatataga
    18121 tatttaataa aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag
    18181 ttgtagaagt atcgtacaaa actgaatatt caaaatgaga agcggtgaaa tgcgcaagtt
    18241 catgagataa atgaaaggct tctgcagttt cgccatatag attttcattc aaaaaaatga
    18301 ttctaggctt cggataatag aagccaggct ctttcatttc catgtaaact actttcaaat
    18361 tgtattcgct caacatttct ttcaatttca aatacataca acccatcact ccaactattc
    18421 attttcctct aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc
    18481 ttttccatca aaagaaaaaa cagtatcgtt ttctgataaa tccacatgtt taggggtttc
    18541 tctttcttct ctacctagaa ggtagtctac agagactcca aagtaatcag ctaaccgagc
    18601 taactcaata gaattaggag tttgtctttt ccaacttcct aaatatccat tcgaataccc
    18661 gaaattcatt tcgagctgtc taatggataa tcctttttct tttgctaact cctttattat
    18721 ttcgtaagta ttcattgata aatcaacctt tctgaatgct tacaaaaaaa gtttagataa
    18781 ataatgtaaa aatagttgac ttatatacat tattaatcta tactatgttt tgtaaacaag
    18841 tttaacaact aaaaagacaa caaaaaatac tattgattaa taaatgccaa ccgccaagaa
    18901 agctttttaa atcaaagttt atatgtctta tttaactatg catttagtat agattattaa
    18961 tctaataaag tcaacgcagt tttttaaaaa gttgttaaat ttgtttacga atataaaaga
    19021 aaggagagaa gaatatggaa aaaacagtct cggcaaaaat cgaagatttg aaattagaca
    19081 tactgaaaca agcaaaagtg gcgatggaac acgcggtaga taaagaagac tctgccatgg
    19141 ttgcagccat agcagagatt ttagctcacg tttagtcatc gtttttacgg tcttctggaa
    19201 gctgaccata gataacagca taatgaatat ctaaataagc ttgaacaatt tcttttggcg
    19261 aaattgattc gcctttagta gatacttgtg attcgtgatt tagccatgca acaactaaat
    19321 ccgcagcgat ttcaaacggc gttacacttt gtgtcagttg ccataaagaa tttcacaata
    19381 tgtatggtcg tggtaataat accaaagagc aatttgagga atttatggaa attaaaaaat
    19441 acaaaaaatc atttattaaa ggaggctaaa caatgaacaa ggaaatgagc caaacaatca
    19501 aggtacaaaa aatgatagat gatctaacac atggaattga tagccaagcc gataaaatca
    19561 tcaaggaatt gcaaggacaa aaagttaagg atgcaaaaat gcttctcaag actatcaact
    19621 ttgaaatgaa tccaacaaaa agaaagctcg ccgatgtgtt ggaagaaaag ttggcttccg
    19681 ctatcaacga gcaagaatta ctatttgaaa ccgatacttt taacagctga tgttttatag
    19741 atgacagttt catccgaatc tatatcatcg ccgagagaaa agaatgttgc ttttgacaaa
    19801 aagccgataa gacctacaag cggtcttgat gttgcaagag tagatacccc aatatcacct
    19861 tgcaaggtat tcacgtaaat ttgagaagaa tagaacattc catctttttc ttcgatattt
    19921 gacgcatcat cgtatccaac gaccaaagta tcggcaaata ttttgatttt ttctccatca
    19981 agcatagtaa ttacaaattc tggtttcata aattttcacc tccttaacaa ttatttcagc
    20041 ctgtcacact gataaggaaa ttataccaaa gaaaggaatg agaaatatga atacaccaca
    20101 aatttttaat ttcgagcaaa acgaagttcg aactatttta gtaaatgatg aaccatattt
    20161 tgtaggaaaa gatgtagcag acgttctagg atattcgaat ccgcaaaaag ctattcgaga
    20221 tcatgtggac ttggaggata agacgcagaa cgattcgttc accgtcaatg gaacagcagt
    20281 tgttttgatt aacgagtcag gcctttacag tttaatctta aaatcaaaac ttccctctgc
    20341 caaaaaattt aaacgttggg taacgagtga agtccttcca acaattagaa aaacaggtag
    20401 ctattcaaac gtacctcaaa gttttgcaca agcattgcgt ttagcagcgg atttagaaga
    20461 aaagaaccaa ttactcgaac aacaaattgc cgagtacgaa ccaaagatta gctacttaga
    20521 tacgattctt tcatcgacag atacggtagc tacttctcaa attgcagctg attacggaat
    20581 gtcggcaatt gctctaaaca aattgctaaa cgagttaggt gttcagcata aagttagcgg
    20641 acaatggata ctttaccgaa aacatatgaa ccaaggatac acaaaatcgc acacaagtga
    20701 gataccgaaa gccgatggcg gcactaaagt tgtaatgaat accaaatgga cacagaaagg
    20761 gcgagtgttt atttacaact tattaatcgc agagggctat taccctcaaa tggatttaga
    20821 ggaaattggt tagaaaggag ttttagtatg actgacattg cagaaatcac tcaacgagat
    20881 agagaaaaaa tcaaagaata tgtcgaaagt tcaaagttct taacttacac catgcttgct
    20941 gaaagatttg gaattagcaa aagctactta tctttaattt taaacggtaa aaagacttct
    21001 gcagaagcaa acagaattat agattcgatt atcactatgt acgaattgta gagggaggta
    21061 aagtcgttgg aagataaaat cattcttaca aaatcagagc ttcaagaaat gttaaacatc
    21121 gagtatggaa cgataatttt taggagggat aataagtgaa aaaaccaaca ctttcagagc
    21181 tgatagaagc tgctgagaag gcagtaaaac cagacgactg gtaccgacaa agtttaatct
    21241 tggagaagtt ccacggcatg tcaaaaacta ctttagttga atactgcaag gaaatggaaa
    21301 caattcctga atttgcagaa ggaatacttc gtccaggtca ctcaaccaca tttattcatt
    21361 accatacttt tatttggttt ttaaagtgga aagacgcaaa caaatatcgt gtaaaaagtt
    21421 tatcgccttc agatgttttg aaggaggcaa gttaatgggc aaattcaaca gagcactagt
    21481 attcagcgca ccgctaatca tctacgcttt aggcctctgg ggaagcagac aagcgttgat
    21541 agggacaatc gtttacatgg tttggatttt tatggggctt gatgaagctg agcacagagc
    21601 gaaaaaagcc agtcgggagg gactgactaa aatatgattt ttttattttt gttcctttgg
    21661 gctgctattg cgtacttcat ccagctcctt gttgtcaaag ttgctcttat ttcttttagg
    21721 aagaacaaaa gaaaatagcg ctataaatga aagataagta gccgctacat taaactgttc
    21781 ttttgaaaaa gttacagtca tcggaatgat aattgatatt gaggaagtaa tcttaattaa
    21841 aaatatagca atattttcaa catcgcgttg gtttttattt aacattgtcc aacatgtttt
    21901 agcaacaata aaaataatta cacatgatat tagcacacag actgaaaaaa gttttagaaa
    21961 tgcgatgtag tcagtgttat tgaactcctt caaaatacta tttttattca atgtaataaa
    22021 aggaattttt ttaattgaag acaggaaaga taaggtaata aacagaatta tatatatggt
    22081 taaagtcgca gtaagaatgt aattgagctt tttcctcata gaatcacctc actttctaac
    22141 gaaaattata ccaaaaagga gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa
    22201 agaagctgta actacgagct tacaagtggc agaaagtttc aaaaagaaac ataagcatgt
    22261 actagaagca attgaatcaa taaaaagatc ggtcgaaaat tcggccaatg ttgaagatgg
    22321 gtccaatttt ggacagatgt ttgtggaagg gaacgagcca gactcatacg gaagaagtcg
    22381 gagagtttat ttcatgaata gagacggatt ttccttgcta gctatgggat tcactggaag
    22441 taaagcaata aatttcaaac taaaatttat tgaagctttc aacgaaatgg aagatgttat
    22501 tcggaagaat actgttcctc aaacaattga aga
//
LOCUS       DASJPC010000037        22332 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJPC010000037 DASJPC010000000
VERSION     DASJPC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.037"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..296
                     /locus_tag="U4A13_001433"
     CDS             <1..296
                     /locus_tag="U4A13_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1779733.1"
                     /translation="DCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRN
                     GLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(367..1677)
                     /locus_tag="U4A13_001434"
     CDS             complement(367..1677)
                     /locus_tag="U4A13_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1779734.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1689..3107)
                     /locus_tag="U4A13_001435"
     CDS             complement(1689..3107)
                     /locus_tag="U4A13_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1779735.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3347..4915)
                     /locus_tag="U4A13_001436"
     CDS             complement(3347..4915)
                     /locus_tag="U4A13_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN1779736.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4919..5200)
                     /locus_tag="U4A13_001437"
     CDS             complement(4919..5200)
                     /locus_tag="U4A13_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN1779737.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5197..6120)
                     /locus_tag="U4A13_001438"
     CDS             complement(5197..6120)
                     /locus_tag="U4A13_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN1779738.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6117..6995)
                     /locus_tag="U4A13_001439"
     CDS             complement(6117..6995)
                     /locus_tag="U4A13_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN1779739.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6982..8319)
                     /locus_tag="U4A13_001440"
     CDS             complement(6982..8319)
                     /locus_tag="U4A13_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN1779740.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8322..9425)
                     /locus_tag="U4A13_001441"
     CDS             complement(8322..9425)
                     /locus_tag="U4A13_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN1779741.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9499..11007)
                     /locus_tag="U4A13_001442"
     CDS             complement(9499..11007)
                     /locus_tag="U4A13_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1779742.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10973..11665)
                     /locus_tag="U4A13_001443"
     CDS             complement(10973..11665)
                     /locus_tag="U4A13_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1779743.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11687..12661)
                     /locus_tag="U4A13_001444"
     CDS             complement(11687..12661)
                     /locus_tag="U4A13_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1779744.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12961..13833
                     /locus_tag="U4A13_001445"
     CDS             12961..13833
                     /locus_tag="U4A13_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1779745.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14415..16613)
                     /locus_tag="U4A13_001446"
     CDS             complement(14415..16613)
                     /locus_tag="U4A13_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779746.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16845..17837
                     /locus_tag="U4A13_001447"
     CDS             16845..17837
                     /locus_tag="U4A13_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1779747.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17897..19318
                     /locus_tag="U4A13_001448"
     CDS             17897..19318
                     /locus_tag="U4A13_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN1779748.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19311..20810
                     /locus_tag="U4A13_001449"
     CDS             19311..20810
                     /locus_tag="U4A13_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN1779749.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20849..22243
                     /gene="uxaC"
                     /locus_tag="U4A13_001450"
     CDS             20849..22243
                     /gene="uxaC"
                     /locus_tag="U4A13_001450"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1779750.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6778 a         4549 c         3873 g         7132 t
ORIGIN      
        1 ttgactgtgg aaaagaattt tctaactgga aagccattag taaccaacat gatattgata
       61 tatattttgc ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga
      121 ttctgcgtcg taatggactg ccgaaatcaa tggattttag aaaagtgaat cagacattta
      181 tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac
      241 caatcgaaat atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa
      301 tcatatatca ttaaaaaaaa taaaggtcaa gctaaaatat tatttaagct tgaccttatt
      361 ataatttcaa cgattgtcaa aagtattgtt tgcagctgtg ttgccagttt ctatagataa
      421 atcacagctt ttgaaacata agttttgtac tgcttcgatt cgcccgaatt cttttgcagt
      481 tatttggcaa tgagaaaagc agacatcttc tataggcttt tcaggaaatg ctactaaatc
      541 aaaagctcgg gaaggaagtg agtaacctgg agaaaggcat gcctgtacgt ttttgatgtg
      601 tatgtctttg actttcgtca tttgctttga ttcaggtatt tcttcagcca ctgcttgcca
      661 actttctggc atttgagcaa ggtcattgct attttttcta ttgtattcat tatgccagtc
      721 cattatccaa gaaaaaggaa attgtacatc aatcatattc agattttcta ccctgatatt
      781 ttcgatcact ccccctcttt cttttgaaga tttcattcga ataccacagt ctgactgaaa
      841 aaaattgata tcgtgaatat atacgtcact aactccagca ctaacttcgc taccaatagt
      901 tacaccgtat cctgaacgga ttttacatct agcaacctcg attcttgatg attttcgatt
      961 gactcttgca ccattgccat ctctgccaga cttaatagca atacaatcat caccacaggc
     1021 caaatcacat tcatagacac gcacatttgt tgaagaatca atatcaatcc catctgtact
     1081 tggtccatca ttgttgcgga tgattacttt ttctactaat acatcgttag aataagtgat
     1141 ttgcaagttc caaaaacctg atttctgaaa aatcaaatca cgaacaacaa catgttctgc
     1201 atgatacagt aaacaagcgc gtggacgttt aatagcataa tccgcaatcc aacgaagtcc
     1261 cttttgatcg tattcagctc ttgtcccgct tttttgatct tttccccaat acagctccca
     1321 ccaatggtct ccttgaccgt cgattattcc ttttccttct ataagaatat ctttcgccga
     1381 gagaacattc aaaatcgctg ctggccattt catttctacg ccggctactc gtgtatcgat
     1441 ttctggaaat gcttcgatat ctgttctccc cttgaggact gcaccttctt caaagataag
     1501 atgactctta tcttttaaaa aaagggctcc gactaaataa atgccttttg gaataaatac
     1561 aggtaagtgt tctttcgcac cttgatcgat tgctttttgc agtattattg tatcatcttg
     1621 ttgagtgtcg tttgttttaa agttcaaagc attgataatc cctttttttg tccgcaatac
     1681 gtttctccct atctttcatt gatttttttc aattcttcac caataaatac agtttttgaa
     1741 cgattcagtg ggtaacctac tagcaaaatg attgcggata caaataagca gaatgctggt
     1801 aacaagtttg ccatcgtata aatcctttct tgtactaaaa ctgattggat ggctccacct
     1861 gttgtagacg attggtaacc aatgagtgcc agcataaatc cgcctaccgc tcctgcacat
     1921 gcttgtccta cttttctcgc aaaagaattt actccatata ctgttccatc ttctcttaag
     1981 cctgtcttat attgatgata atcaatcacg tcagtaatga atgcccagac catcaaattg
     2041 aataaaccag atcctaaagt agcgataaat aaaagaatca gatataccca tgcatttgtt
     2101 atatgaagga aatacatcag caagtacata catgaagaag caagtaacgc aaatacgctt
     2161 gcttcctttt taccaaataa ttttgtcagc tttgtagcaa atggtgctaa aactactact
     2221 gttccgtaag taaataaaag agcaacagac attgcttgtt tattatggaa gtaatcattg
     2281 aataaataag tcatattcgt cccagctaaa atctgattga tcacaatgaa aatgtcaaca
     2341 atgactaaaa taatcagtgc tcgattagct aacaaacctt ttaccaactg agaagcagat
     2401 actttttcgt ttttgacaac tcttactctt tcagtagtca atttacaagt taacatatat
     2461 gcgacaaatg ctattgcagc acatccgatt gaaatccaaa agaaatgatt ccctgaaacg
     2521 acttgttgtc cactcttgtt atgggtatac attaaaatcg gaatgaagaa tcctgtagtt
     2581 gatccgccaa tcgcagaacc aatgcttcgg aacgtggaca acgaggtgcg gtcattagga
     2641 tttgagctaa ttgctgctgc cattgctcca tacgggatat tgattgttga gagaaaaatc
     2701 ccataaacaa tgtatgaaac aaaaatataa gcaatcttca ccgtcattgg aagatgttgg
     2761 acgaatggca aaaacaaaat gacagtcact aagcaaaatg gatacttcat ttttctgatc
     2821 caaggaataa atcggccagt ttctgttaac tctgagacat cacataatct tccaaccatc
     2881 acatcagcaa acgcatccaa aattctcgaa atcaagaata acgtacctac gattgcccct
     2941 gaaacgccaa gaacattcgt ataataaatc atcaagaaac tattgactaa acctaaaata
     3001 aaacagttac ctagatcacc gaacatgtag cctatcttat cggaaatccc aaattccttt
     3061 ttttcttttt ttactgtatt tgctgtaaca gcattttgtg aaagcatttg aattcctcct
     3121 acctcttcta gtaaacgttt tcaaaattat aactcttttt ttcttgtttt tatattcaca
     3181 atgttggctt tttattgcat tatcttgaca gataaaaaac aaaaaataat ctaaaaacat
     3241 tgatgcataa gattgataac tttttttaca taacaatata tacaaacaaa aaaattcact
     3301 aactagtgaa aataagttag tgaacctcat ttattaaaga attaaattac agatgatctt
     3361 ttccatcata cttgaaaaac tcaaaatcag cataacttcc tcttttttta tttagatcat
     3421 gcactgctat cccgataaaa tttccagtaa accctcctga gagtatacac atgttttttt
     3481 gcggacctac tttcttccat tcttcagttg atacgtcttt caaatgaaac tgtgctgtct
     3541 ctttctttac agtgactttc aaaaaagtct tcttttctgt gagtggaatc gtccaatctt
     3601 ctatttgtgc tactccgttt tctgattcta tcatacgtag gcattttccc ttgtcctcat
     3661 tccaagtaac ataacaataa atataattgg attcatttaa gtacagtacc agtccagcca
     3721 tctgattaaa attattaggc atgtattcaa atgctgtctc cgcatcaaaa gtaaaatccg
     3781 tttgccgaat agctaacaaa tgatgttcga aggtagattg aagagattca ccggaaatca
     3841 gacgtaaaaa acctggtcta cattttgtat cacaccaatc agcatttggc aaaatgcgtc
     3901 tggcattcca gttcttcttt agctctcctt caaatgtatc cataaactga tgatcttttt
     3961 cttgttgtac ctcttcattt gtctcaatca ttatttcttc catcggagtt gtctggctat
     4021 ctttaagtct taaccaatcg tcggacgtcc aatacacttc ttgaatagcg gtttctcttc
     4081 ccaaaatagc cgcaccgttt aatggtctgg tacaaagata tgtcatgtac catcttcctg
     4141 acggggtttg tactaagctc ccatgtcctg tacattgcaa tgtagaagca ggtttgtcac
     4201 tagctgtcag cataggaaaa ccaggatcaa gctcgtaagg accagtaata ttctttgctc
     4261 ggcaaactgt aactgaatgt cctcttcctg taccgccttc tgccgtcatc aaataataat
     4321 acccgttacg aaaatacaaa tgtggtgcct ccgttttcgc taattctgta cctgcaaata
     4381 ttttatagat gggaccatca agcttcttct tctctgcatc atattcttgt aatacgatac
     4441 cgctggattt attcggtgtg tccagacgat aatcccatag ttcattgaca agccattttt
     4501 ttcccgtttc aagatcatgg aacaaagaag gatcaaagcc gttactattt aaataaattg
     4561 gttcggacca aggacctgtg atctcagatg cagtgatcaa ataattgtgg acatctttaa
     4621 acggacggcc tgcacttttg acatctgtgt aaacaagata aaacaatcca tcagcataac
     4681 tgatctgtgg cgcccatata cttccatttg taggatttcc ttgcaaagat acttgagtgg
     4741 tcaaaatatc cgttgtatgt gaccagttga ctaaattttt cgactcatat actcggattc
     4801 ctggaagcca ttcaaaagag gagacaataa tgtaataccg ctctttcact cttaatatat
     4861 taggatctgg attgaatcct cttaaaatag gatttttcac tttgatagtc tgcatgattc
     4921 aggctccttt tctcattctt actcgatata aactgaatgc cccagctgct aaaaaagcaa
     4981 gtgaaataaa caacacaacg gcaaacagac cagtcatgtc ctgtaataat ccaaatgtat
     5041 tttctaaaga caaagttgaa tgatctacag caagcgactg ataacttttt ttaaactctg
     5101 acgcagacat attacttaaa gtaactgcta acccgccaaa gaaaatcaaa gatagactgc
     5161 caccgaccat tcctagcaat agttcccatt ttcttttcat ttctgaaccc cctgcttttc
     5221 taaatgtatc cattcaggtc ttttgatcga tgtttcataa ttgttattac gttcagagaa
     5281 tttgacagtc tgttcatttt tcttgtcatc ccaattatcc caaccagttg ggtgaacatg
     5341 cccacctatc ttacacgctt caaataagac atttgcatac ggacgccacg gtcttcctaa
     5401 atagacgttt ctagtatcag gttctgcagt caaggtacag ctttcaaaac gataccctgt
     5461 tttttgattt ttcggcgtag atgctgctgt gatgtatcca ggatttttac tggctaagct
     5521 atggatatga cactgatgaa aaacggcatc tgccccacca aatatgaaat ccaccgttcc
     5581 ttcaatccag caatattcat aggtctgacg acaatactca ggattggcag aagccggtgt
     5641 acaaaaagga gtaccatctt tttgcaaatc aggcaacgga cccgtgcata aggtatcctg
     5701 ataaccagat agtcgacagt tttttaaaga tacgcgatct cctgaattga ataaagcaac
     5761 agcctgtcct accttttctc ctggacctgc tgtattttcg atgtgaagat tttctaatac
     5821 gacattcgat ccttctaaga aaaaagtagc acttcggaaa gtccctaact ggctaccgtc
     5881 aagatactcc tgttttgcaa aacgattacc ggagataact actgttcctg ctcccacaag
     5941 tttaaaattg gataatctgc tttctatata ttcatcatat cttccttctt ttactatcaa
     6001 gacctttgga tggctatccg gcaggcctgc caaataatcg attccttctt ggatagtttc
     6061 aaaatccgtc ggggtatcta tagcattctt tccaattatc agtttgtctt tcatcctcat
     6121 aaatgctccc ttatgaattg tttccaaata tttctcattt cttgtgtttc catatgaccg
     6181 cctgttaaag aaaaagaaga aaagttttct ggactaccca ttgattgata ctttttgtcc
     6241 agcccttttc tcagcttcag tacgccttcg tccaagcaca tcgtatcgtt ttttccaact
     6301 aagctaagac gagggcgcgg agcgattttt tcttggattg ctaacgttgt ataagctgtc
     6361 aatagatttg gcacatagta ataaaaccca tgatgatcta ggcggcgatg tagcaaaagt
     6421 gtttcgatgt ccacctgtgc cgctagatca atacaaactt tgacacgaga atccaatgct
     6481 gtcacccacc agcttaataa gcctcccata gaaagaccaa tcgttgcgag ccgttgagta
     6541 tctgtgtctt cccttgtctc caaataatcg atcaagctga tcacatcata aatacgcatt
     6601 ccccataacg tttttcctgt taataaaaat tgtttgaaca actcgctttc agaaatacct
     6661 ccgcgttctt caaatcccca agcatcaatc gaccaaacag catatcccat tcctgtcagt
     6721 acttctgcaa aagatggaga agctaagtaa ggagcaccat ggatcaattc acttttcccg
     6781 actgaaaaat ctccgccgtg agaatgcagg taaaggaccg tcggaaacgg tccttctttt
     6841 tctggataag catagagggc tggtactgtt tccaattcat ttaagtacaa ttgatagtat
     6901 tcaagcataa aaccgttttg tttttctcgg tataatcgtt ttccactagg ggtatgtctg
     6961 ctagatggag cacctaaaaa atcattcaac tgcataagca aagtttttac gaacaaccag
     7021 ttcatccata ttttctgatt gatttgattg gaatttctca atactgattt ctttagtatt
     7081 ttctaataga aatgctggtc cctcgtgatt ttcaatcgac atctggttaa aggaaacctt
     7141 acccgcaaag ccaatataaa atccttgtct gctcatttcc tctattcctg tcatcatagc
     7201 aggaacaccg ggaactgcat cttgggccat cgagacatcg atattgtcaa atgtgatttc
     7261 ttgtatgaat tgttccgcta atccataaat aaaacctgca gaagcatgga cattccttgc
     7321 cgtgatattc gaaaaatgga tccttctgaa tgctgatgtc cgttcatcta tcggataagc
     7381 ctttttttcc catacatacg gttctttccc tcgcggacca cagaaataat aaagattcag
     7441 tataaatgga cacatcacat tatccatgac tatattggat actcgaatat cttcaacgat
     7501 cccgcctcga cctcgtctag atttcaatcg tattcctcga tcagtttctt ggaagataca
     7561 atttgaaatg gtaatattac gaatactacc actcatttca cttcctaata cgacaccgcc
     7621 atgtccatgg accatcgtgc aatttgtgat cgtgatattt tcgcaagcga tcctttcgta
     7681 cgtatcttca gtccctgcct tgattgcaat acagtcatct cccacgtcaa tatggcaatt
     7741 actaatacgc acatttttgc atgattctgg atcaattccg tctgtatttg gtgaatccgc
     7801 tgggttcaag atcgtcaagt tatcaaacgt tgcattactg caaagaatcg ggttgatcgt
     7861 ccaacttggt gactggatca atttaatatc tttcacagta attcgatgac agttatggaa
     7921 actcatcaat ttaggacgag gataagcgag attatctggt tcatttctaa aagtatgcca
     7981 ccattttttt ccatttccat ctaatgtacc gaaaccagtg acagagatgt tctctacatt
     8041 ttgagcatag atacaggaag cataaacttt tcgatggact ccttcccaac gtgaatgaac
     8101 aacaggataa tcttttggat cgtcagagaa tttcagaacc gctcctgcgc ttaaatgaag
     8161 ttcgacattg ctttttaaaa ataaagctcc tgttagaaat tcacctgctg gaacaactac
     8221 ggtcccgcct ccgtcacttg ctgcagcatc aatggcttgt tgtattgctt cagtattcaa
     8281 ttcatcaatt gatgcgccaa atgtcaaaat atcatacata tttataatgc ctccacttca
     8341 atcaatgctt gcaagaatgc accaacgcct ttttgatcat tacaaataat tggttcactg
     8401 atgtagtaag catatgaacc atctcgctga tctgctccgc ctaaccctgc tacttgacag
     8461 tttttattca agttcagcca gccttctttt gtttccagaa caaattcatc aagtaatcct
     8521 tgatgtgatt tcttcacttg tggaagccat tcttctttgt tcagcagtcc caaacgtatt
     8581 cctttagcca ttgcgtaaac aaacatacta ctgccggaag cttccagata atttcctttc
     8641 cgttttgttt ctgttgttac ttgataccat gtcccgctgt tttcttcttg atatggaagt
     8701 aatgcagcca gtgtcttttc tagaatttct ttcagcaaag aacaatctgt ttcttcatga
     8761 agtaattcat aggtatctgc tgcagccatc aaataccagc caatcgatcg gctccaaaaa
     8821 tgatgggata gacctgtttt tggatcagcc catggttgga ccttcttttc atcccatgca
     8881 tgaatcagca aatttgtttt tggatcgaat agatgtttat aactgatttc aaactgtctc
     8941 aaaacatctt ggtaatcttt ttcttcacta aaattctgga taaattcagc ataaaaagga
     9001 gaacccatat ataagccatc caaccaaatt tgataaggat aaatttcttt atgccaaaaa
     9061 gctccttcgg atgtgcgtgg atggtttgcc aactgctggg aaaggagttc tgctgctttt
     9121 ttatattttt cttcgcctgt ttctttgtac aataaaaaga aaagctttcc tgtatttaca
     9181 tggtcgatat tgtattcttc aacgctgtag ccacggatcg ttccatcttc ttgcacgaaa
     9241 tagtcgatat tgtcttttat gaaatcaaaa taaatttttt cttgggttcg ttcccaaagt
     9301 aattggaacc ctttaagtac gacgccatag tcataagacc attttccttt ataccacttt
     9361 tcttcatata agcgaggtgt tctttccatg atactttttg ctaatttctt tgaccagttt
     9421 tccattattt gctctccttt aaaaattaaa ataggatttg ggacaaatac caagaagtat
     9481 ttgtcccaat cgtctatatt actgtgccgc tgctttgtat gcttcctcgt actcttgtgt
     9541 gattcgatcg cctccttgtg ctttccagtc ttctacagct tttttgaatc catcttcgtc
     9601 aagctgtccc atgatgtatt gcaccgtagc atcaatgacg attttttcaa tttctgatcc
     9661 tacttcattg ttcgtcggtg aatccaatgg cacagaagga tccattacag caaattcttc
     9721 attttctttg atcaattgat tagagaattc tttttcaggg tctgtatctt tgaaagagaa
     9781 cgtcacttca ctaggacgag agcttgataa cggttgcacc tcttgttgcc aaagatccat
     9841 atcgataaat tcgatagaac catcatcatt caattcataa tgcgttcctt cgataccgcc
     9901 agtcatcaat ttatacacat cttcttctaa aagatcatca acaaattgaa gtaatttctt
     9961 caattgtgct tcgtctttta cttgagattt gggaaatgct agcaagccgc caataccatt
    10021 tccttctgac caaacatgat aaccgccgcc ttcaccatct gagatcttgt ttacaggcac
    10081 taattccata tcatcttgaa taccttgacc catcgtcttc agattcttga tatccaccat
    10141 tccagtgtag attccagttc ttccttgtgc aaattgttct tgctgatcag tcttttgggt
    10201 aactgcaaag tcttgtgcca aatagccatt ctcaaataga tctcttgaaa aattcaaagc
    10261 tttcatatac tcttctgtat caaattctgg agtgaatttg ccattatcgt ctacagccca
    10321 gccatttggt gctccaaaat aagaagtgaa aagtttaaag ctggtataac gaatatcact
    10381 aggcgcacga tcgccaaaac caactgtgtc gtccacacca tttccatcag gatcgttttc
    10441 tgtaaattgt ttagctactt cataaagctc atccatggta gttggaacat ctagtcctag
    10501 attatccaac cagtcttttc gaatgactaa gccagcacga gcgtagtctt tttgtatcgg
    10561 tacgccgtac aaggcaccgt cgatactcgc agcttcgatt ctatcttcag aaatttgact
    10621 taaattttta tagtctttta aatattctga tacatcccag aatacgcctg attttaacgc
    10681 attgcggaca gaagagtttt tcaacatcgc aatttgaagt gtaacgatat ctcctagttc
    10741 atcagaagcc aatgcagttg ttaatctttc ttcttttgaa gcatcaggaa tccagttgaa
    10801 tgtcagttcc gtatcggttt tttcttcgat caagttcaca atcgtgtctg taggcggtga
    10861 agcatgatgc aaaatattta accaagtgat tttagctttt ccatcttctg tctctgaaga
    10921 actgttactt gaactgctgc cgcatgcggc aagtgctaga attcctgcaa aactaagacc
    10981 taattttacg agcgttgctt ttttcataaa ttttctcctt cactgtttta ataatatttt
    11041 tgttttcttc tgttttttct gtatatgagt tcttccattc caaatggcaa aatatagaat
    11101 ttgctgtcca cgtgactgcg gaaaagaaga gactgccacc gataaaggtt aaaacacctg
    11161 gcattgtttg gatgatcatc agataaatcc ctaccatact taatatcaac actaatgagt
    11221 aatgaagatg tgagaatccg aataaaaatg ccattttcat tttgaaaaaa tatcctttcc
    11281 aatcataatg agccatgatt gggaaaatat agagtgctga gagcacatat atgaacgcag
    11341 caatatacaa agcgatccta agataccaat tgtgtatgaa agaaagatcc acaagtaaaa
    11401 caatcccgat ggcgccatat atccagctca tcagcaaggt ttcacgatag ttttctttaa
    11461 aatatgtgaa gtacgttttc gtcaaaggaa acgtttcttt tgagcgcagc cattttctaa
    11521 tcactgcaac catcgcatac gatgcaggac cgaaagtaaa aactcccaaa cctgcgatga
    11581 ctaaaccgcc ccacagaaaa ttcaaataaa ccaactgtaa aatacgattt agccttccgt
    11641 ttattttatc taatgactgc agcatatctt tccctctctt tctatcttag taaacgccgt
    11701 cttctcctag ctttttggca atcttattag ctaaaattac cataaacaat cctacgaccc
    11761 ctttaaacaa tcccattgct gtactgaaac ttaactgtcc attcttcaat ccagctgtat
    11821 aaatataggt gtcgaaaatt tctgcaacgc ttcgattcaa tgaattcaat aacaagtaca
    11881 tatgctcgaa ccctaagtct aatgtctgac cgatttttag aatcagcaag gtaatgatta
    11941 ctggacgaat tgccggcaat gtaacatgcc atgttttgcg taagcgtcct gctccatcca
    12001 tttctgcagc ttcatacaac tgtgtatcga ctgcagttat cgctgaaagg tagatgatcg
    12061 tagaccagcc caactctttc cagatcactt gtccgatata tactgtgcgt gtccattcag
    12121 gggatgttag aaaactgatt ttttcaaatc ccattgttgc aaggaattca ttcaataccc
    12181 ctccgtcaac attcaaaaat acataagtaa tcgaaacaat gattacccaa gacatgaagt
    12241 gagggatgta aatgatcgtt tgaatcccgt ttttcaatcg tttgttcgtt acttcattta
    12301 atagtaaaga caaaatgatt ggcatcggaa aaaacacgaa gatatttaat ccgaacaaga
    12361 tcaacgtatt tcttaataat gtaaagaatg tcggttctgt aaacaaacga atgaaatgtt
    12421 tgaaaccgac ccaaggactt cccattattc ctaaaaatgg ttgataatct tgaaaagcaa
    12481 taacgagtcc ccccatagga agatacctaa aaatcaagaa ataaaggact cccggcagaa
    12541 tcatcacata taaaaattta ttcgttttga ttcctgcaat tatttttttt cgtcttctga
    12601 gcttttcttc ttttttcgat agcgtcagtt gaaatggttc ttgtttcttt acactttcca
    12661 taagcccctc cttagttttg tgaatgtttg ctgcttatga cttcatgata gataactatc
    12721 tgaattacgt atataatcat ttttatcgta agtaaaaacg cttacatagc aatgattttt
    12781 gtgctataca aaacgataaa gtgtattttt tcacaaataa actacgcata gcaaaccttc
    12841 tatttttttc tatctgacac ttagcatagg aaatgattat atacgtaatc tttcaccgat
    12901 gataaattga acatatagaa acagaaacat ttaatttatt ttgaggagga gcctatctat
    12961 atgaaaaaga aagtctctat aggagaaaga agttttacac ttttcaacaa tacgtttcta
    13021 attttgctag ctttgatctg tatcgttcct tttttaaata tcatcgctac atcttttgct
    13081 tctacacaag aagtcgtcgc gaaaaagttt atcctttttc ctactacctt ttctttagac
    13141 gcgtatcgct acattctttc tacgccgacc atttttagag cacttgctgt ttcgattggt
    13201 gttacaggtg tcggaacgat cgtcagtatg tgtgcaactt cactgatggc ctacggtctt
    13261 tccagaaagt atttgtttgg tcgaggtttc gtcaactttc ttgttgtatt ttctatgttg
    13321 ttcagcgggg ggatgattcc aacgttctta gttgttcgtt ctctagggct ggtcaattct
    13381 tactgggcca tgatcctgcc tgtggctgtt aatgcaatga atatgatcat tatgagaaac
    13441 tttttccaag ccttgccgga tagtttggaa gaatcagcaa aaatggatgg ttgcacagat
    13501 tttggtgtat ttttcaaaat tatgctgcca ttagctttac cgtctattgc tactatctcg
    13561 cttttttatg ctgttactta ctggaacact tatatgactg ctattttgta tatcaacgat
    13621 tcatctaaat ggccgataca gattctttta cgccaaatcg tcattgtatc aagtgggatg
    13681 caagcagaaa gtagtgccgt tgatgtgatt ccaccagcac aaacagttaa aatggctgtt
    13741 atcgttattg caacagtgcc tatgctgatt gtttatcctt ttgttcagaa atattttgtc
    13801 aaaggagcac tcgtaggttc tgtcaaaggg tgattatgta ctgaacgcaa aatggtagtt
    13861 gtgtcagaag tggtcagctt caagaaataa gaaggcattt acgaaaattg cttcttaaat
    13921 ttttgtgaaa tttcggctta tttttcgaag aatctactcc tggaacaccg tgtatcagag
    13981 gttgtgaaag agacgttcag ccttgagcga tgataaaaac tctcacaaac attgcgacga
    14041 gtaacccaca ggagcaataa gaagcgattt tgaaaaatag cttcccatat ttcacaaaat
    14101 tatgccttat tgtcgatagc ctgtctcttg aacgccattt attcgtaaaa aagaactgtg
    14161 acaagacttt tgtcacagtt ctttttgcgt gcttagcctt gaaatttaac tatccataaa
    14221 tacaaatact agggttattc taacttcttt tattgcttga atcagtccac aagaaaaaca
    14281 gcctcttgat taaacggcgt ttatcttgtt gattctgcgg caataaaatc agccagcagc
    14341 aaaacatgaa gtgctgtttt ttgctgtcct cttttattgc ttgaatcaga tcacaagaaa
    14401 aacagcctct tgattcacaa atattttttt cgatattctc ccggcgtaat cgattccttc
    14461 tttttaaaga aacgaataaa attttgcgga tttcgatatt gcaatctttc tgcaatctct
    14521 tttacggaga ggttagtctc tttcaaccat ttttttgcta cctctaaacg atgattttga
    14581 acaaaatcac caaatttttc tcctgtttcc tttttaaata cattacttaa atagttaggg
    14641 ttataatgca acctatcagc gatcacatct aaagacagat cacgatcata ttctgtgtag
    14701 acgatatgca taatattctc cgacaaattt ttaaattcat gatcagtgat tgcttgagag
    14761 cattctgtga ttggcagaac taattgttct aaaacaagtt tttctagttc tttgggatga
    14821 taattgttca gtgcttttac ataaatttgt ttcatgttat caaagttttt tgaatctact
    14881 cccaataact gtcctaattg aacgagttca ttgatcaagc ggaccgaaac tacttcccga
    14941 ctcaaaggat ttttattttg tgcaaacaat gcatcaatca attcatgtac taaattcttc
    15001 acttcctgat ttccagaacg aatggcttca aacaatcgat tttgttcttc tatcggatat
    15061 ctaataagtg ttttttcatg agcagcagga acgatttcct gataaaaaat aattgaactt
    15121 ggaccagtat ttacacgata atacagggct tcttttgccc gatcaaccga ttgcttgctt
    15181 tcattcagcg tttcgaaccg gttactgatg cctatactga tagttaaatt caagtattct
    15241 tttacacgtt tttgaatgag tcggcaatac tccatgactt ttttgcttgc ttctgcatca
    15301 tctttatcca ttcgataaat agtggcttgc atttcttcat tcaaaacgat cggcagcatt
    15361 cgattttcct ttggcacaat ttccgtgatc atgttgttga tcgctaaaag tagcaaatca
    15421 cgctccccac cgtgactatc ttctaacaga tcgatttgaa ccaaagctgt ataatagcag
    15481 tcattttgag aactataacc aaattgctgc aaccgctgat ctagttctcg cctttctact
    15541 cgatttctaa ataaactaag tacaaatagc gtttccagct gtggcttttg ggtaactaag
    15601 tgtgcactta acgcttcttt ttctcctatg atccgactga ctgattgagc gaccaaatct
    15661 agttctttcc cactgaatcc ttttgattta agattcagac gttcctgtat ttgagaaatc
    15721 ggacgggcaa atctgtccga aaataagtag gaaaaaagac caatgagtcc taccagtgtt
    15781 aaagacactg caactagccc gattctcaga ttgcggatca ttgcgccaat tgcactagag
    15841 tcaacttcta atctgtaaat ccaattatta taatctgatt tcagtactat atacgtttta
    15901 tctttttctc ttatcacttt cagctgattg taatctagat ccacgtccgt taatacagtt
    15961 ggttgctctt tattttttac tgtctgagta gtaaaaaggt tgccaacttc gttttcaata
    16021 gaaagtatgt cttctccact ttcaccaaca acttgttcta ttgtatgttt ctctatcgta
    16081 gcaattccca atgcatattt ttctttttca aacattggca aagaaatcac catttcgatt
    16141 cctttgtttt tcttttgcca aaataaatta tttttgttat ttatgtactt ctgttgatac
    16201 ttttcaactt cctcttcggt cagttgactc aacgatccat tgattactcc ccatttacca
    16261 gccaaactga ctaaatcata attattatca tccataacaa tcgtttctat aaaatttagt
    16321 tctttcctga tttcagaata agtggagtaa tctgtatatg tcagcggatg cttgaatatg
    16381 tccgagaaat ttttggtcga accatacgta gaaaacgaat attcaatcgt tctaattttc
    16441 cattcaacgt tagttttaac ttgcctgagc aaattctttt tgtctgttaa ataactcgat
    16501 tcgatcgatt cataggtatt ctggtaaatg aataaactaa atcctatgac aagtacggct
    16561 cctaataaaa acatcggtaa aaatatttta taaaatactg tacttttctt cattttcttc
    16621 ctctcctcct tttaaaataa aaggctttca tttttctgta aaattgatac tcatctaatt
    16681 aaatgagcat tccccgcttt caagaatagc ataattaaaa ataaaaatcc aagtaatttt
    16741 taacgtgtgc aatcttttca tatcaaagat ctattgactt atcgtagtta tcgtaatatg
    16801 atgaatgcgt taacaaaaag tgaaaaagta aggaaaatga tggaatggta acgatcaaag
    16861 atatcgctaa agcagctggc gtttctcata cgacagtttc tcgtgctttg aataacagca
    16921 gtttaataaa aaatgaaaca aaacaaaaaa tacgacaact agctaaggaa atgaattatt
    16981 cgccaaacta tagtgcaaaa ggtttagtga atcaaaaaaa atatctaatc ggactatttt
    17041 tctcaagttt gcataaagga acttcatcaa atttcctagc agattccatt gcaggcatcc
    17101 attcagtatt agacacaaat tacagtttat ctgttgaaag tatcgatgtt gttgatttaa
    17161 cagaaatcaa cctgcaacgc tacgatggac tgatcgttat gagtcaaagt gatgatgatc
    17221 agcctttcat cgactatctc aaagaaaaag aatttccttt cgttgttgtc aatcgtcaca
    17281 tcaatgatcc agcggttatc aacgtcactg ccgatgatgc ggccggtgtt atgcaggcag
    17341 tagattacgg tattggatgc ggacatacga agatcgctta tattggcgga aagaaaaatt
    17401 ttcgttcgac aaaagaacgg gaaaaagggg tattgaaaag tctaaaaaaa gcgagcatcc
    17461 ctgtcaatct agcctatttt ttaacaggag attacagctt agaaagcggc ctccaaaaca
    17521 tggaatacct attatcactt tctgacctgc ctactctggt tttttgcggc aacgacgaca
    17581 tagcgattgg tgcattgaga gctgtacatt tagctggcct ccgtgttccc cagaacattt
    17641 ctctgatcgg gttcgatgat tctccagtcg tctcctatct caatccgccg cttaccaccg
    17701 tacgtaagcc tttgaaagaa atgtgtcaag aaggaacaaa gcttttacta gatttgatcg
    17761 aaggcaggac gatcgaacaa tctaaaattg agctctccac cttgttgatc cttcgtgagt
    17821 cgatcaatta cttgtaaact acaagtaatt ttatataaaa atgttaacgt gtgcataaca
    17881 cagaaaggaa gtttatatga aaaaactgac aaaagaatta gtgaaaaaaa acgaagcacc
    17941 tgtaaaagtt cttcagtttg gtgaaggcaa ttttatgcgt ggatttattg attggcagat
    18001 tcagcagctg aataaccaac aattgtttaa aggtaatgtt gcaatcattc aacctttaga
    18061 acaaggtctt ggaaatatga tgaaccagca agataattta tatacggtca ttttacaagg
    18121 actcagcaat ggcgaaatcg ttgatacttc cgagattatc accgtagtag aacaaatgat
    18181 caatccttat gatgaatggg atgcttatct atctctagca gaaaatgacg acttggccta
    18241 tatcgtctcc aatacaacag aggcaggaat ccaattcgtg gagactgata atttaacaga
    18301 tactccccca cagagcttcc cgggcaaact gacagccttt ttataccgac gtttcaaatt
    18361 aaacaaagct ggctttacca tcattccttg cgaactgatt gacagaaacg gcgaacgtct
    18421 aaaagaaatc attttacgct acgcagaaaa atgggattta gaacctgctt ttgttgagtg
    18481 gttagaaaaa gaaaatgttt tctgttgtag tttagttgat cgcatcgtcc caggttatcc
    18541 tcgcgaagat gcaggtgttt tcaatcaacg gcataactat gaagatcaat tgatggtcaa
    18601 agcagaacca ttcatgcttt gggtgatcga agggcctaaa gaattggaga atcaactacc
    18661 attcaaacag gctggcttga atgtcatcat cacagatgat atgactcctt atcgtgaacg
    18721 taaggtgcat ttgcttaatg gaccgcatac agcgatggtc cctcttgctt tgttggcgga
    18781 attggaaact gtagaagaag taatgaaaga tcctttattc tccgcatatg ttgatcagtt
    18841 attcaatcta gaattgattc ctatgctttc tttacctaaa gatgaattag ccatttatgc
    18901 tgaccaaatc aaagaacgtt tcttgaatcc ttttgcccat cacaaattag aagcgatttc
    18961 attaaatagt gtttcaaagt tcagcacccg tctgcttcca gtgttcaaga agtatatcga
    19021 agaacaaaat caggtaccac cgttaatcac tgtttcacta gctgcgctac ttcttatgta
    19081 tcgaggcgat caagtaaagc cacatgatga tgaaaagacg ataagcgaat ttactgatgc
    19141 ttggtctgat aatggaacag ctattcctcg attattgcaa aacgctgcgc tttggggaga
    19201 agatttgtct caaataccga atgtgacaga tacagttcaa gaaatagctg caaagatcga
    19261 tcaaaaaggt gtccggtcag tattacaaga aatgaaagga acgatggtcc atgcttgaaa
    19321 ccctgcaacc aacaatgaaa ttaaatcgac gagacagtgt ggtcgtcgca ttaaccccta
    19381 ttccaaaaca aacacaactg acgattgatc agcaaacgat caccactctt gaagatatcc
    19441 cgcaaggtca taagattgca ttgggtgatt tgaaagctgg agacaacgtg atcaaatatg
    19501 gctatccaat cggtcatgtg acgacagacg taactgccgg tcaatggctg cacactcata
    19561 atgtaaaaac caatctttct ggtgaattgg attatcgata tgaaaaagat gtccatccaa
    19621 gtcattacct ttttgagaac cgaacatttc aaggttactt gcgaaaaaat ggaaaagtag
    19681 ggattcgtaa tgatttgttt ttagtaccta ccgttgggtg tgtgaatggc attgctgaac
    19741 tgatcgtcaa acagttcaag gaaaaacatc cagatctggg acagtttgac catattacga
    19801 ttttaaagca tccttatggc tgttcgcagc ttggaaaaga tcatcaaaat actcgagaaa
    19861 ttttagcgga cgcagtaaat catcccaatg ccggaggagt tttagtattt ggattaggtt
    19921 gtgagaacaa tacagttcct gaattcaaaa aaattttagg tgaatatgat gaagaacgag
    19981 tgaaatttct agtggctcaa gatgtttacg atgagatcga acaaggtgtg gctctattag
    20041 aagagctttt aacagcggcg gaaaatgacc agcgcatatc agtcccgctc tctaagttga
    20101 atgtcggttt gaaatgcggc ggttctgatg gtctatcggg aataactgct aatcctttac
    20161 ttggtgcatt ttctgactac ttgattgctc aaggaggcag cacgatctta acagaagtgc
    20221 cagaaatgtt tggcgcagaa caagtattga tggcacgagc ggaaaatgaa gaagtttttg
    20281 actcaatcgt tgacttgatc aatgacttca aacagtattt cttgtcttac ggggaaccag
    20341 tttacgaaaa cccatctcct ggtaataaag caggtgggat cactacatta gaagataagt
    20401 ctttaggctg cacgcaaaaa tcaggaagct ctcctgtcgt tgatgtgcta caatatggcg
    20461 aaaaaatcag aaaaccaggg cttagtttat tgcaagctcc cgggaatgat cttgtagctg
    20521 cctctgctct tgcttcatcc gattgccagt tggtcttgtt taccacagga agagggactc
    20581 cgtttggctc atatgtccca acggtcaagg tatctacgaa tacaactctt tttgaacgca
    20641 aaggtcattg gatggatttt aatgcaggcg tcttattaga acaaccaatg gaagtaatct
    20701 tagaatcgtt tgttcaaaaa atcatcgctg tagctagtgg agaagaaacg aaaaatgaac
    20761 aaaatgacgt tcgagaaatt gccattttta aaaacggagt aacactttga ttttcataaa
    20821 acagtataaa aagaaagaag gaagatttat gtttttatct gatgattttt tattaaaaga
    20881 cgattgggcg aaaaagcttt accattccta cgcaaaaaac atgcccatta ttgattatca
    20941 ctgtcactta gatccaaaag aaatttacga aaacaacaat ttcagcaatc tgacagaagc
    21001 ttggttagga ggcgatcatt acaaatggcg cttgatgcga gcttgcggcg tgcctgaaga
    21061 aaaaatcaca ggaaatgctt ctgattttga aaaattttta gcttggtgtc aaaccgtacc
    21121 aaaaattatc ggaaatccgc tttacagttg gtcacatctt gaattgaagc gtttcttcgg
    21181 tattgacttg ttgataaatg aagaaaatgc agagaaaatt tggacacaag caaatgaaat
    21241 tttggcagct cctgattttc gccgtcggga aatcgcaaaa aaatcgaatg tagaagtgat
    21301 ttgtacaaca gatgatcccg cagacgactt gcactatcat cagttgcttg ctcatgaaga
    21361 accagatttg aaggttctac cttcattccg tccagataaa gcactcaata ttgaaaaaga
    21421 aggatttaat gcatgggtca cactactgga agaagcagct gaccaaacga tcaccaccta
    21481 tcaagaactg atagaagtct tagggcagcg gatagaatac ttccatcaac atggatgtcg
    21541 tgtttccgac cacgctttag acatcttacg ctataaagaa gcggatgaaa cagtattaga
    21601 agagattttt caaaaagcac gagctaacaa actattatct tcagatgaaa tcgatgctta
    21661 tcttacagaa acactgatac atttgaccca cttctatcat tctcataatt ggacgatgca
    21721 gctgcacatc catgcttatc gtaactgtaa tacacaaatg ttggaaaaat tagggcccga
    21781 taccggctat gatggaatga atgatctttc tttgactatt cctctgcaaa aattattgaa
    21841 ccgtgcagaa gaaacggatc agttgccaaa gacgatcctt tattcattga atccgaatga
    21901 ctatcctgcg attttagctt tgatgggctg tttccaaaaa gaacagaatg gaaaactgca
    21961 gttaggttct ggatggtggt acaacgatac tcgagctggt atgcgcgatc aattgacaca
    22021 atttgctgat ggtagtgctt tggggaattt tgtcggcatg ctgacagatt caagaagttt
    22081 cctgtcctac actagacatg aatatttccg tcgtgttctt tgtgaatttt taggcgaaat
    22141 ggtcatgcga ggagaagcac ctgaagatga acaactttta ggaagtctag ttcaagcgat
    22201 tagttataca aatgctaaaa attactttgg cttttttgcc taattgatta atagtaacag
    22261 taacgaatca aaaacaggat agtgaaataa cggaatccgt ttatccacgt tttagagaag
    22321 aagacggacc aa
//
LOCUS       DASJPC010000038        22031 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJPC010000038 DASJPC010000000
VERSION     DASJPC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.038"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            367..555
                     /locus_tag="U4A13_001451"
     CDS             367..555
                     /locus_tag="U4A13_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779751.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="U4A13_001452"
     CDS             545..805
                     /locus_tag="U4A13_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779752.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="U4A13_001453"
     CDS             846..1073
                     /locus_tag="U4A13_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779753.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="U4A13_001454"
     CDS             1138..1710
                     /locus_tag="U4A13_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779754.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="U4A13_001455"
     CDS             1711..2022
                     /locus_tag="U4A13_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1779755.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="U4A13_001456"
     CDS             2013..2273
                     /locus_tag="U4A13_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779756.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="U4A13_001457"
     CDS             2434..2793
                     /locus_tag="U4A13_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779757.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="U4A13_001458"
     CDS             2790..4433
                     /locus_tag="U4A13_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1779758.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4447..5550
                     /locus_tag="U4A13_001459"
     CDS             4447..5550
                     /locus_tag="U4A13_001459"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779759.1"
                     /translation="MKGVVIEVKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFT
                     TKVDDSVTSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAEN
                     ERAKKELLTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSK
                     DLSDSEIGSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILN
                     EQYEKMQIYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVI
                     LFNSLYIKRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKR
                     QKLSEYIKELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="U4A13_001460"
     CDS             5594..6766
                     /locus_tag="U4A13_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1779760.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="U4A13_001461"
     CDS             6753..7457
                     /locus_tag="U4A13_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1779761.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="U4A13_001462"
     CDS             7462..8622
                     /locus_tag="U4A13_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1779762.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="U4A13_001463"
     CDS             8698..8976
                     /locus_tag="U4A13_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN1779763.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="U4A13_001464"
     CDS             8957..9328
                     /locus_tag="U4A13_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEN1779764.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="U4A13_001465"
     CDS             9344..9748
                     /locus_tag="U4A13_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779765.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="U4A13_001466"
     CDS             9745..10155
                     /locus_tag="U4A13_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779766.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="U4A13_001467"
     CDS             10216..10803
                     /locus_tag="U4A13_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1779767.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="U4A13_001468"
     CDS             10876..11343
                     /locus_tag="U4A13_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEN1779768.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="U4A13_001469"
     CDS             11389..11697
                     /locus_tag="U4A13_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779769.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="U4A13_001470"
     CDS             11892..18626
                     /locus_tag="U4A13_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1779770.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="U4A13_001471"
     CDS             18630..19502
                     /locus_tag="U4A13_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEN1779771.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="U4A13_001472"
     CDS             19499..20548
                     /locus_tag="U4A13_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779772.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="U4A13_001473"
     CDS             20552..21376
                     /locus_tag="U4A13_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779773.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="U4A13_001474"
     CDS             21394..>22031
                     /locus_tag="U4A13_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEN1779774.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DASJPC010000039        21988 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJPC010000039 DASJPC010000000
VERSION     DASJPC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.039"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..395
                     /locus_tag="U4A13_001475"
     CDS             <1..395
                     /locus_tag="U4A13_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779775.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="U4A13_001476"
     CDS             398..1147
                     /locus_tag="U4A13_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1779776.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="U4A13_001477"
     CDS             1159..1428
                     /locus_tag="U4A13_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779777.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="U4A13_001478"
     CDS             1433..1597
                     /locus_tag="U4A13_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779778.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="U4A13_001479"
     CDS             1590..1892
                     /locus_tag="U4A13_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779779.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="U4A13_001480"
     CDS             1889..2050
                     /locus_tag="U4A13_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1779780.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="U4A13_001481"
     CDS             2047..2352
                     /locus_tag="U4A13_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779781.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="U4A13_001482"
     CDS             2352..2708
                     /locus_tag="U4A13_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEN1779782.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="U4A13_001483"
     CDS             2695..2913
                     /locus_tag="U4A13_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779783.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="U4A13_001484"
     CDS             2910..3329
                     /locus_tag="U4A13_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEN1779784.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="U4A13_001485"
     CDS             3326..3883
                     /locus_tag="U4A13_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1779785.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="U4A13_001486"
     CDS             3880..4176
                     /locus_tag="U4A13_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779786.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="U4A13_001487"
     CDS             4253..4666
                     /locus_tag="U4A13_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1779787.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="U4A13_001488"
     CDS             5012..5386
                     /locus_tag="U4A13_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779788.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="U4A13_001489"
     CDS             5423..5590
                     /locus_tag="U4A13_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779789.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="U4A13_001490"
     CDS             5616..5960
                     /locus_tag="U4A13_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN1779790.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="U4A13_001491"
     CDS             5965..6246
                     /locus_tag="U4A13_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779791.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="U4A13_001492"
     CDS             6349..6663
                     /locus_tag="U4A13_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN1779792.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="U4A13_001493"
     CDS             6641..8335
                     /locus_tag="U4A13_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN1779793.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="U4A13_001494"
     CDS             8355..9533
                     /locus_tag="U4A13_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN1779794.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="U4A13_001495"
     CDS             9496..10182
                     /locus_tag="U4A13_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN1779795.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="U4A13_001496"
     CDS             10182..11342
                     /locus_tag="U4A13_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN1779796.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="U4A13_001497"
     CDS             11352..12227
                     /locus_tag="U4A13_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779797.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="U4A13_001498"
     CDS             12224..12535
                     /locus_tag="U4A13_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779798.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="U4A13_001499"
     CDS             12525..12878
                     /locus_tag="U4A13_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN1779799.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="U4A13_001500"
     CDS             12868..13269
                     /locus_tag="U4A13_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779800.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="U4A13_001501"
     CDS             13262..13666
                     /locus_tag="U4A13_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779801.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="U4A13_001502"
     CDS             13678..14286
                     /locus_tag="U4A13_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1779802.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="U4A13_001503"
     CDS             14306..14668
                     /locus_tag="U4A13_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779803.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="U4A13_001504"
     CDS             14671..14853
                     /locus_tag="U4A13_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779804.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="U4A13_001505"
     CDS             14870..18289
                     /locus_tag="U4A13_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN1779805.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="U4A13_001506"
     CDS             18340..19077
                     /locus_tag="U4A13_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1779806.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="U4A13_001507"
     CDS             19087..21378
                     /locus_tag="U4A13_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN1779807.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="U4A13_001508"
     CDS             21402..>21988
                     /locus_tag="U4A13_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779808.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DASJPC010000040        21911 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJPC010000040 DASJPC010000000
VERSION     DASJPC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.040"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4A13_001509"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4A13_001509"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN1779809.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="U4A13_001510"
     CDS             complement(1172..1867)
                     /locus_tag="U4A13_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN1779810.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="U4A13_001511"
     CDS             complement(1880..2596)
                     /locus_tag="U4A13_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN1779811.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="U4A13_001512"
     CDS             2926..3534
                     /locus_tag="U4A13_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN1779812.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="U4A13_001513"
     CDS             3859..4182
                     /locus_tag="U4A13_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN1779813.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="U4A13_001514"
     CDS             4364..5734
                     /locus_tag="U4A13_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN1779814.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="U4A13_001515"
     CDS             complement(5864..7165)
                     /locus_tag="U4A13_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1779815.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="U4A13_001516"
     CDS             complement(7519..9477)
                     /locus_tag="U4A13_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN1779816.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="U4A13_001517"
     CDS             complement(9654..9920)
                     /locus_tag="U4A13_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1779817.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4A13_001518"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4A13_001518"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN1779818.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4A13_001519"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4A13_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN1779819.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="U4A13_001520"
     CDS             13264..13827
                     /locus_tag="U4A13_001520"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN1779820.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="U4A13_001521"
     CDS             complement(14080..14601)
                     /locus_tag="U4A13_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN1779821.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="U4A13_001522"
     CDS             complement(14604..15446)
                     /locus_tag="U4A13_001522"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN1779822.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="U4A13_001523"
     CDS             complement(15439..16164)
                     /locus_tag="U4A13_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN1779823.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="U4A13_001524"
     CDS             16340..16636
                     /locus_tag="U4A13_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779824.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="U4A13_001525"
     CDS             17204..18796
                     /locus_tag="U4A13_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1779825.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="U4A13_001526"
     CDS             18809..19549
                     /locus_tag="U4A13_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1779826.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="U4A13_001527"
     CDS             19717..21066
                     /locus_tag="U4A13_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1779827.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="U4A13_001528"
     CDS             complement(21194..21784)
                     /locus_tag="U4A13_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1779828.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6294 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa a
//
LOCUS       DASJPC010000041        21732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJPC010000041 DASJPC010000000
VERSION     DASJPC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.041"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(152..550)
                     /locus_tag="U4A13_001529"
     CDS             complement(152..550)
                     /locus_tag="U4A13_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN1779829.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="U4A13_001530"
     CDS             complement(547..1320)
                     /locus_tag="U4A13_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1779830.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="U4A13_001531"
     CDS             complement(1317..2000)
                     /locus_tag="U4A13_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN1779831.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="U4A13_001532"
     CDS             complement(2167..3054)
                     /locus_tag="U4A13_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1779832.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="U4A13_001533"
     CDS             complement(3051..3920)
                     /locus_tag="U4A13_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1779833.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="U4A13_001534"
     CDS             complement(4057..5343)
                     /locus_tag="U4A13_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1779834.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="U4A13_001535"
     CDS             5616..6194
                     /locus_tag="U4A13_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN1779835.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="U4A13_001536"
     CDS             6194..7123
                     /locus_tag="U4A13_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779836.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="U4A13_001537"
     CDS             7120..8856
                     /locus_tag="U4A13_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1779837.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="U4A13_001538"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="U4A13_001538"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN1779838.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="U4A13_001539"
     CDS             complement(9752..10261)
                     /locus_tag="U4A13_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN1779839.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="U4A13_001540"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="U4A13_001540"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN1779840.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="U4A13_001541"
     CDS             complement(11562..12653)
                     /locus_tag="U4A13_001541"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN1779841.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="U4A13_001542"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="U4A13_001542"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN1779842.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="U4A13_001543"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="U4A13_001543"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN1779843.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="U4A13_001544"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="U4A13_001544"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN1779844.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="U4A13_001545"
     CDS             complement(16616..17590)
                     /locus_tag="U4A13_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN1779845.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="U4A13_001546"
     CDS             complement(17571..19454)
                     /locus_tag="U4A13_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN1779846.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="U4A13_001547"
     CDS             complement(19472..21697)
                     /locus_tag="U4A13_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN1779847.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         6198 a         4790 c         3859 g         6885 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac tt
//
LOCUS       DASJPC010000042        21372 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJPC010000042 DASJPC010000000
VERSION     DASJPC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.042"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            53..754
                     /locus_tag="U4A13_001548"
     CDS             53..754
                     /locus_tag="U4A13_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN1779848.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1363..2379)
                     /gene="tsaD"
                     /locus_tag="U4A13_001549"
     CDS             complement(1363..2379)
                     /gene="tsaD"
                     /locus_tag="U4A13_001549"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN1779849.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2376..2840)
                     /gene="rimI"
                     /locus_tag="U4A13_001550"
     CDS             complement(2376..2840)
                     /gene="rimI"
                     /locus_tag="U4A13_001550"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1779850.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2847..3389)
                     /gene="rimI"
                     /locus_tag="U4A13_001551"
     CDS             complement(2847..3389)
                     /gene="rimI"
                     /locus_tag="U4A13_001551"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN1779851.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3373..4095)
                     /gene="tsaB"
                     /locus_tag="U4A13_001552"
     CDS             complement(3373..4095)
                     /gene="tsaB"
                     /locus_tag="U4A13_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN1779852.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4286..5266)
                     /locus_tag="U4A13_001553"
     CDS             complement(4286..5266)
                     /locus_tag="U4A13_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1779853.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5290..6774)
                     /gene="galT"
                     /locus_tag="U4A13_001554"
     CDS             complement(5290..6774)
                     /gene="galT"
                     /locus_tag="U4A13_001554"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN1779854.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6786..7775)
                     /gene="galE"
                     /locus_tag="U4A13_001555"
     CDS             complement(6786..7775)
                     /gene="galE"
                     /locus_tag="U4A13_001555"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN1779855.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8023..8190
                     /locus_tag="U4A13_001556"
     CDS             8023..8190
                     /locus_tag="U4A13_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN1779856.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8252..10063)
                     /locus_tag="U4A13_001557"
     CDS             complement(8252..10063)
                     /locus_tag="U4A13_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN1779857.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10060..10425)
                     /locus_tag="U4A13_001558"
     CDS             complement(10060..10425)
                     /locus_tag="U4A13_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN1779858.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10588..10983)
                     /locus_tag="U4A13_001559"
     CDS             complement(10588..10983)
                     /locus_tag="U4A13_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN1779859.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11001..11963)
                     /locus_tag="U4A13_001560"
     CDS             complement(11001..11963)
                     /locus_tag="U4A13_001560"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN1779860.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11963..12175)
                     /locus_tag="U4A13_001561"
     CDS             complement(11963..12175)
                     /locus_tag="U4A13_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN1779861.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12196..12894)
                     /locus_tag="U4A13_001562"
     CDS             complement(12196..12894)
                     /locus_tag="U4A13_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN1779862.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12914..13456)
                     /locus_tag="U4A13_001563"
     CDS             complement(12914..13456)
                     /locus_tag="U4A13_001563"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN1779863.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13588..14592)
                     /locus_tag="U4A13_001564"
     CDS             complement(13588..14592)
                     /locus_tag="U4A13_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1779864.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14589..15578)
                     /locus_tag="U4A13_001565"
     CDS             complement(14589..15578)
                     /locus_tag="U4A13_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN1779865.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15575..16381)
                     /locus_tag="U4A13_001566"
     CDS             complement(15575..16381)
                     /locus_tag="U4A13_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779866.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16547..17503
                     /locus_tag="U4A13_001567"
     CDS             16547..17503
                     /locus_tag="U4A13_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1779867.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17580..18098)
                     /locus_tag="U4A13_001568"
     CDS             complement(17580..18098)
                     /locus_tag="U4A13_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1779868.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18186..18335)
                     /gene="rpmG"
                     /locus_tag="U4A13_001569"
     CDS             complement(18186..18335)
                     /gene="rpmG"
                     /locus_tag="U4A13_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1779869.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18563..19009
                     /locus_tag="U4A13_001570"
     CDS             18563..19009
                     /locus_tag="U4A13_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN1779870.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19204..21099)
                     /gene="asnB"
                     /locus_tag="U4A13_001571"
     CDS             complement(19204..21099)
                     /gene="asnB"
                     /locus_tag="U4A13_001571"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN1779871.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6424 a         4545 c         3692 g         6711 t
ORIGIN      
        1 ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt
       61 taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc
      121 ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca
      181 gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt
      241 tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa
      301 taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc
      361 gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa
      421 aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga
      481 attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt
      541 cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc
      601 gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt
      661 aatcaaacaa ctacgtgatt ccgggctgtt agattatgac aaaaagcgtt ttactttctg
      721 taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct
      781 gataaatatc agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt
      841 ttagatcact ggacaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac
      901 tgtttgcaaa aaatactgtt agattaacta gatgaagcgt cttgtcctca tccgatctag
      961 attattcgat attcctcgac aataaacgat tactcagtga gggcaatcag aggaacggat
     1021 aatgataaat aagctgtaaa aaatagcaaa ataacaataa tgattaaaag tatgtaaaaa
     1081 atagggcttt gacaaaaacg tgtcacagcc ctatttcgat taaacggtgt gtacctgtca
     1141 actcttcggt cacaagtcat aatgttcata aacatgagga gctgtttttt gaaattgttc
     1201 cttgtgaatc agagttaaac gacaggctta cgaactcttg attaaacggc gttcaaaagc
     1261 ttgcatacgc ggtaataaga acagccaggt caaaaacatg agaagccgtt tttcctgggc
     1321 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctttattcag ataaaaccaa
     1381 gcttggatca gcattcaact gatagctagc aaaatttctt ttttgcattt ccacatgtgc
     1441 tgctgctcca atcattgcag cattatctcc acaaagtcga agagggggaa tcactagttc
     1501 tacctcaggg agtttagctg ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc
     1561 cgctacacct ccagctataa tcagttgctt gactggataa ttttgacaag ctcgaagtgt
     1621 tttagtaatc aatacatcga tcacacttgc ttgaaagcta gctgctaaat catttttgtc
     1681 tagatcttct ccacgctgtt gtgcattatg caccaaattg ataaatgcac tttttaatcc
     1741 gctaaaactg aaatcataat tatcttcatg gatcatcgct cgcggaaaat ggtaattatc
     1801 ttttccctga tgagctagct gatcgatttc ttttccgctt gggtaagaaa gacctaatac
     1861 tcttccaact ttgtcataag cttctcctgc tgcgtcatct cttgtctccc caatgatttc
     1921 atatgatcca tcttcttgca tatagactaa ttctgtatgt cctccgctaa caagaagtgc
     1981 catcaatgga aactggaatg gtttaaccag acgggctgca tagatatgcc ctgccatatg
     2041 gtttacagga ataagcggca attggtgtgc ccaagcaaaa gccttagctg cagaaatacc
     2101 aatcagcaac gagccgacaa gtcctggtcc ataagtgaca gcgactgccg ataaatcttc
     2161 tgccgacaca ccagcttcta ccagtgcatc ttccaagcat aaggtgattt gttctacgtg
     2221 atggcggcta gctacttcag gtactacgcc accaaaacgt ttatgactgt ttatttgaga
     2281 agcaacgatg ttagataaga tctctgttcc atttctaacg accgctacac tcgtttcatc
     2341 acaacttgat tcaatcgcca gaatcaattc ttcattcatt tcatcgtctc cgtttttaac
     2401 tttctgctca taatgatcgc atcttccgtc ggattgcgat aatatgattt ccgtttacct
     2461 aatatacgaa acttttctga ttcataaagt tttcttgctg cttcattaga tagacgtact
     2521 tctaaaaaaa catgcagcga atcttttatt tgttccgtaa tcagtaattg taacaaatgc
     2581 cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa tatttgtgat ctcgacttca
     2641 tctattactt tcgagtatcc aataaagcca cagatctggt tatcttcagc taaaagcaga
     2701 taatcagagt gaggctgttg gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca
     2761 tgtggataag cattttcgct aatcatccat acgcattctg ccaagtacgc ttcatcaaaa
     2821 tctttttttc tgcaaatcaa ctaatctcac tcattttcta aaagttttat catatccaac
     2881 gcatcttctt ttgtttcagt gtaatatctt tttttgatcg ttgttgcttg aaaccccaat
     2941 ttccgataaa gtctttgtgc atcttgatta ctcatacgca cttctaatga catcgtctca
     3001 cagcgattca ttatagcaaa gttctcgatt tcgtcaataa gcagcgaacc gattcgttta
     3061 cgctgatatg ccggtcgaac ggcaatattc gtgatatggc aatctaaacc gaacgctctt
     3121 gaaccgataa aaccaatcat tcttccatta tcaaagatac ctagatatag atgaggaagc
     3181 ggtgacttca attctgatat aaaggcactt tttgtccatg gcaattcgcc attatagact
     3241 tctcgttcga catccagcag ctctttgata tcttctgtag tcaattctct tagtacgtaa
     3301 cgacgatcga tttgttcgac tgttttttct gtataaatca ttttatcttg aaaaaagctt
     3361 ctggcatacg ctctaaattt tttcaacata actttcgtct ccaggtgtgt gcgtctccaa
     3421 ccatttttct tctgcttcta ctcgttttaa atagcgagga agaaaagctt gtttctctgc
     3481 aactggttca actaaagcac ctaattgagc cacagttaca ccacttggaa tatcccatgc
     3541 tggaactgta ttaatcatcg cactaggcaa tatttccttt atttcagaga agaatttgtg
     3601 acaatcactc ccgacgaaat aaatagcttc attcagcttt tgtagttgtg tgaacaaatc
     3661 agaagcagaa atatgtacat cttcaagtac agtttctaat ctattttctt tccagcggta
     3721 tgccccagcg tatacatttt ttcttcgagc atcaaacatg ggcacaataa tattatccac
     3781 accgatgcaa ttagccgcga tggttttcag actagatact cctactagct ctttatctaa
     3841 tgtataagca atcgtttttg ctgtcgttac acctatacga agccctgtat aagaaccggg
     3901 accatcagaa accgcgattc ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa
     3961 ttggtcgatt gcaggcatca gtgttacact gtggttgcgt ttgatatttg tttgtatttg
     4021 tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa gtttggtttg cagtatctat
     4081 tcctaatgta atcatatatt tgatcctttc tgctatgaca ttctcgcctt ccattgtagc
     4141 atactttttt attctgagga tgaaaatacc aaaggcaaaa ctagtacaat aatacatgat
     4201 tatctgctgg cgggcaaatt ttgccccttc ctgaaacaca aaaaagctgc ggtatcactc
     4261 cattttccca cagcttttcc atcttttatt tcgttgattc tcgaataatc aactctgttt
     4321 ccacaataat ctttcttgaa accggtgctt tggaattact taattgaaga atcgtctcca
     4381 ctgctagctc tcccatccat tcggtataaa cttttacagt agaaagcgag ggcgttacat
     4441 atttcgctac actgacatca ttgaagccca tcacagaaat atcttctggt accactaaac
     4501 ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc atcattagct gcaaaaagcg
     4561 catcaggaac atgcggattc gtttttaaat attgcttcac tgcttgatat cctgactcga
     4621 cagaaaaatc tgcttcgata attggacctt cgtcgtagcc tttttctgcc aaaacttgtt
     4681 gaaatgccat aattctggga tcttttagcc gctgatggtt tttcttcgta tattctgttc
     4741 cagcaagcat agcgatatgt tgcgctttat gggagacaaa gtgttcaatg actttttgaa
     4801 cacttgactt aaagtcgaca actagagagt cgattccttg agaagtgccg tcagaatcta
     4861 ccaaaagcaa ggcattttgg gatcctttca tttctaataa ctgagcttcg tcgaattttc
     4921 ctagtgcgat cgttccatct gccggaagat cactaatgga atcccacgtt tctttcaata
     4981 aagtaacgtt tgcttcttct gccttttttt cgattcctag gcgaatagat aaataataaa
     5041 gatcttcaag ctcttccgta tcgtcgtacc actggatcag gcgaagcgtt tgcttagtat
     5101 ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc tgctgcttca aaaatttttt
     5161 gctttgtggc taagccaacg gaaagatcag gatcatagtt gagaactctt gaaacagtag
     5221 caggagaaac tcctgccaac tgcgcaatgt ctttaattgt tgccatttgt ctctcctcct
     5281 tttttacgtt tataaaatcg cagtaaatcg ttcaaacgct gcctgtcctt tttcatctcg
     5341 cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc tgacctacag atttttgtac
     5401 gacatcggtg accgtatctt tcgtcaaatc aggatgttct ttttttaatt gttctgccca
     5461 cggacgatga tattcagcca tctggttttc ttctcctaat aagaaatgct ctacttctac
     5521 tagttctggt ttcaaacgag gtggtaaaac agcaagcccc attacttcga tcaacccaat
     5581 gttctccttt ttgatatgtt ggacatcttt atgtggatga aaaatcccat caggatgttc
     5641 ttctgaaaca ttgttgtctc gaaggacaag gtccaactca aagtaattgt cccttcttcg
     5701 tgcaattggc gtaatcgtat ggtgtggtgt accatctgct gaaaaagctc tgatagatac
     5761 tgtttcatcc gaatatgctt tccatttttc caatactgag ttagctgctt caataagcaa
     5821 gtttttctct gttgattgca aacggatcac agacatcggc catttcacga ctcctgcgat
     5881 gacttgcgga taatcttcta gtttgataag tttttctatc ggtgcttttg ccataggaaa
     5941 ttcatgacgt cctgcctgat aatgatcatg agaaagaatc gatcctccaa caattggtaa
     6001 gtcagcattc gaaccaacaa agtaatgagg caagacttca gtaatccgaa gcagtctagc
     6061 aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct gataaaatga tcgaatgttc
     6121 attgtaataa gcataagggg aatattggaa tccccagctt tcaccgtcta gattcattcg
     6181 aataatccgg tgattggtgc gggctggata gttcaagcga cctctatatc cttcgttttc
     6241 catgcaaagc atgcaggtag gatagtctac ttttaccgcc tcgcgttctg ctgcgatttg
     6301 ttttggatct ttttctggct tagataaatt gattgtgatt tccagttcac catactccga
     6361 ctctgccgga aatttgatgt tttttgcgat ggctcttgtt ttgatgtagt catttttctt
     6421 acttaaatta aagaaatact cagttgcttc ctctgggctt ttcgaatagt gctgtgcaaa
     6481 gaatgcattg acgactgaag gtggcggtgt gagaaaatcc attaattgcg cttcaaaaat
     6541 ctcttgttct gctggcaatt ctgaaagaac accattttct cttgccttag cgacaagctg
     6601 atccaataag tctacagatt cttccttaac tggtcttact tcaatgcttt ctagagcatc
     6661 ttctcctacc atccctaaaa gacgattctg taaatataag cggtccattt ccatccatcc
     6721 gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt tgactgattg acatatctca
     6781 ttcccctatt cttcataacc atttggatgg ctgacatgcc aatcccatgc tgttttgata
     6841 atcgctttga tatctgtgta ttcaggtttc cagcccagaa tctctctcgc tttttcactt
     6901 gaagcgacta atcggctagg atctccagct cttcttggtg ctatttctgc tggaatttct
     6961 tttcctgtca cttctctagc tgcttctagc atttccttga ctgagtatcc tttgctgctg
     7021 cctaaattga agaagttgct ttcatttcct tctttcaagt attccaaagc taaaatatgt
     7081 gcagcaatca aatcttctac ttgaacataa tctctgatac atgttccatc tggtgtatca
     7141 taatcgtctc cataaacagc caaggctttt ctttgaccaa gagctacttg taaaatgatt
     7201 gggacaagat gcgtttctgg tgtatgatct tctccgatag atgcatctgc ttttgctccg
     7261 gctacattga agtagcgcaa agcgacatag cgcatgccat atgcttggtc acaccacttc
     7321 atcatttttt ccatcatcaa tttactttca ccatacggat ttttaggatt agctggtgta
     7381 tcttctgtga ttggagactc tttcggctct ccataagttg ctgcagtaga agagaatacg
     7441 attttattca cgttgaactc gtgcatgact tcaagtaaga tttgcatacc gtatacgttg
     7501 ttgttgaaat acatcaatgg tttttcgaca gattctccta ctaaagagct tgcagcaaaa
     7561 tggattactc cttcaatcgg ttccttttca aatacggaac gtagaaattc tttatcccgg
     7621 atgtctcctt cataaaaatg agcatctgga tgtactgctt gtttgtgacc tgtgagcaaa
     7681 ttatctacca cagctacttg ataccccttt tgaactagct ggtctacagc gtgcgaaccg
     7741 atataaccgg ctccgcctag tactaaaatt gtcataaaaa acactccttt gattagtaaa
     7801 attgtgtttg ttgattctat ataatttaaa aaacgaagtt tacttttatg gtgaaaacaa
     7861 acaaagtaaa atttcgtaaa ttcatatggt aaatttataa taaaacattt agtaaacatt
     7921 ttcaagggtt ttttgcaaag gtttacaaac aaacgtaaaa ctagcctttt tttctctttt
     7981 gttccataat agatataaca aataataaat gaggtggatt caatgaaaaa atttgtatct
     8041 ggaatattag taggaagtct ggcaacagcc gctgccgttg ctggtttggt tgcttctgtg
     8101 aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa tgatcgaaga aaaccgtaaa
     8161 aaagcaatgc gtaaacgcgt ttctcgttaa taattattta aaaatacgca tgaagacttt
     8221 ctaaaaaaca gataagtcat catgcgtatt tttatttgtc agtcaatata agttgtgcta
     8281 ttttatctgc ctgtctcaga ttccatggat ctgtataagg tctagctact gctgcagtta
     8341 gccaatccac accttcaaca ggtattccat agcaataaaa gtcagactgg atttcccctg
     8401 aagcatcaat cacgcgattc gtacctcgct caacatctat agcttctgaa tgataacttc
     8461 ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt ttccttcaaa ttttttatga
     8521 caggattact tgaacgagaa aagcttgttt gtggcaatcg agcttcaatc aaatagcgac
     8581 tgctgatttc tttatcttgt agagaaataa catagcggtt gtgcttttgc tgaatatcaa
     8641 atggcggttc gatcaaatga aaaatacccg catctatcag tgctgctaat tctttcgtcc
     8701 gaatgattgg cggaccgatc gttaaaaagc tatttagatg agtaaaccat ccccatagta
     8761 tttctttgta ttccttagca gaaaaaacgt gccattcaat agcctgccgg attggatcac
     8821 gccagtcttt taatgcatca aatgttgaag caaatgctcc tgtgcaattg cctttttccg
     8881 cttcttttat ctgcccttca ataaatattc ggctagcttc tgaaaagggc tcattctgat
     8941 aggcaagagg agtttctaaa tacgaccaat cccatctata agggattagt gctggatgct
     9001 ttccgagaca ctcctctgaa gagagggaga gaaaatcatg ctcaaaattt ttctttggaa
     9061 tcggcaagtg atattcttcg atgattttct tgtaataaaa caactcaacg tcttttttca
     9121 gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg atgccattgc tccaggtttt
     9181 cttttgttag taaccgtggc catgccattg ctccgccttg cttttgattt cttcctcgag
     9241 ggtaataagg gagccccctt cttgatccac tgtatacaat cggttccttg cctgatggat
     9301 gatagaccag ttttcccttt tcgttctcga agcgccctcc tcttgatact gtaaacaatc
     9361 cgacataatc aaaaaaactt aagcctagac ctcttaaaaa gacattttct ttaggcggta
     9421 tcttcgaaag atctacatct gcaggattag aaggaagctg atacagtaaa acatttttct
     9481 ttgcgtagtc tacccacact ctttcttctt ctgtctcatc attttcccag tgaccgcttg
     9541 ctaaaacaag ctgatcgata gaatagcttt gctgatctgt ctgtaaaaga taaccatctt
     9601 ttcttttgct tacttttttt actaaagaat aaacaaactg aatagaagct ggaaattctt
     9661 cttgcaactt tgagaaaaac cagcgttgat agagtccgta taaacatcga gtagattgct
     9721 gatctttttc cagacgattg atttcttcta taattagttg cttatttttg gttaccgtct
     9781 gttcgatata atctgtagca ggtcctttac tccattgaaa caagttgggt ccctgtgtta
     9841 ccaatcctcc actggacaat gtttcatctg taaaaagcgt cacttgttga gaaacagaat
     9901 tcattaataa ctccggtggc tgattcaacc gccaaactcg acctccagga ccattgggat
     9961 cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt atggagaatc ctttctgcta
    10021 cacttaatcc tctaggtccg gcaccaatga tccctatttt catttgcatg cctccatttc
    10081 gataaatttt ttcaataaaa cagcatggtt ctgatgttca ttttttgcac cataaacaaa
    10141 agtaatgact ggatgttctt tcataagttg actgagttta aaaatagctt gcttcttttc
    10201 ttcttcgaaa atcaattctt gctggtattt ctttgcaaat gaagcaaatc gttctggtac
    10261 atggtcaaaa gttttgcgtg cttcattact aggtgcaatt tctttcaacc acagatctaa
    10321 ctgcgctttt tcttttgaaa ttcctcgtgg ccagaatcta tccacgagta ttctatagcc
    10381 atcctctttt tcagcatcaa ggtaaacacg ttttaatttg atcatagttg tttccttctt
    10441 tctacctttt cgcttagctt atatatgtag ataatagcac aaaataaaaa aaatgaacgg
    10501 cacaactatc cattgcgccg ttcatgtgat cttggtcaaa atctaatgat tgattcggtt
    10561 tttaaagctg tcatacaatc atctgtttta tactttttta cttttgattt ttccagacca
    10621 tccgtcatat ccacccttaa gtaaataaat ctcttgataa cctgctttac gtaatttatt
    10681 tgctgtacgg atgctcaaac ttttcttttg atcgtaaata tagacaggct ggtcttttcg
    10741 aatagatcct aatgaatctt tcaaaacagt gtaagggatg tttcttgcac ctaaaatatg
    10801 cccagcatca aacacatctt tttcgcgaac atcgattact tgtgcttttc tcattccttc
    10861 tcgaaattct tcttctgtca aagtcgttgc tgaacgtttg gccattactc gtagatagac
    10921 ttcccaaagg atgattgcaa gcacaatcaa tagcaaaatg atattaatta cccataaaac
    10981 catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta atgaggcagc accaatgaca
    11041 ccagcttcat taccaagctc tgctaatttg atttttgttg attctcttac ttgcgggaaa
    11101 gtaaattctt taaaatattt ctctacacgg ctacgtaaaa attctcctgc tgccgatact
    11161 cctccaccta acacgatact tgacggattt aatgtattcc ccaagtttcc gcaagctaaa
    11221 cctaagtaga agcatacgcg atcaacaacc atcaatgcaa acggatcgtc ttcttgagcc
    11281 atttcaaaga tatctttact tgttacttct tctccgttgt ctagcattgc tttcagtttt
    11341 gaatcccctg cgtattcttc tgccaagaaa cgacctaggc gtaccacacc tgtagcgcta
    11401 gatacagttt ctaaacatcc gcgtttaccg caagtacatt cgaacccttc aggatccaca
    11461 gtgatatgtc cgatttctcc tccagctccg ctcacgccgt gaatcaaatt tccgccagca
    11521 acgatacctc cgccaacacc tgttcctaga gtaacgaata caacgtctgg atcgttttct
    11581 ccagcacctt tccatcgttc acctaaagca gcaacattcg cgtcattatc gatagcgaat
    11641 ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt gaacttctgt ccagtttaag
    11701 ttatacgcac caataaccgt tccagcttcg ctgtccacgc ttcctggggt acccatgccg
    11761 attccgataa agtcttcggc tttcatatta tataaattca aacgatgatt gattgattca
    11821 atgatttcag gaacgatatg cttaccatca tccaaaatat tggtatcgat gctccatttt
    11881 tgctgaatct ctccttctgg tgttaaaatt gcaaattttg ctgttgttcc acctaaatca
    11941 atcccaataa tcttcttgtc cattacaaat ttcctttctt tcggctttct tcaattcggt
    12001 gctctctttt taatatgcgc cacgctgtca tgtatgtatc atgatcaatc aaacggctat
    12061 catacagatt acgcaattcg atagtcatta attcaatgtc atagatgcgt gcacccacat
    12121 agataaagat gccaaatttt ttgaacagct gctgtacatc atataagctc tccatcttta
    12181 agcacttcct tcatattata caagtaaacc atattttttt aaacccagta aaatacatat
    12241 tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt tggtgtatcg aatagcttgt
    12301 ctgttgtttc ggtagaccca aaacattccc taacactaat ccgccaatta gcccaccgac
    12361 atgtccccaa acatctacag aggtatccag aagaccaaaa acaaatgtca acacaataaa
    12421 taaaagaaaa tgtctgacta gctgctggat cgctgggtta tgtttaaaat gaaaccctaa
    12481 gataaacaac gcaccgaaca aaccgaatat cgaagtactt gcaccaccag acagaacatt
    12541 cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc ccgctgaaca tataaatcaa
    12601 gaaaaaacgc caatggccat agattgcctc gatttgctgc cccataaaat acagtatcag
    12661 tgagttcatt acgaaatgca tcagtccaaa atggatgaag attggtgtca ctaaacgcca
    12721 ccattcattg aagtgaacaa gataaggacc atacattccg gcataataag ggatctcaag
    12781 tcgcggaaat aattccatca tgaggaagac cgctgtctgt atccctaaaa aagcataggt
    12841 ccataacggt cgtcttaacc atgcacgcaa ttttatttgc tgttgataat tcatgaaaac
    12901 ctcccatttc attttagtca ataaacaatt gttggatctt ctgatcaaag ctctccggtt
    12961 gccattgttc attcaattgt tcacgaaaaa caaggctaca tgttttttta tgaaaattat
    13021 ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg ataaccggct ctattgtaga
    13081 taatacctgg tacaatgagg agatcgatca tctccggcaa atagagcttc tctgattgtg
    13141 gctcaagaac accaaaactc gaacgtacaa gaacggactg ttcatccatt tcataaaacg
    13201 ccatctgacg tttcggcaaa gttttaggta caactatttt cttttgttct ttttctgctc
    13261 gttggataac cggctgtgta tccagttcaa attcattcga taaagtcgtc cctatcactt
    13321 ttgctgactt ccagtactcg ctggcgaata gctgtttata gattcgctgt tctttttcct
    13381 ttttcttgcc acttttggcc agatttttca gcttttcgat tcctttccgg cgtaatctta
    13441 ctttcaattg gtccaaaaac tcacccttcc ttctctcata aaatggtata ggaaaaaaag
    13501 aaaaatagca atcgaacttt caaataaatg cataaataga ataaaaagaa gacctagaaa
    13561 tgattggcct tcctttttta atgaatttca actgttcagc gcgtgttttt gtctgataaa
    13621 aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc aaaattcctg taggtacttc
    13681 cccacttggt agaatcactc tagcaaccat atctgcacta agcagaaaaa tggaaccgaa
    13741 caaaccgctc agaacaaagg tccagttatt ttctttacgt acgactagct tcgcagcatg
    13801 aggagctagt agtcctacaa aagataaact acctgtcacc gctacactac cgcttgccaa
    13861 agcaacagct aacaacaaaa ggattttttg acttttagcc acgttaaggc ctaatccttg
    13921 cgcgcgttct tgaccaaatg atagcaatac aatcctctgt gcataaagtc ctcctagcgg
    13981 aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg caccccagat
    14041 tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt ggatcgtgca
    14101 tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata agattcgatt
    14161 catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag cagctaaaac
    14221 accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg aaaagaaacc
    14281 taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac tagaatctgc
    14341 caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac aagctcctgc
    14401 taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg ccaaagaaga
    14461 tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag gcgtaccaat
    14521 cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataga acgtccaata
    14581 tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac gccaatcaat
    14641 gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc tgtaaaatcc
    14701 gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata acgatagtcc
    14761 gttccaacca ttccgcgaac aactggcgga accaacagcc caataaatga aacaggtccg
    14821 actaacgaaa cggcaatggc tgctaacact gcaactagac agaaaacaat caaccgcgtt
    14881 cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat caaagatatt
    14941 gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa caatactgct
    15001 aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag atcctgctgc
    15061 agattaaaga attgggtaag tgcttggctg atagcagtaa aaaaagaact aatagctacg
    15121 ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa aaaaccaaat
    15181 acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaacacaga aaaatcagaa
    15241 gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc gttgattcct
    15301 agtaagcctg aatctgctaa aggatttctt gtcacccctt gcatcaatgc accgctcaat
    15361 gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat atttcgtatg
    15421 atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa atctgctttt
    15481 tccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag aacaaaacag
    15541 accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc tgctcttttg
    15601 gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg acaatcgttg
    15661 catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca ggtgtacctt
    15721 ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt gaagcatgat
    15781 taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt tcagtcaata
    15841 actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca tccaagatca
    15901 agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt tgtccaccag
    15961 acagcgttgc gatatcccgc atacgaaatc cagctagacc tgtagcatcc aacgcccact
    16021 caattgcttc atgatcttgc gtgtccatgc cactgaacag tttttgataa gggtatcgtc
    16081 cgtaagatac tacttcttct actgtcatac ctgtagggtg ttcactagtt tgtgctaata
    16141 atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga gatatcagag ttattgacaa
    16201 tgatctttcc tgatttcaca ggtagtatac gggtaaaact tttcaataaa gtagattttc
    16261 cacttccatt tggcccaatc aaactagtga ttttcccttc aggaatcgta acagacatgt
    16321 tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc agctgctctt aatctcgtca
    16381 tttcacccat cctttcgctg aaaatcattc tcaattatga cgctgtttca acatttcgtc
    16441 aagcgctact ttcatatcta tagccaaatg aagcggttga cagcgttcga aatatcagga
    16501 ataataacca atgagaaaga ttctcaatta tgttaggagg aaagaaatga aattaaaaac
    16561 aaaagtattc acattatcta gcatattgct gctggctgga gtactgagcg cttgctcccc
    16621 ctcttcagat aaagaaacag ctacaactgc caattcgacg acttcatcca atagtactca
    16681 tacagtcaca gatacacttg gacataaagt agagattcca aacgcgccca aacggatcat
    16741 cggcagttac ttagaagact atctgattgc actaggggaa aaacctatcg cccagtggac
    16801 tgttggcagt ggttccattc aagattatct gcaagatgat ttaaaggatg tcccaacgat
    16861 ttcttatgac cttccttatg agaaagtgtt aagctttgag cctgatttgc tgttgatttc
    16921 ttcttccgct actgtcgaag gcgggaaata cgaacaatat agcaaaatcg cgccaactta
    16981 tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa ctaaaagatg tcggcaaagc
    17041 tttggcaaaa gaaaaagagg cagaaaaaat catttccgat tatcaaaaat ccatcaagaa
    17101 aactcgagaa gaactagcag ataaaatcga gaacaagaca gctgcagtgt tgtgggtgac
    17161 caacaactca gcctttatgg tcagcaaaaa tcggtcaagt ggaagaatcg tatacgacga
    17221 tctggaattt ggtgttcctg atctagtgga agaggtctcc aaaaaagcca cttctgactg
    17281 gtctgctgtt tcctctgaaa agctagctga attagatgct gactacatca tccttgtgaa
    17341 cagtgacaaa gatgctgcca tgttcaatga accaagctgg aagaatctaa aagcagtgac
    17401 agacaatcat gtactgcaat tcggacctga atctagttgg ctatataacg gacctattgc
    17461 cagtcaggaa atggtcaatg atatcaaaga aaaactaaat tgattttaac taaaaaaagc
    17521 atcttcacga ttagtcgtga gatgcttttt ttcttattaa caaactagac gcatagattt
    17581 tattcttctt ttgaagcaac cctatttttt actctttccc atttttgaaa acgatgggca
    17641 tatagatttt cttgatccgt tatcccctca tactcctcta taagggcgaa gtctgaaaaa
    17701 tcaatattcc caatatacgt atcgccttca aacgtgctat cgatcaatgt tcgccagatc
    17761 cgaccagtgt aaggaagaag ttcttgaaaa atccgactac cacccgaaac atagatatct
    17821 tctgagatcg tttgactata cgacaaaagt tcatcgatcg aatgcattac ttccgcattt
    17881 tctttcgtgc gaaaatttgg ttgcgttgtc aacacgatga tatggcgata aggaagtgaa
    17941 agatccccca ttccttcata ggtctttctt cccatcacta aaattcgatc cattgtatgt
    18001 tcgcgaaaga aacgcatatc atttggcaat cgccatggta acaaaccatt tttgccgatc
    18061 agaccatttt tatcttgtgc ccacatggaa ataaacatat tatccgctcc ttatgatttt
    18121 agatgttttg agataaaaaa agtctcaatt ctttgaccag caaggaattg agaactttaa
    18181 aaacattatt ttgtttcacg gtgcaaagta actttgcgtt cgcgtgggca atatttttgt
    18241 ttttccaaac gatcaggatt gttacgtttg tttttgcttg ttagatagtt acgttcttta
    18301 caagaagtac actctaaagt gatgtttacg cgcatgtttt tccctcccat ttttacaaga
    18361 atcctctaag atgactctca gacttgatta tcttaccatg attttttcta gattgctagt
    18421 gtttttcacg attttgttaa gtaaaactac ttgacgataa atacaacggt tttacttctc
    18481 aaacaaaatc tttttctttt tgtataaata cccttacact tgtttatata gattatgttt
    18541 tttaataaag ggggatacta atatgccatg gaacatgaag gactttcctg catcaatgaa
    18601 aaacttagat aaattgacaa gaaaaaaagc aatcgacatc gcaaatgcat tattagatga
    18661 agggtaccct gataaccgtg cgatcccaat tgccattgat caagcaaaaa aatggtatga
    18721 agatgctagt gaatcagaaa aacgaacttt cgaaaaagaa aaaaatccaa gcaagacgga
    18781 tgaacacgat acgaatcctc gtgctggaaa actgcttgat tcagatgtga tcgtagaata
    18841 tgaggaagaa caatggatcg tgaaatctaa aggggcaaaa aaggcaagca accattttga
    18901 tacaaaaaaa gaagcaatcg aaaaaggaaa gcaagtagca caaaacaaag aatcaacttt
    18961 gttcatttat aaaaaagatg ggacaaaaga aaaagaaatc gattattgaa catatgtaaa
    19021 ggggttgtga cgaaaatcgt gtcacaaccc ccttatgttc gaaacaatgg tgttcctctg
    19081 ccaactccga accataagtc ataatatcta taaaacggga agagctgttt tttgaaattg
    19141 tttcttatca ctcagtgtta aacggtagga gaaaacctct tttttattga tatttgattt
    19201 tcatcaaaca gtcacatagt cgattgcttc tgcttgcgga atcgtttcat ctacaaagta
    19261 gactttatac catgtcaaga aactgaagat tgtccagatt tttctttgct cttctttacg
    19321 tccttcgtga tggtcatcta gcaacttcat gattttttct tgatcaaaaa attcttttgc
    19381 aaaatctgct tcgaataatg ctttgacttg ttcgtagccg tgttcttctt tcaaccaagc
    19441 tttgattggc acagggaatc ctaatttcac acgattcgac cattcttccg gaagatgttt
    19501 gttcgcagct tgtctgaaaa tatcttttgt gttgttttga ttcaataaaa acttacttgg
    19561 tatttgttga gccaatttca tgacttcgat atccaataaa ggtactcgga cttccaaaga
    19621 acttgccata gaaagtttat ctgcctttaa caagatatct tttggcatcc attgatgaag
    19681 atcgacatat tgcatcttat tgacttcatc tttgattcct tctgttttct cgtaatgaac
    19741 agtcatgatt tcatccacag atggtgctgt ttgatatttt ggtgttaaca cttctgatgc
    19801 ttctgattct tcgaacactc gagcatgacc gataaaatat tcttttgcag gagcaagtga
    19861 ttcatataaa tggatccgtc catggaagtt tttcattttt ccgattttac gtcctaaatt
    19921 atagcgtgtt ccttttggta attttttcaa tccttgagca atcacacgga tgaattttga
    19981 attcgtatgg aatccatacg tttggtatcc cgcaaacagt tcatccgccc cttctccaga
    20041 ttggactacc cgcacacttt gtgaagcaag atttgctaaa aagtacaaag gtacgcaaga
    20101 aggatttgaa tcaggttcat ctaagtgata ctggatcaaa gggaatgctt tgaacgacat
    20161 gtctccgtcg atcactgctg ctgtattatc taagtccaat agttcggtta gttttctggc
    20221 ttcaatcgct tcattatagg ttttatcatc aaacccgatt gagaaagaat gatctggttt
    20281 caatacagag gttacataac tggaatcaac cccgctggat aagaaagaac caacttctac
    20341 atcactgacc gtatgtgctt caatagaagc ttgaacggtt tcgtcgattt tttcgatcca
    20401 ttcttgtcgg gaatgcgttt cttttgtttc aaaatctgca tcccaatatt gctgaatgtc
    20461 tatttttcca tctttatacg tataataatg gccttcaggc aaacgataga catctttgaa
    20521 gaatgtttcg ccgtttaatg gagaatattg gaaagtcata taaggtttca acgcttcttt
    20581 gttcaattct ttgttaaaat caggatgagg cagaaaactt ttgatttctg aaccaaacat
    20641 aaatgttccg ttcatttctg cataataata tggtttgatc ccaaagtggt ctcttgcacc
    20701 gaataactca ttcttttcat tatcccaaat agcaaaagca aacattccgc ggatcttttg
    20761 caataattct gttccccatt cttcgtaacc atgcaaaagg acttctgtat ctgcatgtgt
    20821 ttgaaaaacg tggccagctg caatcaattc ttctctcaaa ggctggtaat tatagatttc
    20881 gccattaaaa ataatgatct ttgtccgatc ttcattgtaa ataggctgag taccgccctc
    20941 taagtcaata atacttaatc ttctgaatcc taaagcaaca tgtttatcga tatattctcc
    21001 tgaactatta ggtccacggt gaacgattcg atccatcatt gtattaataa ttgttttttt
    21061 gttgtccttg tcgttaacaa aacctacaat accgcacatt taactttcac atcctaatat
    21121 aaaattactg tttatttcat tcatatgcat cttatcatag aataaatagc ggacgaatat
    21181 tttctctctt acaatttcgt cacgtgaaaa caaaagatga aagtttctgt ttttacagaa
    21241 aataaagtcc caatatctaa tttaccatgt tttctttttt gaggctacct tattctcttc
    21301 gtgttaaaaa aatattagtg gatgctattt ccaaaaaaac cagcaaaaaa agacacgacc
    21361 taaagatcgt gt
//
LOCUS       DASJPC010000043        21059 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJPC010000043 DASJPC010000000
VERSION     DASJPC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.043"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            37..1203
                     /locus_tag="U4A13_001572"
     CDS             37..1203
                     /locus_tag="U4A13_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1779872.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            1433..2080
                     /locus_tag="U4A13_001573"
     CDS             1433..2080
                     /locus_tag="U4A13_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN1779873.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            2164..3108
                     /locus_tag="U4A13_001574"
     CDS             2164..3108
                     /locus_tag="U4A13_001574"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN1779874.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            3243..4694
                     /locus_tag="U4A13_001575"
     CDS             3243..4694
                     /locus_tag="U4A13_001575"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN1779875.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            4715..5551
                     /locus_tag="U4A13_001576"
     CDS             4715..5551
                     /locus_tag="U4A13_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1779876.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            5551..6210
                     /locus_tag="U4A13_001577"
     CDS             5551..6210
                     /locus_tag="U4A13_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1779877.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            6336..7025
                     /locus_tag="U4A13_001578"
     CDS             6336..7025
                     /locus_tag="U4A13_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779878.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            7031..7987
                     /locus_tag="U4A13_001579"
     CDS             7031..7987
                     /locus_tag="U4A13_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN1779879.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            8031..8543
                     /locus_tag="U4A13_001580"
     CDS             8031..8543
                     /locus_tag="U4A13_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1779880.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(8799..9347)
                     /locus_tag="U4A13_001581"
     CDS             complement(8799..9347)
                     /locus_tag="U4A13_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1779881.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(9436..10617)
                     /locus_tag="U4A13_001582"
     CDS             complement(9436..10617)
                     /locus_tag="U4A13_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN1779882.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(10853..12361)
                     /locus_tag="U4A13_001583"
     CDS             complement(10853..12361)
                     /locus_tag="U4A13_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN1779883.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            12520..12711
                     /locus_tag="U4A13_001584"
     CDS             12520..12711
                     /locus_tag="U4A13_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779884.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            12914..13831
                     /locus_tag="U4A13_001585"
     CDS             12914..13831
                     /locus_tag="U4A13_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN1779885.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            13824..14897
                     /locus_tag="U4A13_001586"
     CDS             13824..14897
                     /locus_tag="U4A13_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779886.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            15045..15563
                     /locus_tag="U4A13_001587"
     CDS             15045..15563
                     /locus_tag="U4A13_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779887.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            15544..15936
                     /locus_tag="U4A13_001588"
     CDS             15544..15936
                     /locus_tag="U4A13_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN1779888.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            16012..16947
                     /locus_tag="U4A13_001589"
     CDS             16012..16947
                     /locus_tag="U4A13_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN1779889.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            17080..17859
                     /locus_tag="U4A13_001590"
     CDS             17080..17859
                     /locus_tag="U4A13_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN1779890.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            17852..18169
                     /locus_tag="U4A13_001591"
     CDS             17852..18169
                     /locus_tag="U4A13_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN1779891.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            18413..20023
                     /locus_tag="U4A13_001592"
     CDS             18413..20023
                     /locus_tag="U4A13_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1779892.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            20180..21028
                     /locus_tag="U4A13_001593"
     CDS             20180..21028
                     /locus_tag="U4A13_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1779893.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6849 a         3746 c         4215 g         6249 t
ORIGIN      
        1 taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac tttatacaat
       61 caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga cggtacaacg
      121 aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca tgaagaaatc
      181 ccaatgaaag agattacaag taacgcatct ttggcactag gagaagccta tatggatcat
      241 cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca gcaagcaaac
      301 agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa acatacgaaa
      361 caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt ttataaactt
      421 tggctagatc caactttgac ttattcttgt gcttatttca atacacctga agatacgttg
      481 gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat caaagaaggc
      541 gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc taccaaagag
      601 tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt gattaccaaa
      661 agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga ttaccgcgaa
      721 ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca tgtaggttct
      781 gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa aggaacagct
      841 ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat caataaatat
      901 attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat tacaaaaaac
      961 gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac attagaacat
     1021 tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg tgattccttc
     1081 tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc aagtaatatc
     1141 gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat gcatcgtgca
     1201 taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt ttatgatgct
     1261 ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag tttctttttc
     1321 tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag aaaatttttt
     1381 cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat caatgaaaaa
     1441 aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc aatcaaatac
     1501 caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt cacaggtatc
     1561 ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt ctagtgaagc
     1621 aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt tccaagataa
     1681 atacccagat actgcaatta ctagtttgga attagatagt gattggggat cttacttcta
     1741 caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg ctcaaaatga
     1801 aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg gtcaaaagaa
     1861 aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag caaaaatcgc
     1921 cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag atttaggtac
     1981 tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca agatcaatag
     2041 ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc ggaaaacaag
     2101 agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga aggagcgatt
     2161 tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt gatgaatgca
     2221 tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc agaagcagga
     2281 gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga accacgctat
     2341 tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg tttttcttat
     2401 tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca accgttattt
     2461 ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa aatagaaaaa
     2521 agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc tggaaaacca
     2581 caactagctt acgattttga ggccacttat gaaacactga aagaagtatt cagcatcttt
     2641 tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt tgatcagatg
     2701 gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag tgtgggcaat
     2761 ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga agggttcggc
     2821 ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc tttttataca
     2881 cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg acaagatgca
     2941 tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtaccgagct tcataaagaa
     3001 gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc agaaaaagga
     3061 tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca aagagacgaa
     3121 ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat tcctgtgcaa
     3181 gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa aaggaggata
     3241 atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt agttttcaag
     3301 aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg aatgcgtttg
     3361 tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag tcagaaaaag
     3421 ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa aagggatggc
     3481 tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca tctaattatg
     3541 tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg ccagagtttg
     3601 gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct tggaaccttg
     3661 aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca ggaatcgttc
     3721 ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg ttttgcggat
     3781 tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct tggagaggat
     3841 ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg gaggctttat
     3901 tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca gttgagtggc
     3961 aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca ccgatccact
     4021 cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc cttggaaaag
     4081 aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg atcacgtctt
     4141 attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa acaggcatcc
     4201 agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc caatatggtc
     4261 agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa gcagcaattg
     4321 tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact gcatttaccg
     4381 cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg gaacacatca
     4441 gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag gagagcttga
     4501 agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc agcttgccta
     4561 ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc agaggaagag
     4621 aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc catttaccaa
     4681 atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat tgtttgctct
     4741 aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta atcaggaaga
     4801 taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt ctggcacact
     4861 gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg tagatttggc
     4921 aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca acgtggatgg
     4981 acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt ttggtatcac
     5041 aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat ttgacagtat
     5101 gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta aagggaaaaa
     5161 agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg gagctaaatt
     5221 agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa atggtcgaac
     5281 agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt taccagatat
     5341 ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt ttttattgaa
     5401 agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga tgaaagaaga
     5461 tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag tcaaaccgaa
     5521 agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ccctaattga tttttccctc
     5581 atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg gattggcttc
     5641 gtgtcgtttc ttttaggaaa tttggtaggg ttgcttttga cagttttagg tcttttggat
     5701 tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg cattcccgca
     5761 ctagtcctgc tgtttctgtt atatttcggg ttaccttatc aattaagtgc cttaacggct
     5821 tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta tcgaggatca
     5881 ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt ttctttcttt
     5941 ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc ggcactcagt
     6001 aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac agtgccagaa
     6061 atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc tctctatatt
     6121 ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca acagtattta
     6181 gaaaaacgtt ctatccatca gtcaatctga aaaatagaaa tggtgctagc gtggctggca
     6241 ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca tttcatgtaa
     6301 aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta accatcgaaa
     6361 tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta gaagaagtca
     6421 cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa tttgatggaa
     6481 gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc tctacagcca
     6541 aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta tatgaagaag
     6601 taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac acggttgaag
     6661 aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag aaagagcttg
     6721 ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg gaagtaagaa
     6781 aagcaaagca aatggagcga ggcgtaaaat attttccaaa agatcaattg tacaaagaat
     6841 tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat gaacggtttt
     6901 tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc gcgcttcagc
     6961 atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca gagccattgg
     7021 agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat tagcattaaa
     7081 agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg aaccagtacg
     7141 tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc atccaatcgc
     7201 tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg tgcacagttg
     7261 gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag aaaatctcag
     7321 tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact acccaaaaga
     7381 tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac cagatactag
     7441 tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt ttcaaacgag
     7501 aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg actatctttt
     7561 tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta gccgttattt
     7621 cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg atgcttacgg
     7681 caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag accaagcatt
     7741 gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc atcatcaact
     7801 cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa aattccctgg
     7861 actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta gtcacctgct
     7921 agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat atgaagtgat
     7981 ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg atgtatattc
     8041 ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc gcagcggatt
     8101 tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca aaagaaacga
     8161 ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat caaattgctg
     8221 gaacagctgc attagtctca ggcgtggatc ccgtttacac aaaaatagat ggtagttgga
     8281 aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa agtactcgag
     8341 gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc agcaaaaata
     8401 tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag accatctatt
     8461 ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc gaaagaaaag
     8521 catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa gacattaaca
     8581 ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg ccagcttcaa
     8641 aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt agcgtatttt
     8701 acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag cttttgtcac
     8761 gatctctcgg cttatcctac gagtattatt cgatacattt atatcaataa tccttttttt
     8821 attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag tgaggggata
     8881 gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct ggctgaaatc
     8941 tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt agagatcgta
     9001 ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa accatttgct
     9061 gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc tttactggaa
     9121 ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt gatcatctga
     9181 ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc aggtcttggt
     9241 gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc tgcgagtgaa
     9301 gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt catttctcct
     9361 ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt aaaagtaact
     9421 ttgctttttt gtattttata ccagcaagcc agtcacaata gcacttaaaa agctgactaa
     9481 agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt tttcttcatt
     9541 taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa atgaaacgag
     9601 gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat ttgctaattc
     9661 tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac cggcatccac
     9721 tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt aacctaggat
     9781 ctcttggaaa gtgatgccga atatcccgtg gaagatagca ttgatcatgg caatcaaagc
     9841 aacaaaaccg ataagcatag cagcaaccgt gatcgccaca tggaaaccat caagaatgta
     9901 ttctcccaac atttggaaaa atgtctgttc tttgttttct tcaatgatca gctcatcttc
     9961 tttttcttct aatacatagg gatttacaat tgaagccaga atataaccgc caaataagtt
    10021 caaaacaagg gcagtgatca catatttaga atcgatcagt gccatataag atccgacgat
    10081 cgacatagaa actgtagaca tagccgatac actcatggtg aacaaacgtt tctcagatag
    10141 gaagggtaat tcctttttta tggaaataaa aacttccgat tgtcctaaaa tagcagaagc
    10201 tactccatta taagattcca atttgcccat cccgttgatt ttacttaatc ctagaccaac
    10261 agctttcata aataatggaa gaatcttgat atagcgtaga ataccgataa tagcagaaat
    10321 aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca cttcctttat ttgctacacc
    10381 ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga agatgatcaa acatttttgc
    10441 caatacagaa acaaccgctg taccaaaagt tgtacgtaat aatacgaaac caagaacaag
    10501 ctgtaaaacg aacatcacaa ttagtggttt caatttgatt gcttttttgt tactgctgat
    10561 caaaaaagca agccctaatg tgacaaataa accaataata cctaataaat aactcaaata
    10621 atcactccta ttccaaattc cgttttattt tacctgtttt gcaaataaaa tactaatctt
    10681 tagtgaaatt ttcattactt ttttagagtg aaacgcattc attttccatt agaaaataaa
    10741 gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt aaagtctata aaaaaacgaa
    10801 actatcgatt aagctaatag tttcgttttc aacatttata agtaaacttt ttctattggt
    10861 aatatagatt ttcggaaggg tagacaggtg cgcaggtaat atcgattcct agttttttca
    10921 atgtttgttc atcattttta ttgaccatca cagtagagtg ggcttgtgct ccctcaagtt
    10981 cagctagttt atcataagca acttgagcga ttggattcgt tactgcactg attgataaag
    11041 cgatcaagac ttcattgatg cttagtgcat ccaaagagga atgaagtgtc tttgatttca
    11101 atccttgtat tgtttcaaga actaatggtg acaataaagg aatgtcatct gcgatatggg
    11161 ctaaatgttt tactgcattt aagataactg ccgctgacgc atccatcaat gaacttgtac
    11221 gcccggtaat gatgacttca tctggtaatt cgattgcaat cacagcagaa ggatcttttg
    11281 taggactttg cgcttgaatc ttatctgcat agtcatgggc agggactacc acgtttcggt
    11341 cttcaggacg taaaccgact tcttccataa tcagtttgat tcgattaact gcttcttcgt
    11401 cgatcaatcc ttttttgaag tcgcattctg tttggaaata acgacggatg atttcttgtt
    11461 tagaggcttc ttggacaacg gtatcatcca cgatcccaaa tcctacacga ttcacaccca
    11521 tgtcagtagg agattgataa acagattctt taccagtaat tttttcaata atacgtttga
    11581 ttacaggaaa tgtctcaaca tcccgattat agttcacagc tactttattg taggcatcaa
    11641 agtggaagga atcgatcata ttgacatctt ttagatcgac tgtggctgct tcataagcaa
    11701 tgttcagcgg atgtttcaac ggaacattcc atactgggaa agtttcgaac ttggagtaac
    11761 cagctgcatg tcctttttgg ctttcatgat acagttggtt caagcaagta gcgagctttc
    11821 cacttcctgg gccaggagct gtcactacaa cgattggttt agttgtttca atatactcat
    11881 tttttccaaa accatcttct gagacgattt tttctacgtt gactggatag tcttcgattt
    11941 ctgagtgttt atagaccttg atgtttcgtc tttctagttt attgataaag actttcgtgg
    12001 caggctgtcc actatagcga gtgataacga cactgttgat taataagcca taaccacgta
    12061 attcatcgat taaccgcaag atatccatat cataagtaat cccataatca ccacggatct
    12121 tattacgttc aatatctcca gcatagacgc aaatcagtat ttcagcttga tctttcaatt
    12181 tttgcagcaa tttgattttc gcatcttctt caaatcctgg aagtacacgt ttcgcatgct
    12241 tgtcgtcaac taacttccca ccaaattcta aatataattt gtcatagtgg ttcactcgtt
    12301 ccaaaatata cgctgactgc tcttcaatat atttttctga atcaaaacca atttttttca
    12361 ctaggttccc tccttaagta ctaaagcatt ttttcatttt tttgaggatt cgtcaactgg
    12421 aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa accaacttgt ctagatagtt
    12481 attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag acagaaaaag
    12541 aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag aatgcttgaa
    12601 gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca gctaactcgt
    12661 gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata aaaaataaga
    12721 taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga tcgaaaaaaa
    12781 ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct tttttataac
    12841 aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag atgagtaaga
    12901 ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag acgaactaga
    12961 ggagacgaac gcggatcagg atacaggaca agaatcgtct tctttttacg atatcaaaga
    13021 aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg aaaaagctgt
    13081 ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga atgaagaatt
    13141 aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta agatccatga
    13201 tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc caacgatgag
    13261 ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg atgcaagtct
    13321 gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt cccaaccgga
    13381 gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca ctgctcagac
    13441 agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga ctgatttgaa
    13501 cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag aattagagaa
    13561 gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg agacgcctga
    13621 aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc agcttcgtgc
    13681 agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca aagagggatt
    13741 cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc ctaataatca
    13801 atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg acaaaaaatg
    13861 aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa acaagtaatc
    13921 ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacat gaaaaagaaa
    13981 aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa ataactgaaa
    14041 atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt ttgattgaaa
    14101 cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa tcagattttc
    14161 cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc tccacgatcg
    14221 gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg aatgatattg
    14281 gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt tcgatcaatt
    14341 atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc atcgatcaac
    14401 gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg gaaatcaatg
    14461 tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga gtattctctg
    14521 cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat gcaagaacac
    14581 ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt ttgaatcttc
    14641 agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg gcaatgaaaa
    14701 atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga ttagataaag
    14761 gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg atctcatctg
    14821 gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg gtcccattaa
    14881 tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga taattctgtt
    14941 ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg tatgtactaa
    15001 atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc acaaataaaa
    15061 tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct tttcaattta
    15121 ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac ttaagcttaa
    15181 aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat tttggtgttg
    15241 gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg ttttcaatcg
    15301 tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt ttttttctgg
    15361 cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat cgtttgcctc
    15421 aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt ataggcaatg
    15481 ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat tcaggaggaa
    15541 aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact cattttgaca
    15601 agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt aaaaacatcg
    15661 caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa agaagtggtc
    15721 ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt caaagaagat
    15781 aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca actctattgg
    15841 atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa attgaatgat
    15901 ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac tgttaaaggg
    15961 cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa catggaaaat
    16021 tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat tctagttgct
    16081 atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa cgaaagcatc
    16141 cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa acaagccaat
    16201 aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt tttcagtaca
    16261 atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga agcttttgaa
    16321 aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc aatactgtta
    16381 tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat caaagaattg
    16441 aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag tgaaatactg
    16501 attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt agcaggaaca
    16561 atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt gctgatcggt
    16621 ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct gatcatcggt
    16681 gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg tcctgagatc
    16741 agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt agttgcagca
    16801 aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa tgatattgaa
    16861 aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga aacagataaa
    16921 tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc aaaaatgaga
    16981 aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat gttacaatga
    17041 gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa gaaaagtgcg
    17101 ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat accagtagga
    17161 ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc aggactgtta
    17221 tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct tacgacgcaa
    17281 gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta tacattatta
    17341 ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc tgtcttttct
    17401 caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac tgacccttcc
    17461 tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg ggccggtagc
    17521 aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct tgtccatttt
    17581 gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc actactgatc
    17641 tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt ccaaaatact
    17701 ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct ggagaaggca
    17761 ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa tgagccgctc
    17821 ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc tacttttagt
    17881 tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt attttactca
    17941 tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg cattatttgc
    18001 ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg cttggaatat
    18061 caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt cgatggcgtt
    18121 gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa ctgttttatt
    18181 ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga actgcatttg
    18241 caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag tgttggctta
    18301 tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct atgatttttt
    18361 gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt aaatgaataa
    18421 aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta aagttttagg
    18481 ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg gtatgcttgc
    18541 tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc ttggtacagt
    18601 cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag gagataacgc
    18661 tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg gtatcgcctc
    18721 tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga ttgctaacgt
    18781 taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta tcccaattct
    18841 tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg gaacttcttt
    18901 gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt acttgcgcgt
    18961 attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt ctttcttcgg
    19021 tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact tcttcgaact
    19081 taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg cctctgtctc
    19141 tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta tcgttcaaat
    19201 gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga tcgcagatca
    19261 gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga cattgatcct
    19321 gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca cgatgaagaa
    19381 aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg cgttattgat
    19441 tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca cattgttttt
    19501 tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt ctttgttttt
    19561 ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc gttttgcgat
    19621 ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg gattaggatt
    19681 agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg tctttgttcg
    19741 cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga tcagcaattt
    19801 tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg ccgttttctt
    19861 tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt attgtgtggc
    19921 tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa atgggtttcg
    19981 aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact aatcgttttt
    20041 tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc tattttccaa
    20101 ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa aaattctttg
    20161 aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg catatctgcc
    20221 ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga ttatggatgg
    20281 gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac ttttaaaaaa
    20341 cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg gtttatatga
    20401 ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt cagggatttc
    20461 tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa cggtcattcc
    20521 ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg ccttagactt
    20581 aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa tccgagataa
    20641 tacctatgct tatctgattg tagatgattt gaacaattta gtacgaggag gacgtttgac
    20701 aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga cattttctga
    20761 agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg ctcgagctga
    20821 agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt acgtgataca
    20881 tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa aatatccaaa
    20941 tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag gtgaaaaagc
    21001 catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata ccaaaaata
//
LOCUS       DASJPC010000044        20955 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJPC010000044 DASJPC010000000
VERSION     DASJPC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.044"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            591..1280
                     /locus_tag="U4A13_001594"
     CDS             591..1280
                     /locus_tag="U4A13_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1779894.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="U4A13_001595"
     CDS             1267..2475
                     /locus_tag="U4A13_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1779895.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="U4A13_001596"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="U4A13_001596"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN1779896.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4A13_001597"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4A13_001597"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN1779897.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="U4A13_001598"
     CDS             complement(6578..7273)
                     /locus_tag="U4A13_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN1779898.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4A13_001599"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4A13_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN1779899.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="U4A13_001600"
     CDS             complement(8472..8663)
                     /locus_tag="U4A13_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN1779900.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="U4A13_001601"
     CDS             complement(8684..9574)
                     /locus_tag="U4A13_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN1779901.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="U4A13_001602"
     CDS             complement(9561..10328)
                     /locus_tag="U4A13_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1779902.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4A13_001603"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4A13_001603"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN1779903.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4A13_001604"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4A13_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN1779904.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4A13_001605"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4A13_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN1779905.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="U4A13_001606"
     CDS             14899..15282
                     /locus_tag="U4A13_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779906.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="U4A13_001607"
     CDS             complement(15450..16208)
                     /locus_tag="U4A13_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN1779907.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="U4A13_001608"
     CDS             complement(16226..17044)
                     /locus_tag="U4A13_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN1779908.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4A13_001609"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4A13_001609"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN1779909.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4A13_001610"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4A13_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN1779910.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="U4A13_001611"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="U4A13_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN1779911.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="U4A13_001612"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="U4A13_001612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN1779912.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5991 a         4264 c         3837 g         6863 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac ctttt
//
LOCUS       DASJPC010000045        20747 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJPC010000045 DASJPC010000000
VERSION     DASJPC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.045"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(199..1182)
                     /locus_tag="U4A13_001613"
     CDS             complement(199..1182)
                     /locus_tag="U4A13_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN1779913.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1187..1834)
                     /locus_tag="U4A13_001614"
     CDS             complement(1187..1834)
                     /locus_tag="U4A13_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1779914.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1919..2551)
                     /locus_tag="U4A13_001615"
     CDS             complement(1919..2551)
                     /locus_tag="U4A13_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1779915.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2567..3118)
                     /gene="coaC"
                     /locus_tag="U4A13_001616"
     CDS             complement(2567..3118)
                     /gene="coaC"
                     /locus_tag="U4A13_001616"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN1779916.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3115..3852)
                     /locus_tag="U4A13_001617"
     CDS             complement(3115..3852)
                     /locus_tag="U4A13_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN1779917.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3849..4838)
                     /locus_tag="U4A13_001618"
     CDS             complement(3849..4838)
                     /locus_tag="U4A13_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1779918.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            5096..5638
                     /locus_tag="U4A13_001619"
     CDS             5096..5638
                     /locus_tag="U4A13_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779919.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5655..6740
                     /locus_tag="U4A13_001620"
     CDS             5655..6740
                     /locus_tag="U4A13_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1779920.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6752..7558
                     /locus_tag="U4A13_001621"
     CDS             6752..7558
                     /locus_tag="U4A13_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779921.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7559..8386
                     /locus_tag="U4A13_001622"
     CDS             7559..8386
                     /locus_tag="U4A13_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1779922.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8383..9456
                     /locus_tag="U4A13_001623"
     CDS             8383..9456
                     /locus_tag="U4A13_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1779923.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9884..12580
                     /locus_tag="U4A13_001624"
     CDS             9884..12580
                     /locus_tag="U4A13_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1779924.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12657..13145
                     /locus_tag="U4A13_001625"
     CDS             12657..13145
                     /locus_tag="U4A13_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1779925.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            13157..13651
                     /locus_tag="U4A13_001626"
     CDS             13157..13651
                     /locus_tag="U4A13_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1779926.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13672..14481
                     /locus_tag="U4A13_001627"
     CDS             13672..14481
                     /locus_tag="U4A13_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1779927.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14478..15299
                     /locus_tag="U4A13_001628"
     CDS             14478..15299
                     /locus_tag="U4A13_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1779928.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15350..17449
                     /locus_tag="U4A13_001629"
     CDS             15350..17449
                     /locus_tag="U4A13_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN1779929.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17446..18564
                     /locus_tag="U4A13_001630"
     CDS             17446..18564
                     /locus_tag="U4A13_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN1779930.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18603..19481
                     /locus_tag="U4A13_001631"
     CDS             18603..19481
                     /locus_tag="U4A13_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1779931.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19627..19824
                     /locus_tag="U4A13_001632"
     CDS             19627..19824
                     /locus_tag="U4A13_001632"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1779932.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         6357 a         3577 c         4415 g         6398 t
ORIGIN      
        1 tcggaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta
       61 taaacggcgg aagcagaaga aattccttcg gaaataagct gaaattcaca aaaatttgaa
      121 aagcaatttt cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct
      181 tttttgatca gcatgatttt attcagttat cttcaaaatg atttttccga tgtttttgtt
      241 tgcttccata tattcatgcg cttgtttgac tgcgctgaat tggaatgtac ggtcaatgat
      301 tggccgaatc ttgccatttt ctagaaaagg cccagctttt tgcataaaag tttgtgcgag
      361 ctctgcttta tactgatcac ttctaggtgt caataatgta ccaagcagtt ggacacgttt
      421 cccaatcaat acaccaaggt ctatctcagg cacgatggtc ccacctagca taccaatcaa
      481 gacccaacgt ccatcaacgg ctatagaagc taaattcttt tcccagtaag aagcgccgat
      541 gaaatccaaa ataacatcga ctccccgacc ttcagtgatt tttcgtacct cttcttgaaa
      601 gtcttgtttt ttatagttga tcaattcatc tgcaccaagt tctttacaaa aggcgagttt
      661 ttcttctgat cctgcagtaa cgatgatttt cgcgtgagaa agctgtctag ctaattggat
      721 cgctgctgtt cccacaccac ttgctcctgc atggatcaag acagtttcgc cttcttgtaa
      781 ttttccaagc cagtaaagtg tttgataagc agtcaaaaaa acttctgaga ttccagcagc
      841 agaaacatag tctagcgtat cagggagaag gattgcacga tcggcaggca tcacagcata
      901 ttcggcataa ccacctagat tgactaatcc gtatactttt gtcccaggag taaaagtgga
      961 gatatcactt ttattctcaa ctacctctcc agctacttcg actcccaaaa tagggtaggg
     1021 aggtttcaaa gagactgatt ctcttttcat aatatctgtt cgattgacag ctgctgtatg
     1081 gactttgatc agtaattcgc cttgttttgg tttaggaaca ggcacttcaa tttgttctaa
     1141 tacatctaca gaaccaggat gcttgattga aatggcacgc atggatctat tcctcctttg
     1201 cccattcttg gaatgctgta ttcaactctt tgctaccttc agaaagtact agagtcgtac
     1261 gagaaatatc cgtttcttcc actagagcgg caagtacagc agcaactgta gagcgagtga
     1321 tcgtttttga tgccttctca ttaggcttag ttaattgaat ggaagtaact tcttcatcgt
     1381 tagtcaatgt gactggttga agaatgacaa aatctaagtt agttcgtttc agccattcat
     1441 ctgcataatg tttagtaata taataatctg tcatggactc aggccatttt gctggttcat
     1501 ctgcataaac agcactgacc atcagatagc gagagacatg cgcattttca gctgcgatca
     1561 ctgttttgat tgcgccatcc aagtctactt gcagaagatt ctttccttga gagccggcag
     1621 caaaaatcaa tacatcgatg cctttgaatg cttcggtcat tttttccacg gaccaagtta
     1681 aatcaaaaga gacatacgaa acattctctt tttttactaa tgtttgggaa gcaacatctc
     1741 tcacccctgc aaaaatttca tgggaacttg gagctaaatc ttttatcagg tgtcggccaa
     1801 tttgtccgtt tgcacctaca acaaaaattt tcatttggat catcctttcg acgtcaaatc
     1861 tttccattca atagtaacac caaaagagaa aaaaatcaga gatcggctac gatgactatc
     1921 agttagtaag agaagggtgc ttgaagtatc ttaacaagac attgcatact gctgcagtcg
     1981 caagacctgc gacgattcct tcaggtatac cacttgtaaa aataaccgct agaatagctg
     2041 aataaacagc atttaaattt gttccgatga ctttggcata atcttgctgg aataaaaagt
     2101 agatcatatt catcactaaa atcgtattgg tcaatgaacc ggaaagtcca gctaagaata
     2161 aagaaacgga atctgctttt tgtttcatta gtttgcgcag acctttataa acgaaataag
     2221 gaaccacccc aatcaagatg cgaggaataa acgcgatcag caaggctttc cagcttccat
     2281 gttctgttcc gatgactggg ataaacggag aaaagacaaa agacaaaggt gtttggataa
     2341 ctgtgctacg gatcatgctg atcagtccaa atgtaccgcc aagaaaagca cctagtcttg
     2401 gtcctaaaac gatcgaagca atgattacag gaatatgcat ggttgttgca ttgattggac
     2461 cgatagggat aaaacctaaa ggcgtaacag ctaataaaat caaaatagct aaaaacatcg
     2521 cagttaatgt aaagtttttt gtatttttca taagtactcc ttttttctaa gcatttcttt
     2581 cattaagagt cagcatgacc tgttcgatga tcgtctgaat atcagctaag gcaccttttc
     2641 caaaatctcc acaagctaaa agagattctc ttggttcaat ttcctgatag ccgacagatc
     2701 tcaatgtttc cagattacgc tgagtgatcg ggttttgata catattcgtg ttcatggcag
     2761 gggcaatcaa ttttggtgtt cccactggaa gcgccattgc gactgttgaa agaagatcgt
     2821 ctgcaatgcc gtttgctagc ttgccgataa tgtttgctgt agcaggcgcg atgagaaaca
     2881 agtcagcttg ttttgctagt tcgatatgat tgattacctg cggatttgat tcttgcatga
     2941 cgtctgtatg gactgcgcgc ttcgacagag attgcaaggt taatggcgtg ataaaatgtg
     3001 tgctgctttt agacataatc acatctacct gggcacctaa tttgacgaat tgacttgtaa
     3061 gatcagcgct tttgtaagca gaaatacttc cagtaacgcc tagtaagatc tttttcattt
     3121 tgattcctcc agtctaattt tctcagtaat caatgcagca atctcagatt tactttgagc
     3181 ttcttccact gttccatctt tagataagag atagccgtgg tgtgtctcgc catggatttc
     3241 cgtcaaatca tttgcgaaaa taaaatcagt tttgttattt atcagactat tttttgcgac
     3301 tgccagaaga gattcctttg gtacatctac taatagtttg aaaccaatga tttttgcttt
     3361 tggctgccat tcacgtagat aagaaatgat ttttggtgtt ttcttcaaaa cgagcacaag
     3421 atgctcggtg ttagaagaga tctttgaaac agtggtatct gtctgttcat tttccgcaaa
     3481 ccaattgtcc agttcttctt gcgttgaaga agtgggtagg ttttttgcca gctgttcttc
     3541 tgaaaaacta aaagcaggag taaaatcact gactgccata ctgtggataa tcgcatcgta
     3601 gtgttctttt ttcgttagtt cttctaattg taaaaataag tccagtgcag attcgatcgt
     3661 ataaatcgtt atattggagt gtcttttcgg tttcagtgct tgtttcgtag tcacgtaatc
     3721 gattgtggta tccggattgg cagctaaata atcagctaat gcttgcccta atcgtcctgt
     3781 cgaatgattt gtaatagcac gtacttgatc gattttttca gtcgttcctc cagctgtgat
     3841 tagaatcttc atttatggtc acctacattt actagtgttc ttccttggtg tgttccagca
     3901 agtagtttag atgctgtttc aggcaattga tccagagtaa tctcttggac gaccagttca
     3961 tctgcaatct gcaaatccgc catctgttgc cataaagaaa gacgttgatc tattggtaca
     4021 ttgacagaat caattccaat caactggatg tttcgcagaa taaaaggcaa gacagtcgcg
     4081 tttagtttga ttcccccagc attgccacat aaagctactg caccgttata agagatcaat
     4141 ggaagaagac tgcttagctg ctcgccacct actgtgtcga tgacatagtc gatttgctgt
     4201 tttcctaaag cttttgttgt ctctggcaga aattcatcag gtcgtacgac ttgcaaagca
     4261 cctagacttt ttagccatgt gacagactct tcttttctgg aaaaggcaat gattgactgg
     4321 aatcctagtt tatgtaacag ggctatcgct gtactgccta caccgccaga agcaccagta
     4381 acgacaactt ttttatcgga ggtcatacct tgacggatta aagcgttcac acacaacata
     4441 gcagtaaacc ctgctgtacc aaggatcatc gcttgacgca gcgtcatatt ttttggtaga
     4501 ggaactaacc agtctcctgg tactttttga tattggctat aaccgcctgg atgggtgacg
     4561 ccaagcccaa aaccagtagc aagtacaaga tcttcttttg aaaaacgtgg attgttactt
     4621 tggacgacag ttccggataa atcaatccct ggagtcatcg gatatgttcg tatcacctga
     4681 ccagactcgc tcattgccaa tgcatcttta taattgacat cagaataggc aatttttacc
     4741 aagacttcat tttcagccaa ctcagtaaat ggcagatcac tgattgctgt atggaaaaca
     4801 ggttccttta ttacctgaaa agatcgaaaa gcgttcattc gtgagacact ccttttaaaa
     4861 ttttctttta tcataccata aagcctactc ttttattctt gttcttccgt aaatttagac
     4921 aaaaattagg tgaaaaataa agaatcaaac aactagaatt tattccaaaa gataaagaaa
     4981 tattcagcaa aaaaaatttt acaaagggtt gcctttttca ataaatttcg tatactaata
     5041 cgagtggtag taaacaggaa gtttactatt agaaaacttc gattcggagg aaattatgga
     5101 aattggtgag aagttaagaa atttgcgcat ccaaaagaat ttgacacaag aagaactagg
     5161 cgaacgcaca gatttgtcaa aagggtatat ttcccagttg gaacgagatt taagttcacc
     5221 atcaatggaa acattctttt ctattcttga agtcttaggt gtgactccag aagaattttt
     5281 tcatcaagaa gcagcgaatc tgcaagtggt ttattcaaaa gaagatcata cggtttattt
     5341 cgatgaagaa aacgggtatg aattggaatg gctcgtggct gattcgaatg aaaaagaaat
     5401 ggagcctgtc ttgctgactt ttgaaggtgc aggagaatat aagacgtttg aaccttcatt
     5461 atctgaaacg tttatttttg tattagaagg ggaattagag ttaagcttag gagagacggt
     5521 ctatgtggca aaagcaggac agtcgatcta ttatcaagcg acaaaacaac atcaattaag
     5581 gaatcatctc aaaaaacgaa caaaagtatt gattgttgca acgcaatcat atttgtaata
     5641 tagggggtaa aaacgtgggg aaaccaatca tttcatttga tcaagtagtg aaacgctatg
     5701 atgatgaaac agtattaaaa aaagtaagtt ttgagatcga acaaggaaaa ttttatacac
     5761 tgcttggtcc ttccggctgt ggtaaaacaa cgatcctacg tattatagct ggattcaccg
     5821 aagctacaga aggggacatc tattttgaag ggaaacggtt gaatgatttg ccagctaata
     5881 agcgacaagt aaatacagtc ttccaagatt atgcactttt tcctcatatg aatgtttttg
     5941 ataacgttgc ttttggattg aagatcaaga aaatgtccaa atcagatatc gaaaagaagg
     6001 taaaagatgc tttgcgtatg gtccagcttc ctggatacga aaaacgggag attagtgaaa
     6061 tgtctggtgg tcaaagacag cgtgtagcga ttgcacgagc aattgtaaat gaaccgaaag
     6121 tattgctatt agatgaaccg ttatcggcgc ttgatttgaa actacgtaca gatatgcagt
     6181 atgaactgcg ggatctgcaa caacgattag gaatcacgtt tatttttgtt actcatgacc
     6241 aagaagaagc gttggcaatg agtgatgaga tttttgtcat gaataaaggg aaaatcgtcc
     6301 aaagtggtac acccgtagat atttatgatg agccaatcaa tcatttcgtt gctgattttg
     6361 ttggggagag caatatcgtg gacggtgtaa tgatcgaaga taatctagtt gagtttgttg
     6421 gtaagcaatt tgaatgtgtc gatggcggga tgcgtctaaa tgaaccagtt gaagttgttt
     6481 tacgcccaga ggatttgacg atcacgacac cagataaagg aaaactagtc gtaacggtcg
     6541 atactcagct gtttcgtggt gtccattatg aaatcatctg ttatgatgaa cagcaaaatg
     6601 aatggatggt ccattcaacg aaaaaagcta aagaaggaag caaagtagga ttggcctttg
     6661 agccagaaga tatccatgtc atgcgtttca acgagtctga agaagaattc gatgcacgtc
     6721 ttgacagcta cgaagaatag gggggacaag aatgaaaaca atgcgcagga tctattccgt
     6781 cccttatatt ttgtggctcg gtttattcgt gatcgcgcct gtattaatga tcatttatca
     6841 atcttttttt gatatgaatg ggcaattcac gctgaataat tatctatctt attttacgtc
     6901 ggggacatcg ctttctatga cgatcaattc tgtatggtat gcatttttga tcacgttttt
     6961 cacgctgatc atcagttacc cgactgctta ttttttaaca aaactgaagc acaagcaatt
     7021 atggctgatg ctgattattt tgcctacatg ggtgaatctg ctgcttaaag cttacgcatt
     7081 tattgggatt ttcagtgtgc acggcagcgt gaatcatttc tttgatttta tcgggatcgg
     7141 gccgaaacag atattattca cagacttcag ttttctgttc gtggcaacgt atatcgagat
     7201 tccatttatg attatgccaa ttttcaatgc attggaagag atgaatcctt ctttgatcag
     7261 tgctagtaga gatttaggcg cgaatagctt tgagaccttc cgacgagtga tttttccgtt
     7321 atcattaaat ggtgtgaaaa gtggtgtcca agcagtattt attccttctc tttctttatt
     7381 catgctgact cgtctgattg gcggaaatcg ggtgatcact ttaggaacgg ctatcgaaca
     7441 gcatttcttg gttacacaaa actgggggat gggttctaca atcggtgtga ttttaatcat
     7501 tgctatgttc atcgtgatgc tcctgacagg cgagaagaaa aaaggaggtc gctcataaat
     7561 gaaaaaattt aaatggtcgt atctttattt agggatcgtc tttttattct tatatgcacc
     7621 gatcttctat ctgatttttt attccttcaa tgaaggagga acgatgaaca gctttacagg
     7681 atttacttgg gaaaattatg aagctgtttt tgaagataca cgtttgatca ttatcgtatt
     7741 gaatacgttc atgctggctt tcttatctgc attgatcgcc acgatcatcg ggacatttgg
     7801 ggctatggga atctattaca cgaaaaaacg acgtgcacgg acagcgttgt tgagcatgaa
     7861 taacatctta ttagtctctc ctgatgttat tatcggtgct agtttcttga ttttctttac
     7921 actactagga ttcggattag gttttcagag tgtattgctt tcccatattg ctttcagcat
     7981 tccaatcgtc gtcttgatgg tcttgccaaa attgcaggaa atgaacgatt caatggtcga
     8041 tgcggctaga gaccttggtg ctaataattt ccaagtagtc aaaaatatca ttttgcctta
     8101 tctttctcca ggaatcatag ctggatattt tatggcattt acctattctc ttgatgattt
     8161 tgcggttaca tttttcgtca caggaaatgg ctttacgacg ctctcagtag agatctattc
     8221 tcgtgcaaga caaggaatca gtttagaaat caatgcgcta agtgcgttag ttttcttgtt
     8281 ctccatgata ttggtcattg gatactactt tattagtaaa gaaaaccgtc caaaacggct
     8341 gaagaaaaac agaagagtac gcgagggggt ggcacggctg cgatgaaaaa attacaatct
     8401 ctgtttttag gtattctcat cattattgcg gtgctgctgc ttggtgttcg tcaacttgaa
     8461 aaaacaagtg gcatgagtgg agcaaaggta cttaccatct ataactgggg agattatatc
     8521 gatcctagtt tgatcacgaa atttgaaaaa gaatatggat acaaagtgaa ttatgaaaca
     8581 tttgattcta atgaagcgat gttcacaaaa atccagcaag gtgggacaaa ctacgacatc
     8641 gctataccaa gtgaatatat gatccaaaaa atgatcaaag aaaaactagt acttccatta
     8701 gatcattcga agatcaaagg attaaataat attgatccac gatttttaga cttggatttt
     8761 gatccagata atcagtattc gattccatat ttttggggaa ctttagggat cgtatataac
     8821 gataaattct ttgatgcaaa tcaaattaag cattgggatg atttgtggaa gccggaatta
     8881 aaagacagtt tgatgctgat tgacggtgca cgagaagtta tggggctttc tttgaatagt
     8941 ttaggctatt ctttgaatag taagaatatg gcacagttga cagaagcagc aaataaattg
     9001 aatcatttaa caccaaatgt caaagcaatc gtggcagatg agatcaaaat gtatatgatc
     9061 aatgaagaag cctccgtggc agtgacattt tctggcgaag ctgccgatat gatgtgggaa
     9121 aatgaacatt tacattatgt gatcccacca gaaggatcca atctttggtt tgataatatc
     9181 gttatgccaa aaaccgtgaa aaataaagaa ggtgcctatg atttcatcaa ctttatgcta
     9241 gaaccggaaa atgcggcaca aaatgcagag tatattggtt attctacacc aaatgaagaa
     9301 gcaaaagcgt tattgccaaa agacatttcg tcggacgaac aattctatcc atctgatgac
     9361 acgatctccc atcttgaagt ctacaaagat ctaggatcga aatacctagg catctataat
     9421 gacctattct tagaattcaa gatgtatagg agataatggc aaaactcagt aagaatgggg
     9481 acttatcaac gttctaaaga gctaaaaatc gtcctttttc tatctgagtt ttccaatctt
     9541 ctaccaaaag cggatgaaac gccgcttttt ttgtgttatt ttattttttc taccacgttt
     9601 ttctaccaga gaaaagtgga caaaaccatt gtaaaatagg gcccgagacg ggctagtaaa
     9661 tctaagattg aaaaagttac agaaggataa agtaattgga aactatgcaa atatacttaa
     9721 cccttctgta atctattaat aaaactaata tatgaatcca gttcaatatt tgtagctatg
     9781 aaaaaacata aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga
     9841 tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag
     9901 aatagacata ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa
     9961 aatgaaaaag agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat
    10021 gtcttctgtc actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag
    10081 ccattatttg acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt
    10141 tttgtggaag cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc
    10201 ctttcattct gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc
    10261 cgctgtcaaa tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg
    10321 aaaaagtttt ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa
    10381 agatgcacca tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc
    10441 ggcaacactg tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg
    10501 tttattggca acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa
    10561 agaaaatcaa gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga
    10621 aaatgtaaat tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga
    10681 aaattattta ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt
    10741 tgaagatcgc cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc
    10801 ggatgtttta caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca
    10861 tccctttagt gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt
    10921 gtatagtcga acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt
    10981 aaatatgctg gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa
    11041 tcaaaagata aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat
    11101 tactcagctg ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt
    11161 acagcatttg gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat
    11221 gattcagatg tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac
    11281 gagtggacct gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac
    11341 agacttaacc gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg
    11401 taatcagttc ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact
    11461 gcttattgtc gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt
    11521 gcttgctcac ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg
    11581 caattatgtt tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga
    11641 atcgacaagg aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga
    11701 tatgggctct ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact
    11761 tcttctgaat aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa
    11821 tgcgccattt aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata
    11881 ctttgaaggc ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat
    11941 ttcggacaaa ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt
    12001 cacgaaggac tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga
    12061 caagacaaaa ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat
    12121 taatgtttat gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc
    12181 tgaaatgaag ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt
    12241 ggaaggaatt gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga
    12301 aacagtcatt tctaaatatg aaaattacta tcatttcact ttaaatggcc gtgtaaaact
    12361 aaatctctat atgcatacgg cattgatgat ggaacgttta ttcttggctc ggacagaaaa
    12421 atctgaagaa ccgaattatg atttgaaacc agatgaagat gaattttatt atgtttctag
    12481 aagtattttt caatcaatgg agatgaaata taattttaaa gtcaatgctt atgagctttc
    12541 gctgctctat gaattattag gaccgtatat tcaaaaataa agtgtctaaa aaagtgtcta
    12601 atctgttttt tttaggttag acactttttt taatgtgaat taacgtgatt aaaccaatgt
    12661 ttttaaaagt tggcacagga attgcttata taaaagtgaa cagaaagaaa ggaattaacc
    12721 atatgaagaa aattctaata gcgactcacg gtcacttagc ttcaggcttt cttagcagta
    12781 ttcaattact gacggggaag gttcaagaga ttacggtgat taatgcttat gtggatgagt
    12841 gtgattttga acaggagttg aatgtatttg taaaccaggt gaacgagcag gatcagattt
    12901 ttgtttttac cgatttattt ggcggaagtg tcaatcagaa gattaccaaa acctttttgg
    12961 agaaagagat tgctgtaact attttgtctg gctttaatct gccaattctg ctcgaaatag
    13021 tattggcaac caaggaatta acaaaggagg atattcagga actggtaaat aaatgccaac
    13081 aggaattaaa gatgaccatt cttgatgagg tacatgtagt aaatgaagag gaagattttt
    13141 tttaggagga agaaaaatga ttaaagcagt gcgaattgat gaacgattaa ttcatggaca
    13201 agtagcgatg acttggacaa agagtctaaa tttaaccggg ttagtagtag caagcgatga
    13261 ggcggctacc aacgatattc aaaagatgac attgaagatg gctgcaccat cgaatgtcaa
    13321 agtcattatt aaaacagttg atgacgcgat tcatttgtta aaagatccgc gggcgaagga
    13381 catgcgcttg atggttttag tccctaatgt caaagatgcg gtaaaagttg cgaaagcttt
    13441 cccaacggaa atcgaattaa tgaacgtggg gaatgctggg aaaatgacac cgccatcgga
    13501 agataagaaa accttatcaa aggaagtgat gttaacgacg tcagaaattc aagacttaaa
    13561 agaattagtt gaactttatc cagataccgt ttttcagcct acaccggcga tggaaaaacg
    13621 ttcagctatc tctattttga gtgattttta gaaaaaggaa ggagaaaaat tatgtatgat
    13681 gcatttgtag tcgctttggc cgtttttatt ggaattgctg gacatgaaat gttcgggatg
    13741 tccatgctga gtcgtccgat tgttgttgct ccgttagcag gagcgttgat gggggatttg
    13801 cagacggggt tgattgtcgg ggcgtcacta gaagccatct ttatgggtgt cgtcaatatc
    13861 ggaatctcta gtacggcgga gccttctttg gctgctgggc tggctacagc gttcgcaatc
    13921 aaaatgggag gaaacgtgga tgcgattatt cccattgtct ttccactagc agtattagga
    13981 ttacagttaa tgaatatgat tttttctttt gtatgtggac cgatggctgc taaatttttc
    14041 aaatttgcaa aatctggtga tcaaaaagga atcatcaatc tgcacttctt tatgtggttt
    14101 gtccactatg cgctttatgc tcttattcca ttctttgccg ttttatttgg agcagatgtt
    14161 gtcaaaagcg tcttggataa tattccagag gtcatcatga acggattaac agtcgctgga
    14221 aatctgctgc cggcagttgg gatggcgatg ctgttacgga tgctttggag taaaaatatt
    14281 gctgtgtggt ttttctttgg ggccgttgtt atggcgtatc tcgagttacc attgattgca
    14341 attgccgcag ttggaacgat tattgcaatt gttatagccc aaagagatct acaactaaaa
    14401 aatatatctg ttcatgcagt tcaaagcgat gctgctgtaa atgcaacgct tgaccaagaa
    14461 gaggaggatt tctttcaatg aaattagctg atggaatttc taaagatgag aaaaaattgg
    14521 ttcggaagat gttttggcgc tcgatgacta tgtatgcttc ggtgaatccg gtgacgatgg
    14581 gcggcggtgg tttcgcctat tctatgatgc ccttcattaa tcatttctac aaaagtgaag
    14641 aagatcgagc tgcggcgctg gaacgtcata cacagtattt ttcgacgacc attccttgcg
    14701 cgtcctttgt catggggatt gcggcatcga tggagaagga aaattctgaa aaagctggtt
    14761 ttgacgtgga aagtatcaac gcgatcaaaa cgagtttgat gggaccattg gctggaattg
    14821 gcgattcatt gttctgggga gtttggcgtg tcgttaccgc gggactagcg atcaacttag
    14881 ccaatgccgg aaatattttg gcaccaatta tctttttagt tatgttcaat atacctaatt
    14941 acttgatgcg atactatggc ggcgttttag gctattcatt aggctctaaa tatattgaaa
    15001 agctgtatgc gaacgggtta attgaaattc tgacaaaagc tgctagtatc ttgggtctga
    15061 tcatggtagg tgcaatgacg atcagtatgg tcaaattcaa taccgttttg agcttttcaa
    15121 tgaagggcga agaactaatg aatgtccaag aaatcctcga tagtattttt aaaggaattg
    15181 taccgttatt ggtaacattg agctgtttca aattactaga taaaaaagtc ggcgtcatca
    15241 aaattatttt tggaatggtc gtcttgggaa ttttattgtc attacttgga atcgcatagt
    15301 aggaagctgc agctactagg ctgcagtttt tttagcaagg agggaaaaga tgactattaa
    15361 tgaagtaatt tattcaccca aacacccatt ttttaatgct gagttaaacg acggaaatgt
    15421 gactacttca aacgaataca gctactatcg gaatattcct gatgtaaagg gattgacctt
    15481 cgatgtaagt aatcaaaaga cattagcaaa ccttgatgtt tgcgggacga ttcaaacgtt
    15541 gaccttttat cgagaaaatt tactaactga ggaaaaacca ggtgtttggg tcaataaaca
    15601 gctctctcaa accaattcgt tatccttaaa ggttgaagta aacgggattg tctaccattt
    15661 gtcagaatcg gatcatcgta ttgaagtcga tgtgattcaa gactgtttgc ctagatatat
    15721 tcatcattat tcgacgttta aggtcattgt agtatccttt gctcccatac tggctaataa
    15781 gcgtttaagt atgcttgttc aacaggttta cattgtgaat gaaacacacg aaagccttca
    15841 agtttcagtg aaagatgtgc cttcgtatca agagaagtat tcggatcagc aaaatgtgtt
    15901 gatttctcaa aaaggaaata aggacacgat cgcacaacag gaagtggcaa gcttcgccat
    15961 tgcttttgtg gacccaaatg cttttgaaga agtaatgact tttcaggaat ctgatatcga
    16021 aaaatggctg aaggaaacat tgaactattt tgctcagcta tttgggcagc tgtcaatgcc
    16081 aaacgataga gttgtccatc tctatcgtcg tgcactttat caaagctttt ctagtttcgg
    16141 tatgaatcga cagggacagg tcgtcggcag caattggggc agctatcctg ctactaatcg
    16201 aatttggaat aaggatatgt attacgcatc attgccattc accatgtttg aaccggagtt
    16261 atgtcaaaaa acggttctat ggtttgatca atatggcgtt aagtttcctg gttcaaaatt
    16321 tacaggaggt atcaatcatt ctctgagtaa ttcgttatcc agtgcgttac tggctagtct
    16381 ttattttgaa catactgggg atacaaaatt ctttgagcgg tgtccgcagg ttctactgaa
    16441 tgcttcaaga attattgagg acatattagc tcaacgtcgg tcaggggaac caatgctttt
    16501 tccatctgtt tggttatcag acgcctttgc acttggaaag tatcataccg gcagcaatct
    16561 ttgtctttgg aaggcctgta ttggacttgc agaaattttt gaagccctag aacaactgag
    16621 tcttagcaag cgatacaaaa acattgcctg taaactaaag gaatcaattt gtaaacaaat
    16681 gactatcgat ggtccttttg gtgagcaatt tttggaggga atcggtgatg aggagaaaac
    16741 aacctattca gtgaagcact atcaaaaacc gattcttgag caagggttaa tctttttaag
    16801 tgatgtgatt gtggatggaa aaattaattt attgatgcat gatggagaag aatcagacac
    16861 tacgttgatg cctttttatc aatttttaga taataaagat catttatatc agaacaccat
    16921 ggccttttct gctagttctt tcaatccaac ctatagcgag gaaatcaaag gcatcacttg
    16981 gggattagaa tccggcgcca cttttcctgg ctttatcact gttttaatga gcgagctgca
    17041 caaccctcaa gctttcgtaa cccgattaga agaactaatt tgtttggcag atttagacgg
    17101 ttcgtggtgg tggtggccgt atcgcttaga ggctcaacaa ggagaagtcg tgcgagactt
    17161 tggctgcggc aaatgcggct gggcatcggg tctttttgtc agcctaatga tcagtcaata
    17221 tttcggattg aaattcaaaa aaggagtatt gcaaattgat cctgttgata atttaacgtt
    17281 ttcttggaag aatttgaagt tcggtcaagc cttcatttcg attgaatgta cgcaatccga
    17341 gttgagtatt tcaaatttgt cagaaaagcc attgaaaata agtgatacga aaaaaattct
    17401 tcacgtagaa aagggaaaaa cgattcacat tcaaaggaga aaaagatgag tatactaaaa
    17461 aatattaccc aatttaaaaa aatccagagt cgagcaatca ctcccgaaaa tccaacaggc
    17521 gagaaaggta aagctgcgat gttgccaagc gagctaggac caagccgcaa aggtcgtggc
    17581 gcaattccgc tgacttctgg tgaagaaact gtgattgcgg atattactgg aacgggaata
    17641 attcgtcata tgtggatgac gattcgtgat cgaacagaat ttggcagttt tgttttacgt
    17701 gatttaatct taagaattta ttgggatggg tcggatagcc ctgctgttga aagtccttta
    17761 ggggatttct tttgtaatgg gtttggagag cgttgcgagg tgaactcgtt gccgatagtg
    17821 gttaatccta cagggggctt taattcctat ttcgaaatgc ctttccaaac atccgctaaa
    17881 attacgatcg agaatttaca tccgaaggat atgtcctcat ttttttatac gataaattac
    17941 gctttggtgg acgaattgga agaaaatagc ctatattttc acgcaaattg gcatcgggaa
    18001 cgcattacca aattacagga ggattataag ttaattgatc gattaaaggg tcaaggctat
    18061 tacgtgggta cttaccttgc gcttacctgc ctagaacgtt attggtgggg cgaaggcgag
    18121 tttaaattct acattgatgg ggatagtgag tatccgaccg taacctcaac aggttcagaa
    18181 gattattttg gcggggcgtg ggcctttcat gagtacgatg agttcggacg tccacatgcg
    18241 aaaaactatt caacagcctt tttagggtat ccttaccaat caaatagaga tgccacaaga
    18301 gatcgttttg aaacgggaaa attgaatgcc gttcatgcct ttggtgacga cgcaatgcca
    18361 cgacatggtc tttatcgttg gcatcttcaa gaccccatct gttttaccga agatttatcg
    18421 gtcactttac aacaaatcgg caacgatgat gttcgcttat ttgagcggca agacgatgtt
    18481 gctagtgtcg cttactggta ttcaacagaa gcttcaggaa atactaaacg tcatttcact
    18541 gatcgagaac gcttgccgcg ttaatgaagt gtttttcaca tattcgaagg gaggaaccta
    18601 tgatgatata tggggcaatt gaagctggag ggactaaatt tgtctgtgca gtcggtactg
    18661 aggaattaac gattttggaa agagtcagtt ttcccacaac aactccagtt gaaacaatga
    18721 agcaggtcat tgattttttt aaacctttta aggaacaatt ggcaggcatc ggggtcggct
    18781 gctttggtcc aattgatatt caatgcaaat caacgagttt cggtcatatc acatcaacac
    18841 caaaacttgc ttggcaaaat ttcaattttg taggaacgct aaagcaatat ttcaatattc
    18901 cgattgaatg gacaacagat gtcaatgcgg cttgctatgg tgaatatgtt gctggttctg
    18961 gcaaagggtt agcaggtgtt gcatacttca ctgtgggaac aggtatcgga ggtggtgcct
    19021 tgttcaatgg gactttcgtg gaagggttca gtcatccgga aatggggcat atgttgatca
    19081 agcgacatcc aaaggacaat ttttcaggaa actgtccctt ccaccaagat tgtttagagg
    19141 ggatggcagc aggaccagcg attgaaaaac ggttgggtgt aaaaggccag aatttattgg
    19201 ctgacgattc gttttggcag attgaagcct tttaccttgc ccaatgtgca tacaatacaa
    19261 cactgatgtt ttcgccagat agaattattt ttggcggtgg tgtaatgaaa caagaacaca
    19321 tgaagaaaaa agttcaggat aaatttgttg agcttataaa tggatatgtt gaaataccac
    19381 caattgatag ctacattatt acgcccgaat taggtgataa tgccggaata atcggcggat
    19441 tagcattggc cagaaaagcg gtaagaaata agcaaccata attaatgaat gtttcattga
    19501 tactcatgtc aaaagtattt aaccgagtaa tcaaatgatc aaatgaagtt ggataaagcg
    19561 aaaatgaatt caaataaaaa ggctctaagt caaatcgtgt tgttttcatt aaatcactga
    19621 aaggaaatgc ttctgcgttt ccttttttct ttttgttgtg cgctgtatac cttgaggata
    19681 ttcactggtg gcgtacactt aaatagtaat atattgtgtt actttatgtc tgtggtaata
    19741 gttatagttg cttaccaatg tatgttcatt tttcgaaatg ttgacttcat aatccttggc
    19801 ttaatgtttg aatttttttg gtgatagatt ttttcagccg tataaaataa atcgtaattc
    19861 tattatagag agttgtgatt ataaaaagat agcaagaaaa aaaaagaaag tttctataca
    19921 ttttaaatat tttattccta tttctattgc taaaaaagta gaaattctat atttaccttt
    19981 aactctcgct tctcttgcaa atgtagttag cattttaatc gataataaac tcaggtagtt
    20041 atattacctt gaaatgattg ataattttgt cacttctatt ttttttgcaa taaatttttt
    20101 attttagtga aaaataagaa actatttctt tgaaggcgat aagttagaaa aatattattg
    20161 tagtcttttc tttattagga atcatcactg ttgtatctta ttaccttttc tgtttttctt
    20221 attagagaaa aataatttga gaggtagata gtttttattg gaggaagtaa ttatgtatat
    20281 ttttgaaaca aaaatttttc attgcaataa ttgcaacaaa gaaatttcat cgggggaaga
    20341 ggtttggaca aaaatggaaa tttccgccaa aagtaactgc tcctcaactg aaatcaatta
    20401 aagaactaga atttgagaat gcactggtaa tttgtatgac atgtactgat gaagtatttc
    20461 gaaaacattt ttaaaggttg agatgtatat agatttaacc aatcatttat ctctctcgga
    20521 aaaggaatat taactaacca gcgggttatc aaaaataggt aggaaaactg tacttaatgg
    20581 cttatttcgc gctgattatg agaatatcag tctgtttgat taatttttag ggttcaaaat
    20641 acagagacaa actccaggtc gggtttcgcc acaagcgaat tacccgatct ggagttttgt
    20701 ctattgcagg aaggcgtagc cggactgcag gaataaagcc cccggtt
//
LOCUS       DASJPC010000046        20595 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJPC010000046 DASJPC010000000
VERSION     DASJPC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.046"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            99..293
                     /locus_tag="U4A13_001633"
     CDS             99..293
                     /locus_tag="U4A13_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779933.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="U4A13_001634"
     CDS             562..861
                     /locus_tag="U4A13_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779934.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="U4A13_001635"
     CDS             complement(958..1392)
                     /locus_tag="U4A13_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1779935.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="U4A13_001636"
     CDS             complement(1624..2334)
                     /locus_tag="U4A13_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN1779936.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="U4A13_001637"
     CDS             complement(2324..3184)
                     /locus_tag="U4A13_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN1779937.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="U4A13_001638"
     CDS             complement(3189..3827)
                     /locus_tag="U4A13_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN1779938.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="U4A13_001639"
     CDS             complement(3842..4141)
                     /locus_tag="U4A13_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1779939.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="U4A13_001640"
     CDS             complement(4184..5668)
                     /locus_tag="U4A13_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN1779940.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="U4A13_001641"
     CDS             complement(5688..6149)
                     /locus_tag="U4A13_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1779941.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="U4A13_001642"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="U4A13_001642"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN1779942.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="U4A13_001643"
     CDS             7452..8222
                     /locus_tag="U4A13_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1779943.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="U4A13_001644"
     CDS             complement(8495..9322)
                     /locus_tag="U4A13_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779944.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="U4A13_001645"
     CDS             9890..11125
                     /locus_tag="U4A13_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1779945.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="U4A13_001646"
     CDS             complement(12124..12384)
                     /locus_tag="U4A13_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN1779946.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="U4A13_001647"
     CDS             complement(12411..13712)
                     /locus_tag="U4A13_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1779947.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="U4A13_001648"
     CDS             complement(14004..14462)
                     /locus_tag="U4A13_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1779948.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="U4A13_001649"
     CDS             complement(14475..15806)
                     /locus_tag="U4A13_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN1779949.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="U4A13_001650"
     CDS             complement(15817..17259)
                     /locus_tag="U4A13_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1779950.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="U4A13_001651"
     CDS             complement(17246..18010)
                     /locus_tag="U4A13_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN1779951.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="U4A13_001652"
     CDS             complement(18114..18989)
                     /locus_tag="U4A13_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1779952.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="U4A13_001653"
     tRNA            complement(19244..19328)
                     /locus_tag="U4A13_001653"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="U4A13_001654"
     tRNA            complement(19346..19417)
                     /locus_tag="U4A13_001654"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="U4A13_001655"
     tRNA            complement(19427..19499)
                     /locus_tag="U4A13_001655"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="U4A13_001656"
     tRNA            complement(19561..19645)
                     /locus_tag="U4A13_001656"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="U4A13_001657"
     tRNA            complement(19666..19736)
                     /locus_tag="U4A13_001657"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="U4A13_001658"
     tRNA            complement(19782..19853)
                     /locus_tag="U4A13_001658"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="U4A13_001659"
     tRNA            complement(19865..19937)
                     /locus_tag="U4A13_001659"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="U4A13_001660"
     tRNA            complement(19940..20013)
                     /locus_tag="U4A13_001660"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="U4A13_001661"
     tRNA            complement(20020..20100)
                     /locus_tag="U4A13_001661"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="U4A13_001662"
     tRNA            complement(20102..20177)
                     /locus_tag="U4A13_001662"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="U4A13_001663"
     tRNA            complement(20213..20285)
                     /locus_tag="U4A13_001663"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="U4A13_001664"
     tRNA            complement(20295..20367)
                     /locus_tag="U4A13_001664"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="U4A13_001665"
     tRNA            complement(20383..20454)
                     /locus_tag="U4A13_001665"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="U4A13_001666"
     tRNA            complement(20469..20558)
                     /locus_tag="U4A13_001666"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
BASE COUNT         6501 a         4312 c         3269 g         6513 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgag
//
LOCUS       DASJPC010000047        20486 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJPC010000047 DASJPC010000000
VERSION     DASJPC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.047"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="U4A13_001667"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="U4A13_001667"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN1779953.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="U4A13_001668"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="U4A13_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN1779954.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="U4A13_001669"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="U4A13_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN1779955.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="U4A13_001670"
     CDS             2857..3732
                     /locus_tag="U4A13_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1779956.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="U4A13_001671"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="U4A13_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN1779957.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="U4A13_001672"
     CDS             4629..5063
                     /locus_tag="U4A13_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN1779958.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="U4A13_001673"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="U4A13_001673"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN1779959.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="U4A13_001674"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="U4A13_001674"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN1779960.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="U4A13_001675"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="U4A13_001675"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN1779961.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="U4A13_001676"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="U4A13_001676"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN1779962.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="U4A13_001677"
     CDS             13334..14260
                     /locus_tag="U4A13_001677"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN1779963.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="U4A13_001678"
     CDS             14442..15335
                     /locus_tag="U4A13_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN1779964.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="U4A13_001679"
     CDS             15469..15594
                     /locus_tag="U4A13_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1779965.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="U4A13_001680"
     CDS             complement(15728..16360)
                     /locus_tag="U4A13_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779966.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="U4A13_001681"
     CDS             complement(16508..17050)
                     /locus_tag="U4A13_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN1779967.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="U4A13_001682"
     CDS             17185..17574
                     /locus_tag="U4A13_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779968.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="U4A13_001683"
     CDS             17578..18342
                     /locus_tag="U4A13_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1779969.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="U4A13_001684"
     CDS             18471..19418
                     /locus_tag="U4A13_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1779970.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="U4A13_001685"
     CDS             19548..20288
                     /locus_tag="U4A13_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779971.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5496 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagttt
//
LOCUS       DASJPC010000048        20188 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJPC010000048 DASJPC010000000
VERSION     DASJPC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.048"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..152
                     /locus_tag="U4A13_001686"
     CDS             <1..152
                     /locus_tag="U4A13_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1779972.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="U4A13_001687"
     CDS             complement(230..763)
                     /locus_tag="U4A13_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN1779973.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="U4A13_001688"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="U4A13_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN1779974.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="U4A13_001689"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="U4A13_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN1779975.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="U4A13_001690"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="U4A13_001690"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN1779976.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="U4A13_001691"
     CDS             complement(4488..5066)
                     /locus_tag="U4A13_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN1779977.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="U4A13_001692"
     CDS             5388..5858
                     /locus_tag="U4A13_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN1779978.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="U4A13_001693"
     CDS             complement(5966..7192)
                     /locus_tag="U4A13_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1779979.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="U4A13_001694"
     CDS             complement(7946..8146)
                     /locus_tag="U4A13_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1779980.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="U4A13_001695"
     CDS             complement(8280..8888)
                     /locus_tag="U4A13_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN1779981.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="U4A13_001696"
     CDS             complement(8973..9443)
                     /locus_tag="U4A13_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN1779982.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="U4A13_001697"
     CDS             complement(9491..10405)
                     /locus_tag="U4A13_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN1779983.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="U4A13_001698"
     CDS             10551..11354
                     /locus_tag="U4A13_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN1779984.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="U4A13_001699"
     CDS             11532..12479
                     /locus_tag="U4A13_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN1779985.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="U4A13_001700"
     CDS             complement(12547..12840)
                     /locus_tag="U4A13_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN1779986.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="U4A13_001701"
     CDS             complement(12868..13533)
                     /locus_tag="U4A13_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN1779987.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="U4A13_001702"
     CDS             complement(13673..14305)
                     /locus_tag="U4A13_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1779988.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="U4A13_001703"
     CDS             complement(14312..15379)
                     /locus_tag="U4A13_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1779989.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="U4A13_001704"
     CDS             complement(15376..16107)
                     /locus_tag="U4A13_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN1779990.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="U4A13_001705"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="U4A13_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN1779991.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="U4A13_001706"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="U4A13_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEN1779992.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKCRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="U4A13_001707"
     CDS             18270..19439
                     /locus_tag="U4A13_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN1779993.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..>20188)
                     /locus_tag="U4A13_001708"
     CDS             complement(19706..>20188)
                     /locus_tag="U4A13_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1779994.1"
                     /translation="NYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQ
                     LLLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNAT
                     VLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE
                     "
BASE COUNT         6109 a         4026 c         3211 g         6842 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggcattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataatt
//
LOCUS       DASJPC010000049        19457 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJPC010000049 DASJPC010000000
VERSION     DASJPC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.049"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            133..3660
                     /locus_tag="U4A13_001709"
     CDS             133..3660
                     /locus_tag="U4A13_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN1779995.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="U4A13_001710"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="U4A13_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN1779996.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="U4A13_001711"
     CDS             7448..7789
                     /locus_tag="U4A13_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1779997.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="U4A13_001712"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="U4A13_001712"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN1779998.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="U4A13_001713"
     CDS             9146..11566
                     /locus_tag="U4A13_001713"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN1779999.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4A13_001714"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="U4A13_001714"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN1780000.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="U4A13_001715"
     CDS             13000..13737
                     /locus_tag="U4A13_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1780001.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="U4A13_001716"
     CDS             13751..14566
                     /locus_tag="U4A13_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN1780002.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="U4A13_001717"
     CDS             14579..15226
                     /locus_tag="U4A13_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1780003.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="U4A13_001718"
     CDS             15242..15898
                     /locus_tag="U4A13_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1780004.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="U4A13_001719"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="U4A13_001719"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN1780005.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="U4A13_001720"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="U4A13_001720"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN1780006.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="U4A13_001721"
     CDS             18240..18758
                     /locus_tag="U4A13_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1780007.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="U4A13_001722"
     CDS             18865..19365
                     /locus_tag="U4A13_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1780008.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DASJPC010000050        19301 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJPC010000050 DASJPC010000000
VERSION     DASJPC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.050"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="U4A13_001723"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="U4A13_001723"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN1780009.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="U4A13_001724"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="U4A13_001724"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN1780010.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="U4A13_001725"
     CDS             complement(2303..2965)
                     /locus_tag="U4A13_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1780011.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="U4A13_001726"
     CDS             3149..4795
                     /locus_tag="U4A13_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1780012.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="U4A13_001727"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="U4A13_001727"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1780013.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7529)
                     /locus_tag="U4A13_001728"
                     /pseudo
     CDS             complement(5976..7529)
                     /locus_tag="U4A13_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7677..8818
                     /locus_tag="U4A13_001729"
                     /pseudo
     CDS             7677..8818
                     /locus_tag="U4A13_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(8944..10023)
                     /locus_tag="U4A13_001730"
     CDS             complement(8944..10023)
                     /locus_tag="U4A13_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1780014.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10126..10566)
                     /locus_tag="U4A13_001731"
     CDS             complement(10126..10566)
                     /locus_tag="U4A13_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1780015.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(10623..11033)
                     /locus_tag="U4A13_001732"
     CDS             complement(10623..11033)
                     /locus_tag="U4A13_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1780016.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(11213..11647)
                     /locus_tag="U4A13_001733"
     CDS             complement(11213..11647)
                     /locus_tag="U4A13_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1780017.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(11747..12595)
                     /locus_tag="U4A13_001734"
     CDS             complement(11747..12595)
                     /locus_tag="U4A13_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1780018.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            12984..14750
                     /locus_tag="U4A13_001735"
     CDS             12984..14750
                     /locus_tag="U4A13_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN1780019.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            14852..16111
                     /locus_tag="U4A13_001736"
     CDS             14852..16111
                     /locus_tag="U4A13_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1780020.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            16258..17559
                     /locus_tag="U4A13_001737"
     CDS             16258..17559
                     /locus_tag="U4A13_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1780021.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            17562..18413
                     /locus_tag="U4A13_001738"
     CDS             17562..18413
                     /locus_tag="U4A13_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1780022.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            18426..19241
                     /locus_tag="U4A13_001739"
     CDS             18426..19241
                     /locus_tag="U4A13_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN1780023.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
BASE COUNT         6364 a         3509 c         3579 g         5849 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
     7441 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagata
     7501 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
     7561 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
     7621 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
     7681 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
     7741 agacagtcca cacagaggag acagaaacag ccaaatggac agaaaccaag tgggccaacg
     7801 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
     7861 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcgggagac ttagcaccta
     7921 tactacaatc agctgttaca tttgagaatg ttgatcccac tttgtcgatt ggctatgacg
     7981 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
     8041 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
     8101 atcatttaac gccgtatagt cagcggcaga caattgagca agattcagcc atcgactcag
     8161 gaggagatct tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac
     8221 cgcctacaga aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg
     8281 atacagaaac tcaaccagag ccagacaata aatcaaaacc tgaaataacc cctggagaaa
     8341 aaccagatgt aactccagaa ccagatacgg attctggaaa ccaaacagta ccggaaacaa
     8401 atccagacac agataatgaa actgaaaatc cagaaaaacc agaggtagat ccagaagaaa
     8461 aaccagatgt gactccagaa ccagatacgg acgcaagaga ccaaggaata ccagaaaaaa
     8521 taaacaaaaa aacaattcaa gaggatggaa aaaaagagag taaaaaatca aatctagcaa
     8581 ttctaaaaat aaatgaagag cagctgaata aaaaatcacg aatatttgat agcgcccaat
     8641 cagctgaaac cttaaaaagt agcaaggata caacctttgc tgtcctgaaa cgaaaaataa
     8701 acagctacca aaatccggag agtcacaaaa caaagttata ctgtggagcg gaatcattct
     8761 attaagtata gcaacaatgc tttctgcaaa aagatttaaa caaaacagat ctctctaaca
     8821 tttgaataat gtctgtgaac gcatttttca aataaaaaga aatatccaga aaatgacccc
     8881 aaaataccag attgaaatct gatatttggg gtcgttcata ctttatcttt cttcatattt
     8941 attttaaggg atatctgttt cttttggaag atggttcggg atctttttca ctttcaatac
     9001 atctaaaaag aaggtaaaga tcggaatgcc gacaatcaag ccccaaacgc caaagaaccg
     9061 ttcgctaaca agtaagacca caaatgtata aaaaatcggc agttctgttt tgctggacat
     9121 caacttcgga ttcagcacat aagactcaat tagatggaca atcaaaatcg taatcaggat
     9181 ataaacaaca tctcggatgc ctccttggga ataagctatg aaagatagag gaatacaaga
     9241 aatgataaca cctgctactg ggatcagact taaaatgaaa atcatgatcg ctaaactcag
     9301 cagctgatga aagccaaata cagccaaggc gattgtagtg agcaacgtat ttaaaagagc
     9361 gacaacaaat tgagcttcta ataccaaacc aaatgtattg acaaacttat cggcaaaata
     9421 gtaaatatct tgaaagaacc aagaaaattc actttttaaa aacaatcttg agaatacaat
     9481 tgtttttttc ttttcaatca taaaaaagaa acttaagatg aatgacatag caaacgccac
     9541 tgccattttt cctatatcct gtaaatagcc aagcgcaatc gaagcgccat tttgaagctg
     9601 ttcaaaaaaa tcgtttcgct caatataagt atggatatat tgtaaaacag gatctgcatc
     9661 ttttggctga tgctcgtaaa aattaataac ggactggatc atgtcaaatg tttgatgaat
     9721 aagcatcggg atatactttg tgaatgccag atagataagg taaacgatca gtgtataaat
     9781 catgatagct aaaacacctg tagggattcg gacaaatcgc tgaatgattt gaacaagatg
     9841 aatcatcaaa taagtaaaga taaatgtgag caaaatggtt gtaatcatac ttctaactaa
     9901 gaaaagaaca aaaacgacaa ataataaaac agtgagacgg cgtatttttt cattcgccaa
     9961 aaaacgctga taaacggtca aatcgacaac ctccaaataa caaaaaataa acacgttatt
    10021 cattatatga tggaatatga aaataagaaa gagcaaaaag aaaaaccgct aaatgacgtt
    10081 atcttatgag ttggttttcc tcataaaatc gttcatctag cagtttcagc cctttttctg
    10141 gtcttgatga aaattatcta gcatttttcg ggtcagatgt tgcagtgtat ccaattcttc
    10201 ctgttcaagt ggaagataag acttgatctt ttgtggaata ccttctgctt gtttttgcaa
    10261 cgcagcacct tgagcagtca gcgtaacatt tacgattctt tcatcacccg tttcacgctg
    10321 acgattaata taattttttt ctgctaactt ttttagtaag ggagtcagcg taccagaatc
    10381 cagacacaaa tcatttccta gctctttcac tgaagtcttt ttcttttccc ataaaactaa
    10441 catcacaaga tactgggtat aggtaagatt cagcggcttg aggaggggag tgtagtgtct
    10501 aatgatttct ttagaggcgg catataaagg aaaacatact tgattaacta aatatagatc
    10561 ttccattttt ccacctccgc tgttttacta tagtgtatca tagcggattt acacaaaaaa
    10621 gtctattcac taactgcttt cagagtaact tctatatttc cagctgtagc ttttgaatac
    10681 ggacacactt tatgcgctgc ctctaataat tcttgcgttt cttccagaga aagaccttcg
    10741 atagatcctt caatctctac acctaagaca aagctcgttt cactttctga ataaagggaa
    10801 acttgtgcac tgacagtaga agccccagag atattttttt gctgtttgat caattctaat
    10861 gcactgttaa aacatgcact gaaaccagct gcaaacaatt gttcagggtt ggttgcattt
    10921 tcgacgcgtt taccaggagc tgcaatattc aattcgaatg tacgatcggg attatgcact
    10981 tctccagagc gtccgcctgt gttgatcatt ttcgtagaat acattttttt cataaaatta
    11041 ccacctttaa ttatatttta tacaattgaa ttttacacaa tataattgtg gaaagcaaag
    11101 aaatggattg gcataagata aataagagaa agcacatgaa acagacgcgc tttctcgaat
    11161 agcaaacata tacaaaaaat atcagagaca aaaaataatc tcatcataaa cattacatat
    11221 atttctcaaa aagttgtttt ctttcttcaa aaattacttc acgatggacc tgatgaagca
    11281 tccataaata accaaaagga tctctgaaca ttgagttagt cacaccaaag tccatcattt
    11341 ctgtcatttc ttgaatctct acacagccat ttcgtaatgc tttattatgt gtgtcctcga
    11401 tatcagagac gatgatattg aaccagatcg tatttggatg ttcagcagta ggagcagtca
    11461 aaccaagaga cgtgttttca tccagcatat ggaatctcgt accatacaag gtaaaaatga
    11521 cttcattatt gcctctaggg taatctgttg cctcaatcag ttgtatttca tcaaagattg
    11581 tttgatacag ttctagtgct ttcaaactat catttaatac aaaatcgatt tccgttcctt
    11641 tatgcataaa gatctcctct ccttttcagt cgaacagtta ctagattctt ttattatacc
    11701 agctaaagaa aacgcattca aaaaaatagc ggatttactt accaactcat tcgattggat
    11761 gataagtaaa tccgcgtccc attacttctg ctacgctgct gacaatgaca aaggcacttg
    11821 gatcgatttc ttctatcaat ggtttcattc gggaaaattc atgatcagtc acaacgatca
    11881 ataaaacctc ttttgacttt cgattatacc cgccttttgc ctctagcagg gtaaggccac
    11941 gtccaatctc agcacttaag cgaaggcgga tgtcttccaa ataatgttca ctcatgacca
    12001 taacagattt ctttcgattg atacctgttt cgatataagt catgacaatt gaagtgattg
    12061 caatagataa aatagcataa agaaactctt ctattccaaa aataaacaga ttaagagaaa
    12121 caacgaatgc gtctgtaaaa aataagccaa tcgaagtatt caagtggaaa tattttttaa
    12181 aaatgagggg agggatcgtc gtaccgccgc tagaagagtt gtttttatat aaaatcgcaa
    12241 ctcctaaagc aaaaatcgca cttccaaaaa tgacagacaa caaccgatct gaagtaatca
    12301 ttgtttctgg aactattgca atgattaaag gaaaaaccaa gcttccgaaa agtactttaa
    12361 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
    12421 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
    12481 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
    12541 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
    12601 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
    12661 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
    12721 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
    12781 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
    12841 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
    12901 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
    12961 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
    13021 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
    13081 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
    13141 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
    13201 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
    13261 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
    13321 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
    13381 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
    13441 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
    13501 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
    13561 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
    13621 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
    13681 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
    13741 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
    13801 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
    13861 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
    13921 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
    13981 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
    14041 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
    14101 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
    14161 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
    14221 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
    14281 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
    14341 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
    14401 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
    14461 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
    14521 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
    14581 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
    14641 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
    14701 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
    14761 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
    14821 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
    14881 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
    14941 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
    15001 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
    15061 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
    15121 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
    15181 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
    15241 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
    15301 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
    15361 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
    15421 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
    15481 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
    15541 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
    15601 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
    15661 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
    15721 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
    15781 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
    15841 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
    15901 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
    15961 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
    16021 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
    16081 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
    16141 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
    16201 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
    16261 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
    16321 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
    16381 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
    16441 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
    16501 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
    16561 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
    16621 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
    16681 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
    16741 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
    16801 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
    16861 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
    16921 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
    16981 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
    17041 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
    17101 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
    17161 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
    17221 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
    17281 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
    17341 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
    17401 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
    17461 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
    17521 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    17581 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    17641 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    17701 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    17761 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    17821 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    17881 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    17941 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    18001 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    18061 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    18121 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    18181 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    18241 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    18301 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    18361 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    18421 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    18481 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    18541 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    18601 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    18661 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    18721 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    18781 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    18841 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    18901 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    18961 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    19021 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    19081 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    19141 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    19201 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    19261 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt t
//
LOCUS       DASJPC010000051        19126 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJPC010000051 DASJPC010000000
VERSION     DASJPC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.051"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..703)
                     /locus_tag="U4A13_001740"
     CDS             complement(<1..703)
                     /locus_tag="U4A13_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1780024.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="U4A13_001741"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="U4A13_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="U4A13_001742"
     CDS             1758..2837
                     /locus_tag="U4A13_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN1780025.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="U4A13_001743"
     CDS             2869..3462
                     /locus_tag="U4A13_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1780026.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="U4A13_001744"
     CDS             complement(3572..3799)
                     /locus_tag="U4A13_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1780027.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="U4A13_001745"
     CDS             complement(3902..6490)
                     /locus_tag="U4A13_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN1780028.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="U4A13_001746"
     CDS             complement(6510..7169)
                     /locus_tag="U4A13_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1780029.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="U4A13_001747"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="U4A13_001747"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN1780030.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="U4A13_001748"
     CDS             complement(7942..8808)
                     /locus_tag="U4A13_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1780031.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="U4A13_001749"
     CDS             8917..9558
                     /locus_tag="U4A13_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN1780032.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="U4A13_001750"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="U4A13_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN1780033.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="U4A13_001751"
     CDS             complement(10999..11898)
                     /locus_tag="U4A13_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1780034.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="U4A13_001752"
     CDS             complement(11900..12697)
                     /locus_tag="U4A13_001752"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN1780035.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="U4A13_001753"
     CDS             complement(12675..13379)
                     /locus_tag="U4A13_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN1780036.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="U4A13_001754"
     CDS             13510..14085
                     /locus_tag="U4A13_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEN1780037.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="U4A13_001755"
     CDS             14203..14880
                     /locus_tag="U4A13_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN1780038.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="U4A13_001756"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="U4A13_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN1780039.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="U4A13_001757"
     CDS             complement(16943..17941)
                     /locus_tag="U4A13_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN1780040.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="U4A13_001758"
     CDS             complement(18167..18820)
                     /locus_tag="U4A13_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN1780041.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DASJPC010000052        18735 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJPC010000052 DASJPC010000000
VERSION     DASJPC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.052"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            251..526
                     /locus_tag="U4A13_001759"
     CDS             251..526
                     /locus_tag="U4A13_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780042.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="U4A13_001760"
     CDS             539..2611
                     /locus_tag="U4A13_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN1780043.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2776..4662
                     /locus_tag="U4A13_001761"
     CDS             2776..4662
                     /locus_tag="U4A13_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN1780044.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4674..5621
                     /locus_tag="U4A13_001762"
     CDS             4674..5621
                     /locus_tag="U4A13_001762"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN1780045.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5641..6168
                     /locus_tag="U4A13_001763"
     CDS             5641..6168
                     /locus_tag="U4A13_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1780046.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6231..6884)
                     /locus_tag="U4A13_001764"
     CDS             complement(6231..6884)
                     /locus_tag="U4A13_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN1780047.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7016..7858
                     /locus_tag="U4A13_001765"
     CDS             7016..7858
                     /locus_tag="U4A13_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1780048.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8016..8912
                     /locus_tag="U4A13_001766"
     CDS             8016..8912
                     /locus_tag="U4A13_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN1780049.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8915..9628
                     /locus_tag="U4A13_001767"
     CDS             8915..9628
                     /locus_tag="U4A13_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN1780050.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9648..10166
                     /gene="msrA"
                     /locus_tag="U4A13_001768"
     CDS             9648..10166
                     /gene="msrA"
                     /locus_tag="U4A13_001768"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN1780051.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10163..10390
                     /locus_tag="U4A13_001769"
     CDS             10163..10390
                     /locus_tag="U4A13_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN1780052.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10542..11093
                     /gene="lepB"
                     /locus_tag="U4A13_001770"
     CDS             10542..11093
                     /gene="lepB"
                     /locus_tag="U4A13_001770"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1780053.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11176..11676)
                     /locus_tag="U4A13_001771"
     CDS             complement(11176..11676)
                     /locus_tag="U4A13_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN1780054.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11955..12353)
                     /locus_tag="U4A13_001772"
     CDS             complement(11955..12353)
                     /locus_tag="U4A13_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1780055.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12613..13473
                     /gene="ylqF"
                     /locus_tag="U4A13_001773"
     CDS             12613..13473
                     /gene="ylqF"
                     /locus_tag="U4A13_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN1780056.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13466..14233
                     /locus_tag="U4A13_001774"
     CDS             13466..14233
                     /locus_tag="U4A13_001774"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN1780057.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14289..15146
                     /gene="dprA"
                     /locus_tag="U4A13_001775"
     CDS             14289..15146
                     /gene="dprA"
                     /locus_tag="U4A13_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN1780058.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15268..17346
                     /gene="topA"
                     /locus_tag="U4A13_001776"
     CDS             15268..17346
                     /gene="topA"
                     /locus_tag="U4A13_001776"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN1780059.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17315..18691
                     /gene="trmFO"
                     /locus_tag="U4A13_001777"
     CDS             17315..18691
                     /gene="trmFO"
                     /locus_tag="U4A13_001777"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN1780060.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3898 g         5088 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     2641 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     2701 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     2761 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     2821 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     2881 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     2941 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     3001 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     3061 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     3121 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     3181 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     3241 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     3301 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     3361 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     3421 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     3481 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     3541 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     3601 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     3661 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     3721 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     3781 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     3841 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     3901 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     3961 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     4021 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     4081 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     4141 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     4201 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     4261 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     4321 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     4381 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     4441 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
     4501 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
     4561 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
     4621 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
     4681 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
     4741 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
     4801 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
     4861 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
     4921 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
     4981 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
     5041 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
     5101 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
     5161 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
     5221 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
     5281 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
     5341 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
     5401 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
     5461 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
     5521 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
     5581 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
     5641 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
     5701 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
     5761 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
     5821 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
     5881 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
     5941 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
     6001 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
     6061 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
     6121 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
     6181 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
     6241 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
     6301 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
     6361 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
     6421 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
     6481 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
     6541 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
     6601 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
     6661 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
     6721 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
     6781 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
     6841 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
     6901 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
     6961 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
     7021 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
     7081 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
     7141 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
     7201 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
     7261 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
     7321 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
     7381 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
     7441 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
     7501 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
     7561 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
     7621 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
     7681 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
     7741 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
     7801 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
     7861 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
     7921 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
     7981 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
     8041 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
     8101 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
     8161 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
     8221 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
     8281 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
     8341 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
     8401 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
     8461 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
     8521 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
     8581 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
     8641 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
     8701 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
     8761 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
     8821 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
     8881 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
     8941 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
     9001 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
     9061 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
     9121 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
     9181 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
     9241 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
     9301 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
     9361 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
     9421 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
     9481 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
     9541 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
     9601 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
     9661 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
     9721 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
     9781 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
     9841 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
     9901 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
     9961 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    10021 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    10081 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    10141 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    10201 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    10261 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    10321 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    10381 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    10441 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    10501 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    10561 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    10621 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    10681 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    10741 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    10801 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    10861 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    10921 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    10981 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    11041 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    11101 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    11161 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    11221 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    11281 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    11341 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    11401 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    11461 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    11521 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    11581 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    11641 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    11701 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    11761 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    11821 aaaagggtga gaagactgct tttataaact gtaaaaaaga gattgtacga ataaaaaaaa
    11881 tagaggcgga gaatacttat cgtatcatcc gcctttgttt ttttgttata aaaaacgtca
    11941 gattagttat ctgcttacat cttcatccat acgggttcta atgtatttcc gtctagatct
    12001 tggacttcta atgaaaacat ttcttcttca ggcattccca tatctacttc gtggaaagta
    12061 ccaccatttt cttttgcaat ttgagcaaat ttttttactt catccgcgtt ttcaacacta
    12121 aacgaaatca aagcaccact tgttttttta ttatcagcta tttctttatc ttttataaat
    12181 tttttataaa aatcatgaga gagaagcatt atccaaaaag tgtcatccca taccattgaa
    12241 ccagcttctt cagtagaaaa ttcttcattt tttttaaaac ccagcttttc ataaaaagca
    12301 acggaacgat tgacatcagc aacaggaaaa ttaacaaaaa ccatactatt catttgtttc
    12361 gtctcctttc tgttttgttc gtctatcatc tcttatttag gaaacgctgt caaaaattta
    12421 gattcatata tagaaaccaa aataaaaatt tgcatcatca aaaaagtgaa gatgtgatag
    12481 aatgaggact ggttcacttt cacttaacta atagatggaa tttctttata ataaaaaagt
    12541 gaatttatat tgaaaaagaa aaaaacaagt aaagccgggg aatattccgg tatgaaagga
    12601 atggagagaa caatgaccat ccaatggttt ccgggacata tggcaaaagc ccgacgagaa
    12661 gtttctgaaa aaatgaaata tgtggacatc gtatttgaat tggtagacgc acgattacct
    12721 ttatcttctc gtaatccaat gctagatcag atcattcagc aaaaacctcg ccttgtttta
    12781 ttgaataaag cggatctggc agatccacaa cagacaaagc tgtggcaaga atattttcgt
    12841 aaacaaggcc atcttgcatt agctatcacg gcacaagaaa gtaaaggaat taaaggtcta
    12901 gtacctgcgg ctaaagaagc attgaaagaa aaacgggcaa gagatgctgc tcgggggatc
    12961 aagcctcgcg ctattcgggc aatggtctta ggtattccaa atgttgggaa atctacgttg
    13021 atgaatcgac tagtaggcaa gaaaattgca cagacaggaa ataagcctgg cgtaacaaaa
    13081 ggtcagcagt ggcttcgttt aggtaatgag ttggaattgt tagacacacc agggatttta
    13141 tggcccaaat ttgaagatga agaaatcggc aaaaaactag cgctgacagg cgcgatcaaa
    13201 gaccagcttg tccatttaga tgatttagcg atttacggat tagacttttt tgcccgatat
    13261 tattcaggaa gaatcagcga tcgttatggt ttatcaatcg agcaagaaga aaaattggca
    13321 ccggaattac tgatggatat tacagcaaaa cgtgggtata aagaagacta tgatcgtggc
    13381 agcgaattag tcgtgcatga tattcgtcaa ggaaaacttg gacgatacac actggatcgt
    13441 tgcgaagaaa tggagcaaaa aagtgatgac tgaatccatc caaagtatca aagaacgatt
    13501 gaaaagcata cagagaatag atgatcctac actagaatca ttaagaaatg atccacgcaa
    13561 aggcgtgcag caagcactgg cttcttttga acgacgattg aaaaaggaag cattaattaa
    13621 ggaaaaatat gaagaaatgt tttgctttga aaagcaaggg tacgcacaag gtcaccaatg
    13681 gattgccggc attgatgaag taggaagagg accattagct ggacctgtcg tggcagctgc
    13741 agttatcttg ccgaaaaatg agatcatatt agggctgaat gactccaaac aattatcaga
    13801 gaaaaagcga gaacttcttt atcaacaagt tcaagaaaaa gccctggcaa tcgggatcgg
    13861 gatcgttgat cagcagacaa tcgatgatgt gaatatttat gaagcaagta aattagcgat
    13921 gatccaagca gtagaaaaat tgtccattgt accaagctat ttgttgatcg atgcgatgac
    13981 cttggatttg cctattcagc aggaaaaaat aatcaaaggg gatgcacgat ctgtgtcgat
    14041 tgctgctgca agtatcattg ctaaagtata tcgagaccaa ttgatgaaag aatatgacgt
    14101 gctttatcct ggatatggtt tctcgcataa tgcaggttat ggaacaaagg aacacttaga
    14161 aggacttgaa cgacaaggaa ttacgccaat ccatcgtaaa agttttgcac ctgtaaaaaa
    14221 atatttacaa taaaaaacag ccgcttttac gtatcttatt agaaagagag acgaaaggcg
    14281 gttttatgat gtatcaaata gaagaaaatt tattgaaact tgcttataca aaaggaatca
    14341 gtagtcaagg aaaatgggca cttgctaact ggatgatgca gtatccttat aaagaaatcg
    14401 gatttgaaga catcattaag ataggtaaaa taaccaaaca tcgggagctg tttcgcaaaa
    14461 gttggcagga aattcacgct aactggggca aaatccaatc gaaacagtcg tttatcactt
    14521 gctttgatcc caaatatccc cctcagctgc ttcacttgac ctatccgcca attgtactgt
    14581 tttataatgg tgatctatct ttactttctt gtaacatgtt aagcgttgtt ggtggaagac
    14641 acacttctgc tttagccaaa aaaacagttt attatttact aaaaccagtg atagaagctg
    14701 gatatgtgat cgtcagtggc tgcgcgaaag ggatagatac atacggtcac caagcagcaa
    14761 tctctcatgg tggacggaca atcgcagtca ttggaacagg gatagagcaa gtctatcctg
    14821 ctgaaaatcg tgacttgcaa gaggtgattg caaatgatca tcttcttttg agtgaatatc
    14881 ctcctcaatc cggcccgaaa aagcattgtt ttccaatgag aaatcggatt atcgcagctt
    14941 tgtctaaagg aacatgtgtc tttgaagcca agaaaaaaag cggctctctg ataacagcac
    15001 agcaagcatt ggatcttgga agaacagttt tttctgttcc aggaaatatt ttaacagaac
    15061 attctaccgg atgtcatcat ttgatccaag acggtgcagt ttgcacggtt tcgggtgaag
    15121 atatcttggc agaatttaaa gaataacacc atacttttca tttgcttttc ttgacaaacg
    15181 ttttctgaat ggatatgatt agctacgatt tgtatgaatc ctcttttaat agtaggaaca
    15241 cgacaatgga aaggagtcca ttcagtaatg gcgtataaat atttagtgat tgtagaatca
    15301 cctgcaaaag ccaagacaat tgagaaatat cttggacgaa actataaagt aatggccagt
    15361 gttggccata taagagattt accgaaaagt aaaatgggta tcgattttga aaacaattat
    15421 gaaccccatt atatttctat acgcggaaaa ggcgatgtca tcaaaagcct gaaagccgca
    15481 gcaaaaaaag ctcaaaaagt ttacttggca agtgacccgg atagagaagg agaagcgatt
    15541 gcttggcatt tagcgtacct acttgggttg gatctgaaag aaaaaaatcg ggtggtcttc
    15601 aatgaaatca caaaagacgc agtcaaagca gcttttaagg aaccaagaac gatcgatgta
    15661 gatttagtag atgcacagca agctcgtcgt accttagaca gaatcgttgg ttattcgatc
    15721 agtcctattc tctggcgtaa ggtcaagaaa gggttaagtg caggacgtgt ccaatctgtc
    15781 gctttaaaaa ttattattga ccgtgaaaaa gagatccgag aatttgttcc agaagaatat
    15841 tggagcatcg acggtaattt taaaaaagct cgcaagaaat tcaaagcaaa tttctgggga
    15901 atcgacggta agaaaaagaa attaccagat gcacaaagtg taaaagaagt cactgctaga
    15961 attgatggaa cagacttcga agttaaaaaa gtagaaaaga aagagcgcaa aagaaaccct
    16021 gcgaatcctt tcactacaag tagcctgcaa caagaagctg cccgtaagtt aaactttcga
    16081 acaagaaaaa ccatgatggt agcacagcaa ttatatgaag gaatcacttt gggtaaacaa
    16141 ggaacagtcg gcttgattac atacatgcgt actgattcta cccggattgc cgatacagca
    16201 aaagcagagg ccgcagcatt tattgaagat acgtatggaa aagaattttc tagacatgat
    16261 caccgaaaga tcaaaaatgc tcaaggcgca caagatgccc atgaagctgt gcgtccaacg
    16321 agtgtgcttc gtacaccaga tgaactcaaa caatatttag ataaagatca attgaaactt
    16381 tatacattga tctggtcacg ttttgtggct agtcagatga caccagctat tttagataca
    16441 atgaaagtga cattagaaca aaatggtgtc atcttcattg cgaacggttc aaaaatcaaa
    16501 ttcaaaggat tcatgcaagt gtatgtggaa ggtagagatg acggcaaaga agagaaagaa
    16561 aatattcttc cagaacttga agaaggcgat gtagtccaat ctgttgatat tgaaccaaaa
    16621 caacatttta ctcaaccacc agcacgtttt agtgaggcta cgttgattcg tgcactagaa
    16681 gaaaacgggg tagggcgacc ttccacttat gcaccaacac ttgatacgat ccaaagacgc
    16741 tactatgtaa aattgacaca aaaaagattt gagccgactg aactaggaga aattgtcaat
    16801 tcattaatct gtgaattctt ccctcaaatc gtcgatatcc atttcacagc agaaatggaa
    16861 ggagatttgg ataaaattga agaaggcacg gaagcgtggg tcaaagtggt tgatcgtttc
    16921 tataaaccgt ttgaaaaaga actgacgaat gcggaagaaa aaatcgaaaa aattcaaatc
    16981 aaagatgaac cagcaggatt cgattgtgat gtctgtggac atccgatggt gataaaatta
    17041 gggaaatatg ggaagtttta tgcttgcagt aattttcctg attgtcggaa tacgaaacca
    17101 atcgtaaaag aaatcggtgt gacttgccca gtatgtcatg aaggacaagt cattgaacga
    17161 aaatctaaga aaaacagact attctatggt tgttcccgtt tcccagaatg tgaattcact
    17221 tcttgggata aaccagtggg cagaaattgt ccaaaatgtg acggatatct ggtcgagaag
    17281 aaagtcaaag gcggtaaaca agtagtctgt gtaaatggag attacgaaga agacatccaa
    17341 aaataataga aaagaggttc tttcattgaa aatggtaaat gttgttggag cagggcttgc
    17401 cggaagtgaa gctgcttggc agatcgcaca agcaggagtc cctgtccgtt tatatgaaat
    17461 gcgtccagtc aaatcgactg aggcgcatca cacaagtaat tttgctgaac tagtatgttc
    17521 caattcatta agaggaaaca gtttggctaa tgcagtaggg ttattgaaag aagaaatgcg
    17581 ccgattgaat tcggtagtaa tcacttcggc agatgaaaca gcagtgccag ctggtggtgc
    17641 cttagctgtt gaccgcgatt ctttttctga aacaattacc cgaaaagtca aagagcatcc
    17701 attagtaaca gtgataaacg aagaaattac tgaaataccg gaaggaatca ccgtagtagc
    17761 aacaggacct ttgacttctc atccattagc agaaagcatc aaagcattca atggttcaga
    17821 tggtttttat ttctatgatg cagctgcacc aatcgtagac aaagctacga tcgatatgga
    17881 taaagtatat ttgaagtccc gctatgacaa aggagaagca gcttacttga actgtccgat
    17941 gacagaagag gaattcaaag ctttccatga ggcactaatc aatgccgaag tggcagaact
    18001 taaatctttt gagaaagaaa aatattttga aggatgtatg ccaattgaag taatggcaca
    18061 gcgaggattc aaaacgatgc ttttcggacc tatgaaacca gttggattgg aagatcctaa
    18121 aactggcaaa cgtccttatg ctgttatcca gctgcgtcaa gacaatgctg cagcttcttt
    18181 atacaacatc gtgggctttc agactcactt gaaatggggc gagcaaaaac gtgttttccg
    18241 gatgattcct gggttagaaa atgctgaatt cgttcgctat ggtgtgatgc accgaaacag
    18301 ctttatgaat tcgccagaat tgctgaaacc gacttaccaa tcaacgaaac gcgaagatct
    18361 gttctttgca ggtcagatga ctggtgtaga aggctatgtt gaaagtgcag cgagcggact
    18421 acttgctgga atcaacgccg cacgcttagc aaaagaaatg gaaccagtcg agtttccaca
    18481 agaaacagct attggaagta tggcttacta cattactcat gcggaaggaa aacatttcca
    18541 accgatgaat gccaactatg gtctgtttcc tgaactgcca gagcgcattc gagacaaaaa
    18601 agcaagatat gaagcaattg cacaacgagc tttaaaagct aatgaggcaa taaaaacaga
    18661 attagaaaaa actgcaatcc caaatgagta aggaaactcc atgaagaggg tgagacggat
    18721 agtctcgctc ttttt
//
LOCUS       DASJPC010000053        17817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJPC010000053 DASJPC010000000
VERSION     DASJPC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.053"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            259..2730
                     /locus_tag="U4A13_001778"
     CDS             259..2730
                     /locus_tag="U4A13_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1780061.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2833..4569)
                     /locus_tag="U4A13_001779"
     CDS             complement(2833..4569)
                     /locus_tag="U4A13_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN1780062.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4566..5270)
                     /locus_tag="U4A13_001780"
     CDS             complement(4566..5270)
                     /locus_tag="U4A13_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1780063.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5401..5904
                     /locus_tag="U4A13_001781"
     CDS             5401..5904
                     /locus_tag="U4A13_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN1780064.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5864..6202
                     /locus_tag="U4A13_001782"
     CDS             5864..6202
                     /locus_tag="U4A13_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN1780065.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6223..7641
                     /gene="ffh"
                     /locus_tag="U4A13_001783"
     CDS             6223..7641
                     /gene="ffh"
                     /locus_tag="U4A13_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN1780066.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7753..8331
                     /locus_tag="U4A13_001784"
     CDS             7753..8331
                     /locus_tag="U4A13_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN1780067.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8335..8856
                     /locus_tag="U4A13_001785"
     CDS             8335..8856
                     /locus_tag="U4A13_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN1780068.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8935..9489)
                     /locus_tag="U4A13_001786"
     CDS             complement(8935..9489)
                     /locus_tag="U4A13_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1780069.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9742..10017
                     /gene="rpsP"
                     /locus_tag="U4A13_001787"
     CDS             9742..10017
                     /gene="rpsP"
                     /locus_tag="U4A13_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN1780070.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10029..10280
                     /locus_tag="U4A13_001788"
     CDS             10029..10280
                     /locus_tag="U4A13_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN1780071.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10405..11196
                     /locus_tag="U4A13_001789"
     CDS             10405..11196
                     /locus_tag="U4A13_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780072.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11366..11887
                     /gene="rimM"
                     /locus_tag="U4A13_001790"
     CDS             11366..11887
                     /gene="rimM"
                     /locus_tag="U4A13_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN1780073.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11877..12638
                     /gene="trmD"
                     /locus_tag="U4A13_001791"
     CDS             11877..12638
                     /gene="trmD"
                     /locus_tag="U4A13_001791"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN1780074.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12774..13121
                     /gene="rplS"
                     /locus_tag="U4A13_001792"
     CDS             12774..13121
                     /gene="rplS"
                     /locus_tag="U4A13_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN1780075.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13253..14389)
                     /locus_tag="U4A13_001793"
     CDS             complement(13253..14389)
                     /locus_tag="U4A13_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1780076.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14505..15419)
                     /locus_tag="U4A13_001794"
     CDS             complement(14505..15419)
                     /locus_tag="U4A13_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEN1780077.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15510..15938)
                     /locus_tag="U4A13_001795"
     CDS             complement(15510..15938)
                     /locus_tag="U4A13_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN1780078.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15943..16317)
                     /locus_tag="U4A13_001796"
     CDS             complement(15943..16317)
                     /locus_tag="U4A13_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1780079.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16603..16788
                     /locus_tag="U4A13_001797"
     CDS             16603..16788
                     /locus_tag="U4A13_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1780080.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16800..17048
                     /locus_tag="U4A13_001798"
     CDS             16800..17048
                     /locus_tag="U4A13_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780081.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17079..17258
                     /locus_tag="U4A13_001799"
     CDS             17079..17258
                     /locus_tag="U4A13_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780082.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17301..17771)
                     /locus_tag="U4A13_001800"
     CDS             complement(17301..17771)
                     /locus_tag="U4A13_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780083.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
BASE COUNT         5790 a         3156 c         3491 g         5380 t
ORIGIN      
        1 ggtaggatga cgttttggtc gactaaattt taccaaaagt gagtctcttt ttgagttgaa
       61 acgaagaaaa agtgttagta gaaaatcgcc ttcgattttc caccaacaca aaatattata
      121 gaaccctttt tttttgctat atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga
      181 acactttcca tatctgttct atactggata ggagaagttt ggaaatatcc agaaaacgaa
      241 atcaatcgag gtagccacat gaagaataaa tggataaatc gaagtaaaaa aaagaaagta
      301 acacaaagat catcagataa agtcatccgt ctttttccat cgtataaaga aggattaacg
      361 gcagatcaag tcaaggaacg aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca
      421 ttcaaaacaa atcagcaaat cgtattggaa aacatattta cgtatttcaa tttgatcttt
      481 cttatcttag ccattttgct ttgtttagta gaatcctata agaacttgac ttttttacca
      541 gttatcatag ccaatacagg aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg
      601 gacgaactaa atgttttaaa tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa
      661 acaatagata tagaagacct tgtaatagat gacattgtta ttttagagac tggatatcaa
      721 attccagcag atgcagaggt cgtggaaggg aaactgcaag ttaatgaggc tttattgaca
      781 ggcgaagcag atgaaataac caaagaaata ggtgatccat taatgtccgg cagtttcatt
      841 gtttccggaa aagcttacgc ccgattagac aaagttggag cagattcgta tatttcccag
      901 ctgacagtca aagccaagac aatgggagaa ggagaacagt cggagatgat tgcttctctt
      961 aatcaattga tcaaatggat tgggatcatc attattccta tcggtatcct gcttttttct
     1021 caaagttact tttttaatgg aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca
     1081 ttgatcggta tgatacctga aggactgtac ttgctgacga cgattgcatt agcattaagt
     1141 gcgactaaac tagcaaagca aagagttctt ttacacaata tgaaaagcat tgagacattg
     1201 gcaagggtaa atgtcctttg tgtagataaa acaggaacga tcacagaaaa taaaatgtct
     1261 gttcaaaaaa tgatcgtctc taaaaaacaa gaaataatca gtcagtcaga tcaattggaa
     1321 gcatgcattg ctgactatgc aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt
     1381 aaagcatatt ttacaaaaac cactgatcaa tcctatcacc atgttattcc tttttcttct
     1441 gtccaaaaat tcagcagtgt agcgttggca gacaaggtgt atgttttagg agcgcccgaa
     1501 atggttttgc gcgatcaatt gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa
     1561 ggctatcgcg tgttggtctt tggtgtgtac gatgggattc ttgaagaacc agtattgaca
     1621 gaagcagtca caccattagg ctatattctg attgctaatc ctatcagaaa agaagcaaga
     1681 gccacatttg attacttcaa aaaacagcaa gtagccatca aagtgatttc tggagataat
     1741 ccgctcaccg tatccaatgt agctgtccaa gcagggattt cacatgctga tcagtacgtt
     1801 gatgcatcta aattatcaga agaagaatat gaagcagctg tcgaaaaata tacagttttt
     1861 ggacgcgtca agccagaaca aaagaaaatc ttcgttcaat tgataaagcg taacaataca
     1921 gtagctatga caggtgatgg cgtgaatgat attctagcca tgaaagaagc agactgcagt
     1981 atagcgatgg cttccggaaa tgaagccgcc atgcaggcct cacaagtggt cttacttgat
     2041 tctgactttt cacgcatgcc agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt
     2101 gagcgatcag ccagcttgtt tttagtgaag aatattttct cctttttgct ctcactgttt
     2161 tcagtgatct ttgctctgac ctatccttta gaaccatcac aaatcacatt gattagtttg
     2221 tttactatcg gacttccatc tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga
     2281 gggaaattca tcatgaatgt gatggaaaaa gctgtgccag gtggactaac agatatgatc
     2341 gtagtcggtg cgttggtgat ctgcggggtg actttgaatc taaataaaac agatgtttct
     2401 acagcatcta ccatgctttt gattgcagta gggttcctcg ttctctataa aatctgttca
     2461 ccgctgaata aattcagaag tcagatcatt cttttctgtg ctagcggtaa ctttttttcg
     2521 gtcatctttt tgcataagct gttttcaatc accggtatct cagctgtatc acttttgctg
     2581 ttgagcattc tatttttctc ggcagattcg atttttaggc atttgaccac attggtagaa
     2641 tatttgtttg aacgtaaaaa tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa
     2701 tttctatcga tatttaaacg agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag
     2761 aacaaccact cgtgtatctt tcgaagcagg atgagtcatc caactttaaa acgcacgagc
     2821 agttgctctt ttttacattt tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg
     2881 actgtctaca gtaatcgtac cattaaggag ttgcacgtag tcttttacaa tcgctaaccc
     2941 taatccggtc ccgcctgaat gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa
     3001 gatccgttcc tgatcctcaa tgtcgatacc tataccaaag tcttggacag aaaacgatag
     3061 atcatctgtt gcctgatagc ggataataat ctcgctgtcg ctttttgaat attggacagc
     3121 attttcaatt agatttttga taattggata aaggatatct gttttagtag taaatggttc
     3181 gttctttcca atcaatcgaa tcgtcaaccg tttcttttcg atgattggct gataattttg
     3241 aacgatttgc tggaaataag gttctgtata aattgtctgg atctcgtaag aattctcgcc
     3301 atcttttgac agctggatga tttcccgaat cagtttatct aaccggatcg catctttttg
     3361 catgatctca agaaaagaag tcagagtttg cgggtcatct tttgcaccat ccagtaaagt
     3421 ctcggtaaaa ccaatcagtg aagtcacagg agtttttaat tcgtgagaga cgttgccaac
     3481 gaaatccttt tgtagctttt ctaaacgtcg cactttcgtt aagtcatacg ccacccctag
     3541 aatctgtccg gtaccttctg aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg
     3601 accttctgta agggtgatct cctgatggat cagcggatgc tcaggagtca cttgatgaat
     3661 cagctgaatg agttttggct cttgaatgac ttctgtgtac cgctgttcag gttgatagct
     3721 taccacgttt aaatgtagct gcatctttgg attaagcagt agtaaacgag aatcagcatc
     3781 atcaatcagg aatacgccaa tcatcagttc gttcagtaac gtatataatt gctcttctgt
     3841 ggtagtacac gcccgataca tcttactcat ttgttcgctc aacgcattaa tcgtctgata
     3901 caattcttcc cattgatcgg gtgtttgcat gattacttcc gcctgattag gattttgcac
     3961 catttttttt agtacgggca gcacggtttt caatggctgg tttttttgat agatcagata
     4021 atagatcatt cctgcaagaa caaggaagaa agacagaaag aagaaaaaga cccaacggcg
     4081 aaaactctca gttctcggta aaaaaccgct tgtcggttct gcgatacgta aaatggcttc
     4141 taactggcca ctttttttga ctggtaatgc gacatataaa agatcctcat tcaaagtagt
     4201 acttttgcgc aaagcgctac ctaggttccc accattcaat acggctttga tttcgggacg
     4261 ggagtttcgc tgttcattca atgtttcatt gtttgtgtca aataagatct ttccagtagc
     4321 atccattaaa gtaatccgtt cgttcgactg atggacgaat tcttccaaag cagttttgtt
     4381 ttgcgctgct tggaggtttt cgacatctaa ttgccggatc agcagttgtg ctttttttgt
     4441 caggtagctt tcttgctgtt ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca
     4501 gccaccaaaa aacaaactaa ctaaaagaat aagaaagaag agacgttcat aaattttttt
     4561 ccatttcatc gtttgggttc ctgaaactta tagccaaatc ctctaatagt caccaagtac
     4621 tttgggtgtt ttggatctcg ctcgattttt tctcgcaaat gactaacgtg tacatctacg
     4681 attcggcttt gtcctgcgaa gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg
     4741 atcacacggt cttttcgttt catgaaataa acaagtaatt caaattcttt cggtgttagt
     4801 tcaatcagat ggtcgtctac ggtcacttga taattggtca aatcagcagt gatttgtcca
     4861 atcgatagat attcaggctc agcttcatct tttgattgct tacgaggttc aattcgtctg
     4921 aatattgctt tcatacgggc taatacttcg cgaggactaa atggtttggt caaatagtcg
     4981 tcagcaccta tttctaggcc aatgatccga tccacttggt catctttagc cgtcagcatc
     5041 aaaatcggtg tttcattttt ttcttgacgc agccgctgag tgatctccat gccatccatc
     5101 gttggaagca tcacatctaa aatgatgaaa tcaaatgatt gttctaatgc taattctaat
     5161 ccttcgcctc catctgtggc actggttacc ttgtagcctt ctttttccaa attgaacgtc
     5221 aagagcgtta caatagaagg ttcatcatca acaactagta tttttttcat tcaaatctcc
     5281 tttaaatagg tttcattctg tttatatttt agcatagaat cagtacaaag ggctatttta
     5341 caaaagaaat aaatggtata ataggaaggc actttgctga atagaaagga agagcagtca
     5401 atgattggaa tcagtgcatg tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa
     5461 gaagtaccag aattgatgga actagttgaa actggagaag ctgtcatggt ctgtccagaa
     5521 gtgatcggcg gattaccaat tcctagagat cccgctgaaa tcattggtgg aaatggattc
     5581 gatgtgtgga aagatcaagc aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg
     5641 tacaaaaatg gcgcgatcaa agcctatcaa aaattgcagg aaaaacacat tgatctagtc
     5701 atcatgaagg aaaaaagccc ttcatgcgga acccattcga tttatgacgg aagtttttcc
     5761 ggaataaaaa aaaatggtgc cggagtggct actgcttatt ttattcagca aaaaatgact
     5821 gtgctctctg atgaagaatg gctgaaaact agaggtgagc agaatggaaa tcgaaaagac
     5881 caatcgaatg aacgcgctct ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa
     5941 ctacatggaa ttatattatg cagatgattt ttcattaggt gagatcgcgg aagaatacga
     6001 agtaagccga caagcagtat atgacaatat caagcggaca agcaagatct tggaggatta
     6061 tgaaaaaaaa ctccatcttt tttctaatta tattgttcga gaacaggtat tggaaaatat
     6121 gactgcatat attacagata agtacccgga agataaaaaa ctacttgaat atgtacagca
     6181 aattcaagca atcgaagaat aaataaaagg aatgaagaaa atatggcatt tgaaagctta
     6241 actgaacgtc tccagcaggc gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct
     6301 gacgtaaaag aaatgatgcg ggaaatccgc cttgccttac tagaagccga tgttaacttg
     6361 caagtagtca aagattttac taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg
     6421 gaaagtttat cacctgcaca acaaatcgta aaaatcgttg atgaagaatt gacgatcaca
     6481 cttggttctg aaacagcaga gttaaataaa tcaccaaaaa taccaactgt tatcatgatg
     6541 gctggtcttc aaggggcagg taaaacgacc tttactggta aattagccaa ttatttaaag
     6601 aaaaatgaaa atgctcgtcc gctcctaatc gcaggggacg tatatcgacc agctgcgatc
     6661 gaccaattga aagtattagg tcaacagcta gacgttcctg tatttgatat gggaactgaa
     6721 gtcagcccgg tagagatcgt ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac
     6781 tatgtattga tcgatacggc aggacgtctg catatcgatg aaacattgat ggatgagttg
     6841 aaacaaatca aagaattgac gcaaccaaat gagattttgc ttgttgtcga tgccatgact
     6901 ggtcaagatg ccgtcaacgt agctgatagc ttcaaccagc aattaggtat tacaggggtc
     6961 gtcatcacta aacttgatgg ggatactcga ggcggggcag cactatcgat ccgctcagtg
     7021 acaggcgcac caatcaaatt catcggttct ggtgaaaaat tgactgactt agaagtattc
     7081 cacccagatc gtatggctag ccgtatcctt ggtatggggg atatgctgac attgattgaa
     7141 aaagcgcagc aagactacga cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa
     7201 aattcattcg atttcaatga ctttattgaa cagcttgatc aagtgatggg tatgggccca
     7261 ctggaagatc taatcaaaat gatcccagga atgaatcaag tccctggaat cgaaaacgtc
     7321 aaggtagatc caaaagatgt tgaacgcaaa aaagcgatgg tttattctat gacgccagca
     7381 gaaagagcaa atcctgatct gttgaaccca agccgtcgtc gccgaattgc tgccggttct
     7441 ggaaacagtg ttgttgaagt gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg
     7501 atgcagcaaa tgtccaaagg ggatatgaac atcccaggta tggaccaaat gtttggttca
     7561 ggcgtcaaag gaaaactagg caaaatggcg atgaatcgaa tgatcaagaa aaataagaag
     7621 aaaaagaaaa aacggaaata agatgaaaaa aacagcttcg gtacagactg aaaatgtcta
     7681 ctgccggagc ttttctatca gcaattttta ttctttacta atacaattac tctaaacgaa
     7741 gaggtggcac agatgagatg tccgtggagt gaacaaacag aaagtatgcg gcaatatcat
     7801 gatactgtct ggggtgttcc tgaatatgat gaccaaaagc tgttccgaaa attgatgctt
     7861 gatattaatc aagccggatt gagctggcag acgattttaa ataaaagtga agcattcgat
     7921 gaagcatatg atcgattcga tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt
     7981 gaactaatgg aaaacaaagg aattattcgt aaccgaagaa aaatcgaagc agccgtccaa
     8041 aacgccaaag cagtcttgaa aattcaagaa gaaatcggca gtttttcaga gtatctttgg
     8101 tcgttttcta atggaaaaat cgttgattct tcttatcatt cacaagaaga agtcccaaca
     8161 acaagtcctc tttcagatga attagcaaaa gatctaaaga aaagaggatt taaatttatc
     8221 ggaagcacaa caatctatgc ttttttagaa gctgtcggga tcatcaatga tcaccttgac
     8281 agttgtttcc gcaaagaaga ggtcgtacgt atcggcagaa agggcgaata aagcatgcga
     8341 aaaatcatat tttatggtgc tataagttta gatggctatt tggcaacgaa aacggatgat
     8401 cttcaatggc tttttgacac tccaacaggt aagcaaacta cttatgaagc tttttatgaa
     8461 acaatcgata cgaccatcat gggcagaaaa acttatcagg aagcaaaaaa atatttggag
     8521 accgaagcaa tttatccaga gaaaaagaat ttcgtttttt ccacccacgc acaattacaa
     8581 ttgccagatg ctgaggtcgt aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg
     8641 acagaaggaa aagatatctg ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa
     8701 catcaaatga ttgatgaatg gtatatccag attacccctg tattattagg ggacgggatt
     8761 cctttattcc aaaaaggaac atatgaagcg cgttttgaaa tgctagacac gacacgattc
     8821 ggtgaattta tcgaattgca ttatgtacga aagtagaagt aaataaaata aaaaaggcaa
     8881 ctatatgcag aaatgtcctg tacatagttg ccttttttat taccgttatg ctttttactc
     8941 tgttaatttg ttagaaaaat ctagatacgc atctaaaatc gtttggaaga aacttaatgt
     9001 tccttcaacg atattaccat tttcatcaat tagattcgca acgtttccga tatatgcttc
     9061 cggttgctgc aaggttggca cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc
     9121 gccaaagccg ccgatcccac ctggtgatgc agtaacgact aaacctggtt tatgatccca
     9181 aacactgtgt ccataaggtc ttgagcctac atctaaagca tttttcaata ctgctggcac
     9241 agaacggttg tattcaggag taacgaaaac gacaccgtct agtcccctca cttcctcacg
     9301 aaaacgagtc cattcagcag gagcctgttc tggagtttcg agatcctcat tgtaaaatgg
     9361 cagatcatcg atttttacaa atacactttc gtacccttca ggaagcaatt tcgcaaaagc
     9421 ttctgctact tttttgttgt aagaatcttt tcttaaacta ccaataaaaa atccaatttt
     9481 ttttgtcata cgatttttcc tccttcaaac tatctataat aatcttacaa aagataagta
     9541 actggagcaa ataaaaacac ttactgacaa aagctacata gaagatagat tacttatttt
     9601 taacaaaaaa gaagtctaaa atcttctatt ttctaaaaaa aatcgaattt aatcaagctg
     9661 taaagaaaaa aagctttaca gggtaaaaaa aaactgttac actacatctt gtgaataaaa
     9721 caatggaggt ggaaataaat aatggcagtt aaaatccgtt taaaacgtat gggttctaaa
     9781 aagagtccgt tttaccgtat cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc
     9841 atcgaaactg taggtactta caatcctttg aaagaccctg cagaagtagt tttaaaagaa
     9901 gacttagttc ttgactggtt atcaaaaggt gcacaacctt ctgacactgt acgtaacatc
     9961 ctttcaaaag aaggcgttat gcaaaaacac cacgaagcta aattctcaaa gaaataaggt
    10021 gaccggatat gaaagattta agcgacttag tcttaacaat cgttcgtccg ctagtcactt
    10081 atcctgatca agtcaagtta gacgtcgtag agtcgaaaga cttttacgag tacaatctaa
    10141 cagtggctcc tgaagatatt ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa
    10201 tccgcacaat cgtttacggt gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca
    10261 tcgatgataa ggaaaagtaa ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt
    10321 gtttttttta ttaattgaag aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt
    10381 ataggaggag ggagtgaaca gccaatgaga aagaaaatgt tatttattcc atttgctcta
    10441 ttgttaagcg cttgtagttc ggttactcaa gaatcagaca atacacatct atcaagcagt
    10501 atatctgaaa ctgttaccag tccagaaaaa agtagtacca aaacggcttc caccactgaa
    10561 caaacaatgg ctacctcaaa taatgagaaa aagactgctt tagaccagtt gaaagaacaa
    10621 cagccaaatg ttcccatgcc tctagatgta ccagtctctt caggctatct caatatcgca
    10681 gcgacacata caaagcaagg ttattctatt ttatattaca gaacagatcg tcccctcggg
    10741 ttgaatgctg acgaattgaa tcaagaaacg cctattgcaa cctatctata tcaatatggg
    10801 tttgcttcta gtcaagaaac aatccaagtt ttgcagccat ttgagatcga cacaaatggt
    10861 cagcaagtgg acttaggttc tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt
    10921 tttcttgaat ggcaagaagg aaactggcgt attcgaatcc gtgggaataa tatagaaggc
    10981 caagatccct tactattagc taaagaaatc gtagcttatt tggaagaaaa cagtctacca
    11041 gcacctgaac aatttggtaa aattactgtt gatatgggtg atacgacgaa tcgagccgta
    11101 gaagtaagct ggcaagaacc gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct
    11161 gcgttgaaga tggcagtttc tatgaagagg ttgtgaaatc agcgcgctga tctgagccca
    11221 tcatttaact ccaagtcaca ggaagtaatc aaaaatagca cgccgcattt ttgcataatt
    11281 ttggcttaat accttaaaag aaaactagaa ttgcctcttc acacatgcta aaataaaaca
    11341 gacaaaaaag aaatggggat ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa
    11401 cacacaaggt atcaaaggcg aagtccgtgt tatctcaaca acggatttcc cagaagaacg
    11461 ttacaaaaaa ggaacggtgc taacactatt ccaagacgga aaagcacctg tggaattaac
    11521 agtcaaaagt catcgcaaac ataaaaattt tgacttattg agttttgaag gacatccttc
    11581 tatcaatgat gtggaaaaat atcgggatgg catattaaaa gtgtcaaaag acaactctgt
    11641 agatctagct gaaaatgaat tctattacca tgagatcatc ggtctgcatg tattagaaga
    11701 tggaactgag ttaggtaaag taaaagaaat cttgtcacca ggtgccaatg atgtttgggt
    11761 cgttcaacgt ccgaaaaaag ctgatatatt gcttccatat atcgcttctg tcgtcaaaga
    11821 agtcgattta gaagctggag tggtccgtgt agaaattcca gaaggactga ttgacgatga
    11881 agattgatgt attgacttta tttccaagaa tgtttgaagg tcctatgggg gaatccatca
    11941 tcggtaaggc tgtagataaa ggattattag atatcaatat ctctaacttc cgcgattatt
    12001 cagataacaa acatcagaca gtagatgatt atccatacgg cggtggagca ggtatgctgc
    12061 tcaaagtcca gcccatctat gataacatcc aagccattga aaaagctgca ccggatgtca
    12121 aaaaaagagt aattcttctt gacccagctg gtaaacgatt cgatcagaaa atggcagaag
    12181 aattttcaca agaagaacac ttagtattca tttgtggtca ttatgaggga tatgatgaac
    12241 gtatccgcac attagtgaca gatgaagttt ccttaggaga ttatgtcctt actgggggag
    12301 aattaggtgc gatggtcatg atcgatgcaa cagttcggct tttgccagat gtgttaggga
    12361 atcagacttc agctcaaacg gattcttatt caaccggttt attggaacat ccccaatata
    12421 ctcgcccggc agaatataaa gggatgaaag taccagaagt attgacaaat ggaaatcata
    12481 aattgattga agaatggcaa ttaaaagaat cactaagaag gacctatcaa cgtcggcctg
    12541 atcttttgga aacacttgaa tggacacctc aaatgacgaa atttttagaa gaaatcaaga
    12601 aagaagaaca agaaaaaaca gcagatgaag ccaagtgact ttttctaaaa tcttctttac
    12661 agtaggttga taatatgtta gtattttatg gtgagtgcaa aagcacttaa ctattacgat
    12721 attccgctgt gagaaaagct cataagaata tttggaataa ggagaagaaa accatgaatc
    12781 cattaatcga agaattaaca aaagaacaat tacgttctga catcccagcg ttccgccctg
    12841 gtgacactgt gcgtgttcac gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat
    12901 ttgaaggtgt tgttatcaaa cgccgcggtg ctggaatcag cgaaacttat acagtacgta
    12961 aagtttctaa cggagtgggc gttgaacgta cattcccatt gcatacacca cgtgttgcaa
    13021 aaatcgaagt agttcgctac ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat
    13081 tacacggaaa agctgctcgt atcaaagaaa tccgtcgtta atcatcgtta tcagactgag
    13141 aaagaaccct tggtattcaa gggttctttt tttgttctct gtttttttat attaacgatt
    13201 tctatttggg acatttttga ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt
    13261 tttctatatc ttctttcaat ttttctttta ctccctttgt cacatgcaaa taaatctttt
    13321 cagtgatatc actattttcg tgcccaacgc gatcctgaat agcatacaga ggagttccta
    13381 gttccgctaa ttttgaaata tgggtatgtc taaaaatatg tgaactaagt tttttatcaa
    13441 tcttcatgtc agctttatga tttctcaaat aggtgttaat tgctgttagt tggaaaggag
    13501 ttcctttcgt tgtttgaaat agaaattgac cattcggatt taattctaga agctcattgt
    13561 aaatagctat cgcttttttt ggtaaatcaa tttctcgcat tccagcagca gtttttgtag
    13621 aatcagattt tttcatatca gctattgaac gttctctata catcatcgtc ccatttataa
    13681 ctactgatgc attattattg gtaatgtgta catcgtcttt acttaatgcg attgcttctc
    13741 caggcctcat tccagtcaaa tatagccact ggaaaaggag agaatatctt ttattgtgtg
    13801 aagttgtgaa atttactagt cttttatatt catcatcttc taaaaactta tctttaattt
    13861 ttatcgtttt tgactctcgt ttataatcaa taactacttc gttgatagga tttttttcta
    13921 catagccttt tttcatagcg tatgaaaaaa gaagattcaa tttggattta attacactga
    13981 cgtacttatt tgacaagtca tctttatata tcatatcttc aaaaatatta tttaaatcta
    14041 ctgtattaat accagaaacg atgtaagtct caggtatctt tttctttata gtgtttaaga
    14101 tattattcgt aggataatat gtagactcct taacttgtct tttgtaaata actaaccatt
    14161 cttcgacaag ctcatgaaat gttagatcag gcttctgaag tgttttttcg tttaatttta
    14221 tatcaatttt tttatttaac tctagcaatg ccactttctg agtttctcga gatttatttt
    14281 tataagtaat gctcactttt tttctttttc tagtttttgg atcaatatat cgctcattat
    14341 atttatatac tttttttccg tttttatcag ttttggtttc aatccacatt gttttcatct
    14401 cctatttgtt gctataatag gaatggataa gtaagccaat tatagcaggt ttatttttca
    14461 tcacgtccac aaactttggc gagggagggg gcgtgttttt ttgtttagta gctacctttc
    14521 catgaagtat ctgcgaagaa cacttcaaat gttgttccat catttttcac ttcaaaatat
    14581 gctgttcctt gtgcggattt acccggttgg atggtttccg aaaagtaatc tctactagct
    14641 aaatcagatt taatatctga tgaatcataa aattctaaat aacttgaact tgcatcaaaa
    14701 ggtgtagtcc ctgtgttttc tacaagaaaa tctacctttg cgtaaaatag gccttctggt
    14761 ttatagaaat catcaccgta atcttttgat acagactgga cagttacgtt aatagagtta
    14821 ccattttcag aagtaaaagg aactgcttct cctatactat gtgcaactga tgccgtctgg
    14881 ggttctgaag acgaagttgt agttgtagtt gtagtagagc ttgaatcttt ggattttcca
    14941 tctttatcta agaaagttcg ttgattataa tatacaacct gttcagtttt tggactaacc
    15001 caaattaaca ttgtttcatc atcagaatca gttgaataag tatattggag catctctaat
    15061 ttttttatat cttttgcact atttccatgt tcaattaatt cagagtgctc tttataataa
    15121 tctccttgtg catcctttga aatagataca attaattcat cccgtgtgat agcattgtct
    15181 aattctttgt aaacaaaatc atttgtccat ttacgtaacg gtgaaccaag tgtctgataa
    15241 acttgagtta aatcatcacc aacttttatt ttatgaacgt ctgatgaagt tagttttttt
    15301 gtttcatatt cagatgttga tgaaacagta ttttctttag tgctagcaga attaggacta
    15361 gtggagcatc cgcttaatac caagcctaaa aaaagcaacc caaaaacaat ttttttcatt
    15421 tttaattcct cacttctatg atatgatttt tttgtaggat cttagaaata aggtttttag
    15481 tccgtgttgc agcacggact ttttttacat tatataagaa agcgataagc gctttctgga
    15541 agtccgtaaa gattctttaa ttcctcaatt cgtttaggat attgatcatt gtcttctttg
    15601 tatagagaaa caatgagatt agcagcaaag caattagctt cgctttcaga tttgcttcta
    15661 gatgttcttg ttgatacata gtaactggat aagccacgat gaaaaatagc gtgacctaat
    15721 tcgtgagcgc aaatgtagaa tctttcctca gagtctctca gttcatcatt taagaagatt
    15781 attgcacgac ctctaatttc ttgaaactgt cctttgggat tctcgataaa aggaacgtat
    15841 tgaattttaa tgcccatctt ttcacaaata taaaaaggat tagcggactg gtatttccgc
    15901 ttcaactcct cgactaaatt aatcgtatcc atctccataa gctcacatct ttttgccttt
    15961 ttctttgtct tctttcacaa tatcccagaa ggttccaata aggatatctt ttacgcgttg
    16021 gacctgttca ggcgtcagtg tttctccgcc ataagacata ttgacatttg aatctagtaa
    16081 tttatcaagt tcaactactt catcttttgt tgcccagtca ggaacttggt ttctaccgag
    16141 taaataatca gtagttacat taaaataatc tgctacaagt tgtagtcttt tagtactagg
    16201 agttctattt ttccattgat aaatcgtgtt tttaggtata tttaattctt cttccaattg
    16261 agttatactt atgcctcttt tatgagctaa ttcctttatt ctatctagta aattcatttt
    16321 cgcacctcac aagctacgaa aacactaata aaaaaattag ttaaaggtgt tgacaactaa
    16381 taaaagtgtt agtatactgt tttcgtaagc taaaatatta gctaaaaaga cttgataact
    16441 aataaacact tcacggtcgg caaactctga aatgtaaatt actaggcatt tctgtgtctt
    16501 atttagctat gtctatatac taataaaaat attagttatt gtcaacgaat attagctaat
    16561 tttttagctt acagattatt tttttagaaa ggagctattt ttatgtctga gaatttagac
    16621 ttaaaaattc gagcggagat gagaaaaaga agaatgactt tcaaagaact agctgcgctt
    16681 atcggtattt caggagctta tttatcagat attctaaacg gcaatcgtga tggaaagaaa
    16741 gcacaacagc atatcgaaac agtgaaaaaa atattggaca tccgataggg gggtaggcga
    16801 tgacaaaact aaaaaaacaa gattttgtaa aaaaatacaa ttattctcca tctacttatc
    16861 aacgtcgaat gtcggaacta aaaaatacag caattttctc agcggcgtat gaacgggtca
    16921 caggacaaga agtttggatc aatacagaat tatacgataa atttttgtct ttcaaatcct
    16981 ataacaggtt acgcacaaga aaggtaacac ctaaagaatt tatcgagaag catttagttg
    17041 atttataaaa aacagaacat tttgagaggt gaaggttaat gggtaaattc aacagagcat
    17101 tggtattcag cgcaccgcta atcatctacg ctttaggact ttggggaagc aggcaagcgt
    17161 tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa gctgaagctg
    17221 agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa aagagaaact
    17281 agaatgtata ttactattac tcagtttaat tctttcacta atttctctat tgggaagttt
    17341 gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa tatcattttt
    17401 gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc aaggtaacaa
    17461 gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat tctattatca
    17521 tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca tctgcggttt
    17581 aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga atagttagaa
    17641 atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag ttcaacttct
    17701 agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa tataaaactt
    17761 attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa caccacc
//
LOCUS       DASJPC010000054        17734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJPC010000054 DASJPC010000000
VERSION     DASJPC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.054"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..209
                     /locus_tag="U4A13_001801"
     CDS             <1..209
                     /locus_tag="U4A13_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEN1780084.1"
                     /translation="KEIQPTELADIVKTTIRSSNGMISSLNEARNRHSLAHPNTNIIG
                     KREAKLIIGISSTVTDYISGYLDK"
     gene            276..770
                     /locus_tag="U4A13_001802"
     CDS             276..770
                     /locus_tag="U4A13_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1780085.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            826..1464
                     /locus_tag="U4A13_001803"
     CDS             826..1464
                     /locus_tag="U4A13_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780086.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1536..1931
                     /locus_tag="U4A13_001804"
     CDS             1536..1931
                     /locus_tag="U4A13_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780087.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1915..4368
                     /locus_tag="U4A13_001805"
     CDS             1915..4368
                     /locus_tag="U4A13_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1780088.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4365..6392
                     /locus_tag="U4A13_001806"
     CDS             4365..6392
                     /locus_tag="U4A13_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780089.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6389..7411
                     /locus_tag="U4A13_001807"
     CDS             6389..7411
                     /locus_tag="U4A13_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1780090.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7428..8357
                     /locus_tag="U4A13_001808"
     CDS             7428..8357
                     /locus_tag="U4A13_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780091.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            8602..8718
                     /locus_tag="U4A13_001809"
     CDS             8602..8718
                     /locus_tag="U4A13_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEN1780092.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8734..9537
                     /locus_tag="U4A13_001810"
     CDS             8734..9537
                     /locus_tag="U4A13_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1780093.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            9772..10008
                     /locus_tag="U4A13_001811"
     CDS             9772..10008
                     /locus_tag="U4A13_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780094.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            10015..11967
                     /locus_tag="U4A13_001812"
     CDS             10015..11967
                     /locus_tag="U4A13_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1780095.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(12039..12536)
                     /gene="dfrG"
                     /locus_tag="U4A13_001813"
     CDS             complement(12039..12536)
                     /gene="dfrG"
                     /locus_tag="U4A13_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1780096.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            12898..13938
                     /locus_tag="U4A13_001814"
     CDS             12898..13938
                     /locus_tag="U4A13_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1780097.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            14036..14224
                     /locus_tag="U4A13_001815"
     CDS             14036..14224
                     /locus_tag="U4A13_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1780098.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(14282..14635)
                     /locus_tag="U4A13_001816"
     CDS             complement(14282..14635)
                     /locus_tag="U4A13_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780099.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            15166..15588
                     /locus_tag="U4A13_001817"
     CDS             15166..15588
                     /locus_tag="U4A13_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1780100.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            15585..15815
                     /locus_tag="U4A13_001818"
     CDS             15585..15815
                     /locus_tag="U4A13_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780101.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            16313..16513
                     /locus_tag="U4A13_001819"
     CDS             16313..16513
                     /locus_tag="U4A13_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1780102.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            16540..17733
                     /locus_tag="U4A13_001820"
     CDS             16540..17733
                     /locus_tag="U4A13_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1780103.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         6095 a         2929 c         3377 g         5333 t
ORIGIN      
        1 aaaaagaaat tcaacctact gaacttgctg atatagtgaa gacaacgata aggtcatcaa
       61 atggaatgat tagctctctt aatgaagcaa gaaataggca ttcacttgca caccctaata
      121 caaatattat tggaaagaga gaagcaaaac taataatagg tatttcaagt acagttacag
      181 attatattag cggatacctt gataagtagc gtcattaact gattgctact ttttttatga
      241 ctatttttat aaaaaggatg tgataagaat tggaaatgaa agtctatgtg gcaaatttag
      301 gacggtataa tgaaggtgaa ttagtcggag catggtttac tccaccaatt gatgaggaag
      361 aaatggcaga aagaatcggt ttaaatgagg actacgaaga atatgcaata catgattttg
      421 agcttccttt tgatgtggac gaatacacgc cgattagtga aatcaaccgt ctatgtgaag
      481 ccattcaaga aatcgaaggt acacctattt ataatgaact caaagaaatt caaggcatgt
      541 ggtttagtag cttggaagaa ttactggaaa acaaagagga tattcattgt tatagtgatt
      601 gtgattcgat ggaagatgtc gcccgttatt acgtggaaga aacaggacag cttggtgaag
      661 tcccttccaa tttacaaaat tatattgact accaatcttt gggacgtgat atggaaatag
      721 agggtaatta cctagttacc tctcatgggg tatttgaata ttgtcagtaa gtttaacaat
      781 ccgtgttata attaaattaa taaatagcac ggagaggtat aaattatggt aaatgtattt
      841 gaaaatctta aactattaaa actagacatg aaagataaag gctgggtgat agattccttt
      901 tactttcgtt acaaacagca aaattatatt gtacttgtta agctatttga aaaagaggaa
      961 aaagttccag aatatgctct gttaaaacta gaatttttaa aggaaaacga tttttcagat
     1021 atgctagctg tctatgctaa ttcggtaaaa ttatatacag acacgaaaac aataagagaa
     1081 tattttggta tcgaatacag tagtaacttg ggagatgttt tatttcaatt tagccaaact
     1141 cttgctcgat ttattccgac agaagttagt gagaagaaaa atgaagacca aaaagaagcc
     1201 atgtgtttct cattaagtca aagtgattca gaagatccca gaaaaaaata ttgtttttca
     1261 gttagaagaa atcctttaaa gggaaatggt gagctgggaa aacgaagtcc ttttaatgac
     1321 aataaaacga gattgtatag acccagctta tatgaacgat tagggtcaga taccaattta
     1381 agtttcagat attcaatgaa tccagatgat gaagaaactg atgaaggtat tattgcaaaa
     1441 tggacaaaaa ataaaattga atagttaaag cattgaaaga gcaatcacct attttgattg
     1501 ttctttttct atttttacgg aaaggacgtg aagacatgaa aaaaataaga agttacacca
     1561 gtatttggtc agtagaaaaa gtgatttatg cgattaatga tttacaactc cctttcccaa
     1621 ttacctttac gcaaatgaca tggtttatcg tttctttgtt tgtcgtcata ctctttggca
     1681 gtttaccacc actttctctg attgacggag cactactcaa atacttaggg attcccattg
     1741 ttctcactgg gttcatgtgc caaaagacct ttgacggaaa gaaaccctat ggatttctaa
     1801 agtctatcat tacctatgct ctacgcccta aagtaaccta cgctggcaaa caaatacgat
     1861 taaaaaaaga aacaatgaat gaatcgatta cgattgtaag gagtgaacaa ctagatggca
     1921 tatccgatta aatatatgga agataatctc gtatttaatc atgatggcga agtattcgct
     1981 tattatgaat taattcccta taattacagt tttttaagtt ccgatgaaaa aattcaagtt
     2041 catgataatt tctgtcaatt gattgcccaa aatcgtgatg gcaagattca tgctctgcaa
     2101 ctagcaaccg aatcaagtat tcgagccaca caggaacaat cgaaaaaaga aatcactgga
     2161 cgattaaaag agatagctta cgaaaaaata gatgaacaga ccgaagcgtt aatttctatg
     2221 attggtgaca atcaagtaga ctatcgcttt tttattggtt ttaaactcta cctcaatgaa
     2281 caggaagtat ccgtaaaaaa tgttgggaaa gaaattcaaa aagcatttgc ggaattcttt
     2341 catggtgtga atcacaaatt aatgggcgat tttgtttcta tgcctatggc ggaaatcgaa
     2401 cgcttttcta agatggaaca gttgttagaa agtaaattat ccagacgctt tcagattcgt
     2461 aaattggaga aagatgattt tggttatatt attgaacatc tttacgggca aacaggtgtc
     2521 ccttttgatg actacgacta ccatttaccg aaacgatacg aagataaaaa agtgttggta
     2581 aagaaatacg atattctcaa acccactcgc tgtttaatcg aagaaaatca acgctattta
     2641 cggattgaac aggaagatac cactacttat gtggcgtatt ttacaattaa tagtattgtt
     2701 ggtgaattgg atttccctaa tagtgaaatt ttttattacc agcaacagca gtttactttc
     2761 cccgttgata ccagtatgaa tgttgaaatt gtcgccaata aaaaagcctt atctaccgtt
     2821 cgcaataaaa agaaagaact gaaagattta gatagccacg catgggaaag cgacaacgaa
     2881 accagtacta atgtggtgga cgctttagaa tctgtcgatg aattagaaag taatttagac
     2941 caaaccaaag aatctatgta caaactaagc tatgtggtac gggtatcagc aaagaatttg
     3001 gacgagctga aacaacgttg tgatgaagta aaagattttt atgatgatct gtctatcaaa
     3061 ttagtacgac cttttggcga tatgctaggc ttgcataatg aatttattcc ctcaagcaag
     3121 cgatatatca atgactatat tcaatatgtc acgtcggatt ttttggctgg tcttggtttc
     3181 ggtgctacac aaatgattgg tgaaaattct ggtatttacg tcggttacaa tctcgataca
     3241 ggcagaaata tttatctcca acctagcctt gcaagtcaag gaattaaagg ctctgtaaca
     3301 aatgcattag cgtccgcttt tattggttca ttaggtggcg gaaaatcatt ctctaacaat
     3361 atgctggtct attattcagt actctttggc ggacaggcaa ccattgttga cccaaaagct
     3421 gaacgtggtg aatggaaaga aaccttacct gatattgccc atgaaatcaa tattgttaat
     3481 ctcacgagtg agaacgacaa taaagggtta cttgaccctt atgtgatttt aaaactcccg
     3541 aaagattcag aaagccttgc cattgatatt ctcacgttct taacaggtat ttccagccga
     3601 gatagcgaga agtttcctgt cctgcgtaaa gctattcgga gtgtaactca aagcaaacaa
     3661 cgagggttgt tattggtgat tgatgaattg cgaaaagaag atacgcccat cagcaatagt
     3721 attgctgacc atatcgaaag ctttgtcgat tatgattttg cccatctgtt attcagtgat
     3781 ggcagtgtca cccaatccat tagcttggaa aaacaactca atattattca agtggcagat
     3841 ttggtcttac ccgattccaa tagctctttt gaagaataca ctaccatgga attattaagt
     3901 gtggcaatgc tgattgtcat tagtaccttt gctttagact ttatccactc tgaccgttcc
     3961 atttttaaaa ttgttgacct tgatgaggct tggagttttt tacaagtggc acaaggaaaa
     4021 gctctatcta tgcgattggt tcgagcagga cgtgccatga atgcaggggt ctatttcgtt
     4081 actcaaaacg cggacgattt actagatgaa aagatgaaga ataatattgg cttgaaattt
     4141 gcctttcgtt ccactgacct agtggaaatc aaaaagacct tagaattttt tggtgtggac
     4201 aaggaagatg aaaacaatca gaaacgctta cgtgaattag aaaatggaca atgtttagtt
     4261 tcagacatct atggtcgtgt aggggttatg cagtttcacc cgatttttga agagttgctt
     4321 catgcctttg ataccagacc gcctgtgcga aatgaggtgg aagaatgaca caaaaacaaa
     4381 agaaatttct tcactatagt tggataacgc tcctttgtgt gggcgttatt ttacttttat
     4441 taggcacgct gggcaatgca gtacaagcga caggcttagt tgatgaaacc attgatacaa
     4501 gcaatgaata ctccaagtat ggtttgaatc aatatcagct tgattattat gtggacaata
     4561 gctggggctg gcttccatgg aattggagtg atggcatagg acaatcggtg atgtatggat
     4621 tgtatgccat tactaatttt atttggacaa tttctcttta tctatcgaac gccacaggtt
     4681 atttggtgaa agaagcctat gccttagatt tcattagtca aacatcccaa gcaattggcg
     4741 aaaacatgca gacgattgca ggcattacac cgaatggctt ttccagttca gggttttatg
     4801 tcggtttcct ccttcttttt attctcattg ttggaatcta tgttgcctat acaggattgg
     4861 ttaagcgaga aacgacgaaa gtgatacgag ctgttttaaa ctttgtcgtg gtgtttattc
     4921 tatcggcttc ctttattgcg tatgcacccg attatattgc gaaaatcaat gatttttcca
     4981 aagatgtcag taccgccagt ctgaatattg ggacaaaaat tgttcttcct aacacggata
     5041 gtcaagggaa agacagtgtg gacatgatac gtgacagctt attctctgtt caagtaaagc
     5101 aaccatggct attgcttcaa tatggctcca ctgatgtaga agcgttaggg gaagaacgtg
     5161 tggaaagttt attgtctacc agtccagata caaacaatgg tgaagataga gaaaatgtag
     5221 tgattgaaga aattgaagat catgacaata cctatttaac cctaccgaag acgatttcca
     5281 gattaggaac agtcttcttt ttgtttgtat tcaatattgg tatctcgatt tttgttttcc
     5341 ttctaacagg aatcatgatt ttttcgcaag tgctatttat tattttcgct atgtttttgc
     5401 caatcagttt cctattaagt atgttgccga gttttgatgg catgggcagg catgccatta
     5461 ccaagttatt taatgtgatt atgacaaggg ctggtattac cttgattatt accgttgcct
     5521 ttagtatttc cactatgctt tattcgctgt cagcaagttc tccctttttc atgattatgt
     5581 ttttacaaat tgtgaccttt gcagggattt attttaaact gggcgattta atgagcctat
     5641 tcactttaca aagcaacgac tctcaaaaca tgggcagaca agtgtttcgc agacctcgtc
     5701 aaatgatgaa tcgacaaacc agaagatttc aacgcagtat gagtaagatt tttcgtggaa
     5761 aagcagggtc aacgaagcaa aagacaatga caacaaagca gaatcaaacc aataaagcta
     5821 accatactcg tcaaaatgaa cggactacac caaagaaaga aacagcactt tccaatgtga
     5881 aaaagcaaca aggcggtatc gggcaacgat taggtgaaaa gacagcccat gtattggaca
     5941 cgaaaaatcg tctagtcaat aaaagaaagc aggcaaaaca acagatacag gcgacaccaa
     6001 cccatgtcaa atatgccctt catcaaggaa aagagaatgt cactcataat gtaacagact
     6061 ttaaacaatc tatggcagat accaaagctc aacgcaaaca acagcgtatg aaccagcagg
     6121 agcaaaaacg aaagaacatt gctcaaaagc gtttggagct ggataaagca aaagaaaaaa
     6181 gacgaaaaga aaacccatca tctcctgttc agaataacca gcagcatgtt cgcaagcgtc
     6241 agcaaaccgt cacgcctgtt ttgtcacaaa acactaaacg tgaaaatcat cagcgaccat
     6301 tgaaaaaaga gcaaaaacaa aagacaccac cgaagcaatt caatcagagg aagctaaata
     6361 aaagaacgga taagcgagga caacaaacat gaagctaaaa ataacagtga tttgtagtgt
     6421 ggtcttctct ctcttctttt ttctcttatt attcctagtc attttctttg cagatgatac
     6481 ggacagtggc gaaaacaaca aggattcttc tattccacaa ggcggtgtga ccgtttcacc
     6541 agaagtgttg gcacaccgtc cgttgattga aaaatatggt aaggaatatg ggattgaaga
     6601 ctatgtttct tatatcttag ccattatgca agtggaatcg ggcggtacag cagaagatgt
     6661 tatgcaaagt tccgaatctt tgggtttacc gcccaacagt ttgagtaccg aagagtccat
     6721 caaacaaggg gttaaatatt tcagtgaatt attaaccagt gcagaacaac aaggagtgga
     6781 tatagattct gttatccaat cttataatta tggcggtggc tttttaaatt atgtgaggag
     6841 tcatgggaaa aaatatacct atgaattagc cgaacagttt tctaaagaga agtcgggtgg
     6901 tcaaaaagca gactatccca atcctatagc catacctgtg aatggtggtt ggcgatataa
     6961 ctatggaaat cagttttatg ttcagttggt ttcacaatac ctaaccgaca cttccccaac
     7021 agaatttgat gatgaaaccg tgcaagtcat tatggacgaa gccttgaaat acgaagggtt
     7081 tccctatgtc tttggagggg cgtcgcctac cacctctttt gattgtagtg gcttaataca
     7141 atgggtgtat gacaaagctg gcatttctct ccctcgtgtc gcccaagatc agtatgacgc
     7201 tacgcaagaa atctctatgg aagaagccca agcaggcgac cttatcttct ttcattcaac
     7261 ctataatgca ggcacatatg tgactcatgt ggcgatatat ttagagggaa atcgctttta
     7321 tcatgcaggc gacccaatcg gttatggcga cttaagcagt cgttattggc aagaccatct
     7381 gattggggct agacgtgtta ttcataacta agaaaggaat gaatcaaatg attataaaaa
     7441 tcgagcgaaa agaaaagaag gagaaaccaa agaaatctaa aaaaccgagc aagcaaagaa
     7501 aagttccgat gattaaagtt ggcacgcata agaaattaac ctttgtttta tgggttttat
     7561 tgattggtag tgtcggattt ggaatctata aaaattttac tgccattgac actcatactg
     7621 tccgtgaaac agaaatcatt aaacagcaga ttgtcgatac caaccaagta gaaagttttg
     7681 tgaaatcctt tgcgaaagac tacttttcgt ggcaacaatc ccaagaagcc attgataaac
     7741 gaaatgaaaa attgacccat tatttgacgg aagaattgca ggtattaaat gaagaaatga
     7801 ttcgtaagga tattcctact agttctagtg tgaacgatat acaggtgtgg caggtttctc
     7861 aagtgaatga gaacaccttt gaggtactgt tttctgttga acaagttatt accgaagaca
     7921 aagacaagga aactatatcg tctagctttc atgtggttgt ccatatagat gaatcagata
     7981 atatggttat tatcaaaaat ccgacaatga gtaagaaacc tcaaaaatcg gactaccaac
     8041 ccaaacagct agaaagtgac catacagtag atacagaaac gatggacgaa atcactagct
     8101 ttttagaaac gttcttccag ctctatccta ccgccacaga aaaagaattg acttactatg
     8161 taagtaatca tgtgttacct atgattaaca aggaatatgt tttcgaggaa ttggtgaatc
     8221 ctatctttac cagaaaagat aaccaagtaa tcgtgaatgt cgctgtgaaa tatttagatc
     8281 aagaaacaaa ggcaacccaa atctcgcaat ttgagttgat attagaaaaa caagataatt
     8341 ggaagattgt aaaataacaa atattggtac atgattacag atactttgta atcatgtact
     8401 ctttttgata aaaaattgga gattccttta caaatatgct cttacgtgct attatttaag
     8461 tgactattta aaaggagtta ataaatatgc ggcaaggtat tcttaaataa actgtcaatt
     8521 tgatagcggg aacaaataat tagatgtcct tttttaggag ggcttagttt tttgtaccca
     8581 gtttaagaat acctttatca tgtgattcta aagtatccag agaatatctg tatgctttgt
     8641 atacctatgg ttatgcataa aaatcccagt gataaaagta tttatcactg ggatttttat
     8701 gcccttttgg gtttttgaat ggaggaaaat cacatgaaaa ttattaatat tggagtttta
     8761 gctcatgttg atgcaggaaa aactacctta acagaaagct tattatataa cagtggagcg
     8821 attacagaat taggaagcgt ggacaaaggt acaacgagga cggataatac gcttttagaa
     8881 cgtcagagag gaattacaat tcagacagga ataacctctt ttcagtggga aaatacgaag
     8941 gtgaacatca tagacacgcc aggacatatg gatttcttag cagaagtata tcgttcatta
     9001 tcagttttag atggggcaat tctactgatt tctgcaaaag atggcgtaca agcacaaact
     9061 cgtatattat ttcatgcact taggaaaatg gggattccca caatcttttt tatcaataag
     9121 attgaccaaa atggaattga tttatcaacg gtttatcagg atattaaaga gaaactttct
     9181 gccgaaattg taatcaaaca gaaggtagaa ctgtatccta atatgtgtgt gacgaacttt
     9241 accgaatctg aacaatggga tacggtaata gagggaaacg atgacctttt agagaaatat
     9301 atgtccggta aatcattaga agcattggaa ctcgaacaag aggaaagcat aagatttcag
     9361 aattgttctc tgttccctct ttatcatgga agtgcaaaaa gtaatatagg gattgataac
     9421 cttatagaag ttattactaa taaattttat tcatcaacac atcgaggtcc gtctgaactt
     9481 tgcggaaatg ttttcaaaat tgaatataca aaaaaaagac aacgtcttgc atattaagta
     9541 gttcagtttt ggagtacaaa aagtgaaaat gtaaacctaa atgagtatca aattgcaact
     9601 gaatttgata ctcgtttttt attgttaaat caacgtttgt tgttaattta caccacaaaa
     9661 tgtaagttaa aatgtatata aaaatgcgaa cgtgaaataa ttattgtagg aatgcaaatt
     9721 ggtgtgttaa aatggttaca atttactaag caaaaagggg tgcattttcc tatgcaattt
     9781 cttgagccaa aaaataagaa cgctaagtct gtggattggg aaatatcaga gcaagtaagg
     9841 gtgatagtta agcaatacgc tgaatatgca gagcgtactg aaagcgaagc agtggatgaa
     9901 ttcttattga acattttaga cgataagaag tttattgaat ggattgccaa caagcgaagt
     9961 aataagcgga ttgttgaaaa aatgggtatc aaagatagag tgggttgata gacaatgacc
    10021 aagcttaaaa gattagccac gaaggaagat gaaattgtag atgttaaaat tccgatttca
    10081 aacgaagaat taaaagatag ggctaaacaa tatgatttat taactccaaa acgatttgca
    10141 acaaggtata acaaaatgtt gttcttgcca acgtctttta aatggaacgg ttctgaatat
    10201 cccattcaat ataattactg catcaatccg ttttgttgta attttgggaa agaacagcac
    10261 aaattcaaag atgtaaaagg gaaaccaagt cgatataaaa tgactggtag ttccaaagat
    10321 aaaggtcata aaggtatgta ctgcaatgac aatcctattg gcagaggtgt atcgcaaaac
    10381 tgtacagtaa cccctttatc taattggtct gttgttgaag aaattaaacg ccttatagaa
    10441 atcaatagca tccaagatgt tgagccagat tatcaatttc ataaagaagg ctgctcggaa
    10501 gaagaatcaa ccccattcaa tgagccgaag caattttaca aaagggggaa aagtaggggc
    10561 aaatctcaac gctatcaatg taaggcgtgt aagaagttca ctaatgttct accaaaaaga
    10621 gaagaaacca ctacttatca ccaacaaaag aacaccattc ttccaatgtt cgctaagatg
    10681 gttgttggaa gggtttcagt cagtcgtact tgtgatattt taggaattgg agttggaacc
    10741 tactatcata agctggagtg gctgtatagg cgttgtttgg agtttttaga acgatatgag
    10801 acacaacccc ttcaaacaaa gaaatttaac gagatgtggc ttaatacgga caagatgcac
    10861 tactacctaa ataacgtccg taagaaagga caaggttcaa agaaatatac ggggtttgaa
    10921 gaccttaata tgcaaacgta tatagttgta tctgctgagg tcttatcaag atatgtgttc
    10981 cgttctgatg ttgcctatga ttggaatatt tcaatggatg aattaaatga agatacaaga
    11041 aaattcaaag aagaccatct caatactttt agtcgtaaaa atgataggtt agattggtct
    11101 tattatccac aagaaccatc cgctaacgat agcgaaaata ggaatgctta tcttcacgaa
    11161 ttagggaaaa ttactaatag gagtagattt gtcgatggtt taaatgttga tgctccttat
    11221 actacaactg ctcactattg gttaattaaa caaatggtta atgccgacga atggcgaatg
    11281 attagtgatg atgacttctc tattagaaac gccttttata gagtattcac gaaagaatta
    11341 aggttgagcg atgcacatca ttttatttgc caagttaaca agacgaaaag cagaaaacaa
    11401 tgcttaaaag aattcggaca agcaaaagca gaactgctcg attggggaga tatcagagga
    11461 ttcaaaacaa agtttctacg gactttggca tctcattatc taactgaatt actaacaagt
    11521 catcaattcc acgaagaggc tattagtaaa gatggagaaa gatatcgcaa atatgccgat
    11581 aaccctatca aacatccatt agccactaag gataaaggat tttattcagt tgattgtaga
    11641 acggatttat ctgctctgga accaaacgaa atcgcaaaga tgctattaaa tgttaatgac
    11701 cattcgacca atagctttat tcaacagata agacgttata tatcatctct tgaaagacca
    11761 ttaacaacag ctcgtggtga taagaagagc tatatttacg ctaacttcaa tccgaagtat
    11821 gctcaatttg cgattacaat tctaagaact tactacaact tttgtcgccc ttttaaatca
    11881 gcggataaaa aggttttgac acctgctcaa agattaggga ttacagataa acaatttgat
    11941 tggaaagata taatttattt caaatgacaa aaagagcttg gagtaatccc aagctcttgt
    12001 gctttatctt tactgttctt cttcaattaa cgcacccgtt aactcaataa gttttttctt
    12061 tcatatacat gaaaatagta gttatacgga tttttatcat tctttatccc tttttgggca
    12121 aatacctcat tccattcctc ataattcact tctggaaaaa aagtatctcc ttcgaattca
    12181 tgatgtattt ttgtgatgta cattttctca acataaggga aaaacaaatt ataaatctgt
    12241 tctcctccga aaataaaaat ttcttcttcg tttttacata actcaaaaac atcttctatt
    12301 gaatgaacaa tttcacaacc attaaaggta aaccccttat ctctcgtcag aataatattt
    12361 cttctgtcag gtaaggctct tccgattgat tcaaggttct tcctacctaa tattatcgga
    12421 tgtccctttg tagtattttt aacatattcc cagtccttgg gaatcctcca aggaatgtca
    12481 ttctctttgc caatcactct attcttatcc atcgcagcaa tcaaagaaac tttcatttat
    12541 aacacagcct ttttaactaa tttaccatct aatttcttat ttctatcaaa aaaccttttc
    12601 tcattatcct gcccgttagt tgaataagca attttttcta taagttatat tatcataata
    12661 ccacaaaatc acaaattttc attcaattat cattttcaat tgcattttat aaatcatttt
    12721 gtatgtaatt tggtatgtaa gttaaaatga aaaaagtagt ccattttgaa gtacattttg
    12781 tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta tagtggagta
    12841 ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt tacagaaatg
    12901 tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg agaaattgtt
    12961 attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa actattgcca
    13021 cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga accgagtaaa
    13081 cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag tgatccgctt
    13141 ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt agggaaagta
    13201 caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat agaactaaaa
    13261 gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac cattcacatc
    13321 gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc gcttccgttg
    13381 ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca atcatttcaa
    13441 aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg ttggaatgtg
    13501 acggattgta aaatctgttt taagtacggt ttatactata gccctgttag tactccagca
    13561 gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc tggaacagaa
    13621 ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct ttcacgggca
    13681 tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa aaataatgag
    13741 gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa tgatttaact
    13801 ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca ggttaccact
    13861 ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt aagatatatg
    13921 ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc ttattaaata
    13981 agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga ttgttattat
    14041 aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac gaatacgagt
    14101 ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg aaactttaat
    14161 taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga cgcagagccg
    14221 ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg aattttactg
    14281 actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc attaaggata
    14341 gttctttgtc tgtaaagcta tccatgtatt tttctacctg taaccttcga gtgcttttaa
    14401 tcaaattatc tgtaggtaag aaaaattcat caatagatac atggagtaat gatacaagat
    14461 catacagcac ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt aaataacgtg
    14521 ggtcaatttc aatcattgtt cccacttgtt cacgagtgag accccgtttc attcgagcct
    14581 ctttaatcgc taaaccaacg gctctaaaat cgtatttatc ttctttttta cgcatactaa
    14641 atcacctcta tacattttac tgttcctata taaccataaa caggtataga aaaacatgtt
    14701 atatagttta tgagttccta tagaacactg tggaatttat taatgcgatg aatccaattt
    14761 caaatagaaa ggtcgctgat tttatttacc tatacaactt tataaaattt agtcatactc
    14821 ctagaaagta gataaaattg gcgacttaat ggagggaaaa gtcgccatga gaaagataat
    14881 ctgtcatcta gttctcttaa ttaagttctt atcaaaaaaa cttatccacc tgtactggat
    14941 agatatgacc atcaatgtgg ttttgcagat tacatagata gaaaaatcaa agcatatgaa
    15001 cagagaaaac aatctgttta tgtgcttttt tggtcactta ataaatatct tttaggattt
    15061 ctcttcattt atgcaacaaa tcccccgttc gtgtcggggt ttagtatgaa gggagataaa
    15121 gaagcacatg ccactccctt tcacgacaga aagggggtga aaagcatgaa accatcttct
    15181 tttcaagaaa ccatagaaaa tcagtttgac tatatctgca aacaagttat tgaagatgaa
    15241 cgaaaagatt acttcaaaca tctagacaga ctatcagcaa aagaagtctc gttttctgat
    15301 atagataact accttgtcaa ctctttttct actgttgata cgtacgtcac tgatttccaa
    15361 ctatttaaat tatttggatt aagaatcaat gttgaaagtg atttattaag tgaagccctg
    15421 cgtcatcttt cagagaaaaa acggaacatt attttacttc attactttat ggatatgagt
    15481 gatgtggaga ttgcggagtt actaaatttg aatcgttcga ctgtttatcg tcatcgaata
    15541 agtggactag ccatgattaa agaattcatg aaggagtgta aagaatgaaa acagcttatc
    15601 caagggttcc cttccccctc attgtaaagg cgacagatgg cgacgtggaa gcaattaatc
    15661 agattgttaa acattatcga gggtatacct caaaacgttc attacgtcgc atgacagacg
    15721 aatatggaaa ttctcatatg gttattgatg aaaccctacg tggcagaatg gaaacgaaac
    15781 tcatctctaa gattttatcg ttcgaaatta aatagtccca ttttttcgtg gaagcgtgtc
    15841 gaatcgtatc cacgcttccc gatttgggag gtttgttatt ccatcaaagc atattgtgtg
    15901 ttcagtaggt tttgataggc taacaagcca aacgttcttt gacaaccaaa taaaggcaat
    15961 cgaatacgtt tcggtaagaa aagagctgat gaactaacac gccatgacct acaaaattag
    16021 tgagcgattc atgttagtaa ttcgaaagta atctttagca ggattgctgc catgactttc
    16081 ttatcgtgat aatgatactc ccgtatagcc atagtccgag cgttagaagc gtcacaggca
    16141 atgggtacgg ctacatgaga accatgcaga agtgaaattc ctgtgagctt tgctagagct
    16201 gttcgattgc taagatattc ttctcttgtc agtgtattat tataaaacta tttgatataa
    16261 ttataggtga atattggagt tttcctagcc tataattggg aaaggagtaa taatgataac
    16321 ggtcacaaaa aaggatttag ttgagctagg atatggaact tctttttcag cagacatcat
    16381 aaaaaaagca aaagaattga tgattacaaa agggcatatc tactatcaat ctcgaaagct
    16441 agatagagtc cctcgtgaag ctgttgaaga aattctagga attcggtttt caaatgaaaa
    16501 caggtctgag tagtttttgc acagtaagga gtaaaaacta tggttaaaga cccaataaaa
    16561 aaagcaaaga atggaaccta ttattttcgt gcaaatctag gttatgattc taaagggaaa
    16621 aaaattcaaa aatatcgtag tggattcact actaaaaaag aagcaagaga agaatattcg
    16681 aaactgctat tgatgaaacc agaagaatta agtgagaata aggataaaat gacgtttaaa
    16741 cattatatct ttgaaatttt ccttccatgg tataagacac aggtaaagct cagaacatat
    16801 gaaaatcgct taccaactat taagaagcat ttctcttatt ttgataaaat ggctgtttcc
    16861 gatattgagc ctattgatgt gcaaaattgg caactaaaat tatcaaagaa gtgtaagtct
    16921 tcttatgtaa gagctgttca aggactattt tcagttgcta tggatcgagc aattgtcctt
    16981 gggataacca ccacaaatcc atctaagatt attggcaatg tgaaaaagca aaagtctaaa
    17041 attgaatttt ggacaaaaga agaatttgaa aaagtaatct ctctaattta caaagaagat
    17101 tactatcaac actttttatt tatctctctg tggtttttat ttatgactgg aatgcgaatt
    17161 ggtgaagcta ctgccattca atgggaagat attgattttg actcaggagt actaaccatc
    17221 aacaaaacac tctattacaa gaatcaatcg aattatagat ttgttgaacc aaaaacaaag
    17281 gcgagcgttc gtcatattgt attagataaa tgcacattaa cttatttatc cgaatggaag
    17341 gaagtacaac aaaatctaat tcagacagac tttgtaatga gctataacgg tattcctacc
    17401 caaaaacata cactatcata tgctattgaa cgctatgcta agctggctgg tatccatcga
    17461 atcagaatcc atgggttacg gcattctcat gcttccttgt taatcagtat gggagaaaat
    17521 ccacttatca ttaaagatcg tctaggacac gaagatattg agacgacttt aggaacgtat
    17581 ggacatcttt atcctaacag taatttcgag gtagctaaca aattaaatgg agccatttcc
    17641 tttaaagaag caaaacaaaa ttatgatact tcaccaaaga atcaatttac tgtagactac
    17701 ttgcgaaaca aagaaatgaa aaaagtgcaa taaa
//
LOCUS       DASJPC010000055        16322 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJPC010000055 DASJPC010000000
VERSION     DASJPC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.055"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..1819)
                     /gene="uidA"
                     /locus_tag="U4A13_001821"
     CDS             complement(11..1819)
                     /gene="uidA"
                     /locus_tag="U4A13_001821"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN1780104.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            complement(1847..3613)
                     /locus_tag="U4A13_001822"
     CDS             complement(1847..3613)
                     /locus_tag="U4A13_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN1780105.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            complement(3626..4420)
                     /locus_tag="U4A13_001823"
     CDS             complement(3626..4420)
                     /locus_tag="U4A13_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1780106.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(4431..5807)
                     /locus_tag="U4A13_001824"
     CDS             complement(4431..5807)
                     /locus_tag="U4A13_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1780107.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(5936..6583)
                     /locus_tag="U4A13_001825"
     CDS             complement(5936..6583)
                     /locus_tag="U4A13_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN1780108.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(6667..8094)
                     /gene="uxaC"
                     /locus_tag="U4A13_001826"
     CDS             complement(6667..8094)
                     /gene="uxaC"
                     /locus_tag="U4A13_001826"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN1780109.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(8168..9247)
                     /gene="uxuA"
                     /locus_tag="U4A13_001827"
     CDS             complement(8168..9247)
                     /gene="uxuA"
                     /locus_tag="U4A13_001827"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1780110.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9250..10881)
                     /locus_tag="U4A13_001828"
     CDS             complement(9250..10881)
                     /locus_tag="U4A13_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN1780111.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11198..11869
                     /locus_tag="U4A13_001829"
     CDS             11198..11869
                     /locus_tag="U4A13_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1780112.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(12705..13661)
                     /locus_tag="U4A13_001830"
     CDS             complement(12705..13661)
                     /locus_tag="U4A13_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780113.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(13770..14015)
                     /locus_tag="U4A13_001831"
                     /pseudo
     CDS             complement(13770..14015)
                     /locus_tag="U4A13_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14160..14582)
                     /locus_tag="U4A13_001832"
     CDS             complement(14160..14582)
                     /locus_tag="U4A13_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780114.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(14762..15133)
                     /locus_tag="U4A13_001833"
     CDS             complement(14762..15133)
                     /locus_tag="U4A13_001833"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1780115.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            complement(15467..15730)
                     /locus_tag="U4A13_001834"
     CDS             complement(15467..15730)
                     /locus_tag="U4A13_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780116.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         5150 a         3275 c         2221 g         5676 t
ORIGIN      
        1 aaaaggtaaa ttatttttta taactgaaat ttggtattga tgaccatcgt ttctttagat
       61 agtgagcaat cattttaggc tttctatctc tagtaaagat tcctttctta tttccttgca
      121 ctcttaaaat cccttgactt gtttcaaaat ctgcaaaatt ccacacttgt tcaccaacaa
      181 aattgtcaca tttatctatt acttcgtgat ttgcttcgta ataacccact tgatactctt
      241 ctgtaaacat tgttggtact gtatcatgaa gtccagctac tgtatctgct ccatattctg
      301 taaacattat tggcgtatcg ggacatcttt ccatccatcc atctagctct tttttcaaca
      361 gatattttgc atgttttagg tctccacctg aaacatacca accataatag cgatttaaac
      421 ataaaacatc gacaatatct cctactttac aaatatctgg tgttgacttt aagtaagtaa
      481 cgattgttac aggtctcttc tgagggtcaa gttctttagt caattcaact aaaggttcaa
      541 aatattcttt agcgccttca gaatccgaat ctggttcatt tgcaatagac cacattacga
      601 cactcggatg gttcttgtcg cgttctatca attccttaat cgccattttg tgagcatttt
      661 ctgtccgtat ttctttccaa gtatcatgtt tttctacgtt gtcatctaat aaagtagcca
      721 tgaaattcaa atgtaatcct actgcaggta cttcatcaat aactacaata ccttctcgat
      781 ctgctaagcg cataatttct tctgaataag gataatgtga agtcctaaat gaattagcac
      841 cgatccattt cattaaatta aaatctttga tatttactgc ttcattcaaa ccacgaccat
      901 taatatacgt gtcctcatgc ttcccaaatc ccttaaagta aaaaggttta ttattaatca
      961 aaaacttccc attcgaaacc tgaatactac gaatgccaaa ttcttctgtg tacgtatcaa
     1021 tcactttatt ttcgttaatt aaactaactt tcatactata taagtaagca tttagaggct
     1081 cccaaagtgt tgggttctta agttgaatca ctccttcttt ccccttagct tctgctataa
     1141 gttgagcttc ttcgtcatag attgctatta gaatttctcc ggtccattct gtatcgatat
     1201 taaattctac atttgcatct gaatcaatta tttttgtatt aatagtgaca tcctttatat
     1261 agtctttcgg agttgtatac aatttaacgg gtctattaat gcctgcataa ttgaaaaagt
     1321 caaaattaac caaattttct ttttttatgt ctccatcttc ctgtatcgtt tctttcaaaa
     1381 tccctacagg taaagtgcta tcgtcaataa cattgtttac agccactgta attctgttac
     1441 ttccatcttt tataaactga ttaatttctg attcaaatgg cgtaaaccct cctttgtgtt
     1501 gattaatcaa ttttccattc aaaaatactt ttgcttcatg tgttgcagat ccaaaacgaa
     1561 gaactaatct ttcttctaat aattttttag gaagaatcac ttctttttca taccacaccc
     1621 atccaacatg atttctaatg ttctctgttt caaataaatc attataagaa gaaggaactg
     1681 aaattggata actctctttt tcaaccattg gttctaaagc aagttcttct tgtaagtcat
     1741 catttgtatc taacttaaat ctccaaagac cgcttaaatc aattacttgc ctcgtctctg
     1801 tcataatggg atataacatg taaatcactc ctatataatt taaatattaa ataaatgtat
     1861 cctttaaccc acaataagca tctattggac ttattccctt aaattcagaa aggcccatta
     1921 gtttgtccac taatatttct aaagtttctt gttttccatc ataagtattg atataagttt
     1981 ttacttgagg cacatcagct aaatgaaacg gacactgtac agatacaaaa attgttggaa
     2041 tttcatgaat ataaaatgga atatccggag tacctttact tgctggccaa acaattcttt
     2101 gaactgtacc tgggttaaca ttagctatat taataatcaa gtcatatttt tcagttaaat
     2161 ctttgattgg tctcttagca gcatatattc cagctatatt tttaattttt tcttcttgcg
     2221 ataatccttt caattcttct tcaggtgaaa tgtatttttc aacttcaaat ccttttgctg
     2281 acaaaatctg tttcaataaa tcaatggcct gaggtctatt tccagcaact acttcaccaa
     2341 acccaccttt taccccatgt acatctacca gtaaaatcct tttatatttt tctggtacaa
     2401 taggccaaat atcttgttta tctttaacta gtgtaattgc ttgatcagct acctctttaa
     2461 aaattgattt attttcaatt aaaccaattt tttccatagc ttcttgtttc ggtaacaaaa
     2521 ttttattctt ttctttttta tgcaaaccta gtttagcctt taagcctaaa attcttctta
     2581 atgcatcacg taaacgctct tctgaaataa ttcctttgta ataaccttcc atcatataat
     2641 taaagtcttc atctggatca ttaaaaaata ggaatagatc acatccagcc gcaatagaag
     2701 tgggcaataa ttctgaccgt ttcattgccc cagtcattcc taacatatga ctagcatcag
     2761 taactactaa tccattaaac ccgagttttc ctctaagtaa gtcagtgatt aattcttttg
     2821 ataaagttgc aggaagtaaa tcttcatctt tcagatcaag attaaaatgt tttgaataag
     2881 ctggcatatg gatatgacct gccataattg aaggtaatcc agcttctatt aagcctgagt
     2941 atacttttcc aaaagaatta tcccattctt ctgtacttaa actattaata ctaaaagata
     3001 gatgttgatc tcgttcgtca ataccgtcac cagggaaatg tttagctgca ggcaaaattc
     3061 cactctcttg aatacctttc atataagcta aactaagttc taaaacttta tcagggcttt
     3121 gtccaaaaga tcgactagaa atgataggat ttctccaatt tctattaata tctacaatag
     3181 gagcaaaact ccagttacat ccaatagccg atgcttcaat tccagaaact ctccctaatt
     3241 cataagcata tttcgcatca tttgttgcac caatttttac ttctacacca acttcggtac
     3301 catctgtaca agctccgtta ccacctgctt ctgtattagc agcaattaat aaaggaattt
     3361 tgctttctgt ttgtaggata tagttttgat catatacctc atcagcagtt cctggattat
     3421 atcgtacagc tccaatatgg tactgattga ccatactact taagtaattt ttatcacgac
     3481 tagatcccat attaacaaat aattgaccta ttttttcttc taaagtcata gcatcaatcg
     3541 tctcttcaat ccaagaaata gcatcttcat ctaaatgata tggttttttt cgtaagtcta
     3601 cttttatagt cattttcttt ctcctctact tattagtaag atttttccaa aaagtatcta
     3661 tatatttttg ttcaatatct atatactctc ccgataaaca tttaaataaa atatcagttc
     3721 ttaagttttc ccatgattct ttttctttat taaccaaaat aggataaaag aaaactttat
     3781 tcttatttgc agcttctaga tcaccaggag aatcaccaat cattaatatc ttatccaaat
     3841 cacaaccatt ttcaattaca cttcgaataa tgtcttcttt ttttccttta tcttgacaac
     3901 acagttcatc aactaaggat aataattcat gtctttccca ttcttcttga atagcttcct
     3961 tattagctga gcttacaata attatcttcc catatttttt cagagctttt atagtatctc
     4021 tagcacttaa aaagggttta tctaatcctt tagcttgact aatctgtcga ttgacttctt
     4081 ccgaccattt taaagctttt aataaatcct ctgactgaga gtattctatt tccctcttca
     4141 aagatggatt agacaattct tttgttacag atacccagtg ctttaatcga ttaatcgatg
     4201 gtacatcatt tttttctaat gttttaacta aacctaaaaa tctgtttatt ccacgagtct
     4261 ctgaatataa attaatatta ttccaatcct gtaggaattt ttccctatga tgaacatcaa
     4321 aaatctctgc agcaattggc ccgaaaaaga gattgtgttt ataagtcatt gtatccatga
     4381 cacaaccatc tgaatcaata caaaatatcc aactgttcat gttttttctc ctagttatgt
     4441 ttttgattta gcaaattata attttctaat actttttctt tagataatgg atatacgaaa
     4501 aataaaatca atcctacaac aaagaaacaa actgctggaa tcaacgttga agagttatac
     4561 agcttttgta aaacttctcc actttgtact tttgctaacg aatcataccc tattatagaa
     4621 agagtccaac ctccgacacc gccagctaaa gcttgtccaa tttttcgggc aaaagaatat
     4681 actgcataaa tagttccatc ttctctttgg ccagatttca cttcttgatc atcgattaca
     4741 tcagtaatat tcgcccaaat gactgtatta aaaataccta accccatata tccaacagaa
     4801 gtaagaacta agaatacata catattacta ggctttatca aaaataaaag taaatataca
     4861 atagcagaga agaacatccc tactgacgcc aattcttttt taccgtattt tttactgaaa
     4921 attaaactta caggtgaaac aagtaccaac atcaaaatag gattgataaa gttcattaaa
     4981 actattccct gtgcattatt ataataatct ggaaatacat aattgttcat actcattgtt
     5041 aaaagctgag caagcaacaa aaaaatagct gcagctatta tagaaagcaa cgctctattt
     5101 ttagctaatc cttttaaaga tttcaacaat gaaactttta actgcgattc atcactttta
     5161 acccgttcta cagttaattt ataacatatt acgtaacaaa taactgcaca cactgaaaat
     5221 actcctgcca caattgtaaa agttgatcca ccacgtacaa tttgagctcc agaactatct
     5281 cttgtataaa taaaaattgg tgttaacacg ccaatgaaca tacctgctaa ggtagctcct
     5341 atactacgaa atgttgataa cgctgtcctt tcttctgcaa cagaagtgat agctgatgcc
     5401 attgaaccgt aaggaatatt aatagatgta taaaaaatac ttccccatag tatatatgtt
     5461 acatacatat atataacttt aatactcata gaagctgatg ccattgatgt ttgatacatt
     5521 aaaaaacttg ctaaagcgac tggaccactc atccgtaaaa tccacggttt aaatttgcca
     5581 gttttagatg gttttgatcc atccacaata cgccccattg ttatgtctgt aactgcatca
     5641 ataaatcttg ctactaaaaa taatgttcct acggtagctc cgcttatccc caatactttt
     5701 gtataaaaaa tcattaaaaa agaactagca aaaatgaaag tgaaatcatt cccaaaatct
     5761 ccaaacatat atcctatttt gtctcttaga ccaaattttc tttccatata atttgcgctc
     5821 cttcctattg ttaagcgttt tcattctgtt gaactgccaa cttacgtact atactctgaa
     5881 gtataaagag ttagtattta ttttttacat tttatagtat tatttatttt ttctcctaat
     5941 aattctttat ctcataaaac ttatctatat atttactagc tattcgagtt acttcttcaa
     6001 aatcaccttt ttcaacagga gccaacaaat tcccacctac accaactgca acaacgcctg
     6061 cttcaaacca ctcttctaag ttatctagac ttactccgcc agtaggcatt atatttacat
     6121 atggtaatgg agcttgatac gtttttacaa cagatggacc aaaagcagaa cctggaaata
     6181 gtttaatgat ttctactcca gcggtaagtg cctcctttat ctcagtaatt gtcatacaac
     6241 ctggtaaata aggaatttga tataaattac aaatctctgc tgtttgtcga tcaaaagatg
     6301 gacttacgat atattctgct ccagccataa ttgcaatacg agcagtaaac gcatctaaaa
     6361 ctgtaccggc tccaattatt acatcttttt gatcagcata ttttttagca atttctttga
     6421 ttatttcttc agcattcgga actgtaaaag ttagttcaat ccctttaatt ccacccgcag
     6481 taattgcatt tattgcttta atggcctcat ctttattttt tccacgtaca accgcaatga
     6541 ctccagccgc ttctaactta cttaaaattt ctactttttt catatctatc tccttctttt
     6601 atggatacat actctagcta gttgtactaa cttacaataa ataaatttaa gcttttattt
     6661 tttgtattat tttctacttt cgatttcaaa aaaattaaaa taattatacg cattacgata
     6721 acaaatattc tccactatct gactaagata catttcatca tctggaattc gacctgcttt
     6781 aacccactcg cttagtaatt gacataatac tcgacggaaa tactcatggc gaggatagct
     6841 tagaaaactt cgagaatcag tcaacatacc tgtaaaattc gacaacaaac tttgttgcgc
     6901 catcgtagtc aactgttgac gcattccttc agctgtatca ttgaaccacc aagcacatcc
     6961 caattggatt ttttgaatca tacccccttg gaagctttgc attgctgttg ctagtggaag
     7021 ccaatcggtt ggatttaatg aatataaaat tgtttttggt aagcacccac tcttttcagc
     7081 agtgtcgaac aatgctacaa cattttgtgt caaactagct tgatccccca tcgaatcacc
     7141 accgacatct actccaattt gttcctttaa tatacttgaa ttatttctta aagcatttaa
     7201 atgcatttgc atcgtccaac catatcttgt atactcagac atcaaatgta attgaatacc
     7261 tgtaacaaat tgtgcaattt ctgataaatg gaaatccgtt tttcctgcta aagcttttat
     7321 taacagattg tcgagtattt tttcatctgc tatttcataa aaataagtat tcaaaccgtg
     7381 atctgctaat cgacagccaa tttgatgaaa ataatccact cgttggctca atgcctgctt
     7441 caacccatta aagtttttga ttggtatacc cgctgcttga ctaagtttta aaatatagtt
     7501 cgaaaatcct gatgatttga tactccagac cttatctgga cggaaagtag gtaatacctt
     7561 gaaagaatta tcctgtttag aaagctcagt atgatatcgc aagtcatctg taggatcatc
     7621 cgttgtacaa atggctttca catccatcat cttcagcaat tcttttgggc gataggaatc
     7681 cgtttggatt ttttcgttgg cacgtcgcca aatttctggt gcattcttct cagaaattgt
     7741 caaatcaata tcaaagtaac gttgtaattc caaatgagac cattcaaaaa taggattacc
     7801 tggcgccttc tccactgttt ttacaaaagc taaaaatttt tcataatcat tacctgcacc
     7861 actgatcaac tcttcttcta tcccattagc tcgcatcaat cgccatttat aatgatctcc
     7921 agctccttta tcatttaacc atacctgagt caaattcttg tattgcttat tttctagcaa
     7981 acatgctggg tctaaatgac aatgataatc gatgattggt tggtgttctg caaatttatg
     8041 aaaaagtctt ttagctattt cattctctaa taaaaagttc ttatctaaaa acatatttaa
     8101 gcactccttt ccttccctta gtttttagaa gagaaaattt ttcttctaaa tagactccat
     8161 tatataatta tttactcgcc cccttttcaa gcgcttccca aataccattt aaataaactg
     8221 cacctagagc tcggtcatat aacccataac caggtctacc ggtttctccc caaatcatcc
     8281 gaccatgatc tggacgaata tagccattaa atccattatc gtacattgtt tttaatatct
     8341 tatacatatc tagagaacca tattctgatt tatgcggcgc ttcataaaaa tcgagtccat
     8401 tatcacttaa atatttgata ttacgaacat gtgcaaaagg aatccgatcc cgttgacaaa
     8461 attcagatag aatttcatag acatcattat ctggtcgttc tgcaatactt ccagtacata
     8521 acgtaatacc attagaagga gaatccacta cattacacaa ccattctaaa tcgtcacgat
     8581 ttttaacaat acgaggtaat ccgaaaatag aatatggtgg atcatctgga tgaactgcca
     8641 ttttaatccc aacttcttca catgtcggga taattgcttt gagaaaatat gcaaagttct
     8701 ctcgcaattt cgcttcatca acttctttat aggcttctaa taattgcttg atttgtgata
     8761 accgatgagg ttcccaaccc ggcaattcaa aattattagt agaattttct acttttctaa
     8821 taatttcctc tggatcagta ggaatatttt ttgaaatata ggctaacgta gaagacccat
     8881 catctaaatg ataatccaaa tctgatttta cccaatcaaa gactggcata aaattataac
     8941 agataacctc aatcccaaat tctgccaaat tgcgaatcgt ctgtttataa ttctcgatgt
     9001 attgatctcg tgttggcaaa ccaattttga tatcatcatg aacattaaca ctctcaataa
     9061 ctttcaaggt aaggccattg gcttcgactt catcttttaa agctttaatt cgttctctcg
     9121 gccagacctc tccggctggt atatccatta atgttccaac aattcctttc attgctggga
     9181 tctgacgaat ttcttgtagt gagatttttt cttcatttgc cccataccat ctaaaagtca
     9241 tttccatagt tactttcctc caacttctgt tatatattta ttgattgttt tacgaactgc
     9301 ttgaggacct actagcattt cgcaaaagat tttttcaatt ttctttccta tctcaacttg
     9361 atataaatct aaaccaaata tttccttatt ttctaagatt tcctgtaccg cacaatgaat
     9421 ctttgcagga tcttgttcac ctaatttcaa tactgagaat ttttcctgca actctaccaa
     9481 taaaggatca ggacttggca caaaattttt tccttgatca tcgactgcca ataaataacg
     9541 taaccaagca gcaatagcga taggaataaa tgttagattt tctgcatttc cgtctttttc
     9601 gacatacttt ttaattgttt caccatagcg aatcgctact ttttgagaag tatctgttgc
     9661 aattcgttgc ggtgtatctg gaatataggg attaggcaaa cgtttattga ttacttcatc
     9721 aataaattgt tttggatcaa taacgcctgg actttcaact actggtaagc cttctacata
     9781 acctatccct ttaattaagc caaccaaatc atcatcttgc atttctgcct gaattgattt
     9841 ataccccaac aaacaacctg ttattgctaa tgccgtatgg agcggattta aacatgtcgt
     9901 aaccttcatg atatctgctt tatcaactgt ttctctatct gtcataatca caccagccgc
     9961 ttccagttta ggtcgaccat ttggaaaact atcttcaatg actagataat ggactttttc
    10021 agtattggta aaagcagcga tattggtatt tttcgcagta tgaattatat ctaagtcttc
    10081 gaaacccgcc ttcaataaat ccattttgac actttccgaa ggatttggcg taattctatc
    10141 aatcatactc caaggaaaag tcactttttc tttatccttt aaataagcaa taaatttttc
    10201 agaaacgtat cctttttttt gccaagcttc tgcaatcata aaaatgctat cttgaaaacg
    10261 ttgaccattc tgtgagaaat tatcagtaga aaccatcgca atcggtaact ctccagcatt
    10321 aaacctgcta tacaacaagg atgtcacaat actcatcgta tgttgaggat tggtaggtcc
    10381 agattttata tctgcttcca ctaccgaaaa aaattgtcct ttagaatctt ttaaactgta
    10441 tcctttttca gtaatagtaa acgttgtaaa ctgtaatgta ggatcttcaa aatatttaac
    10501 cactctttga tagtccgtta tattctttgg attgcaatat aaactatcag ctgttactgc
    10561 taaaagtctt tttttgagtt caccatcttc acccataaca atctccaaaa tatcattgtt
    10621 gtagtcatga taagcttttt ctactacttc ttcatcaaat gtctcacaca caactactcc
    10681 ggattctaat tcattttgat ctgctaagca ttgagcaatt tctgcatgaa atcctcgata
    10741 aagatttcct ccaccaaaat gaagccacac tggatgtttc tgaccttctt gtttgaccgc
    10801 ttcgacatta aaatacggta gttgaacttg cattttgttc aatccttcga tagaatcttg
    10861 ccagtcttta atcaaaacca tatttacccc tccatactga tatgttagtt tccgttacaa
    10921 ttgtagtata aaatcgaatg taatcgctgt caataagaaa aaactaaaaa tattcttttt
    10981 tctaccatgt cagaattatt gtgtcttttt ttagtaaaag cacaccaata tatatttttt
    11041 agaactaact taaattaaag cttaatttct ctttgatctt atttgagtat tttttgtttt
    11101 tttaatagct agtatgttac tttgaaattt gatattatat aacgagcata gaactaaaat
    11161 atcaaattca aaaatgaaat tttgagaggg attaaatatg acaattaaaa ctagtctcca
    11221 aaatcaagct tatcaatcta ttcgtcagca aattatctac gctgacttag aacctggaaa
    11281 aaaagtatca gaaaaaaaat tggaagaaac gctttctatc ggtcgtactc caatacgtga
    11341 agcattgatt caactccgtc atcaaggact tgttgataca attcctcaaa gtggaaatta
    11401 tatctcatta atcgacttag atttagcaaa aaatgctcgt tttgtcagag aacatttaga
    11461 acgacaaatt atgctagagt gttgtgccaa gatgaataat caaaagaaac aagttttaga
    11521 aactataatt gcagaacaag aaaaagctgt tagtcaaaag aatgctcgta gtttttttca
    11581 taatgataat ttatttcata aagcttgttt tgaaattgca gaacgggaag aagtatggca
    11641 atggttggaa gataataata cgcacttgga acgatttcgt tggttacgag taaaaacgga
    11701 aggattaaaa tgggaaaaaa ttatggaaca acatcatcaa ctatttaaag ccttgaccat
    11761 gcaagaccct gaagaagcta acttcttaac atccttgcat cttcatttaa tgcttgatga
    11821 acaaaatgtt gtattgacta ctcacccaaa atattttaaa aatatataac tgctaaattt
    11881 ctttttttta atttatttac atttctgatt actacagaaa actaactact ttttttatgt
    11941 aaagttctgt caaatatagt cactttatat tactttctct tgagtaaaaa aacatgtgag
    12001 gtcgtaaaca aagattgaac tatctacatt catactctat aatctgttta tttgcagact
    12061 gataatagca aaagaaaact tataatcaaa taatatcaac atttgatcac ataatttgag
    12121 attgaatgct gatttaccta tgttttacgt aatggtcttt agttattttt gctcataaaa
    12181 tgcataaaac acgcgttttt atgtgtgctt gaaatttctt ggctctataa tttctaagac
    12241 tgataaaaat attctatcgg ttctttttgc aaataaaaaa acatatagaa gctccattag
    12301 attgaatcac cacaacacaa tcaaaggagc attccgtatg cttgattttc attctataaa
    12361 actactttta ggaatcaaag aaaatcacaa cggttcaaac taaaagcaag aatatccgaa
    12421 gtaccattgt ttcatcgatc atgagaaaag cattcacctc tgtctgcaga tgttcttata
    12481 tcgctctgtc agtcctatta ataaagtact gttttgtttt caaaaaaact attacgagta
    12541 ctttttaaga gaatgccttg caaatattca tcacacaccg ttatataagt caaatagttt
    12601 ctttcagtcg tcaagaaaag attctcctgt ccttggcatt atcagttaag tagcaactta
    12661 attctcggac ttgttctact ctagcaaaat ttattttcat caacttacgt ccaaatgttt
    12721 ttttgaataa ttacttcatc gcgggtctgg aagacttgcc tcattcgata agtcactagc
    12781 ttttggcgtc cttcaggatt tctagtatac accaactgac atttgggcag cgacgattga
    12841 tttaattcgt ttagaaaaag gttagcgatt tgcttatttc ggccaaagcg ttctggtacc
    12901 gtcagaacta tctttttccc ttcgattaaa taccgcggat tctccagcgg tgctaaaagc
    12961 tccctcatag cttcagtaaa aagctgtcct tctcgatagc cagcattctc aagatagcat
    13021 ctaaattcct gctgatcagc cgctacgatt acttttctat cactaacttc accaaggcgc
    13081 tgcaacgtcg tttgaacgca tatggccaag ctttttttgc gggcacattc cttttcccat
    13141 agcttttgtc gcctacgaat ttggaaatat tccagcaagt tacctttaaa tatagctgac
    13201 cagaagctta gaggctctat attttttact actatcggca atgaagtttc ttgtttttgc
    13261 tttacctgat cgactttcct aacgacgatt ggcatagata ggtgccagcc atcagctaaa
    13321 gcagtactcc attgttcttt caaccgactt cgttcacaag aaaaatagaa catcgactga
    13381 gtataagttt ccatcgtggt ggtcgtatat tccagtggta aagcaaagcg atcgccacca
    13441 ctctcaattt ttggtgaaga ttcaaaggtc agacctgaaa tattgcccaa acgccgtagc
    13501 attcttttca aatcaggctg ttcagacaga ggaactcccg gtaatccaat cgctaaatgc
    13561 caaatgttcg ttactttatt ttgtgcacct aggcgtaagc cccgccctcg tagttgttgt
    13621 gtttgcacaa aagtggaagc ttgattagcc aagatcaaca gattaattgc aggacaatcc
    13681 cagccttctc ctagcaaact aacggttcct accaaaatat tcaacttctt ttcggttaat
    13741 aggcgagtaa ctgcttgcat caatgcttgg cggtttttgt catccaccgt caaataaaaa
    13801 taacccgaca tataaggatg ttcattcata cccgtcgcta gttctgccag ctcaacagtt
    13861 actagcattt tcggtaacag taagaactct ccacagatgg caccgatttg ctgagtgtta
    13921 cctaatagac gaacaacttc atcaaacaaa ggaaccacgc cataagctaa ggtaccatca
    13981 ctgcctgcta atacctcctt gcaaatacga tccatcaaaa tgaccgctgt cagttcttgc
    14041 ttcagctgaa cttcttcttt taacaaaatt tgacaaactg cttgcttttt ctcaggaatc
    14101 gcctgactac caacagtgtc tggatactct ggaaacaaat gcagcgactc atcaaacaat
    14161 catccagctt ttttcaattg ataaaaaata ggcaattccg gttgatcttc aaacatctgc
    14221 tgataaatag tgatcagctc ttttttacct aaagatggaa ctgaagcttt tttaccttgc
    14281 tgcttagaac tgcctaatag ctgccaatgt tcgggtatca attcatagtt atgatgattc
    14341 aagtaaatta atgcactagt gaacagctca aaattttcat aaatttcctt cgaatgctca
    14401 agcggatgct gaacaaattc taaagaaagc aaataatcca ccgtctcttg gctttccatt
    14461 aacgcttcca ccagctcatt ttgtttctct aaaaaggtaa aataatgctg agtagtttcc
    14521 tgattagccg gaatcaaata tagaaaatcc tgatgaggac atagaacctc ctcctttacc
    14581 aactcgggaa tgctaatttc ttcgtcaatc aggccatttt aactcaatat attgcttcca
    14641 ctccgtctga gagctatcaa tcggaggcgt ggctgtcaag gaaatcgtca acagattcgg
    14701 ttgttttttc ttcaagtcaa tcaacaattc accccagctt ttttttaaat gatgagcttc
    14761 atcaaaaaac taacagctcc agctgatcac cataagttgg ctcctccttc agtcggttgt
    14821 aaatggtctg atacgtttcc actgtaatat gactaggatg ttcaatcgcc ccagaaagat
    14881 agcaactatc tgctggattt actaagaaat attcctcgat tgttgttaac cactgtttct
    14941 tcaatattag ggaaggaacc aaaattaagg ttttacaatc caaacgtcgg atcatctcaa
    15001 ttcctaaaaa ggtcttgcca gcccctggtg gagccactag atggactgaa ttgttattta
    15061 aataagtagc aaagttagtc aatatacgct tctgatagct acgccactga attttttcat
    15121 cagatagcca catcccaggt ccccctctca cgtattttct ctatcataat ctaaatgaat
    15181 acagcaaaaa aaagatttct tcaagaatat gtgtttttat agtaatattt tacttactct
    15241 aaactcaaat gaaaaggtaa aaaaactggt agtacaatct tttaaattct cattgttttg
    15301 tacgttcttt actatcaaaa aaacaaaaaa aacgcgtttc tacgcgtgac caaaattttt
    15361 agctctataa tttctaagac tgatagaaaa tcattctgtt tttgaagtct tttttcataa
    15421 atactacttg atttattgtg tatattgtat aaaagattgt tcataattat tttttataat
    15481 taaaatttgg tattgatgac cagcgattcc gtatataatg cgcaatcaaa ttaagctttc
    15541 tgtctctggt aaatattcct gttctgtttt cttgtactcg taagatttct tcacttatcg
    15601 caaaattcat aaaattccac actttttcat aaacaaaatg tacacattta tctattactt
    15661 cataatagtc tacttgatat tcttctgtaa acatggttgc tactgtatct gcaccatatt
    15721 tgataaacat ttgggctact cccttaaatt gagaaaagcc cattagttta tctactaata
    15781 cttctaaaat ttcttgcttt cttctttaac atcgtcccgt aaggaatcat gtagtatgct
    15841 ggaggaagtt cattttctaa ataattgtat cgcttcaatt gctcaaaggc tattctctac
    15901 aattaattac accaattact ttatcttcac ttatataaga tttctaaaca tttttcgtca
    15961 aatgaagaga aaatattttt ttaaatgatc ttgctcaaga tttgtcaacg aatctttgaa
    16021 ttcgttttga agttctgagg aaggtatcat ttaaaaactc aatctgactt tcatcatttc
    16081 gtttactccc attttgatca ccttctacca acaatttatt aaagtgatta tactaggttt
    16141 ttattttttt aattcacaat tgtttattca cttcatgaac aattaaacac aaaatcagta
    16201 ttaggattaa actttcaccc attaatacac taacgaaaaa attttcattt tttaaaatag
    16261 ctaaactata aatctctata ctgaaaaaaa caatacccat caaaaatact gggataactt
    16321 tt
//
LOCUS       DASJPC010000056        16141 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJPC010000056 DASJPC010000000
VERSION     DASJPC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.056"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(296..1024)
                     /locus_tag="U4A13_001835"
     CDS             complement(296..1024)
                     /locus_tag="U4A13_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEN1780117.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="U4A13_001836"
     CDS             complement(1002..2576)
                     /locus_tag="U4A13_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN1780118.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="U4A13_001837"
     CDS             2805..3743
                     /locus_tag="U4A13_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1780119.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="U4A13_001838"
     CDS             3755..4666
                     /locus_tag="U4A13_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1780120.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="U4A13_001839"
     CDS             4690..6162
                     /locus_tag="U4A13_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN1780121.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="U4A13_001840"
     CDS             6213..6824
                     /locus_tag="U4A13_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1780122.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="U4A13_001841"
     CDS             6854..8767
                     /locus_tag="U4A13_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEN1780123.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="U4A13_001842"
     CDS             8895..11876
                     /locus_tag="U4A13_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEN1780124.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="U4A13_001843"
     CDS             11873..13534
                     /locus_tag="U4A13_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEN1780125.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="U4A13_001844"
     CDS             13574..14506
                     /locus_tag="U4A13_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEN1780126.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..>16141)
                     /locus_tag="U4A13_001845"
     CDS             complement(14898..>16141)
                     /locus_tag="U4A13_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN1780127.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT         5188 a         2618 c         3218 g         5117 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 g
//
LOCUS       DASJPC010000057        16078 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJPC010000057 DASJPC010000000
VERSION     DASJPC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.057"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            164..1309
                     /locus_tag="U4A13_001846"
     CDS             164..1309
                     /locus_tag="U4A13_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1780128.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="U4A13_001847"
     CDS             1446..1790
                     /locus_tag="U4A13_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN1780129.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="U4A13_001848"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="U4A13_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN1780130.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="U4A13_001849"
     CDS             complement(3485..4084)
                     /locus_tag="U4A13_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN1780131.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="U4A13_001850"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="U4A13_001850"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN1780132.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="U4A13_001851"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="U4A13_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN1780133.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="U4A13_001852"
     CDS             7290..8408
                     /locus_tag="U4A13_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN1780134.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="U4A13_001853"
     CDS             complement(8468..9760)
                     /locus_tag="U4A13_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1780135.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="U4A13_001854"
     CDS             9892..10470
                     /locus_tag="U4A13_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1780136.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="U4A13_001855"
     CDS             10599..10976
                     /locus_tag="U4A13_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN1780137.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="U4A13_001856"
     CDS             10909..12981
                     /locus_tag="U4A13_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN1780138.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="U4A13_001857"
     CDS             complement(13192..14139)
                     /locus_tag="U4A13_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1780139.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="U4A13_001858"
     CDS             14385..14849
                     /locus_tag="U4A13_001858"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN1780140.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="U4A13_001859"
     CDS             complement(14911..15954)
                     /locus_tag="U4A13_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN1780141.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         5255 a         2931 c         3388 g         4504 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaata
//
LOCUS       DASJPC010000058        15982 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJPC010000058 DASJPC010000000
VERSION     DASJPC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.058"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="U4A13_001860"
     CDS             226..894
                     /locus_tag="U4A13_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN1780142.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="U4A13_001861"
     CDS             complement(1094..1480)
                     /locus_tag="U4A13_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780143.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="U4A13_001862"
     CDS             complement(1452..2834)
                     /locus_tag="U4A13_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN1780144.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="U4A13_001863"
     CDS             complement(2990..4177)
                     /locus_tag="U4A13_001863"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN1780145.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="U4A13_001864"
     CDS             complement(4184..5215)
                     /locus_tag="U4A13_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN1780146.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="U4A13_001865"
     CDS             complement(5231..6685)
                     /locus_tag="U4A13_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1780147.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="U4A13_001866"
     CDS             6860..7765
                     /locus_tag="U4A13_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN1780148.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="U4A13_001867"
     CDS             7856..8533
                     /locus_tag="U4A13_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1780149.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="U4A13_001868"
     CDS             8530..8700
                     /locus_tag="U4A13_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780150.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="U4A13_001869"
     CDS             8814..9710
                     /locus_tag="U4A13_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1780151.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="U4A13_001870"
     CDS             complement(9765..11135)
                     /locus_tag="U4A13_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1780152.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="U4A13_001871"
     CDS             11373..12119
                     /locus_tag="U4A13_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1780153.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="U4A13_001872"
     CDS             12116..12955
                     /locus_tag="U4A13_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1780154.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="U4A13_001873"
     CDS             12956..13906
                     /locus_tag="U4A13_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1780155.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="U4A13_001874"
     CDS             14047..14799
                     /locus_tag="U4A13_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780156.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="U4A13_001875"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="U4A13_001875"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN1780157.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5187 a         3001 c         3220 g         4574 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta ac
//
LOCUS       DASJPC010000059        15871 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJPC010000059 DASJPC010000000
VERSION     DASJPC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.059"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(307..729)
                     /locus_tag="U4A13_001876"
     CDS             complement(307..729)
                     /locus_tag="U4A13_001876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN1780158.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(743..2149)
                     /gene="atpD"
                     /locus_tag="U4A13_001877"
     CDS             complement(743..2149)
                     /gene="atpD"
                     /locus_tag="U4A13_001877"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN1780159.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2172..3074)
                     /locus_tag="U4A13_001878"
     CDS             complement(2172..3074)
                     /locus_tag="U4A13_001878"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN1780160.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3088..4644)
                     /locus_tag="U4A13_001879"
     CDS             complement(3088..4644)
                     /locus_tag="U4A13_001879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN1780161.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4667..5209)
                     /locus_tag="U4A13_001880"
     CDS             complement(4667..5209)
                     /locus_tag="U4A13_001880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN1780162.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5196..5720)
                     /gene="atpF"
                     /locus_tag="U4A13_001881"
     CDS             complement(5196..5720)
                     /gene="atpF"
                     /locus_tag="U4A13_001881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN1780163.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5811..6026)
                     /gene="atpE"
                     /locus_tag="U4A13_001882"
     CDS             complement(5811..6026)
                     /gene="atpE"
                     /locus_tag="U4A13_001882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN1780164.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6079..6798)
                     /gene="atpB"
                     /locus_tag="U4A13_001883"
     CDS             complement(6079..6798)
                     /gene="atpB"
                     /locus_tag="U4A13_001883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN1780165.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7100..9943)
                     /gene="rnr"
                     /locus_tag="U4A13_001884"
                     /pseudo
     CDS             complement(7100..9943)
                     /gene="rnr"
                     /locus_tag="U4A13_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(9946..10701)
                     /locus_tag="U4A13_001885"
     CDS             complement(9946..10701)
                     /locus_tag="U4A13_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1780166.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10804..11040)
                     /gene="secG"
                     /locus_tag="U4A13_001886"
     CDS             complement(10804..11040)
                     /gene="secG"
                     /locus_tag="U4A13_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN1780167.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11158..11436)
                     /locus_tag="U4A13_001887"
     CDS             complement(11158..11436)
                     /locus_tag="U4A13_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN1780168.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11549..12517)
                     /locus_tag="U4A13_001888"
     CDS             complement(11549..12517)
                     /locus_tag="U4A13_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1780169.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12732..12776
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12800..13774
                     /locus_tag="U4A13_001889"
     CDS             12800..13774
                     /locus_tag="U4A13_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEN1780170.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13779..14354
                     /locus_tag="U4A13_001890"
     CDS             13779..14354
                     /locus_tag="U4A13_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1780171.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14421..15560)
                     /locus_tag="U4A13_001891"
     CDS             complement(14421..15560)
                     /locus_tag="U4A13_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN1780172.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4333 a         3425 c         2841 g         5272 t
ORIGIN      
        1 tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat catctgaccc tcggtattag
       61 ctcaaaaaac ggcaaaatat gaggaactat tttccatttg tttctctaat actcaggaca
      121 aaacgctgat ttcacaacct ccgatatacg gtgttccaga agtagcttct tcgaaaaata
      181 agctgagatt tcacaaaaat ttgagaagca attttcgtaa attccttctt atgtcttgaa
      241 gctgaccact tctgtcataa cctcttttta ctgttcttaa acacggataa acatccgcat
      301 ctcattttat gagtgcttag atactttgat acgattgatt gctcggtgca acgcaacagt
      361 tgcccgtttc aattcgttcg tatcttgttt tgcttttgct tcttcgattt ggcgttctgc
      421 gcgttgtttt gcacgttccg cacgagatac atcgatatca cgttcacgtt ccgcactatc
      481 agcaacgatt gaaacaacat tatcacgaac ttccatgatt ccgccattaa cagcgatcca
      541 atcgacgtgg gtatctgaat ctgttcgttt tacacgaact tcatcaatcg tcaatggaac
      601 gatgattggt gcatgtttag gcaaaatccc aagctcacca gctgtcgtac gtgcaacgac
      661 gatagctgca tggtgatcat agaccaaacc ggcaggagtt accacattta ctgttaaata
      721 ttgatccatg gggcacccct ttctagtagc ctagtgtttt cgctttttct acgacgtctt
      781 cgattcggcc aacacttcgg aaagcttctt caggaagatc atcgtatttg ccttccaaga
      841 tttcgcggaa tcctttcact gtttcttcta cagggacata tgaacctgga agaccagtaa
      901 attgttcagc cacgttaaag ttttgtgaca agaagaattg gactctgcgg gcacgagaaa
      961 ctaacacttt ttcttgatcg gataattcat ccatacctaa gatagcaatg atatcttgta
     1021 attcacgata tcgttgcaat acgtgttgga cttctgttgc tactttataa tgttcttctc
     1081 ccacgatttc aggtgctaaa gcacttgaag aagaagccaa tggatctacc gctggataaa
     1141 tcccttgttc tgttaattta cgttccaagt tggttgtcgc atccaaatgg gcgaatgctg
     1201 ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata gattgcttgg atcgatgtga
     1261 tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg acccatttct gttgccaatg
     1321 ttggttgata accaaccgca gaaggcatcc gtcctaacaa ggctgatact tcagatccag
     1381 cttgagtgaa acggaaaatg ttgtcgataa acaacagtac atcttgacct tctacatcac
     1441 ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg catacgtgca cctggtggtt
     1501 cgttcatttg tccgaacacc atggctgttt tttcgataac tcctgaatct ttcatttcat
     1561 aatacaagtc attaccttca cgagtacgtt cacctacacc ggtaaataca gaaataccac
     1621 catgttcttg ggcgatatta tggatcagtt cttggatcag tacggtttta ccaacaccgg
     1681 caccaccgaa tagtccgact ttcccacctt ttaaatatgg ggcaagcaaa tcgataactt
     1741 tgatccctgt ttccaaaatt tctgtacttg tacttaattc gtcaaaggct ggtgcttttt
     1801 tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt ttctaaatcg atcgtatctc
     1861 ctagtacgtt gaatacgcga cctaatgttt ctttacctac aggaacagag attgctttgc
     1921 ctgtatcaat gacttccatt cctctttgta aaccatctgt tgattccatc gcaatggtcc
     1981 ggatcactcc gtcccctaat tctaacgctg cttcaagaac aacttttgat ttattttcgt
     2041 catttttata aacaactaat gcgttgttga tatctggtaa ggattggtct aaagaaaatt
     2101 ccacgtcgac aacgggaccg attacttgaa caatcttgcc tgaactcatc ttttttcctc
     2161 caatctcaat actattctag tgccgaagct ccggcaacaa tttcggtaat ttcttgggta
     2221 atagcccctt gtcttgcacg gttgtaagaa accgtcagct catcaatgat attagcagca
     2281 ttatcagttg ctgttttcat tgctgtcata ccagcagcat gttctgctgt tttggcgtca
     2341 acgatcgcac cgtagatcaa actttcagca tactgaggca ataactgaga caagatttct
     2401 tcttttgaag gttcaaaaat atattcttgt tcgaaagtag ttgcttcttc tggatctaag
     2461 tctgagattg gcagcatttt ttctacacga aactgactag ttagcgaatt gatatgatgg
     2521 ttataacata cgtataattc atcgaatact tcgttttggt acatagttgt tgccatattt
     2581 acgattttgc ggacttcatc aaagcttggc tgatcagata gattgcgcag ctcatatgct
     2641 agattgatac cgcgtgcttt aaagaagtct gcaccagttc cgccaattgc gatcatcacg
     2701 taatcatcgg gtgattggtg gtcttcttct aaaatagaca tcgtttgttt caatatcgag
     2761 ctgttatatc caccgactaa accgccatct gcagtgatga cgatataacc cgttttctta
     2821 acaggacggc tgagcaacat gctgttgtaa ttgatatccc ctctaggatt ttctgaggca
     2881 atatcattta actgagtagc agtcaaatgc gtaacgattt ctcttacttt tgttgcataa
     2941 acctgaaact tctgtgaaga agcttcagat tttgtcaact tagaagcaga caccatctgc
     3001 attgcccgcg tgatttggct cgtttttttc gttgaagcga tacgtgtctt gatttcattt
     3061 aatgaagcac ccattcatct cacctcacta tgcattttga atggatttca atttatcttc
     3121 tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct ttatattcct gaatcgcact
     3181 gttcaacgct tcttcttctg gaagatcttt tgttgtacgg attgtttcaa agatatctga
     3241 atgattggta tccagatatt cgaataattc atgttcgaag tctaaaatac tgtctacagg
     3301 gatactatct aagaatccat gtgtcaaagc atacaaaatc actacttgtt tttcaacagc
     3361 tagcggtgca tgcaattttt gtttcaagat ttctactgta cggcgtccac gatttagttt
     3421 tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta aatgcttcta attcacggta
     3481 acttgctaag tctagacgta aagtcccagc aacttttttc attgctttga tttgcgcaga
     3541 accccctaca cgagatacag atagtccggc atcaaccgct ggacgagtac ctgcgtagaa
     3601 caagtcgctt tctaagaaga tttgtccatc agtgatcgaa ataacgtttg ttgggatata
     3661 agcagagata tctccagctt gtgtttcaac aaatggtaag gcagtcattg aaccgccacc
     3721 taaatcatca cttaattttg ctgcacgttc caataagcgt gagtgtaagt agaaaacatc
     3781 ccctggataa gcttcacgac ctggtggacg gcgaagcagc aaggaaagtt cacgataagc
     3841 aacagcttgt tttgaaagat catcaaaaat gatcaagaca tgtttcccgt tgtacatgaa
     3901 ctcttcaccc attgcagtac cagcatatgg tgcgatataa agcaacggcg ctggttgaga
     3961 cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca tattttttca atgtttcaac
     4021 ttgtgtacga actgtagaat ctttttgtcc gattgctaca tagatacaga tcatatcttg
     4081 acctttttgg ttgatgatcg tatcgatcgc aatggaagtt ttccctgttt tacggtcacc
     4141 gataactaat tcacgttggc cgcgtccgat tggtacaaga gcgtcgattg cttttagacc
     4201 tgtttgcatt ggttcgttaa cagatttacg ttgcataacg cctggtgcca ttgcttctac
     4261 tggacgagct ttgtcagtaa cgatttcacc tagtccgtca attggctgac ctagcggatt
     4321 aactacccgt ccaatcaagg cctctccaac tggaacttcc atgatctttc ctgttcgttt
     4381 tactttatct ccttcacgaa tggactcgaa atcgccaagg ataatgatac ctacatcatt
     4441 tgattctaag ttttgcgcca ttccgtatga gccatttgaa aattcaagca actcaccact
     4501 catcgcgttt tctaaaccat gtgcacgagc gatcccgtca cctacataag ttacggtacc
     4561 gatttcttcg actgaaagca cgttttgata attttcaatt tgttctttaa tcaaggcact
     4621 aatttcttct gctttgatgg ccattcgatt cacctacctc tttgtactat ctatttaatt
     4681 gattacgcat attttctaat tgcgtgcgaa tgcttccgtc gattactcga tggttagctt
     4741 caacaatcgc gccaccaata atagaagaat caacgatttg ttttaattcg actgattgat
     4801 agttcattgt ttttgcaaca ttcttttcta attgtattcg ttgttcgtct gaaagtggaa
     4861 ctgctgttgt gacacttccc aacagcaaac cttgatgttc atcatatcga cgttcatatt
     4921 cttcgatcat gaacaaaaga tcatccattc ggttatacgc ataaacaact tctaaaaagt
     4981 ttttaacgat tccatcatag ccactaacaa gcttgtccat gatttctcgt ttttcatgag
     5041 gttctagacg aacatcactt aacatattac ctaatccagg tacttgttca tagatttgac
     5101 gtaagcttac gagatcttga taaatttctt ctgctttatt tgaatcgatc gccagttcaa
     5161 ataaagcttt cccgtaacgt cttcctactg tatatttatc tagtttcatt tgaagaacct
     5221 agaccttcga tatattgatt gattaatgat tcgtgcattt ctggagataa ttctttgttc
     5281 aagatttttt ccgcgatttg aagagatagt tctgcaacat cgtcttttac agagttcagc
     5341 gctgaatcac gttctacagt gatatcagct tgggctttac tttttagacg agctgcttct
     5401 tcttgagcat ccttcaaaat attttggcgg cttaattctc cactttcttt cgcatttttg
     5461 atgatatcag ctgcatcaga acgagaggct aacaattgtt gttcgcgttc ttgttccatt
     5521 ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat cattggcgat cttgtcttca
     5581 cgttttttca aaatatcgct gattggtccc caagcaaaat gcttcaagag agccagtagt
     5641 atcaaaaatg agccgctgac aacgatgaca tttcctaaca tcgcattgcc aacttctgca
     5701 attgccaatt gattcagcat aggcgagcct tcctttcttt gaagtacttc tagttttcat
     5761 gtaaaaaagc agtaacaaac caaattgttt ctgccttaat actgattcaa ttaaacacca
     5821 aaaactagaa tcaaggcgac aactacccct aagataggaa cagcttcgac tagagctacc
     5881 ccgataaaca ttgttgtacg gaattgacca gacatttcag gttggcgagc cattgattcg
     5941 attgttttag agataacttg accgttacca taaccggctc cgattgctgc tcccatgatt
     6001 gcgattgctg ctgcgatata attcataatt gttttcctcc taaattagat aaatagtagt
     6061 ttgtttttaa aacgaatatt attcttccac ttcaatctta tggctcatat aaaccataga
     6121 taaagtaacg aagacaaatg cttggatgct tccgataaac aaggagaatg cgatccagac
     6181 catctctaat ggaattgcta acggcaatga gaaccagcct aagcttatta ccatttttgc
     6241 aatcaaggtt agcaaaactt cccccgcata aatattccca taaagacgta aagcaagtgt
     6301 taataagttt gtaaattctt caatcagttt catcggaaac ataaaactat atggtttcaa
     6361 gaacgaattt acgaaatatc ctttgaagcc taactttttc accccaaata agtgagtaag
     6421 taagatcatt ataaatgcca aagttaaagt aacaaacggg tctgctgtcg ggcttttcca
     6481 aagtgttgtt tcgttaggta aaacgatttt tgttactaaa ccgatgttgt ttgcaacaaa
     6541 aacaaataaa aacaatgtaa atgccaacaa atggaaattg ctcaattctt ttctaggcag
     6601 attatctgtt acgattcctc gactaaaatc aatgacccat tctatcgcat tttgtttgcc
     6661 tgttggtttc aacttaagat ttcttgtaaa aaagtaagct aagaagaaaa caatcaaaca
     6721 ggtcagtaac accattatac aaactgtacc atcaaaccaa attggtccaa tgtggaacgt
     6781 tagcgagcgt tcatccaatc catttcacct cttttccccg aaattcacat aaaatcaatt
     6841 catgtgcgta tctggttttt tccaatcggt ttctaaccac acggtatcat accaccatga
     6901 aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt ttttatttca gtcaaaaacg
     6961 aagcgaattt ttctttatac acggtgctcc tgatctaaga aaaaacctat gataatcatg
     7021 ttttatcaga ttaaatgcta acaaatttta tcattaagta aaagcaaaag caccgaatat
     7081 gcgaaaaagg ataatgaaat tatctagaga aattttttaa tgttcgatca atttgtcgat
     7141 cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga ttttttccct ttcgccagac
     7201 caatcaatac cttggcatag ccgttacgaa tatagacttt taaaggaaca acagtgattc
     7261 cagtattttt caattcattt tcaagccgga tgatttgttt tttgtgtaat aataactttc
     7321 tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc ataaggactg atatgaacat
     7381 tatgcaagaa agcttctccg ttgcggacgc gaataaatcc atcttttaga ttgatccggc
     7441 tgtttcgtat cgacttgatc tcggttcctt gcaagaccat ccctgcttcc atcgtgtcga
     7501 tgatcgaata atcatggcga gctttcttgt tttgtgcaat taattttccc tcgccttttg
     7561 gcatagctgt ttcctccaac tcattttatt ttttcttttt cttcttttta acacctttgt
     7621 aaaatggctt cttccctttt ttcttaccga acgtttgctt actatcgcta tatcgtttgt
     7681 tcttcttgcc atttctttct gggcgttttc ctgcttgttt tccttcgcgt tttcctgatt
     7741 ttccatttcg tgtttttggc cgttccaacg ctgtaacctc ttctgcggaa acaagttcaa
     7801 aatctactgc tcgttcatct ggatcagctt tgactacttt tactcgcact ttctgtccga
     7861 ttttaaaagt cagaccagtt ctttcaccta caagagctaa atgattttca acaaaatgga
     7921 agtagtcctg tttcaattca ttgatatgga tcaatccctc tactgtattc ggcagttcga
     7981 taaacaggcc gaatttcaca acagagctga tgatcccatc gaattcttcc cccactttgt
     8041 ccatcatgaa ttctgctttc ttcatggaat ctacttcacg ttctgtttct actgcacgac
     8101 gttccatctt agaactatga tctgcaattt caggaagttc cgtagcccac gaactttgat
     8161 tgctttcact tttatcttca ctgtaagaac ggatcagacg atggacgatt agatcgggat
     8221 agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc cgctaagcca taatgcccaa
     8281 aattgtcttc tgaatagcgt gcttgttgca tgcttcttag caacatagta ttgataactg
     8341 cagcttctgg tttgtcttct actccttcga tcacatgctg taaatctttt ggtgtgatcg
     8401 ttcctttcgt tcctcggacc agaatgccca aagctgaagc aaaatcaaag aagcgctgca
     8461 ttttttcttc ttttggctgt tcatggattc ggtaaataaa cggcagattt aatttattat
     8521 aatgttcagc taccgtttca ttggccgcaa gcatgaaaga ttcgatcaag cgttcaccaa
     8581 ttcctctgct tcttagctgg atatcatttg ggtgtccttc agcatcaaca aggatttttg
     8641 cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg catcgtttca aggattcgat
     8701 gcaattcacc catttcttgg aacataggta cgagctgagc atagcgttgc atcgtttctt
     8761 cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt catccgttct gttgtttgga
     8821 tcacacttgg gaaaatatca tgagagacta cttgtccatt tggatcaatc tccatttcgc
     8881 aactcatggt cagccttgga acatggggat tcagtgaaca gataccattc gataaacgtt
     8941 gcgggatcat cggaatcact cgatcagtca aatatacact tgtccctctt tcatatgctt
     9001 ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac atctgcaatg tgtactccaa
     9061 gaaaataatt accgttatct aattttttaa ccgtaactgc atcatctaga tcttttgcat
     9121 cttctccatc aatcgtaaca atgagctgat ttcttaaatc tctacgtcca gttatatcct
     9181 tttcgctgat ttgatctgga acttcatctg cttcagacaa tacatcatca ggaaatttcg
     9241 tagggatacc attagccacg acaattgaga ggatatccat gccagggtca ttcttatgac
     9301 caatcacttg cttgattatc ccttctaaac tagtcgcata ccctttttca gggtaatggg
     9361 tcagttcgat cagcacaata cttccgtcaa ctggacgaac tccctctgct gctgcatata
     9421 ctttaaattg gttgagcttt ctatctttcg ggataacaac gccgtaaaga tccgtttctt
     9481 gtatttcttc ttctgaataa gcaacgaatt cccctgctaa ctgagtagtc gcccggtctc
     9541 gaatctcgac tactttcccc tctgctccgc gatccatatc tgtatttgct cgcttcatga
     9601 tatcgattgc tacggtgtct ccatccattg caaaaccagt cgcttctttt gggatataga
     9661 tgtcatcttc ttcgggatca atcgttacaa atccaaatcc tcgttcattt gctcgaaatg
     9721 ttccttcgat caagattggt tttaaaggaa gtttcacttt tcctttttta ttgaaaacta
     9781 tactctgttc tctttccatt tgagcaatcg tttgtactag taatttaaaa tcatcgcttt
     9841 tttggaaccc taaaccttca gcgatttctt ccatagaaaa actgcgtttg cgactgcttt
     9901 ccatgaaaaa gagaattttc tcttttatcg tttcttttgt catatttatt cctcattcca
     9961 aggtaatttt tctaaaaatt ccaagacgtc tttttctaat tcgcgtcttg ctgtaccaac
    10021 tgtaatcaca tgaccacttt caggatacca tcgaagtgtc actcgctgac ggctcaactt
    10081 gcgtgcagtt tcaaatacgc cattagggtc gatcatttca tctttcccag cttgagctaa
    10141 aaatatcggc tgttggatat ctgacaacct actttctacg attgctgcct gatcttgtat
    10201 atccattaat tgttgttcag ctaacgagcg gtaagcaact gctttttcat tgatttcttt
    10261 ctctgattta cctgctgtct tcagcacttg acgtacgtat ttttcaaagt tttcaggcac
    10321 gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg ccgccaatca ctgattcttc
    10381 ttctagtgct cgtacagcaa agatgccgcc cattgataat cctaatacgg caatatcaga
    10441 aaactgctca gactgtaaaa aatggatggc ttttttagtg tctgcccacc agctttctgc
    10501 tttttcttgc aaaatatcat atggctccat tgttccgtgt cctttaaaaa gcggcgcata
    10561 aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt cgaacatcgt ttggacttcc
    10621 tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt tttgcataaa ggggttttgg
    10681 taattgtctc actcgtctca cctctttcgc ctcattttac catagtttct ttcataactt
    10741 cttcttaagc gttctctttg caataaaaaa agccgcccgc cgttttgcga gaggcttatt
    10801 caactacttc gaagataaat atgctaggac aaataataag atcatccaga tagctcccat
    10861 aactgcagtt gatcgctgca tgacagcttc aaagccgcga gctttttgtt tgccgaatag
    10921 ttgatccgca ccgccggtaa atgcactcgc tgcactattt tgcttgcttg gctgcatcat
    10981 aatcgcaatg accataacaa tcgataaaac gatcacgatt cccaaaatta aattatacat
    11041 atcgcagcct ccttcccaaa agatatcgtc tctttaactt tatcatagat aaaagtccgt
    11101 gacaactcta tataaatgga ataacagctg aaagttcaaa actttcagct gttattctta
    11161 tgactgacgt aagacgtctt gatattctgg tttcttttca aactcttttt ttgcataagg
    11221 gcataaagga atgatctttt tcccgtctcg tcttgctaat tctactccag tatacactag
    11281 cttttcagct aacttttgac cacgatatgc tggatctaca aatgtatggt caatgatcat
    11341 gatgtctggg cccgcatctg accatgtcat ttctccgatc tcttgttgtt catcattgaa
    11401 taaagcaaga cgattttttt cttctttgat ttccatatgc cgcactcctc aaataactga
    11461 tattttttta ttcctacctt attttagcag tctcctgatc tataaacaat tatttatcct
    11521 cgaattactt acaaaaaaga agagaaaatt aattcatcgt tattctttgt tcaataaatg
    11581 aaaaaatcac tgtccaatag gttttcggat ctttcatcga tgcttccaag tgttctgctt
    11641 ctggaacaag cagcagtgct ttatcccctg ccgtagcatt ttgaatcgtg tacgctgctt
    11701 ctgtagggac aaaatgatct ctttcaccat gaatgaccaa taaaggcaga tggttgctgc
    11761 caagctgtct cgtagctgat gcttgcttta aggagtaacc ggcgtatttc tgggcataat
    11821 gattcgcata tctcatgacc atttttttag gaaagacagt gattttactt aacatatatc
    11881 ggaattctgc ataaacagaa acatatccgc tgtctaaaat aaatcctttg accgctgatg
    11941 gaagtttttc accacttgcc atcatgatcg tagaagctcc cattgatccg ccgtgtaaaa
    12001 taatggacgc attcggctgt tcatctaaga tgagttggat ccaggcaatt aaatctagcc
    12061 gatctagcca tcccattccg atgatttctc cttcactttc tccgtgcgca cgtaaatcag
    12121 gtacaagaac gttgaatcct ttctctgcat atcttttacc tatatgagac atgtctcttt
    12181 tccctgtgct tcggtaatca tgtacgcaaa tgacccattt attctgctta tttggatgga
    12241 tgaacatctg accagctagt ttcaacccgt cataactttt gattgttttg ggtgttccct
    12301 gctcccaaaa gctttcacac tcttcttcta atttttgtcg ttttttttct actgacgtga
    12361 ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata ccagtcatat cctctctgta
    12421 ctcccgctct tactaatttc tttgcataaa aataagaaag aaaatggtaa ctgatcccta
    12481 taattatcag aataagaatg aaataaaaaa taaccattgt acacccgccg ctttctgcca
    12541 aactataatc taactattat ccaatgtacc gatccatagg attctttatt ttcggcacac
    12601 gtaaattcca tttctttttc attgtatcat ttacttaaag ttctcctcaa tagatttact
    12661 tgtaagagag catttacatt cgcaataaaa gccagtatac taatttcgtg cattataatc
    12721 aagacgctcg tcttggactg gttcggaaac ttcccagaat aaaaaactaa gtatctctaa
    12781 agaaaggaga ttggaaataa tgcgaaaagt aattattgat tgcgatccag ggatcgacga
    12841 cactttagct ttatcattag ctgtcaaatc ccctgatatc gaagtagttg ctattaccgt
    12901 agtttgtggg aatgtccctg cagatatagg tacgcagaat gtgctgaaat gcctggaacg
    12961 ctgtgatcgt ttagacattc ctgtttatca aggaatggaa aaacctttga agcaaccttt
    13021 catcagtgca caagacactc atggtatgga tggtttaggg gaaacaaatt tcccgatgat
    13081 cttgagaaaa caggcagaac ctctccatgc cgttgatttt ttggctgatt atttcaaaga
    13141 aaagacaaat acatcagtca ttgctttggg tcctttgacc aatatcgctt ttgctttgaa
    13201 agtcaaccca aatattggga aacatatgga tcgttttgtc tcgatgggtg gaacttacaa
    13261 aagccatggt aactgttcgc cagttgctga atacaattac tggtgtgatc ctgaagcagc
    13321 ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg gttgggttag atgtgacaag
    13381 agaaatcgtc ttgacaccga ctatcctcga atactgctgt caaatgaatc ctgaagaggg
    13441 agagtatcta aaagcgatca ctcgttttta ttttgacttc cactggaaac aagaaagaat
    13501 tttaggatgt gtcatcaatg atccgttagc tgttgcttat tttatcgagg gggccatctg
    13561 tgaaggtttc aaaagcttca ctgctgtgga aactcaagga ataagtcgtg gacaaacatt
    13621 ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt aaaattatga caaaagtaga
    13681 tactcgtctt tttttccgaa agtttttaac cgttctttta aatgcacaag aagaaacgat
    13741 tatgaaagat ttggaaaggt taaagatggg ctaacaaaat gaataaaaac aaaatggcaa
    13801 ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa ttatattgga ggaacgattg
    13861 ctttatggct tcgtttgcca atctatctcg attctatcgg aacgattttt gctggtgcat
    13921 tgcttgggcc tgtacctgga atgctgacag gattgtccag cggtatttta agtggagtga
    13981 ctacagatat tttttctctg tattattcgc caatccaaat tctcacaggc ttgcttgctg
    14041 gattgatttt gccaagatat ctcggcaaag ataaaaacag cttgaaactg ttattactcg
    14101 ctttgatttt gtcggtacct ggaacgatcg tttcttcgat cattaccgtt cagttatttg
    14161 gcgggatcac ctcctcagga tccagtatga tcgttcagct attacatggg ttaggtttaa
    14221 gtcagacaac aagtgtgatc ttggtacagg caggtacaga ctaccttgat cgtttgttgt
    14281 ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca acgcttgata tattcaaaaa
    14341 gaaaaatagg ctgaataaac agagctggat tagcaaaata atgctaatct ggctctgttt
    14401 tttcatttat taaaaaaatc ttacgctaag taggataaaa taattccttg atatttttca
    14461 atcatatcca aataattatc gatttcaaca tactcatcta cttgatgagg caaagtaacg
    14521 acaccaggcc caaagacgac aaagtcgaag gaatggttag ctttcgtaaa ttcggctgca
    14581 tccgttgttg ctgccgctcc aactaaaggt aatggctggc tgaattgttg ctgaatgcaa
    14641 tgaatcaaag gagaatctgg atcagctttg acaggtattt tgttgtaatc gatcatcaat
    14701 tccaaatgat agtctgtctc ttgattcaat tcattgacga ttgattgcag caatgcaatg
    14761 attttatcat ttgggtattc tggaatcgat cgaatgttcc cttggagctg cgcatggctt
    14821 gggatgctgt tgacttgatt cccgccactg atcaatgtaa cattatggat tgtttttcct
    14881 aggacaggat tctcgattgt ttcagcgagg tgattcattt cagcatttgc tttcgtaatg
    14941 aattcattta ggtgattgat tgcattataa ccttggtctg gcatagagct atgcgcttct
    15001 tttccgtgcg aggtgactgt atagttgatt gatcccatgt gcgtatacat caaactgtaa
    15061 ttagttggct ctccgatgat caaggcatct aagtcatcta catagcctgc tttcgtgagc
    15121 tgttccccac ctagttcacc cacttcttct ccgacagtgg ctaataattt gactgttccg
    15181 ttaaatggtt ttcctgactc tttcagttcg atcatggcaa tcaccattgc tgccaagcca
    15241 ctcttcatat ctgtagcacc acgtccgtac aaacggttcc catggatttc tgctgcaaag
    15301 ggtgcatagg tccaagacga ttcatcacca gccgccacga catccatatg cccagatagg
    15361 cctaacactt ttcctgattg gcccttttgg taacgagcaa tcaaattatc tctaccatct
    15421 ctgtaagaaa caatctctcc tgttatgtca tgctttgcca aaagcttatt caaataggct
    15481 gctacttcac cttcgtttcc attcactgat ttaattcgga tgatctcttg taaaatcgcg
    15541 attttttctt cttttttcat ctaatcctct cccttgccat ttctttttat tgtatttgag
    15601 tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat gaagaaagag agccacttta
    15661 tgaaaaatat gctgctactt ccctcctatt ttatttatac gagaggactt gctcatagat
    15721 tcctattcta ctgttctatc cttaaaagct cttatatgat gctagttatt gttagctttc
    15781 ctttttcaag gatgatcttt tcttgtacca ttctttgtaa acatcatata cagaagctag
    15841 ttgcaaaaaa aactctctac ctgctgatat t
//
LOCUS       DASJPC010000060        15687 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJPC010000060 DASJPC010000000
VERSION     DASJPC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.060"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..751
                     /locus_tag="U4A13_001892"
     CDS             68..751
                     /locus_tag="U4A13_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN1780173.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="U4A13_001893"
     CDS             753..1589
                     /locus_tag="U4A13_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN1780174.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="U4A13_001894"
     CDS             1600..3354
                     /locus_tag="U4A13_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1780175.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="U4A13_001895"
     CDS             complement(3630..3971)
                     /locus_tag="U4A13_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN1780176.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="U4A13_001896"
     CDS             complement(4039..6210)
                     /locus_tag="U4A13_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN1780177.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="U4A13_001897"
     CDS             6379..7278
                     /locus_tag="U4A13_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN1780178.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="U4A13_001898"
     CDS             complement(7349..8206)
                     /locus_tag="U4A13_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1780179.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="U4A13_001899"
     CDS             complement(8191..10893)
                     /locus_tag="U4A13_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN1780180.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="U4A13_001900"
     CDS             complement(10906..12195)
                     /locus_tag="U4A13_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN1780181.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="U4A13_001901"
     CDS             12345..13391
                     /locus_tag="U4A13_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1780182.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="U4A13_001902"
     CDS             13393..15546
                     /locus_tag="U4A13_001902"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN1780183.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DASJPC010000061        14738 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJPC010000061 DASJPC010000000
VERSION     DASJPC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.061"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            74..970
                     /locus_tag="U4A13_001903"
     CDS             74..970
                     /locus_tag="U4A13_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN1780184.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1120..2061)
                     /locus_tag="U4A13_001904"
     CDS             complement(1120..2061)
                     /locus_tag="U4A13_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN1780185.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2187..3308
                     /locus_tag="U4A13_001905"
     CDS             2187..3308
                     /locus_tag="U4A13_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1780186.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3566..5068
                     /gene="eat(A)"
                     /locus_tag="U4A13_001906"
     CDS             3566..5068
                     /gene="eat(A)"
                     /locus_tag="U4A13_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1780187.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5096..5662
                     /locus_tag="U4A13_001907"
     CDS             5096..5662
                     /locus_tag="U4A13_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1780188.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5873..6934
                     /locus_tag="U4A13_001908"
     CDS             5873..6934
                     /locus_tag="U4A13_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1780189.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6937..7833
                     /gene="murQ"
                     /locus_tag="U4A13_001909"
     CDS             6937..7833
                     /gene="murQ"
                     /locus_tag="U4A13_001909"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1780190.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7847..9829
                     /locus_tag="U4A13_001910"
     CDS             7847..9829
                     /locus_tag="U4A13_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1780191.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9894..10340)
                     /gene="nrdI"
                     /locus_tag="U4A13_001911"
     CDS             complement(9894..10340)
                     /gene="nrdI"
                     /locus_tag="U4A13_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN1780192.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            10551..11975
                     /locus_tag="U4A13_001912"
     CDS             10551..11975
                     /locus_tag="U4A13_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1780193.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(12156..12929)
                     /locus_tag="U4A13_001913"
     CDS             complement(12156..12929)
                     /locus_tag="U4A13_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN1780194.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(12926..13831)
                     /locus_tag="U4A13_001914"
     CDS             complement(12926..13831)
                     /locus_tag="U4A13_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1780195.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(13985..14305)
                     /locus_tag="U4A13_001915"
     CDS             complement(13985..14305)
                     /locus_tag="U4A13_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN1780196.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         4718 a         2618 c         3111 g         4291 t
ORIGIN      
        1 acgttttact ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag
       61 tgaggcggaa aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta
      121 ttttttgcct tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga
      181 attcattaaa tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta
      241 tggatttgat atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt
      301 atttttttgg aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga
      361 accggcaatc gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa
      421 acataatagt tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga
      481 tctatttttt ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga
      541 atttataaaa agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca
      601 acctggatat cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct
      661 ggaactgttg cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt
      721 tcaaaagaaa ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga
      781 aaatattttt ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc
      841 atttaagcca atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg
      901 atttatcact aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga
      961 aaacaaataa aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc
     1021 ttatggcatc atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga
     1081 accagctgct gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc
     1141 ttcttttgca tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt
     1201 ttgtcctttt cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt
     1261 gatcaggtct tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga
     1321 agcaatatgt tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga
     1381 agcaaattca gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc
     1441 agcaatatta caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt
     1501 tcgccaaatg gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa
     1561 gacttctaca actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat
     1621 ttctccatca cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt
     1681 gatcccaaat aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa
     1741 tccatttagt aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa
     1801 catatcatct aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct
     1861 ttcgatgatt tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa
     1921 gtcagcaatc aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa
     1981 gaggacctca tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc
     2041 cccagctatt gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta
     2101 tatagaaaaa tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt
     2161 acttaagaag aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag
     2221 gaagtgcttc ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag
     2281 gaagaaaaat caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt
     2341 acgcgctcag gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa
     2401 aaatcagcta tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac
     2461 aagcattggg gttagatcta cttgataaac caacggatca aactatccga cttgcttcca
     2521 gttatgcctt aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg
     2581 tcactttagg aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg
     2641 cttctttaga agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg
     2701 atcgaagaaa cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc
     2761 catatacagg tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata
     2821 cgacgctagc tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct
     2881 gggaggtgga tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc
     2941 tttttttgtt gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat
     3001 tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc
     3061 aaacgatcca cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac
     3121 caataatcgc tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc
     3181 caattgtctt aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc
     3241 aaacattaga aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc
     3301 cgaagtagtt gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt
     3361 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
     3421 tgtggagaca aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg
     3481 ggtctttttg tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg
     3541 gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg
     3601 ctacgacagc caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg
     3661 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca
     3721 aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca
     3781 acagacaaaa gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga
     3841 gatatgggaa atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag
     3901 agaattcagc acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt
     3961 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggtag
     4021 aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga
     4081 ccggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga
     4141 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     4201 aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga
     4261 aaaagccgaa tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat
     4321 cgatactgaa tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat
     4381 gaaacgatcc aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact
     4441 attaaaagat atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa
     4501 gcgactttta agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat
     4561 tcattttaca atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa
     4621 gtcatccatt atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata
     4681 ccagccaaaa catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac
     4741 gttggcggaa tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg
     4801 aaagcttggg atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca
     4861 acggaaaaaa gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga
     4921 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa
     4981 cgtgaaacct gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac
     5041 tgagattatt tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt
     5101 taaggggaac tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     5161 aacaacttgg aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa
     5221 aggacagatg caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac
     5281 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg
     5341 tctcagcttg ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga
     5401 tggactgcca agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt
     5461 cccaaatggg gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg
     5521 catcgaagac gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat
     5581 caatgcttat tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac
     5641 agaattggtt tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc
     5701 actgcttttg cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact
     5761 caacctttag aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag
     5821 atatatactc tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg
     5881 cttttcaata tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat
     5941 ggcatccaat ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc
     6001 ggcatatcaa aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt
     6061 gatggtcgat atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac
     6121 gcctttgaaa gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca
     6181 gattgccgac tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa
     6241 agatatcgat gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa
     6301 ttattatcca cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg
     6361 gctcaaaatt caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg
     6421 cccactatat caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg
     6481 cgctctagat ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc
     6541 agaaaacgtt caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac
     6601 ggaacctgta gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga
     6661 cgatgcaaga gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc
     6721 agcaagaaat acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt
     6781 gcgctatatg ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa
     6841 tgtagtagct aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat
     6901 gaactatcaa tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac
     6961 ctaacaacgg aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa
     7021 gccgttcaac taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg
     7081 acaagtatcg aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg
     7141 atttatatgg gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta
     7201 ccgacattcg gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca
     7261 atgactgtag ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc
     7321 aagctgaatt tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca
     7381 tatgtgatcg gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct
     7441 tgcaataaac aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt
     7501 ccagaatttt tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg
     7561 aatatgattt ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc
     7621 gatgtcaaac caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg
     7681 acagaagctg attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag
     7741 ctggctatcg tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct
     7801 gaagcaaacg gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga
     7861 actgactttg gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt
     7921 agaaagcatt gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt
     7981 aaatgtctct gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct
     8041 tcaagtcatc atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc
     8101 aggcgtgaaa ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc
     8161 atctggaaaa gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa
     8221 acaaaaacca tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat
     8281 gattcctgca tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt
     8341 agtcacagcc ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat
     8401 cttgaaaaac gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt
     8461 ttttggtgca acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat
     8521 gaatccggaa gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg
     8581 tgggatccta ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca
     8641 taaagtagta cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat
     8701 cggtctgatt gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt
     8761 cggtgggatc aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac
     8821 attattcttg ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca
     8881 aatgattgat gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg
     8941 acaagtgggt gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga
     9001 aatgatcaaa ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg
     9061 agtaacatta cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg
     9121 ggcagtgatc ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc
     9181 tttgatccca ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc
     9241 ttatgctggt ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa
     9301 agaagaatta gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca
     9361 aacttcagct gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt
     9421 agataaagca agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga
     9481 accagaaaac ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac
     9541 gaaacacgct attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat
     9601 caacacagtt gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt
     9661 gacacctgat acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga
     9721 aacatccatg atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa
     9781 aacaggtaaa gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa
     9841 caatagaaac gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta
     9901 ttgttttctg ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca
     9961 taatctgcca agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac
    10021 tgattattga agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct
    10081 tcatgttcca gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa
    10141 ccatttccgc cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta
    10201 aaatcactat tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca
    10261 tgcatttttt cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc
    10321 gatatatata aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt
    10381 tttaagtttt ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga
    10441 aaaaagccgg tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa
    10501 ggtttgttat actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag
    10561 agcaacagaa aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga
    10621 ttggaggtgg cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta
    10681 ttttcagttg ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata
    10741 tcaataaaaa acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg
    10801 gccgtttcgc tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg
    10861 tagcttttgc aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg
    10921 ggcaaaatct ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag
    10981 tgaatcgggg agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag
    11041 tgccattatt tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg
    11101 ataacttttc tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc
    11161 aagtattgag ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg
    11221 tcgtaggttc acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat
    11281 tcggcttgat tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga
    11341 atcagctgca aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg
    11401 gtaaatgggg agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc
    11461 tctcatggac gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc
    11521 ctaaagcatg gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga
    11581 cgttatgtac gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg
    11641 cttacacact tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa
    11701 tgaaactttc ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg
    11761 cctttcaagt atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc
    11821 ttttcttgcc aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac
    11881 aacatatcca cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa
    11941 tggttttatt tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag
    12001 gttgtgagcc tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg
    12061 tcacagctcc tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg
    12121 aacgtctctt tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg
    12181 agatatccta atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta
    12241 gtagaaagat gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc
    12301 aacatactga tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata
    12361 ccagatatga ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa
    12421 ctattgataa agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa
    12481 ctacccaaag tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc
    12541 gtgaaggaaa tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa
    12601 aggtataaat aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct
    12661 cgagaatact ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg
    12721 atcgtgcttg cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga
    12781 gcagtcattt caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct
    12841 atgagcaaaa caactaatag aacagataaa acagcagttg actttttctt gatcaattta
    12901 aataattctt gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa
    12961 tttccaatag agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat
    13021 tttgtgcaga caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct
    13081 tgattttatc aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta
    13141 gatcatttgt tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg
    13201 tacattgttt gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa
    13261 gctcgctgag taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa
    13321 tgatttttcg cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa
    13381 taacgataga cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca
    13441 acgagtagga tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag
    13501 tgtcgatagc agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta
    13561 agaatggata gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac
    13621 tagagggaga aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc
    13681 ctaaaatagc tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga
    13741 ttttgccagg ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct
    13801 tggataattc ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta
    13861 caagcataca cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt
    13921 cataaaaaaa ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa
    13981 gtgtctagtg gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa
    14041 agaaccaagt ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac
    14101 caactgcgcc aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg
    14161 cctttgacaa aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga
    14221 ttttagaaaa accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg
    14281 ctatgattaa ttccatccat gccatactta cattcctcct taactataaa aaaatacaca
    14341 aaaagaggaa aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc
    14401 ggtggcagat tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct
    14461 catacttttt agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat
    14521 gaatgctttg caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt
    14581 aactagacct tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa
    14641 taagtaagat aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt
    14701 tcgtgagaaa acaactatat tttttatgtg ttttgctt
//
LOCUS       DASJPC010000062        14482 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJPC010000062 DASJPC010000000
VERSION     DASJPC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.062"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(287..649)
                     /locus_tag="U4A13_001916"
     CDS             complement(287..649)
                     /locus_tag="U4A13_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780197.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="U4A13_001917"
     CDS             complement(723..1103)
                     /locus_tag="U4A13_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN1780198.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1167..2360
                     /locus_tag="U4A13_001918"
     CDS             1167..2360
                     /locus_tag="U4A13_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1780199.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2437..3021)
                     /locus_tag="U4A13_001919"
     CDS             complement(2437..3021)
                     /locus_tag="U4A13_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1780200.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="U4A13_001920"
     CDS             3222..4220
                     /locus_tag="U4A13_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1780201.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="U4A13_001921"
     CDS             4332..4841
                     /locus_tag="U4A13_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN1780202.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="U4A13_001922"
     CDS             complement(4871..5530)
                     /locus_tag="U4A13_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN1780203.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="U4A13_001923"
     CDS             complement(5532..6296)
                     /locus_tag="U4A13_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN1780204.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="U4A13_001924"
     CDS             complement(6304..6981)
                     /locus_tag="U4A13_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN1780205.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="U4A13_001925"
     CDS             complement(7018..8403)
                     /locus_tag="U4A13_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1780206.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8372..8626)
                     /locus_tag="U4A13_001926"
     CDS             complement(8372..8626)
                     /locus_tag="U4A13_001926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780207.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="U4A13_001927"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4A13_001927"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="U4A13_001928"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="U4A13_001928"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN1780208.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="U4A13_001929"
     CDS             9342..10199
                     /locus_tag="U4A13_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780209.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="U4A13_001930"
     CDS             complement(10280..10615)
                     /locus_tag="U4A13_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780210.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="U4A13_001931"
     CDS             complement(10682..12052)
                     /locus_tag="U4A13_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN1780211.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="U4A13_001932"
     CDS             complement(12130..12666)
                     /locus_tag="U4A13_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1780212.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="U4A13_001933"
     CDS             12975..14456
                     /locus_tag="U4A13_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780213.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4461 a         2883 c         2353 g         4785 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctctta tt
//
LOCUS       DASJPC010000063        14342 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJPC010000063 DASJPC010000000
VERSION     DASJPC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.063"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..489
                     /locus_tag="U4A13_001934"
                     /pseudo
     CDS             108..489
                     /locus_tag="U4A13_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            620..1570
                     /locus_tag="U4A13_001935"
     CDS             620..1570
                     /locus_tag="U4A13_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1780214.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2088..2543
                     /locus_tag="U4A13_001936"
     CDS             2088..2543
                     /locus_tag="U4A13_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1780215.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2941..3486
                     /locus_tag="U4A13_001937"
     CDS             2941..3486
                     /locus_tag="U4A13_001937"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780216.1"
                     /translation="MELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVA
                     ELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTFKRLWINSLEKDVIRSGFQN
                     LQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLI
                     FQRQEAIENFKKEPFFEVEAK"
     gene            3580..3912
                     /locus_tag="U4A13_001938"
     CDS             3580..3912
                     /locus_tag="U4A13_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEN1780217.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4063..5042)
                     /locus_tag="U4A13_001939"
                     /pseudo
     CDS             complement(4063..5042)
                     /locus_tag="U4A13_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5047..5814)
                     /locus_tag="U4A13_001940"
     CDS             complement(5047..5814)
                     /locus_tag="U4A13_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1780218.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6304..6729
                     /gene="lacA"
                     /locus_tag="U4A13_001941"
     CDS             6304..6729
                     /gene="lacA"
                     /locus_tag="U4A13_001941"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEN1780219.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6746..7261
                     /gene="lacB"
                     /locus_tag="U4A13_001942"
     CDS             6746..7261
                     /gene="lacB"
                     /locus_tag="U4A13_001942"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEN1780220.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7272..8204
                     /gene="lacC"
                     /locus_tag="U4A13_001943"
     CDS             7272..8204
                     /gene="lacC"
                     /locus_tag="U4A13_001943"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEN1780221.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8207..9187
                     /gene="lacD"
                     /locus_tag="U4A13_001944"
     CDS             8207..9187
                     /gene="lacD"
                     /locus_tag="U4A13_001944"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1780222.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9215..9532
                     /locus_tag="U4A13_001945"
     CDS             9215..9532
                     /locus_tag="U4A13_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1780223.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9532..11238
                     /locus_tag="U4A13_001946"
     CDS             9532..11238
                     /locus_tag="U4A13_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1780224.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11380..12786
                     /gene="lacG"
                     /locus_tag="U4A13_001947"
     CDS             11380..12786
                     /gene="lacG"
                     /locus_tag="U4A13_001947"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1780225.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12809..13081
                     /locus_tag="U4A13_001948"
     CDS             12809..13081
                     /locus_tag="U4A13_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEN1780226.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13102..14001
                     /locus_tag="U4A13_001949"
     CDS             13102..14001
                     /locus_tag="U4A13_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN1780227.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4685 a         2363 c         2765 g         4529 t
ORIGIN      
        1 gacactatcg ttcttcatca acgtaagtta gatttgttga aagagcagaa aaaaggattt
       61 ctacaaaaaa tgtttgtttg atattttaaa tagaaaagag agataaagtg aagtttttaa
      121 attttttgaa ctataaatat ttaaactggg tattatatgg tattacatta ttttttatta
      181 tttattactt ttttaccgat gatgatattt ttttaatttt agcaatactt tttcttgttt
      241 catctaattt tagtgataac ttaattgaag ttcttacaga accagaaatt cggtctatga
      301 cggatgaaga acaacagatt attaaagcta tgatttcgga aaataagaaa gacgtagtag
      361 ttatcaaaaa gattagagag tatacaaatt taggtgtttt acaagccaaa aaagtatatg
      421 aaaaaaaaaa aaagggtcta taattacaaa ttagataata cattagaagc tggagacaag
      481 attgtctaga taatgtgtga gataaaattt ttggcaggtt atcatacata agacaaaatg
      541 tttttaaaac tgataacgta tttattccac aacaattacg catttatttc tgagaaaaat
      601 ataaagcagg aggattacaa tgctgaaaac ttattcctta acaaaagata cattattagt
      661 ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat gtttatggtg acgaacgaga
      721 atatatcgaa tcttctgaac ataaatatga ctttaaaata ggcaatatac taacgtttga
      781 tgatcgagtt gcatatgaca cgatgataga taagaacgat gaaaaaagtt tgctcgtaag
      841 ttttttgttt cccgaggtac atgaaggtgg gcatctagat accctgacga catctgttgt
      901 tttcttggta tttaaaaata agttgataat ttttacaaaa caaaaattaa aattcattcc
      961 cgaactctta tccaatatgg ttttacgtga tgtcaatcat ttcgtggagg aagtactttt
     1021 aaagatcatg caaaaagatt tctatatgat gcttaacgac cttcgtggag tcaaggaaaa
     1081 aattgatgat ttagactcag aaatatcaac gtccggttca ctcaaaccaa cgtttggtga
     1141 tttattgact cttcaaaaat acatgatagc tttgtcaaca acttataaag ctaaccataa
     1201 agcgcttgat ttcattaaaa aaaatttcac aactattgac gatattgatt ttagtactaa
     1261 aaaaataatt gatgatttat acgatacaag tgatacaatg gatagaatca ttttaagtta
     1321 cagccaatat ttggacaaat tggaaaatat gataaacaat atgatttcgt atcagctaaa
     1381 catgattatg aaaaccctaa ccgagatatc aattgttttg actattccag caattatttt
     1441 tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag tcttcttatg gtgttttagc
     1501 cgtattgatt atctctgcta tactaagtct aatttgctgg cttttgatgc gacgcaaaac
     1561 atatttgtaa agtatttgtg ataccctaat atgatttaaa gcagaatttc tttgttgact
     1621 ttttaaaagg ctgattcctt tcaataattt aatagcttca gcggctgctc agaagtcaat
     1681 aaattggact atttttattc agttacttag gcaacatttg agatacgatt gcgattataa
     1741 aaattgatat accaaccaat ggctgcttgg aagactgaca tattagcctc gatatcatcg
     1801 aataactaaa tacagaaaaa tacaataaat attatcaaac tagggactat actagaaaac
     1861 atcagtacat tccttaactc agggtctata attagaaaat cgcccctttg aaaactgatt
     1921 aaaacagccc ccattatcta gtgactagat aatgggggct gtttttttgt taagtgatat
     1981 aataaaccat aaaagaagtt tagaaatacg gtcctggtca tcaataaata aacaatttaa
     2041 ttaattattt atttaattat ttaatttcag aaaggtatac atgaatcatg gtgcaacaaa
     2101 ttgtcctacc catcaaagac tccagcgtct taaagatggt acaagataca cttctcgata
     2161 gtttccgtgc tggtcgccga aattacaccg tttttcaagt tggtaaggcc acgctactac
     2221 gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta taatccagac ggctctgtca
     2281 aaaatgcaac ctttattcat gataaaaaga ccggtaaagc aaacacgtta tatttaaagc
     2341 ctgttcagca agacttgttg caatatcatg attggctggc ccaagaaaat atcaattctg
     2401 agtggttatt tccctcgaca gcccattatg atcttcatat cactgagaag cagtttcata
     2461 aggtgatggc gcatgttggt gatctcttag gaatcaactt ggaagacaaa gagaaaaaat
     2521 tgcgagtcag aaaaccgttt tgagatatga acagcatttg ccttcacgcc ttagcgattt
     2581 taaataatag ccttgaaagt gattatggaa aagccctcta cgctcaatgc aaaattgacg
     2641 tagaaactat ttttgatcga ttgaaaggtg tatttggtat gtgaagaacc catgtcagag
     2701 gcaagaacgt tgcacctact gacattggaa tgatgcttat gagcatgaat ctaaccaaat
     2761 tatcccttga ggctagaaga aaagcggaag cttttcaaca caaagcagcc aaaaataaaa
     2821 aacgcgacga agcaatcaca tttatgatta ttcatcgcgt tttttatatt tagagctagt
     2881 tatttgctag acccactatt tacagatgaa acgtttatca cctttggttt tgggcattta
     2941 gtggaattag cagaaccagg tcattatgac gaaaagtggc aaaattggaa acttgaatct
     3001 ttgccgattt ttcctgatcg atacgatttt gaagttgcaa aagataaggg aaagcagttt
     3061 aaaattgttg cagaacttct caaaaaggca aatacaatta ttgttgcaac agatagcgac
     3121 agagaaggtg aaaatatcgc ctggtcgatt atccataatg caaatgcctt ttcaaaagat
     3181 aaaacattta aaagactatg gatcaatagc ttagaaaaag atgtgatccg aagcggtttt
     3241 caaaatttgc aaccaggaat gaattactat cccttttatc aagaagcgca aacacgccaa
     3301 attgccgatt ggttgatcgg catgaacgca agccctttgt atacgttaaa tttacaacag
     3361 aagggcgtac aaggtacatt ttcactagga cgtgttcaaa cgcccacctt ataccttatt
     3421 tttcagcgcc aggaagccat agagaatttt aaaaaagaac cttttttcga ggtggaagct
     3481 aagtaaaaca agggtcattt aagggcgttc taagccccac acagcgtttt aaaacccaag
     3541 aggagctttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca agaggggata
     3601 attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagttcgtt ttctttaagt
     3661 agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac tttaaaagct
     3721 atgcaaggac tgtatgaagc aaaattattg agttatccaa gaacagatac accatttatt
     3781 acagagaacg aatttgctta tttaaaagcg aattttggca aatatagcgg ttttttagga
     3841 cttgatcttg aaatgttgtt atcattgata atgatacgtt caaacaacag cacccaaatt
     3901 ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa acatgcaaca tcctattcta
     3961 atcagctggt aaagcttaat taggttatat aagcttgaaa ataaagggtt tgtccaatat
     4021 ggacaagcct ttttcttatg ttttgccaca gatttgccac acttattgtg taagagtatc
     4081 aaggataggt ataatttgat actttgtttt tttagtgatg tgcaaataaa tttctgttac
     4141 acgactacct ctagaatgcc ctacacggtc ttgaattgct tctaggggta ttccttactc
     4201 tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc gtaatatgtt tattaaaact
     4261 aagtttatta tcaatatcaa ttcctgctcg ttttggatta ccttttcgat ttaattttcc
     4321 attttcaggt gagttgagga aattgttaag gacactataa tgaagaggtg ttccattata
     4381 gtcgggttta tgacgacttt tggaagtaaa aatccatcca ttatctttat aatcaggatt
     4441 gaatctcata tgttggtgat ttaactcaat ttcctcttta agaatttcta tagccctttt
     4501 agataattgg acatttcggt aactttgtaa gttttttaca ttaccttcat agctattttc
     4561 tgcagctcct ccgtgagtgg actgactaat gatgatattt tgcttttcaa aatattgata
     4621 ggcaccaata ttttggagtt tcgactctgt aagtcaacag ggccattagt tagaatacct
     4681 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
     4741 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
     4801 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
     4861 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaatatct gaaacgtttg
     4921 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
     4981 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
     5041 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
     5101 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
     5161 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
     5221 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
     5281 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     5341 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     5401 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     5461 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc caggtccaat
     5521 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     5581 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     5641 caattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     5701 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     5761 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     5821 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     5881 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     5941 ataagaacag aggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     6001 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     6061 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     6121 gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa ataaacaaaa aaagtgttgc
     6181 tttttgtttg aatgcttgat attatataaa caaagaaatg atgaaaacgt tttttcgaac
     6241 attttgcaaa atgttttcta cttctacgta gcatttcttt ctaaaattta ggaggtggtc
     6301 aaaatggcta ttgttgttgg tgcagatctc aaaggtacga gattaaaaga tgttgtgaaa
     6361 aattttcttg tagaagaagg ttttgaggta attgatgtga ctaaggacgg acaagatttt
     6421 gttgatgtta ccttggcagt tgcctctgaa gtaaataaag atgagcaaaa cttaggtatt
     6481 gtaattgatg cttatggagc tggtccattc atggtagcaa ctaaaattaa aggtatggtt
     6541 gcagcagaag tttcagatga acgctcagct tatatgacac gtggacataa caattcacga
     6601 atgattacgg ttggcgctga aatcgtcggt gatgagcttg ctaaaaacat cgctaaaggt
     6661 ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc gagtagatat gcttaacaaa
     6721 atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt ggatgtgacc acattgtcac
     6781 agatgaaaaa atggcagtat cagaattctt gaaatcaaaa ggacatgagg tccttgattt
     6841 cgggacatat gaccacgcac gtactcacta ccctatctat ggtaaaaaag tcggtgaagc
     6901 agtagtaagt ggtcaagcag acttgggagt atgtatctgt ggtacaggtg tcggcattaa
     6961 caatgcagtc aataaagttc caggtgttcg ctcagcgttg gttcgtgata tgacatcagc
     7021 tatatatgca aaagaagagt taaatgcgaa tgttatcggt ttcggtggaa agattactgg
     7081 tggtcttctt atgaatgaca tcattgaagc tttcattgaa gctgagtaca aaccaacaga
     7141 agaaaataaa aaattgattg caaaaatcga acacgttgaa acacataatg cacatcaagc
     7201 agatgaagat ttctttacag aattccttga aagatgggac cgtggtgact accacgatta
     7261 aggtataaca aatgattctg acagtcacac taaatccttc agtagacatc tcttatcctc
     7321 tagaaacatt gaaaattgat acagtgaata gggtgaaaga tgttagtaag acagctggtg
     7381 gaaaaggctt aaatgttact agagtcttgt atgaatctgg tgataaagtt actgctacag
     7441 gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga attagaacaa tctcctgtta
     7501 gtccagcttt ttataaaatc tcaggaaaca ctagaaactg tattgctatc ttacacgagg
     7561 gtaaccaaac agaaattttg gaacaaggac caacaatttc tcatgaagaa gcagaaggct
     7621 ttcttgacca ctatagcaat cttattaaac aatcagaagt tgtgacaatt tcaggaagtt
     7681 taccttcagg acttcccaac gattattatg aaaaacttat ccaacttgct tcggatgaag
     7741 gagtagcagt cgttttggat tgttcaggtg cacctcttga aacagttcta aaatcgtcgg
     7801 ctaaacctac tgccatcaaa cctaataatg aggaactttc acagttgctt ggaaaagaag
     7861 taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga gtcacttttt agtggtatcg
     7921 aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc aaaacatggt gatgttttct
     7981 ataaggtaga tattcctgat atcccagttg ttaatccagt cggatcaggg gactcaacgg
     8041 tagctggtat tgcatcagct ttaaatagta aaaaaagtga cgctgactta ttaaaacatg
     8101 cgatgacatt gggtatgtta aatgctcaag aaacaatgac agggcatgtg aatatgacta
     8161 attacgaaac actaaactca caaattggag taaaagaggt ataaaaatgg tacttacaga
     8221 acagaaacgc aaatctttgg aaaaaatttc agataaaaac ggtgttatct cagctttggc
     8281 atttgaccaa cgtggtgctt tgaaacgttt gatggcacag tatcaagata cagaaccaac
     8341 tgtagctcaa atggaagaac taaaggtttt ggttgctgat gaacttacaa aatacgcttc
     8401 ttcaatgttg cttgaccctg aatatggact tccagcaaca aaagcattgg ataaagaagc
     8461 aggtcttctt cttgcctatg aaaaaacagg ttatgataca tcaagtacaa aacgtttgcc
     8521 tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag caaggcgctg atgccgttaa
     8581 gttcttgctt tactatgatg tagacagctc agatgaactt aaccaacaaa aacaagctta
     8641 tattgaacgt gttggttctg aatgtgtcgc tgaagatatt cctttcttct tggaaatcct
     8701 tgcttatgat gaagaaatct cagatgcagg ttctgttgaa tatgcaaaag tgaaacctcg
     8761 caaagtcatt gaagcgatga aagttttctc tgatccacgc tttaacattg atgttcttaa
     8821 agtagaagta ccagttaacg ttaaatatgt cgaaggtttt gctgatggtg aagtagttta
     8881 cagtaaagct gaagctgctg acttctttaa agcacaagaa gaagcgacta accttccata
     8941 catctaccta agtgcaggtg tatctgctaa attgttccaa gaaacattgc aatttgctca
     9001 cgactcaggt gccaagttta atggtgtgct ttgtggacgt gctacttggg caggatctgt
     9061 agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa tggttgcgta ctactggatt
     9121 cgaaaatatt gatgaactta acaaagttct tgttaaaaca gctagtccat ggactgataa
     9181 agtatagtaa aaacataaaa cggaggatat tgttatgaac agagaagaga tgactctctt
     9241 agggtttgaa attgttgctt atgctggaga tgctcgctct aaacttttag aagcgcttaa
     9301 agcggctgaa aatggtgatt tcgctaaggc agatagtctt gtagtagaag caggaagctg
     9361 tattgcagag gctcacaatt ctcagacagc tatgttggct cgagaagctt ctggggagga
     9421 acttccatac agcgttacca tgatgcatgg tcaggaccac ttaatgacta ctgtcttatt
     9481 aaaagatgtg attcatcacc tcatcgaact ttataaaaga ggagcaaaat aatgcataaa
     9541 ctcattgaac ttattgagaa agggaaaccg ttctttgaga aaatctctcg aaacatctat
     9601 cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag tcatcctttt ctcatctatc
     9661 tttatcctta ttgcctatgt cccaaatgct tggggattcc actggtcaaa agacattgag
     9721 accttcttga tgactcctta tagctattcg atgggtatcc tagcattctt tgttggtggt
     9781 actaccgcta aagctttgac ggactcaatg aaccgtgacc tacctgcgac caatcagatt
     9841 aacttcttat ctacgatgct agcgtctatg gtagggttcc ttttgatggc agctgagcct
     9901 gcaaaagaag gaggcttctt gacagccttc acgggaacaa aaggtctttt gacagcattt
     9961 attgcagcct ttgtgacagt taatgtttac aaggtctgtg tgaaaaataa tgttactatt
    10021 cgtatgcctg aagaggttcc accaaatatc tctcaagtat ttaaagactt gattccattc
    10081 actgtagcgg ttgttctcct ttatggattt gaactcattg ttaagggaac tcttggtgta
    10141 accgttgcag aatcaatcgg tacccttctt gctcctctct tctcagctgc agatggttac
    10201 cttggaatta cacttatctt tggtgcttat gccttcttct ggtttgttgg tatccacggt
    10261 ccttcaattg tcgaaccagc aatcgctgct atcacttatg ccaatatcga tgccaacttg
    10321 catcttatcc aagctggaca acatgcagac aaagttatca cttctggtac tcaaatgttt
    10381 attgttacca tgggtggaac aggagctaca ttgattgttc cattcttgtt catgtggatt
    10441 tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag ttgttccaac attctttggg
    10501 gttaatgagc caatcttatt tggtgcacca atcgtattga atccgatttt ctttgtaccg
    10561 ttcattttcg ctccgattgt caacgtttgg atctttaaat tctttgttga cacattgaac
    10621 atgaactcat tctctgccaa ccttccatgg gttactcctg gtccgttggg aattgtactc
    10681 ggtactaact tccaagttct atcatttatc ttagccggac tcttggtagt tgttgatact
    10741 atcatttatt acccatttgt taaggtatac gatgaacaaa ttcttgaaga agaacgttct
    10801 ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa acttcaatac tgctaaagca
    10861 gatgccgttc ttggaaaagc aggcgttgct aaagaagatg tagcagcaaa caataatatc
    10921 acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag gtacaagtgg cttgcttgcc
    10981 aatgccttga acaaagcagc agcagaatac aatgttccag tcaaagcggc agcgggtagt
    11041 tacggtgctc accgtgaaat gttgccagag tttgacttgg ttatcttggc tccacaggtc
    11101 gcttcaaact tcgatgatat gaaggctgaa acggataaat tgggcattaa acttgcaaaa
    11161 actgaaggag ctcaatatat caaattgaca cgtgatggac aaggtgctct tgcatttgtt
    11221 caacaacagt ttgattaata aatttgtaaa agttatttga gacgagatta ttctttcaat
    11281 tccttataat attgttaacc atatcattgc atttcatttc cttggtttcc ggaactcaga
    11341 atatcgtctc aaatgctttt tttgaaagga tttatagtta tgactaaaac acttcctaaa
    11401 gattttattt ttggtggtgc tacagctgct tatcaagcag aaggtgcgac tcacacggat
    11461 ggtaaaggcc cagttgcatg ggataagtat cttgaagaca attattggta cacagcagag
    11521 cctgcaagtg atttttacca taaatatcct gttgatttag aattagctga aaaatatggc
    11581 gttaatggta ttcgtatttc aattgcctgg tctcgtattt tcccaactgg ctatggcgaa
    11641 gtaaatgaaa aaggtgttga attctaccat aatctttttg ccgagtgcca caaacgtcat
    11701 gttgagccat tcgtgacttt gcaccacttt gacacaccag aagttcttca ctcagatgga
    11761 gatttcttaa accgcgaaaa tatagaacac tttgtagatt atgccgcttt ctgttttgaa
    11821 gagttcccag aagtaaacta ctggacaact ttcaatgaaa ttggcccaat tggagatggt
    11881 caatacttgg ttggtaaatt ccctccaggc attcaatatg atcttgccaa agtcttccaa
    11941 tcacaccaca atatgatggt ttctcatgca cgtgctgtga aattgtataa agataaaggt
    12001 tataaaggcg aaattggtgt agtacatgcg ttgccaacca aataccctta tgatccagaa
    12061 aatccagcag atgttcgtgc cgctgagtta gaagatatca tccataacaa atttatcttg
    12121 gatgcaacct accttggaca ctattcagat aaaacgatgg aaggggtcaa ccacatccta
    12181 gctgaaaatg gtggagaact tgatcttcgt gatgaagact tccaagcact tgaagcagct
    12241 aaagatttga acgatttcct tggtatcaac tactacatga gtgattggat gcaagccttt
    12301 gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa aaggaagttc taagtaccag
    12361 attaaaggtg ttggtcgccg aatcgcacca gattatgtgc ctagaacgga ttgggactgg
    12421 attatttatc ctgaaggctt gtatgaccaa atcatgcgag tgaaaaatga ttatccaaac
    12481 tacaagaaga tttacatcac tgaaaacggt cttggataca aagatgagtt tgtagataat
    12541 accgtttatg atgatggtcg tattgattac gtgaagaaac acttggaagt tttatcagac
    12601 gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct ggtcacttat ggatgtcttc
    12661 tcatggtcaa acggatacga aaaacgttat ggactattct atgtagactt tgacacgcaa
    12721 gaacgctatc ctaagaaatc agctcattgg tataagaaag tagcagaaac tcaagtgata
    12781 gagtaatatt acttgaaaat gtaaaaaatt gttatccgat actttagaaa aagtacaaca
    12841 cttatttaga ccaggctttg aaaaggtata tattgttagt ttcgaagatt gtcctatgat
    12901 tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa tggtatgttt caactggaaa
    12961 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
    13021 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
    13081 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
    13141 tttaaaacac ttggtggtca actgacttcg attaaagaca aggatgggat agagtatctc
    13201 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
    13261 agtttacgaa atgattgggc tatttataga cctcaagaaa gacccttttt tacaggtctt
    13321 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
    13381 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
    13441 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
    13501 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
    13561 cctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
    13621 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
    13681 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
    13741 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
    13801 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
    13861 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
    13921 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
    13981 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
    14041 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
    14101 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
    14161 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
    14221 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
    14281 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    14341 gt
//
LOCUS       DASJPC010000064        14258 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJPC010000064 DASJPC010000000
VERSION     DASJPC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.064"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(72..377)
                     /locus_tag="U4A13_001950"
     CDS             complement(72..377)
                     /locus_tag="U4A13_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780228.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            complement(559..990)
                     /locus_tag="U4A13_001951"
     CDS             complement(559..990)
                     /locus_tag="U4A13_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN1780229.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            complement(1005..1301)
                     /locus_tag="U4A13_001952"
     CDS             complement(1005..1301)
                     /locus_tag="U4A13_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780230.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            complement(1378..1797)
                     /gene="ssb"
                     /locus_tag="U4A13_001953"
     CDS             complement(1378..1797)
                     /gene="ssb"
                     /locus_tag="U4A13_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1780231.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            complement(1852..2193)
                     /locus_tag="U4A13_001954"
     CDS             complement(1852..2193)
                     /locus_tag="U4A13_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780232.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            complement(2265..2531)
                     /locus_tag="U4A13_001955"
     CDS             complement(2265..2531)
                     /locus_tag="U4A13_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780233.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            complement(3018..3929)
                     /locus_tag="U4A13_001956"
     CDS             complement(3018..3929)
                     /locus_tag="U4A13_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEN1780234.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            complement(4872..5294)
                     /locus_tag="U4A13_001957"
     CDS             complement(4872..5294)
                     /locus_tag="U4A13_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1780235.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(5284..7458)
                     /locus_tag="U4A13_001958"
     CDS             complement(5284..7458)
                     /locus_tag="U4A13_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEN1780236.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(7474..9816)
                     /locus_tag="U4A13_001959"
     CDS             complement(7474..9816)
                     /locus_tag="U4A13_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780237.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(9838..12381)
                     /locus_tag="U4A13_001960"
     CDS             complement(9838..12381)
                     /locus_tag="U4A13_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1780238.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(12391..12789)
                     /locus_tag="U4A13_001961"
     CDS             complement(12391..12789)
                     /locus_tag="U4A13_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780239.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(12870..13154)
                     /locus_tag="U4A13_001962"
     CDS             complement(12870..13154)
                     /locus_tag="U4A13_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780240.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(13178..14176)
                     /locus_tag="U4A13_001963"
     CDS             complement(13178..14176)
                     /locus_tag="U4A13_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780241.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         3987 a         3035 c         2219 g         5017 t
ORIGIN      
        1 tgttgaaaca atagcacatg ttgccctccg tagttttctg tagaggattt ttttattctc
       61 cgctaaagta tttaaaaata tcgtatattc agttctgcta gttgctctat ttcttctgta
      121 aaacgttttg tcgcattccc actctcatct gaatcaatca aacatgtttt accaactctt
      181 gacagataca ccgctagtct tccatctcga atttcatgac caacattctt acttttcgat
      241 aatgttcttt gcattcgcaa tcttatatct ttcgaatata agtcggttat agacccgtac
      301 attcgcaagt tcgtcagttt aattaaaaac agtctcaccc tgagtaattt atagcgtccc
      361 ggcatatcca acttcatatg attccacact tagaaattgt tctgccgcct ttacctagct
      421 tcatacaata acttcctagt agactttatt gcatgtagga gtatcagacc agttgattca
      481 gcagacatga tagctttcat tccaactgtc aaataggtta gttgtgatta atcttaagat
      541 taatcctccc gtttcgtatt acatacttat ggggaaactt gtcgcacgtt gatagccaat
      601 caaatgacaa cgattataaa gataatcgcc attcttcatt ttcttttgtt tccagccagt
      661 tggcttaatg tataatcggt ctcgttcttc tgttggcatc ctgcttttat gcaaaagtgc
      721 acttgctaca cctacccgat ttaaacgatc caaatctgaa aaaaattgcc tggttccatt
      781 ttctaacgaa aaatcttctt cagaaaaact cggtttattt tgattcacta tttctacttg
      841 tgtatttagc ggttgttctt catcttgttt tgttattgct tgttttattt ccgataaggg
      901 agctttttga acgtcaattg cttcacaacc tgtcccaaca aaatcaatca atgctagcac
      961 aataaaaata cttcgtttta atcttttcat tttttctctc ctctttaatc attgttttct
     1021 tgttcatatc gattgaacaa ttgaatcgca tttatgggtt cttctcctgt ttgtttcgca
     1081 atcaatttag acaaaatttt ttccaaccat tttttgtctc cgacataatc taaaaagttg
     1141 cccatcgttg ttaataccaa ttcatcactt gcaaaatcta ccaagcgaac gctttgataa
     1201 ggaggtaaac ttgcaacata attaataata ttttcatctg tataatcaaa cgagcccaaa
     1261 tattgcatgg ttcgttgatc gtaactgtca atgattttca taaaaaatcg cctcatctct
     1321 atttattttt tgttctatac ctgaagaaat caggtataga acaaaattat ttttcactta
     1381 aaagggcaaa taactatctg aaatttgaat ctcatttcct ggaattgaag aattattttg
     1441 attattcgcc attgattttg accgttgctc attgatttct ttactctcta acaaatagaa
     1501 atctgtcact agaacttccg taacatacat ggtctgtcct tgctgattct gatagctcct
     1561 cgtttgaaga cgtccattaa tgccaatcaa acttcctttt ccagtatatt ttgctaaagt
     1621 ttccgcagct ttccgccaaa tgacacagcg gatataatct gcttcacgct cgccgtttgc
     1681 attcgtataa tttcgattaa cagctaaaat aaatgtgcct gtcgcattgc cttttggtgt
     1741 gtattttaaa tccacttctt ttgttaaccg tcctactaaa ttcactgaat tcatcatgat
     1801 tttttctctc cattcttcta tttattattt ttgaaagggg aaactatccc cttaataata
     1861 actactttca taaacagctt gatttttata tctatgaaac gtgtcccaac tacgataacc
     1921 acataacata tcaggatagg tttctggatc aattcgtttt tttacaatac tgtctttagt
     1981 ttttgctaaa ggatactcta taaacttgtc tttcattgga acaacttttc cactcatgga
     2041 cgaataatgc tcgaccgttt tcttttgaat aggctgaaac catgctgttt tttctgttaa
     2101 cttcacaatt tgccaaaaag tcacgttcgt ttgttcgtag ccccaactac tataaaaaat
     2161 gtctcctact tgataaggtg tgttttttgc cattttcttt ttccttcttt ctttatattt
     2221 ttaccgtaca aagagaatca ttctctttgc acgtcatctg acttttataa gaagttttca
     2281 agcttcccgt tgttaatttc ccactcatac cattcagtga aatcttgcgg aaaaccattc
     2341 actgcatgtt tttcttgttc ttttacttct tgttcgttca ttgggtctaa ggctgtaggt
     2401 ttgtttatcg gtcttgcata acaaccaata taggtttcaa aatctccttc aaagctcccc
     2461 tcaataatca aacctctttc ttgcaactct tttcttgcct gttctttcag ttgctcgttt
     2521 ttagttgtca tggttgtttc ttcctttctt gaatagaatg ttttatttga ttttttcgcc
     2581 acccaccccc accttttcgg tagtgtgtcg ggggcacggg ggtttggggg tgcaaccccc
     2641 aaggtcttca ttcggctcgt ttttagacga aggcctgctt gtttgatttt gggtttcagt
     2701 tgtcaaggat caaagtggac ttctgtttag aggccctttt cgtatagcgg tcgttcggca
     2761 taaccggctc gaaaacatgt tctgacgatt gccaagggcg cgcagcgtgc attttaccct
     2821 tggcaattgg caggttatgt tttaggttat actcgccgaa tgactgctat acgattaggg
     2881 actctcgaac gtccacttct ttgacaacgg ataaatcaaa caagttagtc cttcctaaaa
     2941 acgagccggt tcagacgctt gcgtctgaac ggtgccagca aactggcaca aaaaaagagc
     3001 gaagcgaaca ccctctttta caatacttca ggcgtaaact tcacttttgg tttttcctca
     3061 ttttcagttg attcttcaaa taaattcaaa cgactttgaa cagcgccatt cggataaatt
     3121 ttttctcgtt ctactgtccg ccaattgact gctggatcat tctctaataa tgcttttaat
     3181 ccctcttcaa actctaaagc tgaaaaaata tgcaaccgct caaacaattc ttgttgactc
     3241 gcatcttttt cagtttcgcg cttgaaaaaa atacgcttaa acgcatgaag ttcttcttga
     3301 attgcgccac taggtgtcac ataaattaaa tgtgattcag gacgtgttcg caactcacga
     3361 atatatttcg gaaaaatcgc ttcacggtag cgacgacgtg ttttcaacgt tagctcaatt
     3421 tcaaaggacc aattgatctc atgattttct ttgtctcgaa aacgaaaaat aaaatctggc
     3481 acaaaaaatt ttcttccttt tgtcttagga tttaaatcct tcgtttcata gacggtacgt
     3541 ctttctgaaa taatccgttt taaatgtggg tggtcttttg ccaatgccat tcctttaatc
     3601 aaataattgt taatttgtaa atggtgattc aaattatatt caggaacttt cggcaacgta
     3661 taatacccac ctatgctttg atgtccctct ttcgtcaaat aataatacat ttctgttcca
     3721 tatttttttc gatcgattaa ctgtttttca tataatctca ctaaagcatt cgttactcct
     3781 tgtttactaa ttgatctgtc aaaattcaat agtttgtata gctgttttct agtaaccact
     3841 ctcaattcat gaagcaaaca tagaatcgct aaagtactac tattgcgagt agaataatca
     3901 ataaaatagc catgcaataa atacttcata tttcatcacc ttcaatttaa taatgctttc
     3961 ttgcaagttt ctgttcgaac aaatcttcct gattcctctg caactgcttt actcccctct
     4021 cttgtaagtt tgtgttcgaa caagtcttca agtttcctct acacttgttt tactccaact
     4081 ctctcgcaag tttctgttcg aacaagtctt caagtttcct ctacacttgt tttactccgg
     4141 ctctctcgca agtttctgtt cgaacaagtc ttcaagtttc ctctacactt gttttacccc
     4201 cactctctcg caagtttctg ttctggacag ccccaagaac actggggcgt tggccgtggg
     4261 gttgattctc cccctctttc aaaagaggct gaaagagggg gttagggggg agttagtttt
     4321 gagaccccaa gactggggta ggggttgatt ttttcccttt taaattttat gctactacta
     4381 tttttagtag tgtattcttt atctgttggc tgtcggcgct tggtatgtta ttcggagact
     4441 tcccgcttag gtttttgacc attttctctc ataagtggcg gtttttccac gttgagagaa
     4501 aatggtcaaa aacctggaag tcggagaatg cctttcatac caagcgccga cagccctccc
     4561 cccacttatt acatataacc tttaaataat ctctaaataa ccctcaaata atccttacta
     4621 atactgaaac gtaaaaaaaa taaacggatt tcaaacaaaa tacgtttatt ttttctattg
     4681 attttagctc aaaatcgatt caattcgagt gaaatttgct tcaattttca attggttttt
     4741 gttcaaattt tgaaacattt ttgctcaaaa aatgagtgaa tttgtttcaa aagaaaaaca
     4801 aatacgatcg aaaaaaattt gaactcataa caattttttg tttttttcaa cgtattctct
     4861 ttcaattttc actaaaaatt aggaagaaat tgattcaatg tcttttcaaa aaagaatgtt
     4921 tttctctcaa tttttttgac gtttctatca tgaatcacga gaaaacttgg gacactttta
     4981 acatggtaac gttgtaaaaa agtttgaagt tcttcttgtc ttggttcttt tcttgtttca
     5041 attcgatata cattcctaga gaattttttg tcgtatcttt ccaagaaaga ttcaacttct
     5101 tggcattttt tacaatcatt tcttgctaga tacacaaaaa atgattgatt cgttttttga
     5161 agttctagta cttcttctaa attactattg attagttttc catttgattc ttcgttagtt
     5221 tttatcagtt taaaacaacc aaataagagg aaactaacaa taagtatcct aacaataact
     5281 ttcttatttc gcatattgtt tctccaaatt tggtttccga atatagacga ctcgcggtgg
     5341 ttcctggacg tcttcagctg ttcgatacac cacaaattcg cctgttctca atgctcgaat
     5401 gcgattcgga ttgaatttaa agcggtctac gtctcgtttc gtgcctcttt ctcctgtcca
     5461 atcaaccttt cccatatcag aataacctgc ttcttgtgtc gtcatttccg ttaattcaat
     5521 gtcttcttct gtccccatta aatctgccca ataatcaatt tctgtcgggt catttgtctt
     5581 cccaataaag aacgtattca cattcccaat caactgtttt cgaaggacag gatcaattct
     5641 atcaatgtcc gcaagcgttt gaggggcata cgccgcatgc accccagcgc ctcgtccctt
     5701 attgactaag tctataaggt tatcatttag ataagaagct ggttcgtcaa atattactag
     5761 gactttcgag cgattggctg tttctcctgc ttgtttatac acttctgacg caaagaaatt
     5821 aatatctccc acaagcattt gagccaatgt ccgaataaac tcactataaa tcaatccgtt
     5881 taacgagata taaacaatct ttccttcttt aacaacttct tgaatatcta attctctttt
     5941 tcctccagaa gtatcaaaga gttcaccaag ctcactatcc aataacatat tgatattggt
     6001 ctgtaaattc tcagaagtcg catacaaata aaacgggttc ttcttatgtt cgtaacgaac
     6061 aaacaaccgt ttaaacaacg tttgctcgat ttctgtcatg agagaaatgt tttcttttaa
     6121 taatagataa tacgtttcta agtcaacggt tttacgatca tgttcaacga ttcgctcttc
     6181 acttaactgt tccacatcgg caaaatcacg ttctgttttg tccatcgctt cattcttttt
     6241 cgtttttttc ttcgtggttt tcttcttttc tacgaccact ttttctttaa ataaatctgg
     6301 atttttcaac tgaatacgat ccgcaaacaa atgtaagacg tttcgtggca ataaataatg
     6361 ttgtaaccaa actagatttc tttcaatttt atggctcttt tggaactcgt ctaaaagctg
     6421 aatcgttacc tgtaataacg ctttggctgc tgtcgaataa tacacgcttt ctgtttctgc
     6481 tagattggtt aatttatctc gaatggcaat atcattgcca ttttttacag gattataacg
     6541 caaatcgcca ccgtccacaa agacttcaat cgggcgattg tatgcctctg ccacttcttt
     6601 taacgcttgt aaagtctctc ctgctccttt cccatcgact aagataacgg ggatacgaaa
     6661 cttacacgca tgttctgcta gtaattggat caccgtcgtt tttcctgaac ctgtagtcgc
     6721 tggaataatc acatgttggt tcaattcact aaagttcatc acataaggga gcccatactc
     6781 atctgttcct aatagaatca ctttggctaa tgaagccagt tgtttattcc gcaaagcaac
     6841 gtcttttatg acaattgcta cctggtacgc ttgctgatac tctgctgtat atttttggga
     6901 ctcctgaaat ttattcttcc gacgttcctt gtattcttta ctatttagca aagctgtttg
     6961 ccgttctttc tttgaaacaa cttttctttt ttcaataaaa gacgcatacg caatcaaaca
     7021 acttcctgca cctgtcccac ctaaaaggag ggcaaaaatc gtttgtccat taaactgcaa
     7081 cgcatgagaa aacaagttta atggaaataa taagtaacac gacaaatacc ctctaggaag
     7141 aacaaaaata aaacaaaata gatttaatag cgtaaaacct aacgcaaaaa taaaaaaaag
     7201 agagcttctt cgttttaaga agctctcata ctcaaagtca taaatatgtg cacgtgtctc
     7261 ttttttatct atcataaaga caatcgctaa aggaatactg cctacaataa aaaatggata
     7321 tagacaaaca cctaataaag tgacgataaa ataaagttgt tgaaaccgac tttttggcgt
     7381 ttctttttct tgttttcttt tgcgatcgac agaaaacatt tgctcaaaat gtgtcggtcg
     7441 ttttggtgca ttcatcatac tatcgatttc ctcctatcgt tttcgttgtt tcatttctct
     7501 tcgataaatc ggttgttttt ctcgttttcg tttcattttt gcttgagttg ctcgaaactc
     7561 actccgtgtc ttcggtgctt gttttggtga ttttgtttgt ttcatggttg agtatgcttt
     7621 agatacggtt gaaggcgctt tggtttgtgc tgttactcga ttgactgctg acatatgttc
     7681 ccgaaccccc tctggacgtc gaacagattt cggtgtggta gaacctgttg ctcgtggtac
     7741 attccctttt cttagtggtt tttgtaccgc atgtttttgt cctgcaactg tccgggtttt
     7801 catcgaactt gtttttgtta cgggagtaag tgcctccttc cgacgacgac ttgtttgtcc
     7861 ctgacgacgt tttcgatcgt tcaattctgt tctaggcgta tgcttctggc cataagactt
     7921 gtgtttttgc tttggattcg ctaatgacac caccattgca gaaccctgat tgcgatacgg
     7981 actacgagac gcaccttttc tacttctttc tcttagacta ggattgcctt gcaattctcg
     8041 ttgtaataaa tcggctgttt ttcttgcctg tttctccatt gctgttcgtt gcatacgttc
     8101 ttgactttgc tcttctgctt gtttcgcttg atcatgcaag tctcttgctt tttgtgccat
     8161 acgttccata cgatttggtg ctttatgacg aggttgttgt gcggattcac gccgaaaagc
     8221 acttcgtgtg gcttgtttcg ccgtttgtgg catacgaacg tctgagccac ccccacgttt
     8281 tcgttgacgt tcttgtctct tccgtgcgtt ttctttttct ttttgtttct gtgcttttct
     8341 cgcttctttg cgttctttca accgttgttt ggcttccctt ctcaattttg cttctgcccc
     8401 aaatggattt cccgcaaaag ctaacgctcc tcgaacaccc attcttccag taaatagact
     8461 gcctaaaaag cctcggaaca aataaattcc caacggcgca aacaaataaa caatgatcaa
     8521 gagaattttt tcccatggat cgtcttgtgt actacttaat tggaacccgt aactaagaaa
     8581 agaagaaaaa aggatacatg caaaacctgt cattcctttt aaaaacgtca aaataccaaa
     8641 aactttggca tagttaccaa acacattcat tgtgacaaga aacaatccta aaaaaagtaa
     8701 gaacggcaac atcggtacta acaatagttg catagttcca ataatcaatc gaaacatact
     8761 taagacaaaa taaacaacgg tttgaatcag attcacgaca aaataaaaca aggcaaatgc
     8821 tgtatttttc caactattgt aatattgtat gttcttgttt ttcagtgtat ctgcttcata
     8881 ctctgtgact ttgtcatgaa ttttctcccc tatttttgta cctaaatcat tgtccaataa
     8941 aagattgatt cgatcatatt cttttccagc atatttgact ttcttctcac gaattgtgtc
     9001 tacattggtt gtgccataat ttaacaacag atatggttca taaacattac tataaaagac
     9061 ccgagccgcc atcaatttgc ctgccgtttc cactttttta tccgctgtta cctcttctgt
     9121 ttcttttgtt tcataatttg tttcttcttc aggtgttaac actggattga ccttcacgaa
     9181 cgccgtttcc actgtagaat ctaaagaaaa aagaacatca aaaagattct tatttgtccc
     9241 agaatctttt aatacgctta acccagtaaa aataaggatc gtcataagaa agacacggag
     9301 tgcttgtttc attcgagcct ctccagcata tttgattgcc atcataattg ccgaaaaact
     9361 gattccaata gttcctgcaa tacttaacat attgtccgca attttttgtg ttaagttttg
     9421 aatgggttct cgaatggcgg aaataatatc taattcaaag agaaattcaa ccattgaagc
     9481 attgaattct cctaacattt ttacgccact ccaaacaaaa tttttcatca ctcctgcaac
     9541 attgtacgat agcttttttt taaggttcat tgactcatag tcattggtca tcaattcaaa
     9601 cgaattcttt ttgtaatttt tatacaattg atccacatca gtcgttactt cttttacttc
     9661 tactttatcg ttggcattct ctccttcaga acgatcggtt tcagtgacac ccgcaccatc
     9721 attcatttga tcggcagaaa cagtagcccc ttgtagacag aaaaaaagac cccctaataa
     9781 aaggagcgtc caccatttca gagatttttt tcgcaaaaaa accacctctt ttttgtttta
     9841 tcgttgtttt tcattttcta acattgcttg aattttttca tcttgatttt cagtagaaga
     9901 aacagccaat aaccaactat caaacaacac atcaatggca atcggttgat ttcttcccaa
     9961 atgatcctgg aataaacagg ttcctgtttg taattgttcc atcatttcta aattggctga
    10021 attcacttcc atatcaaaga aggacaatag tttctctcgt gcttcttttt gtttcggacg
    10081 aaacgcaaat ttgtaagaaa ttaactcttt tttatcttct tgatcgtaat ctttaaacgc
    10141 ttgcacaaca aggtaaatat ccgtattatt cgcacgtcct tttcgcaagc tatcatcaat
    10201 caaatagctt ccttgtgcgg tatcttcata cccttttgcc tcgtcataaa taatcgcagc
    10261 gtcttcttct ttgtttgtag aaaagatata cgtaaacttc ataatgactt ccataattgc
    10321 cattcctgca agttgttcgc tatttaatcg acccgaatca atctcttttt gtgtcggaat
    10381 gcgtattcct tgtgtcccta acacgttgat ttgtgaatca aaacgtatcg gcgtactgtg
    10441 ctcattccca atgagaatct ttcctaaacc aacgctatgc cctaaaatca atttagctag
    10501 ttctttgtca ctttttcgaa tttcttcgat aacttgactt aacgaatgct tacctggcat
    10561 ggtgtaaact acatcgatgg cgtcttggat caccgttttc ttgtggctcg ctgtttctcg
    10621 attggtatct acttctccta acattcctaa cacattttta gcggtttgaa ttgcctcttc
    10681 ttttggtaaa aacaatagag gatcaagcat ccctcgacag ctttcatctg aagataacga
    10741 aataaaatta attcgttcat acatagccac aaactctggt atgtgtccga atttttcaag
    10801 tgcctttaaa aagaactttt gggtttcatt tttcggatca atataaagaa ttttttgacc
    10861 taaaaacatc agccataaaa aaatatattt tacaaggacc gattttcctt tacctggtgg
    10921 accaataatc aacgtatttc cattggtatg ttccgcgcct tcaatacttt tctttgttaa
    10981 attgggataa taccatacta gtttactaga tagcgctaac gcttctttcg cagatttaaa
    11041 cttttttgca ataacaatcc gtcctaaagg aaatcctttc ttatttccta cttctttctt
    11101 taagtccaag ccataatctg ccacaaaacc tgtcgtagcg acctgctcgt aactacgaaa
    11161 acgttgcgta cttccaatta agcattgatt aaacaacgtt agctgatccg ctaccgatcg
    11221 ataaaatttg aaatctgtag actcaatcgc aaattcaata tctttgactc gttcttccaa
    11281 ttcttctttt gaagaagcag atacaaccag cgtgacggta gaataacaca atcgtttttc
    11341 tttgcgcttc agcttatctt ttacatccat taaccgttct gtaccaaaat ttaatacatc
    11401 gtctgaatct aaaatgccgt caacggtctc atattctttt tcttgttcat aaatccgtcc
    11461 agccatttta ttaatttttc gttcatcaga ttttgaatgc tcaaatcgaa gtcgaatatg
    11521 cgtatcaatt ggaaaagata agctatcctg aagattttga ataaagctac tcccatacat
    11581 ggctaaagga gcgtccgttt gacttaaaaa cgtcgaataa tgcgtttgat tcaactgttc
    11641 aatcgcaatg tatcctgctt cattcgaaat gacgccctcg caaatttcca ttggcgtaag
    11701 ctgtctttca ggcaattttt cttctgatcg atggaaaaaa taataataaa tttgtccaat
    11761 ctcttgggca gataagcgct ctatcggctt ataattggac aattccgtat agagctcctt
    11821 ttctaatgtt tcatacgttg gtaacacact acttatagga actcgttgtc ccgtaatacg
    11881 atagataaac tttttaaaaa catgcttaag caattgaaaa tattcgatcg gatcagacac
    11941 tttcatttct gtagtgaaac gcacaaacag atacgttcga tactcgttta attgcacttc
    12001 gtcttttaaa atctttcctg cctgttcaaa gtacactttc ccaagatctg cgcattctcc
    12061 ctttaccaaa tctcgaatgg tttcttcaat gtgcgtatct aggttaaatt gacgtggaat
    12121 attgataaaa tgatagtcat attcatcatg tccaagtatg ccttcgccat cttgaagata
    12181 gttcttgaaa aaatcaatgt catttaaagg aaatacttgg gaagaaagtt gatagcctac
    12241 ccacacatca tcatttttgg tcaaaacaaa atttcggata atggcgtcta tcggaaattc
    12301 taatcgagga agcttcgttt ttttcttttt gaactgccaa atcccgataa taaagacgac
    12361 caaaagaaac aaaaacgcca tttactcgac tcactccttc catttgtgta gggaatacgt
    12421 cactttttca tggaaaaacg gcacatacat ttcatgttca aagcgttgtt tccgtttctg
    12481 ataataggac gttcgcccaa ttaaatactc gaaaaagtgt ttattgtccc atttcattga
    12541 aaacaatgtc caaacgagaa caccaatccc aaaaatcata atcaaccagg actttcgaaa
    12601 cagtgtctgc aagaaactaa tttgattcac atacgcaatc aaaaatagga tcaaaagaat
    12661 gaagagaaat accccaccta tgactaattg ttccatgcga atgccattag aaatccaggg
    12721 aattttttgt cctttaatcc cgtaaatttt gtgagggtgc ttaaactcct ttgtatagtt
    12781 gtattccatg ttcactctac ctccttttac aaaaaaagaa gcgaccagaa gttctaatct
    12841 tctgatcgct tttgcagttt gtctttttct tatgataatt gtatccaggg cacaatcatt
    12901 tgcgttaccc ccacgttctc ttcactatcc ttaataaaga taatgttctt tagccattca
    12961 ccaatatcaa cggcattcga taacaataag acgagcatta tcaaaataaa gacgccaata
    13021 atggcaaacg ctgcccgcgc aaagccttgg gtcgcataag caccaaccat tagaacaatc
    13081 gcaacgattg ccaatagcca aatgacttgt ccagatacat catcaaataa cgatttaaga
    13141 tcaaaaagtt tcatttccat tctcctctaa gttcatttta ttcatttaac gtagtaacaa
    13201 aataactatc gccacttgtt tttacttcca ttgaaaagtt tgattggaac tcgtttcctt
    13261 tctcaaaata gaaacgatac ttcccttgca aaaagtagtt tccttcttta ttttttactg
    13321 catgatccag ttgtacattg atcaattcgc cgccttctaa ccctttttcg ttagaaatca
    13381 atgccaattt ttcatcattt ttgacatata actcaaaaaa ccgtttcaca aatgcttcca
    13441 gcttgatttc ttcgctttca gtgaccgctt gcccagaaga ataaaatcga ttttcctgat
    13501 agttcacaga attggtattc ttactctcag aaagattcaa aaattgaacg ccatcaagta
    13561 cttttaaaac attgttttga taactgactg gaaaagaaag ttgactgctt ttccaatgtt
    13621 ctccttctgt aaaagtaacc tcataaaata ggcgatacat tgcttgtgtc aaatcgattt
    13681 cttctagttt aataaagcga acatctttcg ctactcgatt ggtagccgtc gttacaaagc
    13741 ctagatcaga cgcttccaat ccttgtgcta aataatgatt gacggaagcc tcccattcgt
    13801 ttcgtttttc tggcgaataa gtaaataacc tttcaataaa atgttgtccc tcgtacttca
    13861 attgttccgt agaagacaag gtttggttga cttcagttaa caatgtttct ggattgactt
    13921 gttttttcac tgctaaacgc gttaatgtat ccactcgtcc aaagtttagg aggagcacga
    13981 cacccattaa cagaaacaaa caaaaaaaag cgcctgaaaa tacactcatg gcactttttt
    14041 gcgaaaattg gatcattcga tctgacggtt ctgttttctt tttttggaac aaaaaacgtt
    14101 tcttttgttt cttttccttc tgttgtttcg gtggcttagt ttggaacgct tgtggattaa
    14161 attttgcatt actcatgaga aatgccatac cgttctaaca ataatttcgg tattttgtcc
    14221 acaatcacta cttcgtggac aaccacactt gccccctc
//
LOCUS       DASJPC010000065        13908 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJPC010000065 DASJPC010000000
VERSION     DASJPC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.065"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..452)
                     /locus_tag="U4A13_001964"
     CDS             complement(<1..452)
                     /locus_tag="U4A13_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1780242.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGR"
     gene            complement(1324..2955)
                     /locus_tag="U4A13_001965"
     CDS             complement(1324..2955)
                     /locus_tag="U4A13_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN1780243.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2969..4642)
                     /locus_tag="U4A13_001966"
     CDS             complement(2969..4642)
                     /locus_tag="U4A13_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN1780244.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4655..4972)
                     /locus_tag="U4A13_001967"
     CDS             complement(4655..4972)
                     /locus_tag="U4A13_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN1780245.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4997..6397)
                     /gene="lacG"
                     /locus_tag="U4A13_001968"
     CDS             complement(4997..6397)
                     /gene="lacG"
                     /locus_tag="U4A13_001968"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN1780246.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6634..7419
                     /locus_tag="U4A13_001969"
     CDS             6634..7419
                     /locus_tag="U4A13_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN1780247.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            7422..8114
                     /locus_tag="U4A13_001970"
     CDS             7422..8114
                     /locus_tag="U4A13_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1780248.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(8388..8759)
                     /locus_tag="U4A13_001971"
     CDS             complement(8388..8759)
                     /locus_tag="U4A13_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1780249.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(9159..9389)
                     /locus_tag="U4A13_001972"
     CDS             complement(9159..9389)
                     /locus_tag="U4A13_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780250.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(9483..9821)
                     /locus_tag="U4A13_001973"
     CDS             complement(9483..9821)
                     /locus_tag="U4A13_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780251.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(10749..10865)
                     /locus_tag="U4A13_001974"
     CDS             complement(10749..10865)
                     /locus_tag="U4A13_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1780252.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(11341..12219)
                     /locus_tag="U4A13_001975"
     CDS             complement(11341..12219)
                     /locus_tag="U4A13_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEN1780253.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            complement(12542..13567)
                     /locus_tag="U4A13_001976"
     CDS             complement(12542..13567)
                     /locus_tag="U4A13_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1780254.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT         4392 a         2873 c         2292 g         4351 t
ORIGIN      
        1 cgcccattaa acgccgttac ctcttctgta aatgattttg ttatattgga catggtttgg
       61 ggcgtgtagt aatgcccata cattttctca atcaagtctg cgatttccga catggtaata
      121 ccttttcgga agaggtgaat gacggtctcc tctaacgtgt cattcgtccg tctataagca
      181 ggaacagtct gttgcttgaa ctcaccattg cggtcgcgcg gaatctggag atgaagttcc
      241 ccatactcgg tcttgaccgt acggtcatag gagccgttac gagaattacc cgtgttaaaa
      301 ccaatgcgat catacttttc gtaatctagg aaagccgtta actccgtttt gagaagcgta
      361 ttgactgctt tctccaaatg gacacggaat aattcattca aatcgccttt gttcgctaga
      421 gtctttagaa tttctgtagt aaaatcgttc atagggaagt cctctcttct gtgaatgtgt
      481 tgtggtaact ctattctaca gaagggactt ccttttttct atttacacaa aatattttac
      541 actctccatt ataacaattg ggttaaaaag aaaatagata gtattctacc tcatatttat
      601 ttgaaagtaa tatataaaag acgttcaaat tcgaacgcct ctcgttaacc aaatattgga
      661 ttcagaatct agttttcata tataaacaga gtatgattag gtgcaaaaat tgcactttta
      721 ttgcactttt atttttattt atagtgtttt actgttctgt atattgtttt aactcccttg
      781 atagtatcgc attctgtttt atacacttta cgttgaaaac agagtgggaa taaagagcca
      841 acataatatg gagggggaat ccctataaat caacattctt caatagctaa aatgcttgat
      901 tttcattaaa aataagatgg ttccctacca aaagagacta taatataagt taatttgttt
      961 cattcttatc ataaaaagaa gttctcgaaa aacgaactgt tttccgagaa cttttttcta
     1021 tccatctaag tgctcaaatc atattcacga cggaattcta aggttaccct ttcttaaaca
     1081 aactttatgt tatctgcaca actttttgca attttttcat tgcttcttac gaaatcgttg
     1141 aactctcttt agcataatac aaagtgtaga tgaagtctgg tgctttactc gttttggtaa
     1201 aaatgactgg aaatcgtttc tggttatttt tggcaaaggc gaggtgcagg ttcgattcct
     1261 gccttctacg ctaacgatgt ggctgaaggg aacctccgat cttgaatcga aacgatccgc
     1321 agtctattgt ttcaccaatg aaacaaaatc aacgttcgtc caagcatttc ctgaagcctt
     1381 gctataaacc ccaatttcac aaacacctgt tgtaattgga atgtctttga ttgtaatttt
     1441 tgtccattca cttgttttaa caaccgcagt atttagttcc gctccaccat aatttttgat
     1501 atataactga ttagtatcaa actctccccc agacattaca taggcactca atgtgtaagt
     1561 tcctttgctt atgccactaa cattttgata agctgatgcc tcgtatgctt gatcctgcca
     1621 aaaagttagt ttgtagtccc caaaaaaagt gcccgcttcc gttttgatcg tacccttagc
     1681 ggttccttgt tgaaaccagg tttgccaatt ggaaggactg gtcgttggtc catcgctttc
     1741 gaaacttgca tttttaacta gatttatttg cgcccctgct tgtttaaacg cacgcaagct
     1801 tggcaaggcg tttccagcaa aatcaaacat cgcttggttg tcccattcgt ttcccatagt
     1861 ggattgaaca ctgagataat tcatacctgc ttgtgttgcc caactcacat ttccgttgta
     1921 ccaaagaggt tcccaataga agaagcccgc cccgcgatta ttcggtactg cctgaatccg
     1981 atcggcaaga tccagcaaat actctgcttg tcctgtcggt gttggttcat atccccccgc
     2041 agctgcttcg gcttcaccaa aagcatttgg catagtatcg gcattcgctg ttgtatgccc
     2101 ataagctgtt tcaactacga tcacttcttt accataccgt tggctgatcg cattcatatt
     2161 ggtttgaagt tcattcattg aaccgtgcca atacggataa tacgacactc caattaaatc
     2221 aaatgacaca cctcgactcg tgatttcatc aaaccaccaa gtaaatgctc ctgtatcgcc
     2281 accatcagct aagtggagca ttactggagt tgttttaggt tgagtgtctc gaacagcttg
     2341 gataccagac ttcaaaaaag cagccaatcg atcaaattct cccccgcctt ctccccaaga
     2401 cttgccataa ggccagagca ttccgctatt cagttcattg ccgatctgaa ccatatcagg
     2461 ataaacgtcc agctgcttca tttccgacag aacgttcttt gtatagtcgt acaatgccgt
     2521 attcatttct tcaaaggtca gaccttgcca tgctttagga agattctgtt tcccaggatc
     2581 aacccaaaaa tcactgtagt gaaaatccag cagcacctta agtcccagat tttttgctcg
     2641 ttttgcaagg tcaatcgtcg tttccagatc atttgtccct gctccataac tttctccatt
     2701 actattataa ggatcctgcc acaaacgcag tcgaacatag ttgacaccat tattttttaa
     2761 aatctctaag gcatcttttg aaatatcgtc ttcataatat tttgcaccag atttttccat
     2821 atctgacagg atcgaaatat ctgctccgcg aataaattcg ctggcagatg cttcaacagt
     2881 ttttgaaaca gcccccaaag cccctaacac agctgcaaat aaaacagctt tacgcaacca
     2941 aattcccatt ttcattatgg ctcctccttt attgaaattg gttcagaata aactgaacag
     3001 ctttttcagg atctctcgtt aatccgatat attctgctcc ttttgtcgca accagcttga
     3061 ttcctaagcg atcggtgtct tctttgatat catcatagta agaattgact tgcggtgcta
     3121 agaccaccat atcaaaatcc tgcagcattt cgtaatgaga accataagca cccgccattg
     3181 cactgatcgg tgctttcgat tcagatgcac ctttttttaa agcattagct aacatcgcac
     3241 ttgtccctgc gccagcacaa agtaccaaca cattgatttc tttatttgca tcgctgataa
     3301 cttgactcgc tgccacaggt acttcctcac gtacatcggc agaagcacct tcctcggttg
     3361 cgatcacctc tgctttttgt gcttctttct ctactaagac agcatcgtat gctttgcaaa
     3421 atggtagata gatcaggaag tcaacgacga ttagaaccgc tgctagaaca aaagctaaga
     3481 tacccatgcc cgttcctaga accaaaccga tcggtgctgg agttgcccaa ggcaaaacgt
     3541 acataaagct gttcatgccc aatacatcga caaaaaactt gaacaggcaa acgtttacaa
     3601 caggtgttaa caaaaatgga ataaaaaaat aagggttcaa aatcatgggt gccgcaaata
     3661 gcaatggttc gttgacagcg aaacttactg gaataaagga agctttccca actgccttca
     3721 actgcttaga cttagcaaag agtaggaaga taaaaggaac aaccaatgtt gccccagttc
     3781 ctcccatagt tcctacaaaa ttccctaaac ccaccgtcag tactttagaa gcttgttcac
     3841 cggaattgaa caattgcaga ttggtttcca cattggcata gataattgcc gcaattgccg
     3901 gctccacgat cgacggtcca tgaacaccga caaaccaaaa cagcgccatc gctccccaga
     3961 taatagcgat tcctaagtac ccgtcagctg ctgtaaatag cggctgcagc accgtaatga
     4021 ttgcttctgc aaatgagatt tggaagattt gtcgaatgcc taaatcaatt aaaacggccg
     4081 caaatactga aaaagagaaa ggaaaaatat ctcggaaggt ttgcgaaatc gttccaggaa
     4141 cttctttagg cattttaatg gtaatatctt tcagcacaca aaacttatat aaattaactg
     4201 ttaaaaatgc agcgacaaag gaagccagta gtccctttgt ccccatatat tccgttgaaa
     4261 atccaccttc aatcggagag acgcttaaca acaggaaact taccacagag gccagcatca
     4321 cagaggtggt gttgattact ttgtttttcg gtaagcgacg attcatcgaa tcagccaagc
     4381 agcgagcagt ggttgctgaa acgagtaacc ctacaacacc catcgaataa ttatagactt
     4441 tccatagcca tgtggagaca gattcaggcc attggtaacc aaaaatctca ggaaccgcag
     4501 caatcaaaat gaaaatgctt gaaaataaaa tagctggcat cgctgttaaa aatccgtcac
     4561 gaacggctcc taaataacta ttacgtgaga ttttttcaaa aaatggtttc cctttttcga
     4621 tttgtttaat gattccatcc atagcgttct tctcctatcc ttgattctgt ctgtacagta
     4681 caataaagtc ttgaatcaga tctctcagca ataaagtggt cattaaatga tcctgcccat
     4741 ggataaaaat gaatcccatc tccatctctt tcccagatgc ttcttccgct aaaatttttg
     4801 tttgcgaatt atgtgcaagg gttaaatttt cgtcggcttc tttcaacgcc gaatcaacat
     4861 gttcaaagtt tccttgtctt acttctttca ataatttcaa taatgtactg cgagcgtcgc
     4921 ctgaatatgc aacgatctca aaaccaagca tttgtaattc ctctttattc atcttttctt
     4981 cctccattat cacttattaa ataatcgttt tggtttcact gaccaacttg taccagtaag
     5041 ctgattcttt tgggtaacgc ttctgggttt caaaatcaac ataaaacaag ccataccttt
     5101 tgttataccc atttgtccat gagaaaagat ccataagcga ccacaaaaaa taccccttca
     5161 catttacccc ggctgttatc gccttgctta aggattccaa atagactctc aaatagtcga
     5221 ttctcggctg atccataata atgccatctt caaactgatc tttatacccc atcccgttct
     5281 cagtgatata gattttgttg taatgtgggt aatcgctttt aattcgcagc aacaagtcat
     5341 atagaccttc tggataaatc agccagtccc aatcagttcg aggaattcct tctttataaa
     5401 tacgttctcc gattcccttc actttataaa cagaggtccc tttttcaccc gtaccgttat
     5461 gatggattgc attttctcca tcgtaagctt tgacaaaatg gcattggtag tgattgatcc
     5521 ctaagtagtc attacgcgtg gatgcttttt tcaattcaac gaaatcttct tcaggaaaat
     5581 gatagctggc ctgatttgct tcacatatct catcaagagc agtcaaggtt tcagttgaat
     5641 aatagcctaa ataggtagca tctaataaga aacggatcga caatgcatcg tctaaaaagg
     5701 cagcatgctt atcttccggc gcgtctgttg ccgcatattt cgtctctaat gagtgaacta
     5761 caccaatttc acccggaagt tcgttttctt tgaaatagtt cactacacgg gcatgagcaa
     5821 ccatcatatt atgaagacag gcgacgatct ttgtaaaatc atatttgatt cctggtggga
     5881 aaacacctaa caaatattga ttggttgcaa ctggatagat ttcattaaag gtactccaca
     5941 ccttgacttc tttgaattca tgaaaacaaa aaatggcata ggaaacaaat gcttctattg
     6001 tttctcgatt caaaaaatct ccatggtcaa acaaacgttt aggggtatca aaatgatgca
     6061 acgtcacaaa cggcgttacg tttcttttct tacattcttc gaacacacga tgataaaaag
     6121 cgattccttc tggatttggt ttcccagctc catcaggaaa aatgcggctc caagcaatgg
     6181 ataatcttag accatttaca ccaaaacgtt cacacaattc aatgtccttt gcatattgat
     6241 gataaaaatc acttgccgga tcgggactga atcgcccttg ttcctctaaa aagtcatccc
     6301 aagcaacagc gcctttgcca ccttccttag tggctccttc aacctgatag gctgctgtgg
     6361 ctccgccaaa aataaaatct tctggtaatt taagcatgta catctctcct caaatctata
     6421 atcagttttc gctctttcta actgattgca ttgtaaacgc atccaaaaca tttgtcaaca
     6481 aaaacaaaca caaataatca taaaaaccag aaaacagcaa taaaatctat tgtaacaaac
     6541 ataaaaacca ccctaaatgt ttacttttgt tcgaattaga ttgactgttg tccgtttata
     6601 tgatagatta tccttaaatt agaggtgaag acaatgttga aaaaagaaag attgcttact
     6661 atcgtagaaa tggtcaataa aaaaggaatc cttaccgtta atgaaatcat caatcaatta
     6721 gatgtttctg atatgaccgt tcgcagagat ttagatgaat tagaaaaatc gggcaagctg
     6781 ctgcgtgtcc atggaggggc tcaaagtctt tcctacactt tagatcaaga actttctcac
     6841 actgaaaaag ccactctgca gattcaagaa aaaaaagaaa ttgctgccac agcagcctcc
     6901 ttgattcacg agggagaaac gattttctta ggaccaggta caacaatcga actattggcg
     6961 acgcatttac tgaacaagaa aattcggatc attacaaata attatccagt ttttgatatt
     7021 cttgctcaag cagatggacc agaaattcta ctgattggcg gtgatttccg gaaaaatacc
     7081 ggcgcatttg tcggaccgat caccaacgac aatttaagaa gactaaagtt tacaaaagca
     7141 tttatcagtt caaatggcgt tcacaacgaa gcgatctcta cttacagtac cgaagaagga
     7201 gaagcacagc agatcgctct aaataattcc cgtaagaaat atctactcgt tgataataaa
     7261 aaattcaatc gagatgattt ttatgtgttt tataatttgc atgatttcga tctgctcatt
     7321 acggatcgcc agatctcgcg ggaggttcaa gagcactatg cacaatatgt cgatctgcaa
     7381 attgctgatt caaccgaaaa taccactata ggagcgtaac tatgaaaggg attatatttg
     7441 atttcaacgg aacaatgttt ttggactctc atctgcatga agctgcttgg ctgcatatga
     7501 tacatgatca tacaaaagat ggtttatcag acgaggatat tttaaaaaat attcatggtc
     7561 ggacgaatac ggagattttg aaacatttca tttcagagca tttgaccgcc tctgagatcg
     7621 ccgtattgtc agaagaaaaa gaaagctatt atcgtatgct ttgcttgcaa aatgaaaacg
     7681 agcttcagtt gacaaaaggg cttgaagcga ctcttgacca gctgactgac caacagattc
     7741 cattaacgat tgccacggct actgtcaaag agaatgttgc tttttatttt gatattttcg
     7801 atttagcccg ctggtttgac tgggacaaga ttgtctatga cgacggctct ttccctggga
     7861 aaccgcaacc ggatattttt cttaaagcgg cagacaaact gagcttagct ccggctgatt
     7921 gtcttgttat cgaagatgct tactcaggat tacttgctgc caaacgtgcg gggatcggaa
     7981 caattattgc aattgatcca ttcggtaaaa acaaaacagt atttgaacag gcacagctaa
     8041 ataaagatgg aaccatcaaa gattttactg gtttttggac agagcacctg tctgccacat
     8101 ccgttaattc ttaaacgatt caactaacta ccaaagaaaa gcaactgaaa tgcacacctc
     8161 cttttataaa aaactgccag tgaaacgaca tgaaagatac actgctatat ccttagtgaa
     8221 atatagcagt gtatctttga ctttttcatt cttttaacca tatgacgcgt tcatctctat
     8281 ctataattaa cattaaaaat agtgttttca cattatttct caaaccaatc ttaaaaatca
     8341 tcaggagtac ttttttgata tacttttatt aatgctgggc gtaatggtta aaagatttcc
     8401 actagttcct ctccaccaag ctgagttgaa aaatgcttat ctgtaaacaa catagacaaa
     8461 gaggaaataa acgtttccgt atctttctct gtcccaagga tattaattaa ttttcctact
     8521 aggtaagtat cattaaactt aagatctgcc ttcacatcaa aagttttgat aaaagctttt
     8581 atcatgcgta caattttgtc tttatcgcca taattgattt caccactcat atcaatatag
     8641 tcactaatcc ctgaagaaat aatggcaact gcttcttctt tcgccttatc agataattca
     8701 tttaatgact gggattgatt gatgacttct tgagttttat ctgttacgta atctctcatt
     8761 atcctatcgg catactccgc aaagtaatct tgaaaaaatt cttctacagg gtaaccaact
     8821 tcgttttccc aaccaggaga aatttcatta tcagtactta ttaaatctat taaaaaccaa
     8881 cccgttctct gagtgttgat tctataatat ttttaatttc ctatataaat caaaaatatt
     8941 atagaatcaa taagctaatt aagaaacaaa ctttagaaac tctagaagaa cttctgaatt
     9001 tatatagaag tgtgtagcta cttactaaat atacaaaagt taaaataaaa tactctggat
     9061 actctttata ctgcattaaa gttagtaata ttaacagcaa agatatcgaa aaaacaataa
     9121 cataatatgt gtcaaaattt ttcataataa gaactccctt actgttgcat actaacataa
     9181 tccaaaaact cttctacttc agctaaagac attccacttt ctttagtcca aaaaacagtt
     9241 ttttctatcg atccttctct gtaaactgta aatgtcggag tcgagttaat tgagtagcga
     9301 ctcttgaatt ctttccactc attttcatct tctctaactt ttgctacatt cacgtaataa
     9361 atattttctt ttcccttttc taccagcata tcgaatagct tcctgctttt gatgatcgat
     9421 ttcttgtgct acatctagta aattttcatc aatttctata tcataaagta tttcactagt
     9481 gtctacatcc acaatatata ctttttatca gtagcaattt ttgagtaatc cgttaaaatg
     9541 atctcagtct cgtcactttc atcagtataa atatacgtta acaatgaact caccttttca
     9601 atcggtgtta gctctaagcc tactgtattt tttactattg agtcattatt taccaccata
     9661 acttcgtact gtatatacca tcctatttgg tttccatcaa tatcagtaat tggctcacta
     9721 gctaatggag tctccggaac aatattttcc tgatatttct taaactcttc tgtagcacga
     9781 aaaaggttat catgttcctc acttgaaact ggttgagtca tagaaaatgt tacttggcta
     9841 aacagaggga aaataaacac tacaatcaac aaagtataaa aaaaagccct tctttttcat
     9901 tttttatctt ccaatcacaa attgaaaaac ttattcctgc aactttgagt ctagtatcac
     9961 aatcgactga ctacaactat tcatttacat tctactttaa gttcctcctt aaccattaat
    10021 tttattaaag atacttgtaa tagtgattct cacaaaattt catgaaaaat actttattac
    10081 tgtatctaaa attccaatac acaacttatc gactattatc tgaaaaccaa aacttattat
    10141 tccttgatgt tattctagca atagtaactg cttttttatt tttttgcata ttttttcggc
    10201 gttacttttt aaagttaact ttttcgacaa atagaaagag atacgaatca ctgatttttt
    10261 tgaagaaaag aaatggagtg agctgaacgt tagagatatt gggagctttc gtaactgctt
    10321 attaaacgat aatggttatt tgcaaccgta ttgctcgctt ctacatgaaa tatttcatga
    10381 aatatttcgc aaaacctttg attatgtagt tacgtttcca ttcttgacta aaaatgtttc
    10441 aaaaacgaac aaaggtgtta aaaggtaaag tgatcaatac tactggtcaa aataagaggt
    10501 tgaaagggaa ttgaaaaata actacagata atacttttat aaacgtacgt gctttgtggt
    10561 tctgtagtta actatataac tggaattagg gaatggtatc aatttgaaaa gaaagattct
    10621 accaatttat catatgttaa aaatggaaaa tcaacagcag ttctttcgga aaccttacac
    10681 aaaaaccgat tattgaaaat agccgaaaaa cgtttctcgg ctattttttt aattaagttt
    10741 ccttcttctt accttttccc actattccgt ttcacatctt tgatgatctt cagcgcacgt
    10801 tttcgtgttt tgatatttgg gcttttcaag cggcggtatg cacttgagat atctgttttt
    10861 tccatatgga cgacccactt tcttctttga ttgtagtcat gctaaaaaac acaaatcgaa
    10921 acaattacga tttgtgtttt aaatggtatc aaaagttaaa atgaaagtca acttgttaaa
    10981 acagtcgatc agactagctg attgactcct atctctggaa ataaattttc attattacgt
    11041 tttagctacc taaaaaaggc cctcttgtcc gttaacttta atatctattg ttatagctag
    11101 tttacctatt gaacgtactg tgtgttcgat agaacactaa aaaagaggct gggacagaag
    11161 cgtttaactc caagaaataa gaagaaattc acgaaaattg cttttcaaat ttttgtgaat
    11221 ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt ttagagcgtg
    11281 agacaaaagt gttctttact tttgtctcac gctcttttaa actatttcga agtactaaac
    11341 ttatgaagta agaataccta agccagagcc aatgtgctcg cctgcaagca attgctcttt
    11401 tgcttgtcgc tgtaaactcg tactttctgg ataatcatgt atttgggcat ctactcgtcc
    11461 aaagatccaa gggactccac tatcgtcctg ttcatcaact gataataatc gataatcggc
    11521 tgcaaaaaga cagatttttg actctttttt caaccattta gtaagattgg gtgatagtaa
    11581 ccaagtatct gtgtacatca cttggtaatg gtaatcagga aaataaagtt caaaaaaacg
    11641 aatggcactt tcgtaactat ctcttatctt attctgagat aaatctgcat cggaaggaat
    11701 atgaagagaa aggcactgct ttccttctaa tcttagatcc gtgtaccctt tcggaaaata
    11761 tgtcatttcg tactccaatt caccaatccg aaagatcaag ccactagtgt aatgccaaat
    11821 ccaaaaacca cggtcaaagc gatagcttcc tgttacttgg aaatgttcat gaataaatct
    11881 agtaaaagca ttcattgttg ccaaatagat agttcttgga atcttcaatg gttgataaat
    11941 ttttttccaa ctttgagaag ctgcatatag ataaatagct aatgctgcaa aaggttcctc
    12001 ttcatagact ttgtctattt tctttgcagt cgcctttgct ttattaggat ccattagttc
    12061 atgactatat tgctcaaaca cctcatcaag gatgtttttt gcttgtacaa tcatttgctc
    12121 tataacgact ttgggaagag ttaaagtttc tgccagaatt tggatttcat tgcttatttt
    12181 cttcatcaaa tttccccttt tcatatcttt gaacctcatt gtttaaatga ctgattatcc
    12241 ggacaccata tggtataaac atacttttat tgtacactaa ctataaaact taaactttct
    12301 atgaagaaag aatatctgta agactttcaa ggaaagcatt gaccatctca ggaaagttta
    12361 ttcagttacc atcataaaca aaattttttg atagaataaa tataaaattt ttttgaaagt
    12421 tcctttccac atttttgtgt gaatttgact taatgctgag aatctggctt ttgaacacct
    12481 tgaattagag gttgtgacaa aaatcttgtc ataacctctt ttggcacata tatttgtcct
    12541 tctattctaa tttttgacga acaaccgtta aataatgttg caaaccaacc gtcattaccc
    12601 acgcaacacc taaaccgatg aaacttccta tgacgatccc taaaaaacga tattcgatca
    12661 aagaaatcaa gtatgctcca cgaataagtg tagatagcat caacgccatc ggattaagaa
    12721 aaatattagc aactgcataa tttctagcaa ctagaaattg tgcgattcca tatagaaaag
    12781 tgatcagaaa aatggtctgc aactgcgtaa acggcacaaa tgacaagaat gccgacacac
    12841 ataacccacc catcgatcca atcagatatt ggacttgtcg atgcttcatc gcatccaagt
    12901 tttctgctag caaaatcgat gcacatgaca aggtcaacca ataaggatta tgcagattca
    12961 agccgtgact gacataaacg gaaagaaata aggcagcgct gtagaaaaca gaatcaatga
    13021 tcactaaagg ctcttcgtga aatctttctt ttaaagttgc tttttcaatt gtctgctcag
    13081 gccgtgaatc cagtaatttt gtaatcactg ccgcaatcag ggcaaaaacg acacccataa
    13141 aaaaataaag tgagacgatc ggtaattgag cgattggcag ctgcatgctc gttcccatgg
    13201 cagaaagcat cgcaaaaaac aagccacctg gtttagaaat atggaataat cgcaacacga
    13261 atctgctgga aaaagcaacc aaagctacgg caaatggttc aagccataaa acataagtcg
    13321 ataagatccc caaaacgaaa gcaaccaaca gtccgcaacc gatcagaaac atacgtttca
    13381 gcaaatattt catcggtatg ttttgataat agaaaaaagt aaagatccct aatgatgaaa
    13441 aacttgaaat caaaaaatca tgatttatgt aacctaacca taatacaata aagataagta
    13501 gcatcagatt catgatacga aaaggattat cttgtatttt attgaaacga aaataagtct
    13561 tgaacaatct atccctcctt cctagtaatt ggaaaaaaag ctgaaccaag atgaaatgcc
    13621 aatcccgttt gtcagcatta cctagtataa caactatttc ttgatttttc ataaacttag
    13681 tatcaaaaag taaacaactt ggagaaatta gaacaaaaaa cgaacattcc gagtcgtttt
    13741 gacagaaaaa agatagcaac ttttccaagc aactcgctat cttagctttg ttaaagaaac
    13801 cggattccca acaaaaaaaa gcttctaatt ttcgtacgat aactacgttg gtccttaaac
    13861 ttcatacttt tttatgattt ttcagcggtt ttcgatggta tacttttt
//
LOCUS       DASJPC010000066        13732 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJPC010000066 DASJPC010000000
VERSION     DASJPC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.066"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(143..1345)
                     /locus_tag="U4A13_001977"
     CDS             complement(143..1345)
                     /locus_tag="U4A13_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1780255.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="U4A13_001978"
     CDS             complement(1348..1542)
                     /locus_tag="U4A13_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1780256.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="U4A13_001979"
     CDS             complement(1589..2437)
                     /locus_tag="U4A13_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEN1780257.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="U4A13_001980"
     CDS             complement(2642..2992)
                     /locus_tag="U4A13_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780258.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="U4A13_001981"
     CDS             3587..4033
                     /locus_tag="U4A13_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN1780259.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="U4A13_001982"
     CDS             4657..5649
                     /locus_tag="U4A13_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1780260.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="U4A13_001983"
     CDS             5665..6549
                     /locus_tag="U4A13_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1780261.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="U4A13_001984"
     CDS             6598..8205
                     /locus_tag="U4A13_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1780262.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="U4A13_001985"
     CDS             8206..9690
                     /locus_tag="U4A13_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN1780263.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="U4A13_001986"
     CDS             9867..10859
                     /locus_tag="U4A13_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1780264.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="U4A13_001987"
     CDS             10911..11780
                     /locus_tag="U4A13_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1780265.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13693)
                     /gene="guaA"
                     /locus_tag="U4A13_001988"
     CDS             complement(12200..13693)
                     /gene="guaA"
                     /locus_tag="U4A13_001988"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN1780266.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4214 a         2322 c         2832 g         4364 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tt
//
LOCUS       DASJPC010000067        13730 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJPC010000067 DASJPC010000000
VERSION     DASJPC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.067"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            39..1451
                     /locus_tag="U4A13_001989"
     CDS             39..1451
                     /locus_tag="U4A13_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1780267.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESST
                     ISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQS
                     QPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQL
                     AQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLT
                     KGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEV
                     KGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFH
                     YSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEIC
                     KDSSLSQSMLVDPKTLLFGD"
     gene            1800..2432
                     /locus_tag="U4A13_001990"
     CDS             1800..2432
                     /locus_tag="U4A13_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN1780268.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3020..4459
                     /locus_tag="U4A13_001991"
     CDS             3020..4459
                     /locus_tag="U4A13_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1780269.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4754..4972
                     /locus_tag="U4A13_001992"
                     /pseudo
     CDS             4754..4972
                     /locus_tag="U4A13_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5221..5436
                     /locus_tag="U4A13_001993"
     CDS             5221..5436
                     /locus_tag="U4A13_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780270.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5653..5909)
                     /locus_tag="U4A13_001994"
                     /pseudo
     CDS             complement(5653..5909)
                     /locus_tag="U4A13_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6096..7007)
                     /locus_tag="U4A13_001995"
     CDS             complement(6096..7007)
                     /locus_tag="U4A13_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780271.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7026..8030)
                     /locus_tag="U4A13_001996"
     CDS             complement(7026..8030)
                     /locus_tag="U4A13_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN1780272.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8027..10150)
                     /locus_tag="U4A13_001997"
     CDS             complement(8027..10150)
                     /locus_tag="U4A13_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1780273.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10155..12602)
                     /locus_tag="U4A13_001998"
     CDS             complement(10155..12602)
                     /locus_tag="U4A13_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1780274.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12589..12978)
                     /locus_tag="U4A13_001999"
     CDS             complement(12589..12978)
                     /locus_tag="U4A13_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780275.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13038..13541)
                     /locus_tag="U4A13_002000"
     CDS             complement(13038..13541)
                     /locus_tag="U4A13_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1780276.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13554..13715)
                     /locus_tag="U4A13_002001"
     CDS             complement(13554..13715)
                     /locus_tag="U4A13_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780277.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4220 a         2710 c         2509 g         4291 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcatc aagtgaaagt agtacgactt
      301 caaatgccga gtcttcaaca acttcgtcaa gtgaaagtag tacgatttca agtaccgaat
      361 cttcaacaac ttcaagtagt acaagtgaaa taacttcttc gaaagtgaca aaaccaaacc
      421 atatagaatc ttcaaaaaag gaagcgccaa aaacggaagg acaaacaaaa actttttcga
      481 caccacagca aatagaatca acagaatctt tccaatcaca accaatacta tctggtaatg
      541 aagaaattca ttttgacaaa aatgaatctg tagaaaattt tattatgaaa attggtgagt
      601 ctgcgagaaa aattggtcag aaatatgatt tatatgcttc tgttatgatt gcacaagcga
      661 tcttagaatc tgccagtggt caaagccagt tagctcaggc accgaattac aatttatttg
      721 ggataaaagg aacttataat ggaaactttg tcattatggt aacaaacgaa gatttgggta
      781 atggaacgct ttatacgacc caatcaaaat ttagagtgta tgaaaactat gaggaaagct
      841 ttgaagatta tgccaaatta ttaactaagg gaattagtgg aaataaagat ttttatgcag
      901 gagcattaaa agctaatagt aaaacatatc gagaagcaac taaattcctt acaggacggt
      961 atgcaacaga tacacaatac tatttgaagc taaatgaatt aattaaaacc tacgatttaa
     1021 ccaactatga taaagaagtt aaaggtccac aaatgagtaa gactggatac caagtgccta
     1081 ttcataatta ttcaataacc agtttgtttg gaaatcgtgg aaatgaattt caccgaggac
     1141 ttgatatggc agctacacag ggtgagccga ttcatgcagt taaagctggt aaggttgtta
     1201 aagcagagtt tcactactct tggggaaatt acgtggtgat acaacacgaa gatggatcaa
     1261 ccgcgttata tgctcaccag caacaatacc tagtaaaaac tggagataat gttttgcaag
     1321 gacaaataat tggctatgta ggaagtactg ggaatagtac tggcccacac cttcatttgg
     1381 aaatttgcaa agatagtagt ctttctcaga gcatgttagt tgatccgaaa accttattat
     1441 tcggagacta aaaaaggtaa tcaatctgac aaagtaacaa gaaaatggtt ggattcttag
     1501 acctttgatc aggatattga gactggaatt gatatctcga ttacgatggg tttgacaaat
     1561 aaaacaagtc cattatcgaa tatcgagtgt attattgccg tctttatgct catattttga
     1621 gcaggtttca ctagatgaaa acaatttatc tatcttgagg atttcgtgta cgtatcgttc
     1681 agtcttgtat tgtaaattag tcataaaatc agactaagaa aaattggaaa tatttttttc
     1741 tgatttatta aaagttaatt gaaattatat ggtacttctt tcttgggagg gtaggtgcag
     1801 tgaaggaaag atatggtcac cttactgttg ttgaggagat tactcataat ggatataaaa
     1861 aatatcgttg cctatgtgac tgtgggaatg aaacgattgt atatcgatca aacttactat
     1921 caggtagaac caaaagttgt ggatgtggaa attataaaaa taggcataaa tataaaaatt
     1981 tgacaaataa aacttttggt aagttagttg caatcaagcc aactaaactg cgaagtgaag
     2041 gaactgttgt ttgggaatgt caatgtctgt gtgggagaaa aaagttagta tcggcaaagc
     2101 gacttctaaa aggtgatgta aaaagctgtg gatgtttgaa atcaaaaaag catcacattg
     2161 aaaataaaaa attcggatta cttactgcaa agtatccttt ggacaataac tgttcatgga
     2221 aaacaaaatg ggtttgtcat tgtgagtgtg gcagtatgtg tgagataagt tatagtaact
     2281 tattttttgg acatactcaa agttgtggat gtttaaaaag gcaagaaaca aggacatgtg
     2341 ttgaagggac agtaatagag tgtctaacgt caaaattacc gaaaaataat acaagtggtg
     2401 ttaagggagt ttatcgttct aaagagaaat agattgcgta tataacttta aaaaaaaaga
     2461 ggtactattt aggatcgtat gattctatta agagtgcagc tcaagctaga aaagatgctg
     2521 aaaataaatt gtttcaacct ttgattgaca aatatcgagg taatcattaa ttattgtgga
     2581 taataattaa cagtgatata taaacatcta taatatatta ttcatactat tgatatttag
     2641 tataatccta tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa
     2701 taggtgcaac accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg
     2761 tctcacgaga tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg
     2821 ctagaagaaa gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc
     2881 tccaacttac atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta
     2941 gagtcgcgag aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca
     3001 ttagaaagga aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg
     3061 ctggtggatt ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat
     3121 tgtcaatatt gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga
     3181 atgggtagtg tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat
     3241 tggcgatagc cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg
     3301 atcgctgttg tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca
     3361 agccattggt gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc
     3421 catacatgaa cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat
     3481 tgctggacca ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg
     3541 gatcaatgta ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga
     3601 tatcaccatg agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat
     3661 tatcagcttt tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc
     3721 ccttgtactg ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa
     3781 agcagcctct ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt
     3841 gattacagcc ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta
     3901 tctgcaaaat gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc
     3961 tttgatgatg gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga
     4021 gattttggta ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt
     4081 gtcagtggac tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa
     4141 tgccttgttc cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt
     4201 gggactggca ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc
     4261 gctttctacg acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa
     4321 ctatatcgca aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag
     4381 ctttgttttg tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg
     4441 tattcagaaa aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg
     4501 tgagtgggat ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca
     4561 agtattatct tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg
     4621 cgaaagccgc tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta
     4681 ctggttatga atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg
     4741 taaagatttt cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag
     4801 tcaaattggt tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg
     4861 gacttgtcct atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt
     4921 gaccaagggc tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg
     4981 gggatagctt ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca
     5041 agaattccgt ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt
     5101 tgatttaggt tccttttcta attataataa gatgaccact acagtaacct actgaaatat
     5161 ataaacagcc actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt
     5221 atgaacgctg gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc
     5281 aattcaatat caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt
     5341 gaaagatcag tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg
     5401 aaaaatggaa aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc
     5461 acttagctga atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat
     5521 acttgcaagc ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta
     5581 gttctaaggc ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct
     5641 tagaaagtct cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt
     5701 ttaggttgtg ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag
     5761 gtaatgtgat gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca
     5821 tcatcaatga catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa
     5881 actgaatgat tatttgtatc taattccatg gtatatcaac catttcctat atagactgat
     5941 tatgacatgg attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga
     6001 atggcattca tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta
     6061 cttataactt ctaataaaat tagattgagt cattattatt taacatcaac gattttccag
     6121 tttccatttt tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta
     6181 tcaaggtatt ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga
     6241 ttctctaact ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca
     6301 taatactcca attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa
     6361 tcggtgattt cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct
     6421 tcatattttg ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc
     6481 ctgttttcat cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt
     6541 ccctctgtaa tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca
     6601 acatctagaa tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata
     6661 tcttggatta aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt
     6721 tcaattgctt ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa
     6781 ctttcaacgc cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta
     6841 tgttgatcaa tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa
     6901 agccaaaggg caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc
     6961 ttttttgggc tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt
     7021 ttctcttatt tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga
     7081 ttcgtccacc ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc
     7141 acatgggtca cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt
     7201 ttggcatctt ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga
     7261 agtttcaggt caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt
     7321 tctggacttg agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa
     7381 atttcttgga ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa
     7441 cagtaaaaca tattgccata attataccgc cacccaccat tttccttaat cgcgattggg
     7501 ttggggtaat cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca
     7561 aaactatgct tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag
     7621 gcctgaataa tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca
     7681 aaatagagac agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg
     7741 gctgactcag aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc
     7801 gccaaaagaa ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt
     7861 gcacggtgtt tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta
     7921 ccatttccct cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa
     7981 gagatggtca gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac
     8041 ctgaccttga ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg
     8101 gcagagtgga cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt
     8161 tcccttttga aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg
     8221 ctctcacaat ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg
     8281 ctttatctat tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta
     8341 tcttgcgttt ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca
     8401 ctcgatccgc tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact
     8461 tttcgattcc tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct
     8521 ttacttgttt agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc
     8581 tcccagccaa attcattcgg gattgtttct tattatttga aactggattc tccttagtta
     8641 agtcagatag tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg
     8701 atggcttaaa tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac
     8761 caccaaaagc caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta
     8821 acatttgcgg ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt
     8881 tgaacatact cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca
     8941 gaaaagcaac catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc
     9001 taaatgcagt cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac
     9061 gaataatcgc cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa
     9121 ttggcagaaa catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta
     9181 gaagaaaaac aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta
     9241 aacggttcat ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct
     9301 tgaccgcttc ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt
     9361 tgacccggtc ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag
     9421 gttgttggac ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt
     9481 ggctttctga attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat
     9541 ctgagctaaa gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg
     9601 atagtagaaa aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt
     9661 ttaataatcc ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc
     9721 caaaataaaa tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat
     9781 tctttccaat actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca
     9841 cataacccgt ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat
     9901 aaagaccata catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc
     9961 aagagctatc cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat
    10021 ttcctgcttc aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac
    10081 tcaatagaac tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt
    10141 tctttttcat cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg
    10201 aagagttctt caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc
    10261 atcaagcatt ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga
    10321 tccaagccaa agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca
    10381 aacttcaacc cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg
    10441 acaaagtaaa ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt
    10501 ttcccttgcg ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt
    10561 tctcgattac tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca
    10621 ctaagcaatt ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa
    10681 tccgctactt gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca
    10741 tctgagaaaa gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata
    10801 ttttccgcaa ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt
    10861 tgattgcttt gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct
    10921 cgactagaaa ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc
    10981 tttttctcca taatcacata aggatcaagt aatccttgat tttttggctt actagttaag
    11041 ttgagaatat tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc
    11101 ccttttggat cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga
    11161 ttgttactaa aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg
    11221 gtaactgaat ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc
    11281 gtatctacgt tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca
    11341 aaaccaagtc cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc
    11401 ttactagaag gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt
    11461 tttacattgg tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct
    11521 actgattcgg cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga
    11581 tccaaggtag tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt
    11641 tcattgtcag attgataggc atggttgtcc aagtctttta attccttttt cttattgcga
    11701 acggtagcaa gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg
    11761 aatgtaaatt gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc
    11821 acaattgtat taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat
    11881 aagtaccggg gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc
    11941 accaaagttt ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt
    12001 gtaccctttt cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt
    12061 ctgactttaa atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg
    12121 cgaatttctt cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag
    12181 acaaattctt gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca
    12241 tctgtcgcaa tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc
    12301 atggatacta atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc
    12361 ccggtaattt ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt
    12421 tgtaaggcat gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg
    12481 acttgatatt tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag
    12541 gcaaaacatt ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac
    12601 atagttcaca ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac
    12661 tttctttcct gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa
    12721 tcccaatggt ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg
    12781 tatacctaag tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa
    12841 tacgatgaca aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa
    12901 aggcagttga aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta
    12961 gctttttatt ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact
    13021 acccttcaat aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa
    13081 ttgcctccaa tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt
    13141 tctggtactt ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata
    13201 tcatcgcaat ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa
    13261 ttgaagaaag catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc
    13321 tccgctaagc cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag
    13381 ggaagttcat aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct
    13441 ttcacttcat caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta
    13501 tatttcccga ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca
    13561 ccgacaattc ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc
    13621 aatccgaccg caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta
    13681 aatcctaaaa acaaaccaat gaccacaatt gccagtaaga ctaaggattg
//
LOCUS       DASJPC010000068        13597 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJPC010000068 DASJPC010000000
VERSION     DASJPC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.068"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..266
                     /gene="yaaA"
                     /locus_tag="U4A13_002002"
     CDS             24..266
                     /gene="yaaA"
                     /locus_tag="U4A13_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN1780278.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            253..1377
                     /gene="recF"
                     /locus_tag="U4A13_002003"
     CDS             253..1377
                     /gene="recF"
                     /locus_tag="U4A13_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN1780279.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1374..3320
                     /gene="gyrB"
                     /locus_tag="U4A13_002004"
     CDS             1374..3320
                     /gene="gyrB"
                     /locus_tag="U4A13_002004"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN1780280.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3340..5811
                     /gene="gyrA"
                     /locus_tag="U4A13_002005"
     CDS             3340..5811
                     /gene="gyrA"
                     /locus_tag="U4A13_002005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN1780281.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6047..6346
                     /locus_tag="U4A13_002006"
     CDS             6047..6346
                     /locus_tag="U4A13_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN1780282.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6396..6908
                     /gene="ssb"
                     /locus_tag="U4A13_002007"
     CDS             6396..6908
                     /gene="ssb"
                     /locus_tag="U4A13_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1780283.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6934..7170
                     /locus_tag="U4A13_002008"
     CDS             6934..7170
                     /locus_tag="U4A13_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN1780284.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7313..9286
                     /locus_tag="U4A13_002009"
     CDS             7313..9286
                     /locus_tag="U4A13_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN1780285.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9292..9744
                     /gene="rplI"
                     /locus_tag="U4A13_002010"
     CDS             9292..9744
                     /gene="rplI"
                     /locus_tag="U4A13_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN1780286.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10018..11385
                     /gene="dnaB"
                     /locus_tag="U4A13_002011"
     CDS             10018..11385
                     /gene="dnaB"
                     /locus_tag="U4A13_002011"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN1780287.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12165..13457
                     /locus_tag="U4A13_002012"
     CDS             12165..13457
                     /locus_tag="U4A13_002012"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN1780288.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4528 a         2465 c         2994 g         3610 t
ORIGIN      
        1 ataatgaaaa tgagggatgg gcattgaaac aacaagtcat tctaaatacg gaattcatga
       61 cgttgggcca aatgttgaaa gaagtcacag ttatcggtag tggcggacaa gcaaaatggt
      121 atttggcaga aaacactgtg ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac
      181 tttatcccgg catgatggtt gaagttccag aagtaggaac tttttttatg gtaaaaaaag
      241 gaaacgacga taatgaggct gaataagctg tatttaaaaa attatcgaaa ttacgaagag
      301 ctgaacatcg aattttccaa gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa
      361 acgaatcttt tggaaagtat ctacgtatta gctatgacgc gaagtcatcg gacaagtagt
      421 gagcaggaat tgattggctg gactgacgat caggcgatga ttcaaggaga gatcacaaaa
      481 ggttcatcca ctcttccatt agaaatctta ttatctaaaa aaggccgaaa aaccaagatc
      541 aaccatatcg aacagaaaaa gttaagtact tacgtgggac aaatgaatgt cattttattt
      601 gcaccagaag atctttcttt agtaaaaggc agcccgcaga ccaggcggaa gtttttagat
      661 atggaattag gccagatcaa tccaatctat ctttatgatc ttgtccaata ccaagcaatt
      721 ttgaaacaaa gaaaccagta cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt
      781 tatttggata ttttgtccga acaactagca gcgtttggca gcaaagtatt gaaagcaaga
      841 gcacagttcg tcaagcgatt ggagttttgg gctaattctt tgcatcagca aatcacccat
      901 caaaaagagc agctggaaat cgagtatctc accgcagtcg attctttaga aacacatacg
      961 caagagcaaa tccaagaaca atttctcgca cttttgaacc aaaataaaaa gaaagacctt
     1021 tttcggggaa caacgacagt tggtcctcat cgtgacgatc tttccttttt tattaatcag
     1081 aaaaatgtgc agacttatgg atcgcaagga cagcaaagaa caactgcact aagtgtcaaa
     1141 ttagcagaaa tcgatttgat taaagaagag acaggggaat atccaatcct tttgttagat
     1201 gatgtgatga gcgagctgga tgataatcgt caactccatc tgctagaaac aatcgaagga
     1261 aaagttcaaa cgttcttaac aacaacgaca cttgaccatg taaaaaataa aatgactgtt
     1321 gaacctgaga ttttttatgt tcaacaaggg aaactaaaag gagaagaaac tgaatgacag
     1381 aagaaagaag tttagtagaa cgcgctaaag agtatgatgc cagtcagatt caagtacttg
     1441 aaggacttga agccgttcgt aaacgacctg gcatgtatat cggttcgact agttctgagg
     1501 gtctgcatca ccttgtttgg gaaattgtcg ataattcgat cgatgaagta ttagcaggtt
     1561 ttgcaacgaa aatccatgtg attattgaaa aagacaacag tatcaccgtt attgacgacg
     1621 gtcgggggat acctgtagat atccaggcaa aaactggacg tcctgcagtt gaaacggtct
     1681 ttactgtttt acatgctgga ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac
     1741 ttcacggagt aggatcttct gttgttaacg cgctttctac acagcttgat gtcaaagtat
     1801 acaaagaagg aaatgtgtac tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa
     1861 agattatcga acaaacggat cgccatggaa caacggttca ctttacgcca gaccctgaaa
     1921 tttttacaga gacaactgag tttgattttt caaaattggc tacccggatc cgtgaacttg
     1981 ctttcttgaa ccgcgggatg cgtatatcga ttgaagacaa acgagaagaa gaaccagtca
     2041 tcaatgaata ccattatgac ggcgggatta aaagttatgt cgaatacttg aatgcaaaca
     2101 aaaccgtgat ttttccagaa cctgtatatt tggaaggtga acagcaagat atcgcagttg
     2161 aagtctctat gcaatataca gacggctacc attccaatat catgagtttt gccaacaata
     2221 tccatacgta tgaaggcgga acacatgaat caggattcaa gactgcttta actcgtgtga
     2281 taaatgatta tgcccgaaaa caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg
     2341 aagacgtgcg agaaggattg acagctgtta tttctatcaa gcatcctgac cctcaattcg
     2401 aaggacaaac aaaaacaaaa ttgggcaatt ccgaagtacg gacagtcact gatcggttat
     2461 ttagcgaaca tttcatgaaa ttcttgctgg aaaacccaag tgtgggccgt caaattgttg
     2521 aaaaaggatt actagcttcc agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga
     2581 gacgtaaagg tgctttagag attagtaatc tgccaggaaa attagcggac tgctctagca
     2641 acgatcctga aaaatgcgaa ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta
     2701 agcaaggtcg taatcgagag tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg
     2761 ttgaaaaagc aagcatggac aagatcttgg caaatgaaga gatccggtct ttatttactg
     2821 ctatgggtac agggtttgga gaagattttg atgtatcgaa agctcgctac cacaaattag
     2881 ttatcatgac tgatgccgat gtcgacggcg cgcatatccg tacattgctg ctgactcttt
     2941 tttatcgtta catgcgtcct attgttgaag ctggatacgt ctatattgct cagccacctt
     3001 tatacggggt aaaacaaggg aagaatatta cgtatgtaca accaggaaaa aatgcagaag
     3061 aagagttgaa acaagtcgtt gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt
     3121 ataaaggact tggagaaatg gatgatcatc aactatggga aacaactatg gacccaagca
     3181 atcggatgat ggcccgcgtc agtgtggacg atgctattgc agcagatcag atatttgaaa
     3241 tgttgatggg ggatcgagta gaaccccgtc gtgcattcat cgaggaaaat gcacactatg
     3301 tgaagaactt agatatttaa tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa
     3361 aacatccatg atgtcaatct aaccagcgaa atgaaagact ccttcatcga ttatgcgatg
     3421 agtgttattg tcgcacgtgc acttcctgat gtcagagatg gtctgaaacc cgttcatcgc
     3481 cgtattttat acggcatgaa cgaattaggt gtaacaccgg ataaagccca taaaaaatct
     3541 gccagaatcg tcggagatgt catgggtaaa taccatcctc atggagacta tgcgatctat
     3601 gagtcaatgg tacggatggc acagccattc agctaccgtt atatgctagt ggatggtcat
     3661 ggaaactttg gttctgttga cggtgacggt gccgccgcta tgcgttatac cgaagcacgg
     3721 atgagcaaaa tcgctacaga aatgctgcgt gatatcaata agaacactgt tgatttccaa
     3781 agcaactatg atgatacaga aaaagaacca gttgtactac ctgctcgctt cccgaatctt
     3841 ttagtgaacg gaacaacggg gatcgccgta ggtatggcca cgaatatccc acctcataac
     3901 ttgtctgagg tagtagcggc tatcgatttg ctgatggata atcctgatgt gacgaccaat
     3961 gaattgatgg aagtattacc tggaccagat tttccaacag gtggtttggt tatgggaaaa
     4021 tcaggaatcc gaagagcgta tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt
     4081 gagattactg agatgccaaa tggtaaagaa cggatcatcg tgacagaatt gccttatatg
     4141 gtcaataaag caaaattgat cgaacggatt tctgaactgc acagagacaa gcgtatcgaa
     4201 gggatcacag atttgcggga cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc
     4261 cgtcgtgacg ccagcgcatc tgttatcttg aataacttat ataaattgac atccttgcaa
     4321 aattcatttg gattcaatat gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg
     4381 aaacaaattc ttgaaaacta tatcgaacat cagcgcgaag tcatcacaag acggacagtc
     4441 tttgaaaaag aaaaagcaga agcaagggca cacattttag aaggtttgcg tattgctttg
     4501 gatcatatcg atgaaatcat tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa
     4561 gcgacgatga tcgaacggtt cgatttatcc gatcgccagt ctcaagctat tttagatatg
     4621 cgtttacgcc gattgaccgg tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc
     4681 ttaaaactga ttgaagattt aactgacatt ctggcaagac cagaacgtgt tacagaaatc
     4741 atcaagaatg aattagcaga cttgaatcaa cgatttggtg ataaacgacg cacagaatta
     4801 ctagtcggtg aggtcttaag tctggaagat gaagatttaa tcgaagaaga agaaatcgtt
     4861 attacattga caaacaatgg ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa
     4921 cgaaggggtg gacgtggtgt ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat
     4981 ctggtttctt gttctaccca tgatacattg ttattcttta cgaataacgg aaaagtgtat
     5041 cgggcaaaag gatacgaaat tccagaatac gggcgcacag ccaaaggaat accaattatc
     5101 aatttactag gtatcgattc gagtgaaagc atccaggcaa tcattgcggt gacgggagaa
     5161 gcagaagaag gccactatct cttctttacg acgcgtcaag gtacagtaaa acgtacttct
     5221 gtcactgctt ttgctaacat ccgaagtaat ggtctgatcg caattggttt gaaagaagac
     5281 gatgagctcg tcaatgtgct actgacagat ggtcaatcta atatcattat tggaacacat
     5341 aacggctact ctgtcacttt caaagaagaa gcagtccgcg atatgggacg tacagcttcc
     5401 ggggttagag gaatacgcct gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag
     5461 gaagatcaag aagtattagt gatcacagaa aaaggttacg gaaaacggac aaaagtatcg
     5521 gaatatccag tgaaaggccg cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa
     5581 aatggtccac tagctggatt gaccactgtt tctggagatg aagacatcat gctgattaca
     5641 gataaaggtg tcattatccg tttcaacgta tcgactgttt ctcaaactgg acgttcaaca
     5701 ttaggcgtac gtttgatgaa gatggaagct gatacgaaag ttgtcacgat ggcagcagtc
     5761 gaaccagaag ctgcagaaca aacagttgaa gaacaaggat cagaagaata gccatcaaga
     5821 ctaattgtgt aagctagcaa gaatgaccga ttaaaaaatc tggttattcc tgctggcttt
     5881 tttgattagt tgaatttctc ttaaaatatt cgaaactgtt cattctgtat tgcttttgac
     5941 caaaatagag tgtataatca gagcttgtga gtagtctaat taattagata actctccttg
     6001 ctcttaggaa cctaagagcc gaaagtccac agggaggtga atatcaatgg aaaacacgaa
     6061 atatgaaatc atgtacatta ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat
     6121 cgaacgtttc gatgcaatct tgaaagataa cggagcagaa gttatcgaat caaaagattg
     6181 ggaaaaacgc cgtttcgctt atgaaatgaa cggttaccgt gaaggtatct atcacatcgt
     6241 taaagtatct tctccatcat cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat
     6301 caacgacgat atcattcgtc acatgattgt taaagaagaa gcttaattgt tgtttcatgt
     6361 gaaacaatag aaaacgatga gaggagatga ctattttgat taataatgtt gtattagttg
     6421 gacgtctgac gaaagatcca gatttgcgtt acacagcaag tggtacggca gtcgcaacat
     6481 ttactttggc tgtgaatcgt aattttacaa accaaagcgg caatcgcgag gctgatttta
     6541 tcaactgtgt gatttggcgc aaatcagcag aaactttagc aaactatgct cgtaaaggaa
     6601 cacttttagg tgttactgga cgtatccaaa ctcgttctta tgataatcag caaggacaac
     6661 gtgtatacgt gacagaagta gtagctgaca acttccaatt gctggaaagt cgttctgcat
     6721 ctgaaaatcg ccaacaaagc ggcggattcc aaagttctgg tcaatcagct ggcggatttg
     6781 gcggaaacaa taactctaat caaacatctc aatcatccaa cggtatgcct gatttcgacc
     6841 gtgatacttc tgacccattc ggctcaagtt caacgatcga catttcagac gatgatttac
     6901 cattctaaac gaatttgata ggagggaaat agaatggcac aacaaagaag aggcggacgt
     6961 aaacgtcgta aagtagacta cattgctgct aaccatattg aatatatcga ttataaagat
     7021 atcgaattac taaaaagatt tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact
     7081 ggtacaggcg ctaaaaacca acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg
     7141 ggcttgctac ctttcgttag cgaagactaa taaatcctag gaattgaatg actgatcctt
     7201 ttgatcagct attcaattcc tttttttctg taaaaataac ctctattcgt ttagtcgctc
     7261 gaatgacgaa agttatggta aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa
     7321 taaaaacaat catttatccc cgaaacctct cttccttatc ctgtttatat taatcgaagt
     7381 attggtagtc ttaattttac catttcgttt tgttagcgct tcggtcattc tattgatgaa
     7441 tttatggggt gttcgctatt ggttgaaaat gaaacgttgg cttattttaa ataaacgtga
     7501 aaaaatccag caggcttcga ctgtagctga aaccaatcta gcttatgtct cagaagtcat
     7561 gcctgtagga gtgattgctt atcagccatc aagccaaaaa atcgaatggc tcaatccatt
     7621 agcattgaaa atcaaagatc aaagtggatt aacagaggaa gaattgctag atactttttt
     7681 tactgcaaaa gaaaaaaaga aaaaccaagt ctatctagca gataaaacat ttcagtttga
     7741 tatggataca gtaaagggtg ttgtttattt taatgatatt acccaaaacc agttgttaaa
     7801 actccatcag acgaacagtc agccggtgat tggtatttta tctattgata actatgcaga
     7861 tgccattgat aaattggatg acaaagagat ttcttatttg aactctttcg tcactacact
     7921 agtttctgac tggatgaatg aataccatgt ctttttcaaa agaatcaacg cagaacggtt
     7981 tttcttcgta gcacagatga tcgatctgaa aaagatgatg gataaaaaat ttgatttgct
     8041 ggatcgcttt cgagcagaag ctgaaaaaca gagctcgcct atcaccctta gtatggggat
     8101 tgcttatggc tcaagaaccc tcgatgaaat cggtggagaa gctcagaata atttagatgt
     8161 tgctttagtc cgtggaggcg accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc
     8221 tgtttattat ggggggaaat ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc
     8281 gatgagtacc gctttgcgtc ggatttttga tgaaacaggc gatgtgttta tcatggggca
     8341 caagtttccg gatatggatg ctttaggctc tgcttttggt gtcagttgtc tggcacgctt
     8401 caaccagaaa aaagcttaca tcgttatcaa cgagaacgag atcattccag atgtagaacg
     8461 gtgtcttgaa gaaatccata agcatcctga attagaaatg caactaatct ctccagaacg
     8521 tgccatagag ctgaaaaaag aagaggacct cctcgtgatg gtggattacc ataaaccttc
     8581 tctatcgatc tctcagggcc tatatgagca atttgataag attgtgatca ttgatcacca
     8641 tcgtcgtggg gaagagtttc caagtaaacc tctgctgact tatatcgaat cttcagcttc
     8701 ttcagcttca gagctagttt ctgagctgat ccaataccaa agcagcaaaa caaatcgctt
     8761 agatcgtttt gaggccacat tattgcttgc agggatggtt gtagatacaa atagtttcaa
     8821 tgtccgtaca actgcacgaa cttttgacgt tgccagctat ttaaggtctt gtggagcaga
     8881 cgcatcctta gtacaatatt tattgagctc ggatttagcg acatacttgg agatcagcaa
     8941 tttgattgct ggaagcgact atgtgacttc tgatattgtt gtggcggcag ggacagacga
     9001 aaaagagtat aatagtgtta tagcagcaaa aacagcagat acattgcttt cgatgatcaa
     9061 tatcaatgca gctttcgtca ttacaaagcg gacagacggc ttgattggga tcagtgccag
     9121 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
     9181 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
     9241 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
     9301 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
     9361 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
     9421 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
     9481 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
     9541 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
     9601 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
     9661 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
     9721 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
     9781 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
     9841 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
     9901 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
     9961 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    10021 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    10081 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    10141 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    10201 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    10261 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    10321 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    10381 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    10441 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    10501 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    10561 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    10621 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    10681 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    10741 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    10801 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    10861 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    10921 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    10981 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    11041 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    11101 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    11161 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    11221 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    11281 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    11341 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    11401 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    11461 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    11521 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    11581 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    11641 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    11701 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    11761 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    11821 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    11881 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    11941 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    12001 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    12061 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    12121 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    12181 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    12241 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    12301 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    12361 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    12421 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    12481 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    12541 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    12601 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    12661 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    12721 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    12781 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    12841 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    12901 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    12961 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    13021 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    13081 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    13141 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    13201 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    13261 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    13321 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    13381 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    13441 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    13501 gtcgaagtgt ttatctatac gataataata acttgaacac tgtttattca gaataaggga
    13561 ctgtgacaaa aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DASJPC010000069        13435 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJPC010000069 DASJPC010000000
VERSION     DASJPC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.069"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..249)
                     /locus_tag="U4A13_002013"
     CDS             complement(<1..249)
                     /locus_tag="U4A13_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1780289.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLI"
     gene            complement(728..1447)
                     /locus_tag="U4A13_002014"
     CDS             complement(728..1447)
                     /locus_tag="U4A13_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN1780290.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1496..3142)
                     /locus_tag="U4A13_002015"
     CDS             complement(1496..3142)
                     /locus_tag="U4A13_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1780291.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3321..3911
                     /locus_tag="U4A13_002016"
     CDS             3321..3911
                     /locus_tag="U4A13_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1780292.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            4003..5508
                     /locus_tag="U4A13_002017"
     CDS             4003..5508
                     /locus_tag="U4A13_002017"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN1780293.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5592..6944)
                     /gene="celB"
                     /locus_tag="U4A13_002018"
     CDS             complement(5592..6944)
                     /gene="celB"
                     /locus_tag="U4A13_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1780294.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6944..7462)
                     /locus_tag="U4A13_002019"
     CDS             complement(6944..7462)
                     /locus_tag="U4A13_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780295.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7478..7804)
                     /locus_tag="U4A13_002020"
     CDS             complement(7478..7804)
                     /locus_tag="U4A13_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN1780296.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7817..9796)
                     /locus_tag="U4A13_002021"
     CDS             complement(7817..9796)
                     /locus_tag="U4A13_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN1780297.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9817..10131)
                     /locus_tag="U4A13_002022"
     CDS             complement(9817..10131)
                     /locus_tag="U4A13_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN1780298.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10299..10592
                     /locus_tag="U4A13_002023"
     CDS             10299..10592
                     /locus_tag="U4A13_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN1780299.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10670..11782)
                     /locus_tag="U4A13_002024"
     CDS             complement(10670..11782)
                     /locus_tag="U4A13_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1780300.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11935..12159
                     /locus_tag="U4A13_002025"
     CDS             11935..12159
                     /locus_tag="U4A13_002025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780301.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            12169..12348
                     /locus_tag="U4A13_002026"
     CDS             12169..12348
                     /locus_tag="U4A13_002026"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780302.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12542..12712
                     /locus_tag="U4A13_002027"
     CDS             12542..12712
                     /locus_tag="U4A13_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1780303.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13136..>13435)
                     /locus_tag="U4A13_002028"
     CDS             complement(13136..>13435)
                     /locus_tag="U4A13_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1780304.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4260 a         2698 c         2286 g         4191 t
ORIGIN      
        1 gatgagaaat gcttgatccg aagaaaataa aaatttgaaa ttttcccaac ccacccaagg
       61 actttccctc aagctagcca agaacccatc tgctgaaata tgaaaatttt tgaatgctac
      121 gacattcgcc attacaggta tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc
      181 catcaggaca agtgctttat aacgccatag attcataaaa aaatgattga acccctttcg
      241 ttttttcatc taaaaacccc tttcgattca ttcgcaacgt aattatagcg ctttcaataa
      301 tgagaataaa taaacaaagt atagggaaaa tgaaacaaag tataggagag gttgtgaaat
      361 cagcgttttg gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg
      421 ttttttgagc taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaatt
      481 cagcgttttg gcctgagtat tagggagatc gtgtccttac tatctaactc tgagtcacaa
      541 ggaacaatgg ggactgtaac aaaagtcttg tcacagtccc ttattccgaa caaacggtgt
      601 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaaagccat ccgaaagtca
      661 gacggacaag tctgcttttc agatggcgct acaactactc tgcttattct tttagctatc
      721 cattcaatta tttttctctc acttgttcta gctcttcttt ggtcatgggt cgatattgcc
      781 ctttattcaa atctttaggc agccaaaatt ttcccatacg gattcttttt aaatataaga
      841 cttcttttcc tactgctttg accattcgct taacttggtg aaactttcct tcatgcaaaa
      901 tcagacggat aattgaagtt tctgtctctt gatctacttg atcaatttgg agttttgccg
      961 gcaatgtctg ctcatcgcca tcaataatca gtccttctgc aaataaacgg atatcttctt
     1021 ctgtcattat accgctaacc tcagctagat attctttctc cacatgtttt ttaggtgata
     1081 gtaactgatg ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat
     1141 ctaatcggcc gactggaaaa agatcttccc gataatcctg gtcttccaat aaatcgatta
     1201 cggtttgatc atgattatcc tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt
     1261 agtaaaaatc tttttgatac gtaatgattt ctcctaaata agtaaccgca tcttcgaatt
     1321 catttacttg cgctttatcg cttttcacca cttgtccatt gacttcgatc gttccttttt
     1381 tgattaatcc tttgacttct ttgcggctgc caagtcccat atctgctaaa aacttatcta
     1441 aacgcatgcg tttcccccat ttcaaaagag catcaatcta gctcttctga ttcacttatt
     1501 ttatttttaa tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc
     1561 ggattttcaa agctaaataa ccatacacta aagcccctat acctgctact acaagaatca
     1621 acaatagaga ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga
     1681 tccacgaagc caaaagcata atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga
     1741 caaaacttgg tttaaagcgg gctacttcat ggattctctt taagatcaag gtacaagaca
     1801 cagcaaatcc gatcattgtc gccaataaag ggccgtatac ttcaaataaa cgaatggctg
     1861 ggtattgaag aaggagtttc actagaaacc caataaccaa ataattaatt gccgccttgt
     1921 tttcaaacat tccttgcagc atgttggaaa ctagcatata tagtcctagg aagagtccta
     1981 caaaacttgc ttggatcagt acattacttc ctagagaatc cggtgtataa aataatgtat
     2041 ttagcggata ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca
     2101 actgtaaatt tccgcttgtc aattttgcca gacctactcg atctttcaac gtaacagctt
     2161 ctgtaatcaa cggtaaccca gtagacgcga tcgatgttgc caatgcaata actaccatcg
     2221 tcaacttatc tggattggca ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat
     2281 actcggtgga tgcagacatg atcttcataa aagtgaactg atcgactaat ttgaagatcg
     2341 ttacccctga acccacaata ataaacggaa tcgcttcttt aacggtatct aacaacaatt
     2401 ccttcgttgc aatatttatt ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt
     2461 gatgtttctt caaaaaatac aaaaggacaa gaatacttgc tagcataccg ataaatgccg
     2521 cgaaagttga ttgcgtgacc gccgtcacat agtcaccatc catgactttc atgatgatga
     2581 atgtagccaa tagcatataa aaaatacgtg ccacctgttc tacaatctgc gacagcgcat
     2641 aaggcatcat ttcttgattg ccttgcaaat accctcgtat cacactcata caaggaaaaa
     2701 ccaatacagc gatactcaaa gaacgcatcg ttggaatcag ttcttctccg ccaccagaag
     2761 catgagctag ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc
     2821 ccatcatctg taatgcacgg ataaataatc gtcgtgaagt gccatattca ttcaatgaat
     2881 tatagcgtgc agtttgtttt gcaatcgcag caggtattcc tgctgtagag atcagtagaa
     2941 acaatgcata tatattgtat cccatattga ataatccatt ggcagcctta gcattttcgc
     3001 ccatccaagc ataccaggga atgatataga cagcgcctag caatcttgaa atgatattac
     3061 tggcggtcat ccaagcagag cctcgtgcca ttttttcttg cggtgttaat tgatctaatg
     3121 ctggttttct ttgattatcc atcggtcaga ttccaacttt ctacaaaaca ttactatcat
     3181 tttaattggt aaaacagaaa ctggcaatcg attttatata acttttataa gaacaattgt
     3241 ttttttgaat tcggtttcgt ttttttggta aagtaaagaa atatacagcc ttttatggct
     3301 aacatttata ggagatatca atgacacaag aaataaaaaa actacccgtt atatttttag
     3361 gtcttttctt tatcacagtt ggattgaatt ggttcttgct ccctcacgat atagcttccg
     3421 ccggagtggg cgccatcgga catttagttg aaacggcctt ttctatccaa cgctcagtta
     3481 ctgtttggag catcaatctt atgatgctgc ttctagccgc tgtcctatta ggaaaatcag
     3541 tattttttaa gactgtagcg ggaagtctgc tttttcctgt tgttttagag attgttccaa
     3601 aagtggaaat gatctcctca catttctttt cactgatttc aggaagtctt ttgttcagtc
     3661 ttggggttta cacattgtac acagtcgggg cctcaaatgg cggtataacg ataccgccga
     3721 ttattttcca caaatttttt cggcttccaa tgccgcaagg acttttactt accaatagtc
     3781 ttatcgtctt tttgaattat tatgtttttg gattgttgga gacactattc gttctcctat
     3841 ctattacagt cagttcattg tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa
     3901 aatttgaata aagctggatt ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg
     3961 ctataataaa ccaagaaatt gaataaagcg agggatcaat caatgagctt ctctttagaa
     4021 gacatccgtc atttactatt aaaagaaaat ctattaaaag aatttgtttc aacaagaggc
     4081 tggcatcttg atgtaccaga aaaagctgaa tttcttcagt taagttatga ttcaagaaaa
     4141 gctgatgctt ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga atatttggat
     4201 tcagcaatcg aacagggtat tcaatattat gtatcagaac aaccatacga aaattcagca
     4261 gtaggaatca tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat ggctttttac
     4321 gactatccgc aaaataaatt gaaagtgatc ggattcactg gaacaaaagg taaaacaact
     4381 gctgcgtact ttacaaaagc aatcttggat cacacaacaa ataaaaaaac agcattgctt
     4441 tctactatga atacaacgtt agatggcaaa acatatttca aatcccattt gacaacgcct
     4501 gagtcattag atttatatcg tatgatgtca gaagcagttg aaaatggtat gactcatcta
     4561 gtgatggaag tttcttcaca agcttacaaa acacaacgcg tatacggctt gactttagat
     4621 gtcggaatct tcttgaatat ctcgcctgac catatcagtc cgatcgaaca tccgacattt
     4681 gatgactatt tctattgcaa gcgacaattg atcttgaatt caaaaacagt tgttttaaat
     4741 catgaaagtg actattttga tttacttagg gaaacagccg atctattcca gattccaacg
     4801 atcacttatg gtcgttcgga agaaagcaat tatcaagtga tccgttcaga taaaggtact
     4861 cacggtttta ctttatcttc tcatgaagat cgcttagcta tctgcgatac tgcttatgat
     4921 attttgctgg ctggaggatt caaccaagaa aatgcagcca gtgcgatcat tgccgcagca
     4981 ctaacaggtg cctccagtca agatgcacaa gacggattaa aagaagctcg tgtaccaggc
     5041 cgaatggatc aattgatcca atccaatgga gcgcatgtct atgtggacta tgcacataat
     5101 tatctaagct tgaaaacatt gctgaaattt gccaaaaatg aacatccaga tggccgtgta
     5161 attgttgtat taggcagccc aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa
     5221 gtgctatcag aaacagctga catcgccttt ttgacggcag atgacccagc atttgaagat
     5281 ccgaaaaaaa tcgcggaaga aatcaatgaa gcaattacta atccagattt gatcgttcat
     5341 tatgaaatgg atcgtcctga agcgattcga cgcgctttag cagaaagcgc tccgcaagat
     5401 tcagtcgtta ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa
     5461 ccttatgaag gggattatgc aatcgtgaag cggttgattg aagaataatg gtatttatat
     5521 actagtggtt tcccatttct aagaaaaaaa gggactgtga ttttttgtca cagttccttt
     5581 tttcttagaa atcattttaa cttttcacta gctaattctg ctgccttttc tctttcttca
     5641 tttaataatt tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt
     5701 gcacaaatga ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg
     5761 atcccgactg gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga
     5821 atggcaaaat aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa
     5881 tagggattgt agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct
     5941 ggtacaagtg aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata
     6001 aagatgatca atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat
     6061 tctcctgcca acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc
     6121 ggggtcaaga aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg
     6181 atcaagaaat aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca
     6241 aatgggatag caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg
     6301 atcatcacca caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact
     6361 cctggcggaa caacatctgg catttttatg acccaatttc tcttgacaca catacaatac
     6421 aattcgaccg caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt
     6481 tcgacaaaag agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca
     6541 atcaagctga ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa
     6601 acagatagta atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt
     6661 gacgttaatt cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt
     6721 gtattgacga tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct
     6781 aaaaatggga acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg
     6841 aaaggaatag aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt
     6901 ttccccatag gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc
     6961 ctaaatataa cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg
     7021 aaattttctt taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa
     7081 tgaaaacaaa taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc
     7141 ctgaggtaaa caacactgga atcactaata aattaaagac actctggaac aaaaaatacc
     7201 actttgtcca tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg
     7261 caggcaaaat agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata
     7321 gaagcagaat catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga
     7381 ttagataacg attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt
     7441 tcctttttac tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat
     7501 aattcatttc ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat
     7561 ggaccatgat aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag
     7621 tttgtgactg atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt
     7681 cttcaaacat ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc
     7741 cgctatggaa aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag
     7801 ccatctgttt tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa
     7861 taggttcagc taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt
     7921 tagtcacgac cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat
     7981 gaatttcttg atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg
     8041 tttcgattcc aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt
     8101 gtcccatatt ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt
     8161 catttatgct cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg
     8221 tataagtctt gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta
     8281 gagaaaatgc tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct
     8341 ttcgtatttt acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt
     8401 tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg
     8461 tttcattgat ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac
     8521 tgactacgcg attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg
     8581 catgtagttt atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat
     8641 aggcccattc atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc
     8701 tttttactgc gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct
     8761 caatgcgaat acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat
     8821 tgaaaagctg gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa
     8881 ctttcgctaa ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag
     8941 ccatgagatg taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta
     9001 cctcaatacg ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt
     9061 gttcgatgcc taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca
     9121 agtgtaaagt cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt
     9181 catcgagaat gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc
     9241 ctaaatattc tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat
     9301 gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt
     9361 ttagtgaata tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac
     9421 gactgatata aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca
     9481 attggttcaa tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag
     9541 catatccagg tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa
     9601 tatgcagttg gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta
     9661 actctttgat ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag
     9721 ctaaacgttg actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt
     9781 tttccttctg tttcattatc atccactcct attccatcat atgttctctt cgatcacctc
     9841 tgctaacttc tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac
     9901 aggctttcca acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg
     9961 gctgatcaga aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga
    10021 aactgcatct acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc
    10081 catcatcgag gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc
    10141 actcctttta tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata
    10201 tttccgtagc attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt
    10261 gatatactag tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt
    10321 tgtatacaag tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca
    10381 ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag
    10441 aagatccaga atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc
    10501 atcacaaatc cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa
    10561 tgaaagtcga acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt
    10621 atcatccatg tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca
    10681 aaccgtgttt ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc
    10741 ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga
    10801 ataaactggc agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg
    10861 tagaacaaac acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt
    10921 ggctgatacc aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc
    10981 gttctttgaa accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc
    11041 ctgcccacat caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc
    11101 caaatgcata gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct
    11161 ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa
    11221 accaacaaaa aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg
    11281 aatctttagg taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc
    11341 ccattttggg atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca
    11401 acatgcccat aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca
    11461 tgacgattgc taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa
    11521 tactcatcaa catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg
    11581 ttgtaattat aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca
    11641 atgaacaaat atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta
    11701 aaataaacgg atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt
    11761 tacttttatt ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc
    11821 gttgtcattt tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt
    11881 ttcttgtatg attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat
    11941 ggttctatca aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct
    12001 tacgaaacct tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca
    12061 tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg
    12121 aaaaacaaga agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt
    12181 atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa
    12241 agttatttta tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct
    12301 ttactgactg aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca
    12361 accgttattc gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt
    12421 ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga
    12481 gttttatttc cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa
    12541 aattcctcga ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt
    12601 agttacagac atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa
    12661 attggtgatt gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt
    12721 cacgagttgg ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca
    12781 aagagccatt aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa
    12841 atcaaagtat tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa
    12901 tgattttcgc agtgtttttg tatatttttt attaactagt attactacaa aggtttattt
    12961 ttcacttctc aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc
    13021 tctactccga tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa
    13081 ttgatgggta ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata
    13141 tatttcgttt ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca
    13201 atataaactg gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg
    13261 ttggattgtt tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa
    13321 agatcgccac ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat
    13381 accccgttgg cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatc
//
LOCUS       DASJPC010000070        13398 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJPC010000070 DASJPC010000000
VERSION     DASJPC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.070"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(14..706)
                     /locus_tag="U4A13_002029"
     CDS             complement(14..706)
                     /locus_tag="U4A13_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1780305.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="U4A13_002030"
     CDS             complement(1143..1712)
                     /locus_tag="U4A13_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1780306.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="U4A13_002031"
     CDS             complement(1786..2109)
                     /locus_tag="U4A13_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780307.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="U4A13_002032"
     CDS             complement(2263..3075)
                     /locus_tag="U4A13_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780308.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="U4A13_002033"
     CDS             3429..4277
                     /locus_tag="U4A13_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN1780309.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="U4A13_002034"
     CDS             4422..5363
                     /locus_tag="U4A13_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN1780310.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="U4A13_002035"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="U4A13_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="U4A13_002036"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="U4A13_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN1780311.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="U4A13_002037"
     CDS             complement(9827..12013)
                     /locus_tag="U4A13_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN1780312.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="U4A13_002038"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="U4A13_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN1780313.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="U4A13_002039"
     CDS             complement(12235..12675)
                     /locus_tag="U4A13_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN1780314.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="U4A13_002040"
     CDS             complement(12749..13288)
                     /locus_tag="U4A13_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1780315.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DASJPC010000071        13380 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJPC010000071 DASJPC010000000
VERSION     DASJPC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.071"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            175..1200
                     /locus_tag="U4A13_002041"
     CDS             175..1200
                     /locus_tag="U4A13_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN1780316.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1375..2619
                     /locus_tag="U4A13_002042"
     CDS             1375..2619
                     /locus_tag="U4A13_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN1780317.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            2772..3719
                     /locus_tag="U4A13_002043"
     CDS             2772..3719
                     /locus_tag="U4A13_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1780318.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            3853..4482
                     /gene="upp"
                     /locus_tag="U4A13_002044"
     CDS             3853..4482
                     /gene="upp"
                     /locus_tag="U4A13_002044"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN1780319.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4621..4863)
                     /locus_tag="U4A13_002045"
     CDS             complement(4621..4863)
                     /locus_tag="U4A13_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780320.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            4984..5056
                     /locus_tag="U4A13_002046"
     tRNA            4984..5056
                     /locus_tag="U4A13_002046"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5017..5019,aa:Thr,seq:cgt)
     gene            complement(5175..5813)
                     /locus_tag="U4A13_002047"
     CDS             complement(5175..5813)
                     /locus_tag="U4A13_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1780321.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(5936..6568)
                     /locus_tag="U4A13_002048"
     CDS             complement(5936..6568)
                     /locus_tag="U4A13_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN1780322.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            6919..7218
                     /locus_tag="U4A13_002049"
     CDS             6919..7218
                     /locus_tag="U4A13_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1780323.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            7713..9878
                     /gene="acm"
                     /locus_tag="U4A13_002050"
     CDS             7713..9878
                     /gene="acm"
                     /locus_tag="U4A13_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN1780324.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(10150..10635)
                     /locus_tag="U4A13_002051"
     CDS             complement(10150..10635)
                     /locus_tag="U4A13_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN1780325.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    10725..11070
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11115..11756
                     /locus_tag="U4A13_002052"
     CDS             11115..11756
                     /locus_tag="U4A13_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN1780326.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            11775..12410
                     /locus_tag="U4A13_002053"
     CDS             11775..12410
                     /locus_tag="U4A13_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN1780327.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            12428..13243
                     /locus_tag="U4A13_002054"
     CDS             12428..13243
                     /locus_tag="U4A13_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN1780328.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         4663 a         2178 c         2676 g         3863 t
ORIGIN      
        1 tttatttgtt tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg
       61 gtttatcaac aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt
      121 gaggataaac tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa
      181 acgaaacgat atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa
      241 cttgttgctt ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac
      301 gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat
      361 gtcgcctctt ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag
      421 attgtgaaga acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact
      481 ttgccctcag ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa
      541 aaaacactag aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg
      601 tcgggaaaac caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt
      661 accgggatta ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta
      721 agtgatccaa cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc
      781 gaagcagtca tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca
      841 ccaaaagccc ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc
      901 agagatggcg attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt
      961 gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg
     1021 tataacgaca actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta
     1081 gatgaaccaa cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg
     1141 gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga
     1201 tcaattggtt attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat
     1261 cagtgaacga aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat
     1321 ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta
     1381 gattacaaaa cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa
     1441 gaacataatt tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca
     1501 caaggaagta tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt
     1561 gggtgtgaat ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc
     1621 ggagcgaaat ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat
     1681 ctcgctttgg tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat
     1741 ctaacacatg gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt
     1801 gttgatccga caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat
     1861 caaccaaaat tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa
     1921 ttcagagaaa tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca
     1981 ggattagtag cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact
     2041 accactcata aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca
     2101 ttagcaaaaa aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat
     2161 gtgattgcgg gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat
     2221 agtgaacaaa tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga
     2281 accagagtca tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta
     2341 ggtataaatg gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa
     2401 aactcgattc cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg
     2461 ccagcaatca ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt
     2521 gtcaaagcat tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta
     2581 cgcgaattaa ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa
     2641 acagaaaagt tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca
     2701 atatcggtga tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg
     2761 gaggaaaagt catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg
     2821 caaatgcgcc tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag
     2881 aagaagtaca agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg
     2941 atactggcga atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata
     3001 aattaccagt tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc
     3061 atgataaatt gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat
     3121 atagtcgatc accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc
     3181 aacagctgaa taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag
     3241 gagattcagt aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg
     3301 gcctcccaat atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag
     3361 attcctatca tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt
     3421 gggaccaata tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag
     3481 caaatataaa tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg
     3541 tattaagaga tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta
     3601 cacaagtacg cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga
     3661 caaaggcaac gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag
     3721 ctttgcttgt ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga
     3781 gtttgactag tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg
     3841 agagcttaaa aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta
     3901 actattattc gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc
     3961 gctatgttga tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa
     4021 acaccgatca ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca
     4081 atcttgcgcg caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa
     4141 gtgggacata tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc
     4201 aaacttccag aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca
     4261 ggcggatcag caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa
     4321 tttgtttgtt tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat
     4381 attgacatct atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct
     4441 ggactagggg atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt
     4501 gtgacacaac ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct
     4561 ttttcacagc tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat
     4621 tcaaatcttt tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa
     4681 gttcgcattt tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga
     4741 aatcgttcca gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa
     4801 tttttcacca atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt
     4861 cataaaacct gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa
     4921 actaattttc catcggactc tttacaatca gccaaataga gtatataatt tcttttggat
     4981 aaggccagct tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg
     5041 atccctatag ctggcattaa aagagaagac agatatatca aggattttta atccattgat
     5101 acatctgtct ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt
     5161 atttctataa aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc
     5221 acatgaactg cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac
     5281 aatcttgcca ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta
     5341 tcataagtac tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga
     5401 atgctgaact tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt
     5461 atcatgatag gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct
     5521 gttaaacccc atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca
     5581 ttgataaaaa aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt
     5641 ttcgtatctt ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa
     5701 aaaatgctaa taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata
     5761 gtgcccaata tccctttttt aagttctatt ttttttccta aaatgttatt catgataata
     5821 actcctacta ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa
     5881 tgttttttta tggatatttg tattgctaaa aatttatctt atatattcat tttgattata
     5941 ctacttttat ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac
     6001 aattttttag atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga
     6061 tattttttaa atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta
     6121 ttttagtgag ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac
     6181 tttcttttat gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg
     6241 tgttcccaat ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca
     6301 cgcgatgcaa catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac
     6361 agtcggtaat ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag
     6421 atttgtaaat aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga
     6481 tattatacag ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc
     6541 ttgcttcatt aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat
     6601 taacaaaagc taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga
     6661 taacgatagt gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta
     6721 ggcgtaattc taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt
     6781 ttaaaataaa aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat
     6841 ttaaaaaaat ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat
     6901 ttttgggagg aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta
     6961 tatgatctcg ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca
     7021 aagacaggct tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt
     7081 ttacgtccgt tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag
     7141 atatcaacaa taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt
     7201 acttttgggc atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat
     7261 ctcataatgc aggctaggtt catttatttg aacagagtct gtattttttt attctaaata
     7321 aagaaacaga tgtttcttta tattgtattt tatcctttaa ttaacacaaa aacagctagt
     7381 ataaactaat aatgttaatt aaaagtgtat tttatgaaac tatcgttatt tattaatgtt
     7441 gcatttttgt aagttaaaaa ttggaaatat agattgaaaa aatgaaagca ataagtaaaa
     7501 taaaatgaat atatgcgaaa aaataattta cattaggaaa aaccttgtaa aaacagtttt
     7561 ttggcatatt tattttttat aaatatttgt aaaataacta tcaatttttt agttaaaaga
     7621 ttggaaatga gtgggggagg aattgaagaa aggatttttg agagatgata tagtaggttt
     7681 tatttttagt taaaataggg gagatagaaa aaatgaaaaa gtgtagaaat attattttat
     7741 caatgttatt tattattact aatataacat cactgattcc tgttcatgtc tatgcggatg
     7801 caggcagaga tatcagcagt aatgtcactt cgttgaccgt ggacccaacg aatattacag
     7861 atggaggaaa cataaaagta aaattctcgt ttgacgaaaa aaaacaaaat atccaaccag
     7921 gtgattatct ttggatcaat tggccaagtg aaggaaatat tcgcggtgaa ggattccaaa
     7981 aagaaatccc attaatgatt gaaaataaaa atgttggaac tttaacggtc agaaaagatt
     8041 ctgcgcaggt tgtgtttaat gaaaatatta aaaacctaga ctcagtagaa ggttgggggc
     8101 aatttgagat acaggccaga aacgtaaccg ataccggtga agaaaatacg ggaccattca
     8161 ccgtgacgag cggtgataaa acagctactg taaatgtgac aaagccagct tctggttctt
     8221 cttctagcgt tttttattat aaaacaggag acatgctacc tgaagatact aagcacattc
     8281 gatggttttt gaatattaac aataacggca cttatgttga acaaccagtt aaaatcagtg
     8341 atgaaatcca aagtggacaa agattagatc caagtacgtt tgaaattaat caaatacact
     8401 taggtgaaca gaaggtttat agaggagaag agggaatcca acaattttta caagatttcc
     8461 ccagcgctac atttaatttt tcagtaactg ataattacat tgaaataact ataccaaaaa
     8521 actttgtcaa tcttaggaaa ataatggtga gttataagac gatcatagaa aacccagaac
     8581 aaataaattt tgagaatcat tcggaagctt ggtttaaaga atttaataaa cctgctgttg
     8641 atggagaaag tttcaatcat acagttaaaa atatttcagc ttctggtgga gtcaacggta
     8701 cagttagagg cgaattgaag attttcaaat atatcaatga cacagagatt ggtatcccaa
     8761 atgtgacttt cgaattaaga cgagcagatg aacagcctat tcaaggacag tctagtatcc
     8821 tgttgacttc gaatgaacag ggagaagcta gtatcaaagg cctgcaagta ggagactatg
     8881 ttgtaaaaga aaaagaagca cccaattgga ttgattttga tccactaagt tcaaatgaac
     8941 tcaaattctc cataaacgaa aatgatacag agggagtcag tttacctatt tataacaaga
     9001 aaaaggttac gaacatcaca gcaactaaaa tttggaacgg tggaaccact ccaagaccga
     9061 gtatttattt taaattattc agagcaagta caaacaattg ggaaccagtt cctgatgcag
     9121 aaacaaaacg attagacaat ggaataactt cagtgacttg ggaagatatc caacagtatg
     9181 atgatagtgg aaacgaatat acgtttaaag tgcaagaagt agatcaaaac ggaaatgatt
     9241 atgtcccttc aggttatcaa aaaattgaaa acgggctagt cgttacaaat gagaataaag
     9301 aagtcatttc agtctcagga caaaaaacat gggaagacaa tgagaaccaa gacggcaaac
     9361 gaccgacgac gatcacagta aatctattag ccgacggtca accgatccaa cacaaagaag
     9421 taagtgaaaa agatgattgg cgctatcgtt tcacaaatct gccaaaatac agagacgggc
     9481 aagaaatcat ctatactgta acggaagaca acgtgcccga atacagtacg acgattgacg
     9541 ggtataacat caaaaacagt tatacaccgg aaaaaacaaa tatatctata ccaggtagcc
     9601 atataacacc gtggaaaaat tttccagaga agacagagag agagaaaatt agaaatctat
     9661 tctctcaaaa attgcaaata tttgctcata caggagaatc cagcgaacaa agaagcaatt
     9721 atattagtaa gagagctgta cctaaacaga taacaaataa atcaaaaagc attcttccta
     9781 aaacaagtag taaaaaatct agttttgctg taatcattgg tttattgata gtaactatct
     9841 gtgctgggat attcttacaa aaatataaaa aagtttagtt agaatataat attttttgtt
     9901 aaatatgact gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa gaagcatttt
     9961 tatatatgct tcttttttcg tagaatcaat aaatatggat ttggctaact atggaagaat
    10021 actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg ccaaaagaat
    10081 ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa taagaaaact
    10141 actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa gtatcttttc
    10201 aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa agcctacggt
    10261 atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt tgtcgtgcat
    10321 caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa gctgttcttc
    10381 agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta aatcggcgtg
    10441 cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt tcaataatag
    10501 attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg taatcgcagg
    10561 atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag cttcttgttc
    10621 ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat aaaaaagagt
    10681 tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg acatcgaaaa
    10741 gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag gcagcagtta
    10801 accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa tccggggagc
    10861 ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc gtgaggagtc
    10921 ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca aaggactttt
    10981 tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa aaataaacag
    11041 aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt tttattttaa
    11101 aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta gtaggagcac
    11161 tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta gggattgttt
    11221 tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa acatatgtat
    11281 gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat gggttgccga
    11341 cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc ttaaactatg
    11401 cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa ggtcaatatg
    11461 aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc gtcatcccac
    11521 aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg gtcaaagatt
    11581 cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa atcgcgatgg
    11641 aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt acattgactg
    11701 gtattctaac aatcataatg aaacaagttg aaggaaaatt aaattattac cgctaactga
    11761 taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt gaaaaaaaaa
    11821 taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc gtaggtcctt
    11881 ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt tctgacagcg
    11941 gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg aaagaacaag
    12001 ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt tttgaaaaca
    12061 ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc aaaaaagcag
    12121 aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct tatcagcttt
    12181 ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat ccacgggtat
    12241 tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa gtcgagcgtt
    12301 tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat gatttagtat
    12361 ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa gaaaggagaa
    12421 gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc ttctttttgc
    12481 aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg aagagaaaaa
    12541 aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata aagacgggaa
    12601 gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg ggattacggt
    12661 agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg gaacgatcga
    12721 tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc aatttacaaa
    12781 accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa tcacaaactt
    12841 tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt atgatgtatt
    12901 tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg ttttgtatga
    12961 tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc tgctgatgga
    13021 taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact tcaacataac
    13081 aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag atagtacatt
    13141 agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat tccaaaaaat
    13201 atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag gggactgtga
    13261 caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc aactccttgt
    13321 gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa aagccagttt
//
LOCUS       DASJPC010000072        13103 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJPC010000072 DASJPC010000000
VERSION     DASJPC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.072"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            52..1791
                     /gene="yfcC"
                     /locus_tag="U4A13_002055"
     CDS             52..1791
                     /gene="yfcC"
                     /locus_tag="U4A13_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1780329.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1948..3132
                     /gene="iadA"
                     /locus_tag="U4A13_002056"
     CDS             1948..3132
                     /gene="iadA"
                     /locus_tag="U4A13_002056"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN1780330.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3243..3446
                     /locus_tag="U4A13_002057"
     CDS             3243..3446
                     /locus_tag="U4A13_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN1780331.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3547..4209)
                     /locus_tag="U4A13_002058"
     CDS             complement(3547..4209)
                     /locus_tag="U4A13_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1780332.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4222..5724)
                     /locus_tag="U4A13_002059"
     CDS             complement(4222..5724)
                     /locus_tag="U4A13_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1780333.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6272..7951
                     /locus_tag="U4A13_002060"
     CDS             6272..7951
                     /locus_tag="U4A13_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1780334.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8078..8557
                     /locus_tag="U4A13_002061"
     CDS             8078..8557
                     /locus_tag="U4A13_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN1780335.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8776..9456
                     /locus_tag="U4A13_002062"
     CDS             8776..9456
                     /locus_tag="U4A13_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN1780336.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9464..10795
                     /locus_tag="U4A13_002063"
     CDS             9464..10795
                     /locus_tag="U4A13_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN1780337.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10810..11772
                     /locus_tag="U4A13_002064"
     CDS             10810..11772
                     /locus_tag="U4A13_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN1780338.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11883..12221
                     /locus_tag="U4A13_002065"
     CDS             11883..12221
                     /locus_tag="U4A13_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN1780339.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12300..13007
                     /locus_tag="U4A13_002066"
     CDS             12300..13007
                     /locus_tag="U4A13_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN1780340.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4160 a         2319 c         2880 g         3744 t
ORIGIN      
        1 atccattttt tattcatcaa aattactgaa aaaagaaagg aagaaataaa tttgaagaaa
       61 acaaattctt caggaaaacc gaaacgcgac ttcactaaat tatcgacacc ccatacgtat
      121 gtcattattt ttggtgtagt catctttgca tggatactga cttttgtcgt tccagcagga
      181 aagttcagca cgcaagatat tgaatacaaa gatgcaaatg gcgaaacaag tactcgtact
      241 gttttacgtc aagattcctt ccgttatgct tacgagctgg ataagtcgta tgtgtttgat
      301 caattagaag aactgcaaga tcatccagca gaacgtgaaa aactggatgt tccggaaaaa
      361 ggattggaaa aagtcattgc tgacggagaa aaaaatctta cgcaggaaaa actagatgaa
      421 atctccttga cagatgatgt gctttacgat cagtacggcg aaaatatcta tgatacttct
      481 aaaaaattgc ataaaacagc caaaatatgg ggaacagatg attttggcgg ctttggtttc
      541 ttaaattttg tttttgaagg actagtatct ggagataagt atggatctgc tgtagggatc
      601 gctgcattga tccttgttgt tggaggtgct tttggtatta ttatgcgtac cggagcgatc
      661 gacgcaggta tctatgcgtt tatcagtaaa actaagggac tggagcgttt agctttaccg
      721 ctattattct ttgctttttc attcggtggc gctacattcg ggatggcaga agaggtaatt
      781 ccattctcaa tggtgatggt tccatttgtc attgcgttag gatatgactc gatcgtagca
      841 gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga cctcttggat gagcccgttt
      901 agcgtagcgg tggctcaagg gattgctgga atccccgtgt tgtctggtgc gacattccga
      961 ttgattatgt gggtagttgt tactgcatta gccgctggtt atatgatgat atatgcagaa
     1021 aaaatccgta aaaaaccaga aagttcgttg acttacaaat cagatgatta tttccgttct
     1081 catatcaaaa agacatctga tgaaaataaa aaattcatgc ttgggcataa attgatctta
     1141 ttagagatgt tagttgtcct tgtctggatc gtttggggcg tgactcaaaa aggctattac
     1201 attccagaaa tcgcttctca attctttgtg atgggattag ttgctggaat cattgctgtt
     1261 cttttcaaat tagatggaat gaaagtcaat gacatcgctt cttctttcca atctggtgca
     1321 gctgatttag ctggtacagc aatcgtagta ggtatggcca aagggattct tcttgtcttg
     1381 ggaggatctg atgcatctgt tccttcagct ttgaatacga ttttgcatgg gatcgggacg
     1441 gcgttgaccg gtgttccagc tgtcattggt gcttgggcga tgtacatctt ccaaagtttg
     1501 ttcaacttgg ttgttacatc aaattcagga caagcagctt tgactatgcc aatcatggct
     1561 ccactggcag atttagtagg tgtatccaga caagtagcag ttcttgctta tcagcttggt
     1621 tcaggtttta tggacgcttt tacacccgta tcagccagct tgattggtgt gctaggtgtt
     1681 gcacggatcg aatgggcaaa atgggcaaga ttccaaatca agatgcaagg attcctgttt
     1741 gttttaggaa ctattttcat catgattgcc attgcaatag gtctgcagta gtactggaga
     1801 cctgtaaata aaaaagtaat ccaaacagta gataaggact ccaagaaccg tcattttacg
     1861 gtaaaatcaa aggagtaaag atcctatcgt ttggattatt ttttaagatc ctatcatcag
     1921 ttatacttaa agaaagagga aaaatgtatg aaaatcattc gacaaattga agtgtttgca
     1981 ccagattact taggaaaaat ggatgtactg attgccggtg ggaagatttt ggctgtagaa
     2041 gaacagctag aaggcggcta cgaaggagtg gaagtagaag aactttcagg tgagggaaaa
     2101 gtactaaccc cgggtttcat tgactgtcat ttccatattt taggtggagg aggagaaggc
     2161 ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat tgactacagc tggtgtaacg
     2221 acagttgtcg gttgtttggg aacagacgga gaaggtagag atatgactgc gttgatcagc
     2281 aaagcgaaag gtctggaagc agaaggaatc tccacttatg tatacgaagg ttcgtaccga
     2341 ttgccggtaa aaacagtaac agattctatc atcaaagact ttttgacaat tgataaaatt
     2401 atcggaatcg gagaaatcgc agtatccgat caccgttctt ctcagccaag ttttgaagag
     2461 ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt tatccggaaa agctgggatc
     2521 atcaatgtcc acttaggcgg aggaaaaaga aagctagagc tattgacacg aatcgtagaa
     2581 gaaacagaaa ttccgatcac acaatttttg cctactcatg caaatcgcac accagaatta
     2641 tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga ttgacttcac tgctagcgaa
     2701 gatcctgatt tttgggagaa gacagatggt gaagttcgct tcagcaaagc actgaaacga
     2761 ttgatcgagg aagatgtttc tttagataat ttcacgatga cctcagatgg acaaggaagc
     2821 ttgccttatt ttgatgaaaa caatcatttc ctagggttag gagtaggaag tgccaaggca
     2881 ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta tccctttgga aatcgcctta
     2941 cgagctataa ccagtaatcc agcacgtata ttgaaattgg ataaaaaagg aaaaatcgaa
     3001 attggtgctg atgcagatct gtgtatcttg gataaagaaa cgcttgatat tgatacagtt
     3061 atcgctaaag gagaaataat ggtccaagaa aaagaagtca aagtatgggg gactttcgaa
     3121 aaaagttttt aatcaacagc tctaactatc ttttttagaa aacgagcgtt ctgtttgtct
     3181 gtatctgttg ttgatgttac cctgaaggta gatcaataaa caaactaaga ggaggaagca
     3241 caatggcaga tttaaaagga cgttctaaag atatgaaaga taaagtagtc ggtgaaggaa
     3301 aagaagcata cggcaaagca acagacgaca agagcaaaga aacagaaggg aaagcacaat
     3361 cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg cgacgaagtc aaagacaagt
     3421 tttctcgaaa agacgaaaaa aaataaaaac cacttattac taattgaatc aagcaaataa
     3481 aaaatccgaa ccatatctgc gagacagata tggttcggat tttttatttg atcattatcc
     3541 tgttttttaa acttcgattt tttgaaattt tttatttttt agttcaaaga cttcatcaca
     3601 gctttttgct acttcaatct catgagtcac gatgataata cagcgatttt gttcatgggc
     3661 gatttcttgg aaaagtttga cgatatcttg tgaagttgtt tcatctagat ttccagtagg
     3721 ctcatccgct acgattagtt gatgttcaca gcagatagca cgaacgatag cgactcgctg
     3781 ctgctgacct cccgaaagtt tggtcacctg tttttttgct aatatttcat caattcctac
     3841 acgagccagt cgatctaaag caacttgtcg ataattctct tgcttggtct tagtgatttc
     3901 cattgctgct atgacgttgt ctagagccga taaataagga agcaagttgt aagcttggaa
     3961 aataatcgag acatcatttt tacgataact tcttaacccg attttgttca gtgaacgatc
     4021 ttgatagtat acctttcctt cttttggtgc atctagtcca gaaataagcg aaagaaaagt
     4081 cgtttttcca gaaccacttg ctcctaaaat agcgtacatt tttccagctt cgaaattcag
     4141 ttctacatct ttataaagcg catcttgcgg atcttgatac caaaatccca attgttctgt
     4201 ccgtaacatc aaattcctcc tttagtttaa taggattttt cttgggttta atcttaatat
     4261 ccctatagaa gctaaaaaga cagaaaacag actgattcca aatgctactc cggctaatag
     4321 tcccaattgt gcaggtttga cattgatttc caattcgctg acttgatcag aaacagcaaa
     4381 tggattattc gtggttcctt gttgaccatc gcgttgaccg ccttgttctt gtctgttacc
     4441 tggcatttgt ccaccgccag gagcttgctg gttttgtgtc gtggtttctg tctgttggtc
     4501 aagtagctgc tgtcccacgg cattcgcgac gacgtttccg ctaagcgaag caactcctaa
     4561 tgcaagaatc atacaaatag ccacttcggt aaacaattgg agaatgattt tacttctgga
     4621 ttctcctaaa gatagcaaga cgcctaactc atgccgacgc tcccgaatgg taatcataat
     4681 gatcagagta agtatgataa taccggctac agccactagt aaaacaacat ttttagaaaa
     4741 acttgccacg ttgtttaaag gttccaacat tgactgatac atggaatcat ttgattgtaa
     4801 gctaaatgta tctgtgtcga tcagcttctc agcttctttg agaaactcat ccattttatc
     4861 tggatcagat agagtgtaaa tagcagaatc tatcgtgtta tcgtcagatg tcccatttag
     4921 ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac ggattcatga agttgaattt
     4981 catacccata ctgccgcttg tttcgctgga ttcataaatt cctttgatcg tcatttcata
     5041 agtggtatct tcatcttcag tactcgtgat tgtgaaagta tctccgactg ccaggtcatt
     5101 tgtttctgct agagtagaat caatgactac attgtttgtg cctttgtcgc tgtcatctat
     5161 tccttcacca tccgtgattt ttgctgttcc atcagaaaaa ttactatttt gtgcagttgc
     5221 tgacacacca ctgatttgaa agtctgcttg agtcatttgc ggattcccgc ccattcctcc
     5281 agcaggttca ccgcctgcat cagtagtttc tgaactgtct tcacttgaat cagaactagt
     5341 gatggcagtt attccatctt ttgccatagc tgaggcggaa ctttcaaagg aataactctt
     5401 tacattatct aattcagcta ttttttccgc gtccgttaag gaaactggtg taagagaaaa
     5461 actgcctgga tctggacgtt cttcagtggt agaaccgctt gaagaagttt gttcctgttt
     5521 tttaaatgaa gcttctcgat tggcacttaa agtcactgtt gctccaactt ctttttgtgc
     5581 ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg ccagctagca caaaaataag
     5641 aatggctgtg aacacggcaa acaataacaa tgaacgtccc cattttactt ttgtactttt
     5701 ccatgcacgt ttcataaaat tcatattttg catccctcct agtagatgca tttgataata
     5761 gacaacctat cttaaagcta gcttaaatct tatgctgttt gcacaccatc aacatgaggt
     5821 tctgtatata taaaaagtca ctatagttat tgcaaaaaat gtggtaaaat gaaaaaagta
     5881 tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca aaagaaatcc tagatgctac
     5941 agagggattt ttacgtgcat agttgttcta acaattgctg atactagcga tttagtttat
     6001 ttgttgtata ataatgggga acagctgaat acttatataa gtattctggc tatagcgttt
     6061 cgcaaaagat ccattttgtt aatgaagtat atccaaaata aaatgcccat ccaactgtca
     6121 gaacgcggat gtgctggtaa ttttcctgct gattctccat aagttgagaa atagtgattt
     6181 tgcatatgct tcattggaat ggctcatata tttagataaa gaagaaaatc gacatcttta
     6241 aacttggatg tcataagaag aggtgaagtt tgtgagtacc attaaaatta tcccattagg
     6301 cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa gtgggcgagg atatttttat
     6361 tttagattgt gggctaaaat atcctgaaaa tgagttgttg gggatcgacg tcgtaatccc
     6421 tgattttacg tatttagaag aaaacattga tcgtgtagct ggtgtctttt taacacatgg
     6481 acatgcagat gcgatcgggg cacttcctta cttgttgtca aaaatttctg taccagtatt
     6541 cgggacagag ttgacgatcg agttagcaaa attgaatgtc agtcgtaatg aagcagccaa
     6601 aggatttaag gatttccatg tggtagatga gcatacagaa atagactttg gtgaaacagt
     6661 agtgagcttt ttccgtacca ctcatacgat accagattca gtggggatca atctgaaaac
     6721 aaaagaaggt agtatcgttt atacgggaga ttttaaattt gaccaaacgg ctatcccaat
     6781 gtatcaaaca gattatgcac gattggcgga aatcggcaaa gaaggagtct tagccttact
     6841 aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct tctgaagccc aaatagcaga
     6901 tgaagtattt gatacgatca aatattggga agggcgtatc atcgtcgctt gtgtagcaag
     6961 taatttgcaa cgagtgcaac aagtattgga tgcagcagaa aaagcacaac gaaaagttgt
     7021 cttgactggt caagatttcg aacgaattat tcgcacagcg atgcgtttag aaaaacttca
     7081 attaccaagt gaagatctgc ttgttacgtt gaaagacatg aaaaactatg atccagaaga
     7141 actgatcatt ttagaaactg gccgaatggg tgaacccatc aagtctctgc aaaaaatggc
     7201 aaacggtaca caccgtaatg tcaagattga agaaggagat ttggtttata tcactactac
     7261 gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa gatatcattt atcgagctgg
     7321 tggaacagtg aagttgatct ctgagaatat gcgcgtttct ggccatgcca atcctaatga
     7381 cttgcaatta atgatcaatt tgatgaaacc aacatatttt gtgccagttc aaggagagta
     7441 tcgtgaatta gctgctcatg cagatttggc tcatgcagta gggatgccat acaaaaatat
     7501 ctatattact ggacgcggag acgtgctgga atataagaac aagcgtatga gtgttgcagg
     7561 aactacaagt gcagaaaatg taatgattga tggattaggc ataggtgata tcggaaacat
     7621 tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt ttcgtagcgg ttgtcactat
     7681 cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt acttcacggg gattcgttta
     7741 cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat atgattgaaa caatcgttga
     7801 gaaacattta gaaagcaatg attttgagtg gagcaaatta aagcaagaca tccgagatca
     7861 attaggccgt tacttatttg aacaaacaaa acgccgccca gtgatcttgc cagtcattat
     7921 ggaagctact caacgtcgaa ataaaaaata aacaaaaaga gtaagatgaa cctctctata
     7981 gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat ttttgaatac agcatccctc
     8041 gctttataat aaacgataga ataaagggag tgaatcaatg atgagtcaaa caaaagtcga
     8101 acgttattta gaagaacaaa agatcagcta tcaaccactt gattttagtc atgtggtaga
     8161 agaatctttc acagaagaac tacaaaaaag agggattgat ccagctttga tatgtaaaac
     8221 tctcgtagtg aaaggaaaca aaactggggt gatcatcgca ttgattcctt tgaacgatca
     8281 cttagattac aaaaaaacga gacaagtgac gaaagatcgt aaagttggtt ttcctggaat
     8341 ggattttgta ttaatgcata caggatatcc ccacggagca aacaccccta ttggaataaa
     8401 actcgcacat cctgattatt gttttttagc agacagttct ttaaaagaaa aagaagaagt
     8461 aattgtgtca agcggtgaaa tcgggagaag ccttcggata aaagtagccg atttagaaaa
     8521 gatcgtccat cctatttacg ctgatttaat tagttaaggg acgagaagag aagctaagca
     8581 gagcagagac ctcaaacttg agattatatc tgcttggtac ttggtgcatc ctgtattatc
     8641 tgttacaatt gctttgtaca tactgtacta tcaatccaat gaaaatatat ttagcagcta
     8701 tattttttca ttgatgtgca agtgatgaaa tcgtttttaa taaaaaagat acagagaaaa
     8761 cgcgaggtgt aaaatatgaa aattacgtat catggacatt cctgtttaac aattgaaatg
     8821 gaaaatggcc aacgcgtgat gattgacccg tttattacag gaaatccatt gacagactta
     8881 actgcagaag aagtaaagac agattggttg ctgattactc atggtcatag tgaccacata
     8941 ggagatatga ttcctattgc aaaagaaaat caagccacaa ttatcagtat tgttgagatt
     9001 gctgattatg cgaacagcag aggagtagat tcattcggta tgaatatcgg tggaaaacat
     9061 gcgtttccat tcggcgtggt aaaattcgta catgcacagc atagttcaag ctatgaagta
     9121 gacggtatcg ttcagtatat gggcgagcct agcggaatta ttattcaagc agaaggaaaa
     9181 acgatttatc atgcagggga tactgcttat ttcagtgatc taggattgct tgcagaagaa
     9241 ttcgatattg atgtcgcttt cttgccgatt ggcgataact atacgatggg gccagaagat
     9301 gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag ttccaatcca ttacaatact
     9361 ttccctgtca ttgaacaaga tcccgaatct tttgtagagt tactggaaga tagtgaagga
     9421 aaaataatga gcgtctcgga aacgctggaa gtgtaggcag aaaatggcga caaaacatga
     9481 tttgatttta gaatatattg aaagtttacc tgttggcaat cgaatttctg taagaagcat
     9541 cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt gcaatcaaag atgcagaaaa
     9601 tgtcggacta gtttcaacaa ttcaacgagt agggaccatc cgtatcgaac gcaaattaaa
     9661 aaaacatatt gaacgtttga catttggcga ggtcgtcagg atcattgaag gggacgtttt
     9721 aggcggggcg gcaggattag acaaggtctt gaataaattc gtcatcggcg cgatgactaa
     9781 acatgccatg gaacgctata ttacgccagg atcactaatg atcgtcggca accgggcgga
     9841 agtccaaaaa ttagcattgg aagatggcgc cgcagtattg atcacaggag gattcaatac
     9901 gactgctgag attgcaaagc tagctgatga tcttgaaatg ccgatcttac gcacctctta
     9961 tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta agcgaccaat tgatcaaaaa
    10021 agatatcatg cttgtcagtg atatctatat gccagctgaa aagactcatt atttgcagca
    10081 gacagatacg atttctgatt atcagcgttt gtcagaagaa acgcagcatt cgcgttttcc
    10141 agtcgtcaac cgccatcatc gtttgatggg gattgttacg gcaaaagatg ttttagggaa
    10201 atcgccaaac cagatcatcg atcgggtgat gacgaaagaa ccaatcagtg taaagaaaac
    10261 aatgagtatt gcctctgtta gccatcaaat gatctgggat gggctagaag tcatgccggt
    10321 cgtttccgat gacttgacat tggaaggctt gattacccgg caagatgtaa tgaaagcgat
    10381 gcagcttgtc cagcgacaac cgcagattgc agatacgatt tcggatcaga tatctggaaa
    10441 tatccatacg attgatactg accgtgaagg aaatcgtttg gaaaacccta agttcaaatt
    10501 cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt tcttttggcg tacttagcga
    10561 aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac caaaaacgaa atatcttgat
    10621 tgaacaagtg aacctacatt atttgcggct gatccaattg gaaagcgaat tagacattcg
    10681 tccacgtgta ttggaaatcg gcagacgttc cgctaaactg gatatcgaag tgtttattga
    10741 aaacaccatc gtttccaaag caatcgttgt ttgtcaagta atggaacgtt cttagaaagc
    10801 aggagtgaaa tgacagtaca gcaagaaata gtagatacaa taaaaaaata cgataaaatc
    10861 attatccaca ggcatttgcg ccctgatcct gatgcattag gatcacaagt aggattagcc
    10921 gaactattac gggaaagttt cccagaaaaa gaaatacgtc aagtaggtga aacgattgaa
    10981 gggcttcgct ttttagcaga aatgcaagaa gttgaagatg tcttctacca aggagcgcta
    11041 gtcatcgtga ctgatacagc taattcaccc agaatcagtg atcaaagata tcagttggca
    11101 gaaaaactga tcaaaatcga ccatcatccc aatgatgacc cttatggaga tcttgtgtgg
    11161 gtaaatacaa aagcaagtag ctgtagtgag atgattgcag aatttgcttt gatgtttcct
    11221 gaatctttaa agatgaacgt taaggctgca cggttacttt atgcaggaat cgtcggagat
    11281 acagggcgct ttttgtatcc ctccacgacg tcccgtacat tagagattgc ttcgatattg
    11341 agaaattatc catttgatgc aagtgctttg aacagagaga ttgaacaaat gccgatgaaa
    11401 gtcgctaaac tttccggtta tctttatcaa aatatccagg tggatgaaaa tggtgcagga
    11461 agaataatcc ttccgcaaga aattcttgat aaatatggaa tcgatgattc agagacagct
    11521 tcgatcgttt cacttcctgg agtgattgac gaagtgctgg cttgggggat ttttgttcag
    11581 cagccttcag ggtattaccg cgtacgttta cgttctaagg ggccgatcat caatgaattg
    11641 gctaaacgcc atcatggagg aggccatcca ttagcaagtg gtgcgaatgc aaaagattta
    11701 gcagaagtag aggaaatcta taaagagatc caagatatat gccaatcgta taagagcaga
    11761 aaaacagaat gatacttcgt tcgaaaacgt gccttttgta gtaaaaatca caaatgtatc
    11821 ttttcaagat cttctttttc cgttacaata gtgctcacta aagattttaa aggagaaaga
    11881 agatgacatt accaaattgc ccagaatgtg gctcagagta cacatatgaa gatcgaggct
    11941 tgttgatttg cccagaatgc gggaacgaat ggaacaaaga agaacaagaa actgtcatag
    12001 aagacggatt agtggtatta gatgcaaatg gaaaccgtct gttagagggc gatagcgtga
    12061 cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc cattaaacaa ggaacaaaag
    12121 tcaagaacat ccgtttagta gaaggcgatc ataatatcga ttgcaaggta gaaggatttg
    12181 gaccgatgaa actgaaatcg gaatttgtga aaaagaatta aagaatattc aaggtctttt
    12241 cacattcttt tcctactact aatgataaaa taaagtaaac gaaaaggagt gaaacgaaaa
    12301 tgtatggtta tggaatgtat ggttttttag atccgacatt tcttttggtc attcttggat
    12361 tagttatttc cggaattgcg tcagcgtacg tcaatagtac tttccgaaaa tacgatcaag
    12421 tgagaagcaa aaacaatgtg actggtacac aagcagcaca atatatatta caaagtcaac
    12481 agataaataa tgtcggtgtt caacaaattg ccggagatct cacagataac tacaattcag
    12541 gtaataagat gttgagtttg tctcaagcaa ctgcacagtc tacttcagtt gctgcgatag
    12601 gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc ttcgggttat gtcccattga
    12661 agattcgtgc agcacttgtt ccagcagcta actttggttc gatgatttcg ttccccttga
    12721 ttatggtagg tgtattgctt agctggaact ctacgttaat caatatcggg attttcgctt
    12781 tttctttagc attacttttc caattggtga cattaccagt cgaatttaac gcgtctcggc
    12841 gagcaatcca aattttgagt gaaggcggac tactgacgga agaagaagta ccgatggcaa
    12901 gacacgtatt atttgcagca gcactgactt atgttgcagc agcgctttcg acattcctgc
    12961 agctgttgcg cttgatctta ttgttcggcg gcaaccgaag agattaaaat aaagaagttg
    13021 tgaaatcagc gctttgatct gagctaatac cgagagttag ctgattgaat accatttatt
    13081 tggaataagg gctctttgtc aat
//
LOCUS       DASJPC010000073        13032 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJPC010000073 DASJPC010000000
VERSION     DASJPC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.073"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..914)
                     /locus_tag="U4A13_002067"
     CDS             complement(<1..914)
                     /locus_tag="U4A13_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN1780341.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U4A13_002068"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U4A13_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN1780342.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="U4A13_002069"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="U4A13_002069"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN1780343.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="U4A13_002070"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="U4A13_002070"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN1780344.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="U4A13_002071"
     CDS             4440..5321
                     /locus_tag="U4A13_002071"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN1780345.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="U4A13_002072"
     CDS             5318..6034
                     /locus_tag="U4A13_002072"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN1780346.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="U4A13_002073"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="U4A13_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN1780347.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="U4A13_002074"
     CDS             6751..7752
                     /locus_tag="U4A13_002074"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN1780348.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="U4A13_002075"
     CDS             7749..9110
                     /locus_tag="U4A13_002075"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN1780349.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="U4A13_002076"
     CDS             9070..10221
                     /locus_tag="U4A13_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN1780350.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="U4A13_002077"
     CDS             10196..11365
                     /locus_tag="U4A13_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1780351.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="U4A13_002078"
     CDS             11540..12919
                     /locus_tag="U4A13_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1780352.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
BASE COUNT         4340 a         2275 c         2842 g         3575 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gc
//
LOCUS       DASJPC010000074        12899 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJPC010000074 DASJPC010000000
VERSION     DASJPC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.074"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="U4A13_002079"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="U4A13_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN1780353.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="U4A13_002080"
     CDS             complement(1195..3036)
                     /locus_tag="U4A13_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN1780354.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="U4A13_002081"
     CDS             complement(3218..5533)
                     /locus_tag="U4A13_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1780355.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="U4A13_002082"
     CDS             complement(5718..6578)
                     /locus_tag="U4A13_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN1780356.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="U4A13_002083"
     CDS             complement(6637..7158)
                     /locus_tag="U4A13_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN1780357.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="U4A13_002084"
     CDS             7436..8197
                     /locus_tag="U4A13_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1780358.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="U4A13_002085"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="U4A13_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN1780359.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="U4A13_002086"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="U4A13_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN1780360.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..>12899
                     /locus_tag="U4A13_002087"
     CDS             12557..>12899
                     /locus_tag="U4A13_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1780361.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAE"
BASE COUNT         3693 a         2706 c         2294 g         4206 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccgacca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaa
//
LOCUS       DASJPC010000075        12734 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJPC010000075 DASJPC010000000
VERSION     DASJPC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.075"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U4A13_002088"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U4A13_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1780362.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="U4A13_002089"
     CDS             complement(322..891)
                     /locus_tag="U4A13_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN1780363.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="U4A13_002090"
     CDS             1077..1979
                     /locus_tag="U4A13_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN1780364.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U4A13_002091"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U4A13_002091"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN1780365.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U4A13_002092"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U4A13_002092"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN1780366.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U4A13_002093"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U4A13_002093"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN1780367.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="U4A13_002094"
     CDS             8547..8732
                     /locus_tag="U4A13_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN1780368.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="U4A13_002095"
     CDS             complement(8794..9990)
                     /locus_tag="U4A13_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN1780369.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="U4A13_002096"
     CDS             complement(10160..11596)
                     /locus_tag="U4A13_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN1780370.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="U4A13_002097"
     CDS             11889..12698
                     /locus_tag="U4A13_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1780371.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DASJPC010000076        12670 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJPC010000076 DASJPC010000000
VERSION     DASJPC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.076"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4A13_002098"
     CDS             6..305
                     /locus_tag="U4A13_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1780372.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U4A13_002099"
     CDS             378..1739
                     /locus_tag="U4A13_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN1780373.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U4A13_002100"
     CDS             1761..2021
                     /locus_tag="U4A13_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780374.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U4A13_002101"
     CDS             2035..2787
                     /locus_tag="U4A13_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN1780375.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U4A13_002102"
     CDS             complement(2881..3486)
                     /locus_tag="U4A13_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1780376.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U4A13_002103"
     CDS             complement(3539..4513)
                     /locus_tag="U4A13_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN1780377.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U4A13_002104"
     CDS             complement(4891..5610)
                     /locus_tag="U4A13_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1780378.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4A13_002105"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4A13_002105"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN1780379.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U4A13_002106"
     CDS             7161..8558
                     /locus_tag="U4A13_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1780380.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U4A13_002107"
     CDS             8639..9079
                     /locus_tag="U4A13_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1780381.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U4A13_002108"
     CDS             9227..9553
                     /locus_tag="U4A13_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1780382.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U4A13_002109"
     CDS             9554..10303
                     /locus_tag="U4A13_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780383.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U4A13_002110"
     CDS             complement(10383..10838)
                     /locus_tag="U4A13_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1780384.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="U4A13_002111"
     CDS             complement(11265..11540)
                     /locus_tag="U4A13_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780385.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="U4A13_002112"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="U4A13_002112"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN1780386.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2174 c         2587 g         3854 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DASJPC010000077        12280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJPC010000077 DASJPC010000000
VERSION     DASJPC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.077"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="U4A13_002113"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="U4A13_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN1780387.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="U4A13_002114"
     CDS             complement(642..1364)
                     /locus_tag="U4A13_002114"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN1780388.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="U4A13_002115"
     CDS             complement(1500..2381)
                     /locus_tag="U4A13_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN1780389.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="U4A13_002116"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="U4A13_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN1780390.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="U4A13_002117"
     CDS             3621..4100
                     /locus_tag="U4A13_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN1780391.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="U4A13_002118"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="U4A13_002118"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEN1780392.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="U4A13_002119"
     CDS             5401..5529
                     /locus_tag="U4A13_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1780393.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="U4A13_002120"
     CDS             5533..6078
                     /locus_tag="U4A13_002120"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN1780394.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /locus_tag="U4A13_002121"
     CDS             complement(6532..8223)
                     /locus_tag="U4A13_002121"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN1780395.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="U4A13_002122"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="U4A13_002122"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN1780396.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="U4A13_002123"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="U4A13_002123"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN1780397.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="U4A13_002124"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="U4A13_002124"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN1780398.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3378 a         2533 c         2206 g         4163 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttattc attttttatt ctgtatacac tttcaaaaaa atatttattc
    12241 attgaaaaaa tttttttgaa taaattaaaa aaataaatta
//
LOCUS       DASJPC010000078        11859 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.078, whole genome shotgun sequence.
ACCESSION   DASJPC010000078 DASJPC010000000
VERSION     DASJPC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.078"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..586)
                     /locus_tag="U4A13_002125"
     CDS             complement(5..586)
                     /locus_tag="U4A13_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1780399.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="U4A13_002126"
     CDS             complement(769..1395)
                     /locus_tag="U4A13_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1780400.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="U4A13_002127"
     CDS             complement(1417..2748)
                     /locus_tag="U4A13_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN1780401.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="U4A13_002128"
     CDS             complement(2903..3304)
                     /locus_tag="U4A13_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN1780402.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="U4A13_002129"
     CDS             complement(3390..3596)
                     /locus_tag="U4A13_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780403.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="U4A13_002130"
     CDS             complement(3691..5193)
                     /locus_tag="U4A13_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN1780404.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="U4A13_002131"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="U4A13_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN1780405.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="U4A13_002132"
     CDS             complement(5764..6381)
                     /locus_tag="U4A13_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN1780406.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="U4A13_002133"
     CDS             6602..7975
                     /locus_tag="U4A13_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1780407.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="U4A13_002134"
     CDS             8050..9162
                     /locus_tag="U4A13_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN1780408.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="U4A13_002135"
     CDS             complement(9234..10871)
                     /locus_tag="U4A13_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN1780409.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="U4A13_002136"
     CDS             complement(11039..11647)
                     /locus_tag="U4A13_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1780410.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3135 a         2482 c         2140 g         4102 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actagttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tggtttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gactctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggtctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tgtcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttattt
//
LOCUS       DASJPC010000079        11775 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJPC010000079 DASJPC010000000
VERSION     DASJPC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.079"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(182..1798)
                     /locus_tag="U4A13_002137"
     CDS             complement(182..1798)
                     /locus_tag="U4A13_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1780411.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            1916..2134
                     /locus_tag="U4A13_002138"
     CDS             1916..2134
                     /locus_tag="U4A13_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780412.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            2329..2790
                     /locus_tag="U4A13_002139"
     CDS             2329..2790
                     /locus_tag="U4A13_002139"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1780413.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2983..3222)
                     /locus_tag="U4A13_002140"
     CDS             complement(2983..3222)
                     /locus_tag="U4A13_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780414.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3246..4769)
                     /locus_tag="U4A13_002141"
     CDS             complement(3246..4769)
                     /locus_tag="U4A13_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN1780415.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(4787..4909)
                     /locus_tag="U4A13_002142"
     CDS             complement(4787..4909)
                     /locus_tag="U4A13_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN1780416.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(4939..5922)
                     /locus_tag="U4A13_002143"
     CDS             complement(4939..5922)
                     /locus_tag="U4A13_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1780417.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5946..6095)
                     /gene="rpmG"
                     /locus_tag="U4A13_002144"
     CDS             complement(5946..6095)
                     /gene="rpmG"
                     /locus_tag="U4A13_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN1780418.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6116..6493)
                     /locus_tag="U4A13_002145"
     CDS             complement(6116..6493)
                     /locus_tag="U4A13_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780419.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6525..6704)
                     /gene="rpmF"
                     /locus_tag="U4A13_002146"
     CDS             complement(6525..6704)
                     /gene="rpmF"
                     /locus_tag="U4A13_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN1780420.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6719..6988)
                     /gene="rpsN"
                     /locus_tag="U4A13_002147"
     CDS             complement(6719..6988)
                     /gene="rpsN"
                     /locus_tag="U4A13_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN1780421.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7511..9277)
                     /locus_tag="U4A13_002148"
     CDS             complement(7511..9277)
                     /locus_tag="U4A13_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1780422.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9237..10990)
                     /locus_tag="U4A13_002149"
                     /pseudo
     CDS             complement(9237..10990)
                     /locus_tag="U4A13_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11100..11669)
                     /locus_tag="U4A13_002150"
     CDS             complement(11100..11669)
                     /locus_tag="U4A13_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN1780423.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3709 a         2281 c         1698 g         4087 t
ORIGIN      
        1 ccaaaataag taatctaacc gactgttaac taaaccattg caggtcccgt taaagatact
       61 tgtgcctgtt gtcaccgctt ctttttcttg tccacaagcg cttaacacac ttaataaagc
      121 ccctgctatt attaataata atatcttttt cttattaaaa acctcctcat ctatttccag
      181 attagtaaat gtagtaatct gtgctttctt ttttctcaat actaataact aacttatggg
      241 gtaaacaaat actcgtttga ccgggttttg agatccatcc agttctaacc gcgatttgat
      301 caggactatt atcttcttta actcgaattc ttccatcttt aatctcaaca atattatatt
      361 gtcccttcgc aggatgataa ttcacgagtt tatgattgat cttatccaaa tcgaagcgat
      421 ccatttcctt gccatcaatt cgaacgatgg catatttgat agcatcttca gttgtttcat
      481 tctccttttc tatagagaat acaaataagg gaataaaaga ggtaataatc aaaatgccaa
      541 taattatgac atcgcctatc ttgacaccag cttttcttaa aaatttcatt aacgatatct
      601 ggctaaacat attagacttt ttccatggaa atggtgaaat ttttcggaaa ttatctgcat
      661 taagagaatc agttaaacaa ccctcaagtt gttccttaat acattgttga tccatcttca
      721 taccaataaa aactagttta ttaatgcgtt caccaaacaa cggatgccag gcttccttca
      781 attcgggatt tttttgtata atcatttgac actcaatttt aggtaaacta gctacccaat
      841 aatttaatga ttcaatactt ttatagctac ccgatgtttc aaagatcgtc gaaaaatcac
      901 tatgttgggc aaaccaaaga aacccttttg ctcttgtaat attgccagga aaatttttat
      961 ccgtaagatg ggcaaatttt tgtgcgtcaa ggagtaaccg atttcgatag ataaaacttg
     1021 agataccata ttcttctgtt tcaggaatat gattttgata gccaagctct aactctttga
     1081 accagcccac tgaataggaa gtctcatcta tattaaaaag atttgtattt agaattttcg
     1141 ataagtctac ttcaccttta attgtcctaa ttattttggc attggactgt aacttagcaa
     1201 ttagaccgtt aattttgtaa atcgtcttgg ttgacactaa gtcactatta tttaaaagta
     1261 atatgttaca acactcgaat tgatcaatta acaagccacg aatatcctgt tcttcttcaa
     1321 ctacattacc ttcagctttt ctttcctgaa tcttttcacc cgtttcgaaa ttatcccaaa
     1381 aacgttttac atccactact ataataatgg caccaatctc taatcgttgg gtcaaatcaa
     1441 tgtctaaagg gtttttagct aatataattg cttgaataat tggaattggt ttgctgattc
     1501 ctgaagcttc aataacaatt tgattcaagt cagaaaattg cactaattga tctagttcaa
     1561 caatcaaatc ttcgcgtaat gtacaacaaa tgcatccatt cggcatcgaa acaagttttt
     1621 ctccagttct tttaaaacct ttcttgtcga ctaatacccc atcaacattt acttccccta
     1681 tgtcatttac aatgacagca atttcctttc cgtgatcacc gtacagaata tgatttagaa
     1741 gtatcgtttt tcctgaacca agatagccag ttaagattgt aataggtatt tttttcatag
     1801 ggaacctctt tctatacctt gaatttacac tttaagtgca acacataaaa atgagtcata
     1861 atcgatatcc aactagtata agtttgtcga gaacactcct agaaggaaaa taattatgag
     1921 ccagtatcgt catcttacta tactcgaacg agaaagaatc tttctgcttc acgaagaagg
     1981 attttctatt agtataattg gcaacgacat tcagcatagc ccgtttacta ttttacatga
     2041 actgagaaga aacaagaaaa aaactattca ccttccaaag cggaaaaaaa ttatgcttat
     2101 cgaagaaaaa aatgcggacg ttatcacatt ctaaatgatc cgtgagtttg gtcaattgtt
     2161 cgtcatttat ttgtcgattt tcagtggtcg ccagaggaaa tctcgaatcg tttgaagcat
     2221 gaacatatgg acttttcaat tagttttatc acgatctaac gtgcaatttg caatgatctt
     2281 ttcgatacag aacccctctc acacggcaat aaaggcctga tccgttcatt gcaccatcga
     2341 ggaaaaacaa gacatacaaa aaatcatgtc gagtctcgtg gaaagatttg ccttactcat
     2401 tcgatccatg gccgtccaaa atccgcagat gatagaagtg attttggtca ttggaaagcc
     2461 gatattgtag ctggcaaaac tggtggtact tgtttagtga ctttgacaga cagaaaatca
     2521 tgcttccttt tagcaggaaa gaaggtcccc agaaagaaag ccgcttatgt gagcgagaaa
     2581 atgattgaac ttttaagcag tattccaaat gaggcggtca aaacgatcac tccagataga
     2641 ggaaaagaat tctccaatca tttcgcagtt acagaagcat tgaataatat gcccttttat
     2701 tttccagatc ctcatgccct tggcaacaat gaacaaatta aaatacgaat agactcattc
     2761 gagaatactt gccaaaatcg aaagagatag ctacggtaga taactcaacg ataaatcaga
     2821 tgattgaaaa actgaataac agacctagaa agtgtttaga ttggagaaca ccttatgaag
     2881 tcttctttga taaagtgttg cgcttgattt gacaattcaa gacataataa aaagcagatc
     2941 aaatccaaat aagggaaaaa tgatctactc tggtattatc aattaattgg ccaagctatt
     3001 atgccgtttt gctaaacgtc tacttcttcc agtattgacc ttgataaaac aacaatcagg
     3061 atcccgtttt aatactctat gaactgtgtc aatactaaca tgtaaagttg cagcaatatc
     3121 agctaatgta ctcccattta atctcatttg ctttgcaaat cgataacgcc gataagcatc
     3181 aatattcttg gaatccatag acaataatac ttttcttacc atgtatttcc tccaaaaatt
     3241 gaattttaat gagccatcat ttcttgagca atttcatgtt tagacaatga agctggatag
     3301 aaagtaggcc aattatgcaa ttcattatag agtttttctt gactttctcc accaaagaaa
     3361 atatggaaat gctctgcctt acttggtgca attccatgat cactaaattg aatatattta
     3421 aaacgaccag cacctgcatc atcagtttca aaattaaaac gaacaccacg gtttcctttt
     3481 tcataattca aaattttgta tccaacatat ttgtatgtgt attgatgatg ttcaccgttg
     3541 actacaaaat caatggtgtt gtctttaatg ttgatagtat caatatctgt cttatagcca
     3601 gtagtataat aatctttgta ctcggcagct gtcatgtctt tattgatttt tgctttgtaa
     3661 tcgaaaactt catccaaaat tccatctttt aacaacggat aaacagattg ccattcgcct
     3721 gtataatcac tcaattcgcg atccttcaca tcggcatctt taaagtaccc attagcaact
     3781 gtcttttcat ccttcgcttc tttttctgga actacctctt caccagcaac catagttgtt
     3841 ttttctaacg cttttagatt gtcttccatt actgaaacat aatcttcacc attatccatt
     3901 tgttcttttg taagactttc aagaggattc agtacttcca attttatccc agcttcgttg
     3961 gccaaagttt ttgcaatttt atcattagca ttcttttcaa agtaaatata attgatctta
     4021 ttttttttaa catactcttt taattcagct aatcgaccag aagatggctc ttcttcagga
     4081 cttagtccag caataggaac ttgtttcaat ccataatcca aagctaaata tccaaaggct
     4141 gcgtgttgcg tcacaaaact tttttgtttc gcttctttaa gactagtggt atagtcagca
     4201 tccaaccgtt tcagtttagt caaatacttt tcagcatttg tctcaaatac ttttgctttt
     4261 ttcggataca atttaactaa ctgatccttg atatttgaaa cttccttaat tgccattttg
     4321 ggtgaaaccc acgtatgagg atctagttca tgatggtgat cttcttctcc gtgatcatga
     4381 tcttcttctt cactacctgg caataaaatc atgtcttttg ttccttctac tacattaggc
     4441 ccagctttct tccaactttc tttagctttt ggtacccatg attccatgtt ttcgttatga
     4501 tatacaaaaa catcagcatc atgagctttt gccatatcct tagctgaggg ctcatagtca
     4561 tgaggttctg atcccgctgg aaccattaaa tccacgttcg cttcatcgcc gacgatgttt
     4621 ttggtgaaat catacattgg atagaatgtt gttacaattt ctaattttcc atctttatca
     4681 ttcgatactt ttgcatcatt agtgctggag caagctccca ataatcctag agctgctatc
     4741 ccaataagtc ctacactgat tttcttcatt ctttttcctt ccttaattat gctaatttct
     4801 tcattttatt tcgtttatgt tctttgataa tttttaatgc cctttttcta gttttaatat
     4861 tcgggctttt aagtcgtcga tatgcactag atatatccgt tttctccatt agaaattacc
     4921 cccaatcact acttatcatt atttgctttc cgccagtaat ttatcaaaac tctgtttgat
     4981 cctttcttca tccaacattt ttccgattag aataatagtg gtttggtctt tttgtcgagt
     5041 ggtgtattga aaattaacct gttcgtttac ctcttgcatg gcaactataa aatcctgctc
     5101 ttgtaaagca atcaatcctt taaaacgata caggctccct tggttggtta aaatcagcca
     5161 gtcaatccat tggagtaaaa aatctttttt tatgtcttga tctgaagtta agagaattga
     5221 tttaaactca tgtacttgat ggtgttgatg atagtatttg ccattatagt gattttctga
     5281 tgaatcactt tcaaaaacta agggatgatt aaattttttc aaattataaa aatccttttt
     5341 ttgaatcgtt tcactatgtg tgaacattcg aatatcggat aaagggttaa tacttttgat
     5401 ttctctctgt aattttttta gtgcagataa attcaagttt tgttttctag acacaaaaat
     5461 gcgatctgcc atgacaagtt gcttaagggc ttgtggttct ctttcaataa gttcaaaact
     5521 gtcactatca ataacagtca ataaactatc gatatatact cttcctctta gttgaggcgc
     5581 tgttaaaagt gtttgaatga ttggttgggg atctgctata cctgtagttt caatgatgat
     5641 ttgtgaaatt ggttgttgat gttcttctat tgtttccagt aaagaaatca atattgctaa
     5701 taaatcactg cgaagactgc aacacataca tccattattt agttgtaaca cccgttcctc
     5761 gctatgaact aataatttgt gatcaatccc aacttcacca aactcatttt caattataat
     5821 aatttcttcc ctggggtagg gtgagacttg caaggcttga ttgattagcg ttgtttttcc
     5881 agcccctaaa aacccagaaa cgattgtaac tggtatgccc attttgtcac ctttttacca
     5941 cttagctatt tcacttcttt aaaaattgcc cgtctgcgca attttggcga atactttttt
     6001 aattctaacc gttcagggtt attccgttta tttttgctgg tgagatacag acgttctcca
     6061 gtttcgacac attccaaaat aatattttga cgcataaatc ttcctccttc aatacttatc
     6121 gatcttcaag atatgcttga atttgttgtg catcttcaaa agttaactcg ccagattcca
     6181 attgtgaaac taaattttct ttcatatcaa caggtatctc ccaataacca tcgatacatt
     6241 gaaataactc ctcgtcatac tcttcaaaaa ttggatagat tcttaaaaaa tcatcaaatg
     6301 ataagccttg gtcctcatag taatctttga ctaatccatt ttcaattgcc tgttgggtcg
     6361 gtaaacccgt ttcagtaata attcctctct ctaccatcga acgataaaaa gtgcatttat
     6421 ctagcgattg ctgggtcgtt tcaagataat actttaatcc ttcttcaact aaaggtgggt
     6481 aaataatttc cattttgatt ccctcctaaa agttatatat taaactattt tgaagctcct
     6541 ttaattactt tctttccatt atagtaccct tttaaagata catgatggct acgtctataa
     6601 tcaccattcg cttcgtcaaa tgaaatttct ggttttacta cagtttgatg agttcttctt
     6661 aaccgtttct tcgccttaga tgtttttctt gctggtactg ccatgatatt tcctcctttt
     6721 accaacttgc tttttttact ccaggaatca acccttgatg ggcaagttct ctaaacttaa
     6781 tacgggacat accgaacttt cgcatatacc ctcttggacg accatccgtc tgatcacgaa
     6841 gcctcagacg attcggattt gaatctcttg gcaatttagc gagagcggtg cgatctcctg
     6901 cggcttttaa ttcctgacgt ttatctgcat acttttcaat catcgccatt tgtttctttg
     6961 ctttagcaat tttcgacttt tttgccatat acctttatac tccttttatt ttgatttaga
     7021 cacctcaaat cgtaatcgtt acgttttgac actgatactt taccaacaag agttgctctt
     7081 gtcaacaata aataacatga aataaaactc atatgaatag ataactattt gaaaacgaat
     7141 ttatcggtac atagagataa tcaaatcatt taaaagaata tgcagtttag gctctgaaaa
     7201 ataagaattc acttttatta gtccaatcaa acacaaatac cgttttaagt agcttttcct
     7261 ctacttaaaa cggcaatatg aaagaaacta taacttttta ttcaatcttt tatgtttaac
     7321 tggtttatcg ccaatttttt aaaactttga ttattcctta tattataaga atagacatta
     7381 agtgaactaa ttcaatcata ctgattcttt cgtgtattca aaattagatg ctgtttaata
     7441 aaattattct ttttgaaaaa gaattataac tatatcccaa aaattgtagg ttaatttaaa
     7501 gcctatttca tcatcgtgat tgagcttctt tttcatagac gaatttctca gatagtttag
     7561 ttttctttat gagatttttg tatatttctg accttcgata caactcatta tgagtacctt
     7621 gatcaactag ttttccttga tccatcacta caattttatt taccttttca atagacttca
     7681 agcgatgcga tataattagt actgtcttat cttgtattaa acgatttaaa ctttcctgaa
     7741 tacgtttttc attttccgca tctaaactgg cagtaatttc atctaaaatt aaaattggcg
     7801 catttttcaa aaaagcccgt gctatggata ggcgttgtcg ctctcctcca gaaagtgaac
     7861 ttccgttttc tccgaccaat gtatgataac cgtcaggtag tttttcaata aagtcttcgc
     7921 agttagctaa acgagctgct tttttcactt cctcatctga cgcttgacta tttccaatac
     7981 gtatattttc caaaatactt gtattaaaca aagttacctc ttgaaaaaca atagcgattt
     8041 tgctaaacaa agaagctgga gatacacgtt taatatcata cccatcaatt aaaatacagc
     8101 cttcatcata atcatacagt ctagacacta atttcaagat actagttttc ccggaaccac
     8161 tagcacctac taacgcagtc acttctcctt gtttagccgt aaaagagata tgatctaaaa
     8221 ttggggtatt gttatcatat gagaaagaga catctcgcaa ctcaatgtca aaagatttca
     8281 gaggactatc tgatccctct tggatactgg tttccttcat agctctaatt cgctgtattt
     8341 taggagctaa ataaaatatt tcaagcactg cttctttcat agaatcgaag gaatccttta
     8401 tttttattgc ggctagtaag taaccaacga cgtagagtat agtcacttca cccgttaaca
     8461 gtaagtgaac acctactaga atgactactg ctaaactaac atatgagaaa atagaggata
     8521 aggccatgac tgaaaaagta cttaattcta cctttaaatg aatcctttca ctctcttcca
     8581 tttttttgaa aagtctgtct tgaacttttt tagatagatt aaagctatta atctcttgct
     8641 gcaattctat agtttcttga aattcttccg agttttctct caaagtatcg taatatttag
     8701 tattggcttt cgtttgggat ttctttgata acaagattaa gacaaaacta aataacgttg
     8761 gcaaaataac agctaatccc attttgacat taccaaccaa aagcatcact gaaataaaag
     8821 ggaaaaacag agccataccg ccggctttag gtattgcatg actcatcgca tgctcaatac
     8881 cttcaacgtc agacatgata gtttgagata aatctgataa attatgtttt gaaaaatagg
     8941 ataatggtaa atttgacaaa tcgtctgcaa tttgcactct taaatgcgca ctttctttat
     9001 aagtactatt atataagttt tcatactccc tatctaacaa ccaatacatg aaaagcaaag
     9061 taacaaacga tacggcaaaa taaagccaac ggggctttgt attttctaaa actaattcgt
     9121 ctatcagcat cattataatg aatgctggta ccatattaat gcagtataca aaaaaactag
     9181 aaatagaagc tttagttaaa gcttctgcgc cctgatcact taaagcaaat ttgtttttat
     9241 aaaaactttt cattgtcaag cctccaatca tttgcattct cataattagt gaccaattgt
     9301 ttatacactt gatgtttagc cattaattca ttatgatttc cacgttctac tattttccct
     9361 tctgacaaga caagaatttc atctacagat ttaatgctgg ataatcgatg agctatcata
     9421 ataactgttt tttctttcat cagatgttga aaagcctttt gtagttcata ctcattatct
     9481 ggatcaatag atgcactggc ttcatccata ataacaatct ttgcatcttt taaaattgct
     9541 ctggcaatag ctatacgttg tttttctcct cctgacagat aaactccttt actaccaatt
     9601 aaggtatgct ctctttcggg aaatttttct aaaatcgatt cgcaaccggc taatcttagc
     9661 gcgtcaaata ctttacttgc tggcgcttga ggatttgcta tagaaacgtt atcgtaaata
     9721 ctctgattga atagtttcgt atcttgaaag acaaaagcaa ttgtgttaat aattgtgtct
     9781 tttgaatagt gatttaaagg cttaccacca attttaatct gtcccttatc cacattgtag
     9841 aaacctgaaa ttagtttagc tatcgtactc tttccgcttc ctgattttcc tactaatgca
     9901 taaattcttt tttgttctaa aactaagttg aaattttcta aaacacactc ttctttgtat
     9961 gaaaagctga cattttcaaa tgcaatgtca caatttttaa tctgtgtctc gttcccatat
    10021 tttagttggt cttgcttcat ttctaaataa agggagtcta gtgtttcgac tgcatatctt
    10081 gcttcaaaca agtgcatact tacatacatt attctcatga aagaaacaaa aataattcca
    10141 cttaaaaaaa acaacatcaa caagtcaaca gctaatatct tgggagtggt aagagaagtt
    10201 ataaaaaagg ttataggaac tatcaaaatg caaactatgc caaaaaatag ccattgaaaa
    10261 agaacaaaag gtgttttccc actcaaagaa tacttatatg caaacttagc ataatcgtta
    10321 attgctttat tcaaggcttt aaatgatgtt actttagcac caaatatctt tataacttga
    10381 attccgcgga tatattctac cgtttcagag cttaacacat ctaacgattc ttgatatttt
    10441 tgaataaaat ttgtattctc tgtcattttc tttaacaaga atacaccaat tactaacatg
    10501 gcgattagaa ccaatccaat tttaaagtta atgaaaaagc ctaaggcaac tgataaaaca
    10561 ggtgttacta tggcttgagc actatctgga atcaagtgag caattgccat atgtgtttta
    10621 gccgcgttgt catcaattgt ctttcgaata tatcccgaag aatttaaatc aaaaaaacga
    10681 aaacttgctt ctgttaaacc gctaattccc ttttttctca aattagtctc taagcgaaac
    10741 ccaaggatat gagaaaataa gccagaaaaa atataaaata aagctctaaa gttaaccaag
    10801 caacagtttg aaccgcataa ctcttagcta aatattcatc tcctacaata attagagact
    10861 gaagaaattt gtaaatactg taatatccat agactgttaa tacgacagaa aaatcagaaa
    10921 ataaagtggc taaccatccg tagtatttca ttttagggac ataactaaat aattttttgt
    10981 atactttcat tttttcctcc cccgcattca atttcttctc ttattcgttt acaactgtta
    11041 atgctttccg taatagcgta aattagagat gtacaaacag ataaaaggta aactacgttc
    11101 tattttttgt atttttttaa gacttttctt ccaatcaata caccaatcat agataaactt
    11161 gctacaacta acactgaaat gattccccat atacctaccg catagtggat cattgcatcc
    11221 attgttgcag cagattgtcc tttatctatc caatcttgac gaaaacgatc tgtaaagaac
    11281 catataggaa tccactgtcc ggcagcactg cctaattgca ttagagccca agacgcaact
    11341 tgaggaattg ctcgatcata tccaaataca gatagaataa tatcagctaa gatcgccata
    11401 ctaatcgaac tgataaacca aggtaagtac gccccgccca ttaacgtaaa aaataacaat
    11461 aaaacaattg tgtaaatagt aaaaattcct tttctagggt agttatagct aatataaaca
    11521 aatatgattc cagataataa tccaaaaatc ccgggggata tgctgtgacc aaacgcccca
    11581 ccaatactac taaccattat tgaaaaaaag taaattacta gggaaataac tgtagctaaa
    11641 ccaataaaaa tataatcttt tgttttcata tatattctcc tcaattctat ttcgttatgt
    11701 cattaaaaat ctgttctatc aaagttgaat cttctactct aagttttata tcttttttta
    11761 ttccaccatt tttta
//
LOCUS       DASJPC010000080        11667 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJPC010000080 DASJPC010000000
VERSION     DASJPC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.080"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..216)
                     /locus_tag="U4A13_002151"
     CDS             complement(37..216)
                     /locus_tag="U4A13_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780424.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="U4A13_002152"
     CDS             complement(597..1304)
                     /locus_tag="U4A13_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780425.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="U4A13_002153"
     CDS             complement(1297..1734)
                     /locus_tag="U4A13_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780426.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="U4A13_002154"
     CDS             complement(1749..2000)
                     /locus_tag="U4A13_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780427.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="U4A13_002155"
     CDS             complement(2000..2206)
                     /locus_tag="U4A13_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN1780428.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="U4A13_002156"
     CDS             complement(2643..2903)
                     /locus_tag="U4A13_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780429.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="U4A13_002157"
     CDS             2978..3181
                     /locus_tag="U4A13_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEN1780430.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="U4A13_002158"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="U4A13_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN1780431.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="U4A13_002159"
     CDS             complement(3810..4709)
                     /locus_tag="U4A13_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN1780432.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="U4A13_002160"
     CDS             complement(4722..5315)
                     /locus_tag="U4A13_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN1780433.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="U4A13_002161"
     CDS             complement(5391..5540)
                     /locus_tag="U4A13_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780434.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="U4A13_002162"
     CDS             complement(5556..7760)
                     /locus_tag="U4A13_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEN1780435.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="U4A13_002163"
     CDS             complement(7771..9231)
                     /locus_tag="U4A13_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN1780436.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="U4A13_002164"
     CDS             complement(9292..11664)
                     /locus_tag="U4A13_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780437.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DASJPC010000081        11555 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJPC010000081 DASJPC010000000
VERSION     DASJPC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.081"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U4A13_002165"
     CDS             complement(105..629)
                     /locus_tag="U4A13_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780438.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4A13_002166"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4A13_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN1780439.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4A13_002167"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4A13_002167"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN1780440.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U4A13_002168"
     CDS             complement(2351..3031)
                     /locus_tag="U4A13_002168"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN1780441.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U4A13_002169"
     CDS             3157..4026
                     /locus_tag="U4A13_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1780442.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U4A13_002170"
     CDS             4052..4567
                     /locus_tag="U4A13_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780443.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U4A13_002171"
     CDS             complement(4614..5207)
                     /locus_tag="U4A13_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780444.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U4A13_002172"
     CDS             complement(5232..5411)
                     /locus_tag="U4A13_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780445.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U4A13_002173"
     CDS             complement(5488..5997)
                     /locus_tag="U4A13_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780446.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U4A13_002174"
     CDS             complement(6093..6473)
                     /locus_tag="U4A13_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1780447.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="U4A13_002175"
     CDS             6803..7552
                     /locus_tag="U4A13_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN1780448.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="U4A13_002176"
     CDS             complement(7736..9013)
                     /locus_tag="U4A13_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780449.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="U4A13_002177"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="U4A13_002177"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN1780450.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="U4A13_002178"
     CDS             complement(9935..10879)
                     /locus_tag="U4A13_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN1780451.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..>11555)
                     /locus_tag="U4A13_002179"
     CDS             complement(10881..>11555)
                     /locus_tag="U4A13_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN1780452.1"
                     /translation="LSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQGMLEL
                     LGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGIFRMI
                     FAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCWFFGQ
                     VLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKLAKKY
                     YPGKGQ"
BASE COUNT         3286 a         2454 c         2043 g         3772 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaag
//
LOCUS       DASJPC010000082        11523 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJPC010000082 DASJPC010000000
VERSION     DASJPC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.082"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(125..670)
                     /locus_tag="U4A13_002180"
     CDS             complement(125..670)
                     /locus_tag="U4A13_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1780453.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(682..1374)
                     /locus_tag="U4A13_002181"
     CDS             complement(682..1374)
                     /locus_tag="U4A13_002181"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN1780454.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1393..1677)
                     /locus_tag="U4A13_002182"
     CDS             complement(1393..1677)
                     /locus_tag="U4A13_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780455.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1690..2253)
                     /locus_tag="U4A13_002183"
     CDS             complement(1690..2253)
                     /locus_tag="U4A13_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN1780456.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2481..3143
                     /locus_tag="U4A13_002184"
     CDS             2481..3143
                     /locus_tag="U4A13_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN1780457.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3267..4463
                     /locus_tag="U4A13_002185"
     CDS             3267..4463
                     /locus_tag="U4A13_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN1780458.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4564..5940)
                     /locus_tag="U4A13_002186"
     CDS             complement(4564..5940)
                     /locus_tag="U4A13_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1780459.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5977..6843)
                     /locus_tag="U4A13_002187"
     CDS             complement(5977..6843)
                     /locus_tag="U4A13_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEN1780460.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6932..7957)
                     /locus_tag="U4A13_002188"
     CDS             complement(6932..7957)
                     /locus_tag="U4A13_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1780461.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7975..8649)
                     /locus_tag="U4A13_002189"
     CDS             complement(7975..8649)
                     /locus_tag="U4A13_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1780462.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8833..9726)
                     /locus_tag="U4A13_002190"
     CDS             complement(8833..9726)
                     /locus_tag="U4A13_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN1780463.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9800..11497)
                     /locus_tag="U4A13_002191"
     CDS             complement(9800..11497)
                     /locus_tag="U4A13_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN1780464.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3421 a         2523 c         1975 g         3604 t
ORIGIN      
        1 ttaacgtaaa aagaataagt ttttttaaaa taacgtataa aaaagagcat aaaacaaagt
       61 cgtttgtttt atgctcttct tttattattt tgtgcttttt attttatcaa atcactttgt
      121 ttttttataa aatttctgcg cctagtgtgg actcaaaatg tctcaatgcc cattcatgtc
      181 caacgggatc gaaacttgcc acggcatctt tgaaaatatg gagtttgtat cctagattat
      241 acgcatcgac tgctgtatgc aacacacaga tatctgtaca tacgccggtc aaataaatat
      301 ctgtgatctg gcgttctcgc aaacggatgt ctagatctgt tccactaaaa gcggaataat
      361 gccgtttgtc tatccaataa acattttcct cttgctcata gtcttggtat aaacttttta
      421 aagagccgta caattcccgt ccacccgttc ctatcacatt atgcggagga aataagcggt
      481 tctccggatg aaaggaatct tttggatcat gcgcatcaat ggcaaaaacg acaaaatcct
      541 tttgatcaat aaacttcttt gtatgagcag tcagcttttt ttcaattact tgtcctgctg
      601 tcccagtagt cagctttcca tcctctgcaa caaaatcata tgtgtagtca atcgagatca
      661 atgcgttcat cttagtcctc cttatacgag atgtttgaca aattcgatca ccatttctgc
      721 tgatcgtttg cctgcgtctt cgataaattc gtcaaagctt tgtgtcgccg agtgatctgc
      781 tgtgtcgctc attgcacgta cgatcaaaaa tgggatattg aactgacgag ctgtttgtcc
      841 aacagcggct ccttccattt cgcaagctaa tgcttcaggg aaattcgtta agatttcttt
      901 gattttatct ggcgagtcga caaatgtatc acctgtaacg atcaatcctt cttttgcgtt
      961 caaattcgtt gcttttgctg ctttaaccat ttcactgcgc aaatatgtgc ttgcttcata
     1021 atatagaggc attcctggta attgtcctgg tttgtagcca aatcctgttg catctgcatc
     1081 aaaataagct actttgtctg atataacgat gtcacctact tgtaagcctt caccgattcc
     1141 gccagctgaa cctgtattga tcaccatgtt cacgccgtat tgttggatca acagacttgt
     1201 ggtaatagat gctaagactt ttcctatacc ggaacgtacg acgatcactt cgtggttacc
     1261 taatgaacct gaaataaata aagcgcctgc gcgttcccat gataatggtt cgcttaggtt
     1321 ctctctcaaa atttttactt cttcttccat tgcgccgata ataccgattt tcatcttttt
     1381 aattccttct ttctatataa atgcgattgc taaaaaaacc acacataaca ttaggataac
     1441 aataataaga gatttcatca aaaaagagtt ccatttggtc gtcttttccc gttcgctgat
     1501 ccttgttttc gtgattggtt tattcttttt gctttcttta cgataaaagc tggcaatctt
     1561 tttttcttct tgctgataag ctgtttctgc ttttcgctgt ttttttaatg actcgcttgc
     1621 ttgtgcttgt tgtcgcttgc gaagttcgct tcgcgtaata agcggtcctt tggccataag
     1681 ccgatccctc tactttcctt tggtcaatag ttcccagtat tgaatcgcat aaatcgtctt
     1741 tgcatcacaa atcaactgat ctttcatcgc ttgttgcgcc tcttcaagag ttagatgata
     1801 caactccagt acttcgtctt catcttgtgc taaaggattc tcgaccttct ctaccccttg
     1861 tgcatgataa atatgaagga cttcatttgc aaaacctggt gaaagataca tcgaagtaag
     1921 atggctcagc gacttcgcac gatagccagt ttcttcttct aattctctag ctgctgtcat
     1981 ttcgggattt tgtccttctc ctggatcgat ttttccagct ggaatctcca aaatcacttt
     2041 ttctaaaggt tttcgaaatt gtttgacaag aagcagacga tcttgttcgt caaaagcaat
     2101 gatcccgaca ccaccaggat gaaataccag ttctctcttc gaagttcctc catctggcaa
     2161 acgaacttcg tctacagcaa catcaataat ttttccgcta tagatttctt tgcgactgat
     2221 tgttttttct tcaaattgct cattgttcag catatcttct cctgctttct ttgtaatcat
     2281 aactgctttt ttttcatttt tcaatttttc tcaaaaactc cttattagtt taccatagat
     2341 aaaccttaga ttttctctaa atatagaaaa tccttcttta gtccgatact ctttctagta
     2401 gtccgtatgc taaaataagt atgtaatcga ataacgaaaa gaattcaata ctcaacgata
     2461 aatgacatgg agggtttatt atgataaaac gaaattggcc gtggcttcta gcattggtct
     2521 tgctatttct ctttgtccga aacaatagtt tttcttttgt tctaggactt ttattgatag
     2581 gtgtagtttt agttttgtgg ggactctttg gttcgcgcag gaaaaaggga aaacaagaag
     2641 aaattcctgc actgactgac gaacttgaaa ctcactatga aaaaagcggt atgacagcga
     2701 atgaaatttc attttttcga caaacaatga atcaaacaaa aaatgagatc aatcaattgc
     2761 aaatgaatat gcagcagaca gccaaactta aagcaatcga tctccgccat gatccagtca
     2821 aagcagcgaa agctttattc aaagaattgg tgaaagaacc aaaccgactg catgaagcaa
     2881 gtcagtttct ttatacgcat ctgccgaatc tagttgattt gacaaataag tataatgaga
     2941 tcaatgacca tgagatcaaa aacaaacaga cctatgaaaa actagaagaa agcgcgcaga
     3001 tcatcgacca gctgtcagct ttaatcgcac aagattatca aaaattcgtt gctgaggatc
     3061 tagatgatat cgacgtagag atatctgttg cgaaacagag cttaaagcgt gataataaat
     3121 atgaaccaga acaaaaggat taactctttt gttcataaaa gaagactcgg aacattcaga
     3181 tgatgtctgg ctgtttcgag tactttttct aaagtagaga cattcatgta agaatcgaga
     3241 acagattcaa caaggaggaa ttttttatgg atcataaacc gactgaaaca aaagatgtta
     3301 caccagttaa cgatacactt gaagatttat tgaacaaccc gttttcaact ccaacagata
     3361 cgctgaccac ttcacaacaa gcggagattg atcagctcca agagcaacag acagctgctc
     3421 gtttaattga caaactaccg gcggaacgcc aagaacaagc taaacagcta gcagcaaaaa
     3481 tcgatgctaa tgatgcacag tctgtcatca gctacggatc ggctgcccaa gcaaaattaa
     3541 gtgaattctc acaatcgatg ttgaatcatg tgcaagcaca agatattggt cctgtaggag
     3601 attccttaac tgaactgatg tatcgtctcc aagaagccaa tcctgacgaa ctgcgtgccg
     3661 gagaagggaa catcttccag cgtgtcttcg ggaaggtcaa acaatcgatc tatgaagtga
     3721 cagcaaaata tcaaaagatt ggtgcacaaa ttgacaagat ttcagtgaaa ttagataaag
     3781 aaaaagacgg tctgctaaaa gataatctga tgttagaaca gctttaccag aaaaacaaag
     3841 actactttga tgcactgaat atttatatcg ctgctggaga attgaaaatg gaagaacttc
     3901 aaacaacgat cattccagaa gcgatgaaaa gagcagaaga aactggcgat cagatggatg
     3961 ttcagattgc caatgattac acgcaattcc tagatcgtct ggataaacgt acgcatgatc
     4021 ttcgcttagc tagacagatc acgatccagc aagcaccaca aatccgtttg atccaaaaca
     4081 caaatcaggc cttggctgaa aagatccaag catctatcgc tacagccatt ccgctgtgga
     4141 aaaaccaagt cgtgatcgca ttgaccttgc ttcgtcaaaa agatgcagta actgctcaac
     4201 gccaagtctc tgaaacaaca aacgacttat tgaagaaaaa ttcagaaatg ctgaaaatct
     4261 cagccatcga aacagcgaaa gaaaatgaac gtggaatcgt ggatatcgag acccttcaaa
     4321 ccactcaaaa tgatttgatc gaaacaatcc aagagacatt gcgtatccaa aaagaaggaa
     4381 aagaaaaacg tcgtcatgca gaagtagagc ttggacacat ggaagaagat ctgaaacaaa
     4441 aactgttgga tttaacccaa tagatcacct ttcgcttgtt actaaaacaa acgcccaaca
     4501 acagtttatt acaactgttg atgggcgttt ttcttatctt tcttctaact tctccattgg
     4561 agtctatttc tcattcatct tgctgtgtcc atagccatag ccaaaataaa tgatcagtcc
     4621 aataaccatt acgataccga acacgatcca tgtcacagtt tgcagacgaa gcatcaatac
     4681 aatacaagtc aagatggaga cgattggcaa tagcggaacg aatggcactt tgaattctcc
     4741 agcttttggt ttgccaaaca tttttctcaa cttcagtaag cctagactaa caagcatcaa
     4801 ataggcgagc gccatgatat tcgttagttc cgctaataca ttcaatggga aaaatccggc
     4861 aaagaataca gctgccacac cagcggcata agtcgctttt ttcggtgtac gatgcttttg
     4921 atcgatttct gtcataaact taggaagtaa gccatccttg ctgacagcaa acagtaaacg
     4981 agcaagtgaa tacatcatcg agatcgttac cgtcagtagc gttaaaattg cccctacagc
     5041 gatcaccgcg cccactgttc catgtcctac atacctcatt gcaaaagcca ctgggtcatt
     5101 gactcctagt tcagtatacg gaacgattcc tgtcaagatc agcgtcacga tgatgtaaag
     5161 gacagtagca atcagtatcg aaccaatgat tcctcttggc acatcttttt gcgggttttt
     5221 gacttcctcg gctgccattg caacggcatc aaatcccaag aaagcaaaaa acacaagagc
     5281 tgcaccatct agcacaccgg aaaaaccaaa aggcatgaat ggctgccagt tatcaggttt
     5341 aacgaaaaat attcctacaa tgataaacaa cgcgatgata ccaaatttga catataccat
     5401 ggcattattc aaacgtaatg ctttcttggc ttcaagcgac acgatgtaag tgacgaatat
     5461 cactacgagc accgcaatca agtcaatgta tgtaccattt tccggattgt acccgccagt
     5521 taatgcttgt ggaagttcgg cccctaaact tttcaaaaaa ccttgcatat agccagacca
     5581 tccggaagca acagaggaaa ccgctagtaa aaattccccg atcaacaacc accctgtcag
     5641 ccaggcagca aattcaccaa atacaacata cagataggca tatggcccac caatcactgg
     5701 cactcgcgaa gcaaattctg cgaaacttag tccagacaaa atgacaacga ttgcagctaa
     5761 gacaaatgaa agtgacagtg ctggaccagc attttgattg gccgcaaccc ctgtcaccac
     5821 aaatattccc gtaccgatga tcgcaccaat tccaagcatg atcaaatcag acgtttttag
     5881 ttcttttttc atctcactcg tatgattgag attgatctct ttcttacgaa acaaatccat
     5941 tgaagttcct ccccatttta ggttggaaaa cagcgattaa actgttttca tataccctaa
     6001 aaggatacca cctaaaatga ctaaaaggca accaacaata acataaatga gctctttttt
     6061 cgtttttctt tcgccaagga tgaaaatccc acctaaagta gagataatga tccccatttg
     6121 tgaaagtgag aagccaacag ctaaaccaag tgatttgact gttagaagca tgcagatgtt
     6181 tcctaagccc cataataatc cacagatcat gtttttccac acaaactgat cgactttaac
     6241 tttacggaaa gcaaagaagc ttgcgcctaa gatcattccg atactttgag gtaagataat
     6301 tgacatcgca tccaaattcg cccagttgac gatgatggta tatacaccat aaccaatcgt
     6361 agagaagatc aatgctctaa aacccttacc aaaatcaagc attttattat ctgtatctat
     6421 ttttccttcg tcatcttgtc gggcagtcaa atacgcaccg cttacaagaa gagctaaagc
     6481 aataataccg tatacataat ctttcgtttg tgtccattca tggaagaaaa cggctcccgc
     6541 aatcgtattc aaaattaatt gaaaaccagt agacaatggt agccctacag aaacacccat
     6601 gtacttcatt ccatggaact gtccattttg tccaacactc caagctaagc cggataaaaa
     6661 tccgatcaag aaaatccaag gggtgattac aggactaaca acgaaaaaga caactaaaga
     6721 aaacagaaat gcaccgatcg tcattcctag tgtttgttga ttggcatcgc caccaatttt
     6781 accacttact aaaccaatac taccccatgc caacataggg actaatgcta ttaaaatttc
     6841 cataaccaat ctccttttat ttcacatgat aagaaactta tcagcgaaaa cgttaacaac
     6901 acaagcactt tttttcgttt ctaacgattt tctaacgttt ttcattataa aaatttaaga
     6961 ttgggaaaaa taaagtcatt gttgtccctt ttccttcagt tgattcagcc gtcaaatgga
     7021 tacctaattt ttcggccaga ttttttgcta aataaagtcc taatcctgtt gaatgtgttt
     7081 tgctaagtcg accatttctc ccagtaaatc ccttatcaaa gatccgtttc aagtcttccg
     7141 ctgggatacc gatccctgaa tctttaactg aaaggctgac tccttgcgct tccttttcta
     7201 tgctcacaat gatgtttccg tgatccggcg tatatttgat ggcattacta agcaattggt
     7261 tgaagataaa agcgatccat ttagcatcgg tcaaaacagt atgatcctca ccaacgatct
     7321 cgtagcgaat atttttttgg ataaaataat ttgcctgact gcgtatcaca ggctgaatga
     7381 ttccttttag tccatattct tggatgaggt agtcccgcga gaaactgtcg agtcttgcat
     7441 aatacaaaac ttgttctacg tattcatcga tttttgataa ttcattttct agtaatgtat
     7501 acttttgatc gtcaatatcg aactcgatcg aacgaactag caatttagaa gcggaaagtg
     7561 gcactttgat ttcatggacc catgagtcaa tataatcttt ttggtcttgt tgattactga
     7621 ttgctgattg cattacttct tggtgttcac ggatcagctg attgatatat tcttctacca
     7681 gtttttcttc ttctgtcctc ccaccactca aataattttg caaaggggaa gttttttgta
     7741 tgtcggctaa ttttccccac catcttctct tgctcaagta accacccagc agataaaaaa
     7801 tcagaaagac gccctcaatc aaagctaaat aagcaattgt gctcaaatca actttgatgt
     7861 ttggcgccag ccacatcaca aagatcgtca atgcaataaa aaacagccat cccaagatca
     7921 atgtccactg atctttcaaa tatttaaaaa aattcatctt aaccgctcat cctttcaggg
     7981 aacgatatat ccctgtccta cttttgtttc gatatatcct tggatacctg cctgttcgat
     8041 ttttcggcgt aaccgattga tgttgactgt caatgtattg tcatctacaa atcgttcgtc
     8101 atcccacagt gctcgcagca atttttcccg acttaagatc tttccattgt gcttcatcaa
     8161 aataaataat agacgatatt catttttgct caaatcaatg atctctccgt tgatttccat
     8221 acttccgttg tcaacattca acgtaatgcc attgtggctc atcatttcac tagataattc
     8281 tgtgtagttg taagaacggc gaagtaatgc attgattttt gcaatcagta catcgatttg
     8341 gaaatgtttt gtcacaaaat catctgcccc catgttcata gccatgatca tatccatatt
     8401 cgtattcctg ctggagataa aaatgatcgg tactttcgat acttcacgta ttttttgaca
     8461 ccaataatag ccatccaaaa cgggaagatt aatatctagt aatacaagct gtggttcttc
     8521 tctttcaaag tctgagaaca cttgattgaa atcggtcgtt ccaaaggttt caaattgcca
     8581 tttctcaagt tcctccatga tcaattgtcg aatgatctct tcgtcttcga ctaccatgat
     8641 ttttgccata aaaaatccct ccatctttct tcttgctact acaatatcaa agaagccctt
     8701 gtgaaacaag ttggcttacc tggaaaatgt cattttagca aacgttaaaa aaaactggaa
     8761 atatgcgatt taatcgcgtt tgtctcttcc aagcagctta aatttattat gttattctta
     8821 ataattggtc tattatctgt tatttttctt tcgttgtttt tgtcttttaa attttcgata
     8881 acaccaataa ccgaaaagcg ttagcactag caatatacct agaaggagta taagtaatcg
     8941 ataaagatgg tatttttttg tctgttctat ctttgaagag acttcttctg taaagggaac
     9001 tcgtcttcct gtcactagta atcggtgagt gttcacggtg taaggtgtac aagtgataag
     9061 tgtgactaga tcctgctttt ctctaattgc taatgtattt gtttttgttg gaaggacaac
     9121 aattttctca acgacttcat aagcgagcat ccgaccatac acttcaagat agaatcggtc
     9181 atcttgctcc atttcttcaa gatccgtaaa gagttttttc tcagcaatac cgctatgggc
     9241 ggtaatcacg gaatgtgtac tctctcctcc agttggaaaa gaggtgcctt gcaggactgt
     9301 cgctcctcta tctaacaaaa gatcattcgt ttcatcaaat aaaggcaagc taacattgat
     9361 tttcggaata tagatggctc cgatcatatg ttgttcgtaa tattcttttc ctggattttg
     9421 gacatttcta accgcttgct caaaaggatc tttgacttgt cccattcctg ggattactga
     9481 tgtatgagca agcgtctgat ttttcttttc atgcactgct aattgttgct catgtttttc
     9541 ttctttatcg cgttcaagct gcttttgata attctcgatc cttctttgat ccaaataatt
     9601 gctgagagca tctgctaaaa atggatatgt aaaaactgca gctccggcaa gaaacatcac
     9661 tgccataaat aattgtaata aaagactgat ttgggtttga ggtctattac ctattttctt
     9721 tttcattttc ttcaccttct tatttaaaaa aactgcagca acctttataa aaccgattgc
     9781 tgcagcttat ttttgttgct tatacttcag acttcatttt agatttacgg taccagctgt
     9841 acgctccaat catcaaactt attccgatta gtaagaatgc caagagcccg ttcccaccag
     9901 tagcaggaag tgacccgcgt ttcatattcg gtactctttc tggccctgga ttttctactc
     9961 ctacgatctg aatttcttca ttatagcttc cataagagat gataaattca gtaaaggctg
    10021 cctcacctgt tggaagtaca taaccttctg gcgccatggt ctctttcatt tgataggttc
    10081 cataatctaa cccttgaacc tcaaagtatc catttccatc tgcgacaaat tctgttgctt
    10141 gtccgcttgt tgcccactca attctatctg ctcctgattc ataaacagct aaaggcgact
    10201 cccctttata aaatattgcg tattcagaac ggtcttcatt ccacaattcg aatcttgcat
    10261 ctttaagcaa ttcactgctt gatgcatcat atttttcaaa ctttcttcca cctgttgtga
    10321 ctttctctgg cggctctact ccttcttcat aatcaaattc atcatttcgt ccgatttcaa
    10381 taaccgctgt gttgttatgg aaatcatccg cttctagttc tgtggaaacc ttcaaattat
    10441 acgtgatcgt taaatctcct ccacgtgcat ctgctaatct acctaaagta tctactttgg
    10501 ctactccatt ttcttctgtc aaaatcgtca cgacaaaccc gttgcttact acttctacta
    10561 tataatcttg tccttttgtt aaaatcgtct cgccggctcg cacttctatt tcctgatcat
    10621 aataagttag accaactgta ggcgtatctg tgattttgta atttttgata acagcgcttc
    10681 cgttttcaag gacagaatcg atgaaataag gaataggtgc tgtgatcgtg taagaagccg
    10741 tttttcctct ttctagatca atataagaaa tcgtttcttg ttctccagct tcattaataa
    10801 tcgttacttg tctaaggtcg ctttcctcta cattcatctg tttatctgct tctctgattt
    10861 cgtttttcgg atacaaatgc acatctgtat tgtatgtgtc tttccatgta gaaccatccc
    10921 acacgccatc caccatatca ggcatcatca ctggcatcat aacaaccatt ggagacgcga
    10981 ttctggaaat atgagctggc gaataagttt ctaagaaaag atagacttta tctctagaag
    11041 aaggtgcttc ttccgtcact tggacggtag agaataccgc ttctccagct tcattcgttg
    11101 tttgtttgtc aatacttgtc agtcgatatt ttaaaaacca actttctgtg tgccgttttt
    11161 gaatccattc aatcgcttcg gcagaattaa gaccgtcttc tcgttccggt gttaaaggat
    11221 tatctcgaat caaatcgtaa tatacttccg ttacgtcaaa caaatcaaaa tcgactcctg
    11281 gaatattttc ttcaccaaaa tccgtagaaa ccaatccgct attcggtcgt tcttccggaa
    11341 cagatgagaa accttttttg tgtaatgtaa tcgtcacttc acttggtttt tgtgcgactt
    11401 cttcggcttt ggctagtttt ccgttcccga aaatccctat tcccgcagta acgagtaaca
    11461 gtacactcat tcccgtgaca atcatttttt tattcatttt tttctccccg ttcttttttt
    11521 att
//
LOCUS       DASJPC010000083        11453 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJPC010000083 DASJPC010000000
VERSION     DASJPC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.083"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..415)
                     /locus_tag="U4A13_002192"
     CDS             complement(<1..415)
                     /locus_tag="U4A13_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780465.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYS"
     gene            complement(478..723)
                     /locus_tag="U4A13_002193"
     CDS             complement(478..723)
                     /locus_tag="U4A13_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780466.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            868..1707
                     /locus_tag="U4A13_002194"
     CDS             868..1707
                     /locus_tag="U4A13_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1780467.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1858..4875
                     /locus_tag="U4A13_002195"
     CDS             1858..4875
                     /locus_tag="U4A13_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1780468.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4939..6402
                     /locus_tag="U4A13_002196"
     CDS             4939..6402
                     /locus_tag="U4A13_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN1780469.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            6869..7249
                     /locus_tag="U4A13_002197"
     CDS             6869..7249
                     /locus_tag="U4A13_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEN1780470.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            7246..7515
                     /locus_tag="U4A13_002198"
     CDS             7246..7515
                     /locus_tag="U4A13_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780471.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            7515..8435
                     /locus_tag="U4A13_002199"
     CDS             7515..8435
                     /locus_tag="U4A13_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780472.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            8457..9329
                     /locus_tag="U4A13_002200"
     CDS             8457..9329
                     /locus_tag="U4A13_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1780473.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            9348..10520
                     /locus_tag="U4A13_002201"
     CDS             9348..10520
                     /locus_tag="U4A13_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN1780474.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            10531..11181
                     /locus_tag="U4A13_002202"
     CDS             10531..11181
                     /locus_tag="U4A13_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780475.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            11197..>11453
                     /locus_tag="U4A13_002203"
     CDS             11197..>11453
                     /locus_tag="U4A13_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780476.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
BASE COUNT         4176 a         1915 c         2404 g         2958 t
ORIGIN      
        1 gtgagtaagc aaagtattgt tcatttactt tagccaaacc aaacgtatcc ccaatcttta
       61 acttgttagg ggtatattga atgtcaggaa tggagaataa attttctttg gtatttgtta
      121 ctttgataga aaattcaagg tcagttcctt ctttaggagt aataaacgca atatttttct
      181 tttcaaataa gacagtagta atttgagaag aaccaagatc aattaaatct tgtccatgcg
      241 tttcttttaa actatttagt gaaggataga cggaatgatc gctagattga cttgtagtag
      301 tcgattgaac cgtattttgt ggctgtgttt ctgttttgtt ggatacagaa aaagcaataa
      361 tagcgagtac aatcagtacg attgaaccaa ctgaaacaat gtatttctta ttcatagaac
      421 ctccgttgta aaagtgttta ttattgtttt acttgtaaat ccaagacaga aaacgcctca
      481 ttgacgtttc tgtctttttt cttcgagctg ttttgcgtat ttttcaatat cttctcttag
      541 atataagcga atgatggaag gaccaggttc gtcgcctatt tctagaaatg gtttgatatt
      601 atttctacgt aaagagctag aaaaagcatc atagttttgt ccagtgattt tggcagcttg
      661 tttacgatta agtaaattat tttgagccca atcatgtatt tcatttaaat tattaagttg
      721 catgtgtgac attcctttta gttattttag taccaaagtt agtatatcaa aaaataaaaa
      781 aatctacaaa aaatatatcg actgatagac ataagtttgg tactatgttt ttgtaaataa
      841 atttattcaa atcggaggcg aaaaacaatg aaaatgttga aagtattggc actaggaatc
      901 gtagtcggag gagttagtct tgctgtggga acgaatgctg aagcagcaga aaacaaagtt
      961 tggacacctc gttcggcgga acaaatcaaa gcagaaatta aagggaatga atacacaatt
     1021 gtctggggag ataccctaag cgcaatcagt caagcaacaa atattactgt ccaaaaatta
     1081 gcggacatga ataagattac aaatgtggat ttaatctatg caggaaataa attagtattt
     1141 aatggcaatg tagcaagtgt acaaaacagc gcaggtgaaa ctgtggctca aacagtgatt
     1201 caagagaaag acaaagttga tccgaatcaa ccagtaggac aaggaacgac ttcaaataca
     1261 caaacgacac caaatacaac gacaccaaca acaccagctg gagatcaaac aggaacaact
     1321 ggtggtacaa caacgccaag tacaccagaa aacaatggtg gcactactca aacacctaca
     1381 caaccaggta atggtggaaa tactggagga gaaacaactc cttctaaccc aagcacaggt
     1441 ggaggaacaa caacgcctga agaaccaagc aatcctggtg gaggcgatca aggcggaaat
     1501 ggtggcacaa cgacacctga agagccaaca attgtagttg gagagattgg taatagtgga
     1561 aaggtattta acactgtaga tgaagcctat gattgggcta atgcagaggt tagcgatcca
     1621 aattcgcagt ggtacaacaa aggttttatg gtggatgtta ttatttacag tgacgggtca
     1681 caaaaacaca ctgtagattt ctactaaaaa taaaagaaaa gtaagtggtt gatgaagact
     1741 agaattataa gttctagtct ttttttgttt tgaggaggat aaaaatgttt cggtgtcgaa
     1801 gtccataaga aattacttta gaagactgaa aaataaaatg aaaggaacga aaaaaatatg
     1861 tttaaaagaa tggcaacttg gttggcaatt ttcgttacat ttctcaatcc catgttagga
     1921 tctgtaacgg catttgcaga aacgataaat gaatcaaatt ctactgaaga agtaatgaag
     1981 actgaagaat caaaaagtag tgaagaagcg atcagtactg aaaccacaag tgaaacaaca
     2041 gaagagacaa ctacgcagga cacgacagaa gatgtttctc cgcccaatgt aatgactcaa
     2101 gaggtccctg aaggttatga gaaatacttt ataaccattg aagatattca aaaagcgact
     2161 tctttttctg aacaagaacg attggcgcaa gaatctgaag aatggcaagc aatcttggaa
     2221 aagaatgcac agacacgtgc taccaatcta acattgactg aaaatggtcg taaagatgtg
     2281 aatatgactc gaattactcc tgaaggaaca atgtatactc atgattacat ggttcgctgg
     2341 caaattgacg gagaagatgt tttttgcatt caagaaggcg caatcacgag tgcgggtatt
     2401 caatatgatg tgacaccact cgaaaattta attacgacaa gtgctattag aaaaaagatt
     2461 tctttaattg gttattttgg ctactattct agtggttcaa tggataacta tgtattagca
     2521 caaacatata tttgggaagt tttaggtggt tcatttgtta attatggaac tattggacaa
     2581 tctaattatg aagctttcaa acagactgta gatagaaaaa taaacaattt acttgaagat
     2641 ccttcttttg atagccaaac agtgacggtt aaagtagggg aagaagtaga agttaaggat
     2701 acaaaaggtg tgttttctca atgggatggg tcggttgttg aaaacacagc taacgttcaa
     2761 gtaagaaaat caggaaatag tttgtttata aaagcaaatc aaaactcaaa tgacggacga
     2821 atttctgttt cttcactgga tccaaattct agaggggtag gttcaagttt tgcctatgta
     2881 gatcctgttg ctcaaaatct aggaaagctt tatgtgccag atcctaatag agctggacta
     2941 actattaaag ttctaaaaaa tggcgacttc aaactaacca aactagacgc tgtcacaggg
     3001 aaaaaagttc caaatgtgac gtatgaaatc aaacgtcatg atacgggaga aacaaaacaa
     3061 tatacaacgg atgccaaagg ggaaatcgcc ttaaaagaaa tccctgcatt ctatgaaaaa
     3121 gacggaaaat atgttcaaaa taaattggat gtcaaagaag tcaaagcacc agctgggtat
     3181 attctagaca aacaaactca tacgattacg attgaaccaa acaaagaagt gacgttaact
     3241 gcaaaaaatg acttacaaca gttcattcca gaaggaaaga aagtcaaaga agtctataat
     3301 gcggaagaat cctggaatca aggcaaacct gtttatgata ccgttcctgc agaaaatatc
     3361 gaatttgata ttatcgccaa aacagatatt actgcgccag ataaagaaac agtccttgtg
     3421 aaaaaaggag aagtcttgga ttcagtaaaa actgacaaag acggacactg gaaggcaaac
     3481 aaaaaattct acaatggcga tcaaaacaaa taccaattga ttgaaaaaaa tgtgccagac
     3541 aactaccgtc aaccttctga agaacaaacc acgttctctg tggcctatgg cgataacacg
     3601 acgcctgtgc ttacgtttga attagatgtg attgacaact tgttaaaaac gggtgaagta
     3661 gacttcaata aagtcgattc aatcaatcat ctacaattac caggagcgaa attcctgatt
     3721 gaaggtatga ctgcacataa taaaaatgtg tcctttgtgt atgaaacaaa agaagaacca
     3781 gaaaaactaa aattaccaga cggtacgtat aaatttacgg aaattgtgtt ccctgagcat
     3841 tatgggcaag cgtcaggaca aacagaaacg attttagtaa cggtcaaaga cggagaagta
     3901 caaccaatta ctgtgaaaaa cgaagaaatc gtggttccag aagaagacca accagaaatt
     3961 tctactttgt ttgtgacaac gacaggtgga aaagatttca acccgaaaaa agaccaagaa
     4021 ttagtggata aagtgtccca agtattccct aaatctgcgg ttgggacaga gaaatattgg
     4081 gtgacgcaat tccataaaat tactgccgat ggaaaagtca ctgtattaga tacggtaaaa
     4141 gaaacacgaa aagtgaccga tgaaaaagaa acatttgatg tgacgttcaa ctacaaagca
     4201 gacatggttt cagacggtga cacgattgtc gcaacgcaca ttgtctataa cgacaaagaa
     4261 gaggaagaag aattcgctcg tcattttgat ttaaccaata aagatcaaac agtgactgcg
     4321 aaagaaccaa aaatcgaaac acgctttatt acagtgaatg gcgaccaaac atttgatcca
     4381 gacaaagacc aagagttgat cgatcatgta tcgcaagaat ttccagaatc tacggtaggg
     4441 gaaaccaaat actgggtgac tcaattccat aagattgata aaaatggaaa agatacagtc
     4501 gttggtacgg tagaaactgc acgtaaagtt tctaaagcgt ctgaagagtt taaagtatcc
     4561 tttgactaca aagcaggcac gttaaaagac ggggagaaat tggtcgcaac tcatattgtc
     4621 tataaggata aagaccataa agaggaagaa gcacgtcact ttgatttgaa gaatgaaaaa
     4681 caaacattga cagcaaaagg cattaaaaaa gaaacgccga caacccctgt caaacaaacg
     4741 ccgacgagca caccaaaaca aagcttacca aaaacaggag aaacagtgaa cgatgtgtta
     4801 attgcattgg gactgtttct ttttgttggt gtgggggctg ttctggttcg tcgtaaaatg
     4861 aaacaaagca attaaagtat gactagagac agcaatcagc tgtctctaga tgattgattg
     4921 aagaatggag gcaatcttgt gaaaaaaacg agtaaatgtt tatctgcttt gttattagta
     4981 ggtcaagtat ttgggtatac gccagcggtt ttggctaatg aacaaggcaa caatcaactg
     5041 acaaattcgc aatctgcaac gatcactgaa gaaacgacaa caaattccac tggagaagaa
     5101 aatcaaccag aaacaccgac tgaagataca atagatacac cagataatcc agaaatgaac
     5161 gctccaccaa caggttctga agaacaacca gaaacaccga atcaaccaga caaaccagaa
     5221 gacaatcaac aacctgagga ggaaccgtca gaagaaccaa atgagcaacc ttctgatgaa
     5281 agctcacaga ctcctcaaaa accaagtacc gaaacagaag cggaaccgcc aacaaaaacg
     5341 gaacgcccac aaaaggcacc tgaacagagt gtgactgtac ctatgaatga cgtaccgcag
     5401 gcacaatcaa tcggttcaga agagacaaaa aactaccacc aagcaccaag cgcaccgata
     5461 caagaattag tcccttccag cacacaagat aacgaaggga ctattcattt tgaaaaagac
     5521 gcgtctgtag aaacgtttat ccgtaaaatc ggtgaatctg ctcgtaaaat tggacaagaa
     5581 aatgatttat acgcttctgt tatgatcgcg caagccattt tagaatccgc aagcggacaa
     5641 agtcaattgg ctcaggcacc aaactacaat ctatttggaa tcaaaggcac acataacgga
     5701 aagggcgtct cttttgcgac acaagaagat ttaggcaatg ggacacttta tacgacccaa
     5761 gcaacttttc ggcaatatga gagctatgaa gaaagcttaa aagactatgc caaactgtta
     5821 aaagaaggct tagttggaaa tacctctttt tatgatggtg tttggaaaag taaggcaaaa
     5881 acctatcagg aagcgactaa atttttaaca ggaagatacg caacagacac tagctacgat
     5941 aaaaaattaa atgggttaat cgaaacctat gacttaatca aatatgataa agaagtagct
     6001 ggtccacaac taagcaaaga aggctatatg gtacctctaa aaaattattc aatttctagt
     6061 tccttcggtt ggcgaggaga agaatttcat agaggattag acatggcagc caatcaagga
     6121 gaaccaatct atgcgagcaa ggcaggaacg gtcatagaag cttcgtatca tactttgtca
     6181 ggaaattatg tcacaattga gcatgaagac ggaagtgtgg ctctttatgc gcaccaacag
     6241 gaatattgcg tgaacgtagg acaaacagtt gaacaaggac aaattattgg ttatgtgggt
     6301 tcaacaggaa atagtacagg accacattta catttagaac ttgccttaga tggtagttta
     6361 acaaaagaca aattaatcaa cccagaaaca gtcttattct aaagaataag gctgtttctg
     6421 ataaggagtg gtatagaaaa tgtggttact ttttaaatat ttgatatatt tgtatctctc
     6481 attttcgttt tttaatttga aatctcgtat ctatctagaa cgtagtttat taaatatagt
     6541 attaggaata ttttttatcg tttgggtctt cattgactta tttctttact caaacgattt
     6601 ttattttatt tataaagaat ttaaaaatca gtaattgctt ggagagttaa catatattgc
     6661 ataaaaaggc aaagtatgta catggtattt atcgtgttca tactttgcct ttttatggcg
     6721 aaatttttta gaaaggagga aaaaattttg ttatttgtaa gtggatttct agctggtggt
     6781 gtattggcca ccttttttat ggcaagtttc attgttagta aaacagaaga aactagaatc
     6841 aaaaataaac attagaaagg acaaagatat gatcacaaat tataaaaaat tttggcagaa
     6901 tagctttaat tacaaaggga aagccacaag aaaagagtat tggctcgttg cgttaatgaa
     6961 tgccctcatt gggtttggcc taggcttagc gattggacta gcaggagcgt tgaatgcttc
     7021 tgaaacaata attggttgtt tgataggcgt attgattgtg tattgtttcg tagttcttgt
     7081 cccgtctctt tctctcagcg ttcgacgttt acgagacgca ggttttcatt gggcatttat
     7141 ttttttctat tttgtcccaa cgattggctc tcttgtgtta ctggttcttt attgcttacc
     7201 gacaaaagag tcaatagaag ataaacaggt acaggaggaa accagatgat tatagtaaca
     7261 ggaattgttt tattagggat aggcgtttta tttggctatc atttcgccta ttttccaaag
     7321 attggcgaat ggcaacaaag acaagcgatc caaaaatatt ttcaagtcaa aggcaacgaa
     7381 tatacctatt atgctgaaga tgaggatagc tatatcgtca gtttgaacaa tcaagaatat
     7441 cgggtgaaat tttctttaaa taaccctatt caagtggtgt atgcggtaga agtggtggaa
     7501 gaagtgtttg actaatgatg atgattcgat ttacagaagt tgataacatg tctttactaa
     7561 cagataaaaa aattgcatta gataaggcat ttattcaatt gaaaagagaa aatgaagagc
     7621 tattggcacg agcggaaatt ggctggtgta ccttggaaat ctacacagaa aaccatgaaa
     7681 ttccagtatt tgaaggctcg tatgattttc cagtagaaga gtttcatttg atttcattga
     7741 ttgaacaatc tttaacagaa aatgggcaat taagtgaaga agcacaaacc tttattcaaa
     7801 ccttaaattc cgaattaccg aagttttatc aagtaaagaa agaccaaaag aacgaacgtc
     7861 gaccaacaaa aatcaaaaag gaaaagaaaa caccactttc tgtagaaaca gcgtttacac
     7921 aaagcaacgg ctctcttcag aagaaaaaaa tgtcttttca atggcttttg tttggattta
     7981 tcgtcgttgt atcttgtggt ctttttatta tgcaactttc aacgcaaaga gagattgaac
     8041 aactaacgaa aaaggagcac cagttacaag aacgcattgc atttgcggaa gaacttgcga
     8101 caaaacaacc acaaattgat acatttagtc gttattttat tacgaattat tattcgggtg
     8161 aaatggcaga agaacggtat aggaaaaagc ttcaatcgtt tctaacggaa aaagttgcga
     8221 aacaaatcag tcctcaaaag gaaaaagtca aaagtatttt atcctgggaa atgaagaaag
     8281 aaggcaagca ttggcaaatt gcgtatgtca ttacattgga aaccgagaaa aagacacaag
     8341 aaacaaaaaa agtgtctttt caattggaag aacaggaaaa aaattatcta gtttccacgc
     8401 ttcccgaact agatgatttt gaaataaatc aataaataag agaagaggaa aaacacatga
     8461 aaaaaatgat tttaggaagt ttatttagtg caactgtatt gctaggtatg gtaggaacca
     8521 gtgcgtatgc agatactacg tcaccaggtg atccaagtgt accaccgaca gaaattattc
     8581 caccaacgga agaaacacct gaagttcctg aagaacctgt tttaccacca acagatggag
     8641 gagaagaaac cccagaaatt cccgaaatac cagaaactcc tgaaacccca gagattccac
     8701 caacggaaga aaatccagat acaggtggag gcacagaaac gcctgaagta ccagaaaatc
     8761 cagatactgg cggaggggaa ggaacacctg aagttccacc cgttgaggaa aacccaggaa
     8821 atggtggtaa tacagagaca ccagaaaagc cgtcgaatcc aggaggcaat gaaggaaata
     8881 cgactcctcc aacgacacca cctaccaata atggggggac gaacactacg cctccagtac
     8941 aggacgtcgt agtaaaacca gatggaagta ttggcagcca acaaacaggt ggacaaaatg
     9001 ttcaaattga aaccaataat gcgtctgaat taacccatat tccaacacca agtacaccaa
     9061 ttgaaacagc ttcaggagaa aaaattgtct ccgtcgttga tggtgtcgct tataaagaat
     9121 caggaaatgg cttgacgccc atttcagaca aggtcgaaca acttccaagc ggaaatattg
     9181 cggtgaaagg gtctgatgga aaaatgaaag tattgcctaa gactggcgaa gaagtgtctc
     9241 tcgttgcgac agcacttggt ggattattgg cttcaggaag cggttttgct ttttggcgca
     9301 aaagaaaagc aacacaaaaa atgaactaag cgaatgaagg atcaaacatg aagaaaaaac
     9361 ttcttttatg gtgtgggttc ggttgtctac ccacactatt actatgtatg tttggttttc
     9421 tgtttatgat acttttgatt agtagtggtt ctgaagagga agaaatcgac tgtagtccaa
     9481 ccgaaattgt cgcaagtact atctcattaa cgaaccaagg catggagaca aacgggcttc
     9541 aaattgcaaa ggaagtacaa aaacatattc caaatacgac attagagggt ctagcgggta
     9601 tgttaggcaa ttttcaacaa gagtcgagta tgagtcccac agcgattgaa cgaccaaacg
     9661 atccattatc tgggcatggg attgcacaat ggacggcagg gcgaacgact gctttgaaaa
     9721 attttgccaa agagcgaaac aaagagtggt cagatttagg tgtacaagtt gagtttttga
     9781 ttcatgaact aaacacaaca gaaaaagctt cacaagttgc attacaagca aaaagcgtag
     9841 aggaagcaac agaacagtgg caagtgaaat ttgagcgtgc agggatacca atgatgggga
     9901 atcgtattac ttttgcgaat caatggtacg caaaactagg aaagaatgac agtgatcccg
     9961 tcgcttcaat tacattagaa aatggggcaa acgcaggaag taaagacttg accgattatt
    10021 gtctagaaac aacaggaaca agcagttctt ccgatagtaa tgtattaaac gtagcaaaaa
    10081 gtctgattgg ttattttcat tattcacaac cgcaacgggc acaatttggt agtgtcgaaa
    10141 atccagataa aaatggctat gccgattgtt cgagctttgt gtggcttgtt ttaacgaaag
    10201 cagggtataa aacgcctgcg aacgttgggt ggtttaccgg ctcaatgacg agcgacgcaa
    10261 gaggaagtaa gcaatactta caagaaatac ctgagagcga ggcaaaagct ggcgatatta
    10321 tcattgtgaa cttaggttct ggtgtgggaa atgatggaca cacagctgtt ttagcggaag
    10381 attggcatgg actttctacg aaagtaatcg aacaaggggg aatgaatact aacggtgtag
    10441 gtgaagggca agtcaattta tcctttgggt atttgcttaa tggtggtgat atttgttttg
    10501 ctcgaccgat aaagaaataa gaggtgtcaa atgaagaaac gatttattta tgtttgtgta
    10561 ggcggattgt tcttatttgg tgtaggcagt acattttttc ttattcatga aaagctacag
    10621 ctaacagcaa aggaagagaa actgacggtg gagataaaca agttccaaga agagaagaaa
    10681 ggacaggaag accttgtaac gtcactcaaa gaacaacttc aacaagtaga aagcgaggtc
    10741 acgacgttaa aaaaagcaaa tgaagaattg aaaaacaatg attcaacaga aaaaggaaac
    10801 attcaaacct ttcaaaaaac agtgacagaa gcgttcgagg ttctgtttaa ttatgagcct
    10861 gattcttttc aagaacgaaa aaacaattgt gcaccatatc tttctgacaa actgatgaag
    10921 caatatttca aagaaggcgt ttcctatggt gattcaaacg gggtgtcttc tgaattagag
    10981 aaatgttctg tttatttaaa aagcacacaa aaagcgacta tccaagggct cgttgtggtg
    11041 tcgtatcgca ataaaattgg tgatagttcc tggaataatg ccatgaatat ttttgacgta
    11101 acctttgatc cagcgtcaaa gaaaatcacg tttatgcaaa atttgggaag tagctttaat
    11161 caacagcttt tagaagaata agaaaaggag aaggttatgg agagacaaga atcaataaga
    11221 gaggtacaag aagtaccaac agaacaagca aaagaaaccc aagaaggacg ttctttttct
    11281 tatggaaaga atttttcttt tttacgcccg tcgattcaaa tcagtgtgtt cgttttgtgg
    11341 cggatacaca ataatcggta ttcggtaggc agtcaactat tttatcaaga aatttgtcat
    11401 gctttatcaa aaaaatacag tcaaaatgcc tacaaagaag cacttgcctt ttt
//
LOCUS       DASJPC010000084        10981 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJPC010000084 DASJPC010000000
VERSION     DASJPC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.084"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            136..309
                     /locus_tag="U4A13_002204"
     CDS             136..309
                     /locus_tag="U4A13_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780477.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="U4A13_002205"
     CDS             949..1422
                     /locus_tag="U4A13_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEN1780478.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="U4A13_002206"
     CDS             1431..1658
                     /locus_tag="U4A13_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1780479.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="U4A13_002207"
     CDS             2294..2665
                     /locus_tag="U4A13_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780480.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="U4A13_002208"
     CDS             complement(2921..3163)
                     /locus_tag="U4A13_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1780481.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="U4A13_002209"
     CDS             complement(3195..4097)
                     /locus_tag="U4A13_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1780482.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="U4A13_002210"
     CDS             complement(4110..4298)
                     /locus_tag="U4A13_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1780483.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="U4A13_002211"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="U4A13_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1780484.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="U4A13_002212"
     CDS             complement(4913..5806)
                     /locus_tag="U4A13_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1780485.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="U4A13_002213"
     CDS             complement(5884..6807)
                     /locus_tag="U4A13_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN1780486.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="U4A13_002214"
     CDS             complement(6856..7206)
                     /locus_tag="U4A13_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1780487.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="U4A13_002215"
     CDS             complement(7239..8141)
                     /locus_tag="U4A13_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1780488.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="U4A13_002216"
     CDS             complement(8134..8991)
                     /locus_tag="U4A13_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN1780489.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="U4A13_002217"
     CDS             9325..10134
                     /locus_tag="U4A13_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1780490.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="U4A13_002218"
     CDS             10174..10671
                     /locus_tag="U4A13_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1780491.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3215 a         2050 c         1842 g         3874 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tattttgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 g
//
LOCUS       DASJPC010000085        10928 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJPC010000085 DASJPC010000000
VERSION     DASJPC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.085"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..287)
                     /locus_tag="U4A13_002219"
     CDS             complement(<1..287)
                     /locus_tag="U4A13_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780492.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="U4A13_002220"
     CDS             429..1547
                     /locus_tag="U4A13_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN1780493.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="U4A13_002221"
     CDS             1675..3051
                     /locus_tag="U4A13_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN1780494.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="U4A13_002222"
     CDS             3452..5032
                     /locus_tag="U4A13_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN1780495.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="U4A13_002223"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="U4A13_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="U4A13_002224"
     CDS             complement(5658..5978)
                     /locus_tag="U4A13_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN1780496.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="U4A13_002225"
     CDS             complement(6126..8360)
                     /locus_tag="U4A13_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1780497.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="U4A13_002226"
     CDS             8839..9105
                     /locus_tag="U4A13_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN1780498.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="U4A13_002227"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="U4A13_002227"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN1780499.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DASJPC010000086        10817 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJPC010000086 DASJPC010000000
VERSION     DASJPC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.086"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(25..162)
                     /locus_tag="U4A13_002228"
     CDS             complement(25..162)
                     /locus_tag="U4A13_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN1780500.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="U4A13_002229"
     CDS             complement(159..1343)
                     /locus_tag="U4A13_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN1780501.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="U4A13_002230"
     CDS             complement(1599..1853)
                     /locus_tag="U4A13_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780502.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="U4A13_002231"
     CDS             complement(1878..3020)
                     /locus_tag="U4A13_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEN1780503.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="U4A13_002232"
     CDS             complement(3080..4432)
                     /locus_tag="U4A13_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1780504.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="U4A13_002233"
     CDS             complement(4503..4847)
                     /locus_tag="U4A13_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1780505.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="U4A13_002234"
     CDS             complement(4857..5231)
                     /locus_tag="U4A13_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1780506.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="U4A13_002235"
     CDS             complement(5244..5558)
                     /locus_tag="U4A13_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1780507.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="U4A13_002236"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="U4A13_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="U4A13_002237"
     CDS             complement(8976..9638)
                     /locus_tag="U4A13_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780508.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="U4A13_002238"
     CDS             10255..10485
                     /locus_tag="U4A13_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780509.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="U4A13_002239"
     CDS             10491..10790
                     /locus_tag="U4A13_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780510.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DASJPC010000087        10784 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJPC010000087 DASJPC010000000
VERSION     DASJPC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.087"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(188..352)
                     /locus_tag="U4A13_002240"
     CDS             complement(188..352)
                     /locus_tag="U4A13_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780511.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            647..1975
                     /locus_tag="U4A13_002241"
     CDS             647..1975
                     /locus_tag="U4A13_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN1780512.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2177..3010
                     /locus_tag="U4A13_002242"
     CDS             2177..3010
                     /locus_tag="U4A13_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN1780513.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3026..3763
                     /locus_tag="U4A13_002243"
     CDS             3026..3763
                     /locus_tag="U4A13_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN1780514.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3918..4886)
                     /locus_tag="U4A13_002244"
     CDS             complement(3918..4886)
                     /locus_tag="U4A13_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN1780515.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5118..5951
                     /locus_tag="U4A13_002245"
     CDS             5118..5951
                     /locus_tag="U4A13_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN1780516.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5985..6824
                     /locus_tag="U4A13_002246"
     CDS             5985..6824
                     /locus_tag="U4A13_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN1780517.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6850..7410
                     /locus_tag="U4A13_002247"
     CDS             6850..7410
                     /locus_tag="U4A13_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1780518.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7609..9330
                     /locus_tag="U4A13_002248"
     CDS             7609..9330
                     /locus_tag="U4A13_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN1780519.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9495..10637
                     /locus_tag="U4A13_002249"
     CDS             9495..10637
                     /locus_tag="U4A13_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN1780520.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10653..>10784
                     /locus_tag="U4A13_002250"
     CDS             10653..>10784
                     /locus_tag="U4A13_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Required for the synthesis of the thiazole moiety;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN1780521.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     "
BASE COUNT         3669 a         1816 c         2233 g         3066 t
ORIGIN      
        1 gcgcatgact gttaatcatg atgtcgtagg ttcgagtcct actgccggag taaataaagc
       61 ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt attatgatga
      121 atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta gagttggcta
      181 ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc attcctacac
      241 caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta tctgtattca
      301 taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc attttattca
      361 tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa gctatcttat
      421 tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt atctcataga
      481 aatattcctt tttatctgaa aactattgta taccgtatga catttttctt catgaagttg
      541 taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt tctaaattct
      601 taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga aagctattac
      661 gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac agcaggcatt
      721 gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt cagcacatgt
      781 ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta atcaaatgca
      841 atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga tgctgaatca
      901 attccattta aaagagtttg aattatcaca aaattacact tttttctggg ataaatatga
      961 aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa tcgatagccg
     1021 gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg atatgatcgt
     1081 ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa gtgcgaatag
     1141 ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag atgcagtagt
     1201 gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa agaaagaaga
     1261 aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc caaaagaatt
     1321 cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac tgacaccaca
     1381 aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg tgatcaatgc
     1441 accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag gaaatgtcgt
     1501 tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa aattagcgat
     1561 tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc aatcttctac
     1621 tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat ttgatacgga
     1681 ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga cccatgcgat
     1741 ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag tcgaaaatag
     1801 ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct ggatggacga
     1861 atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat taaatgcgtt
     1921 caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag catagacgag
     1981 tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca gctcttttta
     2041 tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa ataaaggata
     2101 aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa taaaattggg
     2161 agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat taaatggtaa
     2221 agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa ccgaacatcg
     2281 tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag cagaagtaga
     2341 cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc taggtgaaac
     2401 aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg atcaacgaga
     2461 tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg gttttcgtaa
     2521 agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt ggtggacttt
     2581 tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga atcgatggaa
     2641 tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat cggttgaacc
     2701 aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg tgttctttga
     2761 ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc gggaacaaga
     2821 tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg cttatcaaac
     2881 tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt tatattatct
     2941 tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc aattactaga
     3001 aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc atgaagtttc
     3061 atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag gagtagttat
     3121 caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg tcaatagctt
     3181 tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc ctagagtcgg
     3241 tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct gttatcggaa
     3301 cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg ttttcgtatt
     3361 gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg ctccttttga
     3421 gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg attcgggctt
     3481 catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg ttaataatgc
     3541 gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta cgcatgttcc
     3601 agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga tcgaaagcca
     3661 ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc gaatgcaaga
     3721 acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag gttgtgacag
     3781 aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca aatttttgta
     3841 aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt ttaagtttag
     3901 ctattagata atgatgttta gttttggata aacgaaatca attcttcacc cgacaaacca
     3961 tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact gttacagaaa
     4021 ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac aggctctgcg
     4081 attccattga aacaacgaga aacaagtact acagggattt tttggacaag aagcttttcc
     4141 aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat taccaaaccg
     4201 tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc atatgcttta
     4261 acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc taaaagttcc
     4321 tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt aggtgttcgg
     4381 aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg gatctcatcg
     4441 ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac acgaatcgca
     4501 ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat tgccccagtc
     4561 aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt ttcttctaat
     4621 gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc ggacaagata
     4681 cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag attgaagata
     4741 tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa taatgggttt
     4801 gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat cgttccgccg
     4861 gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat tttgcatagc
     4921 cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga ataacgaata
     4981 gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa ccttgacctc
     5041 ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa ggaaatcaat
     5101 cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa catgacaaat
     5161 taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc ttagtggaat
     5221 tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg ataagtgaag
     5281 tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt caaggagact
     5341 ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat gttatgtcgc
     5401 caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca tcattcaaat
     5461 gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag ggttatcctg
     5521 tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa cgtacaagaa
     5581 actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag ttgctgaata
     5641 caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt cgattccatg
     5701 gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa agaacgttat
     5761 atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta gttgattcta
     5821 agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg gctgaaaatg
     5881 cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca acccaaatgg
     5941 atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc aaagctattt
     6001 tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa tcaacaaaac
     6061 aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg gggcgcagcc
     6121 cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc gtgtataatg
     6181 gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa caaaaagtat
     6241 tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt ggtgcgagaa
     6301 atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc aagcgactag
     6361 tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc caggttttta
     6421 gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa ccaatttatc
     6481 aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc tttcccgagt
     6541 gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt ggttcaaaat
     6601 tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa attatggcat
     6661 tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga gtggccatgg
     6721 ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca aatgaagaag
     6781 atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg aaagaaggaa
     6841 aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca caaaactttt
     6901 gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca tacacgtgct
     6961 gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa taaagaacaa
     7021 ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga aaaaatactt
     7081 ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact gacagatatc
     7141 ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg aatattcgaa
     7201 atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca ttatgaccct
     7261 gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc agaacctaaa
     7321 atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa atccgctgat
     7381 ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg ttatctttat
     7441 acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat agtatgctat
     7501 aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg ccaagcagaa
     7561 cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat gggaaataat
     7621 gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt tttggttagt
     7681 tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg caaggaagaa
     7741 ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca tctggttggg
     7801 caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc tacttcctcg
     7861 ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag ttttcggttc
     7921 ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat ggagaaacaa
     7981 gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg caactcacta
     8041 gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag agaagacgag
     8101 catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat cgaaaaagaa
     8161 tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg cgaggttttg
     8221 gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat tccaccgctg
     8281 tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga tacttacgag
     8341 aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga tatcgcaaaa
     8401 gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt tgaaacggtc
     8461 gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat cttagaacgg
     8521 gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga gtatatcaaa
     8581 catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc tcaaagctat
     8641 acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt ggaagaaatg
     8701 gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc ttattcagaa
     8761 gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa gcaacaagta
     8821 gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca agaaaagatc
     8881 gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca acgtttgcct
     8941 ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat tgaagaacta
     9001 agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt agttgctcta
     9061 tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga tgcagcagca
     9121 ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga agaagtgaga
     9181 gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca agaagcatta
     9241 gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg catcgaagat
     9301 ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc agcaagagca
     9361 agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga aacaagtgaa
     9421 aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag aatttcaaaa
     9481 aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc tttccgcaaa
     9541 gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca agcttgcatg
     9601 atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc gctgatttga
     9661 tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga gataactggg
     9721 tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc atttcaggag
     9781 tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga atcgaagtaa
     9841 gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa gaattaataa
     9901 gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga tcgattcagc
     9961 ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc catgtagatg
    10021 ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga gttgattttg
    10081 caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt tattggaaaa
    10141 agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat cagcgaagtg
    10201 ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta tcattagaga
    10261 taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc aatgcgttga
    10321 agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg catattttat
    10381 gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa gaaaaacaaa
    10441 tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc agtacattgc
    10501 acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc ttagatgaaa
    10561 acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt tataagaaat
    10621 tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt atggtccgtt
    10681 atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa ctagcacaaa
    10741 atgtcagaca agctttgaaa gactttccag aaatcaaaat ccat
//
LOCUS       DASJPC010000088        10723 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJPC010000088 DASJPC010000000
VERSION     DASJPC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.088"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(241..1089)
                     /locus_tag="U4A13_002251"
     CDS             complement(241..1089)
                     /locus_tag="U4A13_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1780522.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1088..2155
                     /gene="gnd"
                     /locus_tag="U4A13_002252"
     CDS             1088..2155
                     /gene="gnd"
                     /locus_tag="U4A13_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN1780523.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="U4A13_002253"
     CDS             2157..3158
                     /locus_tag="U4A13_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN1780524.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="U4A13_002254"
     CDS             3155..3439
                     /locus_tag="U4A13_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN1780525.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="U4A13_002255"
     CDS             3472..4269
                     /locus_tag="U4A13_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1780526.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="U4A13_002256"
     CDS             4283..5116
                     /locus_tag="U4A13_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1780527.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="U4A13_002257"
     CDS             5134..5991
                     /locus_tag="U4A13_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1780528.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="U4A13_002258"
     CDS             6013..6522
                     /locus_tag="U4A13_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1780529.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="U4A13_002259"
     CDS             6559..6960
                     /locus_tag="U4A13_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780530.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="U4A13_002260"
     CDS             7072..7425
                     /locus_tag="U4A13_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780531.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="U4A13_002261"
     CDS             7459..7902
                     /locus_tag="U4A13_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN1780532.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="U4A13_002262"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="U4A13_002262"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN1780533.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="U4A13_002263"
     CDS             8997..9710
                     /locus_tag="U4A13_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1780534.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="U4A13_002264"
     CDS             complement(9917..10066)
                     /locus_tag="U4A13_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780535.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="U4A13_002265"
     CDS             10462..10662
                     /locus_tag="U4A13_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1780536.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1694 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa att
//
LOCUS       DASJPC010000089        10604 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJPC010000089 DASJPC010000000
VERSION     DASJPC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.089"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(28..726)
                     /locus_tag="U4A13_002266"
     CDS             complement(28..726)
                     /locus_tag="U4A13_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN1780537.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="U4A13_002267"
     CDS             complement(719..2071)
                     /locus_tag="U4A13_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN1780538.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="U4A13_002268"
     CDS             complement(2294..3694)
                     /locus_tag="U4A13_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN1780539.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="U4A13_002269"
     CDS             3855..4592
                     /locus_tag="U4A13_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN1780540.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="U4A13_002270"
     CDS             4717..5196
                     /locus_tag="U4A13_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN1780541.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="U4A13_002271"
     CDS             5193..5960
                     /locus_tag="U4A13_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780542.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="U4A13_002272"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="U4A13_002272"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN1780543.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="U4A13_002273"
     CDS             complement(6863..7465)
                     /locus_tag="U4A13_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1780544.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="U4A13_002274"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="U4A13_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="U4A13_002275"
     CDS             8146..8730
                     /locus_tag="U4A13_002275"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1780545.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="U4A13_002276"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="U4A13_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN1780546.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..>10604
                     /gene="prmC"
                     /locus_tag="U4A13_002277"
     CDS             9799..>10604
                     /gene="prmC"
                     /locus_tag="U4A13_002277"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN1780547.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDL"
BASE COUNT         3638 a         1908 c         2088 g         2970 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttt
//
LOCUS       DASJPC010000090        10114 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJPC010000090 DASJPC010000000
VERSION     DASJPC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.090"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            119..625
                     /locus_tag="U4A13_002278"
     CDS             119..625
                     /locus_tag="U4A13_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN1780548.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="U4A13_002279"
     CDS             642..1541
                     /locus_tag="U4A13_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN1780549.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="U4A13_002280"
     CDS             1628..2512
                     /locus_tag="U4A13_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN1780550.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="U4A13_002281"
     tRNA            complement(2704..2775)
                     /locus_tag="U4A13_002281"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="U4A13_002282"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="U4A13_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN1780551.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="U4A13_002283"
     CDS             4318..5409
                     /locus_tag="U4A13_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN1780552.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="U4A13_002284"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="U4A13_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1780553.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="U4A13_002285"
     CDS             6561..7751
                     /locus_tag="U4A13_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN1780554.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="U4A13_002286"
     CDS             7855..8610
                     /locus_tag="U4A13_002286"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN1780555.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="U4A13_002287"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="U4A13_002287"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN1780556.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3541 a         1871 c         2095 g         2607 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaa
//
LOCUS       DASJPC010000091        10098 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJPC010000091 DASJPC010000000
VERSION     DASJPC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.091"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            205..684
                     /locus_tag="U4A13_002288"
     CDS             205..684
                     /locus_tag="U4A13_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780557.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="U4A13_002289"
     CDS             717..2945
                     /locus_tag="U4A13_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1780558.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="U4A13_002290"
     CDS             3074..4717
                     /locus_tag="U4A13_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN1780559.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="U4A13_002291"
     CDS             complement(4790..5335)
                     /locus_tag="U4A13_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN1780560.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="U4A13_002292"
     CDS             complement(5358..5843)
                     /locus_tag="U4A13_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN1780561.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="U4A13_002293"
     CDS             complement(5833..6645)
                     /locus_tag="U4A13_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN1780562.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="U4A13_002294"
     CDS             complement(6784..8316)
                     /locus_tag="U4A13_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN1780563.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="U4A13_002295"
     CDS             complement(8425..9456)
                     /locus_tag="U4A13_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1780564.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3372 a         1855 c         1925 g         2946 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta
     9901 atcctttttt atataagttg tttgcttatt gatggattgg ataaattaaa ccatcacaac
     9961 gagtggaaat tatatgaaat ttttcataaa aattcatata gaaacaaatg acattttatt
    10021 agaatatgac tttcattaac ataaatgttt tcaaatttca attaaattgt tttaatcaaa
    10081 ttccaatcta aatatttg
//
LOCUS       DASJPC010000092        10086 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.092, whole genome shotgun sequence.
ACCESSION   DASJPC010000092 DASJPC010000000
VERSION     DASJPC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.092"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(247..759)
                     /locus_tag="U4A13_002296"
     CDS             complement(247..759)
                     /locus_tag="U4A13_002296"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN1780565.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="U4A13_002297"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="U4A13_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN1780566.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="U4A13_002298"
     CDS             complement(3212..3655)
                     /locus_tag="U4A13_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN1780567.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="U4A13_002299"
     CDS             complement(3656..4444)
                     /locus_tag="U4A13_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1780568.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="U4A13_002300"
     CDS             complement(4441..5382)
                     /locus_tag="U4A13_002300"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN1780569.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="U4A13_002301"
     CDS             complement(5559..6146)
                     /locus_tag="U4A13_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN1780570.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="U4A13_002302"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="U4A13_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN1780571.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="U4A13_002303"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="U4A13_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN1780572.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="U4A13_002304"
     CDS             complement(9063..9998)
                     /locus_tag="U4A13_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1780573.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DASJPC010000093        10084 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJPC010000093 DASJPC010000000
VERSION     DASJPC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.093"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            162..392
                     /locus_tag="U4A13_002305"
     CDS             162..392
                     /locus_tag="U4A13_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN1780574.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="U4A13_002306"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="U4A13_002306"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN1780575.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="U4A13_002307"
     CDS             1803..1979
                     /locus_tag="U4A13_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN1780576.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="U4A13_002308"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="U4A13_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN1780577.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="U4A13_002309"
     CDS             2474..2941
                     /locus_tag="U4A13_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780578.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="U4A13_002310"
     CDS             3026..3910
                     /locus_tag="U4A13_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1780579.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="U4A13_002311"
     CDS             4024..4260
                     /locus_tag="U4A13_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN1780580.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="U4A13_002312"
     tRNA            4348..4428
                     /locus_tag="U4A13_002312"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="U4A13_002313"
     tRNA            4435..4506
                     /locus_tag="U4A13_002313"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="U4A13_002314"
     CDS             4631..4801
                     /locus_tag="U4A13_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780581.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="U4A13_002315"
     CDS             5021..5728
                     /locus_tag="U4A13_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1780582.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="U4A13_002316"
     CDS             complement(5788..6432)
                     /locus_tag="U4A13_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN1780583.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="U4A13_002317"
     CDS             6559..6846
                     /locus_tag="U4A13_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780584.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="U4A13_002318"
     CDS             6954..7274
                     /locus_tag="U4A13_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1780585.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="U4A13_002319"
     CDS             7355..8668
                     /locus_tag="U4A13_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN1780586.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="U4A13_002320"
     CDS             8665..9384
                     /locus_tag="U4A13_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN1780587.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="U4A13_002321"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="U4A13_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN1780588.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DASJPC010000094         9816 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJPC010000094 DASJPC010000000
VERSION     DASJPC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.094"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..195)
                     /locus_tag="U4A13_002322"
     CDS             complement(<1..195)
                     /locus_tag="U4A13_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780589.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="U4A13_002323"
     CDS             complement(211..825)
                     /locus_tag="U4A13_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN1780590.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="U4A13_002324"
     CDS             complement(972..1655)
                     /locus_tag="U4A13_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1780591.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="U4A13_002325"
     CDS             complement(1764..2222)
                     /locus_tag="U4A13_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN1780592.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="U4A13_002326"
     CDS             complement(2306..4069)
                     /locus_tag="U4A13_002326"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN1780593.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="U4A13_002327"
     CDS             complement(4290..5750)
                     /locus_tag="U4A13_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780594.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="U4A13_002328"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="U4A13_002328"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN1780595.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="U4A13_002329"
     CDS             complement(6907..8334)
                     /locus_tag="U4A13_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1780596.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="U4A13_002330"
     CDS             complement(8365..9579)
                     /locus_tag="U4A13_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN1780597.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2910 a         2041 c         1627 g         3238 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
     9781 ctttttttat ttttaaagat attttttcat aaggaa
//
LOCUS       DASJPC010000095         9360 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJPC010000095 DASJPC010000000
VERSION     DASJPC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.095"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(92..760)
                     /locus_tag="U4A13_002331"
     CDS             complement(92..760)
                     /locus_tag="U4A13_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1780598.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="U4A13_002332"
     CDS             893..1180
                     /locus_tag="U4A13_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780599.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="U4A13_002333"
     CDS             1596..3134
                     /locus_tag="U4A13_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN1780600.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="U4A13_002334"
     CDS             3131..4096
                     /locus_tag="U4A13_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1780601.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="U4A13_002335"
     CDS             4083..5405
                     /locus_tag="U4A13_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1780602.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="U4A13_002336"
     CDS             5646..6389
                     /locus_tag="U4A13_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1780603.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="U4A13_002337"
     CDS             6404..7396
                     /locus_tag="U4A13_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1780604.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="U4A13_002338"
     CDS             7408..8145
                     /locus_tag="U4A13_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1780605.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="U4A13_002339"
     CDS             8135..8857
                     /locus_tag="U4A13_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN1780606.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..>9360)
                     /locus_tag="U4A13_002340"
     CDS             complement(8941..>9360)
                     /locus_tag="U4A13_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1780607.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3067 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DASJPC010000096         9357 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJPC010000096 DASJPC010000000
VERSION     DASJPC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.096"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="U4A13_002341"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="U4A13_002341"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN1780608.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="U4A13_002342"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="U4A13_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN1780609.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="U4A13_002343"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="U4A13_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN1780610.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="U4A13_002344"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="U4A13_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN1780611.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="U4A13_002345"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="U4A13_002345"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN1780612.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="U4A13_002346"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="U4A13_002346"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN1780613.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="U4A13_002347"
     CDS             complement(8053..8523)
                     /locus_tag="U4A13_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN1780614.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="U4A13_002348"
     CDS             complement(8569..8994)
                     /locus_tag="U4A13_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN1780615.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="U4A13_002349"
     CDS             complement(9061..9330)
                     /locus_tag="U4A13_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN1780616.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
BASE COUNT         2355 a         2095 c         1621 g         3286 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttt
//
LOCUS       DASJPC010000097         9281 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJPC010000097 DASJPC010000000
VERSION     DASJPC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.097"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..435
                     /locus_tag="U4A13_002350"
     CDS             <1..435
                     /locus_tag="U4A13_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN1780617.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="U4A13_002351"
     tRNA            complement(759..842)
                     /locus_tag="U4A13_002351"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="U4A13_002352"
     CDS             1370..1870
                     /locus_tag="U4A13_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780618.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="U4A13_002353"
     CDS             complement(2060..2404)
                     /locus_tag="U4A13_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN1780619.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="U4A13_002354"
     CDS             complement(2558..3478)
                     /locus_tag="U4A13_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN1780620.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="U4A13_002355"
     CDS             3665..4156
                     /locus_tag="U4A13_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN1780621.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="U4A13_002356"
     CDS             complement(4180..6555)
                     /locus_tag="U4A13_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN1780622.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="U4A13_002357"
     CDS             6881..7507
                     /locus_tag="U4A13_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN1780623.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="U4A13_002358"
     CDS             7530..8234
                     /locus_tag="U4A13_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1780624.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="U4A13_002359"
     CDS             8426..9259
                     /locus_tag="U4A13_002359"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN1780625.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DASJPC010000098         9051 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJPC010000098 DASJPC010000000
VERSION     DASJPC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.098"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            197..1060
                     /locus_tag="U4A13_002360"
     CDS             197..1060
                     /locus_tag="U4A13_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN1780626.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1189..2811)
                     /locus_tag="U4A13_002361"
     CDS             complement(1189..2811)
                     /locus_tag="U4A13_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1780627.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3060..3581
                     /locus_tag="U4A13_002362"
     CDS             3060..3581
                     /locus_tag="U4A13_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1780628.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3767..5497)
                     /gene="spxB"
                     /locus_tag="U4A13_002363"
     CDS             complement(3767..5497)
                     /gene="spxB"
                     /locus_tag="U4A13_002363"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN1780629.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5662..6480
                     /locus_tag="U4A13_002364"
     CDS             5662..6480
                     /locus_tag="U4A13_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN1780630.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6583..6780)
                     /locus_tag="U4A13_002365"
     CDS             complement(6583..6780)
                     /locus_tag="U4A13_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780631.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6949..7527
                     /locus_tag="U4A13_002366"
     CDS             6949..7527
                     /locus_tag="U4A13_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN1780632.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7622..7759
                     /locus_tag="U4A13_002367"
     CDS             7622..7759
                     /locus_tag="U4A13_002367"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780633.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8572..>9051)
                     /locus_tag="U4A13_002368"
     CDS             complement(8572..>9051)
                     /locus_tag="U4A13_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN1780634.1"
                     /translation="LGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPFSTFSFENT
                     TVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLFGIGVADIT
                     DLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFLTRTVRLQ"
BASE COUNT         2831 a         1567 c         1761 g         2892 t
ORIGIN      
        1 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
       61 gaagctgacc acttctgtca caacctctta ttttgattag aacttgcgaa aataaaccgg
      121 ttaaagtgtg tctagcgcaa gagatttgct ataatagacg ataagggtaa aatgtaacga
      181 gaaggggttg aaaaatgtgt atcaaattgt aacagattct tgttgtgatc tgccatacca
      241 tgtattagca aaagaaaaag ttgatttttt atcaatgaat ctccaattga atgatcaaga
      301 gttagtagat gacctcggaa agacattcaa ttatgaccgc tacatgaatc agctgaaaaa
      361 tggtgcgatg cctacgacat cacaaatcaa tgttggccga tatttagaat ttttccgttc
      421 ttatgcggaa aaacagattc ctgttttata tgtggctttt tccagtggat taagcggctc
      481 ttacagtagt gcgcttcaag cggttgaatt gctgaaggaa gaatatgaag accctgagat
      541 ctatgttttt gatacaaaag ctgcaagtat tggggaaggg cttttagtat tggaagctgt
      601 aagattgaaa aaagaaggta aaagcatcga agaaacccta gagtggctgg aaagtaatta
      661 tttgaaagtc cattcatggg taacagtaga cgatctgaaa tatctagaac gaggcggtag
      721 gatttccaaa gcaagtgcta ctcttggcag tctattgagt gtaaagccta ttattacggt
      781 ggataaagaa ggaaggctga cgaatgttga caaagttcga ggaagaaaca agtcgatcca
      841 gacaatcgtc aatgagacgg taaaaacaat cgttgcacca cttgatcaaa tcgtttatgt
      901 ggcttacgct ggggatctag agagcgcgga gaaggcaaaa cggcttttgg aagagcagat
      961 aaagatgaaa gatgtgaagc tttatccctt aggaccaacg atcgccagtc acacaggcta
     1021 tggctgtatc gcgatttttt cgatgggaat cagtcgataa aagcagtaac tcattctgta
     1081 aaaaagccca gaaattgatt gtaacaaaaa cagcgactct taaaagatag atgagcatcc
     1141 tatctcttag aggtcgctgt ttttttattt tttaagctaa ataggccatt atgcccataa
     1201 ctcgttcaat tgtttttgaa tatctgcatt ttccaaaaat tcatcatatg tcgtatcagc
     1261 tcgatcgatc acacctttat ccgaaacggc aatgatgcgg tttgccaatg tttggataaa
     1321 ttgatggtca tgtgacgcaa ataagattga gccagtaaat gccatcaagc cgtcattcaa
     1381 tgctgtgatt gattctaagt ctaaatggtt cgttggatca tctaatacaa gtacgttagc
     1441 tttcgaaagc atcatttttg aaagcatgca acggactttc tctccaccag ataaaacatt
     1501 cactggtttc aatacttctt caccagaaaa caacattctt cctaagaagc ttcttaagaa
     1561 ggtgttatca tcttcttctt tacttgcata ttgacgcaac cagtctaaaa tcgttagatc
     1621 agtatcgaat tctttcgttg tatcctttgg taaataagaa ttacttgttg tcacacccca
     1681 acgtactgtt ccagtatctg gcgtaatttc acccatgata actttgaata gcgttgttgt
     1741 tgttatatca ttttctgcga cgaaagcgac cttgtcttct ttgctcaaat taaaagatat
     1801 atcatctaaa attttctttc catcaatcgt tacacttaca ttttctacct gcaataagtc
     1861 gtttccaatc tcacgttcag gtttgaatcc aacgaatgga taacgtctag aagaaggttg
     1921 gatatcgtct aaagtaattt tttcaagcat ttttttacgt gaagttgctt gttttgattt
     1981 tgatgcattc gcactaaagc gggcaataaa ttcttggagt tctttgattt gttcttcttt
     2041 tttggcattt gcgtttgctt gaagtttagc cgctagttga ctagattcca accaaaaatc
     2101 ataatttcct acataaagtt tgattttacc aaaatctaga tccgccatat gtgtacatac
     2161 tttgttcaaa aagtgacggt catgggatac aacgatcaca gtattttcaa aattgatcaa
     2221 aaattcttcc agccaattga tcgattggat atccagaccg tttgttggtt cgtctagcaa
     2281 aagaacatca ggtttaccaa atagtgtttg tgctaataac acttttacct tttgtccacc
     2341 agttaattca ctcatttgct gatggtgcaa ttcttcaggg atgttcagac cttgcagtaa
     2401 aacagctgct tctggttcag cttcccatcc attaagctca gcaaattctc cttctaattc
     2461 agctgctcga ataccatctt cgtcagtgaa atcctctttc atgtaaatcg catctttttc
     2521 tttcagcact tcatacagac gcttgtgtcc catgatgact gtttcaagta cagtatagtc
     2581 ttcatagtcg taatggttct gtttcaatac ggccatccgt tcatctggtc ccatagtaac
     2641 ggaacctgtg gaaggctgca gatcccctga taacacttta aggaacgttg atttacctgc
     2701 accatttgca ccaatcaagc catagcagtt tccgggggta aatttaatat tcacatcatc
     2761 aaatagtttg cggtctgaaa agtgcaaact cacatcattt acagtaatca atttttttcc
     2821 tcacttttat cattattagt tttgacaagt cttatagaat tatatagggg aaaggtaaaa
     2881 gattcaaggc aatcgaacat tctttgtgtt tcacgtgaaa cagaatgaaa aaaataaaaa
     2941 taaaagcgga gcgaaaatag ctactatagg atttattagt ttgcaacagc ataatttttg
     3001 acgttattta taagagggag tactataaaa ctgtaaaaga aatgaaagaa ggattgaaaa
     3061 tggatgaaaa aagaaccatc gactggggct cattgatttt gggaatttta tttgtacttg
     3121 tctcattgtt atcatttcaa gaccctgtcg gaaatttagt tgcaattgta gtagtctttg
     3181 cgatttttgc ctttattaaa gggatatttg aactgtttgt acggaacaga ttaaaagagc
     3241 taacaggata taaaggaaag atgcccttaa taatcgggat catcgatatt ctagttggcg
     3301 tctttttctt attcaatatt ggtgcaggag tcgcagcatt gccttttgtt tttgcagtat
     3361 ggtttattgc tgattcagtt ttagcgttat ttactgctga cttggcaaga ggagtaagtg
     3421 aaggatatta ttggtttacg atcatcgtca atattttagg aattttatta ggaattttct
     3481 tattgtttaa tccaatttct tctgcgttga cattaagctt tttagttgga ttctatttta
     3541 tgttatttgg tatcacacat atcgtctatg catttagata agtaaaatca aacaaataaa
     3601 aaagccggct tccgattaca gttttactgt tgtaattgga agccggcttt tttatcatgg
     3661 acaaatatgc acgaggtgat ttttaggtgt cgagacaaat ttatgcaaaa atgtgagaag
     3721 ctatatacac aaattttatc ttaatactcg aagcatagcg cagttgtcac aacctctttt
     3781 catataattc attcaatact ggcaaatcat ggacttcata tttttctttg aatgcttgga
     3841 tttcttcttc agaatatttt cttggatcta aatgcagatc ttctactggc aatgggcgct
     3901 tgttattgat cttcacatcg attactacag gacgatctga ttttgcagct gcatcaaagg
     3961 caaatcgaag ttgcgagtat tctgtgactg taaagctatt tgccccgagc gcttctccaa
     4021 ctttaccgaa atctgcatct tctaaaaata caccaaattt cttttgttcc gtatcttctt
     4081 gttctgcttc gatgaaacca aaagaatcat tcgaaaagac aacattaata attggaagct
     4141 gatatttgac ttgtgtaatg atatcttgca tgaccatagc aaacccgcca tcaccactta
     4201 aggtaaatac ttgtttttct gggtagctta gttgtgcggc aattcctcct ggcacaccat
     4261 tacccatagt tgcgaaccat ccagatgtcg tatgtttttg tttaccgttc atattcaata
     4321 aacgaataga atggatagtc gtgttaccga catctgtgac gaaaatcgca tcgtcttcag
     4381 caatacgatt gatttcttta tagacagcct ctggacgaat cggttcttcc tctcgatctt
     4441 caaaagattt caaccaattt acccattttt ctttattttc ttgatttgct aggtaccagc
     4501 tatcagcagg gcgagcatcc cctaactcga caagtctttg caaagctgtg tttgcatcac
     4561 cgaggatcga caaagatgtt tcatggcgtc gtccaaattt tgacgcatcg atatcgattt
     4621 ggataaattc agcagttgga ttaaaaaatg cacgtccaaa tgggaagtcg ctaccgacaa
     4681 ataagatcag atcggcttct gccaatgctt catttccggg tttagtagcg acacgtccag
     4741 caaaaccaag aaagttttca taatcatcag gaataattcc ttttgctaga acggaagcga
     4801 caacaggaac tgaaaaatgt tctgagaatt ctttgatttg cggaaatccg tttcgcgttc
     4861 cttgaccaac ataaagaact ggtttttttg ctttttcaag ataaggcaaa gcagctagca
     4921 aatctttttc ttctggtaat atcacacttg ttttgtgagt gtgagcggtg gagaaaggtt
     4981 gttcgtcaat ttcttcaaaa ccaagatcaa cagggatagt cacgatagcc acgcctttat
     5041 gctcataggc ggctttaatg gcttcatcta ctacatgcgg aagactttgt ggtgtcatga
     5101 ctgtacgatt gtatacgcta acatctgcga aaataggatt ttcatttagt tcctggaaag
     5161 agttatagtt catggatgta gaagcaactt gtcctaagaa cgctactaga ggcacatggt
     5221 ccatctgtgc atcatacaaa ccattgatca aatgtgtcgc accaggtcct gcagagccga
     5281 agacagcgcc gactttcccc gttaattttg catctgcagc ggctgcgagt gcgccgactt
     5341 cttcatgacg aacttgaatg tagttgattt cggttttttc ttgatatagc gcatccataa
     5401 ttgaattgaa agatcctccg ggaatgccgt aaatatggtc gataccccag ctttcaagaa
     5461 cttttaccat tgcgattcct gcattgattt ttgacatggt gtttcactcc tttagcaatg
     5521 atgatacata aattttttgt atctagagta tagaactttt tatcgaaaat cccaactaat
     5581 tcgtcttttc acaaaaatgt agtcatgttt ttgggaatat gctagagtag tacatagata
     5641 gattcaagag aggggaatgg catggttgaa tggctattga gacttgaacc ttggcagcaa
     5701 gcacttgctg ggacagcttt tacttatttt atgacagctc ttggtgccgg gttggttttt
     5761 ttctttaaag aaatcaaaaa agaagtgcta aacttgatgt taggatttgc ttctggtgtc
     5821 atgattgctg caagtttttg gtctttgtta gatccggcta ttacaaaagc agaagaaaat
     5881 ggagatattg cttggctagt tgtaagtatt gggtttggtt taggcggtgt gtttttatat
     5941 atggcagata aaacactgcc ccatatgcat tttggaccac aacatgaagc agaaggattg
     6001 cctacccatt taaaacggac gatcttgctt gttttttcta ttacattgca taacattcct
     6061 gaaggtttag ccgtgggggt agcattcggt gcagcagcaa ctgcggataa tccgacagcc
     6121 gctatattag ccgctgtttc ggtggcgcta ggtattggta tccaaaactt cccagaagga
     6181 gcagctgtat caatcccgtt gcgacaagaa ggtctgagtc gtaaaaaggc ctttgtttac
     6241 ggacaagctt ctggaatcgt tgaaccaatt gctggagtga ttggagcgct actagtaacg
     6301 aaagtggagc tgctgcttcc ttatgcactt gcttttgccg caggagcaat gatttatgtc
     6361 gtggtggaag aattgatccc tgaagcacag cagacattgt ccagcaaacg tcattttgca
     6421 gtttttggcg tcatgagtgg atttatcatt atgatgattt tggatgtggc attaggataa
     6481 ataaaaaaaa tatatatgat atatcaaata gggccgcgaa cttatatcgc gaccctattt
     6541 ttttagtaag ttatatcata atgaagtgaa tggtatcata cattaataat agttttttct
     6601 atgtgtattt cctttgatcc agcgaacaag ccagatgata agtgcgattg ggattagaac
     6661 tttgaccgca aaccataaaa gcccgcttag gacactgaca aaaagactga tgacgaagct
     6721 agctgcaatt agcactaaaa gaaccatgaa gatatttaag aacatatttt tttgacgcat
     6781 acaaaaccct cctcttgttc attctttact atagaaaaag tataacaggg attgtttaaa
     6841 aacacctcca actgaagact gatttttact tcatctactt aaatgatcaa acaattgctt
     6901 tttctctggt ttatcatcta aaatagagcg gaaaaggagg agaatatcat gcctaaaaat
     6961 cgtcttgaag catttacaga tgctgtaatc gcgattatca tgactatttt agttttagaa
     7021 cttcatgaac ctaacggaga cacgtgggag gcgctcgctg cattaggtta ccgatttttt
     7081 atctatattg tcagttttat cattttagcg atttattgga acaatcatca tcatttattt
     7141 caagttgtac ataagataaa tggacgggtc ttatgggcga acagtttctt tgtttttgcg
     7201 ctttcgcttt ttccgtttgc aacttcatgg atcagtgagt acatcaatag cctggctcca
     7261 gaaatgactt atggttttgt taccttgacc gctaatattg cgtattatat cttggcaaaa
     7321 gaacttgtca aagcaaataa aggccacgag aaagcattga aggcgatgtt gaataattac
     7381 aagaaatcgt atctatcgat tggtttgaat gtgttaggaa ttgtcttagg gtatttagta
     7441 gcgccagtag ccgtgctgat cgcaaacatc gtgattctag ctgcttggat catcccaagt
     7501 cgtaccatcg aatcacaata tcggtaaaat gatccgtgtt taaaatttaa gagaaaaata
     7561 tagaaaaaat aaataaacga tcaaatatcc gagtgatact agtattcaaa gagggatgca
     7621 gatggatcaa aaaagaattc atgtaggact gattactagt tttgtcacga tgcttattta
     7681 tttggatcaa ggtcatttga cttggacagt agttggtgtc gtaggatttt tgacattatt
     7741 cacagcaata aaaaaataaa catctttatg taatctgatg ttctatctat ttaagaaaag
     7801 catgtttaca gcagtatgat tctgttgtaa acgtgttttt atttgtggaa aatttaattt
     7861 ttagaaaagt gtatcgaaaa atagatagtt agtttttcca cagtggataa cttcgtaaga
     7921 gataaaaata aaaaattagt caaagaaggg cgtatagatt tcttttgata ggagatatac
     7981 aacagaaatg aataaatatt aacgtgtttt caaacagtat aaaacggtgt ggtttttgga
     8041 ataaaaaaat aacatatctc attttacgct tttgttattt tatacttaaa aaataacgac
     8101 acagaaaata ccgtgtctca gatttctcta ctacttctaa aaactactgt ttttttcctt
     8161 tgaaatgcct tcaaaaagta ggatcaggca gaatatactg gcagaatcaa ggagaaggga
     8221 gcctaaataa ggagaaaaac catagaaaat ataaagtaat tacttataaa acaagctttt
     8281 tatagaaaaa taatatacct gagtagggta taaattaaga cggaattcct ttctagattg
     8341 agatattgac taggaagaat gttaagattt taagccttta agctaaaaaa aaaccctttt
     8401 tgttaaataa ggggaatata gcctgtcagt ttcaaactgc aagtcgtcct gctgcaataa
     8461 tatagtagat tagagatgaa aatagatttt tttaaaccaa tgactgaata aaaagaccgt
     8521 catagataca cattggaatg tgtctattac ggtctttttt tacgaacgat tttattgaag
     8581 acggacagtt cgggtcaaaa ataaaaaagc tacgatcaga aataagaata aaatgtatcc
     8641 caaaataatg gctagacggt ctaacttatt tgtcgataga tgtttgttta gaagttgata
     8701 ggcccataaa cctatcagtc ctcctgttgt attagttatc aaatcagtga tgtccgctac
     8761 gccaataccg aataggaatt gaatcaattc tgccaagaag cttacaagga aaataatccc
     8821 taatttttta gaaaaagaaa gtctcttgaa attgacattc aaaagcaccc caaaaggaaa
     8881 aaagacaatc aagttataag tggtctcacg taataccgtc gtattttcaa aagaaaaagt
     8941 agaaaaaggg acgagattga cattgctgta agaataagtg agaaattgtg gaagattaaa
     9001 tgatagcttg aataaaatca accataatag aaagaataaa taaatcccta a
//
LOCUS       DASJPC010000099         8941 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJPC010000099 DASJPC010000000
VERSION     DASJPC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.099"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(342..698)
                     /locus_tag="U4A13_002369"
     CDS             complement(342..698)
                     /locus_tag="U4A13_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN1780635.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(712..1122)
                     /gene="spx"
                     /locus_tag="U4A13_002370"
     CDS             complement(712..1122)
                     /gene="spx"
                     /locus_tag="U4A13_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN1780636.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1469..3655)
                     /locus_tag="U4A13_002371"
     CDS             complement(1469..3655)
                     /locus_tag="U4A13_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1780637.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3873..4556
                     /locus_tag="U4A13_002372"
     CDS             3873..4556
                     /locus_tag="U4A13_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN1780638.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4637..5356)
                     /locus_tag="U4A13_002373"
     CDS             complement(4637..5356)
                     /locus_tag="U4A13_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN1780639.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5668..5874)
                     /locus_tag="U4A13_002374"
     CDS             complement(5668..5874)
                     /locus_tag="U4A13_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780640.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6119..6721
                     /locus_tag="U4A13_002375"
     CDS             6119..6721
                     /locus_tag="U4A13_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN1780641.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6862..8142
                     /locus_tag="U4A13_002376"
     CDS             6862..8142
                     /locus_tag="U4A13_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1780642.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8155..8883
                     /locus_tag="U4A13_002377"
     CDS             8155..8883
                     /locus_tag="U4A13_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1780643.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2598 a         1673 c         1470 g         3200 t
ORIGIN      
        1 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
       61 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      121 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      181 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      241 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      301 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      361 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      421 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      481 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      541 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      601 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      661 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      721 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      781 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
      841 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
      901 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
      961 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1021 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1081 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1141 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1201 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1261 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1321 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1381 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1441 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1501 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1561 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1621 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1681 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1741 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1801 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     1861 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     1921 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     1981 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2041 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2101 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2161 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2221 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2281 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2341 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2401 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2461 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2521 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2581 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2641 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2701 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2761 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     2821 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     2881 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     2941 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3001 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3061 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3121 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3181 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3241 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3301 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3361 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3421 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3481 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3541 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3601 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3661 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3721 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3781 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     3841 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     3901 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     3961 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4021 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4081 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4141 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4201 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4261 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4321 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4381 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4441 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4501 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4561 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4621 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4681 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4741 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4801 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     4861 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     4921 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     4981 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5041 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5101 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5161 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5221 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5281 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5341 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5401 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5461 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5521 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5581 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5641 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5701 taactgccag tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg
     5761 tatcatcaca ctattttcat agattttttc tatttttcct tttattggtg attcaaataa
     5821 atcagattta aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat
     5881 ccctccatca ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca
     5941 aacttgtcat aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg
     6001 gaaaacgaag atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga
     6061 aacaaaagac gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat
     6121 gaaagataaa gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga
     6181 agatatggtg tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa
     6241 actagcggtc gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac
     6301 ctttctttct tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt
     6361 cgtttgtctg ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct
     6421 gcaattgact atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact
     6481 gcttcgctat ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc
     6541 attattaaag aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc
     6601 agcttcttac ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct
     6661 tcctattaca aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta
     6721 aacaattaac taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg
     6781 aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa
     6841 aatcaatggg agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac
     6901 acaatttgta tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc
     6961 attctgattt cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct
     7021 ctaattgcta taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa
     7081 ttggtttacg cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc
     7141 atcgacgatt tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat
     7201 tttcacattg tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg
     7261 ttagagactt ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata
     7321 ttatttacat actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc
     7381 ttagctttga atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt
     7441 tattattgga aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg
     7501 atgcaacagg attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa
     7561 catgattacc aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa
     7621 ggattacggt cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt
     7681 ccggccaatc taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca
     7741 ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca
     7801 atcgaactgg atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac
     7861 aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag
     7921 aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac
     7981 cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg
     8041 tttatcttgg atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa
     8101 aaccagcgtt tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt
     8161 cccatttata tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaaa
     8221 aatagaatca tgattgaaaa cttagacagt tatgtccata tttctacctc tgatccggaa
     8281 gaacttctca atgcagccaa gatcagaact tcttcttttg gtatttactt tttagatatc
     8341 gaactagaag acagtgaaat ggatggtgta gcgatcggta aattgatccg tgaattagat
     8401 cctttaggaa aaataatcta tgtgacttcc catacggaaa tgtcgatgaa aatcttaaaa
     8461 agcaacatcg aaccgactga ttatatcgtc aaagaagatc tttatgattt aaaagaaaat
     8521 gtcgagcata tcctgagcaa aattttcgaa gattttcaaa tgtcccaaat ccaaaaagat
     8581 atcttcaaaa tagagtttaa tgatgagatc aaatttttac cgatcaaaga catccaatat
     8641 ttttctactt cgctaggagc gccacataaa ctggaagtcc atttgactca tgcccaatta
     8701 caattctatg agaagatcaa agaaatcgag aaaatgcatc ggaattttat ccgttgtcat
     8761 aaatcctatg ttatcaatac acaaaatgtg cggtcgatta ataaaaaaac cagagaagtc
     8821 acgttggcaa acggcgagac catcccagcc tctatccgtg gattaaagaa attgatttca
     8881 taaaaagaac cgaatagttt ggaccgacta aatgaccagc cgatccaaac ccttcggttc
     8941 t
//
LOCUS       DASJPC010000100         8798 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJPC010000100 DASJPC010000000
VERSION     DASJPC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.100"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="U4A13_002378"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="U4A13_002378"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1780644.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="U4A13_002379"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="U4A13_002379"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN1780645.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="U4A13_002380"
     CDS             complement(3070..4323)
                     /locus_tag="U4A13_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1780646.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="U4A13_002381"
     CDS             complement(4329..5252)
                     /locus_tag="U4A13_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN1780647.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="U4A13_002382"
     CDS             complement(5473..7965)
                     /locus_tag="U4A13_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1780648.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="U4A13_002383"
     CDS             complement(7972..8439)
                     /locus_tag="U4A13_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN1780649.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8575..8648)
                     /locus_tag="U4A13_002384"
     tRNA            complement(8575..8648)
                     /locus_tag="U4A13_002384"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8612..8614),aa:Arg,seq:acg)
     gene            complement(8706..8779)
                     /locus_tag="U4A13_002385"
     tRNA            complement(8706..8779)
                     /locus_tag="U4A13_002385"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8743..8745),aa:Pro,seq:tgg)
BASE COUNT         2440 a         1867 c         1461 g         3030 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc
     8581 caagaggagt cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct
     8641 atgggcgcat attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa
     8701 aaaaatcggg aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac
     8761 caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DASJPC010000101         8493 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJPC010000101 DASJPC010000000
VERSION     DASJPC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.101"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..173)
                     /locus_tag="U4A13_002386"
     CDS             complement(<1..173)
                     /locus_tag="U4A13_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780650.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U4A13_002387"
     CDS             complement(593..1714)
                     /locus_tag="U4A13_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEN1780651.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U4A13_002388"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U4A13_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEN1780652.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U4A13_002389"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U4A13_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEN1780653.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U4A13_002390"
     CDS             complement(4304..5215)
                     /locus_tag="U4A13_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780654.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U4A13_002391"
     CDS             complement(5232..6239)
                     /locus_tag="U4A13_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN1780655.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U4A13_002392"
     CDS             complement(6236..8365)
                     /locus_tag="U4A13_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN1780656.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8493)
                     /locus_tag="U4A13_002393"
     CDS             complement(8370..>8493)
                     /locus_tag="U4A13_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1780657.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DASJPC010000102         8274 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJPC010000102 DASJPC010000000
VERSION     DASJPC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.102"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            296..829
                     /locus_tag="U4A13_002394"
     CDS             296..829
                     /locus_tag="U4A13_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780658.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(955..1632)
                     /gene="nth"
                     /locus_tag="U4A13_002395"
     CDS             complement(955..1632)
                     /gene="nth"
                     /locus_tag="U4A13_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEN1780659.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1639..2337)
                     /locus_tag="U4A13_002396"
     CDS             complement(1639..2337)
                     /locus_tag="U4A13_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN1780660.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2605..3789
                     /locus_tag="U4A13_002397"
     CDS             2605..3789
                     /locus_tag="U4A13_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN1780661.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3795..4238)
                     /locus_tag="U4A13_002398"
     CDS             complement(3795..4238)
                     /locus_tag="U4A13_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1780662.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4464..4751)
                     /locus_tag="U4A13_002399"
     CDS             complement(4464..4751)
                     /locus_tag="U4A13_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780663.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4922..6274)
                     /locus_tag="U4A13_002400"
     CDS             complement(4922..6274)
                     /locus_tag="U4A13_002400"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN1780664.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6547..6993)
                     /locus_tag="U4A13_002401"
     CDS             complement(6547..6993)
                     /locus_tag="U4A13_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1780665.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="U4A13_002402"
     CDS             complement(7229..8155)
                     /gene="cysK"
                     /locus_tag="U4A13_002402"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN1780666.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2277 a         1656 c         1320 g         3021 t
ORIGIN      
        1 aaaaaagaaa ccacgtctac aagcgtggtt tcttttttgt gtctatgtaa aaaacagcta
       61 caaataaaaa tcatgtacca agctgtatta catgattttt tataagacta tccagcatat
      121 acatgtgtga tctaacattt agcttaaaaa ttagaaaatc catttttctt ttatcattac
      181 atatacttta tccatttact tgtttataat taaatcggct aaatacacac tagtctttta
      241 tttagtgcca cttcttaata atcccattgt tattatcaaa taaaaaatac aatatatggt
      301 tttgaataat tttttgaaag aaggcttcat tttgagttat ctgatagtat gcttaattat
      361 cataatttgt ctgcaccatc tttttttatc gaaaacaata cctcgttatt ccaagaataa
      421 aaaagctgaa aaattttgtt tattattaaa taaatttact ttaattgctc ctatccttgc
      481 atttattatt ttttctgtac ttttaagcac tactcttaaa ggaaaattta tggaaagaag
      541 ctctcatgca atgattctca cttttttatg gttgttgttc accagaatct atatttttct
      601 gatgagcctg aaaccgccca aatccattag tttatgttta gttataaatg gtattttttt
      661 attatcactt atcattttta ttacaccttt agatagatac gtcacttatt tatataatcc
      721 tttggaatac tggacttatt ttataggtat attggaaggc attattttct acatcgggta
      781 ttttcccaat aaaaataaca acttttattt ttatcgcaat aaattgtaat aagttaccat
      841 cctagctact gagtatgtac atttactttt tcatgcaaaa aagataaacg aaacagcttt
      901 tctccataat aacaagataa ttcatacatt tatttgcaat taaagaaacc acgtctacag
      961 gcgtggtttc ttttttagcg ccttttcttt tagacgcatc cgatttttcc catcttgaca
     1021 tattgatagc aaaggacata cctcgcactt gggatttctt gccgtgcaat gatatctacc
     1081 aaaaaaaatc agtgtatgat gtgtctttac ccataattct tgtggtactt tccgcatcag
     1141 tgtttcttct acttccatga ccgtagcatc tagtttgcag atcctcaggc gttttgagac
     1201 acgttccacg tgggtatcta ctgcgattgc tggtatcccg aatgcatccc ccaatacgac
     1261 atttgctgtc tttctgccta caccaggaag agacatcaat tcttctcttg acgttggcac
     1321 ttgcccatcg aaacgttcaa tcagctgctg tgcacacgct ttgatgtttt ttgctttatt
     1381 tcgatacaaa ccaatcgttt ttattttaag aatgatttca tcgattgaag cttctgctaa
     1441 cgcatcgggt gtcggaaaag aggcaaacaa atctggcgtt gctttattca ctgatacatc
     1501 tgtcgcctgg gcacttaaga tcactgcaat caataactca aatggattat tgtgcttgag
     1561 ttctccatgt gcttcgggaa acattccata catcgtttct agagcttcca ttgttttttg
     1621 tttgcttaac atattccgct actcctccaa ccaatttttc agtgagactt tcggcaattc
     1681 ttcttgttga gctgactgtt ctacttctct ttgaagaagc tgctgttttc ttcgtttttg
     1741 gtcttcttcc acttgttgtt ttgtacgtag attttttctt tcccacgata gcaaaatgcg
     1801 gtcaacgtaa ttaaaactat aagcctgatt taatacggct tcccgcagtg ctagcttcaa
     1861 aatttccggc tgataatggt cttcttctag ccattgtccg atccgctgga actcgatcga
     1921 agaaagcggg cgtccaaatt cttgttcaaa taattgatac actgactgga tttctttttc
     1981 ttgtgttttt tcttcgtatt ttttttcttg tgtttttaaa tactcaccta atagatcata
     2041 gatggggtaa agattatatc gatctgcttt tttaccgttg acaatcgtcg tttctatctt
     2101 gataaatcca tttgttacga gacgatccag tatttgaaaa atctgatctt gtttgattcc
     2161 catatccata gcaatcatct gcaaatcagg gaaagtatct ccttcaagct gtgccatatg
     2221 caactgtaga acaaataaaa attcttcaga ttgtagaccg atcctatgat aatttttaag
     2281 taaaagatta gatatgactg tttgtccagc gcttaagtaa ttttctaaat tcaacatgat
     2341 ttctcctccg ctcaatcaca gtatacgtaa aaaaaacatg ggaagcaaga acaggtttct
     2401 tttctattta cttcactgat cggtcagatt tacaatgtcg tctacttgat ttttgtttgt
     2461 tcatgttttt gatgcgtatc ttcacttttc ggaataaata aattaagtag atttcgctgt
     2521 tccttagaat gttgacaagg aaacaaaatt actatacact atttttgttt ccaagttaac
     2581 attattgcat aaaggagttt ttacatgtac agtttcacaa ataaacattc taaagttttt
     2641 ggatacgtct ctgtcttatt cactggatta ggatttacta tcgtcagtcc tgtacttccg
     2701 tttttaacgc tgccttattc tcactccata catcagcaag ctttctatat cactttgctc
     2761 atgtctgttt atgctctagc tgccttctta tctgccccca ttctcggtag cttaagtgat
     2821 cattttgggc gtcatccgat ccttattatc agtttattag gatcaagtat cggatatttg
     2881 ctttttggtt taggaaattc gatatggatg ttgttccttg gtcgtatcat tgaaggattg
     2941 acggcaggag aaatctccac cttatatgct tactttgccg acatcacgga acctaatgaa
     3001 agaaccaaag tttttggatg gatgggtgct ttagtcggta taggaacaac gctgggtcct
     3061 attatagggg gtctattagc tgagttgggg aattctgttc ctatatttat tggagcactc
     3121 tttactttct taaatgccgt ttatggctac acatttatgc tagaaagcct tccaataaaa
     3181 aaacgttcag tagatttaag tttctcccat gtaagacctt ttcaccaatt acaacaatta
     3241 ttcaagattt catccgttat tcctttattg acagcaggtt ttgctgtttg gttggcagct
     3301 ggctctcttc aatctatctt ttctcaattt tcgattgacg cctttcagtg gaaggctggg
     3361 ttggttggtt taagcttctc tttgattgga atcttggata ctgtctcgca attgttcatt
     3421 atgcctcgat tgctcaaaaa attttcagaa cagcagatca caagaatagg gatgttcagt
     3481 gaaatcctcg cctatctatt catcacatta tctggaattc ttctactgcc gattttgttt
     3541 ttatttggga ttatctgtta tggtttcggt gattctattt ttactcctgt attcaatggc
     3601 caactatcca attctgtctc agataaccag caaggtcttg taatgggcgg tacccaaagt
     3661 atacaatctc tatcgcgcgt catcggcccg ctaatcgcag gacagcttta tgctgcagca
     3721 ccatttcttc cgacggtatt cggatttttg cttatgattt ttgcctatta tcactacaat
     3781 aaaaaatagt agcttcattt gctacttcgt ataaattctc tgtatcgacc gataaaacga
     3841 aatactgtgt caaattcttc ttttgacata ttttcaaata cagtagcatc tcgctgtacc
     3901 catttttgat ggagttgctc gtgtctattc cttatttttt gtccgattgg cgtcaattga
     3961 aaatagattt cttttttatt ctcttcgctt tgataagtgt ttatgatccc tctagctatc
     4021 agtttttttg ttagcttact gatggctcct ctagtcatgt aactagcatt cgctaatttt
     4081 gtcacgttag gatcagggat tttcccgata tgttcaatca cttctagttc atttagagta
     4141 aatcctgcaa attctttttc catttctggt ttattacgat gaataatctc attagctaaa
     4201 tccagatatt cttctattag cagttgtctt ttatccattt ctacaccttc tttttctttc
     4261 attatattgt ttttttgttt cctattcaac aaagataaaa atattttgta ggaaaaccca
     4321 atctgaaacc aagaaaaaaa ggggttgaca aagcttgtca taaccccttt ttctgattaa
     4381 aagatagtaa agagacagtc atcggtaatt gtgatgattt tttgtcatca cttaaggatg
     4441 agcggccttt tcaaaaactg tctttaaaga tcatcatcag tttctagcac atatggctga
     4501 ccttcatatg tgtactctgt attcaatgtg ccgtcatttt catctgagta gatgcgtcta
     4561 ctgaaaggaa aacgatgaga aacattatct ttggcgtcat ttgctggcat cgtgtaatat
     4621 tcctcttgcg tttcattcaa ctgagagagt acttgatctg ccaactcatc aatattgatc
     4681 gttaatttct tcgaagcact tttataatct tctaaaaatg catcttgttg gacaaaatat
     4741 tctttattca tcttctttct cacctttctt ttcaaaatca aataaagctg cttgttcttt
     4801 tatgtaacag ctttatcttt gctgcttatt gtgacaaaaa attcgaagaa agacaactaa
     4861 ttgtactttt cacaaaaaaa gacagagggt cgtgacaaaa ttgtgtcaca accctttttc
     4921 tttattgtgc agtttcgatt tcgcctagcg ctgcttgtgc aagtaggttc aagtaattcc
     4981 atgggcggtc aaaatgtggt tggaagaaga aatctgaaag tgcgagatct tctacagtca
     5041 ttttattttg aacagctaaa gacatggtat ttgctgattg tgtaatgtcg tatttggaca
     5101 tgaactgagc acctacgata cgattcgttc ctttttcata gactagctcc atcattactt
     5161 tttcagtagt tggcatgaac tctggacgat aattatcttc aaatacagtc gcttggactt
     5221 ctagatcgtt caaagttgcg ctttctttgg tcacacctgt cgaaccgatc gtccagccaa
     5281 ataggtaaag tcctgaagtt ccttgagtac cacgatacgc cattttatga tctgtcaagt
     5341 tatttcccac aagcaaacct tgacgaacgg catttgttgc caaaggaata tagttttgcg
     5401 tgccacttgg attgtagttg actactgcag agtctccagc tgcaaaaatg tctggattac
     5461 ttgtctgcat gtattcattc actttgattg ccccgtttgg cagtgtatct acttttcctt
     5521 cgatcagttt tgtatttgga cggaagccca cacataaaat gaccatatct gtatcaaatg
     5581 tatctgttgg tgtagctact tgtttcactt ttccttcttc atctgtgata aattcagtaa
     5641 cattttcgcc tagcgcaaga gtaacaccgc ggtctgtcaa ttctttttct aagatatctg
     5701 tgaatggttt atctagatat ttattcaaga tgcgatctaa tccatcaatc aatgtcactt
     5761 gttttccaga ttcgacaaaa gcttctacta attcaatacc gatatatcca ccaccaacaa
     5821 ttactacacg ttttgcttct ttcgcttgtg caataatttc atttgcttga ttgtagtttt
     5881 tgcataggag tacatttttc gcatcgattc cactaatcgg tgggataata ggccatgaac
     5941 cagttgtcat cactaattta tcataagaaa ccgtttctgt ttctcctgtt ttcagatctt
     6001 ttgccgttac tgttttattt tctgtatcga cgtctgttac atcgtgttcc attttaactg
     6061 ttgctcctaa tgaagataat tcttcaggat ttgagtagaa caagcctgct gggtccttta
     6121 ctactccgcc gacataaagt gcgataccgc aagataagaa agaaatattg tcatttcttt
     6181 catatactgt cacttctgct tctggatgat tttttaaaat actttttaca gctgcagtac
     6241 ctgcatgagt acagccaatt actactactt tcatttatgg tccctccgat taacattttg
     6301 tccttcgaca aaactactat aacagaaata aaacggatac aacagtttaa tgcttgtgaa
     6361 ttcacaaagg tttgtttgta gtttcattcc ttttcaagca taagaaccca atatctacaa
     6421 gattcttatt ttaataatat tcacttttat atctaagatt gtgaataaaa gacaaaaaaa
     6481 taaaaaagct agcgtttttt ttacacagct agcttttttt aggtataaca cttgtagaaa
     6541 ctgtttttac atttgcgcct tttttgctga ctctttacaa tccggacaga gtccatacaa
     6601 ttccaaccga tgttcattaa tctcaaaatc agtcagtctg cttgcagcca tttctatatc
     6661 ttcaagccct gggtaatgaa aatcaacaat cttaccgcag ttttcacaaa ttacatgata
     6721 atgacgcttt gaactgaaat caaatcggct agaagcatcg ccgtacttca tttcttgaac
     6781 aaatccaatc gaagtaaata agcgaaggtt attgtaaaca gtcgcaacac tcatatttgg
     6841 aaaccgatgt tcaagtgctt tataaatctc atcagccgtt ggatgggaat gatgttcaat
     6901 caaatattct aaaaccgcgt accgttgagg tgtgattcga atgttctctt tttttaactt
     6961 ttcgataacc tcttcgacta acttattttc catgtaaaca ccccacgttg ttattctcgt
     7021 cttatcatac tatttttaca ttttaaaatc cacttaacat gaaaagaaaa ttcaatttta
     7081 ttgtatttta atgaaaaaag acgtaaaaca gtcgcgttca ctattctacg tctctattca
     7141 aataattttt ctcatcataa atttttgtgt gttcttttca tctttaaaag ttttttacat
     7201 aaacctatct ggagaaaaac caagtgcctt atatctcttg gtaaagggct gtagataaat
     7261 aacgttcacc attgtcggga accacagcta ataccttctt gccttttcct aattcttttg
     7321 cgacttttag cgcggcagca attgcagcgc cagatgagat gcctactaaa atcccttctt
     7381 ttcgcccaac ttctctagct gtttccatcg cttcgtctcc actaatagaa aggactttat
     7441 catacacaga agtatccagt gttttaggaa caaaacctgt accgattcct tggattttgt
     7501 gaggtcccgc ttctttccct tctaaaatag cagattctgc tggttctacc ccgattaatt
     7561 cgattttcgg atacacacgt tttaattctc ggccagcgcc agtgatcgtt cctcctgtac
     7621 cgatacctgc aacaaacgcg tccaagccat ttactccaaa tgcttgatgt atctcaggac
     7681 cagtcgtctt ctcatgcaca agaggatttg cttcattttc aaattgtaat ggtaaaaagt
     7741 agccgttttc ttttgccaaa cgttctgctt ctctgattga tccagaaata ccatccgcac
     7801 caggagtcaa aatcaactct gcgccgtacc ctttcatcaa taaacgtctt tcgatactca
     7861 tcgtctcagg catcacgatg attactttat atccttttgc aactcctacc atcgacaatc
     7921 cgattcctgt atttccagaa gtaggttcaa tgatagtatc acctggtttc agtagaccat
     7981 cgtgttctgc tttttctatc atacttaaag caatacgatc ttttacactt cctcctggat
     8041 taaaaaactc aagctttaca aaaacatctg ctgaatcttc tggtacgatt ttatttaatt
     8101 tgacgattgg cgttttgcca atcaattcgg tgactgagtt atagatagct gtcatcttca
     8161 attcctcctt gtctttgctc ttcttactta tcataataca atctgttcat ttatggcgag
     8221 tgattcgtct ttgactagca aaaaaaagac aaagatatac catatatggt aatt
//
LOCUS       DASJPC010000103         7914 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJPC010000103 DASJPC010000000
VERSION     DASJPC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.103"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..199)
                     /locus_tag="U4A13_002403"
     CDS             complement(<1..199)
                     /locus_tag="U4A13_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780667.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
     gene            complement(362..1996)
                     /locus_tag="U4A13_002404"
     CDS             complement(362..1996)
                     /locus_tag="U4A13_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1780668.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2000..3373)
                     /locus_tag="U4A13_002405"
     CDS             complement(2000..3373)
                     /locus_tag="U4A13_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1780669.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3389..3628)
                     /locus_tag="U4A13_002406"
     CDS             complement(3389..3628)
                     /locus_tag="U4A13_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780670.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3810..6485)
                     /locus_tag="U4A13_002407"
     CDS             complement(3810..6485)
                     /locus_tag="U4A13_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEN1780671.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6668..7591)
                     /locus_tag="U4A13_002408"
     CDS             complement(6668..7591)
                     /locus_tag="U4A13_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780672.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2320 a         1560 c         1198 g         2836 t
ORIGIN      
        1 tataataaac atttccttga ggtttccttt taatcccctt cggaaaatca ccttttgtaa
       61 aatcgctctt aaggaaatca tctaaattac cagttctggt ctttttacct ccagtattcc
      121 ctgatttttt aggtggtaca tgttctttag taagttcagt gtatttttca cataaaatac
      181 acttacccat ctttttcata tctatcccca ctctttttaa ttcttatata caattatatt
      241 tcaaatattc attaataaat atccctattt atgataagga cctccctaac tacttctcta
      301 acgatttcaa atcgctagag aagcgttagc gcccctctaa cgttgtttga tataacctag
      361 actagatcgt taacgtaaag ggtgttttta acacaaaaat agccgtatcc tttgttttgg
      421 atacggctat ttgatattgc tcattcaatt gttgtaccac ggcatgcgtg aatttcatcg
      481 taatttccaa catctcctta tggttaggat caaaggttat tttctctata aaggtacgat
      541 acaaggcttt gatttgcttc ttctcactct gggcaagaaa acgatccaaa ttagtcaata
      601 tccgttggac atctttgact tgaatcggct ctccttgttg aaagatccct aagatttcat
      661 tttctcgaat gtgcaactgt cgtcgttttt cagtcaattc gtctaagcgg tcttttaaca
      721 tcggaaacaa ttcaggacta tcttccaaag cagcttccca tttttcaatt ttagtcttca
      781 attcttcctt tcgttccagt aacaccacga gttcttgttc caatggttgg ctttgtcgca
      841 ctatttcttc atttaacgct gccaccaatc ggggaagaac ttctggtagc tgaacgatct
      901 cctttaatcg gtcggcgaca aatgtttccg cttgttcggc acgaatcgaa ttggcatgac
      961 acacacttgc tcctttattc cgaaagactg aacaagaata atatcgaatc cgtttctttg
     1021 tgccatcctt tagggtattt gtgacattac tcgctgccat cggtgcgcca cattctggac
     1081 aacgtaacaa gcctgtcaat acattttcac ctgtatggtt ccatttgggt tgttttcctt
     1141 ctaaagcaag gcgttcttgt actttttgat aatcttcttc cgaaataatc ggttcatggg
     1201 ttcctttcac taaaatcggt ctcggattct tccccttccg tcgtttggtt tcccaattga
     1261 tgtagcgcgc atattctaaa taccctgcat aaattttatt gcgtaagata tctttgactg
     1321 ccgtggtaga aaaagcattc tttttgactg tttgatatcc ttgacgattc aaaaaattgg
     1381 caatcgctcg ataccctgct ccgcctaaat attttgcata gatcagacga acaattttcg
     1441 cctcttctgg ttcaatgacc aattgttttt gaccattcgt gtcaatacca ctgacatagc
     1501 ctaaacgtcg gcctcctgca taacgctttt tatctttcac taagctttgc attgacattt
     1561 gaacattctc cgcaatttgg ttgcgttcaa attcaccgaa actcccaagg agttgtaaca
     1621 tcaatttacc tgtcgaagtg gtcacgtcaa attgttccga gatcgaatgt aatcctacgt
     1681 catatcgcaa taattcctcg actgtttgta agacatctaa catattgcgt ccgagacggg
     1741 tcaatttcca aatcactagt agatcaaaac gatggttccg agcatcgttc atcattcgtt
     1801 gataggcgaa ccgatttttt gtggatttcc cactgattcc ttcatcggca taaacttcta
     1861 ccacttcata gcccatccgt tcggcaaact gtcgtccatt ttgttcttgt gcttgaatac
     1921 tatgtccttc taaagcttgt tcttgtgtac tcacgcgaat atacaatgcg actcgtttgg
     1981 tttgtatcat ttccatagat cattcgttcc tttctaaaat attttgtgtc agttgtcgga
     2041 agtaaatccc acaaactgtt ttatcctttg ttacctcaat ccgacaaaca tagtcacata
     2101 ataattgtcg actagagagg tcttttagtt gaagccattc tttcactttt gcttcacact
     2161 gttggagtga aagaaacgaa gaagtagaca tcggcttttg aagagcttgc agttgttgct
     2221 tgagttcttc cagtgaacaa ttgccctctt caaatgcctt aatccattgt gcttcttgtt
     2281 tcttttgttg gattactttg cgtttttcat cttttcgctt ttcggctaat tgttgcgtta
     2341 attgggcaat caaggcttga tagacgcctt cgtattgaaa aaaagcctgt atcttttcca
     2401 atacttctga ctcaaccctt tctgcccgaa gaaaaattcc ttggcagctt cggtcggtac
     2461 aatgataata atgaagcgtc ttcgtcttct ttgtattcca ttgatacttc gaactcagat
     2521 gacgaccaca gaagggacac cgaatttttt gacgtaataa gtacagatac gtatcttttt
     2581 tagctgtaca ccttgattgg cgaatctctt gggcttgtaa aaaggttgct ttcgagagaa
     2641 tcggttcaaa ggttcctaga tacttcccca aagatcctcc tttgatttcg ccatagtaca
     2701 atggattttt taggatacta taaatatgat agggctgaaa aggacgttcg cggaagaaaa
     2761 gcttttgttg cgtcaatcgt tgacaaagct ttttgtagcc atattttttg gtcaaataaa
     2821 gttgaaaaat atagcgaaca atccttgctt cgtgtacttc ttgttggatt ttcccattca
     2881 ctaaacgata cccatatggt accgcagaag acaaatattg ccgttgttgc tttttatagg
     2941 aaagtccggc acgaacattc tcaataatga cgtttcgttg ttgttctgca aaagtgaaca
     3001 ataattgcac aaatagttta ttcgttgacg cgtgtgagaa agaagcagga agaggttcag
     3061 aaatcgaata cacttgaacc tgatgttgtt ggcacaactc aataaattct aaggctattt
     3121 tggcttgacg tgccaatcga tcataacgcc aaacatagat caccccctct tcttctgacg
     3181 cacaaaccca agctctaagc tgtcgaaaag ctggacgctc cgctttttcc cctgattctc
     3241 ctttgtctga aaatcgttgg ggaatttgtt catatttctc taaaacatac tctttcactg
     3301 catcatattg agcgtcatta ctaattttcg cttcttcata aacagacgtt cgatcataac
     3361 aagccacacg catgcttgat tcccctctct atgaatgaac taaataccat tttttcacga
     3421 ctcttctatt gaacgaacgt tgcacaaagt gattttctaa atatccttcg ttcttcaacg
     3481 cttgaagagt acccccgata gatccagcag atacccctct tatttcgaaa tccaagtaaa
     3541 ttggttttgt aaaaaaggct tgatttgaaa aatgttcttg aatatacaga agaacctctg
     3601 cttttagtgt cattttattt ttagtcatta gaattttcct ttctttcatg tttgattgaa
     3661 tgaagtaaat gaagcataga tcaagcttca tttacttccg tttgggtttg ctcgctatca
     3721 tacagataag taatcagttg ttttgctaaa tcaataagaa atatttccat aacatggtgc
     3781 ctcactttca tactttggtt tattctaaat tacaatcgaa aatacgattt gagtgaagaa
     3841 ggtaatagaa tctcgtatgt tggaagaact tgacgattca tgtgaatacg tttcgttctt
     3901 ctcccttttt ctgcacacat attacccgaa tcaagcaatt gcctaaaaag ataggttaga
     3961 tcctttactt gaaattctcg ccttaacaag tcctcaaacg ggataggaaa aatatttacc
     4021 ttaatatccc catttttttg aattgatact tttccccaaa tcgtactggg cgtatgtttg
     4081 ttgtcactgt ttgttaaaaa tttatggcta ttcatcacca acaatgtttt cactcggtca
     4141 aataccgttt gctcacttcc taaactagta tctacaatta actgttcgta cgaattaata
     4201 agcaccgtct gtatatgctc taatcgtaaa ggatagccca gtatatcttg aattaggagg
     4261 gctccactat aaatcaaagc tagattatta ccaatgcgtt gcttcaaaga atgttcaggt
     4321 aaaacagccc atagtttttt ttgagcttcg acaaaagttg cttcaatttt tgtaatccct
     4381 tgttcaaaga gtttcaaaag aaataacttt ataccccaac caaaagtatt agaagaaaga
     4441 cttttaactt tctctgcgtg attcgcatcc tttgtaaact tgatatcaga aaattcgata
     4501 tatcgaactt tcaaaccagt taatttctca ttgaaataat tttccaaacg ctcttcagaa
     4561 gaggtaaggg tcaaaacaga aaaagtctct tgtggcataa gctgacaatt agaatctaat
     4621 cttagccttt cttctcctga agctaattga tagatcaacg agctaaagtt attttgagtt
     4681 gctgctccta actcatctag tattagtgga acacctccct gattgtttga tgctagcttt
     4741 ataatggctt tattggtagc attcatattt ctaagaaccg tagttggatt tccaaataaa
     4801 gaggcaataa agtgtaacgt agtggtttta cccgttgtgc tcttgccctg taggttaatt
     4861 agtaaactca aaaaagtatt agccttcgta acgtttaaat agcctaacac aatagaagca
     4921 ataccaatcg accaagcaag ttctaagttc gttcctaaaa catacttatg aaccaatgcg
     4981 tcaaattccg cttctgtacc attagttgtt aattggtagc atgagttttt agttaataca
     5041 gaaggaattt cacgtccaaa agaatcttgt aactgaaaaa tcattgcttc attttctttt
     5101 ttccagccaa gagattcatg ttcaaaaaag attggagcat tctcccgttg aatctgtaaa
     5161 aagtcaacga cttcagcttc tcttctagct ttaatgatta agccatgttt tgctaattgt
     5221 ttaaaattat tagtttgtaa acatccacct tctatttcta tttcatccca acaatgatcg
     5281 acataatagg caagaacaag tgtaatgact ttttgttcga tatttttgcg tatttgctta
     5341 ataaacatcg gacaagcaac tttttcttct tgaccttctt tcaccaaaaa tactccttct
     5401 tgattcagtt taaactggtc aaaataatat tcttgattgg attctaatga ttctacagaa
     5461 ggatttacag gttttggcaa cggatatttt tttacaaagt cttgaatatc tttattttta
     5521 tccgaagcat gcagacaatg aaatcgaatc ttattatcag ccgtaacaga caaacaagaa
     5581 ccattagaat tattcgtgtg ttttcttaat ggacattcgt caaatataag taaggtaact
     5641 cctttatgat cgccctcttt tatggtataa gttaattcag gatagtgagc taaccattgt
     5701 ttatagtctg cttgaataaa aggtaacact tctctaaaag atggatggct ttttactcta
     5761 gaggttgaca tcatggaatt ttcttcaact atctgttcaa gaattttaaa acaattactt
     5821 ctatctatgt tatcgggaat agaaaggaaa ctagataaac gatgcggacg ctcttttgtg
     5881 gactttcctt tgaccgctgg tgtaccaact aatttgcata atcttgaagg attataaaca
     5941 gaagtatcaa tatcaacttc atcttctact ccaaatcttc ggtgaagtaa ttgaagaaaa
     6001 ttctgaatag taagaatcgt ctcgtgatta tttggttgtt catttatggg aagaaaaata
     6061 tggtagccat tcccactatc aacttttaca aatgattcta ttctcttttc tttcaaataa
     6121 cgttctactt gttccataac attttctgcc tttttctttt cgctatctgt tgcacttccc
     6181 tctttccttt tagggtcaat atcaataaat acaaaccgaa ttttttcaat atcattgtct
     6241 ctcacagaat gctttttttg tgtaatcacc tcttgtttaa taggattata agttatgttt
     6301 actgtacctt cagacaattt ctgtttttct aaatagttaa taattgcttg agaatctgca
     6361 gtagaaaaaa tatgagaaac ggttttcttg tttaatgtag aaaaagttct caaattaaca
     6421 taagaaatcc ccagtgtatt taacagttgt aaatgcttct gaattgaagt gtgattcatt
     6481 ttcattaatt accactcact ttctaaaaaa tttttatttg tatgactaca ttacctccaa
     6541 cctctcataa tttcatgatc ttcagttcta caaaaatata tcccactcaa aatcttgaca
     6601 aaagaaaaaa gttgtgattt tggaaatcac aacttttttc ttttacgata aatcaatcct
     6661 tattaaatta tttaggaaaa tattgttcaa ttatttcttt atatattttc tcatagtatg
     6721 tttctgtcca tcctaatgtt cctataaaat caagtcctct acattttaaa ggttcactta
     6781 aactattaat atagttaata atgttgtcta tccttttctc tttatcttca ccttcaatct
     6841 ggggagcata tagtccaatt tcctcccaat aatatgaatc tctttcaatt cccatgatgg
     6901 tagctttggc taatttcaaa tcaaataacg attttagaat tcttagtgtc tcaataaagt
     6961 cttctctctt caactcttta atccattgtg aattttctgg attatctaaa atttcaagtt
     7021 catcttcatt ataagtatat ttcaaatttg acttgcttaa ctcatagata aatgaagttt
     7081 cttgactcat aattctttta agtacttgac tataagtttc gtttgacttt gcccatttcg
     7141 ctaattctac cactgtattc actaataaat cgaaataatg atgttctcgt tcaatagtct
     7201 cagcattttg aaaatgaaaa tcacctttat aaactccatt cttttccact gttttattaa
     7261 tattttttaa aaaccagaat gagtttgcaa atttagtcgt actttttcga ttaactaagt
     7321 taataactct tccatgtaat aataagacaa aaatacataa atttttagct tccttattat
     7381 tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat tgattgctca
     7441 taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac tcataattcc
     7501 catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta tccacatcaa
     7561 aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca taataaattt
     7621 catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa agatacgtca
     7681 attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg attgagtccg
     7741 cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac agggagagaa
     7801 agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt tcctaccgct
     7861 aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc tttg
//
LOCUS       DASJPC010000104         7877 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJPC010000104 DASJPC010000000
VERSION     DASJPC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.104"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..995)
                     /locus_tag="U4A13_002409"
     CDS             complement(21..995)
                     /locus_tag="U4A13_002409"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN1780673.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="U4A13_002410"
     CDS             complement(1075..1950)
                     /locus_tag="U4A13_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN1780674.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="U4A13_002411"
     CDS             2080..3987
                     /locus_tag="U4A13_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN1780675.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="U4A13_002412"
     CDS             complement(4528..7806)
                     /locus_tag="U4A13_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN1780676.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2221 a         1664 c         1463 g         2529 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaata
//
LOCUS       DASJPC010000105         7859 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.105, whole genome shotgun sequence.
ACCESSION   DASJPC010000105 DASJPC010000000
VERSION     DASJPC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.105"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            278..787
                     /locus_tag="U4A13_002413"
     CDS             278..787
                     /locus_tag="U4A13_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN1780677.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U4A13_002414"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U4A13_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1780678.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U4A13_002415"
     CDS             2322..3302
                     /locus_tag="U4A13_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1780679.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U4A13_002416"
     CDS             3424..4842
                     /locus_tag="U4A13_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEN1780680.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="U4A13_002417"
     CDS             4913..6382
                     /locus_tag="U4A13_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1780681.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="U4A13_002418"
     CDS             6392..6859
                     /locus_tag="U4A13_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1780682.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="U4A13_002419"
     CDS             6895..7767
                     /locus_tag="U4A13_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1780683.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
BASE COUNT         2688 a         1184 c         1603 g         2384 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaa
//
LOCUS       DASJPC010000106         7697 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJPC010000106 DASJPC010000000
VERSION     DASJPC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.106"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..179)
                     /locus_tag="U4A13_002420"
     CDS             complement(<1..179)
                     /locus_tag="U4A13_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN1780684.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="U4A13_002421"
     CDS             complement(192..1295)
                     /locus_tag="U4A13_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1780685.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="U4A13_002422"
     CDS             complement(1385..2341)
                     /locus_tag="U4A13_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN1780686.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="U4A13_002423"
     CDS             2622..4556
                     /locus_tag="U4A13_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN1780687.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="U4A13_002424"
     CDS             5013..5429
                     /locus_tag="U4A13_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN1780688.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="U4A13_002425"
     CDS             5443..6009
                     /locus_tag="U4A13_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN1780689.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="U4A13_002426"
     CDS             6032..7018
                     /locus_tag="U4A13_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN1780690.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="U4A13_002427"
     CDS             complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="U4A13_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN1780691.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2203 a         1473 c         1591 g         2430 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgt
//
LOCUS       DASJPC010000107         7561 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJPC010000107 DASJPC010000000
VERSION     DASJPC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.107"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(166..495)
                     /locus_tag="U4A13_002428"
     CDS             complement(166..495)
                     /locus_tag="U4A13_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN1780692.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(485..757)
                     /locus_tag="U4A13_002429"
     CDS             complement(485..757)
                     /locus_tag="U4A13_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN1780693.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1208..2011)
                     /locus_tag="U4A13_002430"
     CDS             complement(1208..2011)
                     /locus_tag="U4A13_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN1780694.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1989..3113)
                     /locus_tag="U4A13_002431"
     CDS             complement(1989..3113)
                     /locus_tag="U4A13_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1780695.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(3129..3944)
                     /locus_tag="U4A13_002432"
     CDS             complement(3129..3944)
                     /locus_tag="U4A13_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1780696.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3934..4806)
                     /locus_tag="U4A13_002433"
     CDS             complement(3934..4806)
                     /locus_tag="U4A13_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1780697.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(4891..6180)
                     /locus_tag="U4A13_002434"
     CDS             complement(4891..6180)
                     /locus_tag="U4A13_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1780698.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6408..7052)
                     /locus_tag="U4A13_002435"
                     /pseudo
     CDS             complement(6408..7052)
                     /locus_tag="U4A13_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2322 a         1626 c         1163 g         2450 t
ORIGIN      
        1 ccagaatgtg gttcattctg acccggattt gattttgcac aactcatcag tccttattga
       61 caaagagcca aaaatctgag ccagaatgag gttcattctg acccagattt gattttgtac
      121 aacccatcag tccttattga caaagagccc ttctttttag tattattata attcttccat
      181 gcgttcttcc agtcgctgta aatcgcttgg acttaattcc ccaatttttt tccatttgac
      241 ctttttgttg tttttgggta attcgtttag agttcctacg tcaatccaag attctttttt
      301 tagtcctgcc tgtttccaat cccttatttc aacgtatttc agctttattt tatcggattt
      361 agtatgatat tttgaagtaa ttgacagaaa cgaaattgtg tccagtccat tttttagaat
      421 atagacaggc cggcgtttac cgccctctgc agctgtgaaa gcaacgtaga cggttgctat
      481 atcattagta ttcatcttca ttgaaccatt cttcaatttt ttcgtcagtg tctagttttt
      541 ggacaggcaa tttttccgct aatctttgaa tattatggat tcgtttactt ttttccgaca
      601 attcaataga aaatgggatt tttccttgct caacaatttg cttataaaaa acggtgacgg
      661 ctgttttggg gctcatgcct agttcatcaa taatgctttc tgcttcattt ttcaattttt
      721 cgtccataga aatttgtaac ttagccattt tctccacctc tttctgacaa acacattata
      781 tcacggaaag agttcaaatg gaattaatac aactactttt gaactccttg aaatgcagtg
      841 tatttatcta aatataagga aaggatatag tgctaaatgg ccttgataga tagaaaagtt
      901 tcaaaatccg ccacagcgat aattgaagcg aacactcaac gacacacaga acaggaaaag
      961 actcctaaaa gaaccaaatt aactaggacg tgtctgaaaa ctccacaaag atgtggtatg
     1021 ctctggtaaa aaacgaatgg cacaaccacg aagaccgata tggttcttgg aaaactttct
     1081 ccaccctttt tgtaattgga gggatagtgg tttattgata gcgattttcc aagttcttca
     1141 agtggaacca gacattgagc accacggcta aaaagtgaat gaagtcttta tttttaatca
     1201 ataattttta atgattacca gtaattggtt ttttcatgcg ccctatttct atttgatgtg
     1261 gagttaaata atgctgataa gtaaaaattt ttccgtcaat taaactgcca ctagtgaatc
     1321 ctgttctatt ggtaaaaaga gaatagtctg ggtattcatc tacaccaaat gataaagatt
     1381 cagccataaa ctgtttttta ccagcaaatt cagcactgct accttgatgg atatgtccta
     1441 caaaaatacc taaaatatca ctttcggcaa tcaattcagc aaaaccttcc ggaacttcag
     1501 ttacaataga tggatcttcc cacgacaatg gatgatgaac caataaaatc gttccctttt
     1561 cagcaggtgt acccaaccac tcttgtaatt gaatcatctg tcctatcgat atttttcctt
     1621 cgtgctgggt atcttgagca gaatccaagg aaataattcg taacccattt tgattcgtac
     1681 aatggatata ctctcccgtt tcattgcttt caacactcat tccttcaaag aaaacactgc
     1741 gacgatcatg attcccacga caaaaatagt aaggtgtggt tgggaaatat tgatccaaca
     1801 ttttccgtaa gacttgataa tcagaaatcc ccccttcatg tactaaatca cctgttaata
     1861 gaataaaatc gtacactgtt aacgagagtt cttgtaacaa tttttccaat gccaactgtg
     1921 gatttttaat tgttggaaat agttcagcag taaacaagtt agtttgatag ttttctctaa
     1981 aatggatatc agagagatgc aaaaagcgca tggctattcc ctctttcttc aataaaataa
     2041 tcaggatatc ctaaattttc ttgcgtctga taatcaaaaa aatgcattct ttcaggttct
     2101 atgcggagat tgactgcatc accaatctgc caagtaggtt tttcattaag gacaatcatt
     2161 tctataccat cgactttaac ggtgtacgca taactttgac ctaatagctc acgatacttc
     2221 acttgaccgt gtaaatcgct tgtttctttt gaaaaggtta cgtgttctgg tcttatgcct
     2281 aaatacaatg agccactaac ctgaagaagt tgtttttgcc aagctggatt caatgttaaa
     2341 aattgactgg ctacctttaa tgttccttga tcataagtaa ctggcataat attcatgcct
     2401 ggcgaaccaa taaaatttgc agtaaaagta tttttaggtt tcgtataaac atttttcggc
     2461 gtatcaatca tctgaattct accttcattc aataaaacaa tccggtcagc taacgtcatc
     2521 gcttcaattt gatcatgagt gacgtagaca aaagtttgtt tataacgttg atgaattttt
     2581 accaattctt ttcgtgcatc caatcgtaaa cgcacatcca aatttgataa cggttcatct
     2641 aataaaaaat aattactccg tttaaccacc gcacgagcca atgctacacg ctgccgttgc
     2701 cctcctgaca aatctttagg aagtcgatct tggtatggcg ttaaacctaa catcttcaaa
     2761 gcttcttcca aacggtgctg aatgtcagct gtcttaattt tattcgccct taatgcaaaa
     2821 ataatattct cctttacagt catgtgagga tataaagcat aattttgaaa aaccattgcg
     2881 atctctcgtt ctccacaagg tagcttattt gctagttgtg tacctaaaaa aagctctcca
     2941 ctcgtaattt tttccaaacc agcaatcatt ctcagcaaag tagattttcc tgaaccagat
     3001 ggtcccagta acacaattcg ttctccagcg gccacagcta aatcaatttc ttttaaaatg
     3061 gtatgttctc catatttttt tccgatattt cttaattcaa gcgtttctcc catgatttgt
     3121 cctccaattt atttaatacc tgcagaaaca aaagtcccga taatatattt ttgaaaaatt
     3181 gcatacaaga ctaacggtac aaacattgta attaaactaa tcgccattgc tacggtaaaa
     3241 tttgtcccac cttcagaact aatataggtc tgcatcgcca acggcaaagt ataattatct
     3301 actgtccgta aaattaatac cggccaaaca tactcattcc aagaggtaat aaaaaaccaa
     3361 ataccggtag aagtaatcgc atgtctagta agaggaaaaa caattttact catgacggtt
     3421 gtttgtttga tgttatccaa tgccgccaca tctagaaggc tcactggaat ttcccgcatt
     3481 gtttgcgtca ataaaaaaat gcccatcgca tcagcaaatt gtggtattat cacaccccaa
     3541 gtagtatcaa ttaaattaag ttttgcaatc aacaaataat taggaatcat cgcaacagta
     3601 aaaggaatga acatcgtacc aatgatgata aagtaccaga ttttcttccc tttgattcgg
     3661 caataagtaa aactatatgc cgcaaaaaaa gctacgccta acttaacaac cgttataatt
     3721 gtagcaataa aaaaagtatt tctcgtaatt ttgataattg gaagattttc gaacaaatag
     3781 caaaaatttt ccaaggtaaa cggctgtggt atcaaactag taatcgtctg ataggcatct
     3841 ttcaatggct taaaagaatt ggccataata aacaataatg gatagataat aatgattatg
     3901 atgaacataa ataagagatg tggcaaccag cgtttaattt tcatagtaaa cccccttttc
     3961 cacaaattta aatattaccc ctaagaaaac agcaaatatt aataaagtta aaactgaaat
     4021 tgctgaagat tctccgactt tatacatttc aaaagccttt tgataagcat aatagatcac
     4081 gtttgcacta ccattattcg gtccaccacg agtgatgatc ttaatcggag taaacacata
     4141 ctgcagacct tgaacaatgg tcatcaccaa taaatatagt cctgtcgaac tactcatagg
     4201 caacacaatt gtaaaaaaaa tgcgccagat gggtacacga tccaacttag ctgcttcgat
     4261 catctcttga tcaattccca caattgctgc aaaaatgaca atgaaattat aaccaaaaac
     4321 tttccaagca gtaatacctg caatcacagc aattacccaa ccttgtaaat tcatccacat
     4381 aggcagtctt aaaccaaaat ttcccaacaa gacagcaatt ggtcccgata ccggatttaa
     4441 tatccatgta aaaaccatcc ccgccactac caaagaaatc acagctggaa taaaaaataa
     4501 tggtttatac aaattctgcc ctttttctat aactattcct aataaaaagg atgtaatata
     4561 tgggaacaca cagctcagca agacaaatag aacggtatag gacaaagtat tcttcaacac
     4621 tttatgaaag actggatcag tcagcacctt tacatagttg gcaaagccaa caaattcttt
     4681 agtgggggcc accatattcc agtcaaaaaa actcaaatag atcgtataaa tcaatggata
     4741 aaaaacaaac acgcccaaca aaataattgc tggcgccaga tacaaataac ctacatagcg
     4801 cttcatttcc gacagcctcc tcatccttat gtcaaaagca gacgaccttg tttttccttt
     4861 cttacaggcg ctgtcgtctg cttgctttgc ttatttgagt aactcattaa tttccttttg
     4921 agtagctttc atcgtttcat tgacggaatt acctgataaa attttatccc gcatatccaa
     4981 caatttttgt tcagcttcta atccagcatc tccaggaaat gaactccaac tgaccatctt
     5041 atccatctga tcaattgctg gtatcatcat ctgattttca gctaagaaag cttttaatcc
     5101 attttgggcg tctgcaacat ctttacgtgg tggtaaataa ccagtacctt ctgtccattt
     5161 agctaaagat tcaacactat aaagatattt taagaaatcc caagttgctt cctttttctt
     5221 atccgtttgt gatgtaacag ctaacattgc gccaccagca ggtagtttaa cttttttccc
     5281 ttcccaactt ggtgactcaa ctgctttagc agtaaaagta ctattttttt gaatgttgtc
     5341 cctttgagca atagtggtat agaccatcgc gacgttacca tcaataaagc tttgcacacc
     5401 ttgttcccac gtagtgtgta atgccgtctg gtcttttacc atcctttgat ataattcata
     5461 agcagcgata ccatctttag aagcaaaggt agctttgcca tcttttatta cacttgcccc
     5521 attactttct aaaattgctt gttgtgccca attatcagca ggttcttgaa tatacacgcc
     5581 atactttcca gttttttctt ttattatctt agaaaaagtt tgaacttctt cccaagtttt
     5641 tggaccattt tcatccaagc ccgctgcttt taatatatct tgatttaaat ataaaaccgg
     5701 attactaatg gagtaaggaa tacccacttg tttaccatct ttttcagaca gtgccatgat
     5761 attgtctaaa aaatgatctt taaaataatc agcattatcc ggatccactt cttctactaa
     5821 agattgagga ttagtatatt tgaaattttc tgcaaaataa tctttaaaag cccatcccac
     5881 ttgtactacg tccggtattt ctttggaggc aacagaagct tgtaaatttt gcatcaagcc
     5941 tttatacata tcaggattat attttgctac aaccttcact ttgtcttgag attgattgta
     6001 tttatctact aattcattca ctgtttttcc acctagggta tccgcattaa catgccaata
     6061 ctctatcgtt acacgaccct ccgatttttt tgttgtcttg gtattcccac taccacaagc
     6121 tgccaaagag atgacactta aaacagacac aacccctata acaagatttt tctttaacat
     6181 aacattaact tcctcctaat ttctttatgg ccatttttct aatcaagaaa tattatgaac
     6241 taccaatgta aatttctagt taattttctg taaataattc gaaatttgtg catttcgggg
     6301 tacagggtca ctggacatat ccatcaagct aagaataaag cccccatatg gtcatgtatc
     6361 ccatctccaa atctcttaac tcctctgttt tgattataaa atagtttatt ctgtgctcct
     6421 ttcttccata aataggccca tgcacaacgt gaggttatat tataaacacc acagcgcgca
     6481 aggtactcaa tcgtttctag ttcattgact gtccatacag aaaatgtcaa tcctagctgc
     6541 tgccccactt cacgggccaa cttagttaac catccttcat gaatattgac gatttgaacg
     6601 ctttgatagg ctaatttctg taaggtatcg atcaaatctg cttcggtaat atccccaagt
     6661 tttttccctt cgaactgatt ttcaatatta taaaatatat gctttcccca aatcgcagcc
     6721 caagataatt gtacatagcc actaagaaca atttgtggcc agatttgata ggtttgtgcc
     6781 agcgaaaaaa cggccatttc caatccctct tcttttaaat cacagttaat ggtcatggtt
     6841 gccttctcat tggccactgc ttgaaacacc tgctcaaggg ttaccgaatc tgggacaact
     6901 tcttacctgt catgggctag tattagcgta ccatctgttt gctgacgcac atctacttca
     6961 aaacaaagct ggcgatccaa acaaaagcga agaaatgccc agctattctc ctccgtattg
     7021 tcactaccag agtgtgcagt gatcatggtc attttgaccc ctcctttaat tccgtgattt
     7081 tctcccacac gtccactata ccgatgaagc gttaatttct tgttaatttc ttgtaaattc
     7141 cctgcgtttt ttcagtaaac tagttgtaaa tcttgcatgc tccttcctac gaactcatga
     7201 atcgtcgatt ttgcccttct agctagtttc accaattaag tagttctaca ttccccgctt
     7261 ttaacctact acaccttttt ttgttttcag ctattccttc ttcttgctga aagtccttga
     7321 ggttctttct tacaggatac catcaaggtg ttcaatggct tattcccatt gaatgaccga
     7381 gacttactat cagatttctg ctcgttgaac caagtctttg atacaggatt tcttccgtca
     7441 tcttctcaca aataccacct tgcgtttctc tagcgattgg tcgatgtgca ccctcccagt
     7501 ggactgtcac tacccctttt tgctatgcct agcccacaaa aaaagctgtc ctcacagaca
     7561 g
//
LOCUS       DASJPC010000108         7352 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJPC010000108 DASJPC010000000
VERSION     DASJPC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.108"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            153..1631
                     /locus_tag="U4A13_002436"
     CDS             153..1631
                     /locus_tag="U4A13_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780699.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            1908..3968
                     /locus_tag="U4A13_002437"
     CDS             1908..3968
                     /locus_tag="U4A13_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN1780700.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4051..4620
                     /locus_tag="U4A13_002438"
     CDS             4051..4620
                     /locus_tag="U4A13_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780701.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4795..5601)
                     /locus_tag="U4A13_002439"
     CDS             complement(4795..5601)
                     /locus_tag="U4A13_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780702.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5739..7253
                     /locus_tag="U4A13_002440"
     CDS             5739..7253
                     /locus_tag="U4A13_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN1780703.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2642 a         1204 c         1440 g         2066 t
ORIGIN      
        1 attttttgca atattctcaa aaaaaagata aaaaatgaga ataactgatg aaaaaacaag
       61 cataatccat cataaaatca gtataggtga aaagatgaac tttcatctgc atatcaagta
      121 attggtatga ggcatttttt gaggtgtagg atatgaagtt ggattacctt atagaagagg
      181 aagatagaga aaaactgctg ctagctagat atttagaatt tgagaaaaag aattatgtag
      241 tgctgcctaa gttatatgaa acattaggtt tgtctaaatt taaaacaaga aattatatca
      301 tagaattaaa taaagaattt actgaattca aaacaaagcc agagatccaa atttcagaag
      361 aagcagagat ccatgtcgaa cgtctgaatt tgacggatat caaaaattta agaagaatct
      421 actttcaacg atcaaaaatt gctcgactgt taacggagat cgtggattca aaagtaacga
      481 tcgaaaaatt tgcagagaat cattttttaa gcatatcaag agcctatgta aaaagaagag
      541 aattagtctc ttttcttgaa cagcatcaaa taaagttaaa gaaaaacata ttagttgggg
      601 aagaaataaa catacggaac gtattatttg gggtatattt cggggtcttt aatggttttt
      661 ctatgccatt tagagaaagc gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat
      721 atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa attcaatttg ctaaccacta
      781 ttagtttgtt gagggcctat gggaatcaac caatcggaaa acgtttcttt tcagatgaat
      841 actttaagag ctcagaagga atgaagatca tcaaggattt atcacatatt tcttttcttc
      901 atatccctaa agagcagatc attgatgaga tttcttatat catgttgttt ttgatcacgg
      961 aagaagaatt tgaattagat ggcgcatttg gacttgcgga agctcatttt catgatatcc
     1021 acatgattag tcaaaaaatg tcagtggaat tgattgatca attaccattt tcgattgaaa
     1081 tctctgaaga agaaagacta tatatacgaa aagaacttcg tatgggtatg atgcatgtca
     1141 atcgaaaaaa ttggatattc gactttgaga tgactagctt tacgtctaaa aagcagctac
     1201 agtttttctt cgagacgtat ccaatattca gtacagcaat ccaaaaggtc atacaagaga
     1261 ataaacgcta tttcttggga aaagatgaac gtttcatgat tcgcttattt tatgattatc
     1321 tctttttatt ggtcggtatt tgtccggtat catattttga agcaccgatt catgtctgta
     1381 ttgatttttc gcaaggatct gcttataccg attacattat ttctcaagtg aaaggattta
     1441 aaaattacaa tattgtgatt gagagtcgaa tcacttctcg tacgaatatt tatttatctg
     1501 attgtttact tgaaaaattt aataaagaac agataatttg gaaaaatcca ccgactccag
     1561 atgactggga atattttgga aataccatca tcagagaaaa aaatagacat ttaactgaaa
     1621 aaatcagcta gtatttatgg atggcagctt tgtctcacat atttattagc tgatttatgt
     1681 ttttttcaaa gaaaaggtaa ctagatataa ctagatataa ctagatatat tacttaacaa
     1741 aattaaaaag tacataagtt attattccga ttttcttgaa acaataaaaa atcaaaaaaa
     1801 atactctcct aatagtccat cttcttttga ttttatttat ataatgaaat catcttatga
     1861 aaattactac ttcatgttta gacaagagta gggaggacta agaataaatg tgttcaaaaa
     1921 gacgttcaaa acaactaaat aaacacgctt gtcgtttaga taaggaacgg tcgtgttctg
     1981 aaaaacacta cgttaagttt atgatgcgca cgatgctgat cagtttatct gtaggtgtct
     2041 atatgcatgt acccgttcag gcaacgacac aaaccatcaa ggtcgtacgt acctctgttt
     2101 ctgagaccac tgattcccct atcactatta ctcatacttt cactgatgca aatggaaaac
     2161 agctacttcc tgcattgact tcctataata aagtcagtta tcctccaacg ataaaccact
     2221 actggttaat tcctaacaaa acattatgga catcggaaac cggaagcagt gtgccgcttt
     2281 ctatcttttc cgatatgact gatcctacaa aaggtgatca gttaaaacag atcactgata
     2341 ttttgaatca tgctttgaca gatataaagg acggtggaag cgcacaagtt tcttatgtat
     2401 atgaaaaaga ttattcacag tttgaaagaa agaaaatcga tgttctggca gggacaaaag
     2461 tttcgatatc tgatctggta gaaaggctga agaacgcaga tggaacacaa ggagatatgt
     2521 ccaaagtaat ttcaaacaat gggaatatcg tggatacgac taatacagga acatctacta
     2581 tttccttgtc ctattttgat ccaattgctt taaaaacaat gactgcatct gctgtgatcc
     2641 atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct tcacttaaga acaggagaga
     2701 gttacgcccc taaagatctt gttgaatatg taacaaaaag cgatggaact tcaggaaata
     2761 aagaagaaat caaaatgaac gggacagtag atactacaac tccgggagaa tatcccgttg
     2821 tattaactta tatagactcg aaaacatcaa atgtggcaga aacaacagct gtagtttatg
     2881 tagaagcatc tgctgcttca tcgactgtca tcactcattt tatagatagt caaacaaata
     2941 aagaacttca agcagaaaca catatgagta gtgtaaatcg tattcctgat ttgcgcctac
     3001 ctgctgaatt ttctgatcga gtgccaaata tagatttatc gacttatgaa caaaatggga
     3061 aagtcacttc tatttctgat tggatgcaag caaacggatt agacagaaaa agtcactggt
     3121 cggagatcct tgaagtactt cactcagaac tgatgaataa taggaagcaa tcaggagggg
     3181 aaattacctt tacttatgtt tatataccag acaatcgttc attagaaggt catgatctta
     3241 ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat ccaatcagca acaaattcct
     3301 taggtaaacc cgtaccgtta aaggatgtat cggttacaat caatgaagta ccattggagg
     3361 atggttattt gaccaaacaa acaggtgatt ttacgattat gtatagttat catgacccgt
     3421 attcactcca agaactccat gctcagtcac tattacatgt tttaccatta aacaaacaga
     3481 tagatcaaaa agaacaaaaa agacgagaag aaagcttgtc agaaaaaaaa caacaaatgg
     3541 ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag aagcctagag ttagcaagaa
     3601 tatctagaaa aatctcagaa tctaataaaa cgataaggca agaagctcat ttggccccac
     3661 aagtttctgg attggaaaat gaaattttag agcctaaaat aaaacaacaa tcagaaaaag
     3721 aatttgttgc taaacagtcg gaagatcaga aggaatcaga tgaccagaag gaaacgaaga
     3781 aaaaacgctt gtcaactatt gattccaccg atgagcatct gcagccgcaa gtaccacttc
     3841 ctagtccggg aacacccgca cctggtggag gtattccttc ttctgcttta ggagatttca
     3901 tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa tgaatttgac gcagatgact
     3961 ggttctgatt tatatctcta tctgttatag tcaaataagt agctgagtca tatgttattt
     4021 ggcataatgg agacgaggag ggatagaaaa atgttagata aaacgttacc ttacgcagaa
     4081 atctggatga caagaccact tcaacaaaat gttccaatca cgcctttagc ggaaggtttt
     4141 gaatttcgat tttatcaaaa agaagatgaa tctgcttggg cggagattga gacttccgtt
     4201 ggggaatttc aaacgacaga tgaagcactt gagtattttc aacaaaactt tgctccttat
     4261 cctgaagaac ttgaaagaag gatgtttttt gtagagacaa aaaaaggaga aaaagtagca
     4321 acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt atccggtttt tcattggtta
     4381 gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag cattgactgc cagagtcttg
     4441 caagaatttc ctaccctcca tacggacgga ccaatctatc ttcacacaca gacatggagc
     4501 catcaggcga ttgctcttta taaaaaaatg ggttttactt ttattgctga aaatctagac
     4561 ggcacaccta atccagatta tgaaaaagtt atgcaggttt taagtaaatt agataaataa
     4621 cgaaatggtg aaataagtcc tcagtattgg gataaaaaac gaaatagtca ttcctcatat
     4681 cttacttttt tgagttaata ctatcggata actaatggca tgccatacat tcaaaaaaga
     4741 aggggcagtg acaaaagtat tgtcactgcc ccttctcttg ttttttagca tgttttatcg
     4801 tttgattttt ttaggaataa aattcttgat gaacgtaaat ttttggtaat cgtttcgact
     4861 atagaggtaa ccaaatacac tgaaaacaac tgctccaata aaattaacaa ataaatcctt
     4921 catagtatcc atgattccca cgtctaagta accattttgg atgattgttt tatctgtttt
     4981 accatctgca ttttgacttt caatgattgt ccgagtgata tctggaacat gatagacagt
     5041 gtttccgtct ttacttaact ccacactatt gatttgctgg atgatcgtat ctttttgcat
     5101 atcgaattgc agtaatttat ctgctgaaaa ttccacaaac tcccacataa caccaactgt
     5161 catcgaaaaa cagaaggaaa caactgctag taataaagga gtcaagttca tggtttttgt
     5221 atttttattg agcaaataaa caagggaaaa gccgacaccc ccagctaaaa atccgctgaa
     5281 tgcatgtaga aaagtatccc acattgaaaa atagccataa aaatcactta actctcctaa
     5341 aactgttgaa ctaaaaataa aagataacaa aatgatttct agcatatttg gaatctgtat
     5401 ctttaatatc ttttccagaa aataagggat ttcaaataaa attaatgtca gtaataaaag
     5461 cattgtattt ccccagtgtt ggttccagac ctgatccact agcataataa cgacgatgat
     5521 acgtaataaa atatagattc cgtttgtgat tctacctgca gtttttgtac tgttcgtaaa
     5581 aaaggttttc aaatgattca taaaataact ccttcatgta tatccttaat tatactaaga
     5641 agcgtgtgtt taccaaagct atatgactta aagaaaaaca gagttgatat tgtgaaaaaa
     5701 atcacttaat atagtataag gagaaaaggg ggatacaaat gaaaaaatta gttgctggtt
     5761 tattaacagc cgttgcactt cttgctatga gcgcatgcgg cgcagatcag aaaaatacag
     5821 cgcagacaag ttctagtacg aaggaatctg ccgaagtatc atcaggcgct tctgaacaag
     5881 tatatacaga tccaaaagaa atgaaagaat cttatgatgt cgtgattatt ggagctggtg
     5941 gtgcagggat ggcagcagct atcgaagcaa aagatgctgg gttgaatcca gtaattttgg
     6001 aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc aagcggtatg aatgcttcag
     6061 aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa tgatgccttc tatgaagaaa
     6121 cgctaaaagg tggtggagga acgaatgata aagaaatgct tcgatacttt gtcgatcact
     6181 ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa actggacaac ttaacgatca
     6241 ctggagggat gagtgaaaag aggacacatc gtccgtctga tggctcggct attggtggat
     6301 acttagtgga ggggttattg aataacgtcc atgagcggga aatccctatt tttgtcaacg
     6361 cagatgtgac agacattaag aaaaatgggg aaacggtcaa tgaagtgact gtacatgttc
     6421 aaggagaaga accaaaagag gtcaccggaa aagcaatcgt agttaccaca ggcggttttg
     6481 gtgccagcaa agaatttatt gaaaaatatc gtcccgattt agctgattat attacgacaa
     6541 atcaagaagg atcaactgga gatggtatca ccatgattga aaaattaggt ggacaaaccg
     6601 ttgatatgga taaaatacaa attcatccaa ctgtgcagca ggatgagggg gtcttgattg
     6661 gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca agaggggaaa agatttgtca
     6721 atgaaatgga tacaagggat aaagtatctg cagcaattac agcattgcct gaaaaatcag
     6781 cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac agctattgaa ttttatgacc
     6841 aaaaaggata tgtcacaact ggaaagacaa tggaagaatt agcagaaaaa atcgatttac
     6901 caaaagaaac actagcaaaa acagttgatg gatggaatac ggatgttgcg gagaaaaaag
     6961 atgctcaatt caaccgtgcc acagccatgg aacatgactt atcgtctccg aattattatg
     7021 cgatcaaaat cgcaccagga atccattaca cgatgggcgg tgtgaaaatc aatacgaaca
     7081 ctgaagtatt agataaagaa aaccagccaa ttagggggtt atatgctgct ggtgaagtca
     7141 caggtggctt acatggaaac aaccggatcg gtggaaattc agttgcggag atcattattt
     7201 ttgggcgaca agcgggcata caagcagctc gatttgcttc ggaaaatgaa tgaaatagaa
     7261 cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta ataccgaggg tcagctgatt
     7321 gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DASJPC010000109         7327 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJPC010000109 DASJPC010000000
VERSION     DASJPC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.109"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..661
                     /locus_tag="U4A13_002441"
     CDS             <1..661
                     /locus_tag="U4A13_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN1780704.1"
                     /translation="LNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFV
                     YAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMG
                     IDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNS
                     GALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSAL
                     F"
     gene            648..1592
                     /locus_tag="U4A13_002442"
     CDS             648..1592
                     /locus_tag="U4A13_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN1780705.1"
                     /translation="MHYSKEEKNEKKKVERVQVRSFNKTANFFSNIMIALFAISCILP
                     FIFVIAISFTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGT
                     LINSTATSLYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWA
                     LILPMAVSPFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATIS
                     LFAALGYWNDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVP
                     GESARMAIVVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1633..3090
                     /locus_tag="U4A13_002443"
     CDS             1633..3090
                     /locus_tag="U4A13_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN1780706.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3435..4049
                     /locus_tag="U4A13_002444"
     CDS             3435..4049
                     /locus_tag="U4A13_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN1780707.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4042..5766
                     /locus_tag="U4A13_002445"
     CDS             4042..5766
                     /locus_tag="U4A13_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1780708.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5763..7214
                     /locus_tag="U4A13_002446"
     CDS             5763..7214
                     /locus_tag="U4A13_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1780709.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         2500 a         1219 c         1467 g         2141 t
ORIGIN      
        1 tttgaatctg ctgatctcag tcgtatttgc aatcattatg agcgagttga gaaataaacg
       61 actcgttaaa gtttatcaaa cgatgtcctt actaccttat tttctatctt gggtcattat
      121 cagttacttt gtttacgctt ttttaagtcc tgacaaagga atattcaatc agtggatcac
      181 tggacatgga ggagaagctg tcaattggta taatgaaccg aaatattggc cgtttatctt
      241 ggtgttcatt ggtacatgga aagggattgg ttataacagc attatttact ttgcatctgt
      301 tatgggaatc gatccgactt attatgaagc tgctatggtt gatggagcta gcaaatggca
      361 gcagatcaaa catgtgacga taccacaact tattccacta atgacgattt taacgatttt
      421 agccgtagga aacattttcc gagctgattt tggcctgttt tataacattc ctaggaattc
      481 cggtgcatta tatgatgtga ctcaagtttt ggatacgtat atctacaatg gcctgacatc
      541 cacaggagat tttggtatga cggctgctgc aggactttac caatcagttg tcggctttgt
      601 cttgttgatg atcacaaata cgatcgcccg acgttttgat agcgattctg cattattcta
      661 aggaggaaaa aaatgaaaaa aagaaagtgg agagagtcca agtccgctct ttcaataaga
      721 cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt attttgccat
      781 ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact cacgggtaca
      841 gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac cagatgcagg
      901 ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt acactgatca
      961 atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt ccattccgtc
     1021 gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg gtggccaatt
     1081 atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg atcttgccaa
     1141 tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt tcggtctctg
     1201 attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata tttgttcaga
     1261 tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc gcattgggtt
     1321 actggaatga ctggttcaat gctttactat acatccaaga tgataagcta gttccactac
     1381 agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat tcaggagcag
     1441 gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga atggcgatcg
     1501 tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag cattttgtca
     1561 aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat cataaaatta
     1621 ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc agcgttgttg
     1681 ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga agctagcaat
     1741 tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga tcaattgatg
     1801 gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat caattacatt
     1861 ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga aaattatgat
     1921 atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc tgatttgact
     1981 gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc atatattaaa
     2041 ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt ctttgctcaa
     2101 caagtactta cttttaacaa agctttacta gataaatacg atctttcaat tgatggaatc
     2161 caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga accaaacacg
     2221 gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta tcctttaggg
     2281 aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat caaccaatac
     2341 gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa acaaggactc
     2401 attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa tacttggcta
     2461 atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac aaatgcagca
     2521 aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc tcaagcacaa
     2581 atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt agaacttcta
     2641 gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat cgagggagaa
     2701 gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg ttatcagcca
     2761 aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac acaagagtct
     2821 attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga aacttcaccg
     2881 attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa tatttctaat
     2941 gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga tgaaacatta
     3001 ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa agaaatgcaa
     3061 aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg gttgtgacaa
     3121 aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt ttctcggtat
     3181 taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc ttactactcg
     3241 aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct aaccctcggt
     3301 attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc tctaataatc
     3361 aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata aacttaatgt
     3421 ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg atctgggtca
     3481 ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc atctttggat
     3541 taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc gagtataaag
     3601 aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga ggcaatctgc
     3661 tatttagttt atttttatct ggtactgctt tattgagtta caatttatat ttatctgttc
     3721 aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc ttgctctgcc
     3781 tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc tctgttccta
     3841 atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt ctaaaaataa
     3901 ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg atcctgtttg
     3961 gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg cgtgaagaat
     4021 tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa aggaacctta
     4081 ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat gactgctacc
     4141 gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa catgacaagt
     4201 gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag taggatcatc
     4261 aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta cttaacaaaa
     4321 cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga tgaattagtt
     4381 tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag tgcgatatat
     4441 atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa aggtggggaa
     4501 ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc tatcacacaa
     4561 ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact agatatgatt
     4621 ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg aacgacaaat
     4681 cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag acaagaaatg
     4741 atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct ggaaaaaaac
     4801 aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt actagataaa
     4861 aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg tatcattatt
     4921 ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc acagcaagta
     4981 tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac gcggatcgac
     5041 ataacaaaca cggaatatga actgagggaa ctttcaatcg gtatcaacga aatgttggat
     5101 agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca agacgcacat
     5161 atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt agaatatatc
     5221 cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta tgcgttttcc
     5281 gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga agaactggac
     5341 ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt agcctatcat
     5401 tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat ccagccatta
     5461 gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc gctaagtgtc
     5521 aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg aaaagggatg
     5581 actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt ggaacttcac
     5641 ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg cagcagttat
     5701 tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac attcaaggag
     5761 ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg aatgggttaa
     5821 aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca gagaatgcca
     5881 aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg gatgtgacaa
     5941 tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt cataattttg
     6001 aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt atgcaattag
     6061 gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa agcctcaaga
     6121 aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa atctatcaag
     6181 aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa gaagaaataa
     6241 taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca agaactcatg
     6301 atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat caacgaaatt
     6361 acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat ggcttctgtt
     6421 gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg gaagagacag
     6481 attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta cagttgcatc
     6541 aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa acaaaagatc
     6601 aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg gaagtcgctc
     6661 aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg ccggaagata
     6721 tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt gatctggaag
     6781 atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg caagaacttc
     6841 agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac agtgacaatg
     6901 tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca ctcaaagaag
     6961 tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa aaagaaacga
     7021 aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa caactgcttt
     7081 tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac aacaccacgt
     7141 atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac cgagaaaaca
     7201 gtggagaatc ataaaagaaa aagccaatgt aatgaaataa cttataaaaa attgtctaga
     7261 aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat gacctctcga
     7321 taaacgg
//
LOCUS       DASJPC010000110         7290 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJPC010000110 DASJPC010000000
VERSION     DASJPC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.110"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            77..2686
                     /gene="clpB"
                     /locus_tag="U4A13_002447"
     CDS             77..2686
                     /gene="clpB"
                     /locus_tag="U4A13_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN1780710.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2837..3523)
                     /locus_tag="U4A13_002448"
     CDS             complement(2837..3523)
                     /locus_tag="U4A13_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780711.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3501..3995)
                     /locus_tag="U4A13_002449"
     CDS             complement(3501..3995)
                     /locus_tag="U4A13_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN1780712.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4015..5235)
                     /locus_tag="U4A13_002450"
     CDS             complement(4015..5235)
                     /locus_tag="U4A13_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1780713.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5382..7217
                     /gene="lepA"
                     /locus_tag="U4A13_002451"
     CDS             5382..7217
                     /gene="lepA"
                     /locus_tag="U4A13_002451"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN1780714.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2398 a         1396 c         1498 g         1998 t
ORIGIN      
        1 accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt acctttaatt
       61 aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag aagcgattgc
      121 tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg cccatgtatg
      181 gaaaatcttt ttacaatcta atcattttgg tcgaaatttc tatacggatg ccggattgga
      241 tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag ttgtttctgg
      301 tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat tgaatgaagc
      361 agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg ttattttagc
      421 gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg gattatctga
      481 aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga cctcacaaaa
      541 ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac agcaagtcaa
      601 aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg tcatccgtat
      661 tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg tagggaaaac
      721 ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg aaaatttaaa
      781 agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa aattccgtgg
      841 tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg atggacgtat
      901 cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag aaggaagtat
      961 ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt tgatcggtgc
     1021 cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg aacgtcgctt
     1081 tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc tgcgtggatt
     1141 aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat tagtagcagc
     1201 agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag caatcgattt
     1261 gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa cggaattaga
     1321 tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga aaaaagaatc
     1381 agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg aacttcgtga
     1441 agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca attctgtttc
     1501 agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag aaaataacta
     1561 tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag aaaaagaatt
     1621 gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag agtccgttac
     1681 cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa cgaaacttgt
     1741 agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac gagtgattgg
     1801 tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg ctggattgca
     1861 agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg tcggaaaaac
     1921 cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata tggtacggat
     1981 cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag ctcctccagg
     2041 ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa atccttatac
     2101 aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata tcttgttaca
     2161 agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt tcaaaaatac
     2221 ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg tgactgctga
     2281 tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg gaaacttcaa
     2341 accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga gcctagacaa
     2401 cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg aacatcaaga
     2461 aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg cttatgaacc
     2521 agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa caccactagc
     2581 taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta ctttacttga
     2641 tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt ccattgagac
     2701 tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt caaataaaca
     2761 gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct gcccgcttct
     2821 gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt tcaaagtaac
     2881 gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca cctatcgtat
     2941 ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac agtgtgccat
     3001 cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag aattcccaga
     3061 actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg aacaaccaag
     3121 gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca tagccaagag
     3181 ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag atgatactga
     3241 tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag taaaacaaaa
     3301 cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc atgaaaatca
     3361 atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc gaatattttt
     3421 gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca agtaaagcta
     3481 tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa tctttttact
     3541 tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat caaacctatt
     3601 gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc agcgattttt
     3661 ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac tgggatgcct
     3721 ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag cccatctagt
     3781 aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc aatcattcga
     3841 ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag atagatcgaa
     3901 agctcagaag agagctctct gatttcttta actaactgtt ggttatataa caaatcactt
     3961 ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt tttatcaaaa
     4021 ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact tcagtgtttc
     4081 ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat aattcatcat
     4141 caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt catttaagac
     4201 acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc aaggagcgac
     4261 aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa accgttgaag
     4321 gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct ctttgatctg
     4381 actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat gtacccagta
     4441 gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt cttgatcaag
     4501 atagaacatt tcttttacgt gaaatatttc ttcagctacc cagtagaaca gaacacctct
     4561 tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat tttcgaaacg
     4621 ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt gttgggcaaa
     4681 tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa ccataccact
     4741 tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt tatcagacat
     4801 gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat aagagatcca
     4861 ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca tcaatccatg
     4921 gttagcatat tctggataag ttccaactcc agtcacacca cccattttat aacgtgcacc
     4981 gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat tttcgtggaa
     5041 ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa aagctcgttt
     5101 attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat aggaaagcaa
     5161 ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat tttctctaaa
     5221 atctttttga tccatttttt catctccatt tattagcttg ataacagtat agcaaaattc
     5281 tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg cttttcgttg
     5341 gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata aagaaaatga
     5401 aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac catgggaaat
     5461 ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc gaaatgcaag
     5521 atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt aaattaaatg
     5581 ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac ttgattgaca
     5641 ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc tgtgagggtg
     5701 ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc aatgtttatt
     5761 tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac ctacccgcag
     5821 cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat gctagtgaag
     5881 ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag cagatcgtgg
     5941 aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg atctttgact
     6001 ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac ggtatggtca
     6061 aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt acagaggtcg
     6121 gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat gtcggttata
     6181 tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta acgttagcag
     6241 acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg gtttattgtg
     6301 gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta gaaaaactgc
     6361 aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta ggttttgggt
     6421 tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc ttggaacggg
     6481 aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt aataaaacgg
     6541 atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta acgattcagg
     6601 atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt gtcggtgcag
     6661 taatggaact ttctcaacga aaacggggag aattcattac gatggattat ttagatgatt
     6721 accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac tttttcgaca
     6781 aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct ggttatcaaa
     6841 aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat gcattaagct
     6901 ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa aaactgaaaa
     6961 aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga caaaaaatcg
     7021 ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt tatggtggag
     7081 atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag cggatgaaac
     7141 aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa atggacgagg
     7201 acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa accatgttta
     7261 catggatttg ccaccggttt gccaccggtg
//
LOCUS       DASJPC010000111         7224 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJPC010000111 DASJPC010000000
VERSION     DASJPC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.111"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(70..690)
                     /locus_tag="U4A13_002452"
     CDS             complement(70..690)
                     /locus_tag="U4A13_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN1780715.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(766..1203)
                     /locus_tag="U4A13_002453"
     CDS             complement(766..1203)
                     /locus_tag="U4A13_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN1780716.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1366..2196
                     /locus_tag="U4A13_002454"
     CDS             1366..2196
                     /locus_tag="U4A13_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN1780717.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2180..3547
                     /locus_tag="U4A13_002455"
     CDS             2180..3547
                     /locus_tag="U4A13_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1780718.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3566..4375
                     /gene="yidA"
                     /locus_tag="U4A13_002456"
     CDS             3566..4375
                     /gene="yidA"
                     /locus_tag="U4A13_002456"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN1780719.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4418..4861)
                     /locus_tag="U4A13_002457"
     CDS             complement(4418..4861)
                     /locus_tag="U4A13_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN1780720.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4995..5366
                     /locus_tag="U4A13_002458"
     CDS             4995..5366
                     /locus_tag="U4A13_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1780721.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5535..6317)
                     /locus_tag="U4A13_002459"
     CDS             complement(5535..6317)
                     /locus_tag="U4A13_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN1780722.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6314..>7224)
                     /locus_tag="U4A13_002460"
     CDS             complement(6314..>7224)
                     /locus_tag="U4A13_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN1780723.1"
                     /translation="MKKCFFDYESGKISQNPRLVEILPDGKGKNMFAMMPAYLGENRY
                     FGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKALINANAVTWIRTAAVSALATDY
                     LANPHAETLALIGAGQQALSHLTAISTIRPITTVFVYDILEERRNTFIAKAKKQYPKI
                     TFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVREDAHINAVGTFTPDTREISTSLM
                     ETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGTLGELVSEKKTFYKGESRPTIF
                     DAVGLAIEDLCCAEYIYNKLEKGALK"
BASE COUNT         2185 a         1506 c         1259 g         2274 t
ORIGIN      
        1 atcgtgtcac acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga
       61 aaataatgtt tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc
      121 atcgtcttcg tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc
      181 atctgtcggg tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc
      241 atcttcatca tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc
      301 ttctgggtca tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac
      361 tttcttgcgt ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac
      421 ttcttcatcg atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa
      481 tgagataaag ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat
      541 ttcactgtct gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat
      601 gacatcgcca cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt
      661 gtttgcacct tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct
      721 tatcatacaa aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc
      781 caaggtcaaa taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg
      841 aaccttccct cctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc
      901 aatctggatc ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa
      961 tttcaaacgc atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa
     1021 tttgatcgtt acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta
     1081 cagcgtatcg ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc
     1141 cccttgctgt tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc
     1201 cattattcat tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc
     1261 aaccaacttg tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag
     1321 tataatattg tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt
     1381 cgactagatc agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata
     1441 ttaacggaat attctaaaga acaccagcaa atctttttac atttctggca atatgactgc
     1501 acagtcatct taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca
     1561 ctgaaagatg caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca
     1621 gatgaaggaa ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc
     1681 atagatgacg cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg
     1741 atacaagcat tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat
     1801 ggcaaaaaat ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg
     1861 tacttaagtg tcaacggtaa tcaggaaaaa cgcgggcaag tcatgaagcg attttatcaa
     1921 tctgctttaa aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg
     1981 gcaacacttg aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt
     2041 gcaaaagctt ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta
     2101 aaaaaagaaa tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat
     2161 aacataaagg agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa
     2221 agtttttcgt gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt
     2281 gatcaattcg aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt
     2341 cacttttcac ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc
     2401 aagacgaatc tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg
     2461 ctgggatgat caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca
     2521 tggtccttac tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt
     2581 agagatcatc acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg
     2641 ttttcctgaa aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca
     2701 aatgatttct agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt
     2761 cactggaaca gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta
     2821 tgaaggcggg atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag
     2881 ccgatatcaa atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat
     2941 tttggagcat ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga
     3001 actccattct caattacttc tgccatttct tgacggaagc tttactttag aagaatacct
     3061 caaacttgat gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc
     3121 tattttaaat gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt
     3181 ttctggcaac cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg
     3241 atataatcct gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta
     3301 tcgcccagaa caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt
     3361 gatagaatta tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga
     3421 tgagcgattt ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt
     3481 atttgacgaa acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat
     3541 aaattaaaat atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg
     3601 tactttatta aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc
     3661 tgttgcaaaa ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga
     3721 acagttaaac gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc
     3781 actcgtacaa caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga
     3841 tttcctagag atcgaagtga tggcacgccg tgtcggcagt catctccatt cgatcgatga
     3901 caaagcgatc tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt
     3961 agtcaagatg ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt
     4021 caaaatgatg atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca
     4081 aactttccgt gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa
     4141 taaagaagca agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca
     4201 agatgaaatc atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg
     4261 tctaggagta gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac
     4321 ttcatcaaac gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat
     4381 aaaaaaactg agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc
     4441 atctctcgac tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc
     4501 tcacccacca aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag
     4561 caacaaagaa atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact
     4621 catcagcttc tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct
     4681 cacggacaat cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa
     4741 aagcaccatt tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg
     4801 ctgcataacg ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca
     4861 tgaatagttt ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat
     4921 gaacaagcac aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa
     4981 ggagagatga atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat
     5041 caatcgaatt ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag
     5101 aaaacaaatt tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga
     5161 cttacaacta tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag
     5221 cagctgatga tctagaaaaa ttacacgagc aacataaagc tgccggtctt gagattactg
     5281 acttaaaagg attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt
     5341 ataaaatcga agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa
     5401 aaacaaacac caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg
     5461 tcttttttta acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat
     5521 tcatttgaaa gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct
     5581 attatcacac gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga
     5641 taagctgaca attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt
     5701 gctgatccac atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca
     5761 aagtcttttt gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg
     5821 atagctgtaa aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat
     5881 aaatcacgtg ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac
     5941 aatatcggca cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt
     6001 cttgtcacaa atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg
     6061 taatagttaa ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct
     6121 cgatctggcg taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt
     6181 tcagataaat tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct
     6241 agcacataaa ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg
     6301 taataatcta agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac
     6361 aacaaagatc ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt
     6421 tataaaatgt ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct
     6481 ctaaatctcc ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt
     6541 aatcatctac atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag
     6601 gagtaaatgt gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa
     6661 atgcctcttt actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta
     6721 ctgatattgc atttataaac gttattttag gatactgttt tttagcttta gcgataaaag
     6781 tatttcgtct ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag
     6841 aaatcgcagt caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg
     6901 catgcggatt tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg
     6961 tgactgcatt tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa
     7021 tctctcctaa atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt
     7081 ttgtgccaaa atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc
     7141 cttttccgtc aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat
     7201 aatcaaaaaa acattttttc atta
//
LOCUS       DASJPC010000112         6952 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJPC010000112 DASJPC010000000
VERSION     DASJPC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.112"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(295..708)
                     /locus_tag="U4A13_002461"
     CDS             complement(295..708)
                     /locus_tag="U4A13_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN1780724.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1164..1649)
                     /locus_tag="U4A13_002462"
     CDS             complement(1164..1649)
                     /locus_tag="U4A13_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780725.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(1788..2003)
                     /locus_tag="U4A13_002463"
     CDS             complement(1788..2003)
                     /locus_tag="U4A13_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN1780726.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2227..3027
                     /locus_tag="U4A13_002464"
     CDS             2227..3027
                     /locus_tag="U4A13_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN1780727.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3046..4914
                     /locus_tag="U4A13_002465"
     CDS             3046..4914
                     /locus_tag="U4A13_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN1780728.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            4907..5398
                     /locus_tag="U4A13_002466"
     CDS             4907..5398
                     /locus_tag="U4A13_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN1780729.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5386..5940
                     /gene="srlA"
                     /locus_tag="U4A13_002467"
     CDS             5386..5940
                     /gene="srlA"
                     /locus_tag="U4A13_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN1780730.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            5958..>6952
                     /locus_tag="U4A13_002468"
     CDS             5958..>6952
                     /locus_tag="U4A13_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN1780731.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2434 a         1110 c         1348 g         2060 t
ORIGIN      
        1 aatcctctaa tcttatggat aaaatagatc atcggacaag cagataagag taagtatttg
       61 ttaggccttt tgaataagta aaagaaagca ctggtcatta tgataaatgg tatccaaaac
      121 aaaagtttac ttaagacaat caaatgatca actcccatat tttttaccaa gagagatcat
      181 aacatcttta tcgtaaaaac aaaatatttt tttgctcatt ttgaaaattt aaacatactc
      241 gttcttatcc aaatatataa aaatcgcccc tcattcatcg agaggcgata agttctaatt
      301 taactcaatt ttttttgttt cttttggctg ataagctgtt ttaggtaata ccaatttcag
      361 cacaccgttt ttaaaggttc cagtgatttt ttcttcatca acattaggaa gatagaatga
      421 acgtttgtaa gagaccgaat gtctttcttt ccgtacatag ttgccatctt ctttatcttc
      481 tgtatgggac tcatgattgg cggagatcgt caaagtatta tctgaatatt caactgtcgt
      541 gtcttctttg tcacagccag gcatatcagc agttagtttg tattcttttt cattttcgac
      601 taaatctact tttggataac ttgaaacacc taaaaaatca ttgaatgctg gactaaacac
      661 gtcattgaaa tcagggaaca tgtcgcgaat gctaggtaca tttgccattt ttgatcatcc
      721 tttcaataag tattttttat ttatccctat ttacgtaata actctatttt gaaaaaaatg
      781 caagtataat ggttagcttt gtgaatattt catctaatac gatacaaagg ctatctttct
      841 aatttgaaaa agggatcatt aaaataataa gaagaaaata tgtcaaaagg taaaaaagta
      901 aattcttcta tccaaatata gaaaaattga atctagaaaa ccgcccgaca ataaatttca
      961 ttaactcagt tgaccgaaaa tgaagtagat accaaaaatt tatcaagaga gcaagtagag
     1021 aagtgagtat attcataaga gaaatagctg taacgaatta ttaggttaat gcaaatgctt
     1081 atttatactc cgtataactt tctcagaaaa aatgatacca ataatgactt gctagtactg
     1141 taaatccgta taatatcatc taatcaaaat ggtgttccat ttgcatctgt catcgtaatc
     1201 gatccttcgg gtgacactcg ataaaaacca acgcttccag atcctccttg ggaacggatt
     1261 tgtttgctgg aagctgtgaa caaataatct cgatcaattt tttgataaaa gctaataaca
     1321 atatcgtcac tttgtaattc ggaacggtgg gcaataagaa atagaagtgc tttgtcagag
     1381 ttttctaatg gaagagaagg tacagtgctt tcttgcattt cagatgttga agaaatgcta
     1441 aacgactgaa tacttgattg gctatgttgc gaatctacat taccagtaga agtattagta
     1501 atggaagtag tagaagtact tttagttgaa ccaacctcag ttgttgaaat ggctgctgaa
     1561 gattctttaa cagaattctt tgttttatat ccacaggcag aaaaaagtat tacagttccc
     1621 aacaagcctg cgtaaatccc tttatgcatg atagctcctc ctaaaataga ttatctttat
     1681 tcagtatatc acttaaacaa agaaaagcag atggtttaaa atgaaaaaac cttcccaata
     1741 aagggaaggt tcatgaagat aataactttt ttaataatta tagctttcta atatctttca
     1801 tcaacttttc tagtaatcat tgggaaaaat ttcaagcagt agatcgcaca aatacctacg
     1861 acgatataaa tgatcttcgc aaaaatagtt gttgaaccac cagcaatcat agctactaaa
     1921 tcaagttcga acaagcctac caacagccag ttcaaccctc ccacaatcaa taaagttaat
     1981 gcaatagcat ctaaagtttt cattagaatt ctcctttctt aaatgataaa acaattatat
     2041 atcaaaaaga gaaacattgc ttatataacg ctcaggaatt ttagcaatct atgcaaaaag
     2101 atgaaaattt tttttgctct tccattagta cacatttttt agctgaagct gtaaatttca
     2161 tgtcttaatt ttttttagtg agcttttata ctggatttgt aagaaaaaag aaggagagga
     2221 tcacatatgt cggattggtt ggatattgca ggaaaagtag taattgtaac tggaggttcc
     2281 tcaggaattg gaagaagtat cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat
     2341 tttgatgtaa ctgaatgccg tatacagcat gaaaacctac tatctttaaa agtcgatgtg
     2401 tcttctaaaa cggacataaa agaagggata tataaagtaa tgaaacattt taagacgatc
     2461 gatggtctag tcaataatgc aggaataaat attccttctc ttctaataga tagaaatcat
     2521 ccaaaaagca agtatgaatt atcagaacaa gtttttgata agatgatcgc tgtcaatcaa
     2581 aaaagtgtat atttgatgag ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga
     2641 gtgatcgtga atctttcttc tgaatcagga ttggaaggat cagaaggaca gagctgttat
     2701 gctgctacaa aagcagcgat gaacagtttt actcgttctt gggcaaaaga gttaggaaag
     2761 aggaatgttc gtgtggtagg tgttgcacca gggatactgg aagaaaccgg tttgagaaca
     2821 caagaatatg aagaagctct ttcttatacg agaggtataa gcgtcgaaca gctgagaaac
     2881 ggttacagtc gaacaagtac gatccctcta ggaaggagtg gtaagctgca agaagtggcg
     2941 gatctagtct gctattatct ttcagaaaga tcaagttata tcactggagt cacgactaat
     3001 atttcaggag gtaaaacaag aggataaagg atagggggga cgcacgtgac gctagtaaac
     3061 agatggtacc aattattgaa attactagtc actcacaaga aagtttcaat ccaggaagtg
     3121 caaaaagaaa tcaaaaccag taatcaaacc ataaaaaaag atattgggga gttgaatgaa
     3181 gaaattgccg gtattgcaaa aatcatccaa aaaaacaaac attttgaact tgaaatttat
     3241 gattttgatt tttttgatga gatcatgcaa ggaaaattga aaacagccag tgatttcaat
     3301 tcggcatcca aaagaatcgc ttatttacta aataggctga tcgaaacaga ggatttctta
     3361 cgtatggatg atttgtctga agaagtcggt gtcagcagag gaacagtggt caaggatcta
     3421 aagacattaa aagagatgat caatgatttt gatgtaaaga ttacaggcac accaaataaa
     3481 ggactaagaa taataggaga tgagctagaa ttacgactac ttttatttta ttttgtttct
     3541 ccctactttt cagagacgaa aacagaacag tttctagaag aaaaacaagc aatcaaacat
     3601 tttcagaaga agacgaatgc tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt
     3661 atttctttga atagaatctc tagtaactat ctgttgaaga agcccatcaa tcgttacagt
     3721 gggtgttttg aatatatcca agaattcaat gaactgatca atcagttgga agaaatctat
     3781 gaactaactt tgagccaata tgaaaaagat tttatcagtt ttcctttgaa catcatcaac
     3841 acaagtataa cgatgataag gaaagctaac tctgatttgc gcttatattt cgaccccatg
     3901 atgatgcgta ttcgacgatt agatctcatt gatctggatg agtccttctt atatcaagag
     3961 atgaaacagc acctctcttt attgataaat cgggtaattt ttcgttatcg attgactgat
     4021 ttattttatg gtgagataga aaaaaagtat cctttttctt accaaatcgc tagcgaatgc
     4081 atacaagcat tagaagctat cttgcaacat aagatctcta aagtagagat cagctaccta
     4141 acgttgtatt tggagttagc atttcgatat caaaaagagc atttttcctc taaaaaagta
     4201 gccattgttc atacgacagg aaaaggaaca gcattaatga tccaaagaca aatcaaaagg
     4261 atattgggga gggatataca aataacacat gtttcagaag aacaatatga aaaagtagat
     4321 ctaaacaaat atttcgctgt ttttacaacg attccgttaa aaaatgtaaa tgaagagaca
     4381 ccggtgatcc atttgactaa tctttttgat gactcttggt taagggcaca atggcaaaaa
     4441 gcaaaaaaaa agcgattagc cgggtttcat tctactgttt ttttctatca aatatggaaa
     4501 gaaaaaaaga cgtatcaaga atatttaaac aaattagtcg ctcctttgat cagaggctat
     4561 tatgtggatg aagaatttga aaaaaggatc ttgaaaagag aatatcagca aagcacattt
     4621 gttaacaaag aatttgcttt tcctcacaca accaaccagc tatctcaaca cattgtttta
     4681 tcagtagggc taataactga tcaaggttta gcagaaacag gaggaaatgg agttattttt
     4741 ttatttgcca tacctgaaga aatgacggct gaagttgaga atgaattatt tgagctatac
     4801 gatatcctat ttgaactcat aaaaatggga atagatgagt ttattgaatt agcaccgaca
     4861 caagaatcat ttttaaaact aatcaaaagt aaaggagaga gacttcatga atgatatgta
     4921 tctattcata gttttcgcag cttcagctta tgtgctgcaa gcagttatca gttggaagca
     4981 actaaagcat ttcaatcaaa catatagcga actgcgaaga aaaggaaaag tagcgattgg
     5041 taaacgacct gggaaaatcc gttcaggcac gattgttatg ctagctatag acgatcatga
     5101 tcaagtttta gaagtcagaa aaatgcaagg ggttacatct ttggcaaaat ttcattcatt
     5161 acctgtctat gcgggtgaag atatccatta catcgatcgt taccatccct cagttcgagc
     5221 ggaagatcgt ttgacacaag cagctttaga aaatgccaga gaagtatact tacgaataga
     5281 ggcaggaaat gaaaaaagtg aacaaccttc attgcctttt attgctatgc tcaataattt
     5341 tcggatatgg aaaagtacga tcaaagaaaa aaggagtgac aaacgatgga tatcataaca
     5401 aaatttgctg agggatttat gaggctgttc caaacaggtg cagaaacatt cataagctgg
     5461 atgacaggaa tcgtaccagt tgtcttgatg ctgatggttg caatgaatac gctgattgct
     5521 tttttaggtg aagaccgagt aacgaaagta gccaaattat cagcgaaaaa tccgcttact
     5581 cgttaccttg tgttaccgtt cttatctgct tttatgttgg gaaatcctat gtcatttaca
     5641 atggctcgtt tccttccaga atactacaaa ccaagctatt atgctgctca agctcaattt
     5701 tgtcatacga gcaatggtgt attcccccat attaatcctg gagaattgtt tgtatggatg
     5761 gggattgctc aagggatcga aaccttaggg ctaaattcga tggagctggc tatccgatac
     5821 ttgcttgtgg gactgctgat gaactttatt ggtggttgga taactgattt tactactgga
     5881 tttgtatgca gacagcaagg aatcgttctt agcaaaaaag tagagctttc agttgattag
     5941 agaaaggaga gttttaaatg tatggtgata ttaagatcgt aagaggaaac ggcgggtatg
     6001 gaggtccttt gattattagg ccgacaaaag ataaacacaa gttcatctat gtcacaggtg
     6061 gaggtgagag gccaatgatc gtagataaaa tagaagaact gacaggtatg gagtctatca
     6121 atgggttcaa aacatcaata cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag
     6181 ggactcttag atgtggaatt tatcctaaaa aagggatacc aaccatcaat atcgttccca
     6241 caggaaaaag tggcccatta gcacaatata tcactgaaga tatttatgtt tcagcagtag
     6301 ggttagaaca aatacagcca ctaatggaaa ctgatgataa caaagaaaca gtaaatactg
     6361 catctccaga taaaaagtac aaatatgata cttctaaaaa gataaccgag caaaaagcag
     6421 agcagcaaaa ctggatagca aaaattggaa tgggcgcggg aaaagtagtt gctacattca
     6481 atcaatcagc aagagaagca gtagatacga tgatccggac agtgatccct tttatggctt
     6541 tcgtttccct tttgattggt gtgatacaag gttctgggat cggtaatgta tttgctaaca
     6601 ttatggcacc tttagcagga aatgcagttg gattaacaat cattggcttt atttgttcac
     6661 tgccattttt atctcctttg cttggtccag gggcagttat tagccaagtg attggaacat
     6721 tgatcggtgt agagattggg aaaggaaata ttcctcccca attagcatta ccggcactat
     6781 ttgctatcaa tactcaaaat gcatgtgatt ttattccagt cggtctaggt ttggaagaag
     6841 caaaagctga gacagtagaa gtaggtgtac catcagttct ttattcaaga tttcttaacg
     6901 gcatcccacg agttatcatt gcttgggtag caagttttgg attatatagc tg
//
LOCUS       DASJPC010000113         6924 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJPC010000113 DASJPC010000000
VERSION     DASJPC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.113"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            94..1248
                     /locus_tag="U4A13_002469"
     CDS             94..1248
                     /locus_tag="U4A13_002469"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN1780732.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="U4A13_002470"
     CDS             complement(1270..2022)
                     /locus_tag="U4A13_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN1780733.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="U4A13_002471"
     CDS             complement(2386..5052)
                     /locus_tag="U4A13_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN1780734.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="U4A13_002472"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="U4A13_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN1780735.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2044 a         1421 c         1252 g         2207 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaa
//
LOCUS       DASJPC010000114         6815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJPC010000114 DASJPC010000000
VERSION     DASJPC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.114"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1336)
                     /locus_tag="U4A13_002473"
     CDS             complement(<1..1336)
                     /locus_tag="U4A13_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN1780736.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKN"
     gene            complement(1347..2015)
                     /locus_tag="U4A13_002474"
     CDS             complement(1347..2015)
                     /locus_tag="U4A13_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN1780737.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2145..2729)
                     /locus_tag="U4A13_002475"
     CDS             complement(2145..2729)
                     /locus_tag="U4A13_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1780738.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3060..3293)
                     /locus_tag="U4A13_002476"
     CDS             complement(3060..3293)
                     /locus_tag="U4A13_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN1780739.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3308..4258)
                     /locus_tag="U4A13_002477"
     CDS             complement(3308..4258)
                     /locus_tag="U4A13_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEN1780740.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4255..5097)
                     /locus_tag="U4A13_002478"
     CDS             complement(4255..5097)
                     /locus_tag="U4A13_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN1780741.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5099..5818)
                     /locus_tag="U4A13_002479"
     CDS             complement(5099..5818)
                     /locus_tag="U4A13_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN1780742.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6510..6668
                     /locus_tag="U4A13_002480"
     CDS             6510..6668
                     /locus_tag="U4A13_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780743.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
BASE COUNT         2327 a         1195 c          871 g         2422 t
ORIGIN      
        1 cattttttaa ataaataatt ctattgcatg ttttataaag aaattccgga tcatgagtag
       61 ctataactac tgcacccttt tcttttaagt ctttaataag gtcagcaatc ctttccatat
      121 taatataatc caacccactt gtaggttcat caaatagaaa taaattagtt tttgacaatg
      181 aagctataga aaccaataat ctttgttttt ctccaccgga taaatcaaac ggtaattgat
      241 ctttcatttt ataaagttcc atttttttta aaattttttc ttgctgatct ttactaacta
      301 tttcattccc cagggctaac tcgctcctca ctgattctgt gaaaaattgg taatccattt
      361 cttgcattac taaaaacggc gatgacaaat tttttttatt ttcgattatc tttcctttgg
      421 tagggcgaat aatcttcatg agtattctta gtaaggttgt tttccccaca ccgtttttcc
      481 ctaccaaacc aataacatct ccaactttga catcaaaatt tatgtctttt agaatatttt
      541 cataagtaat ttcttcaact tttaatacat ctgcacttac cttttcgata tagggaactt
      601 caatcgaact attaaaaatg tcaaatttcc ttaatgcgtt attgttttca gtttttaaca
      661 actgaatagt tgtagttttt acagttctat ttttaacaaa tagaacttga tcaattaatt
      721 ctttcaaata gtaaaaacga tgttcagcta taacaatagt aaatccttta ttttttaaat
      781 cacaaagtaa gtcagcaagt ttcttagtcg ctttgaagtc caaatttgaa gacggttcat
      841 ccaatataat taatttagag tttagcgata aactacatcc aatagaaatt ttctgccgtt
      901 caccacttga tagcgaaaaa atgtttttat ctaatatatt tgaaagatct aataattcag
      961 ctaattcatt gattctttta tccatatgct cttttgtaaa tccaaagttt tccatcgaaa
     1021 atactaactc actcgttgta tttgttgtaa agaattgtcc tctaggatct tgacttacgt
     1081 agccaatatt ttttgatata ctttcttgtg ttaaggattg tagcgagtta ttttcaaata
     1141 aaatttcccc aaccaatttt ccgtcataaa gtcttggaat aagtccattc attaacttta
     1201 atagtgtgga tttcccactc ccagaagaac cagtaatcaa tgtaagactg ccttttttta
     1261 ttgaaaaatt aaggtcaaca agcgcttgtt ccttaacatc cttatatgaa aaagaaacat
     1321 tgttaaattc aaacaattct ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg
     1381 gaaaacagga tgatatagtc taataagctc atgtttgttt tatagtaaga acttcttttc
     1441 acaggagatt ctatcccctt tgttattgca ttagcggtca tttcttcagc aatcatagtt
     1501 gcacgtataa gaacgggaac taacagaaat tccaaatatt gcagaggctg aaaaaaagac
     1561 aagcgaatgt ttctcatttt taaagattct ttaatcattc ttatttcaga acgataagtc
     1621 ggaaagaatc tgatcactac tgtaatactt aacattattt ttttcggtaa atgtattttt
     1681 tctaaagagg ataataattc attgctagta tagacactaa ccaacaacca tgagatcatt
     1741 acgaccggca acattcgaaa tacaatatat aaggatgtcg caaaaaatat agtaaaaaac
     1801 tctatcttga tagcatcaat aattttcatt actaaaaaca gaactgaata aatcaagatg
     1861 aaataaatac atgtctttat tttacgaaac atcaatacta aaactgtagc aataatgaaa
     1921 caccccactt gaaatatatt tccctttcct aaaaaaataa tgaccggaaa aaaaatattt
     1981 aaagtaatca aggttaacgg attgattttt gacactcttt cacctccaaa aaataagatc
     2041 gcactgttat cttaagctga taaaaatgca ccgttattca gataacagtg cgatcttatc
     2101 aaaaagcata actgagaaca attctcaatg caagcttttt ttaattaact cagtaattca
     2161 atccacccag gaacagaaaa ttgatacaat cttctggtta atagcatagt cttttctttt
     2221 gaatagctat gcttcaatat atcatagaat gtagcgtaga atgagtaaat gtagaagtgg
     2281 atttcttctt cattaaagac taaattttga ttgatcattc cgatagtctt taacttatct
     2341 agacaacaga ctgtcttttt cgtcacaaat gatgtaattt tttcaacaat atcttgatat
     2401 ttggttccag ctgattggaa taaaatcaaa tcgaatatac gtttattctc aaaaatataa
     2461 ttaataaatg gatctaattc attgaagttc tctctccaac ttttctctat atctatattt
     2521 tctaagttta agaggtattt ggtaaactct tcattataca tattaataag cgcattaata
     2581 tgcggctgaa taatttcttc aaataacgct tccttagaag taaaatgttt ataaaaagca
     2641 cccgtagtta cgcctgcggt caggcacagt tgccttatct tagtttgctg aaatccttct
     2701 ttttcaaaat ggcttagtcc actttctatt aacttttggt gagttataat ataatctctg
     2761 gtttctatgg gtacttcctc ctaataagat gaattaactt tattatctta cacaggactt
     2821 gaagtttcaa tcgttaacgt ttattactga actcattcct tagagctaaa ctaatgagaa
     2881 agagcagaat aactgataca aaaagcaatc ctacaagaat taatagactc atctaaatca
     2941 ttcacctttt ctaaaataat ccgagaaaga tttaaaaaaa ttatatccag ctattaggat
     3001 aagtcctatc ataatcactc caatagccat tttcttatcc tccctaaaaa taaagtcttt
     3061 tacttaaaac cttttttgta atcgtcaata aattttttcc ctttatcagt taattgaaat
     3121 cctatatcta aatagtcctc atatctaagt ttagccagca tctcacttac agatgctgga
     3181 ctaacactta gaacttttgc taaatcatta ttttttaatt tcttttttct ttttgataat
     3241 tcctgaattg ctagtaaata aattagtata gtagtcgttt taatttgtac catacctacc
     3301 ctccaaatta tttcgaaagg ccttcagcaa ttttatccag gttccatttc atcatcgcat
     3361 agtaactatc accatcttgt cctttttctg caattgaatc agtaaaaatc gttgaataga
     3421 taggaatatt ggtatctttt gatactgttt tcattggtct atcatccaca ctgctttcta
     3481 cgaataagga gggaactttt gttgtgcgta atttttcaac taagtgtttt atttgatctg
     3541 gtgttccttc ttcttcagtg ttgatttccc aaatgtaagc agaaggcaca ttatacgctt
     3601 tcgagaaata tttaaagcat ccttcacttg taacaatcat ttttttatca ttcggaattg
     3661 aagcaaattt agatttagct tctttgtcta gagaatccaa tttttcaata tacttatcta
     3721 gattttcttt atagaatttt ttattatcag gatctttttc cgctaactgt ttttcaatat
     3781 ttttagcgta aataataccg ttttctaaat ttaaccaagc atgaggatct tccttccctt
     3841 tctcactctg accctctaag taaataacat ctatgccatc acttgctgca aaatagtctt
     3901 tgttttcctc tttgttcgca tttttaacta attttgtaaa ccaagcattt cctccagttt
     3961 ccaagttaac accgttataa aaaatcaaat ctgcttttga agttttttga acatcttcag
     4021 gcaaaggttc atattcgtgg ggatctttcc caataggtac gatactgtgt aaatcaattt
     4081 tatcttttgc tatattttcg gtaatatctg ctaaaatcga attagtaact actactttta
     4141 atttttcgtt cgaagccact ttatctttgt ctttactatt tattgaacat gccccgaata
     4201 taaaaagagc aatcaaaatc cccagacttc ccagtactgc tttccatttt ctcatcataa
     4261 cgatctctcc ttctttctta aaaatttttg ttttggtgag attagaaaac taattacaaa
     4321 taagaatgcc gctgttaaaa caatacttga tcctgctgct atgttaaaac tataaccaat
     4381 aaacaatcca agcaatgatg aacttgctcc gactgttgca gaaataaata tcatagtttt
     4441 caagctattt gcatataaat atgctgtagc tgctggtgta attaacattg caacaattaa
     4501 tatagttccc acactttgca tagctgttac tgctactaaa gttaatagaa ccatcaatag
     4561 ataatgataa aaactgacat tcatccctat tgcttttgcc attaatggat caaaagaagt
     4621 gattaataat tctttaaaaa atagaactac tataaccaac actaaaaccg aaacgccaat
     4681 tgtaatccac ttatcaatat cttgtacagc taaaatattt ccaaataaaa tatgaaataa
     4741 atccgttgag ctatttgcaa ctcctataag gataactcct agtgctaaaa aagaactgaa
     4801 agtaatacca attgctgtat ctccttttat tacactattg ctttttataa aagtaataat
     4861 gatagaagct aatagaccaa atacaatcgc accaataaaa aaattaatac ctaaaatata
     4921 agaaattgct actcctggca aaacagcatg agaaatagca tcacccataa gagacattcc
     4981 tcttaaaata ataaaacaac ctactgttcc agaaacaatc cctataacta cagatgtaat
     5041 tagagcattc tgtaaaaagt gaaagtcata cagaccatta ataaaatttt gaagcatatt
     5101 attccccctc tccaataaat atagtatctc cataggcttt ttttaaattt tcttcattaa
     5161 atacagattc tacacttccg tgagcaatta gatttcgatt caaaagaata atattatcaa
     5221 aatacttctt taccttgctt aaatcatgat gcacaattag cactgttttt ccctgatgtt
     5281 tcaatttttt taacgtatcc ataattattc gttcactaac taagtctatc cctacaaatg
     5341 gttcatctaa aaatatgaaa tccgcatttt gagcaagaca tcttgctaaa agtacacgtt
     5401 gaaattgtcc tccagataat tctcctattt gatgattaga atattttccc atatcgacct
     5461 tttccaaagc ctttgaaact ctttgccatt ctgcattttt aattctttga aaaactttca
     5521 ttccggcata tgttcccata gatacacatt cttttacttt tattggaaaa gtaaaatcaa
     5581 tatcagcttt ttgctctaca tatgcaatct tcaccagttt tttattgata tcttcattat
     5641 taatggagac ctctccctga tgagggataa tatttaacat tgctttaagc aatgtggatt
     5701 ttccagcacc atttggacca ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa
     5761 tctcgttcaa tgctaaaaaa tcgttataac aaacattaag ctttttcact tctaacataa
     5821 ctttctcctt ctaaatgata atgattatca cgatcatatg gtatacaaaa attatatttt
     5881 agtcaaccct aaattttata gtttagattt ttttctgttg ctgaagatta gaatgacaaa
     5941 actatttgaa actacgcgta tagtaagtag ctttcaaaat atataaaaaa ggagcgttct
     6001 caaatgagaa catgctccta aaactacttt attggcgtat ggaaaaatta tacttgaagt
     6061 ggatagcggg ttgaataagt tgataagcat caaaacattt ataaaaatta ttcaagacta
     6121 tgtctgttcc atatacaaat atgaaaatca gtaaactcca tttaactttt ttcgtagtgt
     6181 gtctttttat acatgtttat cgttattaat agtgaatagg aaaaaaacta tttttgatca
     6241 ttgacaatta aatacaattt ttgaataacg atccctgcta ttcgagctag aaactcaagt
     6301 acgccatgac ctgattagcg acaaatactt agagatttct ttttgttctg ggaaaacaaa
     6361 atgccatgac aatagcagca aataatgata cttccgtaag gctcgccgta aactggggag
     6421 aggtgaaatt cctatgtgct tacccaaagc aattcaaaaa tttcataact actattttag
     6481 gagatgattt tatagaatcc agagtctacc tgaataaaaa acaaacctgt gcatttctag
     6541 gaatcgataa taatactcta gataagtgga ttcgtatata taatttccca gtaataagag
     6601 ttggtggtgt agttcgcttt gacgtagaag atattaaaga ctttatgaat acatttaaaa
     6661 taacttgaaa ttttcttctc gccgcatata ctaaaattag tgaaatatac tgccttactt
     6721 tttttaagga gtgaggattt tgcctacaaa gctttcaaat gggaaatata aaactaatct
     6781 gagatatcca aaaaggttta aagaaataac aggaa
//
LOCUS       DASJPC010000115         6643 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJPC010000115 DASJPC010000000
VERSION     DASJPC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.115"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..859)
                     /locus_tag="U4A13_002481"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="U4A13_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="U4A13_002482"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="U4A13_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN1780744.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="U4A13_002483"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="U4A13_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="U4A13_002484"
     CDS             complement(2143..2373)
                     /locus_tag="U4A13_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEN1780745.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="U4A13_002485"
     CDS             complement(2603..3421)
                     /locus_tag="U4A13_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1780746.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="U4A13_002486"
     CDS             3582..4271
                     /locus_tag="U4A13_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN1780747.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="U4A13_002487"
     CDS             4285..5787
                     /locus_tag="U4A13_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1780748.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="U4A13_002488"
     CDS             5800..6276
                     /locus_tag="U4A13_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN1780749.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="U4A13_002489"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="U4A13_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DASJPC010000116         6588 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJPC010000116 DASJPC010000000
VERSION     DASJPC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.116"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            23..1033
                     /locus_tag="U4A13_002490"
     CDS             23..1033
                     /locus_tag="U4A13_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1780750.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1102..2046)
                     /locus_tag="U4A13_002491"
     CDS             complement(1102..2046)
                     /locus_tag="U4A13_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN1780751.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(2030..4750)
                     /locus_tag="U4A13_002492"
     CDS             complement(2030..4750)
                     /locus_tag="U4A13_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1780752.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(4747..5967)
                     /locus_tag="U4A13_002493"
     CDS             complement(4747..5967)
                     /locus_tag="U4A13_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN1780753.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(6248..>6588)
                     /locus_tag="U4A13_002494"
     CDS             complement(6248..>6588)
                     /locus_tag="U4A13_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEN1780754.1"
                     /translation="IPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFD
                     RLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGV
                     SQAFERILTI"
BASE COUNT         1928 a         1266 c         1077 g         2317 t
ORIGIN      
        1 aattacagga gtgcaaattt taatgaaaaa aaaatcaatt attttggcag ctactagtgc
       61 gttggctgtt ttaactctag cagcttgttc aggcgatact aataaagata tcgcaacgat
      121 gaaaggcgga acgatcactg tatctgattt ctatgatgaa gcaaaacttg aatcatcgaa
      181 ccaatcctta gtacaacgga tgatcattta taaagtattc aataacaaat atggcgataa
      241 agtaaccgat aagcaagtgg atgcagaata cgacaaacaa gcgaaatcat taggtgatac
      301 ttttgaatca caattagaag ctgctggata tacgaaagat acttataaag aatatattcg
      361 taacaattta gcttttgaag ctggcttgaa agctcacgta gatattacag atgacgattt
      421 gaaaactgct tggaaatctt tccatccaga agtagaagca caaatcatca aattatcaag
      481 tgaagatgaa gcaaaagatg tgaaaaaatc agcggatgat ggagatgatt tctctaaatt
      541 agccaaagac aaatcaactg atacagagac aaaagaagat ggcggtaaag ttaaattcga
      601 ctcaacgaca acaactattc ctgcagaagt aaaagaagca gctttcaaat tgaaagacgg
      661 tgagatctcc gatgttatta ctacaacaaa tcctacttct tatgcgacag aatactacgt
      721 tgtcaaaatg gtgaaaaacc aaaataaagg caatgacatg gataaataca aagatcaact
      781 aaaagatatc gctactgaaa ctaaactaag tgacaatgca ttcacaacta aagtaatcgg
      841 tgaagaatta aaagatgcaa atgtaaaaat caaagatgat gcctttgaaa atgtgttgag
      901 tgcattcaca acaacttcat caagtacgaa agactcaagt gaaactacag cttcaacaaa
      961 gagttctgac acaaaatcaa ctgattcaac aaaagaatct tcaactgaag aaacaactga
     1021 ttcatcaaaa taaaagaaaa caaaaaaagc gaagacttat gtctcgcttt ttttgttttg
     1081 aatgaaaaaa ataaattttt gctataaatc ttttggtaca tagaaacttc ggttgtccat
     1141 accgaaaatc cgatcggtgt attttcctgg ttctgtttga cgaatcgaag tcaacatcat
     1201 ctgcatcgaa gcatcgatat tgtcgatttg atgtaatatt tctgcttcca ttattcttgg
     1261 acgtacaggt gagccatatt ctaataagcc atggtgtgct aaaaccatat gacgtaatac
     1321 aagaacatct tcgtcagctt cgctgatttt caacgcaaga caggctttag ttatttcttc
     1381 gtcaatcaat accagatgac cgatcaaatt tcctgcaagc gtatattctg tcgacatagg
     1441 accggaaagt tcaatcacct ttcctaaatc atgcaaaatg ataccagcat acaacaatgc
     1501 tcgattcagc tcaggatatt catcggcgat tgatttccct aagtgaagca ttgtcaaagt
     1561 atgataagct aatccactgg caaatgcatg atggtttctt tttgctgccg gaaactcaaa
     1621 aaattgtttt tgatatttat taagtagata gcgaacaata cgattccaat gcgcattggt
     1681 gatctcaaac aacaattgat tgatttcttc ttccatttct tctttcttca aaggggctct
     1741 ttccatgtat aaactaggct ctcctggctc atctaccctt gttaagcgca tatgaagaat
     1801 ctttacttga gggtttcctt gataaacctc tcttttaccg tttagaaaaa cgactgtgcc
     1861 ggctgtaaat ttattgattt cctcctccga agcatcccag tatttaccat cgatagtacc
     1921 tgaagtatct tggaaagtaa aagcaataaa tttttttccg tttttagcga ttcttacatc
     1981 tgcacttttg atcaggacaa attgttcaaa ctgctcatcg attactaaat cacgaatttt
     2041 tttcattatt tgctccttct aattgtttta cgatttggtt catctcatgg taatagctta
     2101 ccatttcttt atcagaagaa aaacaaatta tttgatactt ttctccaaat ttagctaaaa
     2161 gttcagctaa ttgatacttt cgctgatgat cataatgaag ccagccatca tcaatgatga
     2221 tcgggcatat cgaacggtta gcttctaatg agaggtaagc aaatctcatc gccataatca
     2281 gctgatcttt cgtacctgta gataactcat agatgggaaa ttctgtatat ccgtctgtta
     2341 aagtcagtgt atcatttttc agtaatatct tctgataatt tccttttgtt aaaataccta
     2401 aataatcacc tgctgctctt aacaactggg gtaattgtct ttcactcatc tctgttgaaa
     2461 gatcacctaa gaatgtcccg gcgatttcaa gtgctcccca ttcctccagt aattcctgta
     2521 attttgcttt ttgccggcta gcttgttgat aaagttcgtc caatgtgccg tctctttgca
     2581 actcttctac ttgaagttgg agtcgttgcc gattttcact tgcttctttt tcttcttgct
     2641 ggagttggga ttgcttttct ttcagttggc gcagtcttac agataatttc tcttttgtca
     2701 gctcttcagg aaatagtgga gtaagcattt ccgataattc attgtatctt ttcatctgct
     2761 gttcggcttg tttggctttt tgtaaaaaga caggtacttc cgaaggataa gataagcctg
     2821 cttgagtcaa ttgttcgctg tattcttgta tccatacttt ttgtttcttt ttcaattgat
     2881 tgatttcttg cttcaataaa gtattttgct gatagcttct ggcaaatttc atctgttcca
     2941 tctcttgtat aaaacgggaa agcaatacca ttttttctga aattgtctta tcttgtatcg
     3001 gtaaccattc tttaacaaac tcaaattttt ggtcgatttg tcgtagtttt tcttgtattt
     3061 cctgttctct tttatgtttc cgctcaaata cgccatataa tttctgataa tctatgactt
     3121 cctgtccata ttctttcatg agtttgagcg tatccatctt tcctaagtgc cgatgatccg
     3181 caaaacgata tacttcttgc tgaagaatct gtttttcttc tccagttctc ttttgttttt
     3241 cttctgcttc agctaattga gctaaggcta gatcaagttg tcctagtttt tcttgccaca
     3301 ttccttttat ctcagagaaa tcatttttag ttttaatttt tggttgcctt ttgtagaaga
     3361 aatagccaat catgcctgca ccaattagta aaaggatcac tttcaagggc attggtacaa
     3421 atatacctgc aatgattatt gctccgccag ccatcagcat atacggttta gatgaagaga
     3481 ctggtgctat ctctgactga ttttgtaata atgctggata ggtctcttcc aagtcattca
     3541 cttctgcttc aatcatctct tgctgttctt tagctatttg gagacgcaca gaaatctggc
     3601 tttctagatc agcaatcctt ctttcttcag ccagaagatg cttccattct tcttcatata
     3661 aaaattcttc cggtaacgga tttttctgcc aatgccacct ttgttccaaa atcttcaatt
     3721 ctgaatttgt ttctctagtc tcaatttgta atctttgcca ttcttcgttt aattgttccg
     3781 ctgtaaattg aagttgctgg agatctttta gttcctgctc ctgctctaaa taaaaataat
     3841 atctggctga ctgctgatcc atcccgctat gcttttccag tgttgcttct aattcttcca
     3901 aacgctcatt tgtttgctga taccgtttgg agaaagactc cagatctaaa agaagttgct
     3961 catcaggaag acctgtagtg ctgttttttt tgagagattg ccattcttca tataaagaaa
     4021 aattaagttc ttgctgtctg gctaatgaaa attgtttgtt cgcctctttt aattgtgcca
     4081 ataactgctg ctgttgcgac tcgtattcct gttcttgttg gatcaacttg ccaaaataag
     4141 cttcctgttc ttgtttttga tgaatttttt cttgcagctc tttccattca tttaattttt
     4201 gattgattag taatttttgg ccttttttct tgaataattg ttgtgctttc tgataatatt
     4261 cttgccgctt ttggaatact tgagaactgc ccgtgatgcc taaagaaatc agcgcatcat
     4321 gtagctcttt ttctctcaaa tgatctagtt ccatcaactg ctcttgctgg aaagtaaata
     4381 cctgctggaa taactctttc gtcaaaggtg cgatcagttt ttctagtaac tcatcacttc
     4441 ccacttgatc ccccagccac accttcgatt ttccgcgatt ttgctgcttg tagcgctcga
     4501 cagcaaattc accatataca ggatgtttaa gccatatttt gccgccatag gctgagccgt
     4561 cttttggtgt ataatccttt tttttacggc cttttgaagg gaaaccaaat aatattgctt
     4621 gaataaattg ataaatcgtt gattttcctg cttcgtttcc cccatagatc agctgattgc
     4681 ctgaataaaa gtcaaaactt ttttgacgcc attttccaaa agcagcaatc tcgatcctta
     4741 aaatcttcat tcctgatcct cctcaaataa aaaatcttga ttaagcagct gcttggcttt
     4801 ttcaagtgtt tcttcttgat attccggcag ttcgctcaat attctagccg cttcttgatg
     4861 actatatact tcatttaaaa tttgttggaa atcttgaggt gtctgatagt attgaaataa
     4921 ctgttccatc aatttagcag aagcagcaag tggggtcttc gtataaaaag tattctctat
     4981 gagagaaata cgccaaataa agaaatcatt tatcctatct gagaattcct ctaacaaata
     5041 gtctaatagc tctcctgatt gtatccgttc caaaacatct gtattgagat ggtcataatc
     5101 ttttaatttt atctcaatta aactaaatcc atcctctact tttgaaagct gctgacgaat
     5161 gtgaacaatg acctcttttg tcgttcttgc tttcctcaat gatatttcct ttgtcttcca
     5221 atagacctct gctactttaa tcggatgtat ttggcaactt cctttactta attcaactag
     5281 tagaatagat gtagaagctt gttccttttt agtatgacct tgaggcgcac ctggataaac
     5341 aattgtttgc ttttcattta agaccgttgg cacatggata tgccctaaag cccaataatc
     5401 gtatcccttt tcttgcatct ttgagggttg gaaaggagca tagtttcctt tttgagagac
     5461 gccaggttcc ccgtggtaca acccaatatg gtaatctgtg agttctctag aaggaaattc
     5521 catcactttg tctttttgaa tccacggttg acgataacta aatccactga gagagacagt
     5581 ttctccactt tttatcgtta ttttttttgt ctctaccgtt tcggatgtaa ataaatggac
     5641 atttttagga aaggagaacc aataccgctc tttttgatag aaatcatgat ttccgaatat
     5701 tataaataca ggtatatggt tcttttctaa ccgtttcatc tgttctgaaa aatgtttctg
     5761 tatttttaat gaaggacgat tttgatgaaa attatctcct gcaagtaaaa caaaatcaac
     5821 gttatttatt attgcctgtt caatgatatt tgataaaacg gtcagattcg tcttcaatag
     5881 tttttcctgt acagttttat caagagttgt cagtccttca aatgaccgat ccatatgtaa
     5941 atctgccgca tggataaatt tcaccattaa aaatttcctc ctttttttct tttatcatac
     6001 cattttcaat gttttttttc atcaataacc gaaaatttgt tcgtattatg caaagaaatt
     6061 acgataaatg gccgacttca aaaaataagt ttattcgaaa aaaagattgt gacatgattt
     6121 ttgtcacaat cctaaatatc tatgttatat tcgcttcgtt tgacactgaa gaagtactat
     6181 ttgtactgga atttatgctc catttttccg tatttagaat tctatgttct atactacctt
     6241 aattagttta tatggtaaga attctttcaa atgcttgaga tactccgtct ttatcatttg
     6301 aaacagtgac ataatcagct tttttcatga tttcttgatg actatttccc attgcaaaac
     6361 taatatctgc actttcaaac atagataagt catttaaact atctccgatc gcgattctca
     6421 aaggtttctt taaattgtag attgattcca aacgatcaaa caccacacct ttagatacat
     6481 tttgaggtac gatttcaata tttacaggtg ccgattcagt aacagacaca tttagcatct
     6541 ttttcaaatg gttaaatata ttagctttca attgacgatt aggaataa
//
LOCUS       DASJPC010000117         6570 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJPC010000117 DASJPC010000000
VERSION     DASJPC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.117"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..579
                     /locus_tag="U4A13_002495"
     CDS             127..579
                     /locus_tag="U4A13_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780755.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="U4A13_002496"
     CDS             593..1282
                     /locus_tag="U4A13_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780756.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="U4A13_002497"
     CDS             1407..3758
                     /locus_tag="U4A13_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN1780757.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="U4A13_002498"
     CDS             3838..4233
                     /locus_tag="U4A13_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780758.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="U4A13_002499"
     CDS             4558..5175
                     /locus_tag="U4A13_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1780759.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="U4A13_002500"
     CDS             5216..5515
                     /locus_tag="U4A13_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEN1780760.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="U4A13_002501"
     CDS             5549..5716
                     /locus_tag="U4A13_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1780761.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="U4A13_002502"
     CDS             complement(5761..6366)
                     /locus_tag="U4A13_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1780762.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DASJPC010000118         6564 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJPC010000118 DASJPC010000000
VERSION     DASJPC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.118"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(7..1164)
                     /locus_tag="U4A13_002503"
     CDS             complement(7..1164)
                     /locus_tag="U4A13_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780763.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="U4A13_002504"
     CDS             complement(1167..1523)
                     /locus_tag="U4A13_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780764.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="U4A13_002505"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="U4A13_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN1780765.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="U4A13_002506"
     CDS             complement(2991..3830)
                     /locus_tag="U4A13_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN1780766.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="U4A13_002507"
     CDS             4015..4902
                     /locus_tag="U4A13_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN1780767.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="U4A13_002508"
     CDS             complement(5486..6181)
                     /locus_tag="U4A13_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEN1780768.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="U4A13_002509"
     CDS             complement(6165..6563)
                     /locus_tag="U4A13_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN1780769.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1300 c          966 g         2233 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catc
//
LOCUS       DASJPC010000119         6307 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJPC010000119 DASJPC010000000
VERSION     DASJPC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.121"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            79..612
                     /locus_tag="U4A13_002510"
     CDS             79..612
                     /locus_tag="U4A13_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780770.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            624..827
                     /locus_tag="U4A13_002511"
     CDS             624..827
                     /locus_tag="U4A13_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780771.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            1149..1529
                     /locus_tag="U4A13_002512"
     CDS             1149..1529
                     /locus_tag="U4A13_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1780772.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            1511..2425
                     /locus_tag="U4A13_002513"
     CDS             1511..2425
                     /locus_tag="U4A13_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1780773.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            2385..2663
                     /locus_tag="U4A13_002514"
     CDS             2385..2663
                     /locus_tag="U4A13_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780774.1"
                     /translation="MNEEQTTNKNYRSKTLKDIENEIKRQRAFLAPYTQERIQTTMDS
                     QVKKLHQALLDQRNQEQKKALIQRSMDILAPLCCLVLTLLAFWLVWGE"
     gene            2670..3359
                     /locus_tag="U4A13_002515"
     CDS             2670..3359
                     /locus_tag="U4A13_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780775.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(3480..3566)
                     /locus_tag="U4A13_002516"
     CDS             complement(3480..3566)
                     /locus_tag="U4A13_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1780776.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            4242..5192
                     /locus_tag="U4A13_002517"
     CDS             4242..5192
                     /locus_tag="U4A13_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEN1780777.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            5213..5746
                     /locus_tag="U4A13_002518"
     CDS             5213..5746
                     /locus_tag="U4A13_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1780778.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5762..5932
                     /locus_tag="U4A13_002519"
     CDS             5762..5932
                     /locus_tag="U4A13_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780779.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
BASE COUNT         2325 a         1103 c         1175 g         1704 t
ORIGIN      
        1 caagcctata ccttttgttt tatgattttc acaaagaaaa cgttttctat atatttttct
       61 gtatcaggag gaaattctat gaaaaaattt actttagggg tagctttgtt aactattggg
      121 gcagttgttt taccgtcaac tactatagtt tatgcttcag aaaattccca agtggtatca
      181 ttaaacgaaa acaatgctac tgaaaatgta gttattgagt acactgaaga tggtatggtc
      241 gtaactaaat atgaggatgg ttatagtgcg gaagattttg caaattcttc agatatcatg
      301 acaagagctg cagttgcaag atggggcaaa aaggaatata caaatattgc tgtaaccact
      361 ggagtagctg ctaatgctat taatgctgcc ttttgggcag gagggaaagc tagcacttct
      421 atgtttggta tcccaggttg ggcaattggt gctttgttaa caggtgcaag ttggactaaa
      481 aaaggtgctt ctccagggaa tgcagttgca aaagaatggg acaaaaatgg taatggctgg
      541 attggatttt attaccaaag gggatatgat gcagctggaa gagttgtagc tactagatat
      601 tcaacgttat aagttaagga gtgatgtttt tgatatctaa atcattaaaa tcattaaaaa
      661 catttactaa aaagaatccg caaatagcta ctattcgtat ttttatattt gctatagctg
      721 ttgctataat tgttaatatg ctaagcggag ggcaattata caaatctggt ttatttttag
      781 tgttaatctt agttgtctta gacataataa agtctaaaaa aaaataaatc gatacatttt
      841 agaagaatag ggttgtttga atattacaaa gcaacaattt caccttcggt gaaataagcg
      901 aaactttaca aaaagtggct agcaaatgct agcctttttt tgtattgtgg agcgtggatt
      961 ttgaggggga ttcaaggggg agcttgcgct cctttgacaa gcggttattt gcaccaaata
     1021 cggaggtatt tggctagcaa atatagagct tgccaaaccc ttttagtgag ctttactcat
     1081 tgtacctttt taggtacaat ggtattgcga acgatacgtc cgtaggacgt actgaaaggg
     1141 tgggacttat gagcgaacaa catcaaaatt tggctagcga tcattctaaa aaatatacct
     1201 atcgatccga gccaaaacaa atcagttttc gagtgagcga atccgaattt gcaaagctga
     1261 agcagtcagc tgaagctttg caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa
     1321 acgcgcgtct ggtgacaccg aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt
     1381 tagccaaagc tggagggaat atcaatcaaa ttgccaagtg gtgcaataca caccaacacg
     1441 acgttgctcc tgatgatgct caacgtttat ccgaaaacct aaaaatcatg caaaaggagc
     1501 tacaaaagat atggcaacag ttaaagtaag tcgaaccact tcatgtagcc gagcaatcaa
     1561 ttacgcagaa ccacgagcaa ccgtcaaaac aggaataaac tgtgacattg attatgccaa
     1621 aagtgaaatg aaacaaatac gcacgcttta tggaaaagac gatcgtgtac aagcacactt
     1681 attgattcag tcctttcgac caggagaaat taccgctgaa aaagccaatc aactaggggc
     1741 agagtatgcg aaaaaaatag cccctaacca ccaaatcgcg atttacacac atacagacaa
     1801 agaccatatt cataatcaca ttgtcattaa ctccgttaat cttgaaactg ggaaaaaatt
     1861 tcaagcccat gggcaagcat ttttaaacaa atgttatgac ctaaatgacg agatttgtct
     1921 tgaacatgac ttaacaatca cggaacgagg aaagaaaacc gaaaaacgaa ccatgtctga
     1981 aattaaatta aaagaaaaaa atgaaccggt ttggaaagac gaaattcggt ttgcgattga
     2041 tcaaacaatg aaaaacaaaa aaactcgaac gtatacccaa ttttgtgatt cacttaaaac
     2101 attcggcatt cattgcttga atcgcggaaa aaattttact tacgaattgg tccacaaaaa
     2161 gaaaaaagta cgatccaaca aactaggaaa ggattacgaa aaggagacga ttttacatga
     2221 gctggacaga cgagaacgaa cctacaatca acaacgaacc aacgagctac gaagagctcg
     2281 agctagcatt cgcgaaactg cagaagaagt acgaagagaa caacgaactt ttacgcctga
     2341 acaatcggaa attagaccga ctcgaagaaa ccatgaacaa cacgttgaac gaggaacaga
     2401 ccaccaacaa gaattatcgc tctaaaacgt taaaagacat cgaaaacgaa ataaagagac
     2461 aacgtgcctt tcttgcgcct tatacccaag aaaggataca aactacgatg gattcacaag
     2521 tcaaaaaact ccaccaagcg ctattagacc agcgaaacca agaacaaaaa aaggctttaa
     2581 ttcaacgatc gatggatatt cttgccccct tatgttgctt agtactaaca ttattagcct
     2641 tttggttggt atggggagaa taaacagaca tggcattcta tttaccctat ctactcattt
     2701 ctgccagtat tttaggaatt atttggctta gctataaggc ttatcaaaca agatattcat
     2761 taaaaggctc taaaattcga tttaagcgat ttttattggt cggggttata tttgccttat
     2821 caatcgtttt aagtggttta ttcggcgttt tagagggtaa taaacgtgtt tcaagaagca
     2881 ttttattagg ggatatcact caaaagtatg aagcagcatg tgataaaaag aaaaaagagc
     2941 aagcattagc acaaaaaatg gaggaattta ccgcttgtta tgaggacatg aacaatattt
     3001 ttgtggatca agagaaacgt ttaacagata aaaacatgaa aaaactcact cgtttttatc
     3061 aaaacttacc agaaaaacag caaaaagagg tacaagaaaa atatgagcaa gtaaaaaaag
     3121 acgtgcaata ttttaaagat acgcagacag aagaagcttg ttgtaactta tttagcgata
     3181 caatcccttt ttcaaccagt gaacaagaac gaaaagaacg gcaacaatcg gtcacatacg
     3241 aacgttatga aacactgctt cagcaagcaa caaacataca aaatccaacg aaaaaagaaa
     3301 cggcattaaa ttatctagaa agtgtcaagg aatggttgga tcagcaacaa caaaattgaa
     3361 tttgattagc aatcatgtta aactgaacct gcaaatgatc aaacattaga tacacgaaag
     3421 ccccagagag gtcggattct ctggggcttt ttttcaggtt gatgtcgcct ttattgattt
     3481 tacttcttgt ttcggttatt caaccaataa ctgaataaag agacacaaac ttctacaaga
     3541 agggggcaaa tgaaccgttc aaacattaga tacacctcct tccagtcgca agactgtcgg
     3601 ataggcgaca agttattata ccttacaaaa taaaatctgt gtggtgtttt taagtatccc
     3661 ttattttcgg gttgttaggg cttgccctga ccgcctgtaa ggcgctttaa gaacaagata
     3721 aggtttttta cccaagactg tcttaaaacg acttagacga gctatcagag gcttccccca
     3781 cctccgttac tgacttgttt ttagcaaagg agcctttagg cgactgcgca gtacaatgtg
     3841 agagcatagc gatcacactt ttttagcgac cgtagggagc aaggttttga attggcagtt
     3901 ttgaaaaatg cgcgaacctg ttggtagaaa aaagaagtgg tttttaggcg ggaaattttg
     3961 tcaccctctt ttataatctc tttttaaacc tttttttaaa cctctttacc gtcggctgtg
     4021 tccttactcc ttcaatggat acagcgtttt ataaactaca ttttgtctgc ttttaaacta
     4081 cattttgtct gcttttaaac tacattttgt ctgcttttaa actacatttt gtctgctttt
     4141 aaactctttt tttgcactca cgtatgttat aatatgcatt atcacagaca aaacgttgtt
     4201 tataacgtct gtatccattg aaggagtaag gaggaatatc tttggcaaat gaaatcgtga
     4261 aataccatca tgaattaaat accattccgt tgcggaaatt tacaccagtt gaaatgaatc
     4321 tatttttctc tattgtgtct agaatgcgcg aaaaaggcga taaaacagtc cgtcttactt
     4381 ttgaccaact aaaagattta agtaattata aagcaactgc taataatcgt ttcattaatg
     4441 atttagaaat gacttatgat aaattaatgg atttacgttt tggtaaaaga agtgctaatg
     4501 ggctcgatat agatagattc gttatgttta gtgagtttaa aatacgtgga actgctaaag
     4561 atccatatgt agacatacaa gtttacgaaa aagctttacc tttactaaac aatttagatg
     4621 agtgggttcg ctattcactc caacaattta acgaattaca aagtagctat gcaaagacta
     4681 tgtttcgatt actaaaacaa tatcgaacta aaggttttgc ttatttttct aaagaggatt
     4741 ttcatgaact attagatatt ccaaaaagtt actggaacaa ccaagcaaac gttgataatc
     4801 gtgttataaa gcctatacga caggaattaa ccgcaatctt caaaggatta tcaatcaaaa
     4861 agaaatacgg aaaagggcga ggaaaacctg tcgttggcta tcaatttacg tttaaaccag
     4921 aaatcaaaaa agcagatgat tttaataaac atgcaccaac caaagaagaa caaaataaag
     4981 cattggcttt agctgataaa caaaaagaat acgaagaacc tgctacaaca attaacgaaa
     5041 aaatcaaaca gaagaaacga gaacttgaac tcctccaaca aatcaaacaa ctagaaaaag
     5101 agaaagaagc tcttgaaaaa caaacagagt ccgcactttc gaaaaaagag aaaaaaagtt
     5161 tattatctga tttagataat ttattccagt agcaacctag gaggcaacca gtatgcaacc
     5221 acaagaaacc tttacaataa aggaattgtc agaacttttt aagatgagta ggcaagcaat
     5281 atcgaaacat atccaaaaat tagattcttc catgatagct aaaaatgagc gtggttataa
     5341 ggttgtattg cgttctggag tattgcaact tgctcgcaac ttaggcaacc aaatgttgct
     5401 agataggttg cacgagtacg aacaagacaa cgataaaaat gttaatatac caagcaaaaa
     5461 cgtggttaca aacaagttgc tagatcagtt gcgtgaagaa aatgaatttt tacgagaaca
     5521 acttgccctc agagagcaaa ataattctca tatacaaaaa ctactcgacc agcaacaaca
     5581 actcacccta caagccaaca aacaaatcga aaaactgcaa gaaaaactac agttgactta
     5641 tacagaagaa gctccagaga atgactcaac gactctttct gaagcaacag aaacaataga
     5701 caaacaaatt gatactgaaa aaaaatggtg gcaactttgg aaataactgg agggaacatt
     5761 catgacaaga aaaaatcttc tattcggttc tattttacta ggaattctag gattcagtcc
     5821 gatgttattg tcagtgatca ataaagggaa taaacatggt gaattgaaat tgaatccaaa
     5881 ttattacaac agtcctaacg aagaattgtt tgtaaaagaa acagataaat aataaaaaag
     5941 actgctcttt cgagcagcca gttaaaaaat aatgagtgtt tattcttgaa aaagcaacgc
     6001 gcttatgaag cgctaccaaa aatttatcat attttattaa aaataacaac ccagtaaaaa
     6061 atgactggta aaaattcaca aagaaacgga aagaagcaac cgcagaaaat caagcttaaa
     6121 aagagctttt gacctagacc aatcgaaaat cacaaagtga gcgcctgtat ctattgctac
     6181 aggcgctact tggcatagtg tacattatgt caacaagaat ttaatataac atattttcct
     6241 aatgcattct aactatacca cattatcaaa aatataaatt atctttacta aataaaatat
     6301 tgacaag
//
LOCUS       DASJPC010000120         6303 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJPC010000120 DASJPC010000000
VERSION     DASJPC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.122"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(406..780)
                     /locus_tag="U4A13_002520"
     CDS             complement(406..780)
                     /locus_tag="U4A13_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN1780780.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="U4A13_002521"
     CDS             complement(803..2203)
                     /locus_tag="U4A13_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEN1780781.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="U4A13_002522"
     CDS             complement(2207..2617)
                     /locus_tag="U4A13_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1780782.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="U4A13_002523"
     CDS             complement(2635..3126)
                     /locus_tag="U4A13_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN1780783.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="U4A13_002524"
     CDS             complement(3136..3927)
                     /locus_tag="U4A13_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN1780784.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="U4A13_002525"
     CDS             complement(3920..4693)
                     /locus_tag="U4A13_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN1780785.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            complement(4849..>6303)
                     /locus_tag="U4A13_002526"
     CDS             complement(4849..>6303)
                     /locus_tag="U4A13_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1780786.1"
                     /translation="NHISIEESMSQLRDRILKECAGSSEYNYLESDEFIALRNYVVNK
                     ISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFRMKNDKQKLLANE
                     IWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITASAIARETNKLLNT
                     YVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDL
                     VILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSG
                     EGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGI
                     DCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTKLRFLDSKKVFNE
                     IEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHLKEGAQLFDTFLN
                     VSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
BASE COUNT         2029 a         1252 c          807 g         2215 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga ttaactcata ctctcctttg ggtatttcaa tacagtaaat
     4921 atctttgatt tctgaaaaaa aatcttttga tacatttaaa aaagtatcaa atagttgcgc
     4981 tccctctttc aaatgttcat ttgttattcc atcaccaatc ataattcgtt caatcattcc
     5041 agaaagatgt agaaatagat taattctcag aaaacttggg attttgacat cgtaatattt
     5101 ttcatacaaa tatagtactt tttctatctc attgaatact tttttggaat ctaaaaagcg
     5161 aagcttagta ctagcgcctt caatagtaaa gagtttaact aattcattta agcagaactc
     5221 acagctatcc gagtccatat acttatttaa tttttctaag gtttgattcc cattaattag
     5281 attttctaaa tttaaacaat cgattccttg aatgtaaaat tcagtggttg aaagaacaca
     5341 aattgtattc ttaaaaatca tagtatcttt ttcctttgct attttcttta atgcgttaaa
     5401 atcaaaaatc aaaatctcca cttgattatc gagataccgt tttaaaatat ccttaacttt
     5461 ttcagaaatc ccctcaccag agagacaaga tactattaca tttttctttt gtgataatcc
     5521 tttataatac tgaactttaa cttgaaacga atcaaaatcc atctggtaaa aatgactaat
     5581 tggctttttt tggcaaactt gaatcccaca ttcaatcgct aaagctgtag aaacattgtt
     5641 taaaattacc aaatcaccta ttacattgga ttcaattttt tcatacattt gttccaatga
     5701 tcccatatca actagcagaa tgagtccatt cgtcgtatct atacttttac aaaaattgtt
     5761 gattaggtta attaagtcac tagtctcacc ttcaattggc atatcgaaag cctcataaac
     5821 atatgtattc agcaacttat ttgtttccct tgcgatcgca gaagctgtta ttttaccgtg
     5881 cataactatc agacaatggg tttctggtat tttcacatag aacttcttta caagaaaaag
     5941 tatccatttc aacaaaagtt cttgtttact ttcttcgact tctaaactac tccaaatctc
     6001 atttgccaat aatttttgtt tatcattttt catacgaaat tttctgaact tagacagcaa
     6061 aaaatttctt gatgtttctt caattacatc aataaacatt ataaatagac tgatatccgc
     6121 caagaattca tctaataaac atagacctgt cgaatcaata attggtgata ttttattaac
     6181 aacatagttt cttaatgcta taaattcgtc actttctaaa taattatact cagaagaacc
     6241 tgcacactct ttaagtattc tatctcttaa ttgactcatg ctttcttcaa ttgaaatgtg
     6301 att
//
LOCUS       DASJPC010000121         6287 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJPC010000121 DASJPC010000000
VERSION     DASJPC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.123"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..177
                     /locus_tag="U4A13_002527"
     CDS             <1..177
                     /locus_tag="U4A13_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780787.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="U4A13_002528"
     CDS             190..690
                     /locus_tag="U4A13_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN1780788.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="U4A13_002529"
     CDS             787..2634
                     /locus_tag="U4A13_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEN1780789.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="U4A13_002530"
     CDS             2637..3533
                     /locus_tag="U4A13_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1780790.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="U4A13_002531"
     CDS             3583..3972
                     /locus_tag="U4A13_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN1780791.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="U4A13_002532"
     CDS             3959..>6287
                     /locus_tag="U4A13_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1780792.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DASJPC010000122         6255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJPC010000122 DASJPC010000000
VERSION     DASJPC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.124"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="U4A13_002533"
     CDS             complement(128..676)
                     /gene="aac(6')"
                     /locus_tag="U4A13_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1780793.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1229..2332
                     /locus_tag="U4A13_002534"
     CDS             1229..2332
                     /locus_tag="U4A13_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1780794.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="U4A13_002535"
     CDS             complement(2525..3592)
                     /gene="rlmN"
                     /locus_tag="U4A13_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1780795.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3822..6191)
                     /locus_tag="U4A13_002536"
     CDS             complement(3822..6191)
                     /locus_tag="U4A13_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1780796.1"
                     /translation="MLTIGVGFALILFAVFGFLKLGFLGILIANGFRIIAGNTYQILC
                     LLLAVLGFWIVIKNTEFSIGKNRRWFGGILFYFGILLLLHAHLFGKLHTGEPNIMGTT
                     WDLLASDIKQSQVDNNVGGGMIGAILYHFTYFLIAQPGSYLAAVLLLAGGAFLFSNLE
                     GYQLLNGIQSIGERVQELLEGDPAKQARKQAAKEERMKQRAEAKEARRLAAKEAAEKE
                     AAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQENIHPANLEKPVPDTPK
                     QTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIPTVDQSDEYKKIEK
                     NIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVNLTDDIALALAAKD
                     VRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVPLGRDVSGRVQTAD
                     LSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIPHL
                     LTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHPTL
                     PFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKAN
                     VPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVER
                     VVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQRR
                     FRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
BASE COUNT         1751 a         1346 c         1083 g         2075 t
ORIGIN      
        1 ggtacacggt gttccaaaag tagcttcttc ggaaataagt tggaatttca caaaaattca
       61 agaagtaatt ttcgtaaatt ccttcttatt tcttgaagct gattacttct gtcacaacct
      121 ctttttatta ttgagaattt ggtcgaggaa taatcgtttt tgccatccaa atatccggtt
      181 tgtcccagcc atttgcattt ggtaagacac ctacgatttt ataacctaat ttttcataga
      241 attcatacgg atgttcacga aggttctgga tggaagccac tttatcaaat gtatgctcat
      301 acaggtccgt ttgacttaac gttgttccat ggtctaaatc atccgtacct aaataaatcg
      361 tgattcctcc tctggaagct acttcttttt ctaagtaatt gactaatcga gtacctattt
      421 ggttctttcg tcgggagctt tctacaacta atggatgcaa ttcccaacct gtgataccgt
      481 attgagggat tgcaccaata aatcctacta actcatcttg gtctaccgct gctaccgcga
      541 ttcgttctgg attcatcatt tcttctactt cttctgccga gctgtctcca tattcttccg
      601 gccaagtcag tctcagtaaa tcagaaagct gatctttcaa tactggatta ttacggtcaa
      661 attcactgat tatcatacgt cttccccctt gttcttgctt attctaacag tccttttgaa
      721 aaaacgaaat gttctcgctc aaaaatcttt agctaccaat cagtatctct attctttcat
      781 tatttatctt acttataaaa taaaaaaatt atacagaaac actttattca aaataggaaa
      841 gatcaggaaa gccatcaatt ggaggaaaaa actctgtttg ttcatcatga tatagattat
      901 cttttgcttt aaaaaaaacc aattatgaat tgacaaataa aacctgtttt catataataa
      961 tcttaatttt tagaattttt agataattac aaaaaagcaa agggaatcgg tagaaatact
     1021 aagaggaaca aaaattcatt attgtcgttt tcaatgtttt tgtacttgtt cgtttttagg
     1081 atacttattg atgatttcaa ggtgttcatg ccaccttatt catcacttat cctgtcttag
     1141 gttcacctct actccatcta cagccgcagt cagatcgatc actctttaaa attttaaccc
     1201 gtttcaacga agaaagaagg aaatgaatat gagtcaaagt gttttcttac caaattatac
     1261 cattggaaca gatgcgtatg aaaaagcaaa tggaatcgtc aaagaatatg ggacaaaagt
     1321 agtgctgatt ggagggaaga ctgcactttc aaaggcagaa aacaaaataa aagtagcttt
     1381 gacacaggaa atagaggttg tcgccagtct ctggtacggg ggtaattcaa cttatggaaa
     1441 tattgaacag ttgatccagc atgaaagtgt ccaaaaagca gacgtcctgt ttgcagtcgg
     1501 tggtggccgt gcttgcgata ctgtaaaagt cattggtgaa aaactagcta aaccagtctt
     1561 cacctttccc actattggtt cgaactgttc gcccgtcact gcggtatgtg tcatctataa
     1621 cgaagaaggc gtaatggacg gactcttctt tccttcaaaa ccacctgtac acaccttcat
     1681 tgatacagca attattgccg aagcacctga taaatactta tgggcaggta ttggcgatgc
     1741 tttatccaaa gaagtcgaat ctactttttc tgctcgtggc gatgatctag gctacacgga
     1801 tagattagga atccaaatcg gaaaaatgtg cattgatcgt ctcttgactt atggtacgca
     1861 agcattgaaa gatagtgccg acgatcgtcc ctcatatgca attgaacaag tcgtttcgga
     1921 tatcattgtt actaccggat tggtttctgt tttagttaac aataactata attctgctat
     1981 ggcacactca ttctattacg gtacgactgt tctagaaaaa atggagggat accttcacgg
     2041 agcatctgtc agttatggtg tactgacctt attgatgatg gatcagcagt tcgatttgtt
     2101 tgataaaatc tatgtgttta tgaaaaagaa ccaacttcca gtatcgttaa ccgacatgaa
     2161 tctcaatgtc acgaccgatt tagaagcaat cttagataaa gccatggcaa caaatgatat
     2221 taaacatgtg ccttatgaaa tcacccgaga aaaatttaaa caggccattc ttgacgtaga
     2281 aacttatcat gctacacaag aaagccagca aggatataag aaaatcagtt aaataattgc
     2341 taaaaccccc atcaagcagg ctgtcatcaa attgaggaca tttctttgct tgatgagggt
     2401 ttttcttgtc aaaaaaagct ttatagcttt cgctatagaa tcttttctct tatttaagtt
     2461 gaacagttcc ttaattaaga aaactgctcc ttttatggta attgaaaaaa cgagtaggat
     2521 acgattattt ggcaactgtt tttttcattt gcttgcttcg caattgtccg caagctgcat
     2581 caatatctgt accatgttct ttacgtatca cacagtttac gccgtttttc ttcaatacat
     2641 cataaaatct caatacatca gcttttggac tgcgagaata ttgatcatgt tcactgactg
     2701 gattatatgg aatcaagttg acatatgcta attttttctt atcttttaat agatctgcta
     2761 attgttgcgc atgctctggt ctatcattta cttgattcaa catgatatat tcaaacgtga
     2821 ttcgacggtt tgttttctct aaatactcat ccacagcttc catcaatttc tcaattggga
     2881 aacttcggtt gatccgcatg atcgatgttc gcacctcatt attcggcgcg tgcaaggaaa
     2941 tagccagatt gacttgcaag ccgttattag cgaattcctt gattttgtgt gctaatccac
     3001 tagtggaaac tgtaatgtgc cgagcaccaa ttgccaatcc tttggcatca ttgatgatat
     3061 gcaaaaagtc cattacattg tcgtaattat caaatggctc accgatcccc attacaacga
     3121 catgtgaaac gcgttcgcca agattgcgct catcaaaata atgttggacc atcatgatct
     3181 gtgcaacaat ttctccagca gtcagatcac ggttcttttt gagcagcccg cttgcacaaa
     3241 acgtacagcc gatattacat cctacttgtg tcgtcacaca aactgacaag ccatattctt
     3301 gtctcatcag tacagtctca atcatgtttt tatcagggag ttcaaacaaa tattttactg
     3361 taccatccga agcttcttgg acgaccatct gttttagagg attgatgata aaatgctccg
     3421 ataatttttc gatcaattgt ttcgatagat tcgtcatttc tgtgaaatta cttacacgtt
     3481 tttgatacaa ccattcccaa acttgggcag ctcggaattt tttctcgccg ttttctataa
     3541 accaattgat taattcttca ttcgtcaagc cataaatgga ctgtttttcc aaagtatttt
     3601 ccctcttttt ctcaaagttt cgctacatac tatacatgat tcatttcctt aatgcaatac
     3661 ttcacaaata aatcgaatag aactttaatc tcacaaaaac taaaaagaca agacatcata
     3721 ccgtattaga aaagatggaa aaaaaccatt ttttcttttg gtatcatcat gccttgtctt
     3781 tttattggtt attcgttttt caacccttca tcacacaagt tttacatgtc attatcgtcc
     3841 ggcaacgttt ctgatgtttc ttcctgctca atgaatactt tccgaggctt gcttccttct
     3901 gacgggccaa caactccatg agcttccagt tcatctacta aacgtgctgc tcggttgtaa
     3961 ccgatccgaa atcttctttg gagaagggaa atacttgccg tttgcatttc aacgacaagt
     4021 gcttttgctt cttcaaacag ctcatcttgt ggctgttctg gagcacctgt cgtttcaact
     4081 tcatctgtcg gcatcatttt ctcttcataa tgagcttctt gctgatctgt aacaaattga
     4141 acgacccgtt ctacttcgtg gtcagaaatg aatgctcctt gcacacggat cggtttattc
     4201 tcacccattg gcaagaatag catatctcct cgccctaaga gcttttctgc cccattagaa
     4261 tcaatgatcg tccgagaatc tgtaccgctt gataccgcaa atgccattct ggaaggtaca
     4321 tttgctttga tgattcctgt gataacatcc acacttggac gttgagtagc taggatcata
     4381 tgaatccccg ctgcccgagc catttgcgct aaacgaataa tcgcatcttc aacttcattg
     4441 ctggctacca tcatcaaatc agctaattca tctacgatca ctacgatgaa tggtaaggtt
     4501 ggatgtttcg ttccgttttc caagtttttc tgttgaacaa attcattgta accagaaata
     4561 tttcttactc cggttgccgc gaatttttca tatcgttctt ccatttcttg tactactttt
     4621 tgtaaggctt gcgctgcttt tctagggttc gtcacgaccg gcgtcaacag atgaggtatg
     4681 ccgttgtaca tattcaattc aaccattttc ggatcgatca tcatcagttt tacttcatgg
     4741 ggcttagcac gcattaatat acttgtgata atcccattaa ttgctactga cttcccacta
     4801 ccagtcgaac cagccacgag taagtgaggc atcttcgaaa gatcagccgt ttgtacccgt
     4861 ccagaaacat cacgtcctaa aggtacttct agtaatttat ctgggtggtt tggttgtgct
     4921 tcgatgattt ctctaaagga aaccatactg atctttccat ttggtacttc aataccaatc
     4981 agtgatttac ctggaatcgg tgcttccatc cggacatctt tcgccgctaa agcaagcgcg
     5041 atatcatctg ttaagttcac aattttactt acttttaccc ctacagccgg ctggacttcg
     5101 aattttgtga cggatggtcc taagctggct ttgacgacct tggcatcgac accgaaactt
     5161 tggaaagttt gttctaatac gccgatattt ttttcaattt ttttatactc atcactttga
     5221 tccacagttg gtatagaatc aagcaaatca actgtcggta attcatagtc ttgattttcc
     5281 tgttctgctt caatctcaaa ttctaatgag gtaccgtcat cctctggcaa ctcatctgaa
     5341 aaaccttctg ctgtattggt ctgttttggt gtatctggta ctggtttctc tagattcgca
     5401 ggatggatat tttcttgaaa actgtcgatt ggaacaaaac ttagctgctc cggctctgta
     5461 ttttctggtt cctgccattc atcagcagta ttttcccttt gctggctcac cgcttttttc
     5521 ttctcatatt ctgcagcttc tttttctgca gcttcttttg cagctaagcg ccttgcttct
     5581 ttcgcttctg cgcgctgttt cattctctct tcctttgccg cttgctttcg agcttgcttg
     5641 gcaggatcac cttccaataa ttcctgtact cgttctccaa ttgattggat accatttaac
     5701 agctgatagc cttccagatt actgaacagg aatgctcctc ctgccagtaa tagcactgct
     5761 gctaaataac ttccaggctg tgcaatcaag aagtaagtaa aatgataaag gattgctcca
     5821 atcatcccac caccaacgtt attgtctact tgactttgtt ttatatcact ggctaacaaa
     5881 tcccatgtcg tccccatgat atttggttcc ccagtatgga gtttgccaaa taaatgggca
     5941 tgtaacaata ataagatacc gaaataaaag agtatgccgc caaaccagcg tcggttttta
     6001 ccaattgaaa actctgtatt cttaatgaca atccaaaatc ctaatacggc taataaaaga
     6061 cagaggattt ggtaagtatt tcctgcaatg atccgaaagc cgtttgcaat caatattcct
     6121 aaaaaaccta attttaaaaa gccgaagaca gcaaataaaa tcaaggcaaa cccaacacca
     6181 atcgtcagca tttgttcctg ctgctgtttt tgctttttcg tttttttcgt ctttttcctt
     6241 tttttagtag gccgt
//
LOCUS       DASJPC010000123         6173 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJPC010000123 DASJPC010000000
VERSION     DASJPC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.125"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="U4A13_002537"
     CDS             725..811
                     /locus_tag="U4A13_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1780797.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U4A13_002538"
     CDS             complement(932..1621)
                     /locus_tag="U4A13_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780798.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="U4A13_002539"
     CDS             complement(1628..1906)
                     /locus_tag="U4A13_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780799.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="U4A13_002540"
     CDS             complement(1866..2780)
                     /locus_tag="U4A13_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN1780800.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U4A13_002541"
     CDS             complement(2762..3142)
                     /locus_tag="U4A13_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN1780801.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="U4A13_002542"
     CDS             complement(3592..3879)
                     /locus_tag="U4A13_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1780802.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="U4A13_002543"
     CDS             complement(3899..4123)
                     /locus_tag="U4A13_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN1780803.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4793)
                     /locus_tag="U4A13_002544"
     CDS             complement(4578..4793)
                     /locus_tag="U4A13_002544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780804.1"
                     /translation="MEKMVAAMEMKKEILMTKKHLLFGSILLGILSFSPMLLSVANKG
                     NKKGELKLNPNYYNSQNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="U4A13_002545"
     CDS             complement(4763..5296)
                     /locus_tag="U4A13_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1780805.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="U4A13_002546"
     CDS             complement(5317..6159)
                     /locus_tag="U4A13_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1780806.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DASJPC010000124         6146 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJPC010000124 DASJPC010000000
VERSION     DASJPC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.126"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..2542
                     /locus_tag="U4A13_002547"
     CDS             101..2542
                     /locus_tag="U4A13_002547"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN1780807.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="U4A13_002548"
     CDS             complement(2872..3576)
                     /locus_tag="U4A13_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780808.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="U4A13_002549"
     CDS             complement(3763..4008)
                     /locus_tag="U4A13_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780809.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="U4A13_002550"
     CDS             complement(4025..4876)
                     /locus_tag="U4A13_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN1780810.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="U4A13_002551"
     CDS             5042..6046
                     /locus_tag="U4A13_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN1780811.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1970 a         1064 c         1301 g         1811 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtg
//
LOCUS       DASJPC010000125         6108 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJPC010000125 DASJPC010000000
VERSION     DASJPC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.127"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(182..1144)
                     /locus_tag="U4A13_002552"
     CDS             complement(182..1144)
                     /locus_tag="U4A13_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1780812.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="U4A13_002553"
     CDS             complement(1146..2585)
                     /locus_tag="U4A13_002553"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN1780813.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="U4A13_002554"
     CDS             2770..4716
                     /locus_tag="U4A13_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN1780814.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /locus_tag="U4A13_002555"
     CDS             4980..5852
                     /locus_tag="U4A13_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN1780815.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1945 a         1049 c         1077 g         2037 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaat
//
LOCUS       DASJPC010000126         6001 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJPC010000126 DASJPC010000000
VERSION     DASJPC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.128"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..395
                     /locus_tag="U4A13_002556"
     CDS             <1..395
                     /locus_tag="U4A13_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780816.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="U4A13_002557"
     CDS             398..1228
                     /locus_tag="U4A13_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEN1780817.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="U4A13_002558"
     CDS             1244..2095
                     /locus_tag="U4A13_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN1780818.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="U4A13_002559"
     CDS             2092..2451
                     /locus_tag="U4A13_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEN1780819.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="U4A13_002560"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="U4A13_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="U4A13_002561"
     CDS             2624..2929
                     /locus_tag="U4A13_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780820.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="U4A13_002562"
     CDS             2929..3240
                     /locus_tag="U4A13_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN1780821.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="U4A13_002563"
     CDS             3237..3410
                     /locus_tag="U4A13_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780822.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="U4A13_002564"
     CDS             3407..3823
                     /locus_tag="U4A13_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEN1780823.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="U4A13_002565"
     CDS             3823..4491
                     /locus_tag="U4A13_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEN1780824.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="U4A13_002566"
     CDS             4488..4865
                     /locus_tag="U4A13_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780825.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="U4A13_002567"
     CDS             4862..5161
                     /locus_tag="U4A13_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780826.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="U4A13_002568"
     CDS             5151..5309
                     /locus_tag="U4A13_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780827.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="U4A13_002569"
     CDS             5403..5819
                     /locus_tag="U4A13_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN1780828.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DASJPC010000127         5924 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.129, whole genome shotgun sequence.
ACCESSION   DASJPC010000127 DASJPC010000000
VERSION     DASJPC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.129"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(75..1169)
                     /locus_tag="U4A13_002570"
     CDS             complement(75..1169)
                     /locus_tag="U4A13_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1780829.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="U4A13_002571"
     CDS             1358..2119
                     /locus_tag="U4A13_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN1780830.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="U4A13_002572"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="U4A13_002572"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN1780831.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="U4A13_002573"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="U4A13_002573"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN1780832.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4772)
                     /locus_tag="U4A13_002574"
     CDS             complement(3759..4772)
                     /locus_tag="U4A13_002574"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN1780833.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4937..5857
                     /locus_tag="U4A13_002575"
     CDS             4937..5857
                     /locus_tag="U4A13_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN1780834.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1180 c         1063 g         1875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DASJPC010000128         5809 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJPC010000128 DASJPC010000000
VERSION     DASJPC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.130"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            450..1604
                     /locus_tag="U4A13_002576"
     CDS             450..1604
                     /locus_tag="U4A13_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN1780835.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="U4A13_002577"
     CDS             1598..2020
                     /locus_tag="U4A13_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780836.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="U4A13_002578"
     CDS             3143..4075
                     /locus_tag="U4A13_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1780837.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="U4A13_002579"
     CDS             4092..4607
                     /locus_tag="U4A13_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN1780838.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="U4A13_002580"
     CDS             4903..5184
                     /locus_tag="U4A13_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780839.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="U4A13_002581"
     CDS             5186..5602
                     /locus_tag="U4A13_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780840.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DASJPC010000129         5790 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJPC010000129 DASJPC010000000
VERSION     DASJPC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.131"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U4A13_002582"
     CDS             complement(99..1229)
                     /locus_tag="U4A13_002582"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEN1780841.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="U4A13_002583"
     CDS             complement(1877..3079)
                     /locus_tag="U4A13_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN1780842.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="U4A13_002584"
     CDS             complement(3139..3342)
                     /locus_tag="U4A13_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN1780843.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="U4A13_002585"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="U4A13_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="U4A13_002586"
     CDS             4879..5691
                     /locus_tag="U4A13_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780844.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DASJPC010000130         5749 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJPC010000130 DASJPC010000000
VERSION     DASJPC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.132"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1128)
                     /locus_tag="U4A13_002587"
     CDS             complement(<1..1128)
                     /locus_tag="U4A13_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1780845.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
     gene            complement(1164..1874)
                     /locus_tag="U4A13_002588"
     CDS             complement(1164..1874)
                     /locus_tag="U4A13_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN1780846.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            complement(1942..2757)
                     /locus_tag="U4A13_002589"
     CDS             complement(1942..2757)
                     /locus_tag="U4A13_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEN1780847.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            complement(2768..3736)
                     /locus_tag="U4A13_002590"
     CDS             complement(2768..3736)
                     /locus_tag="U4A13_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1780848.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            4499..>5749
                     /locus_tag="U4A13_002591"
     CDS             4499..>5749
                     /locus_tag="U4A13_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1780849.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT         1772 a         1183 c          915 g         1879 t
ORIGIN      
        1 attttttata acagttgaca tgtaagcctc ttctaacgtt tgattcaagc cgagaatttg
       61 tggatatacc tctgctacta cttgtccttc gttcattttt tctgctgtct tttgagtaac
      121 catttccaaa tcactaatcg aattaaaaac gccttcaggt aaccgctttc caaattcgac
      181 aagaattgac gcagctttat aacccgcttt atctccttcc gttgtcaaac agcaaggaat
      241 ctctacttca ttgaatgcag caacttctgc tttaattgcc tcatagtcaa tctcgggtga
      301 agctactcct gactgataaa caccgtaaat atctccactt ctatccgcaa caacaccatc
      361 taccatgatc ttcactccac caaagcggac cttttcaggg aaaacttttt ccttatactc
      421 atatagaagt tcatgctgta gaggttcttg gactgcctgc acatataggt tactaagtat
      481 tttcctctga ggtatattag aaagaaattc tgcgtcatca aaaacgattt ctttcattga
      541 tataacacct tttgaaagca gtaatgcttc aaattcagcg taaacctctg ctaaaagctt
      601 tttgtttgct agcatttcct taattaataa tactcgactt tcagcactgg tttctttttc
      661 tgtaaaacca taagtctgct tagcaatagc attcatccaa caatggctgc gatttttgtc
      721 gatcgctgtg attggaccaa agtggtttat ttcattcaat agctcttctg atgggtcagt
      781 tttccacagg gcagcatccc agccatgagc ataaattggt tcatctgctt ttttatggtt
      841 cattacagct ttaatacgct ctacagcctc gttagctgaa gatgtatcgc ttaaatctat
      901 gccagcattc atagccaaat aaccactgaa aaatacatga ttatcatgaa ttgctggcat
      961 aacgaattga tcatcataag caattaattc cgtgttttca tttaatagat cactgtaatt
     1021 ccagttgttt tccacctttt caatgatccc ttcattgatt acaatataac ctttgaaagg
     1081 tggctgctct ccatcaaaaa tcatttttga ccgcaatatt tgcttcataa ataaaatcta
     1141 ctccttgaaa tccaaacagg aaatcataag taaaaacgat atacttctcc caggtaatat
     1201 tgattaccta catgaattgg tatgttgttt tgatcgttga ttaaagtatc cataaagaaa
     1261 aagggcgtcc caacagctac ttccattatt ttggcaattt ggtcatcagc tgtgacaatt
     1321 tcaagtgcag tttccccagg attgatggct aaggtacctt ggactttaag tacttcatac
     1381 aaagaatcag tcagctcttc ctccattaag aaactatatc tttcataaga aaaataattg
     1441 ttttctaaca taatggggat gttatccgcc attcgctttc tctggatata taaaacatca
     1501 tcaccttgag cgatatcgag tttttttgcc atcccttcag ttgctggaat gatcttgcgt
     1561 tccaaaacaa tgctagtcgg tgtaaaacca ttagatttac aactctcact gaaacccata
     1621 atatattcaa ttttacgaaa aactttatgc cggttgacaa aagttccttt cccttgtttt
     1681 ttaagtaggt acccttcttc taccagttct tctactgctt ttctcactgt gattcggctg
     1741 acctcataac tttctatcag ctctgattca gcgggaattt tcgtattctc gggatactcc
     1801 cctgtattga ttttattttt taatatttct tttaattgta agtacaatgg taattgtgca
     1861 tttgtatcca gcattttttt aacctccact ttaccgggat ttctttcttt aggttatcaa
     1921 atgcaggaat ttaaaacaag ctcaatcgta atgaatacca aagccgaacg aaccgtcaac
     1981 cagacaagac ttcccagcaa attcattccc ctttttcatc gcggtgacta tatcgtcccc
     2041 ttcgagaaaa taaagtagga aagctgttaa gaaagaatct cccgctgcca ttgtatcaac
     2101 tggttcttct agcacggggt tcacttcata aaactttgct ccatcataac caatagccgt
     2161 tttttctcct cgggttgcaa tcaatacatc atttcgctca caaaaatgct tcatcaaaaa
     2221 ctgctttact tcttcatcgg aaaagtcttc tatagaaaag aagccaaaat caactttatc
     2281 aattgcccgc tgaacctctt cttccgtaaa gtcgtcagaa aaatcataag aaatctttcc
     2341 agaaacattt tgtaactctt ctaatagatg attgacatgg ctatataatc ccatatgaat
     2401 caattcaaac tggtttaaat attctctgtc ttcttcagta agctgaatcg ggtattcacg
     2461 agtaacacct cccgaattgc tgccaaggaa aatccggtcg ccctcatcaa tcttcactct
     2521 ggcacagcca ttctcgcctt tttcaaagcg agagtgactg ttgtccagtc ctatttcttt
     2581 tattgctgat aaaacatgag ctgcctcctc atctgagcca aaaactccca taaagctact
     2641 ttcatgtccc agtttttttg caaaaactgc aaaattcaat gaatttcctc caggatacat
     2701 aattcccaga ttttcatatt tatcgacgac gttatcacct aagcctaata ccttcatttt
     2761 aatcctccta gtactccact ttccccatat agcgacgaac atctaaatca tgattgcgta
     2821 catctgctaa agctgaacga tattctgaaa gaatactgta gaataaaatg ggattgaaga
     2881 attcagaaac ttcatctgat atcatatcca atcctagttc ctttgcatca acgatttcta
     2941 ctctttctgc atatttatca aggaatcgtt ttgcccgttc atctaaagca cgagtgcgtc
     3001 caatgctcat caagagaata aaattcgtat ctttgtctgt tacttcaaaa ggtccatgga
     3061 agaattctcc agaatgaaca gaagttgaat caagccattg catttccatc aaagagcaaa
     3121 ttgaaaaacc ataagcatgt ccatatgatg gtccactacc taagatataa aacattgttt
     3181 catctttgta cttatcagca aacactttaa tacgatcctg cacctgctta atgccatttt
     3241 caattacggt atcgataacc ttcatgcctt ctctgaattt accgtaatgt tcaaatgttt
     3301 cggtaacatt gaccaaagat acagttaaat caagaatgat ggccatagga ttattgacta
     3361 tttttgcatc atctccccat tcgtaaagga aactatgatc agaaaccttt aataaatccg
     3421 cagttttatt atgagtcaga ccaacagtca cagctcctct agcttgagcg acttctcctg
     3481 cttttaccgt ttctgctgta tttcctccat gagaacatag aatgacaaca gatttatcat
     3541 ctaattgctt tggggttaca tggttaaatt cgttcgctgt atataagcct gtaatacact
     3601 tcgatgcttc ggctttaaga aaataatctg agacatacat atctaccagc gaaccgccac
     3661 aagccaccaa ataaattcca gaaatctctc catcattttt ttctacaatt gcgttaaccg
     3721 tttttgctac atccatcaac atttcctcct taaaatttta aaaatgttaa ttttatccaa
     3781 aataaaagta actgattaaa tgtaaagata tcacataacg tcatataacg tcatgtgaca
     3841 taattgcatt gtatccgctt tctttgtgtt tgtcaatagg aaaaatttat ttctctaaaa
     3901 aatataaata atctgcagtg aaacttgagt acatatacta atttcgaaaa atgcctatga
     3961 tttaaataat tatggtgctg ggatgaaggt ttataagttt gtcttgattt gtttagaaga
     4021 aatcgttttt gcacacagtt gaattaggaa actactggag tggaaaataa agtgatttga
     4081 cgggtagatg ggtgcactta aatttgaaaa tggcttaaaa tgctttattt tgaattgcag
     4141 aatttctttt agtacgcaat ttatggtctt aaaactgatt aaaaagtgat acattgattt
     4201 taatacaatt tatgatacaa aaaatgacct cctttcttaa ataaaagagg tcattttgtt
     4261 ttgtctcata atcgattgtt tttgatacag tagttatatt cagaaaaaat aaagactctt
     4321 ctgagtttat aatcctttgg attatatacg gatacagatg tagacataaa acaagctcaa
     4381 ttttcaaaaa tggcattact cacctttttg gtgaattatt tgtataaaaa aaatatagaa
     4441 aagacaccct gaaatccttt agaattaatt tggctaccaa ttcataggag gattcaagat
     4501 gtctcatact cagcttatca aagataccct aaatattcta gacctaaata tctatttcga
     4561 ggaaaattgt ttaactaaag aaaaatacaa agggcaaatt tgcttgattt acaaaggaac
     4621 tcttctgtac aagccagaag agtgttccca ttgtctttgt gtcgtcccct ctaggatcat
     4681 tcgttggggg acgacaaccg ttcggttgtt actgaatgat gtgtctgagt atcgcactta
     4741 tctggagctg aagaaacaac gatttaaatg taagtcttgc caaagaacat tcgtagcgga
     4801 tacctctgtt gctgaaaaac actgtttcat tagtcaaaaa gttcgttggt ctgtcattgc
     4861 tcgcttaaaa gagaatactt ccatgacgga aatcgctcgt cagaaaaata tttccacttc
     4921 ttcggtctat cgtgtcatga agcgattcta ccgcccattg aatcctttca aacagacttt
     4981 gccaaaggtc ttatgctttg atgaattcaa atcggttcga agtgttgcca gtgcaatgag
     5041 ttttatcatg atggacggac aaagccatgc attattggat attgtcgaaa atcgacgctt
     5101 gccttattta gaacgctatt tttctcggtt ttcattagcg actcgtgaag ccgttcaatt
     5161 gattgttatt gatatgtatg cgccttatgt ttcgttggtg aaaaagcttt tcccgaatgc
     5221 tcagttgatc atcgatcgtt tccacatcgt ccaacatatt ggcagaactt tcttgaatca
     5281 tcgagtgaaa gaaaccacgg tacgacttaa aggaaattcc cactctcaac gagggttagg
     5341 gaaaaaattg aagcgctatt ggaaactcct acaaaaagaa gaagcaaagt taaactatga
     5401 aaaacgggtt tggcgtgcca gttttaaaga ttatttgact gaaagtgaga ttgccgatcg
     5461 actgttagct gcttgtccca acttacgcca aggctatcag ttataccaag acattcttta
     5521 tgcggtaaag aaaagagatc agtcactatt tgaggattgt ttaactagag acattagcgg
     5581 tctgccagaa acctatacaa ccactttacg aacgtttaaa aagtttctgc cacagatcca
     5641 aaatgcgctt cactacagct attcaaacgg accattggaa tgcttaaaca accacatcaa
     5701 agtattaaaa cgcaatgcgt acggctttcg caacttttac aatttcaaa
//
LOCUS       DASJPC010000131         5714 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJPC010000131 DASJPC010000000
VERSION     DASJPC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.133"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..675
                     /locus_tag="U4A13_002592"
     CDS             10..675
                     /locus_tag="U4A13_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1780850.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(959..2044)
                     /gene="xerS"
                     /locus_tag="U4A13_002593"
     CDS             complement(959..2044)
                     /gene="xerS"
                     /locus_tag="U4A13_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN1780851.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2378..2611
                     /locus_tag="U4A13_002594"
     CDS             2378..2611
                     /locus_tag="U4A13_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN1780852.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2608..3015
                     /locus_tag="U4A13_002595"
     CDS             2608..3015
                     /locus_tag="U4A13_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN1780853.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4152..4670)
                     /locus_tag="U4A13_002596"
     CDS             complement(4152..4670)
                     /locus_tag="U4A13_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN1780854.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            5037..5285
                     /locus_tag="U4A13_002597"
     CDS             5037..5285
                     /locus_tag="U4A13_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780855.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1912 a          919 c          908 g         1975 t
ORIGIN      
        1 ttctttaaaa tggagttaag agaaatacgt ccaattgaca ttcaaaagtg gcaaaataat
       61 ttattgagca aatataaaaa ttcttatgta agaaacatgt atgggatttt tcagatgtca
      121 ttagatcgag cagttgtttt gggactactt gaaaaaaatc ctgcaaaagt tattggaaat
      181 gtgaagaaaa ggaaaactac agttgatttt tggacaaaag aagaatttac aaaagttatt
      241 tcaacttttt atattgatga tttgtaccaa cattttggtt ttatttgtat gtggttactt
      301 tttatgaccg ttatgcgttt gggagaagcc actgcattaa cttggaaaga tattgatttt
      361 gaaaataaaa acttgcgtgt gaataaaaca ctctattata agagagccaa aacctatgag
      421 tttgtagaac ccgaaacggc ttctagtatt cgtacaatcg aactagacga caatacaatt
      481 acctttttaa aagcttggaa ggagcgtcaa gtttcaatag gaggcatgtc gtttgtactt
      541 tcttataatg gaacacctac tcagaaatat acaatccgtc atattatgag aagacatgca
      601 acgatcgttg gtgttcatat aatccgcata cacggattaa ggcatagtca tgcttctttg
      661 cttatatcac aataaaaaga tgcacttcga attaaagaaa ggttaaaaca tgctgatgta
      721 caaacgactt taggaactta tggtcatttg tatctaaatt cattgagaga gataacaaat
      781 gatcccacag attatatcca aactaatttt tctaaaatca aaccgacacc tatttacgat
      841 aataaaaaat aaatgataaa caaaagtagg atcacaccaa aagaaacaca ctagaaaaac
      901 tataatacag gcttttctag cgtgttgttt cttatttccg cctatttata cattatactc
      961 ataaagcatt taatgcatcg atcgtagccg cattgtctat atgggtatat aaagttgttg
     1021 tattggtact tgaatgtccc aattgttggg cagttaatac caatgaattt gtctgttggt
     1081 agagtctagt agctaatgta tgacgcagtt tgtgagggct gacttgaatt ctaaagcctt
     1141 ttgaatattt tgctaccatc cgttcgattg caataccgga tatacgcttg gcttctcctt
     1201 ggtaaattgc tagaaataaa gctttttcgt tttctgttac tttatatcgt tctttccgaa
     1261 tagctagata attagctaaa tattccattg cgaatgctgc tatattgaca gcatctcttt
     1321 tatttccttt tctagttact accacattat tcttatctaa agataggtca tctaaattaa
     1381 tatttataac ctcggagagt ctcaatccag atcctaaaat cagtgcattg atcgccaggt
     1441 ctctttctac atctcgttga taatagatca aagctcgttt actcaaagta tttttatatt
     1501 cattggcaat ataatctaga taattttgtg tatccttatc tagaaataat tttttctcaa
     1561 tggcattggc acgtgcagct aacgtagttt tatctgtgat ggttgctact tgatccatag
     1621 gattattgtc taagtatggt tttcctagag gattattgga acttttcgaa agatatttaa
     1681 atagaacctt taatgccgtg atatttctat tgatagtaga agtagataga cctaagttat
     1741 tattatcttt gagcttagtt tttgttgtct ctttttttac attttctttt gtgcgacgtt
     1801 tcaaatgaga tttgtacaag tttaggtcat aggcagttaa ctgtcttatc gaggtgatag
     1861 aagtatcagg aacgatagac tcactcagaa gccattcata aaagagctta taatttttta
     1921 gatattctaa agtggttcga ggtgattgat ctttatcctc acgatcttga acaaaatctt
     1981 ttatataatc gggcatatga agtatgattg tatctatttt ttgatatatt tctgtatttt
     2041 tcatgtcatt catcctttca ataagtaggg gagaagagta tctctataat tggactatac
     2101 caattataga atgttttgtc aattggtatg ctctaaaatt tattaatttg tttcaaaaaa
     2161 agcatataaa caggtatacg tttaataatt taaaataaat ggtatacata aatagggaag
     2221 gaaatagaag ggagcaatct aaattttttg aattttctta attaattata tagaaagaac
     2281 aatctttcta tagagttaga catgagaggg actatgcttg atttaaaatc aacaagcgta
     2341 tatactgtat atacaaagaa aaaggaggga gaagactatg gcaattaaag aaagaaaatt
     2401 tagggaagtg gggaactcag tagtattaac tatttcaaag gagtttctta aagaaaatgg
     2461 gttaaatccc ggagatacgg tttttttaga tgaagaaaaa cttcgagcgg caattacaaa
     2521 aaaacaagcc ttatctgatg aaattgattt agcaatatct caagcattaa ctgaatatga
     2581 tactactttt agagagcttg tcgataaatg agtattcatt acctagaagc caaagatatt
     2641 ataaaaatga atgtaatcca aataaaaaaa tactctccta atgaacccct tggtgtaaag
     2701 gatgcgaatg ctttagaaat gtgtgtaggg caacttcgag cctcagcttt tggtgaagag
     2761 gtttatcctt cgatttatga aaaagcagct atcctactta tacaattgat aaagaaacat
     2821 ccttttcaca atgcaaataa acgtactgct ttcttagcta cttttgtctt tttgaaaatc
     2881 aatggacagc ttttagctat tgatcaaaaa gatgctgtaa atttagcggt atatattgct
     2941 acgtatgata aggatttcga tcaactaaaa tatgaagtga tcaacactat aaagacacat
     3001 acgcagccca tttaaggagt tactgtatgt gtctttatag ggttattagc tgctatagcg
     3061 aaataattga agtaaattac aaatagctat aattcaaatc caactatata gaaaacgtaa
     3121 attttctata tagttggatt aagataacga ctatttattt tatcatattt ttactatacc
     3181 ctatactttt acttggtata tttatgaata atcgcaaatt tacccctata ctactagttt
     3241 gtaagttaga ttaataaaaa tactaaagtt atttgttctt ttattaaata cacctacaac
     3301 ttataaattg atggtattta gataacacga aagaataaaa ataacagctg atagtaaagt
     3361 ttgttgacga ttgctactca atcgaaagtg agttagtgcg tatgaacttg cttctgaaaa
     3421 cttatgaaat gaaagacctt ttacatcttg cagaaatcgg tactgggatt aaactaagca
     3481 ataaaaacta atgttcccct cattctttga acttctacta tttgatttat ttattctgat
     3541 aaatgacgat ttcaccttca ggatcttcaa atcccgtata gaatttattt aagatctctt
     3601 cagtatcagt aataggatag gcgataggcc acataccctt tgatggttag aataaactgc
     3661 tcaaaatctg tatcgtatgt tggctttagc atattaacaa aggctatctc caaattataa
     3721 accataagcg ttgaatcgtt cttaacaatt tcaattcttc ctaacccaaa gtcagaagat
     3781 ataattaccg gacttgcctt acccttcatg tgggttgttg agattccata tttaaaggga
     3841 attgtcggta ctatcggcgg tctgaaaaac atcccaaaaa taaaaaaatc tttcccctgc
     3901 tagtctccta agtccctttg tagcggatat ggcaccataa tctcatttta tcgaagtgag
     3961 tcatggttta tctgttggcc aaccaaaatt gatacaatct aacgatgcac cctctacggc
     4021 attctagggg agtgcacttg cattcaccca ataaaaaccg gcagttagca aagaacttat
     4081 gtagtcgtat gttcttactg ttaacataca agaggtctcc tttccacaat tgcttagcag
     4141 taaacgggaa tttacaaagc taatctcttc cattttctta tctttgttct aaaagtccca
     4201 aatggagcaa ctgtatttac atgaataaat ttatatactt cccatacagc cgttttggtt
     4261 gcctcgtctg cccattttct tttgtgtgca gtgaatagat cttcatcact cattgaatca
     4321 atcatcatgt taatatcatc aatattacga ttgagaattt cctctaattc tttcaatggt
     4381 agatgtgcat aggtatttgt aaaccactta tataactctc ctaactgatt ccatttaaat
     4441 tgatcagaag gcgttttaac agataatcca tttctttcat cattttccca ttttagaatt
     4501 aaattcgtcc aaccaacttg atatgctaaa ttttcagcag gtgttctatc cacttcctca
     4561 actcttttat ccttcaattc ttcaggaata gtttcaaatt ctaaaatata tttagtataa
     4621 gtcctattta tttctttttt taattcttct ttatttttat atgttctcat tatattacct
     4681 ccataaattc ctaattgtct cctttaattc atctataatt tcatccacga tattttaatc
     4741 cgttctacaa tgcatatctg atatgcattg actgtctaac tcatatgtga atgatttata
     4801 aaatatccca acgtctcatc taaagaaatg ttgatcgtca gcaaggagct aaaaaatacg
     4861 aatatatttc ttttgtagta aaatcggact ggtttgcata tcattttctt cgtaaatatc
     4921 aaaatcatgt taactactat caattactaa taaaaactac cactttaagc gttaagaact
     4981 gttaagttta gttaagtgag gatagttcac aaaatccatt gttaaacttc acgaaagtgg
     5041 taaaactcaa aatgctttat ataggattac agcatcgctc tttcttcgat tgcgcgttgg
     5101 gtaaaaaata ttatgaagta aaaattgacg atgacacgtt ccttacggct catcagatga
     5161 aacagctaca aaaacgaaat gatcaactcg aagaaaattt aattctaaaa aagcgattgc
     5221 catattcacg cctcgctcga cgaactatta aaggtcaaga tttacctgaa gatcatcctt
     5281 tttaggaaat tgaagctttt tatgttgcac aatgcattta taatactaca atgatgtttt
     5341 caccagatat tcttctaggg tgcggtgtaa tgaaacagat acacttgcaa aagaaaattc
     5401 accgcgaatt tactaaaatt atgaataatt atgttaatct tccgaaattt ttttccaaaa
     5461 ttaagggatc aagctggtgt tatagattgt ttaaggttgg caaaaaaata acttcttttt
     5521 aaacaattat agaaaaccga tactctgtta agttggttga cacggtgtcg atttttatga
     5581 ctttcttatt ctgaaacttc actaacggac agtacctaaa aagtctatag tatggacgtt
     5641 tacgagtact attttatcaa aaatgctgct catttcaact tatatgtttt agtgaactac
     5701 ccccacttag cttg
//
LOCUS       DASJPC010000132         5614 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJPC010000132 DASJPC010000000
VERSION     DASJPC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.134"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(59..2008)
                     /locus_tag="U4A13_002598"
     CDS             complement(59..2008)
                     /locus_tag="U4A13_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN1780856.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="U4A13_002599"
     CDS             complement(2050..3108)
                     /locus_tag="U4A13_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEN1780857.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="U4A13_002600"
     CDS             complement(3227..3682)
                     /locus_tag="U4A13_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780858.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="U4A13_002601"
     CDS             complement(4266..5069)
                     /locus_tag="U4A13_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1780859.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2076 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat attt
//
LOCUS       DASJPC010000133         5569 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJPC010000133 DASJPC010000000
VERSION     DASJPC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.136"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(17..238)
                     /locus_tag="U4A13_002602"
     CDS             complement(17..238)
                     /locus_tag="U4A13_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780860.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(235..525)
                     /locus_tag="U4A13_002603"
     CDS             complement(235..525)
                     /locus_tag="U4A13_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780861.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(522..1706)
                     /locus_tag="U4A13_002604"
     CDS             complement(522..1706)
                     /locus_tag="U4A13_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1780862.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(1888..3291)
                     /locus_tag="U4A13_002605"
     CDS             complement(1888..3291)
                     /locus_tag="U4A13_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN1780863.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3313..4086)
                     /locus_tag="U4A13_002606"
     CDS             complement(3313..4086)
                     /locus_tag="U4A13_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780864.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4096..4473)
                     /locus_tag="U4A13_002607"
     CDS             complement(4096..4473)
                     /locus_tag="U4A13_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1780865.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4494..4808)
                     /locus_tag="U4A13_002608"
     CDS             complement(4494..4808)
                     /locus_tag="U4A13_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN1780866.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5008..>5569)
                     /locus_tag="U4A13_002609"
     CDS             complement(5008..>5569)
                     /locus_tag="U4A13_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1780867.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1650 a         1097 c          940 g         1882 t
ORIGIN      
        1 tcatgaaaaa tatctgttaa gcaccaatga ttcggttaaa taagtttaat aacacatctt
       61 tcacgccagc tgcattgaac acaagtccga cggcgattag agcaataatc aagaagccaa
      121 tgagttttga aaattctcgt ttaaaaccta aaaacagtcc aatgacaaca atcgctaaca
      181 ataccaatga ctgtgcatta tccaagaacc agttgtacaa attctgccca aaattcatgt
      241 gattgtcctc ctttccttaa gatatttttc tcgataatag atatagcttt ttacacgcct
      301 taataattga aaaggggtta aatgctctcc atagacggtt actcgattgg tataattatt
      361 ataaaatctc accttgtaaa aatcataggt atagatggta tcaccagctt gccgttttcc
      421 ctcaataatg agagacgtat atttccctgt attttgtata tacgcttgaa actcaatgcc
      481 acactgaaac cctaacttct ttaattcttt tgttaaaagg ctcatgtaat caaatcctcc
      541 atggctagat gttgttgttc aattaatttc tcatgttttt ctgtcagttt ggcttcctga
      601 atcatggtgg agataatggt tgtaccgttg agaacatcta aaatgcttgc gatttttaat
      661 gtaggggcga cttgatgatg taaccagttc aatgttcgtt caaaagaata aggctctggc
      721 tgtgtggtta ggcgtaatgc tccacgattt ttaccgataa accattccca acgttcatta
      781 gttttccaat cactccgacg tttagtgtcg tctttatctg caaagcggat atagcgattg
      841 ataatttcaa aggctgtttt ttccgcctgt tgatgtgcca ataaatcttt catggcttgg
      901 gtggctcggt cattttttaa ccgaatttca aaccgattct ttacttctgc gtcttctaag
      961 ggaacatcat ttttcacgta ttgctcataa tctttctcat atatacagaa atagacttca
     1021 ctttttaatg agccaatata aagtgtgttt cccatgtatg gtttttcatc acggtgaacc
     1081 aattcaccac tgcgataatt tttaaagctt cgaaagacgg aaatgcattc ttcctgttta
     1141 catttctttg ccaaatctgg aatattcaaa atccctgttt tatcgttgat agctaaatcc
     1201 aagcgtttga aaataccttt atgttccatg caatcttgaa agaaatcata ccagctccga
     1261 tgttgagcca atagaaagtt ttcaaattgc cgacagcctt ttcctttcag ttctaataaa
     1321 acacctttac ttacatcatg agagactagg acaacaatat caccgaaacg atagtgttcg
     1381 ggataagaat aatagccaaa gtcttcgtgt agcatataat ccatattcaa acgtaaaacc
     1441 tcaccgataa tttgagccac atcattggta gggaatcgaa tccgtacata gtcgaataac
     1501 atctccaatg ggttatcggg attgaagcgt tccaaagcgt tcagtaaatc ttctttcacc
     1561 tgttgagtag ggactgcctt gcctgtttca atatccgata ggtaaggtct ggtaatatga
     1621 gaagcaaccg ccagtttatt ttgagaaacg ccataagcca gtcgcttttc tttcaattcc
     1681 ttaatccaaa ttttttcgtt ttccattcgc aaacctccaa tctaaaaaag atgtaaacta
     1741 tgacccttga gtttacacct ttcatatttt ccgcaatcct ttgtaccatc aacattggtt
     1801 gatgagaatt gcctgttttc agttgtattt gtgcccccct gttagataga gggggctaaa
     1861 ggattggcgt ggctaacgcc acaccaatca aagcaagtct gaacctgtac ctttcacttc
     1921 gtgcggtaca ggctcagatt gctttttttg aatgactttt cctatttctc ctaagaaatc
     1981 gtaccttttt ggtacaaggg gtgtataaaa ctcactaatc acactttttc ctgtatccac
     2041 gtaacctctt cccttgatgg gttttaagaa aaattgtttt tgtgtatcgc ttccgaacat
     2101 cataccaaag cccatttcag acatacgacc caaagccaca cggaagttaa agttgtcccg
     2161 aatcccatca ctaaaatatt ttgcgtctgg acgttgacag gcaagaatta aaaagaagcc
     2221 tgcttgtcgt cctaacatta caatttgttt gagtttatga ataacggtgg tactttcacg
     2281 acctagcata tccatcattg ccacatattc atcaaaaata agaaaattgg cagacaaacc
     2341 aagataagca taattttctc ctgttttata gttagggtgt tgtttcatgg tttcactgcg
     2401 atcaatcata tcttcgtaaa attgattgat acatgccaac atatcttctt tacggtaata
     2461 gacattattc atgaccgccc ctaagtctgc caaatcagca tttttagggt caagaatata
     2521 aagctttgca tttgtatgca ggagagattc aatcaatgtg agaataaaat aggattttcc
     2581 actaccagtg cccccagcaa ttaacatgtg agggagagaa tcgaactccc aatagaccga
     2641 ttccatcaat ttgacacgtc catcttgagc taccacgtct tcaatgccaa ttcgattgcc
     2701 aatagtatca taaagtagga catactctac ataagaatct ttcaacgttt tttccactaa
     2761 ttcacaatac aatcctgttt ctagtttgtt ttctaggtgc aaaagctgat cttgatattt
     2821 ccctaaggtg atttctacat gaatatggat tagtccatct ttcattcgat aatatatttt
     2881 aggaaaatgg cggattcgtt gtttggtttt cttgctgggt aaatctttaa agaatccctc
     2941 tgattgagtt tcttcggctt cataccaatg attttctaaa atcatacgag ctaacttttg
     3001 acggtgctct aactgtttta tagaatctat acgatagtga taataaagcc agctgataag
     3061 tccgcataca aaaagagcag tgagaatact gctccaaaag tatggcgtat gaagttgata
     3121 gaaaaatgta tctaactgaa ctgcttgcca gttcaccgat tgtaacgctc tccaatgaaa
     3181 agggagtagg acgactagaa aaactaccag caatgaaatt agtacaaaat gatagaccaa
     3241 gtgcttatcg ttggctttga ttcgctctcc tctgtcatga agcaatggca taaaaatcct
     3301 cctttccacc tatcaaaata attgtgtatt gtagatacga gcctgtttat caaaagttgg
     3361 ttgagaaatg gcttcatatt tctgataagc gattcgcttt tcaaagcgta aatttttttc
     3421 tttcggataa taatgacgtc gatagagagt gtgtcctaac agttttttcg cttcttttgt
     3481 caacggatag agatagcgaa acatgcgtcc attgattttc tcaatccctt tgtattcgca
     3541 aaatgcctgg gaaagccaat gctttttctc tacctgttca aagcgagcat tttcctctaa
     3601 taaaaggcga gcacttcgag ggtgtatctt ttcccatgac tgcttatcac gatagacact
     3661 tgtcttgaaa tacccgcagt aaaagaagtt ggacgcttga tagacatagc cacattttcc
     3721 ctcaattcca tctgccagtg tatataaaaa atgacagtcc gtatgttctt tcaaccactt
     3781 aattagagct gaaagagctt gactgccaaa ataattggtt tgattcattt ctggaagaaa
     3841 acacatcttt ccaatttcta aataatctgt ggtttgaagg gaactgtcgg gaaagagttt
     3901 gcgtatggtt tgtaggggtt gcgtacccca gcctaattcc acaacgccta ataatttttc
     3961 ttctgaaaaa atccctagaa agtatttaca caatctaggg agtatctttg aataatggta
     4021 ttgccgaata aaagaaatgg cttcatcctt cggaatttct ttcaaaatca atggtgtgtt
     4081 tgtcataagc aacacctatt tttcactttc ggacgcttct tttttatatt tctgatgagg
     4141 ttttgtctgt ccttttaata caagatcgtc tgctttcacg taccaatcaa cgtctgcccc
     4201 tcgaaaagta gccgtcgcta cggtatctgc cacaggattg attaattcca cttctgcgtt
     4261 gtagtcaaat tctttcaaag gaacagaagc ggggatactg acttgtatca tgcgtccttg
     4321 tccttttgat tttaagtcat aggtacgttc tttaatttct tcggaaaccg taccatcttc
     4381 attctggata cgaacctcac gacgcaaggc ggaaaatttc aatgtgccaa atgtctggtc
     4441 tttttcaatc acaatacctt ctgctaatct catatttttt cacctttcct ttcttatgct
     4501 ttcaccatat cgtctgcgtg cataatgtaa ttggtaaatc ctcgattgcc aattttataa
     4561 ccttctgcgg taatatgtgg gttgatgagt ttgacttttt cgtcggattc aaaatgtttc
     4621 tcgccagctt cggagggaag aatgacaaca atatcatctg ctcgttgaat cgttgagtat
     4681 aaattgtaac tacgagagag aatggttggt ctgccattta ttcgacgttg ttcaatgttc
     4741 ccttcaccag catattctaa ttgtccaaat gttttttcca tattgggaat gacatattta
     4801 agttccatat ttttacctat cctttctgtt tttatgtttg aatgaatctt atttgtgtga
     4861 tggttttaaa ggggggagag cgaccttttg attcttgatt ttttattttc ataagttcac
     4921 ttcctttcaa aattgggtaa aaaaatagac gctccgtact gagcgtccga ttcctactat
     4981 atttaaaaca acgactacct aggtatttta aatattataa tcaaattcta ttttatctaa
     5041 taattgagtg ggataaagca agtctccttt actcgtaaaa taacccacct ctgagaaaag
     5101 aaataaatta ttttttgctc ttgaacaaat tacgtatagt agttttctcg ctgaactaat
     5161 tcctttatca gaaacattaa aaatttcatt ccaatgagga attttcccgt ttagaagatt
     5221 aatagcgata acagtatcat actcatttcc tttaacttta tgtgctgtag taagtgtgat
     5281 accatctcga gatttaaaca tcttcttaaa actagataca ctactagata atgaattatt
     5341 ttttatccga tcttctactt cgttaataaa aaatagatag tgcttaaaaa tagacgggta
     5401 atttttctcg tcaatattaa gattaacaaa tatatgtttg gtaacaaatt tgtataaatc
     5461 aacaccgttg tcgtctccac tatacattct tttcacactg tttattagct gtaataaatc
     5521 taagtcaata aaataatctt taacttctat tccaaattca ttttttaat
//
LOCUS       DASJPC010000134         5531 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJPC010000134 DASJPC010000000
VERSION     DASJPC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.138"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..985)
                     /locus_tag="U4A13_002610"
     CDS             complement(<1..985)
                     /locus_tag="U4A13_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEN1780868.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="U4A13_002611"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="U4A13_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1780869.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="U4A13_002612"
     CDS             1810..2964
                     /locus_tag="U4A13_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1780870.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="U4A13_002613"
     CDS             3179..4147
                     /locus_tag="U4A13_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1780871.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="U4A13_002614"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="U4A13_002614"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1780872.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5531
                     /locus_tag="U4A13_002615"
     CDS             5177..>5531
                     /locus_tag="U4A13_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEN1780873.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1669 a         1078 c         1251 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc g
//
LOCUS       DASJPC010000135         5504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJPC010000135 DASJPC010000000
VERSION     DASJPC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.139"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            375..932
                     /locus_tag="U4A13_002616"
                     /pseudo
     CDS             375..932
                     /locus_tag="U4A13_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            990..3029
                     /locus_tag="U4A13_002617"
     CDS             990..3029
                     /locus_tag="U4A13_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN1780874.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3138..4289)
                     /locus_tag="U4A13_002618"
     CDS             complement(3138..4289)
                     /locus_tag="U4A13_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1780875.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4443..4973)
                     /locus_tag="U4A13_002619"
     CDS             complement(4443..4973)
                     /locus_tag="U4A13_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN1780876.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5072..5422)
                     /locus_tag="U4A13_002620"
     CDS             complement(5072..5422)
                     /locus_tag="U4A13_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN1780877.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         1991 a         1015 c         1037 g         1461 t
ORIGIN      
        1 tcaaattcca ttgcctaatc attaggaaaa gtctatcatg aactcgtaaa tagaatatag
       61 taataacctt gttaggtgag gctcctatac aaacataggc tgttgcccag aaacgtcgaa
      121 agtcgccaat gggtaaaaca gaaactgttg gattaaggct ttttccaata gctaagaatg
      181 ttttctttac gttgtatagt gctaaaactc ggacgatgag atgatgctag gtcagaattc
      241 ttgttcgaaa aggtttctga ccttttcttt atatttttgt gactgactta tttgatgaga
      301 caaactcgct cctcacctga caagtggatt gctgtgtgag gaggagatgc ggtgaaaaaa
      361 gatcgattgg tggaggtcgc tcggattcac ttctggttgc acaatacaac cagaaaacac
      421 aacaagcaaa actgatctct atcatgagag attcatatgt cgatattcca ggttacggaa
      481 tgaataagat aaatgcagct tactcttatg ggggagttga tttattaaat caaacattaa
      541 aggaaaattt caaatttgaa gctccctatt atgcaagtat cacatttcaa gattttatcg
      601 attgcgttaa tgaactgttt cctggtggag tcaagatcga tgcagaaaaa tctctagatt
      661 tagatggcgt atatataaac aaaggagagc aagtgatgga tggtaatact ttactccagt
      721 atgctcgatt tcgggaagac aaagaaggag acttcgggag gatcagaagg cagcagcaag
      781 tgatcaaagc tatctctcag cagctgaaag acgtaacttc aatattcaag ctacccaaag
      841 cggttgggaa attacttggt agtatccaaa cgaaccttcc agaaagtgtg cttctggatt
      901 gtggtatgga tttcctcaaa gacatatgtt agaataaaca ggtataaata gtgaaataaa
      961 ggaatgacaa gcaagagaag gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc
     1021 gaacaggcgc ttatattgcc ggcgcagtga tcttaatagc agctgcgggt ggcggttatt
     1081 tttactacca gcactatcaa gaaacccaag cagtagaagc tggagaaaag acagttgagc
     1141 aatttgtcca agctttgaac aagggagatt ataacaaagc tgcagaaatg acatcaaaaa
     1201 aggcggcaaa taaaagtgca ttatctgaaa aagagatctt agataaatac cagaatatat
     1261 acggtgctgc tgatgtcaaa ggacttcaga tatcgaatct aaaagtagat aaaaaagacg
     1321 attctactta cagtttttca tataaagcaa agatgaatac ctcattaggt gaattgaaag
     1381 atctttctta taaaggaaca ttagacagaa atgatggtca aaccacgatc aactggcaac
     1441 ccaacttggt ttttccagaa atggaaggaa atgacaaagt aagcctgacc acacaagaag
     1501 cagcaagagg gaatattata gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa
     1561 aacaattagg agtcgttcca agtaaacttg gagatggagg cgaaaaaaca gccaatatca
     1621 aagcgattgc ttcctccttc gacttaacag aagatgctat caatcaggcg atttcacaaa
     1681 gctgggtaca acccgattac tttgtcccat tgaaaatcat tgatggagca acgccagaac
     1741 ttccagctgg agctaccatt caagaagtag acggcagata ttatcctttg ggtgaagcag
     1801 ctgctcagct gattggttac gtgggagata tcacagcaga agatattgat aagaatccag
     1861 aattaagcag taatggaaaa atcggtcgct caggtttgga gatggctttt gataaggatc
     1921 ttcgtgggac aacaggtggg aaattaagca ttacagatgc agacggtgta gagaaaaaag
     1981 ttctgatcga acatgaagtt caaaatggca aagatatcaa attgacaatc gatgcgaagg
     2041 cacaaaaaat agcttttgac agtctaggag gaaaagctgc atcaactgtt gcgacaacgc
     2101 caaaaaccgg tgatcttctt gcgcttgcta gctctccaag ctatgatcca aacaaaatga
     2161 caaacgggat ctcacaagaa gattacaaag cttatgaaga aaatcctgaa catccattca
     2221 tcagccgatt tgcgacaggt tatgctcctg gatctacgtt taaaatgatt acagcagcaa
     2281 tcggtctcga caacggcact atcgatccaa atgaagtgtt gacgatcaac gggcttaaat
     2341 ggcaaaaaga cagttcttgg ggatctttta aaacaacgcg tgtcagtagt gatgtatcac
     2401 aagtagactt aaaaactgct ttgatctatt ccgataatat atatgcggca caagaaacgt
     2461 tgaaaatggg tgagaaaaaa tttcgtacag gcttagataa attcattttt ggtgaagacc
     2521 ttgatttgcc aattagtatg aatccagcac aaatttctaa tgaagatagc tttaactcag
     2581 atatcttgct agctgatact ggatatggac agggcgaact tctaattact cctatccagc
     2641 aagcagcaat gtattctgtt tttaccaaca atggcacact tgtctatcct aaattgattg
     2701 cagataaaga gacaaaagat aagaagaatg tcatcagcga aacagcagta caaacgatcg
     2761 tgccagatct gagagaagtt gtgcaagatg taaatggtac agcacattct ctttctgctt
     2821 tagggattcc gttggcagcg aaaactggta cagcggaaat caaagaaaaa caggatgtaa
     2881 aggggcaaga gaacagtttc ttgtttgctt tcaatcttga taaccaaggt tatatgatgg
     2941 tcagcatgtt ggaaaataaa gaagatgatg attcagcaac caaacgagca tcggaactat
     3001 tgcaatacct caaccaaaat tatcaataaa aaattgcttt ttaagtacag taaaaggact
     3061 gtggcgttat cgtcacagtc cttttttcaa ataaacggtt ttcacgatgt ctctttcaca
     3121 acttcttttt gatttactca cacttcataa actttttttg taaaatattt gagaacaagt
     3181 ggagaaataa gtgtacttaa taaaatcacg ataactaaag gtgaatagta ctgtggttcg
     3241 atcaattgac tttgctgtcc aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc
     3301 ataccagcac cgaccatcaa tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa
     3361 taacctccaa gcaatttggt caaaatcgct acaagcgtca atatagaaat aaagagtagt
     3421 tgttcactaa agtcagaaaa atcaacttct aatcctacac tgacaaaaaa tactggtata
     3481 aatactgcat atcctaaagc ctctacattg tggtatactt cttctttaac ctttgtctga
     3541 ctgacagcta tcccagcaaa aaaagcacca atgacagagc taagcccaac aagatccgct
     3601 acgtaagaca ttcctaaaca aataactagc gatacgatga taatagaaga atttgcgtac
     3661 actttttctg caaatgaaag taggaaaggc gcaatccatt tgactaataa gaaaataaaa
     3721 acaaaataaa ttagctgttc aatcaaaaca agcggtaaag aagcattgct agaagcttcc
     3781 cccgttagaa aagataggct gaaacttaga actaaaacga ctaaaatgtc atctaccaca
     3841 gaagcaccca aaattgttga tccttctttt gtattcacta cgtttagttc ttttaatact
     3901 tctactgaaa tactgacaga agttgccgca agaataatcc caaagaaaat cgcttcattt
     3961 cccgaaactt ggaatgcttt gcctgtcaac cagccaaaaa caaccgggaa gatgatccca
     4021 agcaaagcaa cgaacatacc aggtcggaaa tattttttca aaagagataa atcactttcc
     4081 agaccagcta aaaacattaa taagatcaca ccaatctccg aaaagtcatg gactagaata
     4141 tcaggtttga cccaattcaa tccagcttgg cctagcaaaa caccaacaag taattgtcca
     4201 atcactgctg ggatacctaa tctccgcgag atatgtgagc caatcgttgt tgaaatcaat
     4261 atcaggcata aaataccaat aaattccatt ctcatcattc ctttgtataa aatatagccc
     4321 gaacttagca taacatacat tattgaaaaa ataggaaaat atactcacaa aaaaaccaac
     4381 aatccaaaag atttttttgg attgttggtt tttatacggt ctattttaat aactaaaaat
     4441 gattaacgta tagtcatttt tcttattctg atgaaattga taatctcctg gatgcccatg
     4501 aaaatcaaaa taaagccgaa tatttggaat agaagcatca aacttttgaa tggattgaac
     4561 aataagacga atccgccaat caacaacaga atactactat aaacactcca acttttagct
     4621 tttgtattca tgctgaagaa caactggaac aagctgttga tgataacagc taatccaaga
     4681 agaatcggca ggatagaaac gattgcaggt gcaaaagcat agacgatcag agctaagatc
     4741 aggaaaaaaa gaccactgaa cagaccgata ctccaagaac ctgttttcct tttaaaacgg
     4801 taagcctcaa aaagattcaa caacccaagc aaaacaagat aggcggtgat cagataaccg
     4861 ataaaatgaa aaacagcaga agggtttagc acaatcgcaa tcccagaaat aatataaaca
     4921 gcagctctta ataaggcgta tttttgaata ttttgtttaa tcgtctcaaa catataagaa
     4981 aacctccttt gacttatttt aacgtgaact agtcatttta ccaaattaaa tgttacaaaa
     5041 tgagcgaagg agaagaatat ctctggaaaa atcaacttct gaggatcttt tccatttttt
     5101 tgccttttgc tagctcatcg atcattttat ctaaataacg aatttctttc atcaagttat
     5161 cctcgatgtc ttctactcgt acaccacaaa caacacctgt aatcaaggaa cgtgaaggat
     5221 tggggacagg ggcttccgca aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt
     5281 ctagttctgt ttgggaataa cccgtcagcc aacaaatgat ttcatctact tcctctttta
     5341 tacgatcttt tctctccgct tttgctacat acataggata aacgcttgca aaacttgtcg
     5401 taaaaatacg attttgcgcc ataatcagtc acctttcttt ttttcaaatt atttactact
     5461 tatagtatat aagaaaaaga gaagaaaaag cgattattca aaaa
//
LOCUS       DASJPC010000136         5458 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJPC010000136 DASJPC010000000
VERSION     DASJPC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.140"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1038)
                     /locus_tag="U4A13_002621"
     CDS             complement(<1..1038)
                     /locus_tag="U4A13_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1780878.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKE"
     gene            complement(1082..1846)
                     /locus_tag="U4A13_002622"
     CDS             complement(1082..1846)
                     /locus_tag="U4A13_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN1780879.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(1874..2572)
                     /locus_tag="U4A13_002623"
     CDS             complement(1874..2572)
                     /locus_tag="U4A13_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN1780880.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2584..3363)
                     /locus_tag="U4A13_002624"
     CDS             complement(2584..3363)
                     /locus_tag="U4A13_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN1780881.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(3379..4323)
                     /locus_tag="U4A13_002625"
     CDS             complement(3379..4323)
                     /locus_tag="U4A13_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN1780882.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4369..5148)
                     /locus_tag="U4A13_002626"
     CDS             complement(4369..5148)
                     /locus_tag="U4A13_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780883.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1495 a         1137 c          929 g         1897 t
ORIGIN      
        1 ttctttcatc aactctgctt tgcgttcttc tgcatccata tacatactac ggaacttata
       61 catcttaaat gtcttaccat tttgaccaat acgttcttgt gcaaagaaaa tcggccctgg
      121 tgatttgatg taaataactg gtgctacgaa gataaaaata acacctgttg caatacatcc
      181 aataattcca gcaagaatgt caatcgttct ctttataaat gcttgcctca tcgtcataac
      241 attaatacta gatgtaataa cagtataatc accaactcgg ccaataaatt gttttccacg
      301 tgtactatca gacacttttg taagattcat atgtacagca agtcccattt ccgaacatct
      361 ggcgattaat tctttgggat atggaacact ttcgtcaaca ttgatgaata cttcatctac
      421 ccaattctgt aaaagatatt ccgctgcatt atcagcattt gcaactactg ggacacctcc
      481 gatggtagtt ccatacatat ctctgtctac tacaatcaca ccactaagtt tatacatgcc
      541 ataattcttc tctttaagat tacgaactac attttttgca atcttttgag atgttacaat
      601 aactaacgat acatcttcac cgtttttcat gcggtgatgc aacaaccctt tccaaagtat
      661 acgaattata tatgttaata atccatacac cacccccatt ataaataaag aaactctgga
      721 ataatcattt ccatcctgca ttgtaataag gtataaactg cacacaagta acagcataaa
      781 agattgcttt atagaagcta cagcctcttt ataatatcct ctttttaaaa catttttata
      841 tgcctcaaat aaaaatataa tcgtaagatt ctcaagttct ataaaaattg ccatgtggcg
      901 ataaatagga atcacatacg gattccagtc accctgtcgc attacatatg atatataaaa
      961 agcaagctgg acacaaaata agtctaataa aataaaatca atatgtttag accaaccact
     1021 tgagtttttt ctatacatct ctcactctct cctcatttcc tttttatcta cgaataaaga
     1081 actaaaataa accaaacttc ttctttttta cctcttcata gattggataa ttgatctcat
     1141 ccccgttgat cagatccttt gcgacttgtt tcatctgtac tactttttcc ttaccaaact
     1201 ccttggcaat ctgctcatag cactctttta agtaaaaggt gcgcttagag accccgtgtg
     1261 catcagaagc agccatctgc acgagattat gtttgaccat tgttttggct gttttctgga
     1321 tatctttgcc aaatacacca acataacttg gcgcagtaag ttgggccaaa catcccatat
     1381 ctaggaacgg aagcaaatga tttgggtctt ctcgaaaatg cgcgttgcgt tctggatgga
     1441 cgatcactgg tatctttcct agttctaaca actcaaaaaa aagccgttct gcatataacg
     1501 gcacttccag tgtcggaaat tcgatcagaa gatatgtatc atctaaatct gtaaataaaa
     1561 tttcatctcg gtggatgtct tcgatgagcg ttccggtaat acgtacttct tgtccttcca
     1621 ataacgtcaa aggcaactgt cttttctcta attcggcttg taacgaagcg actagagaaa
     1681 tgacttgtga tttttcattg ctgtattttc cattattgtg gtgtggtgtg cataaaatat
     1741 gcgtaatgcc ttgctgaatc gctttttcag ccattgcaat acttgcttct aagttttctg
     1801 ccccgtcatc gattcctggt aggatatggc aatgtagatc gatcatttct cttgtctcct
     1861 ctcttcttat tccttaattt ccgtagtaat aaccagcatc tttgctgtgt tctgctccgt
     1921 tgtatacgac acctaagact cgtgcttgta ccatatctaa cagttctttt gctttagtca
     1981 atgattcttt tcgtgacgta ttttcccgta cgaccaataa ggtgccatcc gtttttgaag
     2041 acatgatctg tgcatctgtt acggcaacga ctggcggcat gtcgaagatc actacatcaa
     2101 atagttgtct tgcttctgct aaaatctgat ccattcttgg cgaacttagt agttctgatg
     2161 gatttggtgg tttaggtcca cttggcagaa tcgacaaatt gtcgactggt gttcgttgga
     2221 tctcatctgc tacacttcct gatgaactaa gaacagtact taatccgctt gcgttgttca
     2281 acttgaacgt tttatagatg acgggcttac gtaagtctgc atcgactagc agcacccgtt
     2341 gaccagattt ggcaaaaaca acggcaatgt ttgctgcagt ggtcgatttc ccttctccag
     2401 gtcctgatga agtcacaaca atcgttttga tttgctgacc agctgcggag gcaaactgga
     2461 tattcgtccg aatcgtacga tattgttcag cgattggtga agagggattg gtgagtgtga
     2521 ccaaactgac tgcatgagtt tgggctgctt tttgtttctg tgttcgtgcc attttctttc
     2581 ctcctagact cttgagcggc ttctgcgtga tggcgctgtt ttatcgtttg tagcttttgg
     2641 ttgtgtctga gctggtcctt ttttacggat accagcattc aattctttcg ctgacatttc
     2701 tggaactgtt cctaagattg ggaatcctaa tgtatctgtc acatattttt catctttcac
     2761 tgtccgatcg agtaattcca ataagaatgc cagacctaca cctaccatca ttcctaacat
     2821 taagccagtc attaaattca atttattatt tggcgaaaca ggtgtcgtat tggcaactgc
     2881 acctgaaatg atcgagattt tatcaacatt catcacatct tttgcgttct cttggaaaac
     2941 ttgtgaagtt gtgttagcga tctgttgagc agccactgca ttcgtactta ttgcctgaat
     3001 cgagaacatt tgtgaatttt ctgcctgatt aactgagatg gcatctttga tttgttcagc
     3061 agtcattttc acattgtctt ctgtttctaa acgatctttg acttcattga gcactaaatc
     3121 actgacgatc atatctttat acgtattgat catttgcagg ttcgtattga catcattgac
     3181 atttgcagtg tctgattgtg gcagtgtcac gatcaattgt gcttgtgaac tgtattttgg
     3241 tgtcaagatg aaaaatgtta cgattcctga gatggccaag ccagcaaaca ttgagatgat
     3301 gattattacc aagtgtttgc gtaatgtttc aaaaatttct ttcaagctga ttgtctcttc
     3361 cattttttct ccactttcct atttattctc tagattcatt tgatctttta attctttttt
     3421 cacacgatca agttcttgat cactgacttt ttgataggag atattatctt gcatgaagcc
     3481 ttctccttgc agctgatctt gcttaatcgt accaaatgcc tcccgataat ctaaagcaat
     3541 ttttttcatt tcatcaaagt tcatgtctgt tttcatattc gactctagag cattcaaaac
     3601 ggattgatag ttgctgacac cgtttagact caatactttt ttcgctacgc cttctacgat
     3661 ctttctttgg cgcccttgtc taccgtaatc accatttggg tcttcataac gcatacgaga
     3721 ataagataat gcctgatccc cgttcaatgt aattttaccg attggaaagt catagccatc
     3781 ttgtgagaaa gtgaggtcat tattgacttc gataccacca actgcatcaa cgagttcctt
     3841 tagccctttc atattgatag agacatagtg atcgattggg atgtctacat atttttgtac
     3901 ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg tggttgatct tgtcttttgt
     3961 gtcacgacct acgatgtcga catacgtatc acgaggaatg ctgacgatcg ttgtctgttt
     4021 gtcttcagga ctgactgtgg caaccatcat cgtatctgat cttccttgat cgacccgacc
     4081 taaatctcca gtatcaatcc ccatcaatag aacagagaaa gattcttgtt tgtccagatc
     4141 aactggtttc gttcgttctc cttgggttcg ttccactgat tcgtaagttt tctcaataga
     4201 atgtgagaga tccagatata attttgctcc tactcctaca attgctaata ctaatacgat
     4261 aagaactgct aaaataatca gaaatatttt tttccctctt gattttcttc ttcgtcttgc
     4321 catagtttcc tccgtttgct aaccttgctt gaatcaacgt tttaactgtc aatagcttat
     4381 attgctatag cccatttgct cagcaacagt ttgtgctttt gttgcgtatg ctgcgcttat
     4441 tttttcattc cggatctgct ctatttgact gagcaccgtc aaatattgat cgtaagtagc
     4501 ggtggcagtc acttgaccat cctttaacat cgtatcgaat aactgatcta gttgcgtgac
     4561 ttgcgcgatc tgaatatcag cgaatcctag atattgcata acaaccgttt tccaaggact
     4621 atctttaaaa aagctcatat tttcacgtac acgagcgaca tctgcacttg tcaccttttc
     4681 tttgatgatg acatcgtcta cagcttgctg ccagtttgat acatttttag taaataaatc
     4741 gttgacagct gtctggatct tcagttttga ttctgcacta tctaactgtg tcacgattgc
     4801 tttcttctca tcttggattg caacagcttc tttaggcaat tctgattctt tgatgtcgta
     4861 atcatctgct gaaagtttga cagaggatag cttatttgac aaacttgtga ttgtttcttc
     4921 tgttatttca gggtttagaa aaattttttc ttttgtacta tacagcttgt ctacttcttt
     4981 tgataaagaa gtcaaggtgt ctttttgatt ggtgatggca gattctgcat aattgatccg
     5041 ttgctgatgt tgccgctgat tattgatatg gtatccaaac aaacagatcg caatagcacc
     5101 tgcacctacc gccacagcag tcaatgactt gacgaatggc ttttccatag attcactcct
     5161 aagtattgat gatagataaa ttaagatata attcacttac ataaaaatga cagcttttta
     5221 tttaaaaaaa cataattaaa ctaatatatt cacagaaata tttgtaaaca agttttccct
     5281 atataatcat accacaaact gttttcttga ctagggcaat ttacgattta aaagtaaata
     5341 aaacattaat aagataaaaa ttaatcgatt tcatttttat ttccataatt ttcataggtg
     5401 tttttaaaag ttacacaaaa aatagaataa caaaaaaatt aacattgaat taataata
//
LOCUS       DASJPC010000137         5342 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJPC010000137 DASJPC010000000
VERSION     DASJPC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.141"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(61..279)
                     /locus_tag="U4A13_002627"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="U4A13_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="U4A13_002628"
     CDS             complement(545..739)
                     /locus_tag="U4A13_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780884.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="U4A13_002629"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="U4A13_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="U4A13_002630"
     CDS             2091..3131
                     /locus_tag="U4A13_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN1780885.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="U4A13_002631"
     CDS             3955..4287
                     /locus_tag="U4A13_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN1780886.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="U4A13_002632"
     CDS             4906..5247
                     /locus_tag="U4A13_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN1780887.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1771 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaaacata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag aggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgatgatt aataaaaagg tagtattacc ttaggcattg agtaaggaat
     5341 tt
//
LOCUS       DASJPC010000138         5247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJPC010000138 DASJPC010000000
VERSION     DASJPC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.142"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="U4A13_002633"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="U4A13_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEN1780888.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="U4A13_002634"
     CDS             1402..2802
                     /locus_tag="U4A13_002634"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEN1780889.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="U4A13_002635"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="U4A13_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5247
                     /locus_tag="U4A13_002636"
                     /pseudo
     CDS             4800..5247
                     /locus_tag="U4A13_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1675 a          850 c         1001 g         1721 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DASJPC010000139         5154 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJPC010000139 DASJPC010000000
VERSION     DASJPC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.143"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2377)
                     /gene="esp"
                     /locus_tag="U4A13_002637"
     CDS             complement(<1..2377)
                     /gene="esp"
                     /locus_tag="U4A13_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN1780890.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKD"
     gene            complement(2994..3428)
                     /locus_tag="U4A13_002638"
     CDS             complement(2994..3428)
                     /locus_tag="U4A13_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780891.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3445..4650)
                     /locus_tag="U4A13_002639"
     CDS             complement(3445..4650)
                     /locus_tag="U4A13_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN1780892.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
BASE COUNT         1633 a          938 c          693 g         1890 t
ORIGIN      
        1 cgtctttcac tgcaaagact gttgttccac tttcaatact tgttccttcc cctgcggtga
       61 aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa atatctttac
      121 ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca attttaatag
      181 gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg aatgtaacaa
      241 taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca tctgaaattg
      301 aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt ccactttgaa
      361 ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc atagcttgag
      421 aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc ttaacagtta
      481 caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc catgaatact
      541 ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct ggtttaaatc
      601 caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg gttgaacctt
      661 cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct ttcgcgatta
      721 atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt ggagccccat
      781 ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta tcaacaagtt
      841 gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta actgttgtgt
      901 caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg tctccgctct
      961 cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa taacctgttc
     1021 cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca aagatgaaat
     1081 cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct tctaagattt
     1141 catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt ggacttgcat
     1201 tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg ccataatttt
     1261 catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat tcaaacgaaa
     1321 catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct acatatggaa
     1381 ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg gaagatgaat
     1441 atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca ttagcacctg
     1501 tggatcctga accatctttt gtttgagtat taggtttctc atattttgca cttactgatc
     1561 catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag aaatcatctg
     1621 aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca tcgtcttgat
     1681 gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct atgcctatct
     1741 ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga gcggtttgcg
     1801 tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa ttgaccttcc
     1861 aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt ctagaagatc
     1921 caattccttt ctttggatcg acctgaattt tttctacata tttagctaat cgttcatcta
     1981 attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt ccgtaaactt
     2041 caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga ctaaattgta
     2101 aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta ttccccgaag
     2161 ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg cttggagaaa
     2221 tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca ccaatcaaaa
     2281 cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt ttctttgttt
     2341 tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac tcccttaata
     2401 attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca gaataggaat
     2461 cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta caacaaaaaa
     2521 agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca gcagctgaaa
     2581 acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta tgcgatgaag
     2641 catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa ataacggaat
     2701 aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag gtgacttcaa
     2761 attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg gtttcacagc
     2821 atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca ttttttcact
     2881 cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat tgattctttt
     2941 tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa taattaattg
     3001 ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc tgaaaaagtt
     3061 agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa tttttgataa
     3121 tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc atctagctta
     3181 atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat agattctcta
     3241 gcttcttcac ataaactttt tactttatta attactactt tgtcatattc atttaacaca
     3301 ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc agaaatttcc
     3361 caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg taaatgatat
     3421 agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca ctaaacctaa
     3481 ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa agtgtgaaga
     3541 atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt tcaacattat
     3601 tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat gcatttcatt
     3661 cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta tttcctttac
     3721 agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa ttaagggaga
     3781 aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc cagaatccct
     3841 cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca aagcatctct
     3901 actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg ctaacttttc
     3961 aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa cacttggaat
     4021 tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc catttttaac
     4081 atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt aattttgtga
     4141 taacagatta tctattttga tttgctcgat agttttactt agattttctg tatattcaat
     4201 taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa ctaaaatcag
     4261 aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat tatagagtcg
     4321 ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa aaactttatc
     4381 aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca tgtatagaag
     4441 aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt taatattatc
     4501 agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc gatactcttt
     4561 tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa catgaagaga
     4621 ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt tctacatcat
     4681 taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa gctaacttac
     4741 ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa agtacatttt
     4801 atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata tctagttcaa
     4861 agacaataat tattatgatc cgttattatc taagcactat acagataata ggaatttttc
     4921 gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata cgtgtctatt
     4981 cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac aatctataca
     5041 cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg taaaaagctc
     5101 aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat gaat
//
LOCUS       DASJPC010000140         5042 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJPC010000140 DASJPC010000000
VERSION     DASJPC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.144"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..182)
                     /locus_tag="U4A13_002640"
     CDS             complement(<1..182)
                     /locus_tag="U4A13_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN1780893.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="U4A13_002641"
     CDS             517..951
                     /locus_tag="U4A13_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN1780894.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="U4A13_002642"
     CDS             974..1294
                     /locus_tag="U4A13_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780895.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="U4A13_002643"
     CDS             1435..1626
                     /locus_tag="U4A13_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780896.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="U4A13_002644"
     CDS             complement(1697..1954)
                     /locus_tag="U4A13_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN1780897.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="U4A13_002645"
     CDS             2112..2375
                     /locus_tag="U4A13_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780898.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="U4A13_002646"
     CDS             2399..2587
                     /locus_tag="U4A13_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780899.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="U4A13_002647"
     CDS             complement(2670..2855)
                     /locus_tag="U4A13_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780900.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="U4A13_002648"
     CDS             3070..3441
                     /locus_tag="U4A13_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN1780901.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="U4A13_002649"
     tRNA            complement(3591..3663)
                     /locus_tag="U4A13_002649"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="U4A13_002650"
     CDS             complement(3709..4137)
                     /locus_tag="U4A13_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780902.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="U4A13_002651"
     CDS             complement(4197..4955)
                     /locus_tag="U4A13_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN1780903.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1757 a          784 c          987 g         1514 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcagttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 ga
//
LOCUS       DASJPC010000141         5039 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJPC010000141 DASJPC010000000
VERSION     DASJPC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.145"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            413..619
                     /locus_tag="U4A13_002652"
     CDS             413..619
                     /locus_tag="U4A13_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN1780904.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(821..1822)
                     /locus_tag="U4A13_002653"
     CDS             complement(821..1822)
                     /locus_tag="U4A13_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1780905.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1827..3740)
                     /locus_tag="U4A13_002654"
     CDS             complement(1827..3740)
                     /locus_tag="U4A13_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1780906.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3908..4414)
                     /locus_tag="U4A13_002655"
     CDS             complement(3908..4414)
                     /locus_tag="U4A13_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN1780907.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4574..5014
                     /locus_tag="U4A13_002656"
     CDS             4574..5014
                     /locus_tag="U4A13_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN1780908.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1391 a         1008 c          769 g         1871 t
ORIGIN      
        1 tatttttgtt tcttattttc taaaaaatta aaaaaaattt tggtgaatta agtatttcct
       61 tgataacact ggcttttggc ctcattttag gttatcattt gtaattttat ttcacagttg
      121 atatgattgt tatgtaaaat aaaaaagttt aggatacatc gaatttttgg ggaaattctt
      181 ttgggggtat cctaaatctt ttctattata ttcccatatt ttctattatt ggtctagacg
      241 tatgatggta aactttgtac ctatctgatt gctgaatcat taattgctta tcattagcca
      301 tctttgtttc aagttcgcta gatgatcatt ttattcacat cctaacaaac ttgtcacatt
      361 tatagattct taatctattt atcatacaat tataaaaaag gaggtgctgc ttatgaaagc
      421 tatccatatt tctttgcttt ttttcgctag tgcattgatt ttatttgcta ttcatctttg
      481 ttcccagcct tatatcaata acaacggaat attagtggaa ccagcattct tttgcttacc
      541 actcggcttt ttgtcccttc ttgcctccgc aggcttcgga tttgttgctt tttttaaacg
      601 atcaaaacaa caaatctgac ttataaaatt tacgtaaaag agatcgtgac aataatcgtg
      661 tgatattccc aaatttcgaa taaaaggtgc cccttgccat ctctaactcc tcagtcacaa
      721 gttacaatat tcataaacca tgaaaagctt gtttattgaa attgctcctt gtgacttgga
      781 gctaagtgac agattacaac ctcttttgca tcataagact ttactccttg aaaaacatta
      841 ctaacttttg atatgtttct tgcgcttgtt tattgctgaa atcaaactga tattcatggt
      901 tcaaaacagc atttttctcc gcataatcta cttttgtgac tttcaccccc gctttctcaa
      961 atacagaagt tacttcttcc atttgtttcg taaacgtatt tgtatttcca tccgttaaga
     1021 atgtgttttg cgtccaacga tccgcatgtc tgaccaaggc taaacttttg accttctctg
     1081 attgttcaaa gtccttatta tcaaagtaag cccatccaca tttttcataa agaaagctag
     1141 agattttatc acttacttga gtaagtgcag aaggatccaa cagaccagac aaagaaataa
     1201 acttcgtaat ctgcttgttc tccaaagtcg gatcaagatg atcagctttt ttaatttctt
     1261 cattttgaag tgctaacaaa tattctccac taatttgtgc acctgccgaa tttccaccaa
     1321 tcgaaatatt cgaccaatct gcttgatctt ttatctcttt tttagctgct tgaatggctt
     1381 cgttcaattg gataactggt gtaggatgtt ttgcttccgg tgatcgttca taatctatat
     1441 ttacgatgat ttgctgcgtg tctgaacata gatattccaa ataatcacga caatcatttt
     1501 tatcccctcc tacatatgcg cctccatgca tccagaaaag aatcggtaat tttttctttt
     1561 ccactttcgg tgtgtagata tccatattat tatttttgta ctttgaagga taggatattt
     1621 ctttttttat atcgatttgt tcgatttttt gctggaaatc atctggtcgg gcatatactt
     1681 gttctttttc acttgtgtca aattgttttc taacaagcaa gctaatcggc tttggtgtga
     1741 tttgattaat caaaaataca gtgactaaaa taactacgat gataactggt actgcaatca
     1801 ctattcgctt gaaccatttc attcatttac cctctctttc ttcgccaaat ccatgtgata
     1861 atcgtgctaa cagataaaaa tccacctgaa taaatgagaa gcgaatgttt cttttctcca
     1921 gttttcggca acattctttt ttttgcatct gtcttttttt gtactttcac tgtcatctga
     1981 atcggttgcg tatcatcact tgattgatcc tctcgaggtt cattctttcc cttattgtct
     2041 gcattctctg tcggatcttg cttttttggt ggagctactg ggattttttt gtaatagaaa
     2101 ataatagttg gtatcgtctc attaaaaatc ccttcagtcg gtccttcaac atttgctaat
     2161 tcgtatcctt cgaatgtctt ttgctctgtc atataatttt ctccataagc tccgtaaagc
     2221 tggatttcgt cctggtcttt gagtagactt ttattgtttt catccacata ctttacgttt
     2281 atctgatcat ggaacactac tttcaaaaag tccgttacat gtttcgtgta ggcagcatgg
     2341 tcaacttcct gtgcctttcc atgccctgct ccttctacta ataaaagatc tttataccct
     2401 cctgataact catataattt ttgcgcgtta gacacaggta ctacatcatc tgctgttcca
     2461 tggataatca atttaggcaa tgaatctgac attccaatcg taggtagagc agcttttgta
     2521 tccaaatgca tctcctgatc aaaatagtat tgtttcaaaa ggcccataaa taaatcttca
     2581 tgctcttgat ccccaatgat gtctaactga ttttttatgg catccggtag tgcgggggct
     2641 actgtctggc tgtacgtaaa acgtagttgt tcatcaattg atgcaaatcc gcaattttca
     2701 ataattcctt tgacattgac aggaatatct ttcgctaata cagacatcat cgtagctgct
     2761 cccatcgaac caccataaag taaaatcttt tgagatggcc gctctcgaac ttcttgattt
     2821 atccagtaaa ggacgtcatc tgactcatat tgtccatagg tcacataatc gcctccgcta
     2881 ttaccagtcg ctctggaatc aacaaataat aaattatacc cggccttata aaaatcattg
     2941 tattcttctg taacgatccc gttattccaa ttccctcgaa atccgccatg gatcatgact
     3001 gttttatctg aaccttgatc gacataatat gcatatacat ccatcatttt gccatcttgt
     3061 ttgtttagta cagtttgatg aagttgtttt gtatttggat agaagcctgt gttttctgta
     3121 ggcgttgtcc catcatctgt acctccaagc atcgacatca acacactgtt ttttgcaaaa
     3181 gcgatatcgt acaatagata agtagcaatc agatgtttga taatctcttt atctacacca
     3241 taatcattaa cagcaagctc agtgactaat aactctatta attttgactt gatgtcttct
     3301 gttggtccat taagtatttc tgtcatctgt ttaccgaagt aattcaaata ttctttacca
     3361 ttgaaatttt cacctttacc gatatcatca ttttctgtcc cattctcttt gtcacttact
     3421 ttagcagaag aggattcgtc gtttgacatt tttgtagctg ttgcttcttc tgtttgttgg
     3481 ttatctgtgg tagcggtcgt ttcttgatct gtgctagtaa cagatgtctc aaccgttgtt
     3541 tcagtatttg tttctgtttg tgaagtactc gatttcgttt catttccagc atcagtagtt
     3601 tctgtggttt ctacctcccg agattccgat tcggaagaaa tgctctctcc atatactgtc
     3661 cattgcggga cgcttgtact tacagcaaat aagatgacca acgctccctt taatacttgt
     3721 tttgttctca agctattcac tcctttttat gtttctagga gaattatatg taaacgcaaa
     3781 cattttttat acaaaaaata aatggtttat cccataaaaa tagaacagga ttattttctc
     3841 attttacgta ataaagcaga atggataaac cagtatccat tctgcttttt ttaaacgaag
     3901 aaacaactta ttgctccaaa aatgtcagaa atcgtttatc ccaaattttc tcgtaaaaag
     3961 cgattgtttc ctttgctttc ataaaaggat tatcataagt tgtcgttgct cctctacaca
     4021 tcataagctg gtatcctaat ccatgagcaa acttcacagt cgtatctata caatactctg
     4081 cttgtgcccc acataattcg atcgaatgac agtccaattc agttaataaa acactcaaat
     4141 tagtcttata aaatgaattc gcatgggttt ttctcacaaa ataactgtgc tcaggaatag
     4201 ataattccga caatagctcc catggttttg tttgtggcac taattgagca tcttcgtgtt
     4261 gaacgaaaat aactggtttc ccaatcgttt cgtatgtttt gattctttta ttcactaatg
     4321 ctgtcactgt ttctaattcg tgtatcggcg aagaatcaaa acatacccct ttttgtaaat
     4381 cgatcactac tattgcgtcg gctgtgtttg acatacttgt gccttctttc ttttttcttg
     4441 atttaatagt atttcttatc tagctagttc cattatacgt gacaacattt tcttatttta
     4501 cacctctaaa tggcactttt cgactatttc ttcatgttat tatttcataa atacttagct
     4561 attggaggaa tgtatgatga ttaaattgta tacaggttct ggctgctcgt cttcaagacg
     4621 cgcaaaacgt tggcttatcg tccatgatat accgtttatc gaacagaact taattcgtca
     4681 accactaacc aaaaatgaat ttatccactt actaaaattg acaaacaacg gcgtgaccga
     4741 aatgctctct actcattcta ttagttataa aaatcttgtt cacagaatag atgaactcag
     4801 tttgagtgaa ctatatattg agattcgtac atccccttct ttattgaaaa aaccgattat
     4861 ctacgaccaa tctgcaaaac ttttgactgg atttagcgaa gataaaatca ggatgttcct
     4921 ttctaaaact aaaagaaagc aagagctcaa cgaactcttg ctttctggtc atcttgtcga
     4981 ttttaaagaa aataatcttt ttgcaatgtc ttaattttgg cataaacaat ctgcttttc
//
LOCUS       DASJPC010000142         4930 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJPC010000142 DASJPC010000000
VERSION     DASJPC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.147"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            35..694
                     /locus_tag="U4A13_002657"
     CDS             35..694
                     /locus_tag="U4A13_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1780909.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            713..2296
                     /locus_tag="U4A13_002658"
     CDS             713..2296
                     /locus_tag="U4A13_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEN1780910.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2431..3009
                     /locus_tag="U4A13_002659"
     CDS             2431..3009
                     /locus_tag="U4A13_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780911.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            2955..3473
                     /locus_tag="U4A13_002660"
     CDS             2955..3473
                     /locus_tag="U4A13_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780912.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3481..4521
                     /locus_tag="U4A13_002661"
     CDS             3481..4521
                     /locus_tag="U4A13_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN1780913.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            4770..>4930
                     /locus_tag="U4A13_002662"
     CDS             4770..>4930
                     /locus_tag="U4A13_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1780914.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         1818 a          567 c          964 g         1581 t
ORIGIN      
        1 aaatattctt aaaaaaaaga gtataggatg tcgtatgaaa atagtcgcta tagtaccaat
       61 gaagttaaat aacaaaagat tgccacaaaa aaacacgaaa gcttttacca atggaaaacc
      121 attgtgttat tatatacttt caacactttt aaaagtaaaa cagattgata aagtgtatgt
      181 ctattgtagt aacccagata ttaaagaatt tataccagaa ggtgtaacat ttttaaagag
      241 atctagttcc ttagatcagg atacaacgag tattacacag gtattagccg gtttcacaaa
      301 agaagtagag gctgatgtat atgtaatgac acacacaaca gcaccattta ttagcgaaaa
      361 atctattgaa aaaggactag atgccgtgct gacaggagaa tatgattctg catttgctgc
      421 aaaaaaattg caagatttct tatggaaaga cggtgaacca tttaattatg aattggacaa
      481 tattcctaga acacaggatt tgccgccttt atacgaagaa actagtggat tttatattta
      541 taatcgcaat gtgatgaccg agttgcatag aagaattggg aaaaaacctt gcatagttga
      601 agtaggtgag attgagtcag ttgatattga tgaagccgag gattttatga ttgctgatgc
      661 gatttataat catcttattt tacaaggtag ttaagacagg gggaaggtta ggatgaacaa
      721 tgtacagatt ttagattgca cacttagaga tgggggctat tgtaacaatt gggaatttgg
      781 atttgataat acaaaaaaaa ttatttctgg acttatagaa tcaggaattg agattataga
      841 atgtgggttt ctcacaaata aaacagtata caataaaaat gttacaaaat atacgacctt
      901 agaagaatta gcacagataa ttcctgaaaa aagagaagga aaattatttg tagcaatgat
      961 gaactatggc gaatataata tagaggatct tccagattat gatggttcat ctattgatgg
     1021 aattaggatt gcgtttcata aaaaaaattg caatgaagcg ttgaaattat gtcgtgagat
     1081 aaagaaaaaa ggctacttag tttttgttca agctatggta tctctcaatt atacagatga
     1141 agagttttta gcagtaataa aaaatgtgaa tcagtttaaa ccatacgctt tttatattgt
     1201 tgatagtttt ggaatgatga aaggaaaaga tttaactcgt cttttttaca tgattgagca
     1261 taatctagaa aaagatatac agataggttt tcactcacat aataacatgc agcttgcata
     1321 ttctaatgca caaaaactta taaccgtaca aacaaatcga agcctgatta ttgattcttc
     1381 ggtgtacgga atgggaagag gagcaggtaa tcttaacaca gaactatttg tagaatattt
     1441 gaatgataat gcaggaaaaa aatatcaatt aaaaccatta ttatatataa ttgatgaaat
     1501 actaaatagt ttctatcaga agagttattg gggatattca ttaccaaact atatttcagc
     1561 agttcataat gctcatccta attatgcaag ctatctgtca tataagaata ctttaacaat
     1621 tgaagcaatg aatgaaatat tcgaaatgat ggatgaagaa aaaaaggtat cttatgataa
     1681 acaatatata gaagatttat actacaaata tatggaaact gggaaaatac aggaggaata
     1741 tatggaagaa ctgagaaaat ttctctcaga caaaaaaatt ctactgattg caccaggaaa
     1801 aagttctgta gatgaggtag aaagaataaa agagtttgtt accaaagaag aagtcgtaat
     1861 aattagtgta aattttgaat attctcatat agatatagat tacgtatttt taagtaattt
     1921 aagacgtttt catgaattac cgaaagaaag cagaactaaa tgtattgtta catcaaatat
     1981 acaagcatcg gatgtatatt tacggacgag ttataaaaag ttgttatctg aaagggaagt
     2041 tgtaagagac aatgcaggat taatggcaat tagattttta gccgatatga atgtggatga
     2101 aatattctta gctggatttg atggatattc acataatgaa gatgagaatt atggtgagca
     2161 atccatggaa attataacaa aatttgcatt attagatgca atgaacgtgc aaatgagaga
     2221 tgaattgtca gaattagcta aaaaagtaaa ggtgacattt ttaacggatc cacgttgggt
     2281 tcgaattgat aagtagtagt aataatgttt ttaaaatttt gataaagaga tagtgagaga
     2341 ataaaaatgg gtaaagtctt aaataaaaaa gaagaaaaat atttaaggga cgatggggcg
     2401 agatgaaact ctaattttga acttttaaga atcatttcta tattgtgtat tgttgcttac
     2461 catgctaatt tgcgtttaaa ttataatgca gacggaatta tagtacatgg ttcattaact
     2521 gctaaccaga ttttcttgaa ttatattggt atttggggtc agctaggagt taattgtttt
     2581 atatttattt cggcatattt tttaagtgag aaaagaaaca ttcactttga aaatgttttg
     2641 ggaattgtat tagaatgtat tttttatagt gtattagttt atggaattct ttgtgcatgt
     2701 ggaatagaaa cattttcatt gaacagcttt gtaaaagaaa tattatcatt tgctctaggt
     2761 agatattggt ttattacatc ctatttagca ttgtatattc taagtccgta tttaaatatc
     2821 atgcttaaca atctggaaaa acttcagttg caaaaaataa gtatgttatt gtttataggt
     2881 gtatgctgtt atcgtatgtt atttaaaaca tctttgtttg gggattgtgc tgttttaatt
     2941 gcactatatg tcatatgtta tacactgaaa aaaacgataa taatattttt gagagacaca
     3001 gaatcatagg aacaattgta tgtataacag ttataccact tctcatatcc ataggaatac
     3061 ttttcaaatt tataccaaat gcaatggtta accagatggt tcatattggt tctccattga
     3121 tggttatagc aagtatattt ttattttatg tatttaaaaa tttttcattt tacagtaagt
     3181 atataaataa acttgcgaga accagtttag gggtatatat tttacatgaa aatcattatt
     3241 tcattgatgg tatgaagtct agaggcttaa tatgggatgg gatatttgga ttaggcaaaa
     3301 ggtttttaga tagggatttt tggatttggt ttataattgc agttgtagtt gtgtgcataa
     3361 tttgcatggc tatcgatgtt gtaagagaaa aatttgaaag acagattttt aaaattaaga
     3421 gtgtaaagaa cctgtgtaaa cttatgaatc atgttataaa tatataccca taattaataa
     3481 atgaggagaa aagaagtgaa agttatatca ttcggagaat tattattgag acttgcagca
     3541 cccggttatt caaagttatt tcaaagagat agtttggaga ctacattctg cggaggggaa
     3601 gctaatgtag cggtatcctt agcaatattt ggatatcaat cagaatttat aacaaagctt
     3661 ccggattcag atgttggata tgcggcagct aatgctttgc gttattttgg agtaaatgtt
     3721 gataatatta tatttggaga gggacgcatg ggactgtatt atttagaaaa aggagcagct
     3781 cagagaccat ctaaagtaat ttatgacaga aaattttctg caatttcgtt agctgatagt
     3841 gatgaattta agtggaagga gatcctaaac gatgcaaatt ggtttcattg gacagggata
     3901 aatccagcat tgtctgagaa tctctttgag acgtccttag atgcatgtca ggctgccaaa
     3961 aaaagaggta taacaatttc ttgtgattta aattatcgat ccaagctttg gacatcagag
     4021 gaagcaaaag aaaagatgga acagatacta ccattggttg atatttgtat ttgtaatgaa
     4081 gaagatgcag aaaaagtgct aggaattaaa gctgagcata caaatattaa tagtggagaa
     4141 ttaaataaag atggctatgt gcaggtggct agggagattc acaagaagtt tggatgtagg
     4201 tatgtagcta caactctgcg tagaagttat tcggcaagta gaaatggttg gagtgcaatg
     4261 ttatacgaag cagaaaaaga taaagcgtat ttttccacag aatacaacat acaaattgtt
     4321 gatcgcgttg gtggtggaga cagcttttct gctggtataa tatatgcgtt atgtgaaaat
     4381 atgtccggac aaaaggctat tgaatttgcg actgcatcta gttgtttaaa acatactata
     4441 gaaggtgatt ttaatcgaac tacaattgag gatgtatata atttaatgaa tagcggtgga
     4501 aatggtcgcg tgttgcgtta gtcaacatat aaagataaat cacagtaatt tttatggtga
     4561 ttatctgtct tagtagtgat tagaataacg tgaaaataaa ttgttgagcg tttatactgt
     4621 tttgaaaaat aaatttaaca tattgaaaaa tacaaatata tacgtatatt ataaagcgcc
     4681 gattgtaaaa ttaagctaga caactaaaac agtcatttgt gctacactca aaatagttcc
     4741 gaccaaagaa ttataaggag tgagtgcaaa taacctatac ctatcttaca acggatgaac
     4801 ttgtaataat agagtcttat ttgaaaatta accaatctgt tgctaaaact gcgcattgct
     4861 tgaatcgttc aagtcaaacg atccataaag tatatctgtt cttcaagcaa agaaaatcag
     4921 ccttagaata
//
LOCUS       DASJPC010000143         4788 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJPC010000143 DASJPC010000000
VERSION     DASJPC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.148"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            564..989
                     /locus_tag="U4A13_002663"
     CDS             564..989
                     /locus_tag="U4A13_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780915.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="U4A13_002664"
     CDS             1019..1183
                     /locus_tag="U4A13_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780916.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="U4A13_002665"
     CDS             2141..3118
                     /locus_tag="U4A13_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1780917.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="U4A13_002666"
     CDS             3128..3625
                     /locus_tag="U4A13_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1780918.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="U4A13_002667"
     CDS             4001..4282
                     /locus_tag="U4A13_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780919.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="U4A13_002668"
     CDS             4284..4700
                     /locus_tag="U4A13_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780920.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DASJPC010000144         4720 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJPC010000144 DASJPC010000000
VERSION     DASJPC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.149"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1097)
                     /locus_tag="U4A13_002669"
     CDS             complement(<1..1097)
                     /locus_tag="U4A13_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1780921.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="U4A13_002670"
     CDS             complement(1227..2603)
                     /locus_tag="U4A13_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1780922.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="U4A13_002671"
     CDS             complement(2603..3259)
                     /locus_tag="U4A13_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN1780923.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="U4A13_002672"
     CDS             complement(3269..4546)
                     /locus_tag="U4A13_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN1780924.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="U4A13_002673"
     CDS             complement(4565..>4720)
                     /locus_tag="U4A13_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN1780925.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DASJPC010000145         4714 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJPC010000145 DASJPC010000000
VERSION     DASJPC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.150"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            108..359
                     /locus_tag="U4A13_002674"
                     /pseudo
     CDS             108..359
                     /locus_tag="U4A13_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            452..1246
                     /locus_tag="U4A13_002675"
                     /note="aph(3')-IIIa"
     CDS             452..1246
                     /locus_tag="U4A13_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1780926.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1656..1739
                     /locus_tag="U4A13_002676"
                     /pseudo
     CDS             1656..1739
                     /locus_tag="U4A13_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1788..1871
                     /locus_tag="U4A13_002677"
     CDS             1788..1871
                     /locus_tag="U4A13_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEN1780927.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1996..2733
                     /gene="erm(B)"
                     /locus_tag="U4A13_002678"
     CDS             1996..2733
                     /gene="erm(B)"
                     /locus_tag="U4A13_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1780928.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2996..3859)
                     /locus_tag="U4A13_002679"
     CDS             complement(2996..3859)
                     /locus_tag="U4A13_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEN1780929.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3861..4133)
                     /locus_tag="U4A13_002680"
     CDS             complement(3861..4133)
                     /locus_tag="U4A13_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1780930.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4150..4359)
                     /locus_tag="U4A13_002681"
     CDS             complement(4150..4359)
                     /locus_tag="U4A13_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN1780931.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4457..>4714)
                     /locus_tag="U4A13_002682"
     CDS             complement(4457..>4714)
                     /locus_tag="U4A13_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEN1780932.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1447 a          796 c          865 g         1606 t
ORIGIN      
        1 atttcttggg agttttgaga ctactatatg cgtttttttg ttgatttacc aacatgttta
       61 gacattttcc tctaggaaaa aattaactag caccacgggt tatcttttat atagaagata
      121 tcgccgtatg taaggatttc agggggcaag gcataggcag cgcgcttatc aatatatcta
      181 tagaatgggc aaagcataaa aacttgcatg gactaatgct tgaaacccag gacaataacc
      241 ttatagcttg taaattctat cataattgtg gtttcaaaat cggctccgtc gatactatgt
      301 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
      361 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
      421 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
      481 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
      541 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
      601 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
      661 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
      721 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
      781 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
      841 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
      901 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
      961 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
     1021 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
     1081 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
     1141 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
     1201 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagatttaga
     1261 tgtctaaaaa gctttaacta caagcttttt agacatctaa tcttttctga agtacatccg
     1321 caactgtcca tactctgatg ttttatatct tttctaaaag ttcgctagat aggggtcccg
     1381 agcgcctacg aggaatttgt atcgataaga aatagattta aaaatttcgc tgttattttg
     1441 tacatttaac ttgacggtga catctctcta ttgtgagtta ttagtggtac agttttcaac
     1501 cgttttaatt ataaaaaagt ggtgcatttt taaattggca caaacaggta acggttattg
     1561 caggtgtatt tcttatctat gggtttaaca tggattttat cattaaaatc atgagtattg
     1621 tccgagagtg attggtcttg cgtatggtta accctaaagt tatggaaata agacttagaa
     1681 gcaaacttaa gagtgtgttg atagtgcatt atcttaaaat tttgtataat aggaattgaa
     1741 gttaaattag atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc
     1801 aaatgcgtta tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg
     1861 cagataaata aatacgttag attaattcct accagtgact aatcttatga ctttttaaac
     1921 agataactaa aattacaaac aaatcgttta acttctgtat ttgtttatag atgtaatcac
     1981 ttcaggagtg ataacatgaa caaaaatata aaatattctc aaaacttttt aacgagtgaa
     2041 aaagtactca accaaataat aaaacaattg aatttaaaag aaaccgatac cgtttacgaa
     2101 attggaacag gtaaagggca tttaacgacg aaactggcta aaataagtaa acaggtaacg
     2161 tctattgaat tagacagtca tctattcaac ttatcgtcag aaaaattaaa actgaatact
     2221 cgtgtcactt taattcacca agatattcta cagtttcaat tccctaacaa acagaggtat
     2281 aaaattgttg ggaatattcc ttaccattta agcacacaaa ttattaaaaa agtggttttt
     2341 gaaagccgtg cgtctgacat ctatctgatt gttgaagaag gattctacaa gcgtaccttg
     2401 gatattcacc gaacactagg gttgctcttg cacactcaag tctcgattca gcaattgctt
     2461 aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt
     2521 acccgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca
     2581 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg
     2641 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt
     2701 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg
     2761 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatagggc acctctaata
     2821 actaccaatt aattcacctc aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta
     2881 tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt
     2941 ataaaaaaag agtagttacc gttttttggt aactactctt ttcctttttt aaggtttaaa
     3001 tacctggaag tttaggtgtt ttgggtattg gtggtgtagg tggctgcaag ctttccaatt
     3061 tttgttgaat tgctttaaat tcaggtgtct cttggtgttt atttagaacc attttttgct
     3121 ctattcgttc taaagtcggt tgaatttctt tccctgatac tttacgattc aattcttttt
     3181 ctaaggtttc ttttggacta atagaaggtg tttccaaact tgaatagagt tttactcctt
     3241 ctctgttata tagccttata tcgctaaata agcccgtttt atgaagggtt tctaaattgg
     3301 tcggtaagtt tttgacaaca atatcatgcg cttgttttgg tgttgccctg gctgtcattg
     3361 gatcatctgc atacatggtt tcatatcgtt caattgttcc taaatatgag ttgattttag
     3421 gtactgccat gacatacatt tttgtttcat aacctttggc ttgaagcatt gttgcggttt
     3481 gaataggaac gtctgttgtt cgtcctgtac cttcgatcac caaattatac ccttgatcgc
     3541 tcaaacggct tatgatcgct tctgtcatgc gattagaata aggggtaacg tgttttacta
     3601 cgtctttttc ataaagtttc actagttcat caaaattagg gtgctgttgt ttaaaggtat
     3661 cattatcaat gacaataaca ttcccttgtg tttcttcaaa aattgctgat cgcaaactgg
     3721 ttttccctga ccctggttgc ccaccaagta aaaaagcggt tggcgattca accgcttttt
     3781 ttccttgaat caattcttct aaattatcat ttaagcgatt ctcaaattgt ttgtcagtaa
     3841 aattgactat atttgccata ttaagccact ttctctttat tcaaaacttg ttttgagtaa
     3901 cgattcattg accgccaata ctcatgaacg gcttgtaatt cttctaaaga ataatcaaaa
     3961 agatttacac gttttgcaag ctttaattgg tttaataaat tgacttccaa taattgagag
     4021 tcatttttat ttaattcatg gttcaaaaca tagtttaata ctcgattata aacgcttgcc
     4081 gataattcgt taatgatctc tatttcaaat gttttttcat acgtaactgc catttcattt
     4141 tcactccttt taaagtttgt caggtaaata tttccgtata tattcttcta aggcttgatc
     4201 cataacttct tttacgtttc cgccattctt tgctgtttcg atttttatga tatggtgcaa
     4261 gtcagcacga acacgaaccg tcttatctcc cattatatct ttttttgcac tgattggtgt
     4321 atcatttcgt tttgcttttt gtgcgcctaa atttcccaca atcactcact tctttctatt
     4381 tcttcttact cttattttat catcaacaat cacaaatcac aagtgatttg tgattaatca
     4441 cttgtgattt gtgattctat tctttttcgt tttctaattg aataattcgc tcaagcattt
     4501 caaaaaatac gttcttatac atggataaaa cttttttgtc atagccttta tgctctgtaa
     4561 tgccgttttt agaccaggta cttactttat tacttcgctt gataatattt tggaaaacca
     4621 agttatcttc tttatgttgc ttatacagtt cttccaggtt tgattttatc gttgcgctgt
     4681 ccgtatcaac taaataagga acaaaaccga tcat
//
LOCUS       DASJPC010000146         4646 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJPC010000146 DASJPC010000000
VERSION     DASJPC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.151"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(133..717)
                     /locus_tag="U4A13_002683"
     CDS             complement(133..717)
                     /locus_tag="U4A13_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1780933.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="U4A13_002684"
     CDS             complement(928..1131)
                     /locus_tag="U4A13_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780934.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="U4A13_002685"
     CDS             complement(1141..1563)
                     /locus_tag="U4A13_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780935.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="U4A13_002686"
     CDS             1801..2415
                     /locus_tag="U4A13_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1780936.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="U4A13_002687"
     CDS             complement(2580..3404)
                     /locus_tag="U4A13_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780937.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="U4A13_002688"
     CDS             complement(3428..3583)
                     /locus_tag="U4A13_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780938.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="U4A13_002689"
     CDS             complement(4042..4299)
                     /locus_tag="U4A13_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1780939.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="U4A13_002690"
     CDS             complement(4292..4561)
                     /locus_tag="U4A13_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1780940.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DASJPC010000147         4634 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJPC010000147 DASJPC010000000
VERSION     DASJPC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.152"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..518
                     /locus_tag="U4A13_002691"
     CDS             174..518
                     /locus_tag="U4A13_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN1780941.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="U4A13_002692"
     CDS             519..938
                     /locus_tag="U4A13_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN1780942.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="U4A13_002693"
     CDS             complement(1030..1380)
                     /locus_tag="U4A13_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780943.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="U4A13_002694"
     CDS             complement(1346..2566)
                     /locus_tag="U4A13_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN1780944.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="U4A13_002695"
     CDS             2932..3498
                     /locus_tag="U4A13_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN1780945.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="U4A13_002696"
     tRNA            3643..3730
                     /locus_tag="U4A13_002696"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="U4A13_002697"
     CDS             4064..>4634
                     /locus_tag="U4A13_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN1780946.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DASJPC010000148         4610 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJPC010000148 DASJPC010000000
VERSION     DASJPC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.153"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(85..309)
                     /locus_tag="U4A13_002698"
     CDS             complement(85..309)
                     /locus_tag="U4A13_002698"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1780947.1"
                     /translation="MKKKISTIFFTNKQRYGATKIHQVLLKEGISVSLKHVQKLMKQL
                     NLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="U4A13_002699"
     CDS             complement(450..704)
                     /locus_tag="U4A13_002699"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1780948.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="U4A13_002700"
     CDS             complement(897..1172)
                     /locus_tag="U4A13_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN1780949.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="U4A13_002701"
     CDS             complement(1144..2097)
                     /locus_tag="U4A13_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1780950.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="U4A13_002702"
     CDS             2709..4202
                     /locus_tag="U4A13_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN1780951.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="U4A13_002703"
     CDS             4337..>4610
                     /locus_tag="U4A13_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN1780952.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DASJPC010000149         4588 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.154, whole genome shotgun sequence.
ACCESSION   DASJPC010000149 DASJPC010000000
VERSION     DASJPC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.154"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(67..1041)
                     /gene="lacD"
                     /locus_tag="U4A13_002704"
     CDS             complement(67..1041)
                     /gene="lacD"
                     /locus_tag="U4A13_002704"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN1780953.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(1038..1496)
                     /locus_tag="U4A13_002705"
     CDS             complement(1038..1496)
                     /locus_tag="U4A13_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN1780954.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1493..2956)
                     /locus_tag="U4A13_002706"
     CDS             complement(1493..2956)
                     /locus_tag="U4A13_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN1780955.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2938..3870)
                     /gene="pfkB"
                     /locus_tag="U4A13_002707"
     CDS             complement(2938..3870)
                     /gene="pfkB"
                     /locus_tag="U4A13_002707"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN1780956.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            4012..>4588
                     /locus_tag="U4A13_002708"
     CDS             4012..>4588
                     /locus_tag="U4A13_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN1780957.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         1375 a         1028 c          728 g         1457 t
ORIGIN      
        1 atttgacaaa cattttttta aataactaat tattttttga atctgtcact cagacaatcc
       61 tttctcttac ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc
      121 tttaatattt tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt
      181 aacaaaaggt gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt
      241 cgaacctgat tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc
      301 acttaaaaaa ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc
      361 ctctgcttga ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg
      421 tacttctact ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc
      481 aatcacttta tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc
      541 atcgtaagaa aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat
      601 ccgttctata actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta
      661 caataaaaat ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt
      721 cagtaaatct ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag
      781 caacaaacca cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag
      841 taaaatagcg gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat
      901 atcttctgat gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc
      961 gatagctagc gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt
     1021 ttctatactc attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt
     1081 tctatttttg attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt
     1141 gcaacgagag acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac
     1201 gcaaaaataa aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt
     1261 gtgacgataa tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa
     1321 cctgacatta ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct
     1381 ttttcgtcat gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct
     1441 tcttgattat ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata
     1501 aacctcctgc tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct
     1561 tgtctttatc agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg
     1621 aaccaataag aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa
     1681 aaccgccatg tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg
     1741 atgagcctaa catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag
     1801 ctccttccgt aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat
     1861 tagtatcaaa atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa
     1921 tgcaagctgc tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc
     1981 ctgttacata tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa
     2041 caatcacgcc aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc
     2101 cttcatttat agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta
     2161 caccaataac tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta
     2221 tcagtagctt ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc
     2281 ctaaaaatcc agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc
     2341 ctggacgttt cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca
     2401 ttgctgcatc gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg
     2461 gatcagaaga atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta
     2521 ccacgaaagg caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa
     2581 aattagcacc acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga
     2641 cagtaccttt tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac
     2701 tgacagaaac atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg
     2761 cggcaataat gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc
     2821 caatttgtcc atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta
     2881 atgcctcttg cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta
     2941 gttgatattt tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc
     3001 ctccacatcc aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat
     3061 gcttttacgt aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc
     3121 taacatggca tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg
     3181 aatcaccctg tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt
     3241 ttctgcgcct gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat
     3301 ttttcgtccg aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata
     3361 gggtaaacaa tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat
     3421 ttctattaaa acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc
     3481 ccctgcttgt aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc
     3541 aggtccttga ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt
     3601 gatacgcgtc ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata
     3661 gtcttctata tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc
     3721 tagcatcttt aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc
     3781 ttttgtgcgg tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc
     3841 caaattcatc gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa
     3901 atacgcgaaa tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta
     3961 taaactcgtc gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac
     4021 aaattaagtg aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc
     4081 cctaactatt ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg
     4141 cggacaatga aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa
     4201 ggagaaaaca agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga
     4261 gccattctaa aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac
     4321 attgaagatg caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga
     4381 ttttcagaaa tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt
     4441 cagatgcata ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac
     4501 agcgttattt ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt
     4561 tatgtcttta tttttcaatt ttttaaat
//
LOCUS       DASJPC010000150         4473 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJPC010000150 DASJPC010000000
VERSION     DASJPC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.155"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            321..1052
                     /locus_tag="U4A13_002709"
     CDS             321..1052
                     /locus_tag="U4A13_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN1780958.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="U4A13_002710"
     CDS             1197..3224
                     /locus_tag="U4A13_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN1780959.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="U4A13_002711"
     CDS             3332..4429
                     /locus_tag="U4A13_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN1780960.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DASJPC010000151         4470 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJPC010000151 DASJPC010000000
VERSION     DASJPC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.156"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            138..1475
                     /locus_tag="U4A13_002712"
     CDS             138..1475
                     /locus_tag="U4A13_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN1780961.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="U4A13_002713"
     CDS             1523..3652
                     /locus_tag="U4A13_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN1780962.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="U4A13_002714"
     CDS             3627..4316
                     /locus_tag="U4A13_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780963.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
BASE COUNT         1388 a          795 c         1007 g         1280 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca
//
LOCUS       DASJPC010000152         4434 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJPC010000152 DASJPC010000000
VERSION     DASJPC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.157"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="U4A13_002715"
     CDS             complement(543..2048)
                     /locus_tag="U4A13_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1780964.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U4A13_002716"
     CDS             complement(2045..2731)
                     /locus_tag="U4A13_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1780965.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4A13_002717"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4A13_002717"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN1780966.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DASJPC010000153         4324 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJPC010000153 DASJPC010000000
VERSION     DASJPC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.158"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..281)
                     /locus_tag="U4A13_002718"
     CDS             complement(<1..281)
                     /locus_tag="U4A13_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1780967.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="U4A13_002719"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="U4A13_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="U4A13_002720"
     CDS             complement(2359..2721)
                     /locus_tag="U4A13_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780968.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="U4A13_002721"
     CDS             complement(2725..3063)
                     /locus_tag="U4A13_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780969.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="U4A13_002722"
     CDS             complement(3042..3461)
                     /locus_tag="U4A13_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780970.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="U4A13_002723"
     CDS             complement(3454..3687)
                     /locus_tag="U4A13_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780971.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="U4A13_002724"
     CDS             complement(3684..>4324)
                     /locus_tag="U4A13_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN1780972.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DASJPC010000154         4219 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.159, whole genome shotgun sequence.
ACCESSION   DASJPC010000154 DASJPC010000000
VERSION     DASJPC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.159"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(37..1257)
                     /locus_tag="U4A13_002725"
     CDS             complement(37..1257)
                     /locus_tag="U4A13_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEN1780973.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="U4A13_002726"
     CDS             complement(1277..1888)
                     /locus_tag="U4A13_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN1780974.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="U4A13_002727"
     CDS             complement(1931..2863)
                     /locus_tag="U4A13_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN1780975.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="U4A13_002728"
     CDS             complement(3284..4144)
                     /locus_tag="U4A13_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1780976.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1198 a          853 c          690 g         1478 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgctt
//
LOCUS       DASJPC010000155         4101 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJPC010000155 DASJPC010000000
VERSION     DASJPC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.160"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..555
                     /locus_tag="U4A13_002729"
     CDS             <1..555
                     /locus_tag="U4A13_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1780977.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRN
                     ISHKVRVDDRKEVCDDFKMVYQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEGELEQMLSQLIEN"
     gene            689..2878
                     /locus_tag="U4A13_002730"
     CDS             689..2878
                     /locus_tag="U4A13_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN1780978.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2875..>4101
                     /locus_tag="U4A13_002731"
     CDS             2875..>4101
                     /locus_tag="U4A13_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN1780979.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1509 a          589 c          710 g         1293 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttcccgta atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg tggtactttt
      241 gcggaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataaaca aatcaagaaa tacagccacc gcaaggaaca gttccaaaat
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctaagccaa
      541 ctgattgaga attagaaaca atctgcagta gggaagaatc atttacacaa aattattgac
      601 gctcccttat aatgagaact tttactaaca taatttatga taaacttact atataatcat
      661 attaattaaa tagttaggag atctgcttat gtttatatct actcttcaaa ttcgaaattt
      721 tagaaattat gaatccgaaa tctttcgatt cgataatgag acaaatacta ttataggtga
      781 gaatgattca gggaaaacca atgctcttac tgcattgaga atcttattag atgatagtta
      841 ttactatagt agtaaaacac taaaagaatc agattttttt catggaattc aaaatgggtg
      901 gcaagggcat tggattatta tctctgcgac atttgaagga atcagcgaag aagaatttga
      961 caatgagatt tgtgcttcca tttctttaaa ttctgaaagt caaacaatct tagaagaatt
     1021 gatttcaaat gcagataaag gtgtaggtag tatttctcta tttattagac ctaacaaagc
     1081 tattagaaag caattatttg atatttccac tgaattagac tctcatcaat ttaacgaatt
     1141 tagaaattct attcgtctat ccgactatga attttactac acatctaaat caaatctaaa
     1201 tttctgtatc aatgaaaatt atgataaatt ggtaggaaga ctcaatgaca atatagctag
     1261 taatcctgaa gaagacgatg aagctctgtt gggctcaaga attgacatgt cagatatttt
     1321 caagcatatt tcggttgttt atgttgacgc tcttcgagat gttcttagag aaatgaaaaa
     1381 caatcgaaac ccagtcaaaa gaatcatgga aacaattgaa tcaaaaatat catcagataa
     1441 tgttgagtca ttaaaaacaa ttatacagca attaaatgag actattacca gtgttcctga
     1501 aattaggaaa attggcgaaa atattaatcg gcaactaaat agcataattg gttcagtgta
     1561 ttctcctaat cttcttcttt cctcaactat gtcagatgat atgggttctt tagcaaaatt
     1621 tctttcaatg aagccagaac aaaatatcga cttagactta ctagggttag gacatctaaa
     1681 tatgatttat ttagctttga aaatagtcga atttgaagcg tgtcgttcaa gagaactact
     1741 taatattatg ttgattgaag aaccagaagc tcatattcat catcatattc aaaaaactct
     1801 gttcgaagga ttaaatcttc aaaaaaacta tactcagatt ctcatgacca cacattctgt
     1861 acatttagct gaagtgtctg aaatatctcg gatgaatgta ttaaaatcgt ttaatggcaa
     1921 atctatcgtt atgaatccag caaataatct gaactcattt gctaaagaaa agttaaaaaa
     1981 gaacaatcta aatctaataa acgcagtaga gagatattta gatgttaaaa gaaatggatt
     2041 gcttttttct aaaggtgtta tcttagttga aggagatgct gaagaaatac ttattcctca
     2101 aatagtaaaa aaattatttg gtataaactt agacgaactt ggaataggtt tgattaatgt
     2161 tggtagtact tcgtttgaat atatcgctag cttgtttgac gatgatagaa tacagagaaa
     2221 gtgtgctatt gtaactgatt tagatgaaca ggcaatccca tcagaacatc gattgtataa
     2281 aagcactgca caagagaaag gtcaagcaag aaaagaaaaa ttatcctccc tttttaatga
     2341 aaacaattgg gttaattgtt tttatgctaa tacaacattt gaaattgagt ttttagatgt
     2401 gaataaagaa aatattctaa cttatgtcag tccaattata gatgacgcat tcaaagataa
     2461 aaaaacaata actgcttata aagaagaaat acaaagtgaa caatatttag aaagaaattt
     2521 agcgatgtta aaactagcca atcatgttgg taaggggtgg tttgcaattg agttaggtga
     2581 aaaaattgac tttttaatca aaattcctaa gtatattctt aatgctattg cttttgcatg
     2641 tcaggaaata gtagatctct caatttataa aaaaattcta ctccatacaa tcgacttgta
     2701 cgaggaaact acagaattgt cagctttatc agctagttta aaaacaagtg atacagctga
     2761 tttgaatata gcaataattt ctttattaga gtactttggt aaagatgagg atgttgtgta
     2821 tctaatcaat cagatagaaa attacacaaa aatactttat gctgaggttt caaaatgaca
     2881 aatatcttcg aaaaaatcgt aaatgattta aatttagaac aaaaagatgt tgtggaaagt
     2941 gaagataacc tttatgtaaa tgcatgtcct gggagtggga aaacaagggt acttacaagg
     3001 aaaattgctt accaagcaat taaacacgat ggatcactaa aaagaataat tgctatcaca
     3061 tacactaata gagctgctga agaaattaag gaacgcctaa gtttacttag tatagatgat
     3121 gatattaata tttgggtggg gacaatacat cagttttgtc ttgaatttat tatttatcct
     3181 tttaaaatga atctctcaag agtatcaaaa ggatttacaa ttattgatga ttatactcaa
     3241 agagcttacg tcagccaaat taataatatg ctgaatttaa atctcaaaac gtatgaactg
     3301 aacaaaattg atttgtcact ttctcatgat tttaaaatta atgaagttca gtatactcaa
     3361 gtttctgaaa tgtatcacac tatgttggaa aaaaataaag aaattgattt tgatatgatt
     3421 ttactagtag cgtataaaat tttaactgta aacagtaacg ttgcacgtaa tattgctagt
     3481 aatataaggg cgatttatgt ggatgaattt caagatacta gagaattaca atacaatgta
     3541 atagctaaat tacttcaaaa caaccctcaa attcagtcaa tgtttgttgg agatattgac
     3601 caagcaattt atggatcatt agatggtata gctaaatcag ttgaagaact agaaatgcta
     3661 actagtcaca cttttcaatc aaagacgcta catggctgtt acaggtctaa tcagagatta
     3721 gtagacttct actctaattt tcagagttgt ccttacgaga ttgaaagcag agggaataat
     3781 aaaaacgacc gtggttttat aagttatgat tgcaaagtta ctaaggagga tttacccaac
     3841 ataataaaaa atataataaa ggaaaagatt gaaagcggca ttcatgaaaa agatatttgt
     3901 gtacttgctc ctcaacacta tcctctgttt tcactgggtg agtatttaaa gaaagaatta
     3961 ccgtattgca attttgatgc tataaatata tcaccaatta aggtaaataa tcatagttta
     4021 tttttcaagt tagcaatttt gtactttact gaatcaggtg aaaaggttcg aagaagaaag
     4081 ctaattgcaa acgatatttt a
//
LOCUS       DASJPC010000156         4036 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJPC010000156 DASJPC010000000
VERSION     DASJPC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.161"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(242..391)
                     /locus_tag="U4A13_002732"
     CDS             complement(242..391)
                     /locus_tag="U4A13_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780980.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="U4A13_002733"
     CDS             853..1065
                     /locus_tag="U4A13_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780981.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="U4A13_002734"
     CDS             complement(1343..3142)
                     /locus_tag="U4A13_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN1780982.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="U4A13_002735"
     CDS             complement(3266..3862)
                     /locus_tag="U4A13_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN1780983.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DASJPC010000157         3959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJPC010000157 DASJPC010000000
VERSION     DASJPC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.163"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(609..1166)
                     /locus_tag="U4A13_002736"
     CDS             complement(609..1166)
                     /locus_tag="U4A13_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780984.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1345..1956)
                     /locus_tag="U4A13_002737"
     CDS             complement(1345..1956)
                     /locus_tag="U4A13_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780985.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2069..2326)
                     /locus_tag="U4A13_002738"
     CDS             complement(2069..2326)
                     /locus_tag="U4A13_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780986.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2923..3201)
                     /locus_tag="U4A13_002739"
     CDS             complement(2923..3201)
                     /locus_tag="U4A13_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780987.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3235..3486)
                     /locus_tag="U4A13_002740"
     CDS             complement(3235..3486)
                     /locus_tag="U4A13_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780988.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1200 a          810 c          526 g         1423 t
ORIGIN      
        1 tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa tggtgtccaa
       61 aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat tatctccacc
      121 aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat tttcatacaa
      181 aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac tagtatccca
      241 atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct tttatgtatg
      301 ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa caagttgttt
      361 ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca
      421 tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc tcagctaata
      481 gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat ttcaatggtt
      541 ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa tggattagta
      601 gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact aataagacgg
      661 attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc tatttcaggt
      721 gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt aggattaggc
      781 tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac ataatatatt
      841 cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc acttgctaaa
      901 tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat
      961 aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata ttctaatcta
     1021 aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa tattattcta
     1081 cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat gattaatatt
     1141 ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc tcacatatta
     1201 gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt cgctatgaat
     1261 tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat gatttggctt
     1321 gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg gttcagatat
     1381 ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt
     1441 aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc ttttaaaacc
     1501 ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta cattttcccc
     1561 catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc caaaaggtag
     1621 atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca tagaacctgc
     1681 gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt taatctgtcc
     1741 atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg gttcgggtat
     1801 tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca aaaaaactcc
     1861 tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc tcattgatcc
     1921 attgtattcc attttattcc ccctattact attcatatcc gcctatctat atcgatcttc
     1981 caatgcagag ataattgctt ccataccttg attttttact gagttaccta tgcgagatat
     2041 cattctccac taaggtgcat catattgtct atctaacatg attaattctc caataggtgt
     2101 atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct accatgtttg
     2161 ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct ggattaaata
     2221 taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct tttgaatgtc
     2281 cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt taccctgacc
     2341 attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc tcctaacttc
     2401 actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc atttaattcg
     2461 gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt atcaataaac
     2521 tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca taatatcatt
     2581 gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa
     2641 gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa ctatttgtaa
     2701 tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga ttcccaagca
     2761 aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc ttatataaat
     2821 tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg cttatatcac
     2881 aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt tttcaaattg
     2941 tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt taacattatc
     3001 atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta taattaacct
     3061 tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat ctctcctaat
     3121 ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat acatactgcc
     3181 tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc gttcttactt
     3241 ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca acaataagaa
     3301 tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat aagtaggatt
     3361 agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag cacaaacacc
     3421 aaagataaca actccaaaca ttgaattttt atacaatgac catttattta taggcttttc
     3481 tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag ttcataagcc
     3541 ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc ttgttttaag
     3601 cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg ccgaaaagtc
     3661 tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac tatttgtgaa
     3721 acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag tagctcttaa
     3781 ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat cccctctaaa
     3841 aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa tagagcatta
     3901 ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca attatttgt
//
LOCUS       DASJPC010000158         3937 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJPC010000158 DASJPC010000000
VERSION     DASJPC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.165"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            256..744
                     /locus_tag="U4A13_002741"
     CDS             256..744
                     /locus_tag="U4A13_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN1780989.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            2078..2380
                     /locus_tag="U4A13_002742"
     CDS             2078..2380
                     /locus_tag="U4A13_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN1780990.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            2373..3401
                     /locus_tag="U4A13_002743"
     CDS             2373..3401
                     /locus_tag="U4A13_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEN1780991.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            3457..3675
                     /locus_tag="U4A13_002744"
     CDS             3457..3675
                     /locus_tag="U4A13_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1780992.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT         1513 a          415 c          675 g         1334 t
ORIGIN      
        1 ggaagcggtt acttattctt ttgaatctaa gataaaaatt caaaagaata acataaccca
       61 aatatgatta tacttgagat tctaaatatt tatcgaaaag gaatttgttt taaggaaaaa
      121 tgattctatg gacggttatt gatttatatt taccaaatgg ctaataaaat agtatttctt
      181 atattgtata gtttttgttt ttatgtgaaa acatgagcaa tagaattgta ctagtaggat
      241 aaggatggat ggaaaatgaa aaaaactatt tttgttttag cgatttttat atgttttggg
      301 ctacttttgg taggctactt gttgtttaat tctaatgaaa tgagaactat ttctacgaat
      361 gacttgttaa aaaaaagtga attaaaggat aaaaaaaaag aatctcttgc ctttataatg
      421 tttactcaag aagagtgtga gtattgtata gcaataaatc caatcataga tagtgtaaat
      481 aaggaaactt tgaataagat aaagaaatat gatatatctg tagattttca atctaaacaa
      541 aagtgtaaaa ttaagtttgt tccgactatt gttgcatata gtaagaataa agaagtgggg
      601 agaattgaca atgaattaga taatttgtat cgaaaaaagc aagatggttc aattgtaata
      661 gacaagaaaa aaacaaagac attgcttata gattggatga aaaaaatgga taaacaagga
      721 gaatatttgt atggagaaga ttaaacagac atttccgtta attaataaat attggaataa
      781 gaaaaaaatt tttatatttt ttattacgtt tctgctgtca tctttatttt ctctaatttt
      841 cccgtatcta tttagtaatg ttattgattt atatttagag aatgagttat cacagaatat
      901 tgtgcgaata gtaacgattc ttatatttca gttattttta gtcttaatta aagaagtgta
      961 tggatatata aaaactattt atgcatttga agttgattta tcaatgaaaa ttgattttct
     1021 taaggtttta caaaaaaaaa atcttttttt ttcgcaaacg aagcattcag gagaaattca
     1081 atttaggatg ttttcggata tttctactat cgtaaatact atctttattt tttctgttga
     1141 attacctgtg ataggcgttt tagttcttgt agcggtattt attataagta aagtgaatct
     1201 tatttttctg attttttcac taataatgat gggagctatg gtgttacagt ataatttatt
     1261 tcaattaccg cttaagaaaa caatcgagaa tacaaaaagt gttaatcaaa gtattacagg
     1321 agaattgaat gcacattttg atcaattaga aattataagc gcatataatt ttgagaatat
     1381 ctctatttta aaattcgatt ctagatttaa agaattattt aatgttttca aaaaacaaaa
     1441 taaattacta acactgggaa gctgtttggc acaggttatt ttccaattat ggattggagg
     1501 aatatttctt attgatgtta ttttattagc taaaggaaca ataagtatta ctaattttgt
     1561 aatagtattt atgtacgcta acatggtccc tactttatgg actactttaa ttagtattat
     1621 ttggaaatgg caagactatt cagtgtcatt aaatagatat tacgaatatt attcaaataa
     1681 caacaaagtg gctggcacaa aaaaaataaa taaaataaat tctattactg gtcaatcatt
     1741 aaatttttca tataacaaaa gaaaggtatt aagcaatata agtttttcaa ctgaaatgaa
     1801 taaactattc atgatatcag gagaaaatgg aacagggaaa acaacactga tgaaattatt
     1861 tactaaacag ttgccggtta gtaataatca attattcgtc aacgggatag atattaatat
     1921 gattgataac gaatcattaa gaaggttaat ttcaatcgca acacaaatac catatataat
     1981 taatagctcc gtgaaggaga atattttaat ggggactaaa aactatgata aaaaaaaatt
     2041 cagtaaatta atagaactat tgggaataag taaatttatg acgaaattag gtattacttt
     2101 ggacgacaat atttatgatg aaaaaatatt attatctgga ggagaaaaac aaaaaataag
     2161 tttagctagg gcgcttatta aatcagcttc agttattata ttagatgaac catttagaaa
     2221 tttagataat gaaactcgcg ataatttgag ggattatata ctaaatacaa aaaaagaagt
     2281 ttgttatata attataactc atgatcacat tctagataat tttttggatt caaatgatga
     2341 aatattatat ttaaataagg agtatgataa taatgaatag caaaaaactt cagatgggtg
     2401 aaattgttag aggtcctagt caaatatcta ttgatgttac atataaatgt aattttagat
     2461 gtaaacattg ttacaatgcg agtggtggag acaattgttt tatggataat gagttgactg
     2521 atgatgaact gttagaactt acaaaagata tatgtaattt aaagccatat aatatttgtt
     2581 tctgtggagg agaaacttta ctgagaaaaa aaatagtttt atcttgtgcc actttattaa
     2641 aagaggcagg tattcctaat attgctattg ttactaatgg atcattagta actcaggatg
     2701 ttgcaaaaga attattcaaa gcaggtatta atcgatttca agtaagttta gatggaaaaa
     2761 atgctagtag ccatgaagca cttagattat atccaaacag ttttgaaaag gcaattaatg
     2821 ctatcaaatt atttagagaa ttgtccgaag aagtggatgt tgctttttgt cctactagtt
     2881 ttaattacaa tcaattacga gaagtatatc aactagtaaa tactcttaaa gtgaagtctt
     2941 taagaattca accatttatg cttagtggta ggggaatgca aaatagggaa atagtgccaa
     3001 ctgtttctca atatagaaaa ctagtttctg atattaacta tattaaaaga aatgaagatt
     3061 ataccaagat agattgggga gatccaattg atcatatcat ccgacacaga actttattga
     3121 aagatttttc gaattttgtt tctattaggt cagacggagg attaatggca tcaccttact
     3181 tgccaattca agttggaaat attaaatgtc acagtctttc taaatattgg cgagcaggac
     3241 tttataaagt tttttcttta gatttagttc aagatatagc tactcaagtt tcttcaatac
     3301 aagatatgag taaagaatta aaaaaaggac atattaaatg gcttaatgaa gatatagaaa
     3361 ttgatattct cgatgaatac aaacatgata ttttaattta ggtttatcat tggaaagttt
     3421 agataaacaa agtactttta gaaaggaggg aaaaaaatga tttctgaacc tcaaatgaat
     3481 aatatgtatg aaagcccaac tataatggga gctggtgaag ctgcgccaag agcttgttca
     3541 gcaattatgg tgtgtgtgtt attagcatct gcaggagtag gtatccaagc aggagcggtg
     3601 tatacttatg ctgtagttgc tacagtagct gtagctgcag ccgcagtttg gaaaacagta
     3661 ggattagttg gttaggaatt aataggagac taactgtctc ctattgattc caatgattaa
     3721 gctaaggagg aattttaaga aaataatgaa atacaataat ttaaaaccga aattacttaa
     3781 tgagaacata gaatatataa gagaagaaaa ttatggatat ttaaccatag ttcccaaaaa
     3841 tggaactgaa ttgaaagata acgttttcaa tccaacagta agatatatat tggctaactg
     3901 tactggaaaa aacagtgtaa aaaaaatagc gaatggt
//
LOCUS       DASJPC010000159         3875 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJPC010000159 DASJPC010000000
VERSION     DASJPC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.166"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..2257
                     /locus_tag="U4A13_002745"
     CDS             158..2257
                     /locus_tag="U4A13_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN1780993.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="U4A13_002746"
     CDS             2453..3316
                     /locus_tag="U4A13_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN1780994.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3875
                     /locus_tag="U4A13_002747"
     CDS             3421..>3875
                     /locus_tag="U4A13_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN1780995.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1543 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DASJPC010000160         3864 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJPC010000160 DASJPC010000000
VERSION     DASJPC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.167"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..135
                     /locus_tag="U4A13_002748"
     CDS             <1..135
                     /locus_tag="U4A13_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1780996.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
     gene            complement(313..2310)
                     /gene="tkt"
                     /locus_tag="U4A13_002749"
     CDS             complement(313..2310)
                     /gene="tkt"
                     /locus_tag="U4A13_002749"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN1780997.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2412..2663)
                     /locus_tag="U4A13_002750"
     CDS             complement(2412..2663)
                     /locus_tag="U4A13_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN1780998.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2810..3433
                     /gene="lexA"
                     /locus_tag="U4A13_002751"
     CDS             2810..3433
                     /gene="lexA"
                     /locus_tag="U4A13_002751"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN1780999.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3613..3831
                     /locus_tag="U4A13_002752"
     CDS             3613..3831
                     /locus_tag="U4A13_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781000.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1080 a          827 c          707 g         1250 t
ORIGIN      
        1 ttcaaccaag tcaatcaagg attcatctca tccgttgcgt ccaaaagaaa ccatatccct
       61 agaaaatcac taaattacca aacaccatta gaagtttttt tgagttacgt aaatggaaag
      121 ttttgtctcg cttaatttga caaataatat tctcaagaaa cacaaactaa tccgaagaat
      181 tctttgtttt gtcatttaaa tgtaacatag ccgaaaacga aaagtaatat tgatttattc
      241 ttctaacaca taaagaacag ctgtctccaa cttgcagaca gctgttctta tcagtcaaac
      301 aattgcttgg atctatgata gttgattgta cgtattcacg acattttcta ctgtaaatcc
      361 aaattcttct aaaattttag tgccaggggc agatgcaccg aaatgatcga ttgtaatcgt
      421 tgcgccctct gttcctacgt agcgttccca tccaaatgat gcagcagctt cgatagccac
      481 acgtttcttc actgatttag gaagtacact ttctttatat tcagaagatt gtttttcaaa
      541 gaggtcaaag cttggcatag aaacaacaga aacatctttg cctttttcag ccaattcttt
      601 ttgagctttc acagccaaat caacttcaga acctgttgca atcaaaatac cttctggtgt
      661 ttctccttgt gatggagaaa gaacatacgc gccttttttc accatttcat ctgctacttc
      721 ttttgtagaa ggcaatactg gaaggttttg acgactcaat acaaggatag taggcgcatc
      781 tgttgtctcc atcgctactt tccaagcagc acgtgtttca tttccgtctg ccggacgaat
      841 cacttgaaca ccaggcatgc agcgaacaga tgctagttgt tcaatcggtt cgtgcgttgg
      901 tccgtcttct ccgacagcaa ccgagtcatg tgttaagacg taaacaacag gagtatgttg
      961 gattgctgct aaacgaactg ctggtcttag ataatcgaca aagacaaaga atgttccacc
     1021 gtagatacga gttccgccat gaagttggat accattcata gccgaagcca ttgcaaattc
     1081 gcggacaccg aaccagatgt tgcgtccttc ataatgttca ggagtgaaat ctttatctgc
     1141 agccaccatt gtattattgg aacctgacaa gtccgctgaa ccaccccaga agcttggtat
     1201 agctttcgat aattcttgga tgacttcttt acttgaaaca cggcttgctt gactgcttcc
     1261 aacttcataa gttggcaatt ctgcatccca gttttctggt agtttgccat caaaagcatc
     1321 ttcaaattgt tgtgctaatt caggataagc ttttttataa ttggcaaaca tttcattcca
     1381 tgcatcttca gctttttgtc cttcttctat catcgtttga tggaagcggg cagctacttc
     1441 ttctggaaca gtgaaatctg gatactccca accataaact tctttagcca tcttgatgcc
     1501 atcttcacca agaggagcac catgaacagc agatgttcct tctttaggag caccatagcc
     1561 gatgactgtt tttacttcga tcaatgttgg tttgtctgtt tcagcttttg cttcttcaat
     1621 cgctttagag attgcttcta ggtcatttcc gtctttcact aagatatgtt gccagccata
     1681 tgcttcatag cgagcaccaa cgttttctgt gaaagctttt gaagttggac cgtctagtga
     1741 aatatcgttt gaatcgtata aaacgatcaa tttccctagt ttcatatgac ctgccatcga
     1801 acttgcttct tgagataccc cttccatcaa gtctccgtca ccgcatattg catacgtata
     1861 gtgatccatc acattaaagt tttctttgtt atacgttgct gcaaggtgcg cttctgccat
     1921 tgccataccg acagccattg caatcccttg tcctaatggc cctgtagtgg cttctacacc
     1981 atctgtatga tgtacttcag gatgtcctgg tgttttagaa tcccattgac ggaattgttt
     2041 caaatcatcg attgtcactt gatagccagc taaatgaagc aagctataca acattgctga
     2101 cccatgtcct gccgaaagaa cgaaacggtc gcggtcagcc cagttttttg atgtcgttgg
     2161 atttactttc aggtgttttg tccataacgc atatgccata ggtgctgcgc ccatcggcaa
     2221 tccaggatgt cctgagtttg ctttttggac tgcttcaata cttaatgtac gaattgtgtt
     2281 aacgccaagt tggtcaattt tgtcaaacaa aataacccct cctattaatt taggtttttt
     2341 ttacctttat ttcttacact ttcattctat aagatgtcac acgaaaaagc aatctttttc
     2401 agtatttttt actaaaagtt gtcatttcgg ttgtgtaagc cttttttctt ttggatttct
     2461 tttaattttt ccggggtcac gtcattgccc tcaggatcga cgactttcat tccttcgata
     2521 tgatgacgca taccggaacg aaaagcttcc aaatactctt tgcgcaagtt gatttgttct
     2581 tgttcttcgg cttttgagag accattttcc ttctttttac gtgcaagttc attgatccgc
     2641 tctatttttt caggtgacag catgctcagc cctcctttta ctctccttca tattatataa
     2701 aaactgaaaa aaaaacaact agataaacta aataaaatac gaacattcgt ttgatttagc
     2761 gctttttata tggtactatt tatttatcaa gtgaaagaag gtgtaccttg tggcccgaca
     2821 aactgaatca agacaaatcg aagtcttacg atatatccat gaacaagtaa cccaaaaagg
     2881 atacccgccg actgtccgtg aaatcgggga agctgttcag ctttcttcta cttctactgt
     2941 tcacggtcat cttgcacggc ttgaaaaaaa agggtttatc caaagagatc cgacgaaacc
     3001 tcgtgcaatc gagttgacaa aacaaggttt agaaaagatt gggattcgtc caacaactat
     3061 tccagtactt ggcgtcgtaa cagctggaga acccatttta gcagtagagg atgcttcaga
     3121 cttctttcca atccctccta atctttcttc cgaagaaggc agtctcttta tgctgacaat
     3181 tcgtggagat agtatgatca atgctgggat attggatggt gacaacgtga tcgttcggaa
     3241 gcaagaaact gctcaaaatg gcgatattgt cattgcaatg actgccgagg acgaagcgac
     3301 ttgcaaacga ttctataaag aaaaaaactt tttccgtctg cagcctgaaa acgacttgct
     3361 cgaaccaatc atcttagatc aagtatctat attaggacga gtagtaggac tttatcgtag
     3421 tcatatctat taattgctac taattaaaag aaaacttttt aaaaagagtt tgagacgttg
     3481 gtctcaaact cttttttcgc ccgggttctt cttctttcaa atttttatca cttccctctt
     3541 tacaaaccga acaaatattc gtatactaaa tatacgaaca attgttcgca taaattaaag
     3601 aggtgagaat tcatgaaatc aatgagacgt cttggcttag tattaactgt tttgttttta
     3661 ggtattatta ttggtaattt cggcatacgt ccagctttac ttgtgtttgt tccgttattt
     3721 actttgtggt tcatgttgtg ggatgaaaag aagtaccgtc aatctgagcg gcgcagaatt
     3781 catgaagatc atttctatca tgagccctac tacagatcca ggcaatcata ataaggattg
     3841 actgctaatt attcaaaaga caag
//
LOCUS       DASJPC010000161         3835 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJPC010000161 DASJPC010000000
VERSION     DASJPC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.168"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1256)
                     /locus_tag="U4A13_002753"
     CDS             complement(<1..1256)
                     /locus_tag="U4A13_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1781001.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="U4A13_002754"
     CDS             complement(1270..2487)
                     /locus_tag="U4A13_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1781002.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>3835)
                     /locus_tag="U4A13_002755"
     CDS             complement(2477..>3835)
                     /locus_tag="U4A13_002755"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN1781003.1"
                     /translation="LIATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLA
                     STYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFL
                     ISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNY
                     LTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDA
                     VVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLP
                     HGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFI
                     DASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYV
                     DTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKH
                     EQ"
BASE COUNT         1056 a          828 c          651 g         1300 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcag
//
LOCUS       DASJPC010000162         3499 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJPC010000162 DASJPC010000000
VERSION     DASJPC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.169"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(45..1511)
                     /locus_tag="U4A13_002756"
     CDS             complement(45..1511)
                     /locus_tag="U4A13_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN1781004.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="U4A13_002757"
     CDS             1898..2494
                     /locus_tag="U4A13_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1781005.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="U4A13_002758"
     CDS             complement(2552..3034)
                     /locus_tag="U4A13_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781006.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="U4A13_002759"
     CDS             complement(3140..3337)
                     /locus_tag="U4A13_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781007.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT         1182 a          703 c          578 g         1036 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaaga
//
LOCUS       DASJPC010000163         3482 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJPC010000163 DASJPC010000000
VERSION     DASJPC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.170"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(699..1313)
                     /locus_tag="U4A13_002760"
     CDS             complement(699..1313)
                     /locus_tag="U4A13_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN1781008.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1919..2296)
                     /locus_tag="U4A13_002761"
     CDS             complement(1919..2296)
                     /locus_tag="U4A13_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781009.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2596..3477)
                     /gene="galU"
                     /locus_tag="U4A13_002762"
     CDS             complement(2596..3477)
                     /gene="galU"
                     /locus_tag="U4A13_002762"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN1781010.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         1057 a          696 c          515 g         1214 t
ORIGIN      
        1 aatgacattt ccacatgaat aatttattag aaaatcactt ttttctattt tgtttttttt
       61 ctcctatgta tgattatttt tttctctttc catttatatt aagtatttta tttctctgtt
      121 tttccttaaa attctttttt tatttttaga ttagagaaaa tctcgtcctg tttttttcat
      181 tttacaatca ttgaaaaatt gaatttcatt gcaaaaaaaa taataaacac ttataaatat
      241 ccattatcta tccttcaatt tttgtgtatt ttatttctat tagaaaaagg tatcctgttg
      301 gacaattttg ttatatcagg taaacttcat tgcaataatg ataacaaatg tactataatt
      361 actttgtaaa gagttttggg gaagactctt tcatcaacgt gtattaagag acaagaaaga
      421 atccatatgg attctttctt gtctcttttt ccactacttt ttatttttct gacactacat
      481 attcttataa ctcatttctc ataactgtga aacaacttat aaaaataaca aactttaaac
      541 aaaacaagct taaaatcaaa gatgatccat cttaaaataa ctaaaacaaa aaaagtagtc
      601 gttgttaatc ctcattaata atcgttttct ttttttcaaa cgctccattt ttatgtggaa
      661 cattattttt ttaaggaact ttgtcaacat gtggataact attttccact tgtgtcctgg
      721 actagctgtt ttgctgtaaa atacaaaact tgctgcagca aaaagatcgt tgcaagagag
      781 gctgttccgc agatcgctgg atctagattt ggcaaagtca agactgcatc atgtaaattg
      841 gcgtggttgt ccaaaaccaa atcagcaaat tcaaataatc gcaaaccact gctatgttgc
      901 gatttgatac ttcgtgacaa atcaaatcca gtaacaatga ttaatggatg accattttct
      961 ttgatccact gagctagttc gatgattgcc gggtttcttc cttcattcga aagaagaaga
     1021 aaaatgtctt ctgatttgac agaaagctga tcaataattg cctgtcctgc accttctagc
     1081 gtttcataga cacctccggc tggattttca atcaatatac ttggtacaag atccggggtt
     1141 tgatcccaaa attcaaatgc tacaccactt agtgtgcggc tagcatatat ctgcaatcta
     1201 ccaccttgat taatttgttt cgcgatccaa gtgccagctt gaagcatcag cggctcttct
     1261 atccttaatt tctcttccaa ttggtgataa tagtcaaaat aacataaact catttatctc
     1321 tcccgcctag ctatacttga catgaataga aacagctgcc taatctaagg aagtacataa
     1381 aaaaacagta gttagacagt aacacatgtg gtctttgctg tttcaaacac aaaaaaggca
     1441 ttgaacgaat acgcccaata ccttgacata gcaaataaaa caacgtgcag accatggaaa
     1501 atatctacat aggggtagaa ttttcaccct agattaggta caataaatta ggtcgatgca
     1561 ctgcattgac tatggcaaaa tgatcggtca gggtgtatgt gtgtgactgg tcattccctg
     1621 attcttagcg ccaactaagg gtcagggagc ctttttttat tcagctgttt ctatctgaat
     1681 ttattgtaag taatctctta tccaagtgca agcaagaaaa tacaaaaaaa cattggtatg
     1741 atttatatat atataaataa cataaaaaaa ggaatggctt atcccgatac aacaagcatt
     1801 cctttaaaaa agattctttt atataagtga aaaaagcttt tttccaccaa ttaaataaat
     1861 ctctcatctc aatagtttat ccattatttt atcttttttt gcgtatacct actaaatatt
     1921 accgtcttct tctcgttctt ctactttgtt cgagagaatc tgtactagaa aaagtagttg
     1981 tatgtctacc ttttctgcct atccgagact ttcttttcat atatgggata taaatgatta
     2041 gatacagtac agcaaaaaat aaagcaaata cgcaagcttc ccaactacct aacgccaatt
     2101 caaccatcaa ggtagcaacc ccaacggatg ctgcatacca gataaaaatc agaaacgttt
     2161 ctttttttct gaccatgcga aacatgataa atagactaat aagcaatagt ggcaacacaa
     2221 atatccaatt gcttccttcc atcagccact tgagtatatc aagcagcgac tgtgcaacag
     2281 cgactaattt tcccatagtg aacacacacc tttttttata attaaaaaat tcgccttttt
     2341 attctactat gaatcttctg gcttggcttt catttctctt atgtaatcat tttcttttat
     2401 acaacttaaa aaaaaattaa accattagca taatattttc tgaatctatt cacgaaagtc
     2461 tttttctatc tttcgttttc cacgactcaa ctgatctaga gtcaaaaaac taaaacgaca
     2521 gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa tagcctttca ttctcagcag
     2581 gccacttatt tccagttaac tttctgttct tagcttttta ctcaactgga taatatatgc
     2641 acttaatgaa gtcccaattt ccgaatgttg aagtccatat tcaatatttg tcttcatata
     2701 tcctaactta tcgcctacgt cgtatctttt tcctttaaac tctctcgcat agaccgcctc
     2761 tgtctggtta agagtctgaa gtgcatccgt tagctgtatc tcgtttcctg ctcctggttc
     2821 ttgagtttct aatatctcaa atattttagg agtcagtaga taacgtccga taatggctaa
     2881 attacttggt gcttttccca tttcaggttt ttctacaaac ccttttactc tgcaacgacc
     2941 cttttcgcta tctgttttag gatcaataat tccgtattta gatgtttcat tttctggtac
     3001 acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa tccatcatca attgttgagt
     3061 caatgggata tcatctttca tcaaatcatc ccccagcatg acgacaaacg gttcatttcc
     3121 tataaatgct tttgcttgaa gcacggcatc ccccagtcct cttggatgag attgacggat
     3181 gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg agtaattctg ttttttcctt
     3241 ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag tgatcttcaa tgggtctttt
     3301 ttcttttccc gttacgatca agatgtcttc gattcccgaa tccaatgctt cttccacaat
     3361 aaactggatc gttggtttat ccacgattgg aagcatttct tttgccatgg cttttgttgc
     3421 cggcaaaaaa cgtgttccta gcccagcggc aggaatgact gcttttttga ccttcacttg
     3481 ct
//
LOCUS       DASJPC010000164         3388 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJPC010000164 DASJPC010000000
VERSION     DASJPC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.172"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..248
                     /locus_tag="U4A13_002763"
     CDS             <1..248
                     /locus_tag="U4A13_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1781011.1"
                     /translation="FTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEK
                     FIFDKTDKEIGKVLGITRQGATNLKHRLYKKIQKMLV"
     gene            342..533
                     /locus_tag="U4A13_002764"
     CDS             342..533
                     /locus_tag="U4A13_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1781012.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1047..1379
                     /locus_tag="U4A13_002765"
     CDS             1047..1379
                     /locus_tag="U4A13_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781013.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1289..1483
                     /locus_tag="U4A13_002766"
     CDS             1289..1483
                     /locus_tag="U4A13_002766"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1781014.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            1675..1830
                     /locus_tag="U4A13_002767"
     CDS             1675..1830
                     /locus_tag="U4A13_002767"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1781015.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2106..3388
                     /locus_tag="U4A13_002768"
                     /pseudo
     CDS             2106..3388
                     /locus_tag="U4A13_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         1169 a          622 c          607 g          990 t
ORIGIN      
        1 tgtttactaa agatactata aaatttaaac aaccgattaa tgttttaaat gtgactctcc
       61 ttgttgaaga tataaaatta gcaaaaatcc ttcctttgtt gaaagaacct gaacaaattt
      121 ttcttatcga aaaatttatc tttgataaaa ctgataaaga aattggaaag gttctgggga
      181 ttacaagaca gggtgcgaca aatttaaaac atcgacttta caaaaaaatt caaaaaatgc
      241 tggtttaata tctgcataat aactaatttt gtatgcttct ttgctcgttc tcctccttcc
      301 caaagaaata gattataagt ccatgggaag aaggtagatt tatgaattct ttaaaagaag
      361 atttcatttt agcaaaggct ggaaatgaag aagctgttga ggcaattctc aaaagattta
      421 gctctttgat tcataagcaa tcctggagaa ctggaaaata tgatcaagat tgttatcaag
      481 aatgtatgct tgcaatatat ctagctatct cgaaattcga aattaaagaa taactcaact
      541 aagggagtct gtgacataag taaaaagata gtttcaaatt tcgaacaata aatttgaaac
      601 tatcttcttt ataattataa agtatcgatt ttacagcgtt ctataatgat tcatagaatc
      661 taaaacgaat gattttaatt atcaacgttt tttaattcgt ttttagctta aaaataactt
      721 ttgtcacagt ctcactccta aaattacttt atttacgtat gaagtgggca ggaggtcgaa
      781 tccagaactg ggctatggta cttcaacgag gacttctcct ctagaattga aaagcatgcg
      841 atttaccttc cgtaacgacg gtagatttat tcaactatgt gtccaatcaa aatttgtctg
      901 atttcaaaaa tagtcaagag aattttggtt gttaaaaact cttatctatc aacttccaca
      961 gtttagttga caaaccctga tactaagttg ggctatgata cacttttctt gactatcagt
     1021 tacgttaccg gaaggaatga actctattga gaaaaacata tgatcgtaaa tttaaactaa
     1081 agatttcaca ggatatcctt gagaaaaaag taactaccaa gaaaattgct gaagaataca
     1141 atatctctcg tccaacaatt tcccgatggg tttcagaata tcgtcgatac gggaaaaatg
     1201 cttttgctgg acaaggaaaa agattacctg ataaagcaga tttctacatt cttgaacagg
     1261 aaaataaacg actaaaagag gaagacgagt gttctacgaa cacaaagatc gatacggatc
     1321 tccagggatc actcaaaaac tttacgatga aggaatcgaa atcaataaac gtgttgtagc
     1381 tgttcttatg cgtgaaatga atttatgcac aagaggattt catcgacgca aatcttctca
     1441 tggtagagaa aaaacaatca aagaaatggt aaaaaaatgt tgaatcgata atttgaacag
     1501 aaccaaattg atgctgtttg ggtgatggat attacttaca ttccatgtaa tgacggtcgc
     1561 ctctatttgt ccacctatat tgacttagca actcgtattc tacgttacta tatggtagat
     1621 tcacacatga aaaaagagat tgtcgtccgt ccattacaaa tttattaagg agaattgcct
     1681 acggtaattc attccgatag aggtagccag catcggtctt ttatttatca agagctgttg
     1741 aacgaccacc acatcacaca ttcagtgagc caaactggga ctcctgttga caatgcagta
     1801 attgaagcgt ttcatcgatc aatcaaatga gaattaatcg actccaacaa atataaaagt
     1861 aaaatagaga tgaaggtgtt aatccaagat tatttagaag actactaccc aaacaaaaga
     1921 atccatacaa aatttatgat gactccttga caatttgaag cgaagaaaat aagggatcta
     1981 taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt ctaataaacg
     2041 caaacagaca ccttaaaatc ctttagaata aagtcgacga aaaccattca aaggagtgtt
     2101 tcaagatgtc ctattcagag tttaccaaag agttactaga tattctagac ctaaatctta
     2161 tctttcatga agatgccttt cgcaaagagc gaatcaatga cgaaacatgt tttgtttttg
     2221 atggcacatt gacctatcaa ccggaggaat gctttcatcg ccactatcaa aatgaacaaa
     2281 cgatcattaa atagggctgg aaaaaagtat ccatcttatt gaatgatgtc agtaactaca
     2341 agaccattct gcggattaac aaacagcgct tcaaatgtaa acactgtgga aagacttttt
     2401 tagcagaaga ttcagttagt gatcgtcgct gctccattgc tcgcagagtc aaacaagcca
     2461 ttcttgagct attaagcgaa cctctttcta tgtctttgat cgcacgtatg aagcacattt
     2521 ctccgaccac tgtcattcga atacttcgca gccttcggcc taaaaccatt tctttgagcc
     2581 agctgttacc agaagtggtg tgtttcgatg aattcaaatc agtgaaaaat gtctcgggag
     2641 ccatgagctt tgtcatgatg gacggcaaga cccatcaact gattgatatt gtggaaaatc
     2701 ggcaactcaa tcctttacgc gactatttct tgcgctaccc tataaaagtc cgtgaacaag
     2761 tgcgtctagt ggtttcagat ttctatacac cctatcgcac attggtgaaa gagtgttttc
     2821 cgaatgcccg aatcgtcgcc gatcgctttc atattagcca acacattggt cgggctttta
     2881 ccaaccaccg aattcaagta atgaagacct ttaaaaaagg ggatcgtcgg tcgaagcact
     2941 taaagaaata ctggaaacta cttcaaaaga atgcgtggga attgaaaggt cagcatcgct
     3001 actggcgtcc gtcatttcga gatcatctga cagaagcaga gatagtcgat cggctattat
     3061 cttatgatga ttccttaaag cgaggctacg aagtctacca ggattttctt tccgttatca
     3121 gaagacaaga tgttcctgaa ttcgtcgcgc tcctaaaaga agactacaag gagttgccag
     3181 agcactacca accagtgttc acgaccttca agaaatacca aacagaaatc aaacgagcgt
     3241 tgcgcgttcc ctattccaac ggccctatag aatgtttgaa taaccatatc aaagtactaa
     3301 aacgaattgc ctatggctat agaaactttc aaaactaccg agaacgaatc tttttatatc
     3361 gaggaaaata ttttaagaaa actaagaa
//
LOCUS       DASJPC010000165         3273 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJPC010000165 DASJPC010000000
VERSION     DASJPC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.173"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..692
                     /locus_tag="U4A13_002769"
     CDS             <1..692
                     /locus_tag="U4A13_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1781016.1"
                     /translation="DESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFTAI
                     LVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVGKF
                     IRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGYAQ
                     VYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVISLA
                     EIAFPNEKIFR"
     gene            708..1748
                     /locus_tag="U4A13_002770"
     CDS             708..1748
                     /locus_tag="U4A13_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1781017.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            1767..2852
                     /locus_tag="U4A13_002771"
     CDS             1767..2852
                     /locus_tag="U4A13_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1781018.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            2885..>3273
                     /locus_tag="U4A13_002772"
     CDS             2885..>3273
                     /locus_tag="U4A13_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1781019.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKH"
BASE COUNT         1204 a          447 c          611 g         1011 t
ORIGIN      
        1 aagatgaaag tattattgaa gcatctggat ttagaatcca acctgaatat gaactattca
       61 aacgtttgtt tgatatcatc gtttcacttg ttttattagt tattgcttca ccatttatgt
      121 tattcacagc aattttagtc aaagtgacat caccgggacc aatcatctat aaacaagtgc
      181 ggatcacaaa aaatcaaaag gaattttcaa tctataaatt ccgctcgatg accgccacag
      241 ccgaagcaaa atctggacca gttttagcta agagcaatga tgcacgagta acgcctgtag
      301 gtaaatttat tcgagctgtc cgctttgatg aattaccaca aatcttcaat gtgctgaaag
      361 gcgatatgtc gatcgtagga ccacgtcctg aacgttcttt cttcgtcgat cagttcaatg
      421 cagaaaaccc ttattactac ttacggcata atgttcgtgc tggtatcact gggtatgcac
      481 aagtctatgg aaaatatgtc tctgattaca acagtaaatt acgttttgat ttactttata
      541 tcaaaaaata ttcattgatg cttgatttaa agatcttgct gcaaaccatc aagatcctat
      601 ttgataaaat gtcctctcaa ggacttgaag aagacgaaaa tgtgatctca ttggcagaaa
      661 ttgcttttcc gaatgaaaaa atctttcgct agttgggagg cgtaaaaatg aacaatccga
      721 aattcagcat aatcgtacca gtatacaatg cagaagcaac tattggtcga acgattgata
      781 ttctaaaaga attggattat gaagagtacg aagtcgtttt agtaaatgat ggatcaacag
      841 atcaaacaca ggaaatcatt caagaactga ttgaaaatga tgaacgtttt gaattgatta
      901 ctacgaccaa tcaagggccc ggattagcaa gaaacgaagg aataagacag tcaaaaggag
      961 aatatctctt attcttcgat gcagatgaca cgccagtaaa aacgatcttg tcagattatg
     1021 atcatttgct agaaaagagt ccaaaagcgg atcttgttgt tagttcgttt gtttttcgta
     1081 caatggacga gggaagaatc gtttccgaaa aagaaaactt agttacagaa tgtcactatc
     1141 taactcacga agagtttata aaagacatgt atcagttaat gaatgaacag ttgatgtatg
     1201 ttgtatggaa caaatgctat cgtagcgata ttctgaaaaa taaccaaatt ctatttaaaa
     1261 cttataatag ttgtgaggat cgaatattta acttacatta ttatgaatac tgtcatcaag
     1321 tgatcatgaa cccaaaagta gaatatattt atgaatttga aggtgggaag gggatcacta
     1381 atcaataccg acctaacaag ttcgaaacat ttaaagaatt ctattcatta gcaaatactg
     1441 taacagacga tattaataaa gacggtatgg cggcgcttct tttaaaaggg acaacttcag
     1501 ttattttttc tatttatagt acctctgcaa gaacttcaaa agagaagaaa gacgaagcaa
     1561 agcagatatt gagtgatcca gtgatccgag aagccaagca aattgctaag acggattctt
     1621 cagcaaaaaa aataacaaag ttgctttaca atatgccagc tccactattt ttatctgctg
     1681 taaaagttgg aagcttcgtt gaagtgaaaa tgccagggtt aatggctgct ttgaagcgag
     1741 tatattagtt aatggaggaa aatataatga aaagaatttt gcattttcag ggacgaatgg
     1801 gtttaggtgg agcagagtct ttcatgatga atttatatcg taaaattgat cgtacaaact
     1861 atcaatttga ttttttgatt tatgaagatt atgcagatgt tcaagactat cattcagaga
     1921 ttgaaagatt aggaggccgt atttttgttg tacctaatcc taagaaaaat atttttaaat
     1981 atttaataga agtgaataag ttactaaaaa aagaatcttt ttctatagtt cacaatcagg
     2041 tttattttgg cgggggaatt aatttatggt tagccaaaaa gaatggaatc agacagagaa
     2101 ttgctcatag ccatgcaact gaagatggaa aatctcaaaa tattgtaatg aatgtattgc
     2161 gtaaattttt aaccaaattg ttgttacaaa atgcaacaga ctaccttgcc gttagtcaag
     2221 aagctggtga aagtctgttt caaaaccatc cctttgagat agttcataat ggtattgatt
     2281 tagagcttta tagtaaaaat tcagaagcta aggttaataa gagaaaagaa ttggatatct
     2341 caatgagtac ttttgttgtg ggaaacattg gacgattaga gaaacaaaaa aatcaatctt
     2401 atttgttaga gattttttta gaaatactaa aaatgaattc tgattcttac ttgattttag
     2461 taggtgaagg gtcacttaga aataaactga aagccaaagc agtagaattg gatatagaga
     2521 aaagagtaaa atttttagga gaaagaaacg atattcccga gttattatct gtgatggatg
     2581 tttttgtaat gccttctctt tatgaaggtc tgccaatggt tggattagaa gcacaagcag
     2641 caaaattaaa attagtctta gcagatacta tttccaaaga tacaaagtta acaaataatg
     2701 tggattttgt atcattagat gaaacaccac aagaatgggc aaaaaaagta ataaaagagc
     2761 cgtatgctaa taaaataaca caagaactaa agagttatga tgtttcgtac actactgaac
     2821 aaatggagag tatatatagt agagatcatt aaaaatgttc aagatatttt aggagtgatt
     2881 atttttgaaa atatgttatt tgatattggc atacaatgat attggcaact taaagagatt
     2941 ggtcaatcga ttgaacgaaa atgcagtttt tattattcat atagataaaa aaactgatat
     3001 agaaccattt attgaagcat ttaaatcata tgataatgtt gaatttttat gtaataaaaa
     3061 ccgtgttaaa atttattggg gtggtttttc cattgtacaa gcagaaatca atttagtaaa
     3121 gagagcatta caaaatgaga aatatttaaa atatgtatta ttatctggtg ctgattatcc
     3181 aatcaaagat aatgagtata tttataatta ttttaaaaag aatagttcag tggagtttat
     3241 tcgtggaata gatctagatc aaattaaaca taa
//
LOCUS       DASJPC010000166         3221 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJPC010000166 DASJPC010000000
VERSION     DASJPC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.174"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            301..585
                     /locus_tag="U4A13_002773"
     CDS             301..585
                     /locus_tag="U4A13_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781020.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="U4A13_002774"
     CDS             588..1004
                     /locus_tag="U4A13_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781021.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="U4A13_002775"
     CDS             complement(1141..1860)
                     /locus_tag="U4A13_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1781022.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="U4A13_002776"
     CDS             2535..2630
                     /locus_tag="U4A13_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN1781023.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="U4A13_002777"
     CDS             complement(2932..3177)
                     /locus_tag="U4A13_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781024.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DASJPC010000167         3085 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJPC010000167 DASJPC010000000
VERSION     DASJPC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.175"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1008)
                     /gene="murB"
                     /locus_tag="U4A13_002778"
     CDS             complement(73..1008)
                     /gene="murB"
                     /locus_tag="U4A13_002778"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN1781025.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1081..2001)
                     /locus_tag="U4A13_002779"
     CDS             complement(1081..2001)
                     /locus_tag="U4A13_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1781026.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2114..2866
                     /gene="xth"
                     /locus_tag="U4A13_002780"
     CDS             2114..2866
                     /gene="xth"
                     /locus_tag="U4A13_002780"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN1781027.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          871 a          628 c          535 g         1051 t
ORIGIN      
        1 cgtaaacaac cacaaaaaag agcaggacgg ccaccgtcct gctcttttac ttattttaag
       61 ttccattact tattaattgg ctaaacttct gcttgtttct gatattgctt gtttttctcc
      121 aatgatccgc acttctgttt ctaaagacac atcaaatttt tctttgatta cttcttggat
      181 gtgcgcaatc aattcaatat aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc
      241 agcatgtttt tcagagactt gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg
      301 gatcagctga cctgtatagt gtcctactgg gcgcttaaag acacttccgc atgaagggta
      361 ttctaatggc tgttttgatt gacgcaattc ggtcaactca tccattcttg ctttgattgc
      421 ttcataatcc cctgtttgca aactgaatcg agcttccaat acgatagcac gtaattcttg
      481 gactttactg tgacgataag aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc
      541 atcttcaagc aaaagatcca cttcagcaaa aacatctttg atctctccgc catacgcacc
      601 tgcattcata taaaccgctc caccgacact tcccgggatg ccgcaagcaa attcaaatcc
      661 agtcaatgat tctgccaaag ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc
      721 aatgaccatc gttcctttga catgaatctg tttcatttca gtcagcatga tgactacacc
      781 gcgaatcccg ccatcttgaa cgatcagatt gcttgcattt cctaatacca tccaaggtat
      841 atcatggatt cggcaataat cgatgatttg ctttacttct tcttgttttt tcgggaatgc
      901 aaggacatca gctggtccac ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa
      961 caatatagta atcgtggcta agctttcaac tatttcttct ttattcactg aggattttcc
     1021 tttcttttct caaacgtcct cattattcta tcacgcttaa tacaatcttg ccagtcttgt
     1081 tcaaagttga tgacggtagt gatctttaag cacttcctca cataattcat gacttcttac
     1141 aacttctttt tgtttaagca attctgtttc cgatttacct aaacggatct gagaaaagaa
     1201 ggcctctacc attggctcaa acccgcgttt gactagcgtg tttgtccagt cgccaaattc
     1261 tttcactttt tttccatcag cctcttgaat gacaagctgg gtcagatcgt ttacttcata
     1321 agtaccattt ttacttgtta cttgatattt ttctatatta gcgccacttg ctaaatccat
     1381 cgttaaaata gccgtttgat tttgtgtttc taattgtaaa atggcatatt ccaaaaattc
     1441 ctttgtttct cttatctggt attttgttct caaaatgggt tcatctaata aatacacagc
     1501 tgtatcaata agatgtaaaa acaaatcata aaggacgaac tctgtcgttt cttttgccgc
     1561 tattcgattt ttttgcaggt ggatcaaccg cttatctggg atacgtttca attcttctac
     1621 cataggagca aagcgccggt tgaaaccgat catcaaaatt atttggttct tatcagctaa
     1681 ttcttgcaat tcacgaactt catttagatg gacactcagt ggtttgtcaa tataaacatg
     1741 gataccattt tctagacatt ttttggctaa ttcataatgt acttttgttg ctgcgtggat
     1801 catacatgct tcgatttttt cttctaaaag ctcatcgatc gtttcatata gatgttggaa
     1861 cccgtactct ttttgaattc tttcttttgt ttctttattt cttgttgcaa aataaaaatc
     1921 aagtgttcct tgttttttcg cataagttgg taaatatgct ttttctgcga tattccctac
     1981 accgattaca ccaatcttca tctactttcc tcctggatca tttagttact ttcatcttac
     2041 accatttttt actaaaatga ggtacaatag tatcgcaaag gtaaggaaat taattcgaag
     2101 gagtgtgctt ttattgaaat ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa
     2161 taaaaatttt ttagaagttt ttcatgagtt agacgccgat tttttctgtc tgcaggaaac
     2221 gaaactccaa gcaggacaaa ttgatttaga tcttccaggt tattatcaat attggaatta
     2281 cgcggaacga aaaggctatt ccggcacagc gatttttgct aaaaaaccag cattgaatgc
     2341 cacttacgga atgggtatcg atatccatga tacagaaggc cgtctgatta ctttggaata
     2401 ttccgatttc tttcttgtaa cctgttacac gccaaattct caaagcgaat taaaacgttt
     2461 agattaccga ttagaatggg aagaagcctt ttataattat ttggaaaatc taaaaaaaca
     2521 aaaaccagtc atcgtgtgtg gggatctaaa tgtcgctcat caaaagattg atttgaaaaa
     2581 ctggaaaacg aatcaaaaaa atgctggatt cactccagaa gaaagagcgg ctctttctcg
     2641 actgttggat aacgggttta tcgatacatt ccgttatttt tatccaaccc aggaaggtgt
     2701 ctactcttgg tggaactacc gattcaattc aaggaaaaac aatgctggtt ggcggatcga
     2761 ttactttttg acgagcaaag atttagagcc aggactagct gatgcaaaga tccatacgga
     2821 cattatgggc agtgatcatt gtccagtaga attagatttg aaataaagag gttgtgaaaa
     2881 agtcgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca cattttgcat
     2941 aaatttggca taataccgag aaactggctt ttgaacactg tttatcgaga gattgtgaaa
     3001 aagctgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc atatttttcg
     3061 tttggttggg cttattaccg cagat
//
LOCUS       DASJPC010000168         3054 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJPC010000168 DASJPC010000000
VERSION     DASJPC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.176"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1..354)
                     /locus_tag="U4A13_002781"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="U4A13_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="U4A13_002782"
     CDS             complement(614..1162)
                     /locus_tag="U4A13_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781028.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="U4A13_002783"
     CDS             complement(1163..2020)
                     /locus_tag="U4A13_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN1781029.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="U4A13_002784"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="U4A13_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="U4A13_002785"
     CDS             complement(2582..2911)
                     /locus_tag="U4A13_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1781030.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          893 a          620 c          384 g         1157 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac ctta
//
LOCUS       DASJPC010000169         3023 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJPC010000169 DASJPC010000000
VERSION     DASJPC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.177"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1243
                     /locus_tag="U4A13_002786"
     CDS             <1..1243
                     /locus_tag="U4A13_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1781031.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="U4A13_002787"
     CDS             1256..1888
                     /locus_tag="U4A13_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1781032.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="U4A13_002788"
     CDS             2093..2320
                     /locus_tag="U4A13_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEN1781033.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="U4A13_002789"
     CDS             2389..2655
                     /locus_tag="U4A13_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN1781034.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="U4A13_002790"
     CDS             2645..2914
                     /locus_tag="U4A13_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1781035.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          901 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt ttt
//
LOCUS       DASJPC010000170         3013 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJPC010000170 DASJPC010000000
VERSION     DASJPC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.178"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            177..359
                     /locus_tag="U4A13_002791"
     CDS             177..359
                     /locus_tag="U4A13_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN1781036.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="U4A13_002792"
     CDS             462..746
                     /locus_tag="U4A13_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781037.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="U4A13_002793"
     CDS             762..998
                     /locus_tag="U4A13_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781038.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="U4A13_002794"
     CDS             1020..1274
                     /locus_tag="U4A13_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781039.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="U4A13_002795"
     CDS             1356..1541
                     /locus_tag="U4A13_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEN1781040.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="U4A13_002796"
     CDS             1608..2003
                     /locus_tag="U4A13_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN1781041.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="U4A13_002797"
     CDS             1993..2370
                     /locus_tag="U4A13_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781042.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="U4A13_002798"
     CDS             2392..2766
                     /locus_tag="U4A13_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781043.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DASJPC010000171         2826 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJPC010000171 DASJPC010000000
VERSION     DASJPC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.179"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            798..1052
                     /locus_tag="U4A13_002799"
                     /pseudo
     CDS             798..1052
                     /locus_tag="U4A13_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="U4A13_002800"
     CDS             1122..1634
                     /locus_tag="U4A13_002800"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781044.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="U4A13_002801"
     CDS             1631..2668
                     /locus_tag="U4A13_002801"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1781045.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DASJPC010000172         2806 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJPC010000172 DASJPC010000000
VERSION     DASJPC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.180"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            384..572
                     /locus_tag="U4A13_002802"
     CDS             384..572
                     /locus_tag="U4A13_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN1781046.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            650..1663
                     /locus_tag="U4A13_002803"
     CDS             650..1663
                     /locus_tag="U4A13_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN1781047.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1730..2764)
                     /locus_tag="U4A13_002804"
     CDS             complement(1730..2764)
                     /locus_tag="U4A13_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN1781048.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT          872 a          536 c          546 g          852 t
ORIGIN      
        1 tagttagtct acattatttt ggcgatttgc caataaggac tgttgagttg tgcaaaatca
       61 aatctgggtc agaatgaacg tcattctgtc tcagattttt cgttatttca atttgattaa
      121 ctgattgatc aaaaagattt ttattttcat attctcaaat gattcaacac taaatataca
      181 tgtttgacgc ggaaattatt attcgatttt ggcggcatca ggggggatga cgacgtagca
      241 gacccattat tttactcaag aaaatgtccg tgcctattat ttagaatacg atagtgaacg
      301 tgctgggagt tttgttcttt tagagaaagt ctctgaagat aaattagttg tcttagattt
      361 tgactgtaac gaaaaatggc gaaatggaag agcaccagca agtgattgac cggatctacg
      421 aagcgacacg atatctgcct ttggatcgtt tgtgcttaag tcctcaatgt ggatttgctt
      481 caacagaaga agaaaatgtt ttgtttgaag aacaatggaa aaaatttctt ttgtcaaaag
      541 cattgcagaa gaagtttggg gaaaatcatt aaatcatcta ggagagcttg ataggaaata
      601 acactatggt ataatcagaa tgcttaatac taactactga gggagcctta tgaaaaagaa
      661 aattcttaaa tttatcggat ggttcttcgg aatcattctt gggatcgcgt ttgcaatctt
      721 tcttgccttc cagctttccc ccagacctgg agcattcttg atcaaccata tgttttcaaa
      781 tactgttcaa atcactgata aaaaaacgtt cgatcaagca gaaaaaaacg tggcgaaaaa
      841 aacagatttg acgtatgagt ccaagtataa agagaatact tatgacatct actatccaaa
      901 aaacagcaag gggcctgttc ctgttctatt ttgggtacat ggtggaggat tcgtcggcgg
      961 agataaatcg ggtgtcaaag aatttgcgac aaaattagtt tcagacgcca acatcgcagt
     1021 ggttgcgatg aattatgaat tagcgcctga ttcagaatac cctaatcaag ttcttcaagt
     1081 gaatgaactg atcaaagatt tattagctga agaaaaagac gacaaattgc tagacatgga
     1141 acaattattt tttggtggag atagtgcagg ttcacaaatc gcattgcaat atgcagctat
     1201 acaaacgaat gatgcgtatg ctaaacaaat gaaaatcaaa cagctacttc caaaagatag
     1261 tctgaaagga accatttctt attggggacc tgttgatttg aagctaacag cacagcagca
     1321 ttcggatgat cgatttatga agtttttcgt caaaaccgtt gcgtggtcgt tgattggaac
     1381 aaaagattgg aaaacgagtg agcaattgaa agaagctagt gtggctgacc atgtttctaa
     1441 ggaatttccg ccaacgtata taactgacgg aaatgcatac tcattccagg agcaaggtca
     1501 agcattagaa aatcggttga cagaattaaa ggtacccgtg caatccttgt tttatacaga
     1561 tacaaaaaaa gagattaccc atgaatacca atttgattac acgctaaaag aatcgcaaaa
     1621 ttgttaccag cagacattgg attttgtgaa taagtataag taagaggttg tgaaaaagcc
     1681 gtttcgtttt gagtatgaag aaacgaagcg gcttttcaca acctctcttt caataccttt
     1741 ttgttcttgg gataaaactt tccctaacaa tcaatcgtgc aggataagtc aaaatgattg
     1801 gttcgtcccg tataccgtca attcgctcca atgccattcg aacagctgct tgcccaattg
     1861 atttcgcatt gatttgaaca ctggtcaagc caggggagag gtttgcgatt gtctcgatat
     1921 tatcaaagct tgctacaaca atctcttctc caatttggat atttttttgt ttcaatgccc
     1981 gatagacacc aattgccatt ggatcgctcc cgacaagaat ggcagtagga agaggggatt
     2041 cattttctaa tagattagtc gcaagtacct caccttgttt ttctgtccag ccacctagat
     2101 gataagacat atatttttct aagtgatgat cgatcataaa atgctgatag gcacgcatcc
     2161 tcttttcatt tatagaaatc tgcttcccac cgaattcatc catatcgact ttatatcccc
     2221 cgatataggc tattctttta tgaccatttt ctaagaacaa ttccagtacg gttttagtca
     2281 tccgttcaag atctgcatac accatatcga cctcttgctg gatgtcaggg ttatccacga
     2341 cgactatttg catgttttgt tttaacaagg cggtaatcga ttctttcgta acagtcccaa
     2401 caacgatcac cgcacctaac tgatctagat cttcccattg tttaggatta tcagatagat
     2461 tatagattcg attcatccct aaatgcagac gtcgagcttc ttcttccatc cctactcgca
     2521 aatgtttgta atagatatcc tgttcttcgc tcgctttgcc gatcgtagag atcacaccaa
     2581 tcgaaggcat tctctttttg atatatttac gagtactgat gtcataatcc aacgcagcag
     2641 ctgcctctac tatccgttgc cgagtagtct gagccacatg gatcgtctga tcgttattca
     2701 acacacggga aactgtagca ggagagacat ttgcctttcg tgcgacatct ctaattccaa
     2761 ccatttttac acactcctca aaaaaataaa aaatttttat tgacaa
//
LOCUS       DASJPC010000173         2753 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJPC010000173 DASJPC010000000
VERSION     DASJPC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.181"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            231..1238
                     /locus_tag="U4A13_002805"
     CDS             231..1238
                     /locus_tag="U4A13_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN1781049.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1250..2254
                     /gene="neuB"
                     /locus_tag="U4A13_002806"
     CDS             1250..2254
                     /gene="neuB"
                     /locus_tag="U4A13_002806"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEN1781050.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2251..>2753
                     /locus_tag="U4A13_002807"
     CDS             2251..>2753
                     /locus_tag="U4A13_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEN1781051.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRV"
BASE COUNT         1015 a          345 c          530 g          863 t
ORIGIN      
        1 ttcattataa tatcgctaaa aaaattacga ataaacaatt atttgacaca aaaaaaatat
       61 gtatcataat aatttttgta tcaatttttt ctttgatact tcatatttta atcaatcaaa
      121 tgttgattcg ttacgttttg acatcattat tgataattag tttagttata tattttttaa
      181 agattaagaa tcgaattata gatgactata attcatagga ggagatataa gtgtttaaag
      241 gaaaaacaat attagtaact ggcggaactg gttccattgg aagtgaaatt gttcgtcagc
      301 ttttaaaata tgaaccaaaa gctatcagaa tttatagtag aggtgaagaa aaacaatttt
      361 atatgcaaca agagctctca aatcacacta atttgagatt tttaattggt gatatcagag
      421 actctcaaag gattgattat gcatgtcgtg atgtggatat tatttttcat gctgccgcaa
      481 tgaaacacgt tccagcaagt gaatataatc ctatggaagc tgtaaaaaca aatgtgatgg
      541 gaactcaaaa tgtaatagat gcagcgatca aaaataatgt aaaacatttt gtaggtatca
      601 gtacagataa agtagtgagt cctactaaca ccatgggtgc gacaaagtta ttgagtgaaa
      661 aattaataat agctgccaat cagtataaag gaaaaacaga aacaatattt tcttgtgtaa
      721 ggtttggaaa tgtcatgggg tcaagtggat ctgttattcc tctttttagt aaacaaataa
      781 aaaacaataa agatattact ttgacagatg ttgatatgac acgatttatg atgtcaatac
      841 cggatgctgc aagattagtt ctgtctgctg ctatgaattc aaaatttgga gaaacatatg
      901 tcttaaaaat gccggcaatc aatattttaa ctttagctca atcattaata aaaatatata
      961 atgatacaca tgatgaaaaa tatagaggtg aaataaaagt tgtaggtatc aggccaggag
     1021 aaaaattata tgaagagcta atgactacag acgaaattga acgctcatat gaaaatgata
     1081 aaatgtatat cattccacca cactatcatg ctactgacga actgtacaaa gattatgaaa
     1141 aaattaaaat cactgcatac tcttcagatg caacagaatt aatgagtgga caggaattta
     1201 ttagaatcgc aaaagagaat aatgtcgtga cttggtaagg agttttatga tgaaatcaga
     1261 agtatatgta attgcagaaa ttggtgtaaa ccataatgga agtctagatt tagctttaga
     1321 aagtattgat aaagctaaag aatgtggagc taacgcggtt aaatttcaaa catttaaaac
     1381 gaataagcta gtttccaaaa aggctccaat ggcaaaatat cagaaggata atcttaaaga
     1441 ggacagttct caatttgaaa tgctaaaaaa attagaatta tctcaatctg attttattag
     1501 aataaaagat tattgtatac aaaataaaat tgattttttg tctactcctt ttgatgaaga
     1561 aagtgcagac tttctaaaac aaattggtgt acaaggattt aaaattggct caggagattt
     1621 aacaaattta ccttttctta gaaaaataga tatgtatggg ttgccagttt tattatctac
     1681 aggtatggct gatattcatg aggtcagtga ggctgtagat tgtttcatag atagtccagt
     1741 tactatttta cattgtactt caaattatcc ggctagttct gaagatatta atttattggc
     1801 gattaacaca ttaaaagaaa tgttcgggat acccgttggg tactccgatc attcattggg
     1861 atatgatgtt ggtatttgtg ctgttgcgat gggtgctaag gtaattgaga aacattttac
     1921 cttggataag gaactacctg gtccagatca taaagcttct ttagatcctc aggaatttgc
     1981 agattttgta aagcatatta gaaacactga aatattttta ggtgatggtg ttaagcgagc
     2041 aatgccttct gaacttagta caaaagaagt atcaagaaaa agcctagtgg tcactgagaa
     2101 tttaaatgtg ggagatattc ttacagagaa atctcttaca ataaaacgtc ctggaaatgg
     2161 aatagctcct aagtttttat atcaatatat tggcaaaaaa gttaaaagac caataagtgc
     2221 agatagtgtt ctgcttgagg aagatgttga atgaaaaaaa tacttatagt aagtggaaca
     2281 cgagctgatt atggaatata taaacctgtt gctgagaatt tactacaaga taattgggat
     2341 gtatcatttg ttgtttctgg aatgcattta tcccataaat atggatttac taaagaattg
     2401 atcttagctg acggatttag aattagtgga gaagttcaaa gtttgttttt agagaacaat
     2461 aagggaaaca tggctcggtc tgtggcatta gagataaatg gtttcacaaa tatatttgaa
     2521 caaactagcc ctgattttgt attattacta ggggatagag gagaaatgtt ggcagctgcg
     2581 atagcttgtt tgtatcttgg aatcaaaaca gcacatattc acggaggcga agtgtctggt
     2641 actgtagatg aatcaataag acatgccata acgaaaatag catctgttca ttttgctgct
     2701 acggaaaaaa gcaaagagcg tttaataaaa atgggggaag attcttggag agt
//
LOCUS       DASJPC010000174         2739 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJPC010000174 DASJPC010000000
VERSION     DASJPC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.182"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="U4A13_002808"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="U4A13_002808"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN1781052.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DASJPC010000175         2702 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJPC010000175 DASJPC010000000
VERSION     DASJPC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.183"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..595
                     /locus_tag="U4A13_002809"
     CDS             <1..595
                     /locus_tag="U4A13_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEN1781053.1"
                     /translation="SFRDIKEKYHLKFEKKKYVLQVFHPVVTELENIEKQILALINSM
                     EQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNS
                     SSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKN
                     VKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            568..1245
                     /locus_tag="U4A13_002810"
     CDS             568..1245
                     /locus_tag="U4A13_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN1781054.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1235..2377
                     /locus_tag="U4A13_002811"
     CDS             1235..2377
                     /locus_tag="U4A13_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN1781055.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2390..>2702
                     /locus_tag="U4A13_002812"
     CDS             2390..>2702
                     /locus_tag="U4A13_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1781056.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IE"
BASE COUNT         1018 a          347 c          521 g          816 t
ORIGIN      
        1 tagttttaga gatataaaag aaaaatatca tctaaaattt gaaaagaaaa aatacgtttt
       61 gcaagtattt catccagttg taaccgaact agaaaatata gaaaaacaaa ttttagcttt
      121 gatcaattca atggaacaac aatctcaacc aattatttgc attttaccta atagtgatgc
      181 tggtagcgaa attatcagaa aaatgtacca gaaatattta aaaacggata ttatttatat
      241 tacgaattta gagccggagg aatacctgac tatattaaaa gagtgcaaat ttatgatcgg
      301 aaattcttct tctgggataa ttgaagctgc atcattcaaa ataccagtaa taaatttagg
      361 aaccagacaa aatggacgag aacaatctaa aaatattata aatgcaaatc taaatgaaaa
      421 tgaaattgaa actgctttga ataaaataca agatcctcag tttataaaaa gcttgaaaaa
      481 tgttaagaac atctacggtg acggcaacag ttctagaaga ataacaaaaa cattgaatca
      541 aatattgaat gatggggaag atattaagtg gattcaaaaa caaatagctt attaaaatat
      601 actagtgatc catacattat tattggggct ggcggacacg caaaagtcat tattgaaacc
      661 ttaagattag aaaataagtt aatatatgga ttttttgatg ataagcaaaa taaaatgcaa
      721 gatctgaaat atcttgggaa aataaacgaa ttgaatagct gggcggatag caaaaaaaaa
      781 atctataaat ttattgtagc tattggtgac tgtgtagtaa gaaaaaagat tataaaaaaa
      841 ttgaatatgc caagtgagca atatggaata gctgttcatc caagtgctat agtggcaaaa
      901 gaaatttcaa ttggttatgg aactgttatt tttgcgaatg ctgtaattaa cccagatgct
      961 gtaattggag agcatgctat aataaacaca ggtagcattg ttgagcatga ttgtagaatt
     1021 aataactatg ttcatttatc tccaggtgta tgcttagcgg gtggagtaca tgtaggtgta
     1081 ggaactcaaa taggaattgg ttctcaatgt atccagatga agaaaattgg atcttggtgt
     1141 atgataggcg cagggagcac tattgttaag gatattccct cacattcctt agcttatggc
     1201 aatccagcta aaattaagaa agaaggaatt aattttgaat tctagaattt tattatcaag
     1261 tcctcatatg ggaggaacag aagaaaaata tgttgctaag gcttttaaag ataattggat
     1321 agcgccatta ggtgaaaatg tggataaatt tgaagaaagt attacaaaat atactggttc
     1381 taagggcgga gcttctgcgt taagttcagg tacagctgcc atacatcttg ccttaagact
     1441 gcttaatgtt ggaaaaaacg atattgtctt tgctccttca tttactttta tagctacagt
     1501 aaatcctatt ttatatcaag gggctgtacc agtgttcatt gactcagaac ctaatacatg
     1561 gaatatgagc cctgttgcac tagagagcgc attagaagaa gctgaaagaa acaaccaatt
     1621 acctaaggca atcatcgtag tgaatttata tggtcaaatg gctgattatg aacgaatttt
     1681 agctattgca aaccaattta atgttccagt gatagaagat gcagcggaat cgttaggttc
     1741 tacttataag ggaatttcgt caggcttatt tgggacaatt gggatctatt catttaacgg
     1801 taataagatc atcacaacct caggtggagg agctctagtg tctagtgata aaaaatatgt
     1861 tgagcattca agatttttag caacacaagc aagagatcaa gcaccgtatt atcaacatag
     1921 cgaaataggt tataattacc gtctaagcaa tatttctgcc ggtatcggac gtggacaaat
     1981 ggaagtattg aatgatagag tagctaaacg aagagaaatt ttcaatcgat ataaggaagc
     2041 tttttctgga attgatagaa ttgaaatgat gccagaagtg gatggcatgg tgccaaatcg
     2101 ttggttgtct actttaacga tcaatgatta taattctatt gatcaagtag ttaacgtaat
     2161 aaataaacta aatgaaaaaa acattgaaac aagagctttg tggaaaccac tacatttgca
     2221 accagtattt caaggggtaa aaatgtatta ccatgataat aatcttagtg tgtcagaaaa
     2281 gttatttaat agcggcttat gcttaccttc tggaagtaat atgacagata gtgaacaaga
     2341 atttgttatt gatgagttta aaaaaatatt caagtaggtg tgatatttta tgaacatagg
     2401 aattattggt ttaggttcca tggggaaaag aagacttaga ctcattcaag aacactttaa
     2461 tgatatttta ctatgtggtg ttgatactaa tgaggcgaga cgaaaggaag ttgaaaagga
     2521 atttggaata ccaacttata atagtctaac agaggcaaaa aatttgtttg aaataaattc
     2581 agtttttgtt tgtacatccc ctataaccca tgaatttatt ataaaagagg ctttaaattt
     2641 agattgtaat atctttacgg aaatcaattt attaaatgaa tattatgatg aaataatcga
     2701 ag
//
LOCUS       DASJPC010000176         2672 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJPC010000176 DASJPC010000000
VERSION     DASJPC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.184"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..322
                     /locus_tag="U4A13_002813"
     CDS             <1..322
                     /locus_tag="U4A13_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1781057.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            736..1554
                     /locus_tag="U4A13_002814"
     CDS             736..1554
                     /locus_tag="U4A13_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN1781058.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1627..1986)
                     /locus_tag="U4A13_002815"
     CDS             complement(1627..1986)
                     /locus_tag="U4A13_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1781059.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          904 t
ORIGIN      
        1 ttataaagca atcattcctg agaacttaga tgaggttaaa ctttatttat gggaagataa
       61 cggagaaatt gtcggtttta gtggtgtaag tgcagaagaa ttagttatgc tatttcttga
      121 tccagatttt attggtaagc gatatggtag tcaaatatta attgagctga ttgaaactga
      181 agatataaaa agaattgatg ttaatagtca aaataaatat gcaaaacatt tttatttaag
      241 ccatggattt gaaattgaat ctgaagaaga cttagatggt tttggtaaac cataccctat
      301 aacgcattta gtaaaaaaat aattcatgaa tggtttatta cctgattgaa catgaataaa
      361 agtaatattt ttattttgat ggcctatttc taggttatga attatccaga tcaaatttgt
      421 gtattacatt cagtaataag gtgcttatct taggatcaac ttatgatact agctgaccaa
      481 ggatgtacca gttatagaaa atcagttaaa gtaaaaatga taaaaatctt atgtcaacac
      541 tgatttaggc tgacctgtgg gttaggagaa agacaggtcg gtagctttta atacaaaaaa
      601 tggcttgtat gtatctagat agttaactga aattagaaat gttcttattt ttaacggagt
      661 aagaaaaata ggctgcttta ggtctgaata ctttttcaat ataatgaaga tatcaaaggc
      721 gacaggagtg tttttatgaa taagaatcct cataatctaa agttggtagc agtagatatg
      781 gatggaacat ttgctcgtac tgattacaca tacgatatcc caaggttcaa ggctgtttta
      841 tcgcgaatga aagctgttga ttgtcaattt gttgttgcaa gtggaaatca atactaccaa
      901 ttgagatcgt tatttccaaa ttattatgat gaattaagtt ttgttgctga aaatggtgct
      961 tttgttaaag ataaaacaga ggtactattt tcagccgata tgcctagaaa agcgatcgag
     1021 gtggtccttg atttatgtaa agactatcct gaagtaaaaa atgtgatgtg tggtctgaat
     1081 agtgcctact gtgaaagagg aaaggtgagt caagcatttt ttgatctaac caatatctat
     1141 taccatagat tagaatgggt agctgatttt gcagaagttc aagataaaat tttaaaattt
     1201 gctccgacta tgcctgttga aaaaaccgaa ttttacgttg aattgttccg tgaaaaatta
     1261 gccgggctat tagtacctac cagtagtggt catggttctg tagacttgat tctttctgag
     1321 tgccataaaa ctttcggctt gaaaatgtta gttgagcgct tggggattaa tcctgaccag
     1381 tgcgttgctt ttggagatgg gggcaatgac attgagatgt tggagtattg tggtctgagt
     1441 tatgaaatgg ataatgcaac ggaagctgtt aagcaagttg ctaagcatca atgtccttca
     1501 aatgacgaag acggtgtttt agtgacgctc gatcgattgt tccctaacac gtaaacacta
     1561 ggtaattcat caaaaaacct cgatataaga aatattcgag gttttaaaac tatatttaga
     1621 acagatttac gaagcgtctg atgagtcaaa atagacttgg acattgctat ctaaaatacc
     1681 actttcattc tttaaacgaa ttgtttgatt tgatggagtt acctctaata ctaaatgccc
     1741 attaataaag gtagtatcgt attcagatgt tttaactgtt gcttcttcgc aaggtattgt
     1801 taaatcatat gctggaatat aatgttgtgt ggaaactaat aaaaaatgat caccaatcat
     1861 acagcatccc tctgctgaag aaaattgatt agagccatca tttaaagtta acaagaatcc
     1921 atcctttaaa cgaggtactt gcttaaatac ttctggttgt acttctaaaa ataaattatc
     1981 gctcattttc acgaccacca tcgacttcca cagttgaaat tgacatttta tttaacacac
     2041 ctattccccc ttaactataa ctcaattcta tataaaaaat attctattct caaataattg
     2101 aacttttcaa atcgccaatt tacttcggct ttatcagagc tcattcatat caagagcgat
     2161 taacgctgta aaaattaaac cgaagatctg ataaattttc tctattttct acaatattta
     2221 aaacgcttcc ttagtctatt atatatctat ttctcttagg agtatgttta gatgtttgaa
     2281 taatagttgc ccaactttaa aggttattct tataatattt tacttcattt tccaatagaa
     2341 aattcaaagg attactggtt tttctaaaat agtttagttt aaatacataa gtatcctccg
     2401 acactgtatt agttcataat catctaaata caaaattttc aaataaaaat atttcaagct
     2461 ctatcgttga cgaatgtaaa cgatacttat aaagtaatag aaattgatta catttgtaaa
     2521 cttaaaatgg ttaatttatc tcacataaaa ctaagaataa aataatgatt aaaagtggtt
     2581 agtttttata cgatttagcc tatcggtaaa aatgaattag agagtgtaaa atattttgtg
     2641 taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DASJPC010000177         2623 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJPC010000177 DASJPC010000000
VERSION     DASJPC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.185"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..417
                     /locus_tag="U4A13_002816"
     CDS             <1..417
                     /locus_tag="U4A13_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN1781060.1"
                     /translation="ENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDE
                     FPQFYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFE
                     EVVKLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            449..1585
                     /locus_tag="U4A13_002817"
     CDS             449..1585
                     /locus_tag="U4A13_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN1781061.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            1585..>2623
                     /locus_tag="U4A13_002818"
     CDS             1585..>2623
                     /locus_tag="U4A13_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN1781062.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYN"
BASE COUNT          968 a          288 c          561 g          806 t
ORIGIN      
        1 gaaaataaga tgagtaataa tctcatgttt aagatggatt ttgacccaag aatcattggc
       61 aataaaatac ttcctgatgg aacaaagaag acaggaattg gacaatttat tcgatctaca
      121 tcattggatg agtttccaca attttataat gtcctaatgg gaaaaatgag cctggtgggg
      181 tttagacctt gcttgaagtc agaatatgag aagtatgatt ttcatcatcg tgcgagaatt
      241 gcgatgaaac ctgggattac tggaatgtgg caggtgagtg gtagaagtga tatcacagac
      301 ttcgaagagg ttgtaaaact tgatgcagaa tatatcagga attggagtat gggattagat
      361 tttagaattt tgtttaggac agtcaaaact gtgcttcata gaaacggttc catgtaagat
      421 aaagatacta tagaaggagt aaaacgtgat ggcaagaaaa aaattaaaaa tctgtatgct
      481 aggtcataaa agaattccat caagagaagg tggaattgag atagtggtag aagaattggc
      541 aacgcgaatg gttgcacttg ggcatgaagt gacttgctat aatcgaagtg gtcatcatgt
      601 tagcggaaag gaatttgacc agcataaaaa taaagaatat aaaggtgtaa aattaaagac
      661 tatttttaca ttaaatataa aaggaattgc agcaatgagt tcttcagtat ttggtggaat
      721 tagagcagca ttcggaaaat atgatgttgt tcattttcat gcagaaggac catgtgctat
      781 gttgtggttg ccaaaacttt ttgggaaaag atgtgttgca acaattcatg gtctagatca
      841 tcaaagagaa aaatggaaca agcttgctag tacatacatc atgttagggg aaaagtgtgc
      901 tgtaaaattt gcagatgaaa taattgtact tagtgaaagc gtccaaaatt attttgaaga
      961 tatatatgga agaaaaacaa ggtttatacc aaatggcgtt aaaaaaattg aaataaaatc
     1021 agcaggattg attacagaaa agtatggatt aacaaaagat tcttatattt tgtttttggg
     1081 gcgattggtt cccgaaaaag gaattcggta tctaattgag gcatttaaag atgttcagac
     1141 agataaaaaa cttataatag ccggaggaag ctctgataca gatgaatttg caaatgaact
     1201 aaaggaatta gcaaaaggtg atgagcgaat tatatttact ggatttgtac aaggacagga
     1261 attagaagaa ttatatagta atgcgtatat ctatacactt ccaagtgatt tagaaggaat
     1321 gccattaagt ttgttagaag caatgagtta tgggaactgt tgtatagttt caaatatctc
     1381 agagtgtacg gaagtagtag aagacaaggc tatgattttt aagaaatcgg atgtaagtga
     1441 tttaaagata agattagaag aggcatgcaa tcaatcggaa atggttaaag ttttgaagaa
     1501 tcaagctact gagtttattt gtagtaaata taattgggat aaaatcgtac aagaaacatt
     1561 aaatttgtat aggggaaata tataatgatt cgaatattac agtgtgtgaa tgatatgcat
     1621 cgtgcagggt tggagacgat gctaatgaac tattatagga atatagatcg aactaaaatt
     1681 cagtttgatt ttttgacaca tcgtccatat aaaagtgatt atgatgagga aataataagt
     1741 cttggaggaa aggtctatta tgcaccgaga ctttatccac aaaattatcc taaatatttt
     1801 aaatggatgg aacaattttt taaggatcat ccagaatatg agattgttca ttctcatatt
     1861 gatgcgatga gttatttacc tttaaaagct gcaaaaaaag ctgggattcc gatacggata
     1921 gcacatagcc ataatacatc gatagataga gattttaaat atcttttaaa gcaatattat
     1981 cgatgtaagt taacgtctgt tttgacacat gaattttcat gtggaaaagt agcaggtgat
     2041 tttttgtttc gaaatgataa attcactttg attccaaatg cagtggatgc taaaaaattt
     2101 tattatgatg aaagtattag aaatgatgta cgaatgaaat taggaatttc aagtcagacg
     2161 tttgtaatgg gacatattgg aagaatttca tattctaaaa atcatcgttt tttgattgaa
     2221 atttttaaag aatttcacgc tattaagaat aattccgtat tattaattat tggaactggt
     2281 gatatggaag aagaaataaa aaattatgca aagaaaagtg gcatagatga tgatattaaa
     2341 tttttaggaa atagaaatga tatagaaaaa ttttatcaag catttgacgt attaatgttg
     2401 ccatcattat ttgaaggggt tccgttagtg ggtattgaag ctcaatttgc agatttaccg
     2461 tgtttctttt cggagaaagt tcctacagaa gttgctttca gtgataagac caatttcata
     2521 agtttaaatc aatcggcaaa agaatgggcg agagagattg caaatgtaga tatctctcat
     2581 cgagattcag aacgttcgat tttaataaag gcagattata ata
//
LOCUS       DASJPC010000178         2621 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJPC010000178 DASJPC010000000
VERSION     DASJPC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.186"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1023
                     /locus_tag="U4A13_002819"
     CDS             <1..1023
                     /locus_tag="U4A13_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN1781063.1"
                     /translation="NYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTD
                     LNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVT
                     VTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNK
                     KGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLP
                     DGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNG
                     SKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKN
                     RKKENE"
     gene            1157..2500
                     /locus_tag="U4A13_002820"
     CDS             1157..2500
                     /locus_tag="U4A13_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN1781064.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1050 a          329 c          488 g          754 t
ORIGIN      
        1 aattatgaag gtgtcattga agtaacgtat ccagacggta cgaaagatac ggtgaaagtt
       61 ccagtagaag tgacagacaa tcgttctaat gcagataaat acaatcctga aggacaaaaa
      121 gtgactacag acttaaataa agagccagat gcatctgagg gaattaagaa caaagaagat
      181 ttaccaaaag gaactactta tgcttggaaa gagaaagtag atgttagcac agctggaaat
      241 aaaaaaggta ctattgtagt gacatatcca gatggatcat ctgatgaagt tgaagtagac
      301 gttacagtga cagatactcg ttccgatgca gataaatata atcctgaagg acaaaaagtg
      361 actacagact taaataaaga gccagatgca tctgagggaa ttaagaacaa agaagatcta
      421 ccaaaaggaa ctacttatac ttggaaagaa aaagtagatg ttagtacagc tggaaacaaa
      481 aaaggtactg tagtagtaac atatccagat ggatctaaag aagaagtaga agttactatt
      541 tctgtagtag ataaaaaagc accgaataaa cctcaagttg atcctattac agagggtgac
      601 caaattgtta ctggtaaaac tgaaccaaat gcagaggtga cagttacatt accagacgga
      661 agtcaacacc atgggacagc tgataaaaat ggtaatttta cagttaaagt tcctaaatta
      721 gaggcaggga caaaagtgat agtaactgca actgatgaat ctggtaatac tagtgaacct
      781 actaatgtag ttgtttcttc caacaaaaaa gacagtggaa aaaacggtag taaaggtagt
      841 aagactgaca atcaaggtag caacagcaat caagacaaaa atagaggcaa aagccagtcc
      901 tcaaaagttt ttcctaagac gggagagagt gacagtaata tctttactat tagcggtggt
      961 ttaatactgt taggaacgtt aggattatta gggtataaaa atcgaaaaaa agagaatgaa
     1021 tagaaaatca atttcttaag tcttctctga gaagaggaag aatctttttc ttcctctttt
     1081 tgctttttta tgatgggaaa taagtgttag ttaaagatga atatactttt tatagaaagg
     1141 aaaagaatgt aattaagtgg aaaaaatagt aattattggt tcaaaccatt cagggctagc
     1201 cgcagctcga tttctaaact taagcgattc aaattatgaa gttattctaa tcgataaaaa
     1261 atcagaaatt agttacttgg aatgtgggac atcgttattt ataaaaggag aagtaagtaa
     1321 tttagaagat cttttttatc aaaatagaat gacactaaat tctgagataa aaaagttatt
     1381 tctagagaca gaagttgaca aaattgatat tcaaaataaa aagattagtt gtgtaaaaaa
     1441 agatggtaca agattctctc aagaatatga caaagttata ttagctacag gttcaaagca
     1501 aattatgcta gatataccgg ggaataattt agctaatatt ttttcgataa agaattttcc
     1561 aaatgcaaaa gagattttta aagctttgga tgatagtact attaaaaaag taggaattat
     1621 tggagcagga tatattggag ttgaaattgc tgaagccata agaaaaagag ggaaagaagt
     1681 atatcttttc gatgttgcag atagagtgtt gtcgacttat tacgaccgta gtttttcaga
     1741 taaagttgaa gaaatactat caaaaaatgg aatacatctt tgtctatcag aaagtcctaa
     1801 aaaatattta ggaagaaaaa agattgaaaa aatagtcact acaagtggta cccaatactc
     1861 tatggatatg gtcgtacaag ctataggatt tctatctaat agtccaattg gggaaaagga
     1921 actcttgaga gatgttagtg gagcatatct aactaatgaa taccagcaaa caaatataaa
     1981 agatatatat gctattgggg attgtgctac aacttttttt acgtccatta atagaagtag
     2041 tgttgatttt tctatttcta atgctctaag aacagcttat attgcaactc aacatattaa
     2101 caatcaagat tttacacctt ctggttccca atttacaaat ggcttttcta tatttaatta
     2161 caatttttac gcagcaggtt tgaatttaaa aacagcacga ttattaagtg agtctataga
     2221 ttatatagaa atagaagatt tcattagacc tgcttttttg agaaaaaata gtcgtgtaaa
     2281 attaagaatt agctttgaaa aaaatacaaa gagaatagta ggtgtacaac tttgtagtca
     2341 agaagattta tctggactat tatcaatgtt ttctcttgct atagaagaac aaattacatt
     2401 agacaaattg aaaatgttag attatttatt tcatcctagt tttagccaac cctataattt
     2461 tattttaaag gcagtaacag gattaaacat tgaaacataa tcgttactaa atgaaatcaa
     2521 gaaatatact gtattactca attcattgaa aatatttatc ataattttta atagaaaaca
     2581 ctattaaaga gaaaaaagag aggtaaatca aatgaaaaaa t
//
LOCUS       DASJPC010000179         2469 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJPC010000179 DASJPC010000000
VERSION     DASJPC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.187"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1201
                     /locus_tag="U4A13_002821"
     CDS             <1..1201
                     /locus_tag="U4A13_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN1781065.1"
                     /translation="PEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLTSSQQKG
                     IVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGIISYFYP
                     VFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEEEQQKIG
                     TFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            1214..>2469
                     /locus_tag="U4A13_002822"
     CDS             1214..>2469
                     /locus_tag="U4A13_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN1781066.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          857 a          410 c          511 g          691 t
ORIGIN      
        1 accagaaatc cgtttcccag gatttacaga tgattgggaa cagaggaagt tgggggactt
       61 cgttgtagat tatgttgaaa agacaagtgt acaaaatcaa tttcctatgc taacttcatc
      121 tcaacaaaaa gggatagttt tgcaagaaga ctattttgca aatcggcaag taactacaga
      181 aaataatatt ggttactttg tactacctag aggatatttc acgttccgta gtcgaagtga
      241 taatgatgtg tttgtgttta accgtaatga cattattgat agagggatta taagctattt
      301 ttatcctgta tttactttaa aatctgctga ttccgacttc ttcttaagac gtattaacaa
      361 tggaatccaa cgacaattat ctattcaagc agagggaaca ggtcaacatg ttctgtctct
      421 aaaaaagttt aaaaatatag tagcaatgtt tcctagtgag gaagaacaac aaaaaatcgg
      481 caccttcttc aaacaacttg acgacacgat cgctcttcat cagcgtaagt tagatttgct
      541 gaaggaaacg aaaaaaggat tcttacaaaa aatgttccct gaaaacggag caaaagtgcc
      601 agaaatccgg tttcctggtt ttacggaaga ttgggaagag cgtaagttgg gagaattagg
      661 taaaactcaa tcgggaattg gatttcctga tgccgagcaa ggtggttctg aaggtactcc
      721 attttttaaa gtttccgaca tgaataacat tggtaatgag tatgaaatga gaaatgcaaa
      781 tcattatgta tctaatgagc aaattgagcg taaaaaatgg aagccaataa aagatgtgcc
      841 agcgattata tttgctaaag ttggcgcagc aataatgctt aatagaaaac gtttggtcac
      901 aagccctttc ttaatagata acaataccat ggcttattta tttaataata cttgggatat
      961 ctattttggt aaaatattgt ttgaaactat aaatttgcca agatactccc aagtaggggc
     1021 attgccttct tataacagtt ctgatattga gaatatcagt gttaaggttc ctgtaaagga
     1081 tgaacaacaa aaaatcggta ctttcttcaa acagctagat gatacgatcg ctcttcatca
     1141 acgtaagtta gatttactaa aagaaacgaa aaaaagcttc ttacaaaaga tgtttgtcta
     1201 ggagggaaga agaatgaaaa ttagtcaccg tgaggaagca gaagtcgaag aacagttgat
     1261 tcgtgtactt ggagaagggc ataatcagtg gacgtatcgt ccggatctaa aatcagaaga
     1321 agacctttgg gtcaatttac gccaaaaaat tatctcaaat aatcaagcag aactgaatga
     1381 ttttcctttg accgataaag aatttgaaac gatcaaaacg gaattactgc tgcgaacgaa
     1441 aaccccattt gatgctgcga aatggttaaa aggtgaaaat gggatggctc ggatcacgat
     1501 tgaacgggaa gattcgcaat tgggttctgt ctcacttatc ctttattcca atcaagacat
     1561 tggtggagga atctctactt atgaagtggt ccatcaaatt gcaaaacgtg gaagtaacat
     1621 agaagcacgg gatcgacgtt ttgacgtcac cttattaatc aatggattac cgattgtcca
     1681 aatcgagctg aaacaagtaa ctgcaaaaga tggtgtttat caggcattta accaaatcaa
     1741 aaaatatgca gaagaaggta tgtttcggaa caatatcttt tcgaccttac agttgtttgt
     1801 ggtttccaat gaacaaacca ctcggtattt tgcaaatgcc ttaccgaaag acttgcaccc
     1861 taagttttta tttagttggc gaacgaaaga caatgaaaaa gtagaaaact tatatgagtt
     1921 ttgtaaacaa gtgttaaata tcccggatgc tcatcgtttg attgcggatt atacaatcgt
     1981 cagtgaagat caagacaata aaaccctcat ggtgttacat ccttatcaag tacatgcgat
     2041 tcaagccttg tttattgccg caaataaaca tcaatcaggg tatgtgtggc atgcgactgg
     2101 ttcaggaaag acgttgacta gttttgtatc tactaagctt ttagcacgaa aatcggggat
     2161 cgatcgaacg atcatgttag tggaccgcaa agatttagat aatcaaacca ccacagagtt
     2221 tacaaaattt gcttcggaat ttaatactgg gatttcttcc gggaacgcca aagcgaatag
     2281 cttgatcgtt ggaacgggaa gtgccaaaga attaagtgaa accctgttgg cagatgccaa
     2341 tgcaaatgtt gtaatcatta caacccgtca aaaactagat gcggcgttga aatatgcaaa
     2401 gaaacaagaa gagaaaaaag gaaccaatcg cttccaaaaa ttaatgggcc aacatatcgt
     2461 gtttgtagt
//
LOCUS       DASJPC010000180         2463 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJPC010000180 DASJPC010000000
VERSION     DASJPC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.188"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            7..624
                     /locus_tag="U4A13_002823"
     CDS             7..624
                     /locus_tag="U4A13_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781067.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..>2463
                     /locus_tag="U4A13_002824"
     CDS             1644..>2463
                     /locus_tag="U4A13_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1781068.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          901 a          326 c          396 g          840 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tca
//
LOCUS       DASJPC010000181         2411 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJPC010000181 DASJPC010000000
VERSION     DASJPC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.189"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            122..514
                     /locus_tag="U4A13_002825"
     CDS             122..514
                     /locus_tag="U4A13_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781069.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(687..869)
                     /locus_tag="U4A13_002826"
                     /pseudo
     CDS             complement(687..869)
                     /locus_tag="U4A13_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            856..1053
                     /locus_tag="U4A13_002827"
     CDS             856..1053
                     /locus_tag="U4A13_002827"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781070.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1096..1671)
                     /locus_tag="U4A13_002828"
     CDS             complement(1096..1671)
                     /locus_tag="U4A13_002828"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN1781071.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1826..2352)
                     /locus_tag="U4A13_002829"
                     /pseudo
     CDS             complement(1826..2352)
                     /locus_tag="U4A13_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          764 a          413 c          377 g          857 t
ORIGIN      
        1 ttttttcttt taagtaaaaa atataggtga ttttttttga cttcatattt ataataaaga
       61 cacgtaaatc aattattttt cattttactt taactaataa tgtttatttg gagggattat
      121 tatgttagat gatagattga cgtgttctaa ttgtggtaca ttattttact atgaggaagt
      181 tagaaatatt gtaaaacata gaaaaaaaga aatgcctata cgttgtccaa attgcaatga
      241 agttattaag cgaaagttgt ctcatggtta ttttgtcagc tacgaactat atgaagaaag
      301 taaaataaat ctgaaaaaaa aaccaaaata ctataaaaat ttgaaaactg gagctattat
      361 aactgaaaaa gaatttaaaa atttagtgac tgaagaagaa agcaggtttt ataatttgtc
      421 tggtaatatt tgccctggat ttatggatca gattattgaa aatgagtcaa ttgaatcaat
      481 tgaaaactat gttcctgttg acttaccaaa atagaaaaaa taatcctgtt tttacagtga
      541 attattttaa ttttgatttt caataaaaat atattacaat aataatcgta atagctaatg
      601 aaacaatttc tcaatctttt taccactcca aacagggagc gtcaataatt ttgtgtaaat
      661 aactcgtcct tctgcaaaat aatgggttac tcagtaaaca ttgaagctaa tgtatccgtc
      721 acttgttgga agcctttatg gcttctattt aagaattttt gattgtatgt atcaaagatg
      781 gatactagga aacgctctag tgattcttca ttttgaaact gctcttttct gcggctgtat
      841 ttcttgattt gtttattgaa agattcgatt gtagaaccag aaggaatgga gaacactttt
      901 gaactttaca acaaaactac tgttgataaa ttaattccaa ttttagttcc aactgattta
      961 aatggtcatg atgttttact tcgttttaac ggatttttag gtgaggatta tagataccta
     1021 aaaatttctg atcctcaacc tttaacagaa taataaaaaa cgtgtaatca attatttgat
     1081 tacacgtttt ttatttcaat cttcgttgta gtagtggttg cgacataccg aaaggtaatc
     1141 ttcattacct cctactacaa tctgttcacc tgaataaact ggaataccat caacaatacg
     1201 agcattcatt acagcctttt tattgcaaaa atggcaaata gttttaattt cctcaatttt
     1261 atcagcaaat gtcaataatg cttcggatcc cttgaagaga ttattttgaa aatcattctt
     1321 caatccatag caaattactg gtacttcctc attcattacg ataagtgata gatcaataat
     1381 attctctcta cttaaaaatt gagactcatc gaccaaaata caatcaactt ttccgtactg
     1441 atcaataatc tccactaatt ctccgtacac atccatttca gaagtaattg caatagctgg
     1501 tttcgaaaca cctatacgac tctcaacttt tccaaatcct gatcgagtat ctattgcgct
     1561 tgtcattatg ataactctct tcccttgttc ttcataatta tgagccactt tcagtagctc
     1621 aatactcttt ccactattca ttgttccata tctgtaaaat aattgtgcca ttaaatttat
     1681 tcccttcttt tacaattcaa ttattcctta attccatctg cttttatgca gctgctcgtt
     1741 tattccttga tatgtctata acatctacta tccctaaaac caatttataa gccattagag
     1801 atggtattcc tgaacatctc tcatattgtt ttttgagtgg tatatcatct gaaaaactga
     1861 aggaatcatg aaaatagcga catacttatc atttagagtt ctaactttaa atgcatttac
     1921 ttcttcagtc agcactttag tcatgttgga catggtttgg ggcgtgtagt gatgcccata
     1981 cattttctca atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat
     2041 gacggtctcc tctaatgtgt cattcgtccg tctataagca ggaacagtct gttgcttgaa
     2101 ctcgccattg cggtcgcgcg gaatctggag atgaagttcc ccatactcgg tcttgaccgt
     2161 acggtcatag gagccgttac gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc
     2221 gtaatcgagg aaagccgtta actccgtttt gagaagcgta ttgacagctt tttccaaatg
     2281 gacacggaat aatccattca aatcgccttt gttcgctaga gtctttagga tttctgtagt
     2341 aaaatcgttc atatggaagt cctcttttct gtgaatgtgt tgtggtaact ctattctaca
     2401 gaagggactt c
//
LOCUS       DASJPC010000182         2408 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJPC010000182 DASJPC010000000
VERSION     DASJPC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.190"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(243..464)
                     /locus_tag="U4A13_002830"
     CDS             complement(243..464)
                     /locus_tag="U4A13_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781072.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="U4A13_002831"
     CDS             complement(562..807)
                     /locus_tag="U4A13_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781073.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="U4A13_002832"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="U4A13_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="U4A13_002833"
     CDS             complement(2031..>2408)
                     /locus_tag="U4A13_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN1781074.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DASJPC010000183         2407 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJPC010000183 DASJPC010000000
VERSION     DASJPC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.191"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..1150
                     /locus_tag="U4A13_002834"
     CDS             5..1150
                     /locus_tag="U4A13_002834"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEN1781075.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="U4A13_002835"
     CDS             1321..1779
                     /locus_tag="U4A13_002835"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN1781076.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="U4A13_002836"
     CDS             1938..>2407
                     /locus_tag="U4A13_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN1781077.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DASJPC010000184         2401 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJPC010000184 DASJPC010000000
VERSION     DASJPC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.192"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            208..2070
                     /locus_tag="U4A13_002837"
     CDS             208..2070
                     /locus_tag="U4A13_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1781078.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2401
                     /locus_tag="U4A13_002838"
     CDS             2070..>2401
                     /locus_tag="U4A13_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN1781079.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2401 a
//
LOCUS       DASJPC010000185         2378 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJPC010000185 DASJPC010000000
VERSION     DASJPC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.193"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(46..1347)
                     /locus_tag="U4A13_002839"
     CDS             complement(46..1347)
                     /locus_tag="U4A13_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1781080.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            1739..2362
                     /locus_tag="U4A13_002840"
     CDS             1739..2362
                     /locus_tag="U4A13_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1781081.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          759 a          397 c          465 g          757 t
ORIGIN      
        1 gatagagtga gataagaaca tctaaggtag aattcctttt ctactttaga tgttttttga
       61 ttcataggta atcagattat ttagtaagaa aattctggtc ttcattttcc gaaagcttcg
      121 aaagccataa gcgactcgtt tcaatgcttt gatgtgggta tgcagatttt ctattttggc
      181 atttgaataa ggcaattcga gggctaaacg aatgccggat tcatagttta taaaagcttt
      241 ttttatagaa tgcagttctt ttggaacgga attgggcatc gttcttacaa gtttaaagaa
      301 agtgtccgcc tctttgtcgt gaaacgcttg aagaatttct tgatagtaaa aataggcgat
      361 tcgtaattcg tcagagatcg aaagcagacg atccaccact tcactttcgg tgagatacgc
      421 ccaaccaaaa ttgcgccatt tggtccattt gtcataaaca agcttgtcct gtcgtttgat
      481 cagaagccga gacaacgcct ttaattgtcg ataatatttg gcttgtgttg gatcgtttcg
      541 atcaaagctt ttcatgattc gtacgcgaat atcctcaaat ttacgaatga gatgtttgac
      601 aatgtgaaaa cgatccacaa cgagttttgc ctttggaaaa cattccttta tcagagaggc
      661 ataagaaaca ttcatgtccg tcacgatgac ctttaccttc aatcgtgcgc gtcgttcaaa
      721 ttgcataaaa taagaaacga gagccttttt cttccgtgaa ggaagaatat ccagtaattt
      781 tccagtatcg ccatccatac aagaaaagga gtagggaccg acttgatttt tcagtgaacg
      841 aaattcatcc attaataaac aacgcggtaa ccaatttctt tttacttttg tcatggggat
      901 cagagaagcg agtgtccgag aaacggtcga ccaagaaacg ccgcaatgct tagcaattgt
      961 cgtcattgct tgtttttcag ttagctcttc taaaatttta tagcgcactt gtttagcgat
     1021 aaagcagttt ctatcgacaa tgtaggtatt cgccgtaaaa taggtgtggc agccacgaca
     1081 gtggtagcgt tgtttcgcta aagcaagata agtcagttgg ttcgcacatt tattaagttg
     1141 aatgagcgat tttttctttc cgttcttaac gatcatgcct tcatgattca tctgtttaca
     1201 caaaggacag cgacgaattt ctttacttaa cgtcgcagaa acaacaagac tcttgatatt
     1261 cttaatgctc ttcgattcca catttgtgat gaaaatgttt ttatctttga ttccaagtag
     1321 tagtttgata tgatgaagat caggcataca gaacgctcct ttgattgtgt tgtggtgatt
     1381 caattctaac ggagcttctg tatgttttgt tttttcatgc tcaaaaggga ccgatagaat
     1441 gattttctat cagtcctaga aattatagag ccgttagttt taaaataaac taatgtcctt
     1501 atttttgtgt tcacatttct gttacctaag aaatacttaa aggaggtgat gtagccagca
     1561 acgactacaa cttgaacccg ctatctacag tttatttgtt gtttcttttg ggtatactct
     1621 aatggtacaa atccatattt gcttaaagcg ttgatttttc agcggttttt ctttattgca
     1681 aagtgtaccc taagacctgt atactaatag taaatagatt ggataggaat gatggcaaat
     1741 gtatttagct tatttaagag tttcttccga cgatcaaagt ttagctcggc aacataagct
     1801 aattgaaaat tggtcagtca aaaataatat tgctgaagac gatttaaaaa ttttcgagga
     1861 aaaagtctct ggaaaaaata ttgaaaacag accagagctg caagagctta tttctttcat
     1921 acgtgaacga gatacgatta ttattcctag tcttgatcga ttagcaagaa atagtaaaga
     1981 tacaaaagac ttacttcaat tgtttcgtag taaaggtgct tctattgaaa tattagacct
     2041 accgtcgttt aatggggtaa ctgaccctgc attaagagac ttgctaacaa atttagtcat
     2101 agaagtgttt agctatgttg ctgaaaacga acggaaaaaa atcaaagagc gtcaaaaaca
     2161 aggcattgaa attgcaaaga aaaatggtcg ttatactggt aaaaagattc aataccatgc
     2221 caatgcaaca ggaaaaaaca aattgattta cgatgaaatt gttgatggtc tgaagaaaaa
     2281 agaaactatt tctaaaatag caaagaacgc aggagttaca agaaaaaccg tttatcgagt
     2341 aaaaagagat ttaggaatgt aatatgttcc agttagaa
//
LOCUS       DASJPC010000186         2318 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJPC010000186 DASJPC010000000
VERSION     DASJPC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.194"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..573
                     /locus_tag="U4A13_002841"
     CDS             10..573
                     /locus_tag="U4A13_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781082.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(634..999)
                     /locus_tag="U4A13_002842"
     CDS             complement(634..999)
                     /locus_tag="U4A13_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781083.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1075..1320)
                     /locus_tag="U4A13_002843"
     CDS             complement(1075..1320)
                     /locus_tag="U4A13_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1781084.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1390..1644)
                     /locus_tag="U4A13_002844"
     CDS             complement(1390..1644)
                     /locus_tag="U4A13_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781085.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1808..2131)
                     /locus_tag="U4A13_002845"
     CDS             complement(1808..2131)
                     /locus_tag="U4A13_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781086.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          882 a          374 c          397 g          665 t
ORIGIN      
        1 aaaataactg tgttatatat taaaaaaggc ataatagtta taagcttttt taaagaatat
       61 ttagaaatta ttaattttcg tctgaaaaca aaaaaagaaa gcgttattat tttagtctat
      121 tttcaggggg ggaaccttat gataaaaaaa ctatttttac tatcaatatt aacgctagca
      181 atggcaggtt gttctgactc tggcttagca actgaactga tcacaaaatc aactgaagcc
      241 acgacagagg agttaaaatg tggaaacgat cacagtgact acagaggctt aaatcgacga
      301 tgcatgggaa tattaggcag aagcaattgt aagcatcgat gtagtaatcg aagaaggtgt
      361 agaagaagca acagaaaaat caaaagaaag atcaaaagaa gcaatctgtg ctgtgcatcg
      421 tatacagacg tcgaagatgc ggtactttta aaccacgtca ggagatgttc atttatgtct
      481 agggaagttg gcgatgaacc aaaactagag tacgctttta gttttgcacg tgaggatgaa
      541 gataacccta aaacttacga cattttagct tgatctaaga tggttaaagc tttttaatga
      601 agtaagagga gatagaaggc ttttctagtt atattaggta agataaattc ctttttgctc
      661 caaatttgct aaacattctg ctagataaac ttcatcgtgt tccggataaa tcggcacgtt
      721 gcgctctaca gtaggtaaat caaaaaatgc ttcgcatgat ttgccacgat acataggatt
      781 tttccactct ggtgctgggc tgtaaccacg tttttccatt tcatccatga caagctgatg
      841 atattggaat aatctataag gagaatagtg aaaaacataa tccacagtag catgtttctt
      901 tccccagcca tttcctctta atgcacagca ttcccgatgt tgtcctaaaa gctgttgacg
      961 aggaagcttt gaaatcaatt tttcatgcca taatcgcata gaaatgtctc ctttatatat
     1021 agattgttaa ccttagacgt taaatgaaaa aaccagcacc aaggctggaa aaaatcactt
     1081 cagtttgctt gctgtttctg agcgaccaat taggtaatca acggaaatat cgtaaaagtc
     1141 agcgatatag attaattcaa tgatgcttgg aatttcttct ccttcttcgt acttacgata
     1201 tttgaataaa ggaatactta acgcgctaga aacagctact tgagataaag atcggccagt
     1261 tcttaagaaa ttcagacgac aacgtaaagt atccagtaaa atagcttctt cgatactcat
     1321 ttaatcacct ttcattatag tagaatagtt ataatgacta tagaaagcta cacatgcaat
     1381 caatagtact caatgttttt tatttaaacg tatcaaataa agaatgacga tcaataaaga
     1441 caaaaccagt cctgataatt cttctataaa aacactatcg ggttcaaagt agttgtaatt
     1501 aaaaactttc cacataaagt agatagccgg aagagtcaaa atagctaaat aaaaccgttc
     1561 tttgttccaa ttcataagat aaacaatact tgcgaccagt gcgaccgcag gcacccaaaa
     1621 caaaactctc accaacgtaa tcatgtaacg gactcctcaa cgattcatat aataaaagat
     1681 aacatgttat tgcatacatc acaaatgaaa tgctaggaaa ataaaagaag aatagatcaa
     1741 tgaaaaatga aaaagcagaa cagttttctg ctgataagca ggaactgttc cgctgaatgt
     1801 gtcttatcta tcgagaaaaa atcaatggat tgatatctaa gtaattcaat acatataaaa
     1861 agaggacgat cgcattcact aaaaaggaaa gaatgatgat cccatagcga atggaaacat
     1921 atttaacaaa tacttttgtt tgaatacggg catatctttt tcctttatag ctgagcatca
     1981 tgacaagaag caaaagggta atcacacctt gaaaaatcat tccaataaac agaattcctg
     2041 aggtttgata atttaattgg agaaaataag ctgctgccgc aactaaatcg aataaaatcg
     2101 caataaatgg caaaataatc actcctaaca ttgagaataa ataaaagaat aacatttttt
     2161 ctaaaataag caaataaaaa ttaaagtaac aaaatagtta tgacacaagt aaaagaaata
     2221 agaataacaa tattatttta cttatatttt gaaacttgtg agagtttgtc attcgtgcta
     2281 tagttgaaaa gtaacaaaaa taaattttat ttatgatg
//
LOCUS       DASJPC010000187         2302 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJPC010000187 DASJPC010000000
VERSION     DASJPC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.195"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(101..2185)
                     /gene="cadA"
                     /locus_tag="U4A13_002846"
     CDS             complement(101..2185)
                     /gene="cadA"
                     /locus_tag="U4A13_002846"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN1781087.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT          667 a          517 c          358 g          760 t
ORIGIN      
        1 tttgaatttt ctgcaaaatt aaaaacatta gttattttat aatcctatct attttcttta
       61 catttttcat taagaaaagg atataaaaaa aagaggacgt tcattcgtcc tcttttttta
      121 catgcaagct tcgcatggca ttcaaaacag ctaatactgt caccccaaca tctgcaaata
      181 ctgcatctcc cattgatgca aaaccaaaag cacctaaggc aagtaccaaa atttttatcc
      241 caatagcaaa gacaatgttt tgtttgacga ttttcaatgt tttctttgct aaatggattg
      301 cttctgcaat cttcgatggt tgatcattca tgatgactac atctgctgct tcaattgcag
      361 catcgcttcc caatccaccc attgcgattc ctacatctgc tcttgctagc accggtgcat
      421 cgttgattcc atctccgaca aatgcggtct ttttattgtt tctttgtaag atttcctcta
      481 accgctgcac tttatcttca ggaagcaatt cgctatatat cttgtccacg cctacttgct
      541 tcccgatatg atcagcaatt tttttcgagt cgcccgtcaa cataactgtg ttcttcacac
      601 cagcctgttt taattgaacc aatgcgtcta cggcatcttc tttcaaggta tctgcaataa
      661 caagataacc actgtaactt tgatcgatcg ctagataaag caatgtaccg atttcttgac
      721 ttaaaggaaa agagatttca aatctttcca tcaatttgtg attacctgct aatacttgtt
      781 tgccatcaac ttcagctaag atcccttcac ctgcaaattc ctggatatta gttgttggta
      841 aaagcggacc tgcgtagcct tctaaaacag attgagcaat cggatgtgta gaaaactgtt
      901 ctgcactcgc aatatattgc atgaatttct ttggatcgat ttttgtatcg atcgtctgga
      961 tcgaaaagtc acctttcgtt aatgtgccgg ttttatcgaa taccactgtc tcggtttgcg
     1021 caagcaactc caagtaattt gaacctttta ctaatacacc gattttactt gcgcctccga
     1081 taccgccaaa gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta
     1141 aaaatgtcaa tgctcgatag atccattcag aaaacgccgc tccagaaaca acaagtggag
     1201 gtaaaacagc taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt
     1261 tagtaatgaa attttcagct ggtgcttttt tactgctggc attttctact aattctaata
     1321 atttgctgat agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt
     1381 tattgataaa tccgcttaat actgtttcac caacatatac agatttaggg actgattctc
     1441 ctgtaagtgc tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta
     1501 cacgttcacc aggcttcaca agtacaaact gaccgatctg cacgtcttca gggttcacgg
     1561 tcatcaatcc ttccgtcgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc
     1621 gaatcgattt tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa
     1681 atagcatcac tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa
     1741 cagacataag aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct
     1801 tcactacatc atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt
     1861 cattaaaatc aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca
     1921 gcgtatttct ctctttttca tttccatgtt cagatacttt ttccttatct atttctgtta
     1981 ctttgacatc tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca
     2041 aatgatcttc ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct
     2101 tgactccatt gatcttctga actccttttt cgattttcat tgcacagttc gcacaatcta
     2161 aaccttctaa tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaacatct
     2221 cttcataact aattataaat gaatggttat tcatatgtca aggtatccga aagaaaaaat
     2281 gaaaaacatc tgcatcggaa aa
//
LOCUS       DASJPC010000188         2255 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJPC010000188 DASJPC010000000
VERSION     DASJPC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.196"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(284..1624)
                     /gene="glnA"
                     /locus_tag="U4A13_002847"
     CDS             complement(284..1624)
                     /gene="glnA"
                     /locus_tag="U4A13_002847"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN1781088.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(1652..2044)
                     /locus_tag="U4A13_002848"
     CDS             complement(1652..2044)
                     /locus_tag="U4A13_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN1781089.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          642 a          499 c          381 g          733 t
ORIGIN      
        1 cgtctttttc aaaacctctt ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagt
       61 tcagccaatt caaaaacaag aaaagctgtt tttgcttggc tattcttatt acttgttgca
      121 gggcagcttt ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta
      181 gctcaaaaaa cggcaaaata tgaggaacta tttttccgtt agtttctcta atactcaggt
      241 caaaacgctg atttcacagc ctctaattca cggtattcct gttttaataa agttctagat
      301 attgttctct ttcccactcg gaaacagttt gtctgaatgc cgcccattcg agacgttttg
      361 cttcaacaaa atttgagaag atatgttgtc ctaatgcgtc aatcattaca ttatccttac
      421 gcaattcttt gatagcatta tggatagttg aaggtaagtc ctggatatgt gcagcttctc
      481 tttcttcctc atccattaca taaatgttac ggtcaacagc tgctggcggt gtgatctgat
      541 tacggatacc gtctagtcct gcttgcaaca atactgccat tgccaagtat gggtttgcag
      601 atggatcaac tgaccgcaat tccaaacgtg tggataaacc gcgagattct ggtacacgta
      661 ctaatggcga acgattacgg ccactccatg cgacataaac aggcgcttca taacctggaa
      721 cgagacgttt ataagaattg actgttggat tgcaaacggc tgtgtaggca cgagcatgtt
      781 tcagcaaacc acctaagaaa tgataagctg tttcacttag ttccaattca ccgtctttat
      841 catagaaaac atttccttca tcattgaaca aagacatatt gcaatgcata ccagaaccat
      901 tgatcccaaa taacggtttc ggcatgaaag tcgcgtgaag tccatgcttg cgtgcaatcg
      961 ttttgacaac gagcttgaag gtttggatat tatcacaagc atctaccaca tctgcatatt
     1021 taaagtcaat ttcatgctgt cctggcgcta cttcatgatg tgaagcttcc acttcaaagc
     1081 ctagactttc taattccaac acgatgtcac ggcggcaatt ttcacctaaa tctgttggtg
     1141 caaaatcaaa ataaccacct ttatcattca aggtcgttgt gatcccaccg tcttcgtcta
     1201 atttaaataa gaagaattct ggttcaggtc ctaaattgaa agaggtaaaa ccaaggtcgc
     1261 gcatatcttt caatgctcgt ttcaaattcc cgcgaggatc gccggcaaat ggtgtcccat
     1321 ccggattata gatatcacaa attaaacgag caacttttcc atgagcactt tcccacggga
     1381 agatcatcca agtggagaga tctggataca aatacatatc gctttcttca attcgaacaa
     1441 atccttcaat cgaagaaccg tcaaacatca ttttattttc taatactttg tcgatctggc
     1501 tcactggaac ttctacattt ttgatcgtac ctaagatgtc tgtgaacatc aatcgtaaaa
     1561 atcgtacgtt ttcttcctca atgattcgtt tgatttcttc acctgttatc tgtttcttta
     1621 ccatggtatc cctcttcctt ttttaattta ttcagtcaaa gaattgtctt tgcaatcctt
     1681 gcgactgata atgacctgtt ttgcttaacc caccttgcgc caaaatctca tcacgaaaaa
     1741 tttgtcgaac gtcatgatct gtcaacggct tcttgttttt tgcttcttgt tttttcgcct
     1801 gtgccatttc gtatacacgt ttgatcccag ccatattcaa tccctctgaa agatagtctt
     1861 taatctcaag caacatatca atatcattca gcgaatacat acgtcggttt ccttcgcttc
     1921 gttccggatg aactaaatct tgttcttcat aatagcgaat ttgccgagca gacaaatcag
     1981 tcagcttcat aactgtacca attggaaata cggacataga tcggcgcaat tccttttctc
     2041 ccatcagacg tcccctttcg ttttataagt tggctaaagt gtaccaatta aaaaaaatat
     2101 tgtcaactgc atatgttagg ttttctaaca tgagatctct attttttata aaataacaaa
     2161 aaaataagaa cacaaaaaag gtatctagtt gattttcgta ccagatacct tttatttttt
     2221 attatactgt actatccttt attagtgttt ttatt
//
LOCUS       DASJPC010000189         2249 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJPC010000189 DASJPC010000000
VERSION     DASJPC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.197"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(151..2148)
                     /locus_tag="U4A13_002849"
     CDS             complement(151..2148)
                     /locus_tag="U4A13_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN1781090.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          573 a          504 c          411 g          761 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttc
//
LOCUS       DASJPC010000190         2232 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJPC010000190 DASJPC010000000
VERSION     DASJPC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.198"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..543
                     /locus_tag="U4A13_002850"
     CDS             <1..543
                     /locus_tag="U4A13_002850"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1781091.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="U4A13_002851"
     CDS             complement(899..1471)
                     /locus_tag="U4A13_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN1781092.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /locus_tag="U4A13_002852"
     CDS             complement(1487..2092)
                     /locus_tag="U4A13_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN1781093.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc ta
//
LOCUS       DASJPC010000191         2131 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJPC010000191 DASJPC010000000
VERSION     DASJPC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.199"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            32..1054
                     /locus_tag="U4A13_002853"
     CDS             32..1054
                     /locus_tag="U4A13_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEN1781094.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            1076..2131
                     /locus_tag="U4A13_002854"
     CDS             1076..2131
                     /locus_tag="U4A13_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN1781095.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT          864 a          218 c          400 g          649 t
ORIGIN      
        1 tatttgaatt aatacaaaga ggttaaagaa tatgacacag aaacattcaa gtataaaaag
       61 aattatacat cgcatatccg aagaattgaa tgcaacaaga cgaattgcaa agataacatc
      121 ttggaaggaa gcatttgcca cgttacaagc taaaattgat attcaagtca tgaatcgtaa
      181 tggatataat gaaagtaaaa aacagaagga acatttatta aaaaaacatg aggtaatgct
      241 gaagtattat gaaaagactt tttcagattt tttgaaaaca tatgattata gtcaaaatca
      301 aataattaat caatcaaaat caccatattc taactgcatt tgggtttgtt ggtggcaggg
      361 attggatcaa tcgccggaaa ttgtaaaagc ttgtgtaaaa tcaataaaac gaaatgcagg
      421 gaaacatact gttataattt taacagaaga taattataga caatatgttg atataccaaa
      481 atgggtggaa gaaaagaaaa acaaaggaat cattacgaga acaaattatt ctgatttatt
      541 aagattatct ttgctagcaa aacatggagg tttgtggtta gacgccacat tttattgtac
      601 aaaaccaata ccagatgagt attttgatat cgatatttgg tctattagga ggcctgatta
      661 tgctcacgca tccgtagcat caggatattt tgcgggttat tcattagcat gtaatcaaga
      721 gtctagatgg atgtttgcta taattagaga ctttttttta aattattgga aaaataatga
      781 tatgatggtg gattatttga tgatagatta tatgattgtt ttagcacaga gaataaatca
      841 tgaaatagca cagacatttg ataaaatacc tagtaacaat ccattatgtg acgaattgat
      901 taaaaattta aataatatat atgatgaaaa agtatggaac ttattaaaga aagatacttt
      961 actgtttaag ttgacatgga aacaatcttt taaaaagcaa aaagatgata aagttacttt
     1021 ttatgggatg ttggtagaaa acaaattatt ataaatatta gaggtataaa aagttatgaa
     1081 acacaaggta agtgtgatag taccaattta caatgttgaa aaatatttaa gaaaatgtgt
     1141 ggatagttta ttgagtcaga cattaaaaga gatagaaatc gtattagttg atgatggttc
     1201 gccagatgct tcgggggaaa tagcagatga gtatcaaaaa aaatattcta atgtaaaaac
     1261 aatacataga gaaaatgggg ggttaggacc ggcaagaaat acaggaattg agaatgcgac
     1321 aggagaatat gtggcttttc ttgattcaga tgattgggta gaaagtgata tgtatgaaaa
     1381 attatactta gttgcgtcca aagaaaatgc tgatatagta gttagtggac attgtgattt
     1441 tgcgaatgac aaagctatgg tgaaaaagca acatcctttg agtgggaatg tatatgatac
     1501 gcctaatagt attaaaaatg tacgaaaaaa tttatttgga catagtccaa aagatcaaga
     1561 agtagaggcc ttcccgatgt cagtatgtat gtctttgtat aataaaaatc tgctagataa
     1621 atatagacta agatttgaaa aaattttatc tgaagataca atttttaact tgaatgcata
     1681 tgatgtcgca aataaaattg cttttacaga atatacagat tattgttata gaaaagaaga
     1741 acaagagtct attacaaaaa gtttatctcc aaataagcag acggaattta aagaattttt
     1801 aataactcta aaagctatag catcgaaaga agatttagaa tgtcaaatca gagcaaaacg
     1861 tatggcaatt gattattgta ggctttatgt gggcttagtt gatgatgcaa atttgggaat
     1921 taaagagaaa agaaagtata taaaaaaatt tattgaagat gagggattaa ttcaatgttg
     1981 gaagggatat cctacaaata cgctaccatt tcaacaaaaa ttttttcata aaatgcttga
     2041 atgtaagtgt tatacaatat tgttgttgtt aagtagaatt agaaagataa ttaaaaatat
     2101 gaccagaaaa aaattgttta taatattata a
//
LOCUS       DASJPC010000192         2104 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJPC010000192 DASJPC010000000
VERSION     DASJPC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.200"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            169..1539
                     /locus_tag="U4A13_002855"
     CDS             169..1539
                     /locus_tag="U4A13_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEN1781096.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="U4A13_002856"
     CDS             1560..1844
                     /locus_tag="U4A13_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781097.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          731 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cata
//
LOCUS       DASJPC010000193         2065 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJPC010000193 DASJPC010000000
VERSION     DASJPC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.201"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1008
                     /locus_tag="U4A13_002857"
     CDS             <1..1008
                     /locus_tag="U4A13_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781098.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="U4A13_002858"
     CDS             1025..1174
                     /locus_tag="U4A13_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781099.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="U4A13_002859"
     CDS             1171..1617
                     /locus_tag="U4A13_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781100.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="U4A13_002860"
     CDS             1619..1756
                     /locus_tag="U4A13_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1781101.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="U4A13_002861"
     CDS             1794..>2065
                     /locus_tag="U4A13_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781102.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DASJPC010000194         2058 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJPC010000194 DASJPC010000000
VERSION     DASJPC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.202"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..337)
                     /locus_tag="U4A13_002862"
     CDS             complement(<1..337)
                     /locus_tag="U4A13_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1781103.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="U4A13_002863"
     CDS             complement(404..559)
                     /locus_tag="U4A13_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN1781104.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="U4A13_002864"
     CDS             1176..1397
                     /locus_tag="U4A13_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781105.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="U4A13_002865"
     CDS             1398..1655
                     /locus_tag="U4A13_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781106.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="U4A13_002866"
     CDS             complement(1791..>2058)
                     /locus_tag="U4A13_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN1781107.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DASJPC010000195         2003 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJPC010000195 DASJPC010000000
VERSION     DASJPC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.203"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..378
                     /locus_tag="U4A13_002867"
     CDS             <1..378
                     /locus_tag="U4A13_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEN1781108.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U4A13_002868"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U4A13_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN1781109.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U4A13_002869"
     CDS             1859..>2003
                     /locus_tag="U4A13_002869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781110.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASJPC010000196         1992 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJPC010000196 DASJPC010000000
VERSION     DASJPC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.204"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="U4A13_002870"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="U4A13_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN1781111.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="U4A13_002871"
     CDS             852..1040
                     /locus_tag="U4A13_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN1781112.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="U4A13_002872"
     CDS             1053..1610
                     /locus_tag="U4A13_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN1781113.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="U4A13_002873"
     CDS             1642..1884
                     /locus_tag="U4A13_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN1781114.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          308 c          406 g          554 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc ct
//
LOCUS       DASJPC010000197         1967 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJPC010000197 DASJPC010000000
VERSION     DASJPC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.205"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..417)
                     /locus_tag="U4A13_002874"
     CDS             complement(<1..417)
                     /locus_tag="U4A13_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN1781115.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDL"
     gene            complement(414..1067)
                     /locus_tag="U4A13_002875"
     CDS             complement(414..1067)
                     /locus_tag="U4A13_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN1781116.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1355..>1967
                     /locus_tag="U4A13_002876"
     CDS             1355..>1967
                     /locus_tag="U4A13_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781117.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          619 a          350 c          252 g          746 t
ORIGIN      
        1 taaatctttc gcatcttttt gcatatcagc tgtagaaaca aattgtagat ttgttttttt
       61 accatcatta agctttattt ctctttcttc aattatcaaa gaattactat cactttctaa
      121 attaacattt atattatctt tgatttgtat ttcattctta ttattttttt cttttatgaa
      181 tacttttatt tcactactct tggaataaaa ctccaaagtt tgttctatat attttatttc
      241 ttttccattc atattactag aaatttcatt tgccatatta tgaatctttt cttttctcgt
      301 ctccaaatat gttttaggaa agataaaaaa aattaatgaa tgaactaata taattattat
      361 tccaagaaca gaaaatattt gtatgaacat ttttggaaat atctttaatt ttttcatttt
      421 ctctccaatt tatatcctac atttcttata gtagttatac agtctaattg taattttttt
      481 ctaagttctt ttatatatac atctattact cgatcataag gagtttcttc gctatctttc
      541 catacataat caataatttg cattcttgtt aacacttgtc cattattatc caataaacat
      601 tttaatactt ctagttcttt tgcatttaca tctatttctt caccatttat tttagctgta
      661 tagctattaa aattaaccga tagatcctta tattcaaact tctctaaatg tccataattc
      721 ttcttaatta aagaatctat tctagctttt aagaccggca atgaaaatgg cttttctaca
      781 tatccgtcta ctaaattagt aaatgcatca attttatatt cttcatcact aaatgcagtc
      841 aagattagaa ttggtaaatt gcttttcttt ctaatttctt tcaacacttc taaaccattt
      901 ataaaaggta tctgaatgtc taagataacc aaatttatat cgctattaaa ttttgacaag
      961 gcttctcttc catctttagc ttgaataaca gtatatccaa attctgaaag atattcactt
     1021 attccctctc ttatcatatt atcatcttca acagttaata ttttcatata tacctccact
     1081 taagcctata cactagatat tatacagcat attatggctc tataatatct agtgttggtg
     1141 acaccttctg ttgccaatac tttttctttt ttctaataaa tgcaaacaga caccttgaaa
     1201 tcctttagaa taaagggttc tataatattt tgtgttgttg gaaaatcgaa ggcgattttc
     1261 tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta aaatttagtc
     1321 gaccaaaaca tcaccctacc aaaggagtcg ctctatggat aatcatacta gaaaattact
     1381 cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa aagaatataa
     1441 aggcgttcaa gcgcagttta ttaaagggag attggatgac tcaccttccc attgtgaaca
     1501 ctgtggatcg attcgtatta tccgcaacgg ttcctatacg acacgtactc agatattgaa
     1561 agtcaaagaa aagttgacta ttttagaatt aaaacgtacc cgttttctat gctatgattg
     1621 tgggcagacc ttttctgcga aaacggactt agttgatgaa catcatcaac taacaaagga
     1681 actgaagcag gcgattctca tagaactgta cgaaaatcaa tcacgtaaac taatcgctaa
     1741 gaagtacttc gtctcggatg ggacggtgac acggatcttg cgtgaagcaa cgaagcatta
     1801 ccagccacgt atgaacttcc ttccgacggt gttgtgtatg gatgagttca aatcaatgaa
     1861 gtctgtttct ggttccatga gttttatttg tgttgatggg acaacccatc agctatttac
     1921 gatccttgaa gatcgcagac tttacaaact aactcaatac ttcctgc
//
LOCUS       DASJPC010000198         1962 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJPC010000198 DASJPC010000000
VERSION     DASJPC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.206"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..272)
                     /locus_tag="U4A13_002877"
     CDS             complement(<1..272)
                     /locus_tag="U4A13_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781118.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="U4A13_002878"
     CDS             complement(309..446)
                     /locus_tag="U4A13_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN1781119.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="U4A13_002879"
     CDS             complement(448..894)
                     /locus_tag="U4A13_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781120.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="U4A13_002880"
     CDS             complement(891..1040)
                     /locus_tag="U4A13_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781121.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..1386)
                     /locus_tag="U4A13_002881"
                     /pseudo
     CDS             complement(1054..1386)
                     /locus_tag="U4A13_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DASJPC010000199         1936 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJPC010000199 DASJPC010000000
VERSION     DASJPC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.207"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            84..1550
                     /locus_tag="U4A13_002882"
     CDS             84..1550
                     /locus_tag="U4A13_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN1781122.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
BASE COUNT          597 a          331 c          396 g          612 t
ORIGIN      
        1 aaataacact gtcttgtaag tttgttaaaa attgaatatg attacatctg gataacaaat
       61 ttttatcata aaggagagaa cgaatgaaca acaaacaatt acgctggttt actgttgcgt
      121 taattgcatt taacatggtt tggggcatgg ggaatgtagt taataactat gctcagcaag
      181 gagtatcagt tgtcacctct tggctattga ttttggccat ttattttatt ccatacgcat
      241 tgatcgttgg acaattagga tcagccttca aggatagtaa aggaggcgtc agttcatggg
      301 tcgaaaatac gacatctaat aaacgactgg catactatgc cgcttggact tactgggtcg
      361 tccatattcc ttatttggca caaaagcctc aagcaattct gattgctggt ggttgggcag
      421 tccaaggaaa tggaaatatc gtaaatacga tgtcagtaca aatggtagca gttatttcac
      481 ttattatatt tcttgccttt ttatatttat caacaaaagg actttctact ttgaaagtga
      541 ttggcggact ggccggaaca gcgatgttcg tcatgtcgat cttatttatt cttttagctg
      601 taacggcacc gttcatcaat ccaacgatgg aatttgccac accacatatg aatcaagtga
      661 aaacatatat tccaacattt gatttcagtt atttcacaac cgtttcgatg ctagtatttg
      721 cagttggagg ggcggaaaaa atatcccctt atgtcaactc tacaagaaat cctgcaaagg
      781 aatttcctaa agggatgatc ttcttagcag caatggtagg gatttgtgct gtattaggct
      841 cagtcgcaat gggtatgctg tttgctagtg ataatattcc taaagacttg atggcaaatg
      901 gggcatacag tgcttttcaa attttaggta attactatgg tatcggtaac tcattgatga
      961 tcatttacgc attgacaaac atgattggac aaatctccgc attagccttt tcaattgatg
     1021 caccactaaa aatcttgtta gcagatgcgg atccagaatt tgtaccaaac tggctacgta
     1081 aacgtaatcg taaaggtaca ttggtgaatg gttatcttct gacaggtata ctagtaggaa
     1141 tcattatttt gttgccgatt tttggtatca aagatatgaa tgaattagta aaatggttga
     1201 cgaacttgaa ctcagttgtc atgccaatgc gttacctatg ggtgttcttg gcatatatga
     1261 tgttgaataa agcttataag aaattcaatt ctgaatataa atttgttaaa aatccaaaaa
     1321 tcggttttat ctttggtgct tggtgctttg ccttcactgc gtttgcatgt atcctaggaa
     1381 tggtgccaaa agtagattat actgctgatc ctaaagcatg gtggttccaa ttatttacga
     1441 atatcatcac accaatcgtc ttgatcttat taggtatgat cttgccagca attgctcgtc
     1501 gagacaaaaa caaaaaaatc actgaaccag aaacagtggt tcagccttaa aatagaaata
     1561 cttgttacaa gaggttgtga cagaagtggt cagcttcaag aaataagaag gaatttacga
     1621 aaattgcttc tcaaattttt gtgaagtttc ggcttgtttt tcgaagaagc tacttctgga
     1681 acaccgtgaa tcagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa
     1741 caaaaatagc ccctcatatt tttcgtttgg ctgggcttat taccgcagat gcaagctttg
     1801 gaacaccgtt taaggaagag gttgtaaaat cagcgatttg acctgagtat tagagaaaca
     1861 aacggaaaaa tagctcctca tattttgccg ctttttgagc taatgctgaa ggtcagctga
     1921 ttgaacacca tttatt
//
LOCUS       DASJPC010000200         1799 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJPC010000200 DASJPC010000000
VERSION     DASJPC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.208"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="U4A13_002883"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="U4A13_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN1781123.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DASJPC010000201         1749 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.210, whole genome shotgun sequence.
ACCESSION   DASJPC010000201 DASJPC010000000
VERSION     DASJPC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.210"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(170..1606)
                     /locus_tag="U4A13_002884"
     CDS             complement(170..1606)
                     /locus_tag="U4A13_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN1781124.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT          478 a          385 c          291 g          595 t
ORIGIN      
        1 caagtttcat tatacaaaaa aaagtaaggg aactaaagtt ttgtttgaaa gaactgacgc
       61 tttctttcat ttttcagctt attttacagt ttttcttaat ttctaaatga ttttatcaag
      121 aaaaggcacg gccatatcca gccgtacctt cttgttttaa gtatgtgaac taaaggttct
      181 ctccattttc ttcaatcagt ttttggtacc agtaataact gtctttctta tatcgttcca
      241 aagatccttc ttgtgtttcg tcttgatcaa catatacaaa tccatatcgc ttttgataac
      301 cgttcagcca gctaagcaaa tctgtataag accaggtgca atagcctagg acttctgctc
      361 catccgaaat tgcttctttg atggctttga cgtggctttg taagtaattg attcgatagt
      421 cgtcatggat ttgtttatct tctgtcaact tgtcatactc tcctaagcca ttttccgtaa
      481 tcagtaccgg catgcggtaa cggcttgtga tacgacgcag tccgatcctt aatccttctg
      541 ggtcaatctc ccagtcccaa tttgttcgtt cgacaaatgg attctcaact tttttataca
      601 ttccaggaac tccagtttct tctgatgaac ctttttttcc tgtcgtattc atttttccag
      661 caccaactcc gtttaatgga ttgaatgcat tcgtcgccgt ttgatagtaa ttgatcccaa
      721 gaaaatctgg tttagcagat tctagcaatt ctgcatcgcc agcttcaatc gttggtgccc
      781 atcctttttc ttctaagaac ctcatggcag caatcggata ttttccccac atatagacat
      841 ccatccaata attggccatc aggtcttctg cattttcagc agccagaaca ttttcaggat
      901 tactgtcaat tggataattt ggcgtatacg caaagcttgg tccgattttt ccaggcattt
      961 ctaactcgtg aaatttattg atgacagatg catttgctaa attagcaata tggttaactt
     1021 catacattcg tttcgggtct gtcacacctg gcggatgtgc tgctaacaaa taacctaatg
     1081 aagtgaagat attttgttca ttcaaactaa tccagtaatg gactttcccg cggaatgcct
     1141 caaataatgt ttctgcataa tgagtgaaat cttcaatgat ctgacgtgat tcccaaccat
     1201 tatattcatc ttgcaatgct tgtggaagat cccaatgata taaagtcaac ataggttcaa
     1261 tatcgttttc aatcaattcg tcgatcaatc tctcataaaa agcgagccca gcttgattga
     1321 cttctcctcg tccattagga aaaatccgtg tccaagctac agagaagcgg taagctttca
     1381 gaccttgttc tttcattaaa gcaatatctt ctttataacg atgatagtgg tctactgcca
     1441 aatcaccgtt cgttccttta aatgtctttc caggtatccg tacaaatcga tcccagactg
     1501 attctccttt cccatcttct tgccaagctc cttctacttg atacgctgca gaagcagagc
     1561 cccacagaaa atcttttgga aatgaggtaa gttctttgtg ttccattcat ctcttccttt
     1621 cctataacct tcaatcgttc ctcttttaga ataacacgaa accccttcca caacaaaata
     1681 aaattatgta aaggaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg
     1741 tgaaaatgg
//
LOCUS       DASJPC010000202         1729 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJPC010000202 DASJPC010000000
VERSION     DASJPC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.211"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            116..1351
                     /locus_tag="U4A13_002885"
     CDS             116..1351
                     /locus_tag="U4A13_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781125.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            1492..>1729
                     /locus_tag="U4A13_002886"
     CDS             1492..>1729
                     /locus_tag="U4A13_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781126.1"
                     /translation="MKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESK
                     SFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKS"
BASE COUNT          597 a          175 c          297 g          660 t
ORIGIN      
        1 cttgtatgga aaaatcagta caataccatt atttaatgtt cttcacttaa agcgcaaaaa
       61 atatcagttt attaaactcg tatatagaac aaatatatgt agacggggcg aaacgatgag
      121 tgtaaaaaaa atgctattta tggtaagtac aataattgta ttaacatccc gattttgggg
      181 aattaatact agtttatcta tgcgatattt tattatggca atatgggatt tatattttat
      241 gttaagctat ttttcttatc gtgagaaaac atataaaaaa tacgttatca agaataactt
      301 ttggttgact ataatgccgt ttgtgatatt ttctatctat actctaataa tatggggaat
      361 tggttcaaat gcagaatttg aaaattttat aaagttatgt tccacgttgc tttatttaat
      421 tcttgcatat ggttacgcat gtacagcatt ttacttattt aaggcagaag gaattgattt
      481 tattttttgg tcaggtgtta tttcatatat gagtggttca gttttttatt taattgtatc
      541 ttatggttta aatggtttaa tttcttatat aaaaagtctt ataacaggtg aaacaaatac
      601 tgcaaatttt gctatggagg tacatgattt aacttttgcg atgggggttt ttttccttta
      661 ttacgtattt tttgaaaata aaaatacaaa aaaacataag ataaaaatta tcgtatcttt
      721 aattttaatt ttcatgggat tgaagagaat tgaactgtta gctttaagtg gtgcaattct
      781 agtgtattat gttttattaa agtggggaaa aaagattcaa atccgtgcat tagtgtgtac
      841 tatttgtgca actgttattt cgttagtgtt tatttacatt atagatacag gaattttggc
      901 attgcttatg tcaaatttgg gcattacaga tgacggtgcg aggctttcat tttatctata
      961 ttcatctaaa ttttatgaac ttggtttagg atatgtagga acaggatgga catggttttc
     1021 aaaatacttt tttaatcttt atttatcgca ctttaggtta aatggtggaa gaattgcagc
     1081 aagcctacac agtgatatat taactattta tatagaagtt ggatgtatag tttttattat
     1141 atggatgttt tggttatttt ttgtgaaacc gataagacta actaaaaaat ttggattgaa
     1201 aacaggggag tgtgtattat tattaactat atatatgttt atattatatt tgacggataa
     1261 tacgctcact tatccagaca ctcaaatgat ttttctctta gtacctatga tagtttcgtg
     1321 ggttgataat caacaacgaa tatcggaata gatagctaaa gtatatttta attatgaatt
     1381 tataattagt tgtaaaagtt aatatttaac ggacaggaag tagggtgaaa aaatttgaaa
     1441 aaaattttag tgttagcaaa tacatattat caaataatag tagcaataca aatgaaaaat
     1501 acaatttttt ccgatgagta tgttgaaatt attatttctg atcattctaa aaacactaaa
     1561 gaaattgcga aaaaaataga agaaaaaaaa gtgtttgatg gctgtcatta tttagaaagc
     1621 aaatcttttg atgataatat tgggtatttt caaaagatta aggactgtct tcagatttct
     1681 tgtggtaaat caaatcgata tgcctattat ttgaaaaact gtaaaagtg
//
LOCUS       DASJPC010000203         1684 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJPC010000203 DASJPC010000000
VERSION     DASJPC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.212"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..552
                     /locus_tag="U4A13_002887"
     CDS             <1..552
                     /locus_tag="U4A13_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781127.1"
                     /translation="IKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGY
                     GIDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSST
                     LLISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRL
                     SEEKILLPENWAELRKLVEYGII"
     gene            539..>1684
                     /locus_tag="U4A13_002888"
     CDS             539..>1684
                     /locus_tag="U4A13_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN1781128.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPR"
BASE COUNT          557 a          218 c          269 g          640 t
ORIGIN      
        1 ataaaaaaaa ttcccaaaat agatgaaaac agtaaattga tgaaaaacat acaatcaatc
       61 tttgggatag aagaagtttt tgtcggagat aaatgtatct tttttgatca acctttatta
      121 tctgatggtt atggcataga tgaaacagat attttcgcta aagtaaatag tatattttct
      181 ggaaaaaaag aaactttaat taagcttcat cctagagcaa ggacaaatcg ttatggtgaa
      241 aaaaacatat tgaattccag tttaccgttt gaagtcttgc tacttgaaaa tgacttctct
      301 tcaacacttt tgattagtcc tatatctaca atttcttttt caccatctat gatgtttgaa
      361 aagacgatca actctattat gttggctaaa ttaattttaa atgaatatac agcattatct
      421 gaagataaaa aagaaacatt gacaaatatt gttcttttct gtgaacatta taaccgtcta
      481 tcagaagaaa aaattttact cccagaaaac tgggcagaat taagaaagtt ggtagagtat
      541 gggattattt aacacacttt ccaagtcaaa attagttaag tccagtttct ggtatactat
      601 agggaatttt tttattaagg gaatttcatt cataactatt cctatttttg taaatattac
      661 tactcaagca gaatatggtt tgattaataa tttcacttca tttgtatcaa ttttttcgat
      721 tcttgttggg ctaagcctaa attcggcaat taataatgca agttttgact ataaagatga
      781 cataaatgaa tttatgtcat cggtactttc tctagctaca ctttcgttaa ttgtgttctt
      841 attaataggg aacatatatt ttttgttttc aaatgctttt tttgatatga accaacttgt
      901 atttaatttg atgttattac aaagttttgc tagttttgta attagttata tccagtctta
      961 ttttacgatt aactttaacc attttaaata tttgatttta tcttttatat ctactttttt
     1021 gaatattgca atctctgtag ctttgatgca gacattatat tttgatgata aatatgttgg
     1081 cagagtagtc ggcggaacac tgtcatttgt gttgataggt ttagtaattt atttaagaat
     1141 aatgataaag ggtgctcatt tttttgataa aaaacagtgg agatacgctt taaaaatctc
     1201 tttgccgcta atcccacatt ccttagcaaa tatagtttta gcacaattcg atagaattat
     1261 gattaattct tatttaggtc ctgctgcggg gggaatatat tcctacatta gtaacatagg
     1321 tattattcta agcgtaatat ggatgtctac taataacgcc tgggttccat ggttctatgg
     1381 tgagatgagt aaaaataatg aaaaaaaagt gaagaaagtt agtaactatt atttagtagt
     1441 ctttactttg attacgatga ttgtaatggt agtttcgatt gatttagcaa aatttatggc
     1501 tccgaaagac tattattctg ggttatcttt agtggttccg ataaccttag gctatttctt
     1561 tcagttttta tatagtttac cagttaatgc tgaattttat gagaaaaaaa catcgtttat
     1621 tgcgattggt acagtagcaa gtgctatagt taatatacta ttaaacatga ttttcatacc
     1681 tagg
//
LOCUS       DASJPC010000204         1664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJPC010000204 DASJPC010000000
VERSION     DASJPC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.213"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4A13_002889"
     CDS             131..505
                     /locus_tag="U4A13_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN1781129.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4A13_002890"
     CDS             complement(619..1248)
                     /locus_tag="U4A13_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN1781130.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U4A13_002891"
     tRNA            complement(1382..1454)
                     /locus_tag="U4A13_002891"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DASJPC010000205         1646 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJPC010000205 DASJPC010000000
VERSION     DASJPC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.214"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            535..>1646
                     /locus_tag="U4A13_002892"
     CDS             535..>1646
                     /locus_tag="U4A13_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781131.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRR"
BASE COUNT          539 a          225 c          226 g          656 t
ORIGIN      
        1 tttgacggct tcggaagata gtttttcaac tctttcaatg tacgaatcat atacctaaaa
       61 taacaatggg actgaagaat caattttgat tcatcagtcc cataaattat agagcctata
      121 ttttgcgaat tgcgctacgg tttcttcttt tcttttgtct gtaatatgaa tatatagatc
      181 cataatgatt tgaatagaag agtggcctaa acgctcttgc acatctttaa tatttgcact
      241 agcttgctaa aagcaagcta gcatgtgtgt gtctaagacc atgaatagta attcgtttaa
      301 gattgttttt cctgatgatt acatctaacc attttcgaga tttagataat tgaaggtgtt
      361 catttctttc tggtaaaaat ttaaatatat aactacattt tctctactaa ctttgaaaca
      421 ctacttttgt aatataaaaa atattttatt aataataaaa ttaatcacat tttatatagt
      481 gagtaatcta gtgttttaaa aaaataaatg atactatatg taatataaaa aaacatgagg
      541 tggtatatga atttgaaaaa aattttttct gtaaaaatat ttatagttta tgcatttgtt
      601 agtttattat atttatttcc agcatttaaa aacatggaaa tatttggctt ttatgatacg
      661 gcatttcaca ttaatcgtgc gttaagttta gaatctattt ttagctcccc tattaatttt
      721 gaaacgttta gatcatatgg catgcaagta aataattttt acccatggtt aactctatat
      781 cctttatttt tattgatcaa gtttactaat ttagcaatag gctataacct ttttctttat
      841 attgttacgc tcattactct atttatatgt cattatgtaa tgtatgaaat taccaaaaaa
      901 catgttacta gctccttttt cgctattata tatacaacat cttcttttag gagtgtagaa
      961 atatttcttc gaggggcaat gggggaatta ctcgcaatgt caatacttcc tcttatttta
     1021 ctcgggttta taaagcttta cgactcaaaa aaagaatctt gggttatgtt ggcgattagc
     1081 atgactttat taatttacac tcatgttctt tctgtagcta tgactatggt aatgattata
     1141 attgctttaa ttattcaatt ttatcggaaa aagaaaatag aaatcttctt aattattaaa
     1201 ttaataaaag ctgctatagt tactttactt ctttcactag cgtttatagg tcctatgatt
     1261 gagcagacat tgtatcaaga tctgaataga ccttatgtgg atatattaca aaaaagagca
     1321 atttatctgg gtaaagagtt tttgattgga agtatagatt cagaattact atcatttggt
     1381 attggcttaa ctttgcttat ttgtttaatt atactaacat ttaactttaa aaaattagga
     1441 atcttatcta aaatcactta tataatgggt ataatatcta ttctactttg tacaaaaata
     1501 tttccatggt ttgccttaca aaatactcct ttaaatatta ttcaagctcc ttggcgtttt
     1561 atgatatttt caacattgtt catatctttt tcagtatctc tatttatttc tccacagttg
     1621 gaaaaatttt cttatacaag gagaag
//
LOCUS       DASJPC010000206         1634 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJPC010000206 DASJPC010000000
VERSION     DASJPC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.215"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            101..868
                     /locus_tag="U4A13_002893"
     CDS             101..868
                     /locus_tag="U4A13_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN1781132.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="U4A13_002894"
     CDS             1060..1458
                     /locus_tag="U4A13_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEN1781133.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DASJPC010000207         1573 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJPC010000207 DASJPC010000000
VERSION     DASJPC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.216"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DASJPC010000208         1554 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJPC010000208 DASJPC010000000
VERSION     DASJPC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.217"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            132..344
                     /locus_tag="U4A13_002895"
     CDS             132..344
                     /locus_tag="U4A13_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781134.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="U4A13_002896"
     CDS             506..1024
                     /locus_tag="U4A13_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEN1781135.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="U4A13_002897"
     CDS             1031..1543
                     /locus_tag="U4A13_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781136.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          611 a          181 c          242 g          520 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agtt
//
LOCUS       DASJPC010000209         1501 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJPC010000209 DASJPC010000000
VERSION     DASJPC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.219"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            139..477
                     /locus_tag="U4A13_002898"
     CDS             139..477
                     /locus_tag="U4A13_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781137.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="U4A13_002899"
     CDS             complement(682..1257)
                     /locus_tag="U4A13_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN1781138.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          465 a          312 c          255 g          469 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgggtaaac
     1501 g
//
LOCUS       DASJPC010000210         1474 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJPC010000210 DASJPC010000000
VERSION     DASJPC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.220"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            286..693
                     /locus_tag="U4A13_002900"
     CDS             286..693
                     /locus_tag="U4A13_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781139.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            707..1108
                     /locus_tag="U4A13_002901"
     CDS             707..1108
                     /locus_tag="U4A13_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781140.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1110..>1474
                     /locus_tag="U4A13_002902"
     CDS             1110..>1474
                     /locus_tag="U4A13_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781141.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          566 a          180 c          258 g          470 t
ORIGIN      
        1 gaggggaagt acatatagtt gttgatatag ttgctaatat tgttagctaa tttgttagta
       61 aaactgtata cgaatttaga tagtaaaaaa acagtttgtt ccttatatag aggcactttt
      121 actactgatt aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta
      181 gctaatattt ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag
      241 tgaaacataa atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta
      301 caattaataa tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa
      361 gtaattaatc cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct
      421 gtaacagctg tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa
      481 gttttaaaca agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct
      541 cctcaagtta acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca
      601 ggagagcatt atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat
      661 atcaaggtca acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt
      721 accatcaaat acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc
      781 atcggtaatt ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa
      841 tgataatcaa cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg
      901 caaaattttt gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata
      961 tgatgaagat ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc
     1021 agattacgat gttttggata ctctcgataa tgtattatct gactataaat atatggggaa
     1081 atctgtagta gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt
     1141 ccaaacaaat tgaatcagat gataaatata attctaaaga atcttataga agaactgctg
     1201 tttcaagagc atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg
     1261 atattattta taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt
     1321 caacagttaa ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga
     1381 aaagaaaatg tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga
     1441 tgaatcgaga agcagaaaaa ataataaaca aact
//
LOCUS       DASJPC010000211         1451 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJPC010000211 DASJPC010000000
VERSION     DASJPC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.221"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..625)
                     /locus_tag="U4A13_002903"
     CDS             complement(<1..625)
                     /locus_tag="U4A13_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN1781142.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="U4A13_002904"
     CDS             complement(784..1050)
                     /locus_tag="U4A13_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN1781143.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="U4A13_002905"
     CDS             complement(1040..1396)
                     /locus_tag="U4A13_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1781144.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DASJPC010000212         1424 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJPC010000212 DASJPC010000000
VERSION     DASJPC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.222"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..276)
                     /locus_tag="U4A13_002906"
     CDS             complement(<1..276)
                     /locus_tag="U4A13_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEN1781145.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAI"
     gene            401..1186
                     /locus_tag="U4A13_002907"
     CDS             401..1186
                     /locus_tag="U4A13_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEN1781146.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            1179..1421
                     /locus_tag="U4A13_002908"
     CDS             1179..1421
                     /locus_tag="U4A13_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781147.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          534 a          231 c          215 g          444 t
ORIGIN      
        1 aattgcatat cccaaaatat caacgattgt ggcaatcaga tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caatcgatcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
      361 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
      421 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
      481 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
      541 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
      601 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
      661 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
      721 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
      781 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
      841 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
      901 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
      961 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     1021 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     1081 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     1141 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     1201 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     1261 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     1321 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     1381 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DASJPC010000213         1419 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJPC010000213 DASJPC010000000
VERSION     DASJPC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.223"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..265)
                     /locus_tag="U4A13_002909"
     CDS             complement(<1..265)
                     /locus_tag="U4A13_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781148.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSV"
     gene            complement(267..1325)
                     /locus_tag="U4A13_002910"
     CDS             complement(267..1325)
                     /locus_tag="U4A13_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN1781149.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          556 a          233 c          173 g          457 t
ORIGIN      
        1 aaacggataa atcatccgag aaaaattgat tatggttagt taacaagtta attttacgtt
       61 taaaggaaga accgattttc cggtgattca caaaaacaac atcattaaat agccctaatt
      121 cttttatacg ctcactatat cttctagcgt tttcaaattc agaataaata aataaatctc
      181 cttttgattc tcggtaaaaa ttttgataaa catttaatat attaatcaaa tgtaaaggag
      241 tccaagcaat gaaagctgtc ttcatattaa ttctcctttt ttaaatacaa gtaagaaatg
      301 cttcccaaat aataccagaa aacaattgaa atgacatcgc gcttattgaa aagtacatga
      361 gcctccacca tttcatctat aaaaatagat agtataaaaa ttaaaacaat accaagaaac
      421 gcataattca ctttacttct aaaaaatgtt ttgagtaaac taaagatatt taacacatag
      481 aaagcataga tacatataaa agcaattata ccacttgata caagtacttg aagaaatcca
      541 ttatgcatgt ttccatgtgc tctttttagt tcattcacat ctgtagtatc aagatctact
      601 tttttcaaaa attttgcatc gctattccca tagaaatcgg tctctccata accaaataac
      661 aaagttggtc ttataacttt aagcgcagct ttccaaatcg taaaacgacc agctgaaatt
      721 tcggagttat cactagaatg ctgtaatatt acttcttttt ctggaagtgg agttttatct
      781 gattcatttg actctataac ttctactttt ctatcaatcg atacaggaat tgttgctaaa
      841 aaatccttag cataattagt accaaaagaa aaatatacca atagaactaa taatatgctt
      901 accatccatt tgatttttac aggtagtact tctctaatgc tctttctaaa ttttgaattg
      961 gcaaacaaca aaagaaataa tactaaaaaa gaacaaatta ctacctgggc acctctagac
     1021 gaagaaagtg taatataaaa tatttgtacg ataaaattca tcatataaaa aacacgaatc
     1081 gtttttgtta tttttgaaat taatacacta attaaactta aaataataga agtatatcca
     1141 aacatagctc caacatttgg acttgtatag acaccaaata accgatgttc aataaaacct
     1201 tgtcttgcaa gtaatcctgg cgttcctgtt gggacatgat aagaaatcaa aaaaataaat
     1261 tgatagatag atattagcgc agctaaaaaa attatgataa tgaaaacatt attaaaatac
     1321 accatttttt tctttttttc ctcttcactt tgtgaaaagt ctgcaggaaa aattactatc
     1381 aaagtaatca gcaaatatcc aaaattataa atagatgga
//
LOCUS       DASJPC010000214         1418 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJPC010000214 DASJPC010000000
VERSION     DASJPC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.224"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(150..1337)
                     /locus_tag="U4A13_002911"
     CDS             complement(150..1337)
                     /locus_tag="U4A13_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN1781150.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          413 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttca
//
LOCUS       DASJPC010000215         1399 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJPC010000215 DASJPC010000000
VERSION     DASJPC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.225"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1037
                     /gene="thiI"
                     /locus_tag="U4A13_002912"
     CDS             <1..1037
                     /gene="thiI"
                     /locus_tag="U4A13_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN1781151.1"
                     /translation="QLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTGK
                     ETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAYL
                     SYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQA
                     LQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIRR
                     IRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTFN
                     LSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESSN
                     EKTEEFSELL"
BASE COUNT          483 a          228 c          295 g          393 t
ORIGIN      
        1 ggcagctagt tattccaaaa ctagcaaaaa tatttgggat ccaaaatttc tctccaagta
       61 ttcgtgtaga aaaagatgtc gaagttttga aacaagctgt tcaagacatc atgaaagaaa
      121 tttatactgg gaaagaaaca tttaaaatta tcgctaaaag aagcgatcac caatttgaat
      181 tgacaagtaa tgaattgaat caaacattag gtaatgctgt ttttgatatt ttcccgcata
      241 tccaagttca aatgcgtcaa ccagatattc cattacgtgt agagattcgg cgtgatggcg
      301 cttatctatc gtatgaaacg atcaagggag ctggaggatt gcccgtagga acaagcggaa
      361 aaggaatgct tatgctttct ggggggattg attctcctgt agcagggtac tttgccatga
      421 agcggggagt ggaaattgaa gctgttcatt ttgctagtcc gccgtataca agtgaacaag
      481 cactgcaaaa agcaaaagat ctaacagcca aactagcacc ttatgtggga acgatccatt
      541 ttatcgaagt gccatttacc gagattcaag aagaaatcaa aaagtcagtg ccacaaggat
      601 attggatgac aattactcgt cgaatgatgt tgcgtttgac cgatgaaatt cgccgtattc
      661 gtcatggttt agtcattttg aatggtgaat cattaggtca agtggcttca caaactttgc
      721 aaagtatggt ggctatcaat gaagtaacga atacgccaat cattcggcct gtagcgacaa
      781 tggataaact tgaaatcatt gaagtagcag aacagatcga tacatttaat ctttctatcc
      841 agccttttga agattgctgt acgatttttg ctcctccaca gccaaaaaca cgccctcaac
      901 tggaaaaggt ccatcaatat gaagagcgtt tagcaataga tgaaatgatc caacgagcac
      961 tagctggctt gaagatggaa gagatacaag cagaatcgtc taatgaaaaa actgaagaat
     1021 tttcagaatt gctataagag gttatgaaaa tagtgttttg accagagtat tagagaaaca
     1081 aaacgaaaaa atagctccat gtattttacc gtttttgaac taatgtcgaa gattagctga
     1141 ttgaatacca tttatcaaga agttatggaa aagccggttc ttttttacta actgaaaaat
     1201 aaaattttat ttagagatca agctacagtt gataaatgat ataaaaaaga gaatttccca
     1261 tgaaatagcg aggttgtgac agaagtgggc agcttcaaga aataagaagg aatttacgaa
     1321 aattgctttt caaatttttg tgaaatttcg gcttattttt cgaagaagtt acttctggaa
     1381 caccgtgtat cagaggttg
//
LOCUS       DASJPC010000216         1373 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJPC010000216 DASJPC010000000
VERSION     DASJPC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.227"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            191..445
                     /locus_tag="U4A13_002913"
     CDS             191..445
                     /locus_tag="U4A13_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN1781152.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="U4A13_002914"
     CDS             446..763
                     /locus_tag="U4A13_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN1781153.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DASJPC010000217         1355 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJPC010000217 DASJPC010000000
VERSION     DASJPC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.228"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1355
                     /locus_tag="U4A13_002915"
     rRNA            <1..1355
                     /locus_tag="U4A13_002915"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DASJPC010000218         1320 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJPC010000218 DASJPC010000000
VERSION     DASJPC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.230"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(31..>1320)
                     /locus_tag="U4A13_002916"
                     /note="aph(2'')-Ia"
     CDS             complement(31..>1320)
                     /locus_tag="U4A13_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEN1781154.1"
                     /translation="TEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEI
                     VYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSG
                     FRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGS
                     GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE
                     LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ
                     NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDG
                     NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQD
                     IVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          436 a          226 c          100 g          558 t
ORIGIN      
        1 ttttataata gcttttcata tattatataa tcaatcttta taagtccttt tataaatttc
       61 ttttctacca ttttcgataa attcctgttt aatattttta attccataaa caatagtttc
      121 aataggataa tattcttcaa ctatatcttg atattctttt gctttctcaa tatctatatt
      181 tccatacatt cttaatatat cttctccaaa atttgttcct atttcttctt cactatcttc
      241 aagtaagtat ataaaatcac aatattcatc tataattcca gaatctccaa aatcaattat
      301 tccagttaat ctattattgc catctaacaa tagatgatta caactaaaat cattatggca
      361 taaacacttt ttaccctcaa aaactgttgt tgcatttagt ctttccataa aactttctat
      421 ataatctttt tctatatcag ttaaatcatt ataaatagtt tcacgcaaca atatatactc
      481 ttctaataca ttttgtttat tatcaatagt acattcacta atatctgtat aatctaaacc
      541 gtgcatttgt cttaaaaaac tggcaatatc tcgttttaac aaattttgtt cttcttctga
      601 catagtagaa taaatttctg gtgttaaaaa agttccttta atttctttat aacctagtat
      661 agataattca tcactaatat acgaatattc aatattagga atttttacat tagtttctaa
      721 atttgtattt aaaaaattat atattgcttt ttcttttgca taaccttttt tcttattagt
      781 actaaatttt gttttaaaaa tgtattcatt attaactaaa tatgccacac tatcataacc
      841 actaccgatt atttcaatac tatctacttt gaaattatca aagtaatgct caattaaata
      901 tttcattgcc ttaacatttg tggcattatc atcatatcta tattccatta aataacaatc
      961 ttcttttttg ccctcgtgta attcatgttc tggcaaatct tcaataattc taaaaccaga
     1021 tttttggtat gcccttattg ctcttggatt atttttatga gggtctaaaa taactgcatt
     1081 agcatttctt tcttttttca aaaattcaaa aatcaattta atatatcttg taccaattcc
     1141 tttactccaa taatttggct ctcctataaa ttgatccata ccatagacta tctcatcagt
     1201 ttttggataa tgataatcag tatataactc atcatacatt ttatatattt gtccatatcc
     1261 aataggaaca ttgttatatt caataattac tctaaaaact tcatcttccc aaggctctgt
//
LOCUS       DASJPC010000219         1286 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJPC010000219 DASJPC010000000
VERSION     DASJPC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.231"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>1286
                     /locus_tag="U4A13_002917"
     CDS             <1..>1286
                     /locus_tag="U4A13_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN1781155.1"
                     /translation="EFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTYQPEE
                     CFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAEDSVSD
                     RRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQPLPEV
                     VCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQVRLV
                     VSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRSKHLK
                     KYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVFLSAI
                     RRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLNNHIK
                     VLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQL"
BASE COUNT          402 a          284 c          256 g          344 t
ORIGIN      
        1 agagtttacc aaagagttac tagatattct agacctaaat cttacctttc atgaagatgc
       61 ctttcgcaaa gagcgcatta atgacgaaac atgttttgtt tttgatggca cattgaccta
      121 tcaaccagag gaatgcttcc attgccacta tcaaaataag caaacgatca ttaaatgggg
      181 ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca ttgtgcggat
      241 taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt
      301 tagcgatcgt cgctgctcca ttgcccgcag agtcaaacaa gccattcttg agctattaag
      361 tgagcctatt tctatgtctt tgattgcacg tatgaagcac atttctccga ccactgtcat
      421 tcgaatactt cgcagccttc ggcctaaaac cgtttctttg aaccagccgt taccagaagt
      481 ggtgtgtttc gatgaattca aatcggtgaa aaacgtctcg ggagccatga gctttgtcat
      541 gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac tcaatccttt
      601 acgcgactat ttcttgcgct accctagaaa agtccgtgaa caagtacgtc tagtggtttc
      661 agatttctat acaccctatc gcacattggt gaaagagtgt tttccgaatg cccgaatcgt
      721 cgccgatcgc tttcatatta gccaacacat cggtcgggcc tttaccaacc accgaattca
      781 agtaatgaag acctttaaaa aaggggatcg tcggtcgaag cacttaaaga aatactggag
      841 actacttcaa aagaatgcgt gggaattgaa tggtcagcat cgctactggc gtccgtcatt
      901 tcgagatcat ctgacagaag cggagatagt cgatcggcta ttatattatg atgactcctt
      961 aaagcgaggc tacgaagtct accaggtttt tctttccgct atcagaagac aagatgttcc
     1021 tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact atcaaccagt
     1081 gttcacgacc ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag ttccctattc
     1141 caacggccct atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa ttgcctatgg
     1201 ctttagaaac tttcaaaact accgagaacg aatcttttta tatcgaggaa aatatttcaa
     1261 gaaaactaag aacactaccc aactga
//
LOCUS       DASJPC010000220         1270 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJPC010000220 DASJPC010000000
VERSION     DASJPC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.232"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            265..957
                     /locus_tag="U4A13_002918"
     CDS             265..957
                     /locus_tag="U4A13_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN1781156.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          389 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc
//
LOCUS       DASJPC010000221         1267 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJPC010000221 DASJPC010000000
VERSION     DASJPC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.233"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1101
                     /locus_tag="U4A13_002919"
     CDS             22..1101
                     /locus_tag="U4A13_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1781157.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          301 c          310 g          278 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga
       61 aaacatcttt cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc
      121 aaccgtcagg ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat
      181 cgcggaacca tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac
      241 tattctgttt atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt
      301 ggcagacgcc ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg
      361 ttaaatgaaa aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc
      421 gattgtgcca tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg
      481 cgaacaaaga acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca
      541 ggacaccgtc ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt
      601 gaaacacggg aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag
      661 tcggatgccg tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag
      721 ttgaacggga aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct
      781 ggcgctaact tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct
      841 gggctacacg atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct
      901 tgggagcgtg ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga
      961 agcccgatta gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat
     1021 tacccgcgga aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag
     1081 caagaacggt tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg
     1141 cttcatgtct ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt
     1201 cattatcaag gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt
     1261 tacctgt
//
LOCUS       DASJPC010000222         1263 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJPC010000222 DASJPC010000000
VERSION     DASJPC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.234"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..1101
                     /locus_tag="U4A13_002920"
     CDS             22..1101
                     /locus_tag="U4A13_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1781158.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          335 a          342 c          356 g          230 t
ORIGIN      
        1 aatagacaag gatgaattca catgacgcaa aaccatgata acacagtttc acgcaaggga
       61 aaacacctaa cctactcaga acgttgccaa atcgccatcc tgaaaaagga aaactattcc
      121 aaccgtcagg ttgcggatgt cctgggctgc gtgccgcaga ccataaacaa cgaaatcaaa
      181 cgcgggaccg ttactcagct taaccgccag aatcagaacg ggaaggtcta cgactattat
      241 catgacgtct acgacgccga tgccgggcag gcggcctacg agaagaaccg cttgaactgc
      301 ggcagacggc ccttatgggc cgacacggac gccttcattg aatgggcgga cgacatgatg
      361 ctgttggaaa aatggtcccc ggacgtggtg gtgggctttg ccagaaaaca cgaattgttc
      421 gacccggcca ttatccctgg gaccaccacc ctctatcagt ggatcgaccg tggcatcatg
      481 aaaacgaaga atatggacct gctagaaaaa ctgtcccgca aaccgaaatc gtcccgtcat
      541 ggcaaccgcc cgaataaacg ggtgctaggg aaatccattg aagagcgccc ggaggcggtt
      601 ggtacccgga aaaccttcgg ccattgggaa gtcgacacgg tcgtaggaaa caagaccaag
      661 tcggatgccg tgctgctgac gttggtggaa cggcagacac gcttcgaggt cattctgaag
      721 ctggccggga aggatgccga atcggtcaac caagcggtcc gacagctgag agaacgggct
      781 ggtgcgtact tcggccagct gttcaagacg gttacctcgg acaacggctc ggagttcgcg
      841 ggactgcacg atgccttgca ggatgtggca aacatctact tcagccaccc ctatgcttcc
      901 tgggagcgcg ggacgagcga aaaccaacac aagctgattc gccgtttcat ccccaagggc
      961 agcccaatcg gcaacgtgag cgaaaaccaa atcttgcgga ttcagcgttg gatgaacgat
     1021 tacccgcgaa agattttggg ctatgcaacg ccgcatgaca cgttcgtcca ggcatttagg
     1081 caggaacggt tgacggcttg acacgtgggc ccttgacaac gagcctccct cggcatggct
     1141 tgtgtctgga aagctggccg gggccagcca gtcgggagac taccatgcct cagctcattg
     1201 tcaagggccc actcgattag aaaaattaga cggttggtgg taacgagcaa ccgggtcacc
     1261 atg
//
LOCUS       DASJPC010000223         1246 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJPC010000223 DASJPC010000000
VERSION     DASJPC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.235"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U4A13_002921"
     CDS             200..742
                     /locus_tag="U4A13_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781159.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="U4A13_002922"
     CDS             898..>1246
                     /locus_tag="U4A13_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN1781160.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DASJPC010000224         1226 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJPC010000224 DASJPC010000000
VERSION     DASJPC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.236"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            2..>1226
                     /locus_tag="U4A13_002923"
     CDS             2..>1226
                     /locus_tag="U4A13_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN1781161.1"
                     /translation="MITEVSYETLQKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAI
                     IVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRY
                     KIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHA
                     SIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLT
                     KRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNR
                     ANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPD
                     LFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVS
                     YGFKSFENMRIRIFLI"
BASE COUNT          403 a          244 c          238 g          341 t
ORIGIN      
        1 gatgattaca gaggtctctt acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc
       61 tgttctctcg cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa
      121 cgggaaagca attatagtga aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata
      181 caatcatatg cctttggtta tgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg
      241 cacccattgg acggctcaaa gttattttgt ccaacccaga cattcaatcg caaatcatgt
      301 tagatataaa attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaag
      361 ctgtcaggta tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc
      421 aaagcaatct aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc
      481 ttccatagaa gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga
      541 tgttttgcct acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc
      601 agaagaagta gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg
      661 tgttctgcca aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca
      721 agcgttcaat gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag
      781 ccaggcagaa aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa
      841 ccattatgaa tacaaaacat ggaaaagttt ccgagcacca aaatacccat ttcttactga
      901 agcaatgatg attgatcgat tgcttgagtt ttctccgccc ctaaaggagg cgtatccctt
      961 ttttcatgaa ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt
     1021 ggcagaactt cccgaaacgt tagatgacag ctttcgggaa aagcttcaaa accttctgac
     1081 ctatgaagaa ggcatcacca acgcaatgat ctatccttat tccaatggaa aaatagaagc
     1141 gaagaatacc cacataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa
     1201 catgagaatt agaatctttt tgatca
//
LOCUS       DASJPC010000225         1215 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJPC010000225 DASJPC010000000
VERSION     DASJPC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.237"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            414..947
                     /locus_tag="U4A13_002924"
     CDS             414..947
                     /locus_tag="U4A13_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN1781162.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DASJPC010000226         1212 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJPC010000226 DASJPC010000000
VERSION     DASJPC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.238"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..607)
                     /locus_tag="U4A13_002925"
     CDS             complement(<1..607)
                     /locus_tag="U4A13_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1781163.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINS"
     gene            complement(607..>1212)
                     /locus_tag="U4A13_002926"
     CDS             complement(607..>1212)
                     /locus_tag="U4A13_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN1781164.1"
                     /translation="IQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNFFVSNKKTN
                     GCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVD
                     VVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTII
                     EDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          414 a          208 c          136 g          454 t
ORIGIN      
        1 tactgttaat ttcaagattc gaatatttac caaactgttt ttttactgat ttagggaaac
       61 caaataggtt tataatataa tccaattcat gtatacagtc taattctact cctcccccca
      121 tagatttaaa ggcagaataa ttatttctat aatcagtacc ttttctccaa tcaggaagat
      181 aagtagaaca aatagctctt acagaatata cttttttact ttgaatacta ttacgaacaa
      241 cttccatgat atttttatac ctcagtggag cagcaacata aatatttttg taatcataat
      301 tatattgagt aagatcaata ctctcagaaa aagcaggttt ttctaaaaat atgtaatcaa
      361 ctttttcaac aattacattt agtgtttcca gatgcttaaa agtcggattt gtaataaaag
      421 ctatatcata atgtgtatcc aaatcgtctc tattttgaat atacgttatt tcttcaaact
      481 ctttttttcc actgctacta taagctgtta tatcaaaatt aatagaattc ttttttccat
      541 agcttattag attttttaga tgtctctttc caattgaccc taacccaaaa aaaattactt
      601 tcatcattaa tttacctcaa tgtcgttaat taagttaata atatacttat ctttttcaaa
      661 agtatattgg ggttccattt tcttttccaa aagcgtgaaa aattctttaa tctcctctaa
      721 atatgcatct tcaataatag tatgtgaata attattatca tgcataacct catcgtacaa
      781 ttgaatatct gtcatactag aagtttcttc attccatgaa aatagagaat ccgggttacc
      841 ttcccattct attaaaatct tttcaccaat tatagttaaa tttcttttag ctactcttga
      901 aacaacatct acacttaaag ttccaactat accagtttca tgctcaataa taagagtgaa
      961 actgtccgaa taatcaatat caagattgga taatttctgg gtcattacag taaacgattt
     1021 aactttccca aatgtatcta taatccaagg taattcaata gcgaatattt ctctacaccc
     1081 attagttttt ttgtttgaaa caaaaaaatt gcgataatct tcccaaggat gccagtcggg
     1141 taaatactgt cctacatggt attgataaga aattttttta ttatgcgtca ctttagactt
     1201 aatatattga at
//
LOCUS       DASJPC010000227         1193 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJPC010000227 DASJPC010000000
VERSION     DASJPC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.240"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U4A13_002927"
     CDS             <1..1076
                     /locus_tag="U4A13_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1781165.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DASJPC010000228         1143 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJPC010000228 DASJPC010000000
VERSION     DASJPC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.241"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="U4A13_002928"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="U4A13_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN1781166.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DASJPC010000229         1126 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJPC010000229 DASJPC010000000
VERSION     DASJPC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.242"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            36..995
                     /locus_tag="U4A13_002929"
     CDS             36..995
                     /locus_tag="U4A13_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1781167.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          400 a          179 c          206 g          341 t
ORIGIN      
        1 agttccgacc aaagaattat aaggagtgag tacaaatgac ctatacccat cttacaacgg
       61 atgaacttgt aataatagag tcttatttca aaatgaatca atctgttgct aaaactgccc
      121 attgcttgaa tcgttcaaga caaacgatcc ataaagtata tctgttcttc aagcaaggaa
      181 aatcagcctt agaatattat caacagtata agaaaaacaa atcaaactgt ggtagacgtc
      241 cgcttgtttt acccgaggaa caatcagaat atattcaaag aaaggttgtt caaggatgga
      301 cacccgatgt gattgttggt cgtgcagcgt ttcctattag ttgttctgct cgtaccattt
      361 atcgtatgtt caaaaagggg ctatttgatt cttctgactt accgatgaaa ggcaagcgta
      421 aaccgaatgg acatcaagaa agacgtggaa aacaaacttt ccgccgctct attcatgaac
      481 gtgaaaagga ttatagccaa ttctcaaatg agtttggtca ccttgaaggt gacactatcg
      541 taggtctgaa acataaaagt gctgtaatta ccttagttga acgattatca aaagttatca
      601 tcacattgaa accgtgtggt agacaagcga ttgatattga aaaaaaatta aatcaatggt
      661 ttgaatctgt accgaaaaac ctattcaaat ccatcacttt tgattgtgga aaggaatttt
      721 caaattggaa acagatcagt aatgtcaatg atattgccat ttatttcgct gatccaggaa
      781 cgccgtctca aagaggccta aacgagaatt ctaacggatt gttacgtaga gatggtttat
      841 tgaaatctat ggatttcaat tcagtagatg aattttttat tcaatctgtc gcatctaaac
      901 gaaataatat tcctagaaaa tcactggatt atcgaacacc tttggaagta tttttgagtt
      961 acgtaagtat tgatgatctg tctaacttaa tttgacaatt aagataaagt aaaaatgcga
     1021 aattaagtag atgagagtga tgatgttcga aatatctatt atttttaata agaatacata
     1081 agctatctag tttgtaaaca atgagagaaa aaacatcaat attttt
//
LOCUS       DASJPC010000230         1116 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.243, whole genome shotgun sequence.
ACCESSION   DASJPC010000230 DASJPC010000000
VERSION     DASJPC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.243"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="U4A13_002930"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="U4A13_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEN1781168.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DASJPC010000231         1105 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJPC010000231 DASJPC010000000
VERSION     DASJPC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.244"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="U4A13_002931"
     CDS             <1..1087
                     /locus_tag="U4A13_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1781169.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DASJPC010000232         1097 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJPC010000232 DASJPC010000000
VERSION     DASJPC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.245"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..153
                     /locus_tag="U4A13_002932"
     CDS             <1..153
                     /locus_tag="U4A13_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1781170.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="U4A13_002933"
     CDS             159..575
                     /locus_tag="U4A13_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781171.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="U4A13_002934"
     CDS             681..944
                     /locus_tag="U4A13_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN1781172.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1097
                     /locus_tag="U4A13_002935"
     CDS             944..>1097
                     /locus_tag="U4A13_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN1781173.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLGSV
                     AKFENQT"
BASE COUNT          416 a          139 c          223 g          319 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcggtt ctgttgcgaa
     1081 gtttgaaaat caaacgc
//
LOCUS       DASJPC010000233         1087 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJPC010000233 DASJPC010000000
VERSION     DASJPC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.246"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1032)
                     /locus_tag="U4A13_002936"
     CDS             complement(<1..1032)
                     /locus_tag="U4A13_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEN1781174.1"
                     /translation="MYLLNITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVK
                     AMGIAGIISTIVTIIANIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDF
                     EAKDPQLKREMLNYSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQII
                     NTLQGIFIQAWQISAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILY
                     AKDFYEAWKYVPFLLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVL
                     VYKIGVQGATIATVICSYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFK
                     NYVIEIIIMI"
BASE COUNT          406 a          203 c          126 g          352 t
ORIGIN      
        1 aatcataatt ataatttcta ttacataatt tttaaaatat atttctatga ttgcctgaac
       61 aaacaataat ccccaagatt ccacaacaat atgataatgc tttataaaaa tatcttttcc
      121 cacagctcgt ttacgaacaa catatattac atatgaacat ataacagtgg ctattgtggc
      181 accttgaacc cctatcttat aaacaaggac tacactcata atcacattaa ctaatgcccc
      241 tatgattgca gaccacatca tggatttaga atcttttttt gctgataaga taggtcctaa
      301 cagacctgat gcacaattta aaacattagc tattaataaa aatggaacat atttccacgc
      361 ttcataaaag tctttcgcat aaagaatatg tgaaaacggt ctagttaata tgataagact
      421 tgaacaggtt atgcacatta acaaattaat cataacaaat gtatttccat aaaattttgc
      481 agtctcatta tctccatatt ctttaattgc agaaatttgc caggcctgta taaatattcc
      541 ctgcaaagta ttaattattt gaggtatttt atatgccaca gacaataagc cattcgccga
      601 gacacctact ataaaggaaa ccacatacct atcagctgta ctattaatcc accatccaac
      661 aacagttgca attaatggaa tcgagtaatt tagcatttct ctctttagct gaggatcttt
      721 agcctcaaaa tcgatatatt cccatattct cactaatact gacagataag tagctgaaat
      781 aatctgtgat aaaatatttg caatgaaaaa accaattaat ccccatttca gaataagcaa
      841 gaagaaaata ttggcaatta ttgttacaat tgtgctgata atacctgcta tgcccatagc
      901 tttaactttt tctaaccctt tagcaaattg tattagaaat tgattccata cataggatat
      961 ataatacaaa aaaatatatt tttctaatcc ataaacatct tgccataatt ttgtaatatt
     1021 taacaaatac attacggatc caaagactat cacgcttaaa aatataactt taatcgctat
     1081 agatgct
//
LOCUS       DASJPC010000234         1069 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJPC010000234 DASJPC010000000
VERSION     DASJPC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.247"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..969
                     /locus_tag="U4A13_002937"
     CDS             <1..969
                     /locus_tag="U4A13_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN1781175.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DASJPC010000235         1062 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJPC010000235 DASJPC010000000
VERSION     DASJPC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.248"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..963
                     /locus_tag="U4A13_002938"
     CDS             4..963
                     /locus_tag="U4A13_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN1781176.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          369 a          200 c          210 g          283 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc ca
//
LOCUS       DASJPC010000236         1061 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJPC010000236 DASJPC010000000
VERSION     DASJPC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.249"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..995)
                     /locus_tag="U4A13_002939"
     CDS             complement(39..995)
                     /locus_tag="U4A13_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781177.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg t
//
LOCUS       DASJPC010000237         1052 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJPC010000237 DASJPC010000000
VERSION     DASJPC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.250"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="U4A13_002940"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="U4A13_002940"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN1781178.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          361 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt tt
//
LOCUS       DASJPC010000238         1030 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJPC010000238 DASJPC010000000
VERSION     DASJPC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.251"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..943
                     /locus_tag="U4A13_002941"
     CDS             <1..943
                     /locus_tag="U4A13_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN1781179.1"
                     /translation="CYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMTKN
                     ITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMILE
                     LIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYCHL
                     YDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSRDF
                     VYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEERS
                     GDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          329 a          162 c          221 g          318 t
ORIGIN      
        1 ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca atcatttagg
       61 agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag aaaatcttga
      121 tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct tgatggaaga
      181 gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg ctagtgtggc
      241 agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg tattaatgat
      301 tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta gttcttcagc
      361 ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga ttcgcccatt
      421 gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt attgtcattt
      481 atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa atcaaaatcc
      541 gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga aacaaatcgt
      601 aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag actttgttta
      661 tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg cactggggaa
      721 acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc atgcaatcaa
      781 tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg gtgatatcca
      841 tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga aatatggtat
      901 tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac taagataaga
      961 ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta aaatcaaatc
     1021 tgggatagaa
//
LOCUS       DASJPC010000239          997 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJPC010000239 DASJPC010000000
VERSION     DASJPC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.252"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..913)
                     /locus_tag="U4A13_002942"
     CDS             complement(26..913)
                     /locus_tag="U4A13_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN1781180.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DASJPC010000240          994 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJPC010000240 DASJPC010000000
VERSION     DASJPC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.253"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>994)
                     /locus_tag="U4A13_002943"
     rRNA            complement(<1..>994)
                     /locus_tag="U4A13_002943"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DASJPC010000241          994 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.254, whole genome shotgun sequence.
ACCESSION   DASJPC010000241 DASJPC010000000
VERSION     DASJPC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.254"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..555
                     /locus_tag="U4A13_002944"
     CDS             <1..555
                     /locus_tag="U4A13_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781181.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRN
                     ISHKVRVNNRKEVCDDFKMVYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEGELEQMLSQPMEN"
BASE COUNT          324 a          176 c          188 g          306 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttttcgta atatcagtca caaagtgcgt gtcaataatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tagaagcacg tggtgctttt
      241 gcgaaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataagca aatcaagaaa tacagccacc gcaaggaaca gttccaaaac
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctgagtcaa
      541 ccgatggaga attagaaaca atctacagta gggaagaacc atttacacaa aattattgac
      601 gctcccaagc taaaactcct attaatcgat ctactaaaaa taaataaaaa aattcttttc
      661 aatttttatt tatggtatga cataaataaa gtaaagttat tcttctaggt taatcttatt
      721 aatactcttt ctcactagtg ttgcatgcaa cactagtgag aaagagtatt aaatatactg
      781 caaggcaaga catttggtaa ttgaagaata acaagcaaaa aaatagcagt caggtctctt
      841 gacaaagtat agttgctatt ctttttttag gccctacttt aatgaaattt ttgtttggag
      901 ggaacgactt cattcctttg attatttcag ttgaatttaa tagagaagcc tggcgtctat
      961 taagaaatac ttttcttgat gagcgtcaat tttt
//
LOCUS       DASJPC010000242          986 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJPC010000242 DASJPC010000000
VERSION     DASJPC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.255"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..704
                     /locus_tag="U4A13_002945"
     CDS             <1..704
                     /locus_tag="U4A13_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN1781182.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DASJPC010000243          965 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJPC010000243 DASJPC010000000
VERSION     DASJPC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.256"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..690
                     /locus_tag="U4A13_002946"
     CDS             19..690
                     /locus_tag="U4A13_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN1781183.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            696..>965
                     /locus_tag="U4A13_002947"
     CDS             696..>965
                     /locus_tag="U4A13_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781184.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
BASE COUNT          344 a          153 c          204 g          264 t
ORIGIN      
        1 aaatggaggc agaatttgat gagtgaaatc aaagggacaa cgaactttga aaaacttttt
       61 agtcgtaagt taaataaaat tctcaagaaa aaaggaaatt ttgattattt atcttgggct
      121 cacgcgtggg agattatgaa aaagaatgat ccacaggcaa cggtaactat taatgagtat
      181 aaacactaca gggttgtttc tggaactcat caagactttc ttgttgagga atataaacct
      241 tttcttatgg atgaaacagg gacttatgta tctgtctcag taacggttaa aggacatacg
      301 gaaacagagt tatttcctgt tttagattat cgaaaccaac cagttgttaa accaaatgca
      361 atgcaaatca ataactcatt gaagcgatgc tttgtgaaag cattggctct acacggactg
      421 ggattatatg tatttcaagg ggaagatatt ccaacaccac ctagaatcga tacaaagaaa
      481 ttaaacatgc tagagacgat tctagaagct ttcaatgagc agatgggtaa agatatgacc
      541 aaaaccttaa tcgaatatgt taatgagcag acagataaat tagggctctt agctgataac
      601 gttgaaacta ttgaacagtt aagctatgag caatgtgcct tgatggagcg agcaatagca
      661 gctaagagaa aagaattaga taagaagtga tatgagtgtt taaaccatta atcgattcat
      721 attcagcggt tctgaaaaag ttcaaaggaa aagacataag cgcaaccatc aatgaggaag
      781 tgaacattga tcgactaaag acgatgtatg acggctacga tggtgatcga gtcattgaaa
      841 ttcgttttat tgatcctaga cgtttcaccg tacagcaacg aaacttcatc tatgcgctga
      901 taggcgatat ttttatcgat acaggcatgc caacggactt ctggaaggaa ttcttctact
      961 tccgt
//
LOCUS       DASJPC010000244          890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJPC010000244 DASJPC010000000
VERSION     DASJPC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.257"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..888
                     /locus_tag="U4A13_002948"
     CDS             <1..888
                     /locus_tag="U4A13_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN1781185.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DASJPC010000245          833 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.258, whole genome shotgun sequence.
ACCESSION   DASJPC010000245 DASJPC010000000
VERSION     DASJPC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.258"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..789
                     /locus_tag="U4A13_002949"
     CDS             <1..789
                     /locus_tag="U4A13_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN1781186.1"
                     /translation="DKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDLEFK
                     NKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIEIVD
                     SLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILDENV
                     YIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIFDVK
                     FDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
BASE COUNT          372 a           56 c          119 g          286 t
ORIGIN      
        1 gataaaatga tgaaacttga taaaaaaatg agtttaaaag ttagctctaa tgatgaagtt
       61 ggagaattaa aacaacaaat taatgatttg tattctactt tattaagaac aattgacgat
      121 ttggaattta aaaataaaga aattttaaaa ttagaaaagt taaaatatga cttttttaaa
      181 ggtgcatccc atgaacttaa aacccctctt gctagtctta aaataatact ggaaaacatg
      241 aaatacaata ttggaaaata taaagaaaga gatatttata ttaatgaatg tattgaaatc
      301 gttgatagtt taacaaaaaa tatttctcaa atattatcag ttcattctat agaaaatttg
      361 aataatgatg aagaatattt gaaaataaat gatacattag aagacatatt aaaaaagtat
      421 gaaatattag caaatcaaaa gaaaataact gtcaataatt atatattaga cgagaatgtt
      481 tatataggaa aaacagcttt aaagattatt ctatctaatt tgattagtaa tgcggtaaaa
      541 tatactgatg taaatggggt aattaatatt gggatagtta atgattggtt atatatagag
      601 aattcatacg gtaacaataa aatttcgaat atggataaaa tatttgatgt taaatttgat
      661 ttaaataagg aaaatagtaa tggattaggt ttatatattg taagtaatat tctgaataat
      721 tacaatatag aatataaagt attgcaggat gaagaatttt ttatctttaa aataaaaata
      781 ttttcttaat taagatataa ttttgcaata gttattgaaa aatagagact tat
//
LOCUS       DASJPC010000246          827 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.259, whole genome shotgun sequence.
ACCESSION   DASJPC010000246 DASJPC010000000
VERSION     DASJPC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.259"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..259
                     /locus_tag="U4A13_002950"
     CDS             <1..259
                     /locus_tag="U4A13_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN1781187.1"
                     /translation="EIHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMP
                     ESANDMYEKELKYFFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            260..>827
                     /locus_tag="U4A13_002951"
     CDS             260..>827
                     /locus_tag="U4A13_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN1781188.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFK"
BASE COUNT          319 a           97 c          145 g          266 t
ORIGIN      
        1 agaaattcat ttagattatt ttggaagagt tcctacaaga aaatttcaat tgtttacttt
       61 agatgaatgt atcaatgttg acttattaaa taataaaata agttttgaag aaaacattgt
      121 taaagaaatg cctgaatctg ctaatgatat gtatgagaag gagttgaaat atttctttga
      181 aaacgtagta acaggcaaag aaaactggaa tgatttatat catgcaaacg aagtattgaa
      241 aatagctgag gagaaataaa tggaaatttt atttactatt tgtggtagag ctgggtctaa
      301 ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag cccctagtcc atttcgcttt
      361 agcaatgata gatatctatc aacaaaaaca tcctgaacat aattgcacga cttgtttgaa
      421 tacggacagt aatgagttag tagaattgac gactaagtta aatccgtctg tgaaaattgt
      481 agaacgtaaa cctgaattag ctacagatac tgtagggaaa attgaagtga tacgtgattc
      541 attcttacaa ctagaaagag aagataatat atttgattta attatagatt tagatttgac
      601 ttcaccttta cgtgaagttt ctaatcttga agaattaata gatgaacatc taaaaaattt
      661 agataaagac gtctactttt cagttgtttc tgcaagacgt aatccctatt ttaatatggt
      721 aaaaagaatt gaaaatgaag ttactatagt taataaatca agatacacat ctaggcaaca
      781 gacacctgaa gtttttgaaa tgaatgcctc aatgtattct tttaaac
//
LOCUS       DASJPC010000247          811 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.260, whole genome shotgun sequence.
ACCESSION   DASJPC010000247 DASJPC010000000
VERSION     DASJPC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.260"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..672
                     /locus_tag="U4A13_002952"
     CDS             <1..672
                     /locus_tag="U4A13_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781189.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DASJPC010000248          803 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJPC010000248 DASJPC010000000
VERSION     DASJPC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.261"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            330..>803
                     /locus_tag="U4A13_002953"
     CDS             330..>803
                     /locus_tag="U4A13_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN1781190.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEK"
BASE COUNT          307 a           88 c          131 g          277 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aag
//
LOCUS       DASJPC010000249          754 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJPC010000249 DASJPC010000000
VERSION     DASJPC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.262"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(91..>754)
                     /locus_tag="U4A13_002954"
     CDS             complement(91..>754)
                     /locus_tag="U4A13_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEN1781191.1"
                     /translation="RRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQE
                     QPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSL
                     TDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQ
                     YQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGE
                     AA"
BASE COUNT          204 a          177 c          145 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gcgc
//
LOCUS       DASJPC010000250          746 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.263, whole genome shotgun sequence.
ACCESSION   DASJPC010000250 DASJPC010000000
VERSION     DASJPC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.263"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>746
                     /locus_tag="U4A13_002955"
     CDS             <1..>746
                     /locus_tag="U4A13_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781192.1"
                     /translation="GEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAI
                     AVLNYLKKRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMID
                     IYKEKKYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGF
                     IEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNP
                     SCKKNAYDIERLLKQKTMKNILKNVKIAES"
BASE COUNT          290 a           92 c          138 g          226 t
ORIGIN      
        1 ggagaggttt ggttggaagg aatatatgct tgcttatatc gaaataataa aaatgtacag
       61 gtatcaattt tagaagaagg tattttaagc tatggagttg acattattcc aacagcaaga
      121 acaaaagcta tcgcagtact caattactta aaaaaaagga ctccactata taacaaaagc
      181 tataattttt attgtttttt tcctaatata tatacaggaa gagagaatac aatacaaatt
      241 ccattagtta cagctgggtc aagaaccgca ttgcaacttt cggatatttt taaccctatg
      301 atagatattt ataaagaaaa aaaatatata ttttttacgt ctgtttatga ttttgagggc
      361 ggagaacctg tcggagaata tgaattagta tgcaaaatag cagacttagt aggaaaagat
      421 aatctaattg ttaagacgca tcctagagat gtaagaacaa tttataaaga taatggattt
      481 atcgaggata aaaattcatc aataccttgg gaggcgattc aattgtcagg cgattttaac
      541 gataaaatat ttatgacaat taattcagga agtgttattt caggtagtac aatgtctgca
      601 aaacctgtac gcacgtatta tatgtataaa ttgtgtaaca tagaaggaaa tccgtcttgc
      661 aaaaagaatg cgtatgatat tgagcgattg cttaagcaaa aaacgatgaa aaacatttta
      721 aagaatgtga aaatagcaga aagctt
//
LOCUS       DASJPC010000251          744 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.264, whole genome shotgun sequence.
ACCESSION   DASJPC010000251 DASJPC010000000
VERSION     DASJPC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.264"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(164..>744)
                     /locus_tag="U4A13_002956"
     CDS             complement(164..>744)
                     /locus_tag="U4A13_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781193.1"
                     /translation="DVVGTIKFFKHYNIIDIGDYSMEEKKIYKIEQNLELELLNESHY
                     EEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNLFSFTSEFYFLRHKERIVGMVS
                     IIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINEVSILEVKKLVIHLLNSTEDES
                     LIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
BASE COUNT          266 a          106 c           81 g          291 t
ORIGIN      
        1 tagttctaaa gccataaaaa gaattaattt ttgtaggagg attatttttc attagattag
       61 atacaactag taatacgaga gacaaaatca ataaaatcac ttcttttccc tcctatatct
      121 tatgatatga tccgaattat taaaatacta tcagaaattt tatttaataa atgtaagcgt
      181 atctcaaaac atcatgctct ctatattctt tagatagtac cgcttcttta aaaaaatgtg
      241 aatcttttaa ataggggatt agtgattcat cttctgttga atttagcaaa tgaatcacta
      301 gcttctttac ttctaaaatt gaaacttcgt taatattttc taatactgtt gtcaaaatag
      361 tcgtagtata tgcaggctca catatgaggt aatttactat actgtaggat aagttttgaa
      421 atggattaac aattatagaa accattccta caattctctc tttatgtctt aaaaagtaaa
      481 attcactcgt aaacgaaaat aaattatatc taaggacaac ttctaattct ttactatttc
      541 cttgatgata aatatcttca tgaaaataca tactttcagg atgatctgtg ataaaattat
      601 aaatttcttc ataatgtgat tcatttagta attctaattc taaattttgt tcaattttat
      661 atattttctt ttcttccata ctataatctc ctatatcaat aatattatag tgtttgaaaa
      721 atttaatagt tcctacaaca tctt
//
LOCUS       DASJPC010000252          738 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.265, whole genome shotgun sequence.
ACCESSION   DASJPC010000252 DASJPC010000000
VERSION     DASJPC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.265"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..709)
                     /locus_tag="U4A13_002957"
     CDS             complement(<1..709)
                     /locus_tag="U4A13_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN1781194.1"
                     /translation="MKFCIKRNVASHYLNQLFSDGKLLKNDSVRPVMFKYNQQKPKDC
                     FSKFIGADISLKSTIDKCKATVMYPPNGLPLIIKGNSGVGKSFLASLIYQYALDRKVI
                     HNDAKFVVVNCADYANNPELLSAVLFGYKKGAFTGAEKDTAGMLSSANGGYLFLDEVH
                     NLSAENQEKLFLLMDSQKYRKLGESVQWEYANVRLILATTEDKNTSLLATFRRRIPAE
                     ITLPDYNNRSTSEKIQLL"
BASE COUNT          233 a          132 c          115 g          258 t
ORIGIN      
        1 ataataattg aattttttca gatgtacttc tattattgta atctggcaga gtgatttcag
       61 ctgggatgcg tcttctaaaa gttgctaaca aagaggtatt tttatcttct gttgtggcta
      121 agataaggcg aacatttgca tactcccatt gcacactttc accaagttta cgatactttt
      181 gagaatccat aagaagaaac aatttctctt ggttttctgc acttagattg tgaacctcat
      241 ctaaaaaaag gtatcctcca ttagcagaag atagcatccc tgcagtatct ttttcagccc
      301 cagtaaaggc tcctttttta tatccaaata aaactgctga caatagctct ggattatttg
      361 cataatctgc acaattcaca acaacaaatt ttgcgtcatt atgaattact tttctatcaa
      421 gagcatactg atagatcaaa cttgctaaaa aactttttcc aacgccactg tttcctttta
      481 ttattaatgg gaggccatta gggggataca ttacagtagc tttgcattta tctatagtgg
      541 atttcaaaga tatgtctgct ccaataaact tagaaaagca gtcttttggc ttttgttgat
      601 tatacttgaa cattactggt ctaacagaat cattctttag taattttcca tctgaaaata
      661 actgattcaa atagtgggaa gcgacatttc ttttaataca aaatttcatt gcaatatctt
      721 gagcagaaaa atcgtaat
//
LOCUS       DASJPC010000253          736 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.266, whole genome shotgun sequence.
ACCESSION   DASJPC010000253 DASJPC010000000
VERSION     DASJPC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.266"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            34..657
                     /locus_tag="U4A13_002958"
     CDS             34..657
                     /locus_tag="U4A13_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781195.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
BASE COUNT          258 a          112 c          156 g          210 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaat
//
LOCUS       DASJPC010000254          733 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJPC010000254 DASJPC010000000
VERSION     DASJPC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.267"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>733
                     /locus_tag="U4A13_002959"
     rRNA            <1..>733
                     /locus_tag="U4A13_002959"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DASJPC010000255          716 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.268, whole genome shotgun sequence.
ACCESSION   DASJPC010000255 DASJPC010000000
VERSION     DASJPC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.268"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(203..>716)
                     /locus_tag="U4A13_002960"
     CDS             complement(203..>716)
                     /locus_tag="U4A13_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN1781196.1"
                     /translation="NNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSK
                     ECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKN
                     IEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDK
                     EILKVGNIDE"
BASE COUNT          250 a          115 c           83 g          268 t
ORIGIN      
        1 ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa acctcataca aaactataat
       61 tccaccccaa gcaaaagcta ttttcatcgg gatattagtt acattaaaag tactaggcat
      121 aaaataaagc agacaaatga ttaaatttat tactcgaaac gcatctcgac ttaataaact
      181 cttctctaaa acaggtacta ttctattcat ctatatttcc aactttcaaa atttctttat
      241 caattctatt taatagttta tcagagtaat ccgatttaac ttttcttaca aataattttt
      301 cactttcttt tatttgatct atatcccttt catcaaacca tttgttcatg gaaggatcaa
      361 taatatgaat atttgctaat ttcgaagttt gtaaagtagt ttcttcaata tttttcaatt
      421 caagtggtaa atctggtcct ccaataacat ttttattttt gaacgaggaa ttaaaaaaaa
      481 gtgtatgaaa aaatttttca tctggggcaa acatacctat tttaaaattc aggtagtctt
      541 gttgattttg ttcatacatt tgtattaatt ctgttaggca ttctttcgac aaagcccacc
      601 attgagaacc atgatacaaa tcaaatttat ggtgtctaat ttttggggga agtttaatca
      661 tcctcagaca acgattaatt atagctctaa aaattttaaa tgcagttgtg ttattc
//
LOCUS       DASJPC010000256          699 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJPC010000256 DASJPC010000000
VERSION     DASJPC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.269"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..433)
                     /locus_tag="U4A13_002961"
     CDS             complement(<1..433)
                     /locus_tag="U4A13_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1781197.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFF"
BASE COUNT          214 a          120 c           95 g          270 t
ORIGIN      
        1 caaaaaaaat ttgatatttt tttagatttc tttttagtct ttgtaatact ttataagcca
       61 cagaataact cacgtgatgc tgtttagcaa aattttctac tgagatatat ttttcctgaa
      121 agaaagccaa taccattttc atagataaag aatccgttat ataacgagat aacagagctt
      181 tgctagaaca ttttccatta atttccagac atatatccaa ttcactgacg gttactcgca
      241 aatttttctc tagatcgaac ttttgtaaat catgtttcag cttatcgatc gtttttttca
      301 agagaaatgt cgatagcgac aactcttctt gaacattcga tattgactct gataaatttg
      361 agcttgcttc taaatattta agtaatgaat aacttctgtg atcttctttg cttaaaaaaa
      421 actcataatt cattagtcat cagctctttc tacgtatatt actttccatg cattcaccaa
      481 ctagctgggg ataaaactga tttcctggat ccgagtcact gttgtcagcg attcttgttt
      541 gtataatata catcgtcctg tctttttttt aacattttgg agagcgtttt atttggcctt
      601 tctcgcgatc aaacctgtta ttagaattat atcatttact agtatgcatt tttattaata
      661 aaatggtata ttttatattg ttataaaaag agcccaacc
//
LOCUS       DASJPC010000257          620 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.270, whole genome shotgun sequence.
ACCESSION   DASJPC010000257 DASJPC010000000
VERSION     DASJPC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.270"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          222 a           99 c           98 g          201 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga
//
LOCUS       DASJPC010000258          606 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJPC010000258 DASJPC010000000
VERSION     DASJPC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.271"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..499
                     /locus_tag="U4A13_002962"
     CDS             <1..499
                     /locus_tag="U4A13_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HEN1781198.1"
                     /translation="YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIK
                     QPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEF
                     VYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCAN
                     ASEAV"
BASE COUNT          184 a          111 c          131 g          180 t
ORIGIN      
        1 ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc tgttcaatcg
       61 agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa aaaaagcatt
      121 tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc gtggaaaaga
      181 gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc gatcattgag
      241 tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt cgttgaaagt
      301 agaatttgtc tatcaataca catttgaaac cttacaacaa ttggatttgg agttatttga
      361 ctatgtcaat tggtggaacc accttcggtt gcacggtaca cttggctacg agacaccggt
      421 tggttaccgt aaccagagat tggcgcagcg aatccttgat aatgagctcg gatgtgctaa
      481 cgctagcgag gcagtctaac tttaactgtt agctcctgcc gaagatcgtc acatccgagg
      541 aggctcattg tcaaggacaa tcggaatagc atacggaaag aagttctgca ccttataaaa
      601 tttgtc
//
LOCUS       DASJPC010000259          600 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJPC010000259 DASJPC010000000
VERSION     DASJPC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.272"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          253 a           95 c           85 g          167 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
//
LOCUS       DASJPC010000260          565 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJPC010000260 DASJPC010000000
VERSION     DASJPC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.273"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>565
                     /locus_tag="U4A13_002963"
     CDS             <1..>565
                     /locus_tag="U4A13_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781199.1"
                     /translation="DEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKA
                     DPESGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIYDSQVAFQLVKQSKRLFP
                     EINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICP
                     QDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT          186 a          106 c          117 g          156 t
ORIGIN      
        1 gatgaagcga aagcctatca ggatgaatta gaagatgaga tcaatcgtca acgaatagag
       61 gctggaaaac gcccttttac tttggactta gaaaaggaag tgaagctgaa agaaagaaaa
      121 ataagtaaag ctgatccgga aagcggctac tacgtcaaag gcgaacgaga aaaacaattt
      181 gcttattctg cccatacttc atgcgacgac aatggattta ttcttagcac aatcatcact
      241 cctggaaata tttatgacag tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt
      301 tttcctgaaa tcaattgtgt tgtcgctgat gctgggtata agacacctaa gtttgttcac
      361 tttttgactc atttgaacct tcgaccttgt ttgccttatt caaggcccaa agggaaaaag
      421 gggctattat caaaaaatga gtttctctat gacgagtatt ttgattgtta tatttgtccg
      481 caagatcaaa tgctcgcttt ttcaacggtt actcgagaag ggtaccgaga atacaaatcc
      541 aatccaaaag aatgtgtgaa ctgtc
//
LOCUS       DASJPC010000261          564 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.274, whole genome shotgun sequence.
ACCESSION   DASJPC010000261 DASJPC010000000
VERSION     DASJPC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.274"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            141..398
                     /locus_tag="U4A13_002964"
     CDS             141..398
                     /locus_tag="U4A13_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781200.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          238 a           68 c           80 g          178 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaa
//
LOCUS       DASJPC010000262          546 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.275, whole genome shotgun sequence.
ACCESSION   DASJPC010000262 DASJPC010000000
VERSION     DASJPC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.275"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..75
                     /locus_tag="U4A13_002965"
     CDS             <1..75
                     /locus_tag="U4A13_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN1781201.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDDNE"
     gene            253..>546
                     /locus_tag="U4A13_002966"
     CDS             253..>546
                     /locus_tag="U4A13_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781202.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYG"
BASE COUNT          205 a           94 c          114 g          133 t
ORIGIN      
        1 ccagaaactg cgatccgtat ggaaattgat caactgaaca gacaacagag actttttggt
       61 gatgacaatg aatagagctg aagcgctaag aatagggacg gcaattgcta attgctggtg
      121 gaaatactat aaaccaatca tcctaagcca acaacatatt gacaagcaaa aagcatggca
      181 acaaataaaa aagtgactcc gccggcaagc aaagagtcac aaagaaaaca catcataagg
      241 agattttagc atatggaaaa agaactttcc actctagatc aatatttgat tgatcctgat
      301 tggggcaagc cgaaaattga ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg
      361 aatgaagaac tagcttggga tcaagatgat ttaggcaacc atgtaactat ttgggatcat
      421 gtttatctta tccatctatc gaagcattcg aataaacctg aatatattta cgtcatcgaa
      481 gatggcttga ttgatgcgct agaagagtac gacagagata acttgattga tatctcttat
      541 tacgga
//
LOCUS       DASJPC010000263          542 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.276, whole genome shotgun sequence.
ACCESSION   DASJPC010000263 DASJPC010000000
VERSION     DASJPC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.276"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..75
                     /locus_tag="U4A13_002967"
     CDS             <1..75
                     /locus_tag="U4A13_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN1781203.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            249..>542
                     /locus_tag="U4A13_002968"
     CDS             249..>542
                     /locus_tag="U4A13_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781204.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
BASE COUNT          213 a           94 c          105 g          130 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa aagcatggca
      181 acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc taaggagaat
      241 tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat tctgaatggg
      301 gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacgaacg
      361 aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg gatcatgttt
      421 accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc atcgaagatg
      481 gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc tcttattacg
      541 ga
//
LOCUS       DASJPC010000264          532 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJPC010000264 DASJPC010000000
VERSION     DASJPC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.277"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>532
                     /locus_tag="U4A13_002969"
     CDS             <1..>532
                     /locus_tag="U4A13_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781205.1"
                     /translation="IVDKMSFICADGETGKLIDILPTRKLSRLTHYFSRCANPEAVEY
                     LVTDMNAAYFQLTKRVLPNAKVVIDCFHIIKHLNQAFNDFRVQEMKRLHQLGQRREAE
                     KVKKNWRFLLKNRQKINHYDYKTWRSFRAPKYPLLTEAMMIDRLLAFSTPLKDAYTFF
                     HELTEAFRDKESDLFFT"
BASE COUNT          171 a          106 c          104 g          151 t
ORIGIN      
        1 attgtggata aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt
       61 ttgcctacac gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa
      121 gcggtggaat atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc
      181 ttaccaaatg cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg
      241 ttcaatgatt ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa
      301 gccgagaaag tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac
      361 tatgattaca aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca
      421 atgatgattg atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc
      481 catgagctga ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DASJPC010000265          528 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJPC010000265 DASJPC010000000
VERSION     DASJPC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.278"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(5..90)
                     /locus_tag="U4A13_002970"
     tRNA            complement(5..90)
                     /locus_tag="U4A13_002970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="U4A13_002971"
     tRNA            complement(107..178)
                     /locus_tag="U4A13_002971"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="U4A13_002972"
     tRNA            complement(190..262)
                     /locus_tag="U4A13_002972"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="U4A13_002973"
     tRNA            complement(280..361)
                     /locus_tag="U4A13_002973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="U4A13_002974"
     tRNA            complement(369..441)
                     /locus_tag="U4A13_002974"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="U4A13_002975"
     tRNA            complement(445..517)
                     /locus_tag="U4A13_002975"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DASJPC010000266          526 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJPC010000266 DASJPC010000000
VERSION     DASJPC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.279"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="U4A13_002976"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="U4A13_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN1781206.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="U4A13_002977"
     CDS             complement(349..>526)
                     /locus_tag="U4A13_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781207.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DASJPC010000267          514 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJPC010000267 DASJPC010000000
VERSION     DASJPC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.280"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(107..>514)
                     /locus_tag="U4A13_002978"
     CDS             complement(107..>514)
                     /locus_tag="U4A13_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEN1781208.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DASJPC010000268          514 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.281, whole genome shotgun sequence.
ACCESSION   DASJPC010000268 DASJPC010000000
VERSION     DASJPC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.281"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            5..>514
                     /locus_tag="U4A13_002979"
     CDS             5..>514
                     /locus_tag="U4A13_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781209.1"
                     /translation="MFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISEVTLDET
                     DDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGDNVLVDT
                     ILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHGYLIEIL
                     KKYEITVPRD"
BASE COUNT          190 a           64 c           90 g          170 t
ORIGIN      
        1 gaatatgttt acaaatgatc aatttaaatc tcttttaatt aaaacattag aaagaaagag
       61 ctcttcaaat tattatgaag gaggtaacga gattgtttct aaaatgaaaa tatcagaggt
      121 aactttagat gagactgacg attttactta ttataaagga tataaaaagg gctggaatac
      181 gctttgcaca tatcttaaaa taagagttcc ttttgatgat ttagactttt ttgaaagtca
      241 taaagatttg attacccaga cagcaagctc catatatgat aaacaaggtg ataatgtatt
      301 agtggatact attttagtac cacttccaga gaattacgaa gtaattaact ttagtcagtt
      361 gaaaattagt gatgtggttt cacaagctat tgaagacgct gagtctttta tgagtaatgg
      421 tgaatatcaa cgtgcctttg acagagttca cactgctttt catggatatt taattgaaat
      481 actaaaaaaa tatgaaatta cagttcctag agac
//
LOCUS       DASJPC010000269          514 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJPC010000269 DASJPC010000000
VERSION     DASJPC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.282"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U4A13_002980"
     CDS             complement(<1..132)
                     /locus_tag="U4A13_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN1781210.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DASJPC010000270          507 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.283, whole genome shotgun sequence.
ACCESSION   DASJPC010000270 DASJPC010000000
VERSION     DASJPC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.283"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          227 a           70 c           81 g          129 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttctatcgaa aaaaacttca aaaaagttct tgacaaataa
      361 caaacgataa gttatgataa atgagttgcc gaaagatttc gaaaaacgaa atcgaaaaaa
      421 acttttaaaa aagagttgac aatcgcttgt ctctctgata tgatataaaa gttgctacgg
      481 cagccaaaaa tgaatcggga aacactc
//
LOCUS       DASJPC010000271          503 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.284, whole genome shotgun sequence.
ACCESSION   DASJPC010000271 DASJPC010000000
VERSION     DASJPC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.284"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            10..375
                     /locus_tag="U4A13_002981"
     CDS             10..375
                     /locus_tag="U4A13_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1781211.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
BASE COUNT          201 a           35 c           86 g          181 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac att
//
LOCUS       DASJPC010000272          485 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.285, whole genome shotgun sequence.
ACCESSION   DASJPC010000272 DASJPC010000000
VERSION     DASJPC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.285"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..259)
                     /locus_tag="U4A13_002982"
     CDS             complement(62..259)
                     /locus_tag="U4A13_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1781212.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DASJPC010000273          473 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJPC010000273 DASJPC010000000
VERSION     DASJPC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.286"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>473
                     /locus_tag="U4A13_002983"
     CDS             <1..>473
                     /locus_tag="U4A13_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781213.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           71 c          112 g           98 t
ORIGIN      
        1 accgcaggag aaggaacaag tattgaaagt ggaacaacag tctttgcagt gaaagacggc
       61 gtaagcttac cagaagataa acttccagtg ttgaaagcaa aagatggtta tacagatgcg
      121 aaatggccag aggaagcaac tcaaccaatt acagcagatg atacagaatt tgtatcaagt
      181 gcaacaaaac tggatgataa atctgacgct gacaaatata atcctgaagg tcaaaaagtg
      241 actacagaat tgaataagga accagacgca tttgagggaa ttaagaacaa agaagattta
      301 ccaaaagata ctaagtatac ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa
      361 aaagggactg ttgtagtgac atatccagat ggatcatctg atgaagttga agtagatgtt
      421 acagtgacag acaatcgttc tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DASJPC010000274          462 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.287, whole genome shotgun sequence.
ACCESSION   DASJPC010000274 DASJPC010000000
VERSION     DASJPC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.287"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..430)
                     /locus_tag="U4A13_002984"
     CDS             complement(<1..430)
                     /locus_tag="U4A13_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1781214.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DASJPC010000275          460 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJPC010000275 DASJPC010000000
VERSION     DASJPC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.288"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..91)
                     /locus_tag="U4A13_002985"
     tRNA            complement(20..91)
                     /locus_tag="U4A13_002985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="U4A13_002986"
     tRNA            complement(113..200)
                     /locus_tag="U4A13_002986"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="U4A13_002987"
     tRNA            complement(225..298)
                     /locus_tag="U4A13_002987"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="U4A13_002988"
     tRNA            complement(312..382)
                     /locus_tag="U4A13_002988"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="U4A13_002989"
     tRNA            complement(384..459)
                     /locus_tag="U4A13_002989"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DASJPC010000276          459 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.289, whole genome shotgun sequence.
ACCESSION   DASJPC010000276 DASJPC010000000
VERSION     DASJPC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.289"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>459)
                     /locus_tag="U4A13_002990"
     CDS             complement(<1..>459)
                     /locus_tag="U4A13_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781215.1"
                     /translation="KIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRI
                     LPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVK
                     FLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELR"
BASE COUNT          143 a           85 c           87 g          144 t
ORIGIN      
        1 ttacgaagtt cattcatttc acggatacgc aactcattaa aggcttgatt catgtgtttg
       61 ataatatgaa accgatcaat cactactttc gcatttggca gaatacgttt ggtaagctgg
      121 aagtaggcgg cgttcatgtc tgtgactaaa aattttactt cttcaggatt agcgcagcct
      181 aagaaatagc ttgttaatcg aggcaattta cgcgtaggca agacatcaat taattgtcct
      241 gtttcgccat ctgcgcaaat aaagctcatt ttatcttcta tggaagcgtg cgaacgaaat
      301 tcatcaacca tcaatactct ggggagaatc ctcttagatt gctttggtaa gtagctttta
      361 aactctttca acgtacgaat aacggtggtc aaagatacct gacaattttt cgcaataaaa
      421 gataaagata ctttttcagt cagtaaagaa gcaatttta
//
LOCUS       DASJPC010000277          452 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJPC010000277 DASJPC010000000
VERSION     DASJPC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.290"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>452
                     /locus_tag="U4A13_002991"
     CDS             <1..>452
                     /locus_tag="U4A13_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781216.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DASJPC010000278          440 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJPC010000278 DASJPC010000000
VERSION     DASJPC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.291"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          135 a           87 c           52 g          166 t
ORIGIN      
        1 aattattacg ttattaaaaa aacagatgaa agatcaggtt ttattagatc tcattctttc
       61 atcacttgaa gggatttaat gaactacttt ttcctctttg tcgtttttat atattcgtat
      121 tccttcaaac agtaggatag cctctttcaa tgcttgatat ttgaactgtc cgttacttaa
      181 aaatttagat gtgccgccct cttcctagaa catattcttt tttacttttt cattaattga
      241 cagaccaatc gaacgaaact gtaaatttcg ataggataat gattggtcaa actcattcat
      301 tttcaagaca tctcgattca tattcaagat aggaatcttt ttatcaacat cactttattc
      361 gtattttcct ctaccataaa agcttcacgc tccacaaaat tgttttatac actactattc
      421 agcccattca acgaccaatg
//
LOCUS       DASJPC010000279          438 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.292, whole genome shotgun sequence.
ACCESSION   DASJPC010000279 DASJPC010000000
VERSION     DASJPC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.292"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            77..427
                     /locus_tag="U4A13_002992"
     CDS             77..427
                     /locus_tag="U4A13_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN1781217.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          180 a           47 c           71 g          140 t
ORIGIN      
        1 attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt tttggtagtt
       61 ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag tcatacatga
      121 attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag atattactga
      181 tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa ttcttgtaaa
      241 taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct ttattgataa
      301 tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa ttaatagtaa
      361 atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa caatgcctca
      421 acgataaaat atagtata
//
LOCUS       DASJPC010000280          415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.293, whole genome shotgun sequence.
ACCESSION   DASJPC010000280 DASJPC010000000
VERSION     DASJPC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.293"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..339)
                     /locus_tag="U4A13_002993"
     CDS             complement(<1..339)
                     /locus_tag="U4A13_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1781218.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DASJPC010000281          409 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.294, whole genome shotgun sequence.
ACCESSION   DASJPC010000281 DASJPC010000000
VERSION     DASJPC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.294"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..379
                     /locus_tag="U4A13_002994"
     CDS             <1..379
                     /locus_tag="U4A13_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEN1781219.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DASJPC010000282          402 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.295, whole genome shotgun sequence.
ACCESSION   DASJPC010000282 DASJPC010000000
VERSION     DASJPC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.295"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..390
                     /locus_tag="U4A13_002995"
     CDS             <1..390
                     /locus_tag="U4A13_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781220.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          156 a           72 c           61 g          113 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tg
//
LOCUS       DASJPC010000283          399 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.296, whole genome shotgun sequence.
ACCESSION   DASJPC010000283 DASJPC010000000
VERSION     DASJPC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.296"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DASJPC010000284          381 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.297, whole genome shotgun sequence.
ACCESSION   DASJPC010000284 DASJPC010000000
VERSION     DASJPC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.297"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(21..94)
                     /locus_tag="U4A13_002996"
     tRNA            complement(21..94)
                     /locus_tag="U4A13_002996"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="U4A13_002997"
     tRNA            complement(108..181)
                     /locus_tag="U4A13_002997"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="U4A13_002998"
     tRNA            complement(209..281)
                     /locus_tag="U4A13_002998"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="U4A13_002999"
     tRNA            complement(301..374)
                     /locus_tag="U4A13_002999"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DASJPC010000285          365 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.298, whole genome shotgun sequence.
ACCESSION   DASJPC010000285 DASJPC010000000
VERSION     DASJPC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.298"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          144 a           47 c           45 g          129 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcct
//
LOCUS       DASJPC010000286          361 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.299, whole genome shotgun sequence.
ACCESSION   DASJPC010000286 DASJPC010000000
VERSION     DASJPC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.299"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>361
                     /locus_tag="U4A13_003000"
     CDS             <1..>361
                     /locus_tag="U4A13_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781221.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAP"
BASE COUNT          127 a           76 c           72 g           86 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 a
//
LOCUS       DASJPC010000287          356 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.300, whole genome shotgun sequence.
ACCESSION   DASJPC010000287 DASJPC010000000
VERSION     DASJPC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.300"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..>356
                     /locus_tag="U4A13_003001"
     CDS             75..>356
                     /locus_tag="U4A13_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781222.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
BASE COUNT          126 a           66 c           64 g          100 t
ORIGIN      
        1 tgaaccccta tttcttgtgc gtttcttgtg gtattctgta acaaaatcag ttcgattcaa
       61 ggtggtgaaa aattttggaa actacagata cgttgcttca gttgcttcaa gaagcccata
      121 aaaccaatca agcacaacaa caaaccattc agaatcttac aactgaaatc caactattga
      181 atgaaaaggt caattattta accaacaaat tatttggacg ctctaaagag actctctttg
      241 aggaaacgaa tgggcaattg aatcttttta gtgatgaaga aatttctgta agtgttccag
      301 aagcagctgc caccattatt ccagtaaaag gacatcaacg agttgtagga acgaaa
//
LOCUS       DASJPC010000288          338 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.301, whole genome shotgun sequence.
ACCESSION   DASJPC010000288 DASJPC010000000
VERSION     DASJPC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.301"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..338
                     /locus_tag="U4A13_003002"
                     /pseudo
     CDS             1..338
                     /locus_tag="U4A13_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DASJPC010000289          334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.302, whole genome shotgun sequence.
ACCESSION   DASJPC010000289 DASJPC010000000
VERSION     DASJPC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.302"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..325
                     /locus_tag="U4A13_003003"
     CDS             <1..325
                     /locus_tag="U4A13_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEN1781223.1"
                     /translation="GNIIKIVLSASIPILIDQASEHRSLLLIVSIASAIITIIQSGMS
                     AFNYQDKVQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDF
                     NQVNN"
BASE COUNT          135 a           48 c           40 g          111 t
ORIGIN      
        1 cggaaatatt ataaaaatag tcttatcagc tagtatccca atattaattg atcaggcttc
       61 cgaacacagg tctttactat tgattgtttc aattgcttca gcaataatta caattataca
      121 aagtggtatg tctgcattta attatcaaga taaagtacag acttcgacag agttattaat
      181 gaaaattgaa aaagaaaagt tgttatatat aacgaaaaca tccccatata ataaaacaga
      241 tgaagaaaat ttccatttaa tcgttacaac ccttcaaact gaattaaatg atattatctc
      301 tgattttaat caagttaata actaaaaatt tatt
//
LOCUS       DASJPC010000290          331 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.303, whole genome shotgun sequence.
ACCESSION   DASJPC010000290 DASJPC010000000
VERSION     DASJPC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.303"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>331
                     /locus_tag="U4A13_003004"
     CDS             <1..>331
                     /locus_tag="U4A13_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEN1781224.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DASJPC010000291          323 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.304, whole genome shotgun sequence.
ACCESSION   DASJPC010000291 DASJPC010000000
VERSION     DASJPC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.304"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>323
                     /locus_tag="U4A13_003005"
     CDS             <1..>323
                     /locus_tag="U4A13_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1781225.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPK"
BASE COUNT          107 a           65 c           56 g           95 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaa
//
LOCUS       DASJPC010000292          323 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.305, whole genome shotgun sequence.
ACCESSION   DASJPC010000292 DASJPC010000000
VERSION     DASJPC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.305"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4A13_003006"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="U4A13_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN1781226.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DASJPC010000293          321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.306, whole genome shotgun sequence.
ACCESSION   DASJPC010000293 DASJPC010000000
VERSION     DASJPC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.306"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(69..321)
                     /locus_tag="U4A13_003007"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="U4A13_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DASJPC010000294          313 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.307, whole genome shotgun sequence.
ACCESSION   DASJPC010000294 DASJPC010000000
VERSION     DASJPC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.307"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..>313
                     /locus_tag="U4A13_003008"
     CDS             15..>313
                     /locus_tag="U4A13_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEN1781227.1"
                     /translation="MTTSLQVAESFEKKHQHVLRDIDALKKDVSNFGQMFVEGNEPDS
                     YGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIRKNTVPQTIE"
BASE COUNT          119 a           44 c           65 g           85 t
ORIGIN      
        1 aaaataaaga agctgtgact acgagcttac aagtggcaga aagcttcgag aaaaaacatc
       61 aacatgtttt aagagatatt gatgcactaa aaaaagatgt gtccaatttt ggacagatgt
      121 ttgtagaagg taatgaacca gattcatatg gcagaaatcg acgagttttc ttcattagta
      181 gagatggatt tttcttgctg gctatggggt ttacaggaaa gaaagctatc tacttcaaac
      241 aaaaatacat tgaagcattc aacgaaatgg aagatgttat tcgcaagaat actgttcctc
      301 aaacaattga aga
//
LOCUS       DASJPC010000295          311 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.308, whole genome shotgun sequence.
ACCESSION   DASJPC010000295 DASJPC010000000
VERSION     DASJPC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.308"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            92..208
                     /locus_tag="U4A13_003009"
     CDS             92..208
                     /locus_tag="U4A13_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEN1781228.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          140 a           34 c           37 g          100 t
ORIGIN      
        1 ctaattaaca aaagaatttg aattgttaat aaaatccaat aaaatatatt aataaagtag
       61 atagaaataa aaaaacaaag actaaacata tataattgga ttaaacgcaa tatctgcaaa
      121 atattgtaaa tttaaagcct ttgtagaaaa atttactgaa agacaaactg ataagattat
      181 tcagtatcat attacctata ctgattaaaa ttttttgaaa cgggatacta aataaaaaat
      241 ttatttataa cagaactaat tatttaatgc ggatgcgcca atacttggtc ctgcaagagc
      301 tatttatttt g
//
LOCUS       DASJPC010000296          309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.309, whole genome shotgun sequence.
ACCESSION   DASJPC010000296 DASJPC010000000
VERSION     DASJPC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.309"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..>309
                     /locus_tag="U4A13_003010"
     CDS             51..>309
                     /locus_tag="U4A13_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN1781229.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT          120 a           41 c           61 g           87 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggc
//
LOCUS       DASJPC010000297          308 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.310, whole genome shotgun sequence.
ACCESSION   DASJPC010000297 DASJPC010000000
VERSION     DASJPC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.310"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(76..>308)
                     /locus_tag="U4A13_003011"
     CDS             complement(76..>308)
                     /locus_tag="U4A13_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781230.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DASJPC010000298          297 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.311, whole genome shotgun sequence.
ACCESSION   DASJPC010000298 DASJPC010000000
VERSION     DASJPC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.311"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..297
                     /locus_tag="U4A13_003012"
                     /pseudo
     CDS             1..297
                     /locus_tag="U4A13_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          118 a           59 c           55 g           65 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatc
//
LOCUS       DASJPC010000299          297 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.312, whole genome shotgun sequence.
ACCESSION   DASJPC010000299 DASJPC010000000
VERSION     DASJPC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.312"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           85 a           70 c           50 g           92 t
ORIGIN      
        1 cgactttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
       61 cccaaccaaa cgaaaaatat gagcagctat ttttatttgg ctgttcttat tacttgatgc
      121 agggcagctt tttcacaacc tctccactaa acggtgttca atcagctaac cttcggtatt
      181 agctcaaaaa acggcaaaat atgaggaact attttgccgt ttgtttctct aatactcagg
      241 tcaaagcgct gatttcacaa cctctaatac acggtgttcc agaagtagct tcttcga
//
LOCUS       DASJPC010000300          286 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.313, whole genome shotgun sequence.
ACCESSION   DASJPC010000300 DASJPC010000000
VERSION     DASJPC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.313"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>286
                     /locus_tag="U4A13_003013"
     CDS             <1..>286
                     /locus_tag="U4A13_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN1781231.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSK"
BASE COUNT           90 a           57 c           49 g           90 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaaga
//
LOCUS       DASJPC010000301          279 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.314, whole genome shotgun sequence.
ACCESSION   DASJPC010000301 DASJPC010000000
VERSION     DASJPC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.314"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          101 a           50 c           25 g          103 t
ORIGIN      
        1 tttaactatt cactctaaca gttaaattat tttctcccat cacatgacta gaagcacatc
       61 ttctcaacaa actttttcaa aatgatcact catataatta caccttcatt aatttaaaca
      121 gcactacaaa ttccaataat taatgagcat atcgtatttg tatacaaaaa aatcgttgtt
      181 aaggcaaaaa tagccgtttt tacgctcatt ttatatactg ttggaaggtg ataatccaca
      241 ttactatagt ttacttttta gaaaatatta ttctttata
//
LOCUS       DASJPC010000302          270 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.315, whole genome shotgun sequence.
ACCESSION   DASJPC010000302 DASJPC010000000
VERSION     DASJPC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.315"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>270
                     /locus_tag="U4A13_003014"
     CDS             <1..>270
                     /locus_tag="U4A13_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN1781232.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DASJPC010000303          261 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.316, whole genome shotgun sequence.
ACCESSION   DASJPC010000303 DASJPC010000000
VERSION     DASJPC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..261
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.316"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            22..>261
                     /locus_tag="U4A13_003015"
     CDS             22..>261
                     /locus_tag="U4A13_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1781233.1"
                     /translation="MIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGKTALDYWH
                     QYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT           98 a           45 c           49 g           69 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt c
//
LOCUS       DASJPC010000304          256 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.317, whole genome shotgun sequence.
ACCESSION   DASJPC010000304 DASJPC010000000
VERSION     DASJPC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.317"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           86 a           47 c           35 g           88 t
ORIGIN      
        1 gtatagcaag caaaattctt ctccacttcc agaatttttt attttcattt ttagaaaagt
       61 gaaaaaactg acaacaaaag cttgtttctt tcactctatt aatatcttta gtgaaaaagc
      121 tcgttttata tcgtattctt ccagcgaatg tacttctcat aagtccaaaa aaagctgcga
      181 aaaagaaagt acctcggtac ctactttttc gtagctgcac ataacttgag caagtattaa
      241 tcgaagatac ttttgt
//
LOCUS       DASJPC010000305          252 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.318, whole genome shotgun sequence.
ACCESSION   DASJPC010000305 DASJPC010000000
VERSION     DASJPC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.318"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..>252)
                     /locus_tag="U4A13_003016"
     CDS             complement(32..>252)
                     /locus_tag="U4A13_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN1781234.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT           73 a           61 c           41 g           77 t
ORIGIN      
        1 aggctcatcg cagtaatagt aattttcttt ttcatttgtt tcctccttct tcgctttcag
       61 ccgagaacat tttgtaggtt cgatttgata cacccaacac actccctaaa aacgcgccaa
      121 aaccagtaat gatgacaaca cagatatctg tgtactgcca attgagcgct ttaccaacta
      181 accccacgaa agtagctagt gcgggaataa ttaccagtgc gaaccatttt agtacttcga
      241 acgttttatt at
//
LOCUS       DASJPC010000306          245 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.319, whole genome shotgun sequence.
ACCESSION   DASJPC010000306 DASJPC010000000
VERSION     DASJPC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.319"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>245
                     /locus_tag="U4A13_003017"
     CDS             <1..>245
                     /locus_tag="U4A13_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEN1781235.1"
                     /translation="EMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMEVLGGKESR
                     AYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAV"
BASE COUNT           97 a           31 c           53 g           64 t
ORIGIN      
        1 gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag atactatgcg aattagcgga
       61 caacaagagt ttgaaattaa gcaaaaagga aatatgaaag ttatggaagt tctaggggga
      121 aaagaaagcc gagcttatga agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa
      181 tttaaacgta ccttttcaat cccaagatat ggcgagttac ctcgtaagag attcgatgat
      241 gctgt
//
LOCUS       DASJPC010000307          239 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.320, whole genome shotgun sequence.
ACCESSION   DASJPC010000307 DASJPC010000000
VERSION     DASJPC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.320"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>239
                     /locus_tag="U4A13_003018"
     CDS             <1..>239
                     /locus_tag="U4A13_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN1781236.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DASJPC010000308          227 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.321, whole genome shotgun sequence.
ACCESSION   DASJPC010000308 DASJPC010000000
VERSION     DASJPC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.321"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>227)
                     /locus_tag="U4A13_003019"
     rRNA            complement(<1..>227)
                     /locus_tag="U4A13_003019"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DASJPC010000309          224 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.322, whole genome shotgun sequence.
ACCESSION   DASJPC010000309 DASJPC010000000
VERSION     DASJPC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.322"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           91 a           36 c           28 g           69 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aatt
//
LOCUS       DASJPC010000310          224 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.323, whole genome shotgun sequence.
ACCESSION   DASJPC010000310 DASJPC010000000
VERSION     DASJPC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.323"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>224
                     /locus_tag="U4A13_003020"
     CDS             <1..>224
                     /locus_tag="U4A13_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN1781237.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DASJPC010000311          214 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.324, whole genome shotgun sequence.
ACCESSION   DASJPC010000311 DASJPC010000000
VERSION     DASJPC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.324"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..149
                     /locus_tag="U4A13_003021"
     CDS             <1..149
                     /locus_tag="U4A13_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781238.1"
                     /translation="AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT           65 a           46 c           43 g           60 t
ORIGIN      
        1 atgcctttaa ggtgtcccac tcaaacggcg tcactgaggg gctgaataac aagatcaagc
       61 tgattaaacg aattgcgttt ggttatcgca acttttataa ctttcgggca cggatctatt
      121 tacaacaagg tctgatcttt gaaaactaaa aaaatatgcg gcgcacagaa cgcgccgcat
      181 atcggactaa gttttattca aatttgtctt cacc
//
LOCUS       DASJPC010000312          209 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.325, whole genome shotgun sequence.
ACCESSION   DASJPC010000312 DASJPC010000000
VERSION     DASJPC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.325"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT           64 a           38 c           39 g           68 t
ORIGIN      
        1 caactaaaaa aacatttttg aaaataaaca tcagcagtcc tatcaatgca tgtgagctta
       61 tagaagttat tcgcagcgca gcataattgc attagcttct tttcgtgttt tttgcctctt
      121 ttccctttaa atcttaatgg aggaggtgtg caatatgtca gataacgacg aacgcgtaaa
      181 tgcccagttg attcgattct ttgaaaagg
//
LOCUS       DASJPC010000313          206 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.326, whole genome shotgun sequence.
ACCESSION   DASJPC010000313 DASJPC010000000
VERSION     DASJPC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.326"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>206)
                     /locus_tag="U4A13_003022"
     CDS             complement(<1..>206)
                     /locus_tag="U4A13_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781239.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DASJPC010000314          205 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.denovo.327, whole genome shotgun sequence.
ACCESSION   DASJPC010000314 DASJPC010000000
VERSION     DASJPC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.denovo.327"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>205
                     /locus_tag="U4A13_003023"
     CDS             <1..>205
                     /locus_tag="U4A13_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781240.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DASJPC010000315         6361 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.119, whole genome shotgun sequence.
ACCESSION   DASJPC010000315 DASJPC010000000
VERSION     DASJPC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.119"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1077
                     /locus_tag="U4A13_003024"
     CDS             <1..1077
                     /locus_tag="U4A13_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN1781241.1"
                     /translation="SFELNQAVLNGWEDRKGRKINVPIADGGENTAEAIYYALRGTWR
                     TIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKPTDQTIRLASSYALGE
                     LIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEGNPLLSTKKLHFDRRN
                     VLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLDKQAGKIAQQIKDFWE
                     VDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKIEQADVIITGEGRIDQ
                     QTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIVLSIHLGPIGLEEAME
                     HRQTLEKTKILGQTLSRFFTILPK"
     gene            1335..2837
                     /gene="eat(A)"
                     /locus_tag="U4A13_003025"
     CDS             1335..2837
                     /gene="eat(A)"
                     /locus_tag="U4A13_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN1781242.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2865..3431
                     /locus_tag="U4A13_003026"
     CDS             2865..3431
                     /locus_tag="U4A13_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN1781243.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            3642..4703
                     /locus_tag="U4A13_003027"
     CDS             3642..4703
                     /locus_tag="U4A13_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN1781244.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4706..5602
                     /gene="murQ"
                     /locus_tag="U4A13_003028"
     CDS             4706..5602
                     /gene="murQ"
                     /locus_tag="U4A13_003028"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN1781245.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5616..>6361
                     /locus_tag="U4A13_003029"
     CDS             5616..>6361
                     /locus_tag="U4A13_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HEN1781246.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILG"
BASE COUNT         2188 a         1097 c         1436 g         1640 t
ORIGIN      
        1 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
       61 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcagg
      121 ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat
      181 ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca agcattgggg
      241 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
      301 ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
      361 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
      421 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
      481 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
      541 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
      601 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
      661 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
      721 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
      781 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
      841 ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
      901 atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc aattgtctta
      961 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     1021 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     1081 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     1141 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     1201 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     1261 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     1321 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     1381 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     1441 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     1501 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     1561 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     1621 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     1681 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     1741 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     1801 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     1861 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     1921 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     1981 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     2041 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     2101 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     2161 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     2221 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     2281 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     2341 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     2401 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac
     2461 atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     2521 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     2581 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     2641 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     2701 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     2761 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     2821 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     2881 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     2941 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     3001 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     3061 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     3121 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     3181 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     3241 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     3301 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     3361 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     3421 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     3481 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     3541 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     3601 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     3661 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     3721 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     3781 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     3841 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     3901 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     3961 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     4021 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     4081 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     4141 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     4201 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     4261 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
     4321 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     4381 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     4441 atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     4501 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     4561 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     4621 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     4681 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
     4741 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     4801 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     4861 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     4921 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     4981 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     5041 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     5101 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     5161 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     5221 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     5281 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     5341 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     5401 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
     5461 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
     5521 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
     5581 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
     5641 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
     5701 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
     5761 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
     5821 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
     5881 taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca tctggaaaag
     5941 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
     6001 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
     6061 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
     6121 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
     6181 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
     6241 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
     6301 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
     6361 g
//
LOCUS       DASJPC010000316         6317 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.120, whole genome shotgun sequence.
ACCESSION   DASJPC010000316 DASJPC010000000
VERSION     DASJPC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.120"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            9..2456
                     /locus_tag="U4A13_003030"
     CDS             9..2456
                     /locus_tag="U4A13_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN1781247.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2686..3753
                     /gene="rlmN"
                     /locus_tag="U4A13_003031"
     CDS             2686..3753
                     /gene="rlmN"
                     /locus_tag="U4A13_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN1781248.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3946..5049)
                     /locus_tag="U4A13_003032"
     CDS             complement(3946..5049)
                     /locus_tag="U4A13_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN1781249.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5602..6150
                     /gene="aac(6')"
                     /locus_tag="U4A13_003033"
     CDS             5602..6150
                     /gene="aac(6')"
                     /locus_tag="U4A13_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN1781250.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2102 a         1088 c         1362 g         1765 t
ORIGIN      
        1 gataataaat ggcacaaaag aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga
       61 aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg tgttgggttt gccttgattt
      121 tatttgctgt cttcggcttt ttaaaattag gttttttagg aatattgatt gcaaacggct
      181 ttcggatcat tgcaggaaat acttaccaaa tcctctgtct tttattagcc gtattaggat
      241 tttggattgt cattaagaat acagagtttt caattggtaa aaaccgacgc tggtttggcg
      301 gcatactctt ttatttcggt atcttattat tgttacatgc ccatttattt ggcaaactcc
      361 atactgggga accaaatatc atggggacga catgggattt gttagccagt gatataaaac
      421 aaagtcaagt agacaataac gttggtggtg ggatgattgg agcaatcctt tatcatttta
      481 cttacttctt gattgcacag cctggaagtt atttagcagc agtgctatta ctggcaggag
      541 gagcattcct gttcagtaat ctggaaggct atcagctgtt aaatggtatc caatcaattg
      601 gagaacgagt acaggaatta ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg
      661 caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa
      721 aagaagctgc agaaaaagaa gctgcagaat atgagaagaa aaaagcggtg agccagcaaa
      781 gggaaaatac tgctgatgaa tggcaggaac cagaaaatac agagccggag cagctaagtt
      841 ttgttccaat cgacagtttt caagaaaata tccatcctgc gaatctagag aaaccagtac
      901 cagatacacc aaaacagacc aatacagcag aaggtttttc agatgagttg ccagaggatg
      961 acggtacctc attagaattt gagattgaag cagaacagga aaatcaagac tatgaattac
     1021 cgacagttga tttgcttgat tctataccaa ctgtggatca aagtgatgag tataaaaaaa
     1081 ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg
     1141 tcgtcaaagc cagcttagga ccatccgtca caaaattcga agtccagccg gctgtagggg
     1201 taaaagtaag taaaattgtg aacttaacag atgatatcgc gcttgcttta gcggcgaaag
     1261 atgtccggat ggaagcaccg attccaggta aatcactgat tggtattgaa gtaccaaatg
     1321 gaaagatcag tatggtttcc tttagagaaa tcatcgaagc acaaccaaac cacccagata
     1381 aattactaga agtaccttta ggacgtgatg tttctggacg ggtacaaacg gctgatcttt
     1441 cgaagatgcc tcacttactc gtggctggtt cgactggtag tgggaagtca gtagcaatta
     1501 atgggattat cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga
     1561 tcgatccgaa aatggttgaa ttgaatatgt acaacggcat acctcatctg ttgacgccgg
     1621 tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag
     1681 aacgatatga aaaattcgcg gcaaccggag taagaaatat ttctggttac aatgaatttg
     1741 ttcaacagaa aaacttggaa aacggaacga aacatccaac cttaccattc atcgtagtga
     1801 tcgtagatga attagctgat ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta
     1861 ttcgtttagc gcaaatggct cgggcagcgg ggattcatat gatcctagct actcaacgtc
     1921 caagtgtgga tgttatcaca ggaatcatca aagcaaatgt accttccaga atggcatttg
     1981 cggtatcaag cggtacagat tctcggacga tcattgattc taatggggca gaaaagctct
     2041 tagggcgagg agatatgcta ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag
     2101 gagcattcat ttctgaccac gaagtagaac gggtcgttca atttgttaca gatcagcaag
     2161 aagctcatta tgaagagaaa atgatgccga cagatgaagt tgaaacgaca ggtgctccag
     2221 aacagccaca agatgagctg tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg
     2281 caagtatttc ccttctccaa agaagatttc ggatcggtta caaccgagca gcacgtttag
     2341 tagatgaact ggaagctcat ggagttgttg gcccgtcaga aggaagcaag cctcggaaag
     2401 tattcattga gcaggaagaa acatcagaaa cgttgccgga cgataatgac atgtaaaact
     2461 tgtgtgatga agggttgaaa aacgaataac caataaaaag acaaggcatg atgataccaa
     2521 aagaaaaaat ggtttttttc catcttttct aatacggtat gatgtcttgt ctttttagtt
     2581 tttgtgagat taaagttcta ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc
     2641 atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa atactttgga aaaacagtcc
     2701 atttatggct tgacgaatga agaattaatc aattggttta tagaaaacgg cgagaaaaaa
     2761 ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa
     2821 atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat catcaatcct
     2881 ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt gtttgaactc
     2941 cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt
     3001 gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa
     3061 aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat
     3121 tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag
     3181 ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga
     3241 ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag
     3301 gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat
     3361 gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa
     3421 gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg
     3481 aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag
     3541 aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat
     3601 tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta
     3661 aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga
     3721 agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac
     3781 cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc
     3841 tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt
     3901 cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt
     3961 atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt
     4021 aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt
     4081 atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg
     4141 aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat
     4201 catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt
     4261 ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt
     4321 aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc
     4381 atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg
     4441 atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc
     4501 acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa
     4561 gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt
     4621 tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt
     4681 gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc
     4741 accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc
     4801 tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc
     4861 gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc
     4921 tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac
     4981 gattccattt gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact
     5041 ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat
     5101 cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat
     5161 aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa
     5221 acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg
     5281 cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta
     5341 tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa
     5401 acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt
     5461 tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt
     5521 ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag
     5581 caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg
     5641 aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg
     5701 gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa
     5761 gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg
     5821 catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat
     5881 tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat
     5941 ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg
     6001 gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa
     6061 atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa
     6121 acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc
     6181 ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga aattccaact
     6241 tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta
     6301 gtttcgagta gtaagaa
//
LOCUS       DASJPC010000317         5613 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.135, whole genome shotgun sequence.
ACCESSION   DASJPC010000317 DASJPC010000000
VERSION     DASJPC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.135"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..1026)
                     /locus_tag="U4A13_003034"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="U4A13_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="U4A13_003035"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="U4A13_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEN1781251.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="U4A13_003036"
     CDS             1851..3005
                     /locus_tag="U4A13_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN1781252.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="U4A13_003037"
     CDS             3220..4188
                     /locus_tag="U4A13_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEN1781253.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="U4A13_003038"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="U4A13_003038"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEN1781254.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="U4A13_003039"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="U4A13_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DASJPC010000318         5548 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.137, whole genome shotgun sequence.
ACCESSION   DASJPC010000318 DASJPC010000000
VERSION     DASJPC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.137"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..233
                     /locus_tag="U4A13_003040"
     CDS             <1..233
                     /locus_tag="U4A13_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781255.1"
                     /translation="GYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEEL
                     LAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            240..617
                     /locus_tag="U4A13_003041"
     CDS             240..617
                     /locus_tag="U4A13_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN1781256.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            771..1553
                     /locus_tag="U4A13_003042"
     CDS             771..1553
                     /locus_tag="U4A13_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEN1781257.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1679..2410)
                     /gene="erm(A)"
                     /locus_tag="U4A13_003043"
     CDS             complement(1679..2410)
                     /gene="erm(A)"
                     /locus_tag="U4A13_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEN1781258.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2468..2527)
                     /locus_tag="U4A13_003044"
     CDS             complement(2468..2527)
                     /locus_tag="U4A13_003044"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEN1781259.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            2919..3581
                     /locus_tag="U4A13_003045"
     CDS             2919..3581
                     /locus_tag="U4A13_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN1781260.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3816..3959
                     /locus_tag="U4A13_003046"
                     /pseudo
     CDS             3816..3959
                     /locus_tag="U4A13_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            4109..4309
                     /locus_tag="U4A13_003047"
     CDS             4109..4309
                     /locus_tag="U4A13_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN1781261.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4563..>5548)
                     /locus_tag="U4A13_003048"
     CDS             complement(4563..>5548)
                     /locus_tag="U4A13_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN1781262.1"
                     /translation="ASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         1847 a          889 c         1068 g         1744 t
ORIGIN      
        1 atggttattg tagattgcca gtagtagcag gtggttgtcc acatgcgaat gcatgcttag
       61 attgcactca cttctgtacc agtaagcaat tcttaccaca gcacgaagaa cagttagagc
      121 gtacagaaga gttattagcc atagctaagg ataaacaatg gcaaagacaa gtagagacta
      181 atagccgtgt taaagagcgt ttagaacaaa tcattggaag tttgacgggg taattatcaa
      241 tggataaaca agttagaaat acaacagaaa ttgtacgttt ggcgaagcag aaatcaaaaa
      301 agacaaggga aaaagtagac aaagcgattt ctaaattttc gattgaaggt aaagttatta
      361 attttaattc aatagcaaag gaagctaatg tttctaaatc atggctttat aaggaacacg
      421 atattaggca aagaatcgaa tcccttcgtg agcgtcaaat aacagcaaat gtagtctcaa
      481 aacccaagaa aagttctcgt tcggaggaaa tccttattaa aaccttaaaa agaagagtaa
      541 tggaattaga aaaagaaaat aaaaaattac agaaccaaat tcaaaaatta tatggagatc
      601 tgtataataa agaataatta ttaatctgta gacaaattgt gaaaggatgt acttaaacgc
      661 taacggtcag ctttattgaa cagtcattta ggtatatgtc caatctaggg taagtaaatt
      721 gagtatcaat ataaacttta tatgaacata atcagttacg aggtgaaatc atgagcaatt
      781 tgattaacgg aaaaatacca aatcaagcga ttcaaacatt aaaaatcgta aaagatttat
      841 ttggaagttc aatagttgga gtatatctat ttggttcagc agtaaatggt ggtttacgca
      901 ttaacagcga tgtagatgtt ctagtcgtcg tgaatcatag tttacctcaa ttaactcgaa
      961 aaaaactaac agaaagacta atgactatat caggaaagat tggaaatacg gattctgtta
     1021 gaccacttga agttacggtt ataaatagga gtgaagttgt cccttggcaa tatcctccaa
     1081 aaagagaatt tatatacggt gagtggctca ggggtgaatt tgagaatgga caaattcagg
     1141 aaccaagcta tgatcctgat ttggctattg ttttagcaca agcaagaaag aatagtattt
     1201 ctctatttgg tcctgattct tcaagtatac ttgtctccgt acctttgaca gatattcgaa
     1261 gagcaattaa ggattctttg ccagaactaa ttgaggggat aaaaggtgat gagcgtaatg
     1321 taattttaac cctagctcga atgtggcaaa cagtgactac tggtgaaatt acctcgaaag
     1381 atgtcgctgc agaatgggct atacctcttt tacctaaaga gcatgtaact ttactggata
     1441 tagctagaaa aggctatcgg ggagagtgtg atgataagtg ggaaggacta tattcaaagg
     1501 tgaaagcact cgttaagtat atgaaaaatt ctatagaaac ttctctcaat taggctaatt
     1561 ttattgcaat aacaggtgct tacttttaaa actactgatt tattgataaa tattgaacaa
     1621 tttttgggaa gaataaagcg tcctcttgtg aaattagaga acgctttatt actttaattt
     1681 agtgaaacaa tttgtaacta ttgaaaatag aaagaaattg ttccttcgat agtttattaa
     1741 tattagtgac atttgcatgc ttcaaagcct gtcggaattg gtttttagtg aaaagaacac
     1801 gatattcacg gtttacccac ttataaacaa aagatcgata ctttttgtag tccttctttg
     1861 aaatcaatgg ttgatgtcgt tcaagaacaa tcaatacaga gtctacactt ggcttaggat
     1921 gaaaatatag tggtggtact tttttgagca tttttatatc catctccacc attaatagta
     1981 aacccaaagc tcgttgcaga ttttgcaatc ttttcgcaaa tcccttctca acgataagat
     2041 agctatattt agcctgactt tcaaaggtaa ttcttttgac aatatccgta ctgatgttat
     2101 aaggaatatt accatatatc ttatagttta tatgttttgg gaaggaaaat tttagaatat
     2161 ccgtttgaat cacttttata ttctcagagg ggtttaccgc ttctttagtc acttgacata
     2221 agcctccatc aatttctata gcagtaactg atcgactcat tttgactagc tctttggtaa
     2281 aatgtccttt tcctgatccg atttctatta cgttgtcttg tttactgata ttcgtgtgat
     2341 tcaatatttc ttttacatgc tttttagaag taataaaatt ttgcgtgtct ttagggtttt
     2401 tctggttcat tataaccttc tccttactgg ttataatgaa ctgacattaa gcgttcatta
     2461 taaccaatta attttgattt ggttgataac gaactttatt aataacaaat atagaaaaag
     2521 tacccatact tttatctcct ttccattttt ttcattatga atgagataaa gtaatttcta
     2581 ctactgcgat actggtgcac ataattgaaa cctccttatg taactgaata tgattgtatc
     2641 atcttttcat tttagaataa acctttatat gtaattttgc acttgacact tcagatggac
     2701 gttttcttca aaataatatg ttttgaaact tgataagaga agccactgtc tttactaaaa
     2761 agtaaaggct gcgataaagg acagtgactt taataaagtt gcactgttgt attgtctttg
     2821 gataaaattg tctaaaggga agttttatcc ccaacgaaaa aacatctaaa gtggtaagta
     2881 atgaaagaat gtcgataaag taggaatggg ggatcttttt gaatacaata gaatcacagt
     2941 ttgataaggt tgcagaagat tacgatttcg tgaatgagct tttgaatgat tattcattct
     3001 ttgtgtctaa tatgtctcca aagaaaggca gagcattaga tatcggatgt ggctcgggtt
     3061 tgttagtgga gaaattagca agttattatg atgaagtggt agggattgat atttctaatc
     3121 aaatgctcga tcttgccaaa tctaaacgtc aactaacaaa tacggtctat ctgaatatga
     3181 atgcagaaca acttaatttt aatgagaagt ttgattttat tgtaagccga acaacctttc
     3241 atcatttgga tgatatagcc agcgtaatac agcaaatgaa ggaactgttg aatgaggaag
     3301 gaagaatagt tattcttgat aatgtatctg aagtcgagac accgcctacc tatgtttata
     3361 aattaggggc aatccaagag tttttaccgc attgtttcaa atttggaata aaaaatgcga
     3421 ttagaattta taatcacaac acatctaaat catggcttga gcatttagct tcagataagt
     3481 acctatctga acaaaattat tacgatttat atgaaaagtt gttgcctgga tgccaattcc
     3541 ataaaatggg ttgggcaatg ggggttgtct ggacaaaata acttaaagct gtggctttga
     3601 aaagttgaat ttgtgattgt tttcagagct atcgggtgct ttagtataaa taacaatgaa
     3661 taaaaaatca gcctgtatct tatgttacta ttaagtaatt taagatacag gttttactat
     3721 ttttgaaata agtaccttcc acttcaaata catgtattca aagtgaaagt attgatttaa
     3781 atgattcttt tgtaatttac tcttgataat atgaaattcc atcgcagcag gatatgaatt
     3841 ttaccaaaag gatacaaaag tgtggagaaa tgatggggat cactgtactg gaccacttga
     3901 tcatcggacg caaacgatat tttagtttga gagaagaagg aatgatggaa gagaagtaag
     3961 ttgctttctt gcaaaaaaaa tgaagcaagt gtacaattat attgtaattt tgtttgggaa
     4021 tagaagttga aacatctgtt gcacctctat cgtacacgag caatgttaca atttaaatta
     4081 gtgcttttgg cacgaggagg atattattat ggaaaacgga acagtaaaat ggttcaacgc
     4141 tgaaaaagga tttggattta tctcacgtga agatggaagc gacgtattcg ttcacttctc
     4201 agctatccaa ggtgacggat tcaaaacatt agaagaaggt caagcagtga ctttcgacgt
     4261 tgaagaatca gaccgcggcc cacaagctgc aaacgttgta aaagcttaat tttatctgta
     4321 aatcaaattg atggtagaca tcaattttta cgagagaaca aagcgagtaa caaaaatacc
     4381 tgccacaaga gacaatttct cttgtggcag gtattttttt gtatgaaaaa gtaaataaat
     4441 ttaagaggct atgaaagagc caccttgtat caaggaataa gaacaaacaa aaatggcttc
     4501 tcatgttttt catttgtttt ctcttatatt caggacaagg tgctgatttc ataacctctt
     4561 atttattctt catctggtgt cattaccatt ggcaaaatca ttggatgtct ttctgtacgt
     4621 tcgaataaga aaggctgaag cgcgccagtc atcgcttctt tcaatttata ttctgaacag
     4681 tcaggtccat tcaaacattc tcttaaagcg tggaataaga ttcgctgagc ttcattgatc
     4741 atttctcctg attctcgcat gtagacaaat ccacgagaga gaatatctgg accagccaag
     4801 atttcttttt tcttgatatc cactgtcgcg acagctaata caagaccttc ttctgaaagc
     4861 aggtgacggt cacgtaaaac gacattacca atatcaccga tcccgctacc atcgatatac
     4921 acatctccag ccacaaaatg cccagcagga cgcgcagaat cgctagttaa cgctaaaaca
     4981 tcgccatttt ccatgatgaa gcagttttct gcaggaacac ctacatcttg tgctagagaa
     5041 gcatgtactt tcaacatgcg gaattctccg tggacaggaa taaaatattt tggcttcatc
     5101 aaacgaagca ttaatttttg ttcttcttgt ccaccgtgac cggaagtatg gatgttattg
     5161 atttttccgt gaatcacttc tgcaccagct tcagacagca aattgatcaa atgattgaca
     5221 cttgttgtat taccaggaat aggtgaactt gaaaagatca ctgtatcgtc tggctggatt
     5281 tggatttgac ggtgagtacc attcgcgatg cggctcaacg cggccatcgc ttctccttga
     5341 gaacctgtac ataggatcat cgtttcattg gcaggcaagc gattgatttc gtgtgcatct
     5401 acaaatgtgc cgtctggaac tttgatatat cctaaacgtt gaccgttgac gatggcattt
     5461 tccatgcttc gtccgaaaac agcgatttta cgaccagttg ccacagcggc atctgctgct
     5521 tgctgcaaac ggaagatgtt agaagcaa
//
LOCUS       DASJPC010000319         4986 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.146, whole genome shotgun sequence.
ACCESSION   DASJPC010000319 DASJPC010000000
VERSION     DASJPC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.146"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            109..1149
                     /locus_tag="U4A13_003049"
     CDS             109..1149
                     /locus_tag="U4A13_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN1781263.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1247..1435
                     /locus_tag="U4A13_003050"
     CDS             1247..1435
                     /locus_tag="U4A13_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEN1781264.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1493..1846)
                     /locus_tag="U4A13_003051"
     CDS             complement(1493..1846)
                     /locus_tag="U4A13_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1781265.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2377..2799
                     /locus_tag="U4A13_003052"
     CDS             2377..2799
                     /locus_tag="U4A13_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN1781266.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2796..3026
                     /locus_tag="U4A13_003053"
     CDS             2796..3026
                     /locus_tag="U4A13_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN1781267.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3524..3724
                     /locus_tag="U4A13_003054"
     CDS             3524..3724
                     /locus_tag="U4A13_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN1781268.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3751..4944
                     /locus_tag="U4A13_003055"
     CDS             3751..4944
                     /locus_tag="U4A13_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN1781269.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1716 a          807 c          916 g         1547 t
ORIGIN      
        1 actccaaaac tgaactactt acttgcatat atacgccttt atagtggagt actacattta
       61 cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca
      121 ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa
      181 aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa
      241 aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag
      301 agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat
      361 tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa
      421 gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca
      481 gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg
      541 ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga
      601 atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcatttca aaatgcagtt
      661 atggaaggga tacgctatgg ttgcgaacaa ggattatatg gttggaatgt gacggattgt
      721 aaaatctgtt ttaagtacgg tttatactat agccctgtta gtactccagc agattttcgg
      781 atgcttactc ctattgtact ggagcaagcc tttagaaaag ctggaacaga attgttagag
      841 ccatatctta gttttaaagt ttatgcacca caggaatatc tttcacgggc atataacgat
      901 gctcccaaat attgtgcaaa tatcgtaaat actcaactga aaaataatga ggtcattatt
      961 attggagaaa ttcctgctcg atgtattcaa gattatcgca atgatttaac tttttttaca
     1021 aatgggctta gtgtttgttt agcagagcta aaaggatatc aggttaccac tggcgaacct
     1081 gtttgccaga cccgtcgtct aaatagtcgg atagataaag taagatatat gttcaataaa
     1141 ataacttagt gcgttttatg ttgttatata aatatggttt cttattaaat aagatgaaat
     1201 attctttaat atagatttga attaaagtgg aaaggaggag attgttatta taaactacaa
     1261 gtggatattg tgtcctattt gtggaaataa aacaagacta cgaatacgag tggatactat
     1321 acttaaaaat ttccctttat actgccccaa atgtaagaac gaaactttaa ttaatgttca
     1381 aaaaatgaat ataataacaa tcaaagagcc agacgccaag acgcagagcc gataatttga
     1441 gaaatgaaac tctcatctta tcggctcttt ttgtttatct gaattttact gactagcctt
     1501 caatatttct tgcttcattg ataccttttg ctagtgcttc cattaaggat agttctttgt
     1561 ctgtaaagct atccatgtat ttttctacct gtaaccttcg agtgctttta atcaaattat
     1621 ctgtaggtaa gaaaaattca tcaatagata catggagtaa tgatacaaga tcatacagca
     1681 cttgtgtact tgggtgttgt cctttgtttt ctatatttgt taaataacgt gggtcaattt
     1741 caatcattgt tcccacttgt tcacgagtga gaccccgttt cattcgagcc tctttaatcg
     1801 ctaaaccaac ggctctaaaa tcgtatttat cttctttttt acgcatacta aatcacctct
     1861 atacatttta ctgttcctat ataaccataa acaggtatag aaaaacatgt tatatagttt
     1921 atgagttcct atagaacact gtggaattta ttaatgcgat gaatccaatt tcaaatagaa
     1981 aggtcgctga ttttatttac ctatacaact ttataaaatt tagtcatact cctagaaagt
     2041 agataaaatt ggcgacttaa tggagggaaa agtcgccatg agaaagataa tctgtcatct
     2101 agttctctta attaagttct tatcaaaaaa acttatccac ctgtactgga tagatatgac
     2161 catcaatgtg gttttgcaga ttacatagat agaaaaatca aagcatatga acagagaaaa
     2221 caatctgttt atgtgctttt ttggtcactt aataaatatc ttttaggatt tctcttcatt
     2281 tatgcaacaa atcccccgtt cgtgtcgggg tttagtatga agggagataa agaagcacat
     2341 gccactccct ttcacgacag aaagggggtg aaaagcatga aaccatcttc ttttcaagaa
     2401 accatagaaa atcagtttga ctatatctgc aaacaagtta ttgaagatga acgaaaagat
     2461 tacttcaaac atctagacag actatcagca aaagaagtct cgttttctga tatagataac
     2521 taccttgtca actctttttc tactgttgat acgtacgtca ctgatttcca actatttaaa
     2581 ttatttggat taagaatcaa tgttgaaagt gatttattaa gtgaagccct gcgtcatctt
     2641 tcagagaaaa aacggaacat tattttactt cattacttta tggatatgag tgatgtggag
     2701 attgcggagt tactaaattt gaatcgttcg actgtttatc gtcatcgaat aagtggacta
     2761 gccatgatta aagaattcat gaaggagtgt aaagaatgaa aacagcttat ccaagggttc
     2821 ccttccccct cattgtaaag gcgacagatg gcgacgtgga agcaattaat cagattgtta
     2881 aacattatcg agggtatacc tcaaaacgtt cattacgtcg catgacagac gaatatggaa
     2941 attctcatat ggttattgat gaaaccctac gtggcagaat ggaaacgaaa ctcatctcta
     3001 agattttatc gttcgaaatt aaatagtccc attttttcgt ggaagcgtgt cgaatcgtat
     3061 ccacgcttcc cgatttggga ggtttgttat tccatcaaag catattgtgt gttcagtagg
     3121 ttttgatagg ctaacaagcc aaacgttctt tgacaaccaa ataaaggcaa tcgaatacgt
     3181 ttcggtaaga aaagagctga tgaactaaca cgccatgacc tacaaaatta gtgagcgatt
     3241 catgttagta attcgaaagt aatctttagc aggattgctg ccatgacttt cttatcgtga
     3301 taatgatact cccgtatagc catagtccga gcgttagaag cgtcacaggc aatgggtacg
     3361 gctacatgag aaccatgcag aagtgaaatt cctgtgagct ttgctagagc tgttcgattg
     3421 ctaagatatt cttctcttgt cagtgtatta ttataaaact atttgatata attataggtg
     3481 aatattggag ttttcctagc ctataattgg gaaaggagta ataatgataa cggtcacaaa
     3541 aaaggattta gttgagctag gatatggaac ttctttttca gcagacatca taaaaaaagc
     3601 aaaagaattg atgattacaa aagggcatat ctactatcaa tctcgaaagc tagatagagt
     3661 ccctcgtgaa gctgttgaag aaattctagg aattcggttt tcaaatgaaa acaggtctga
     3721 gtagtttttg cacagtaagg agtaaaaact atggttaaag acccaataaa aaaagcaaag
     3781 aatggaacct attattttcg tgcaaatcta ggttatgatt ctaaagggaa aaaaattcaa
     3841 aaatatcgta gtggattcac tactaaaaaa gaagcaagag aagaatattc gaaactgcta
     3901 ttgatgaaac cagaagaatt aagtgagaat aaggataaaa tgacgtttaa acattatatc
     3961 tttgaaattt tccttccatg gtataagaca caggtaaagc tcagaacata tgaaaatcgc
     4021 ttaccaacta ttaagaagca tttctcttat tttgataaaa tggctgtttc cgatattgag
     4081 cctattgatg tgcaaaattg gcaactaaaa ttatcaaaga agtgtaagtc ttcttatgta
     4141 agagctgttc aaggactatt ttcagttgct atggatcgag caattgtcct tgggataacc
     4201 accacaaatc catctaagat tattggcaat gtgaaaaagc aaaagtctaa aattgaattt
     4261 tggacaaaag aagaatttga aaaagtaatc tctctaattt acaaagaaga ttactatcaa
     4321 cactttttat ttatctctct gtggttttta tttatgactg gaatgcgaat tggtgaagct
     4381 actgccattc aatgggaaga tattgatttt gactcaggag tactaaccat caacaaaaca
     4441 ctctattaca agaatcaatc gaattataga tttgttgaac caaaaacaaa ggcgagcgtt
     4501 cgtcatattg tattagataa atgcacatta acttatttat ccgaatggaa ggaagtacaa
     4561 caaaatctaa ttcagacaga ctttgtaatg agctataacg gtattcctac ccaaaaacat
     4621 acactatcat atgctattga acgctatgct aagctggctg gtatccatcg aatcagaatc
     4681 catgggttac ggcattctca tgcttccttg ttaatcagta tgggagaaaa tccacttatc
     4741 attaaagatc gtctaggaca cgaagatatt gagacgactt taggaacgta tggacatctt
     4801 tatcctaaca gtaatttcga ggtagctaac aaattaaatg gagccatttc ctttaaagaa
     4861 gcaaaacaaa attatgatac ttcaccaaag aatcaattta ctgtagacta cttgcgaaac
     4921 aaagaaatga aaaaagtgca ataaaagtgg ctctataatt tatgggactg atgaatcaga
     4981 attgat
//
LOCUS       DASJPC010000320         3979 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.162, whole genome shotgun sequence.
ACCESSION   DASJPC010000320 DASJPC010000000
VERSION     DASJPC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.162"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..454
                     /locus_tag="U4A13_003056"
     CDS             <1..454
                     /locus_tag="U4A13_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN1781270.1"
                     /translation="AKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAYE
                     SGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPIG
                     GFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            808..2286
                     /gene="msr(C)"
                     /locus_tag="U4A13_003057"
     CDS             808..2286
                     /gene="msr(C)"
                     /locus_tag="U4A13_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN1781271.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2333..2779
                     /locus_tag="U4A13_003058"
     CDS             2333..2779
                     /locus_tag="U4A13_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN1781272.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2978..3493)
                     /locus_tag="U4A13_003059"
     CDS             complement(2978..3493)
                     /locus_tag="U4A13_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN1781273.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3610..>3979
                     /locus_tag="U4A13_003060"
     CDS             3610..>3979
                     /locus_tag="U4A13_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN1781274.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVG"
BASE COUNT         1263 a          662 c          849 g         1205 t
ORIGIN      
        1 agcaaaagaa atcttgcgtc ctttgatccg tttcaatacg agacaaaatg atacttcttc
       61 tgcaatggtc atgatttttg gcgatatgca atgttacgaa tatggcgaag aaatctacaa
      121 ccaagcttac gaatcaggga agatgccaaa agaagtcaga gatcaacaat tagctgcgat
      181 cattccttat tacaagagtt tttcaagaga agaaatgaac gatgtagtca aagtcgattc
      241 tagcttagcg gctatgcaat tcatgttagt tgcgcgtgat catggctatg aaacaaatcc
      301 aattggtgga ttcgaagcag atcagttggc agaggcattt ggtttggata aagaccgtta
      361 tgttccagtt attattttat caataggaaa agcggtagaa gaaggttacg agtctgttcg
      421 tttagcacca gaaaagatca ctatgttcga ataaaaggtt ttcagttact attcagtagc
      481 tattgctttt tttgaatcgt gtgtggtaac ctaataaaaa aggaggtcgg aaaaatgact
      541 gcatcgatga aattacgttt cgaacttttg aataacaact aaataaattc aaaagacatg
      601 tataaacaac aatttcatgg atggagtatg tccatttttt cgaacgacag tagaataggt
      661 gaaagctatg acagatttgg tgatttagtc atagttattt cgttcagagt aggaaaagat
      721 gtgatgcagt ttttttgcaa acagattttt ctgctctttt ttagtatcga taattgttgt
      781 tttacatgaa ataggagtgt aaaacatatg gaaaatttag cagtaaatat aacaaatcta
      841 caagtcagct ttggcaacca gctagaacta tctattgatt ctcttcgtgt ctatcagcaa
      901 gatcgtatag ggatcatagg agaaaatggt gtcggtaaat cgactttgct caaactaata
      961 gccggtgaac tttttcccga tcatgggaaa atccaaacag agatcacctt caactacctg
     1021 cctcaattaa cctatctcgc tgaagcaaag gacctaaatt tggaattagc cagtcatttc
     1081 cagttaagac tggaagaaac ttcagagcgg aaatggagcg gaggggaaga acgaaagatc
     1141 gagttgatac gtcttctttc ttcttatgaa caagggatgc ttctagacga gccaacaacc
     1201 catctagata gaaaaagtat tgatcgactg attgaagagc ttcgttatta ttatggtacg
     1261 ctggtttttg ttagtcatga tcgctatttt ctagatgagc tagcatcgaa aatctgggaa
     1321 gtaaaagacg gagaaatccg agagttttcg gggaattata gtgcctatct cactcaaaag
     1381 gaattggaga aaaagactca gctacgagaa gcagagtcga tcatgaaaga gaaaaaacga
     1441 ttggaaaaat cgatccaaga aaagaaaaaa caagcggaaa agttggaaaa agtgtccagt
     1501 aaaaagaaaa agcaacaaat cagactggat cggttgtctt cctctaaaca aaaagacagt
     1561 gtacaaaaag ccatccaaaa gaatgcgaaa acattagaga gaagactcca aaaaatagga
     1621 gaaacaacca agccgcagca gatgaaacaa atccgttttc cagtaccaaa atctcttgaa
     1681 ctccacagcc gctatcccat catgggacaa aatgtccaat tggaaagaag tgggagaaca
     1741 ttactggtaa atggtgattt tcagttttct ttaggtaaaa aaatcgcgat tgtcggtgaa
     1801 aatggttcag gtaagacaac cttattagaa catatccgca aacaaggaga aggaatcctt
     1861 ctctctccga aagtaagctt tcaagtatat cagcaaaagg gttatcaaat gacatctgaa
     1921 gaatccatca ttcgttttgt catgagacaa acagagtttt cagaatcgct tgtccgtagt
     1981 ttgctgaatc acttagggtt tgctcaggaa actttgacga aaccgttatg tacattaagt
     2041 gggggagaag cgacccgtct gacgattgct ttgcttttta ctaagccaag taatgtgttg
     2101 ctgttagatg aaccgactaa ttttattgat atggcaacga tcgaagcttt agagaagcta
     2161 atgcaaatat atccgggaac gattttgttt acttctcatg attcttactt tgtcgagcgt
     2221 acggctgatg aagtttatga aataaaaggg cagaaaataa aaaaagtact tacgagaaat
     2281 ttttaaatgt gctacgatga ttttcgtgac aaatttaaag gagtggtaca gattgcctac
     2341 aaataaaaaa gaaggaataa tttttacaac aatgatgtgt tttttgatgg tcttggggat
     2401 gagcgcgtat aatttatggc tgcatcaaga tcttcattta tcaagtttgt tgattggtct
     2461 agtgcctgga ttcgtggtgg ctttcatttt ggatgtcttc atcatcggtg tgctggcgaa
     2521 aaaaattgcc tttaaactac cagtaaatcc agaaagcaag gtccaaatga tcttaactat
     2581 ttcatgcttg atggttttag gaatggtgac atgtatgtct ttattcggtg tgatcatgga
     2641 acaaggaata cctgatcaat tattctctgc ttatatgaat gcttggaaaa tgaatgtgat
     2701 tgccgcttta ccgttgcagt tgatcatcgt tggcccgctt tcaagaagtg tgctaaaagc
     2761 aattcaaagt aatgaatgat ggaacactgt tatttgaaaa aaggccttgg catgcttttt
     2821 gtcatagtct cttgaatgca gttctagaaa aatagtgatc cgaacgatag gttttggcct
     2881 gtcgttcgga tcgctttttc ttttccaact gtattgctct tttattgaag ataatttgct
     2941 cttcaataga tatttctaga aaggttctgt ttatccctta ctcaaaaaac tgctgcattt
     3001 gttctcttac ttgctgacac cattggagtt cctgctgaca aagcataatt ctgcgctgga
     3061 tcacaacata acgtccaaat tcttgtttct tgtctagcga gaggtcattc aatttttttt
     3121 gatttttttg catgattttg ttcagctgtt gttctctagt gaatagcagt ccagtagctg
     3181 ttgttttatc gagcgtcgaa aaagcataga tttttgctag aaattcatct ttttgcgtag
     3241 gagcgggagt ttcctctttg atccattcct ctacaagagc acgtccttca tcagtcaagt
     3301 ggtagatctt tttttgagag tgctcgtctt tgtccaaaac ggtcacataa ccctgctggt
     3361 ctaacttggc aagcgttgta tagatctgac tgtgatgggc ttcccaaaaa attgtgatca
     3421 gctgcttcaa ttcataccca gaacgtggtt tggataaaag cacgcataat aatatatagg
     3481 ctaaagtatt cataaaaatc ctacctctcg tcttattttc tttattatat cacagtaaag
     3541 tttccacgtt ttacatgtgc atattgacat gtaaaataaa tcatgtatga tgctaataga
     3601 gagaaaggga tggaaatatt ggagaatcaa atagatatac aagaacaaac aaatgaagaa
     3661 acaaagggga aaagttcagc gctagttgta gcagctacct gtatgatcgc ttttatggga
     3721 gtgggtctag tagacccaat cttaaaaacg attgctgcac agttaaacgc tacgccagct
     3781 gaaacgaccc tgttatttac tagttacatg ttagtcactg gtgtggtgat gctatttaca
     3841 ggtttcttat ctagtcggat cggtttgaaa aaaacattga tgacaggatt gtttatcatc
     3901 gttgtattcg ctggtcttgg cggattatct gggaatatcg gaatgctgat tgggttacgt
     3961 gccggctggg gtgtcggga
//
LOCUS       DASJPC010000321         3937 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.164, whole genome shotgun sequence.
ACCESSION   DASJPC010000321 DASJPC010000000
VERSION     DASJPC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.164"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(60..1277)
                     /locus_tag="U4A13_003061"
     CDS             complement(60..1277)
                     /locus_tag="U4A13_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN1781275.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(1426..1605)
                     /locus_tag="U4A13_003062"
     CDS             complement(1426..1605)
                     /locus_tag="U4A13_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781276.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            1660..1743
                     /locus_tag="U4A13_003063"
     tRNA            1660..1743
                     /locus_tag="U4A13_003063"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1694..1696,aa:Leu,seq:gag)
     gene            complement(1983..3353)
                     /locus_tag="U4A13_003064"
     CDS             complement(1983..3353)
                     /locus_tag="U4A13_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781277.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
BASE COUNT         1311 a          764 c          597 g         1265 t
ORIGIN      
        1 aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg aaaaatttgg tttttaaaat
       61 catttttggc gataggttgc taggaattct ctcattttct tagctgtttg tttcaaatca
      121 tccattttag gaagataaac gattcgaaaa tgatctggct ggttccaatt aaatccacca
      181 ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca aaacgaattt ttcatcatcc
      241 aaaatattga atcgctttgt atcaattttt gggaatatat aaaaagcagc ttttggtttt
      301 acggctgaaa gaccaggaat atcattgatt gcattataga tatattctct ttgttcatag
      361 atccttccgc ctggcaggag cagctcatcg acactttgat aaccgcctag cgcggtttgg
      421 ataatttgct gagacaatac attggaacac aaacgcatgg aggacaacat attcagccct
      481 tctatatagc ctttgacgtg tgacttatct ccacttagga ccatccagcc tactcgaaat
      541 cctgctacac gatgagattt ggatagaccg ttcagcgtaa caacgaacaa atcaggcgct
      601 aatgtagcaa tcggaatatg ttctagacca tccatcacta agcgatcata gatttcgtcg
      661 gaataaataa tcaggttatt ttgtcgagct acttcaacga tttcttctaa taattctttt
      721 ggataaagtg ccccagtagg attattagga ttaatgataa caatagcttt tgtccgactc
      781 gttatttttg atttgatatc atcaatatca ggataccatt ctgcctcttc atcgcaaata
      841 taatgaacag gtgtgcctcc agctaatgca atagaagccg tccacaacgg atagtccggc
      901 attggaacca gtacttcatc tccattgtcc agcaacccct gcatacacat cgtgatcaat
      961 tcgctcactc cgttgccggt gtagatatca ttgatcgtca cattcggaaa atgtttcagt
     1021 tggcaatatt gctcaattgc ttttctagca gaaaagatcc ccttggaatc tgagtagcct
     1081 tctgagtcac gggcattcat gatcaaatca cgaatgactt cgtttggtgc ttcaaatcca
     1141 aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa tgccctcttc ttgcatccgc
     1201 tccgcttctt ctagaacggg accgcgaaca tcgtaactga caccttctaa tttgttggat
     1261 ttttcaaatt ctctcatcac ttagccaatc cctcatttca aaaagttaga actttccaaa
     1321 ttttttgtta tttactattt caatatcata ctccatccta gaaaattttg ccacttattt
     1381 aggaaaaacg taagtattaa aaaaacacaa acaagcgttg agaacttaat tgtcatgata
     1441 gtcattccat agattcgctg cccaatccat tgcaattcca caaatgattc ctagcataaa
     1501 accaagcaaa aaacgtccga atgcaagtcc aatcaataca cctaagatca ttcccagtcc
     1561 tgaaaattct ccgtcatatt tctccatctt aataagtcct cccatttttg agcttgacaa
     1621 ctatgtaaaa cctcgctatc attagtgtgt tattattacg cagatgtgtt ggaattggca
     1681 gacaagcatg attgagggtc atgtgagcgt tagctcgtgt gggttcgagt cccatcatct
     1741 gcactacaac tcgtgaagtt aattttgctt cacgagtttt tttgtttgaa cgtttcttta
     1801 tgccattata tacatatcac tgtatccttt acaaaaagaa cgtacaatca tgtaaacagc
     1861 ctgaggtgag tatatgaagg tcggaaacaa gctactcgta gaaacttgca agtgttaaaa
     1921 caattaatcg aaaaacgagt aaaatccctc acctaatact aaagcgaggg attttttgta
     1981 tattatctgt ttatcaccgt tattataaag gtacaaatac caagcacggt taatccaaat
     2041 tgcataaatt catttttttg tacactcatg cttttcatta gctctttaac tgccattagt
     2101 actaagatgg cgataacaac ataaagtata tagtacgtcc gcattccgga agcccatact
     2161 gtgggagaaa atcccataat taaccttgaa caaaatccca ttatcatcaa aataactaaa
     2221 aaaatagaag ttttcttatt gtcaaaactt aaataaattc caacaattaa acatagtaat
     2281 aatgccaaga ctactaaagt agctatccaa gtacctggat aaaaaagtga cagtttcgtt
     2341 cctaactctg taaagagatt attcaggtta ctactactcc aaatcattgc ccttttattt
     2401 ccattgacgt aagtaaagct ttgacccatt gtattaccaa aataaataat caggtttaag
     2461 aaaaacggta atgcagtcaa tatacgcaca taatagtttt gacatttcct atatgttaga
     2521 aggaaaatta agaaaaatag caataaaaat aagatattca tgtctaaaaa aaacggcttt
     2581 ccaaatgatg aaatacctaa atctaactta ttaagaaagt tgaaattttt atattcagga
     2641 aaccatttat taatttcatg tgcagcacgt aatgcattcc ctggagttgt caaagagaaa
     2701 attaattctg ctaagcttat tattgaaaat actgataact tataattata tcgtccattg
     2761 acaatcaaat ataaactaac tatacttgtt agaacaaaaa aacaggcgtt gacttgttct
     2821 tgattagtta aaaatatcaa taaaggaata cttacccaat atatttttct atttatttct
     2881 tctcctctta agagttgaaa aaacggataa aaagttagta aactaaatgc tactggccac
     2941 tgatagttca aggtggttgc tctccaaccg gtttccccca ttactgtcaa tggtattaaa
     3001 cagtatatag agcaaaccaa taataaattt ttaacattta ttttctcaaa aacatacttg
     3061 caaagtaata ctacaattcc taacataatc gtagaattta ataatttcca tagcataaag
     3121 tgtttagaaa agaacattaa gaatagttct ataataattc ttgaactcca tgtcttataa
     3181 tgttctatcg aagcacccaa atagccaagt tcatctaatc tacttgcata aactacgtca
     3241 tccgctctca tagtcggcat atacatatta aagagtaaaa agatacccca aatccctatt
     3301 agtaacaaga tcagttcttt gttattcgtt atttttctat tatgtttgtt catttttata
     3361 ctccttctta tatttcctaa tagagtataa catagcttta agcattactt aaagataact
     3421 taaaagttat cttttaaata cttaattgtt aaatacctta tttgatgcaa aagaaatcta
     3481 cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat ttttcttagt taaggaccta
     3541 tggattgcag taatgcagaa ttcgtcgaat aaactgccaa agacatacaa tcatattgct
     3601 tttaaaattg acgaacaaga tatagattcg tttgtctcaa aaatccaaat gttaggttta
     3661 actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt cttcaattta cttttacgac
     3721 tatgataatc acctcttcga gttacatacc ggtactctcc aggaacgttt aaaatcctac
     3781 aattccacta cctgagacta gtaatgacta gtctcttttt atggcctctg atccccccat
     3841 tgagcgaata ctaatagggc ttcgaattga tctgttcaat ctttaagcaa tatccctcaa
     3901 atgagtttgg aaagtttagt gattcatcac aaatact
//
LOCUS       DASJPC010000322         3480 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.171, whole genome shotgun sequence.
ACCESSION   DASJPC010000322 DASJPC010000000
VERSION     DASJPC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.171"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            294..791
                     /gene="dfrG"
                     /locus_tag="U4A13_003065"
     CDS             294..791
                     /gene="dfrG"
                     /locus_tag="U4A13_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEN1781278.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(863..2815)
                     /locus_tag="U4A13_003066"
     CDS             complement(863..2815)
                     /locus_tag="U4A13_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN1781279.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2822..3058)
                     /locus_tag="U4A13_003067"
     CDS             complement(2822..3058)
                     /locus_tag="U4A13_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN1781280.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(3293..>3480)
                     /locus_tag="U4A13_003068"
     CDS             complement(3293..>3480)
                     /locus_tag="U4A13_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN1781281.1"
                     /translation="RFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELC
                     GNVFKIEYTKKRQRLAY"
BASE COUNT         1085 a          597 c          571 g         1227 t
ORIGIN      
        1 aaaggcgtat atatgcaagt aagtagttca gttttggagt acaaaatgac aaaatgtact
       61 tcaaaatgga ctactttttt cattttaact tacataccaa attacataca aaatgattta
      121 taaaatgcaa ttgaaaatga taattgaatg aaaatttgtg attttgtggt attatgataa
      181 tataacttat agaaaaaatt gcttattcaa ctaacgggca ggataatgag aaaaggtttt
      241 ttgatagaaa taagaaatta gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag
      301 tttctttgat tgctgcgatg gataagaata gagtgattgg caaagagaat gacattcctt
      361 ggaggattcc caaggactgg gaatatgtta aaaatactac aaagggacat ccgataatat
      421 taggtaggaa gaaccttgaa tcaatcggaa gagccttacc tgacagaaga aatattattc
      481 tgacgagaga taaggggttt acctttaatg gttgtgaaat tgttcattca atagaagatg
      541 tttttgagtt atgtaaaaac gaagaagaaa tttttatttt cggaggagaa cagatttata
      601 atttgttttt cccttatgtt gagaaaatgt acatcacaaa aatacatcat gaattcgaag
      661 gagatacttt ttttccagaa gtgaattatg aggaatggaa tgaggtattt gcccaaaaag
      721 ggataaagaa tgataaaaat ccgtataact actattttca tgtatatgaa agaaaaaact
      781 tattgagtta acgggtgcgt taattgaaga agaacagtaa agataaagca caagagcttg
      841 ggattactcc aagctctttt tgtcatttga aataaattat atctttccaa tcaaattgtt
      901 tatctgtaat ccctaatctt tgagcaggtg tcaaaacctt tttatccgct gatttaaaag
      961 ggcgacaaaa gttgtagtaa gttcttagaa ttgtaatcgc aaattgagca tacttcggat
     1021 tgaagttagc gtaaatatag ctcttcttat caccacgagc tgttgttaat ggtctttcaa
     1081 gagatgatat ataacgtctt atctgttgaa taaagctatt ggtcgaatgg tcattaacat
     1141 ttaatagcat ctttgcgatt tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa
     1201 ctgaataaaa tcctttatcc ttagtggcta atggatgttt gatagggtta tcggcatatt
     1261 tgcgatatct ttctccatct ttactaatag cctcttcgtg gaattgatga cttgttagta
     1321 attcagttag ataatgagat gccaaagtcc gtagaaactt tgttttgaat cctctgatat
     1381 ctccccaatc gagcagttct gcttttgctt gtccgaattc ttttaagcat tgttttctgc
     1441 ttttcgtctt gttaacttgg caaataaaat gatgtgcatc gctcaacctt aattctttcg
     1501 tgaatactct ataaaaggcg tttctaatag agaagtcatc atcactaatc attcgccatt
     1561 cgtcggcatt aaccatttgt ttaattaacc aatagtgagc agttgtagta taaggagcat
     1621 caacatttaa accatcgaca aatctactcc tattagtaat tttccctaat tcgtgaagat
     1681 aagcattcct attttcgcta tcgttagcgg atggttcttg tggataataa gaccaatcta
     1741 acctatcatt tttacgacta aaagtattga gatggtcttc tttgaatttt cttgtatctt
     1801 catttaattc atccattgaa atattccaat cataggcaac atcagaacgg aacacatatc
     1861 ttgataagac ctcagcagat acaactatat acgtttgcat attaaggtct tcaaaccccg
     1921 tatatttctt tgaaccttgt cctttcttac ggacgttatt taggtagtag tgcatcttgt
     1981 ccgtattaag ccacatctcg ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt
     2041 ctaaaaactc caaacaacgc ctatacagcc actccagctt atgatagtag gttccaactc
     2101 caattcctaa aatatcacaa gtacgactga ctgaaaccct tccaacaacc atcttagcga
     2161 acattggaag aatggtgttc ttttgttggt gataagtagt ggtttcttct ctttttggta
     2221 gaacattagt gaacttctta cacgccttac attgatagcg ttgagatttg cccctacttt
     2281 tccccctttt gtaaaattgc ttcggctcat tgaatggggt tgattcttct tccgagcagc
     2341 cttctttatg aaattgataa tctggctcaa catcttggat gctattgatt tctataaggc
     2401 gtttaatttc ttcaacaaca gaccaattag ataaaggggt tactgtacag ttttgcgata
     2461 cacctctgcc aataggattg tcattgcagt acataccttt atgaccttta tctttggaac
     2521 taccagtcat tttatatcga cttggtttcc cttttacatc tttgaatttg tgctgttctt
     2581 tcccaaaatt acaacaaaac ggattgatgc agtaattata ttgaatggga tattcagaac
     2641 cgttccattt aaaagacgtt ggcaagaaca acattttgtt ataccttgtt gcaaatcgtt
     2701 ttggagttaa taaatcatat tgtttagccc tatcttttaa ttcttcgttt gaaatcggaa
     2761 ttttaacatc tacaatttca tcttccttcg tggctaatct tttaagcttg gtcattgtct
     2821 atcaacccac tctatctttg atacccattt tttcaacaat ccgcttatta cttcgcttgt
     2881 tggcaatcca ttcaataaac ttcttatcgt ctaaaatgtt caataagaat tcatccactg
     2941 cttcgctttc agtacgctct gcatattcag cgtattgctt aactatcacc cttacttgct
     3001 ctgatatttc ccaatccaca gacttagcgt tcttattttt tggctcaaga aattgcatag
     3061 gaaaatgcac ccctttttgc ttagtaaatt gtaaccattt taacacacca atttgcattc
     3121 ctacaataat tatttcacgt tcgcattttt atatacattt taacttacat tttgtggtgt
     3181 aaattaacaa caaacgttga tttaacaata aaaaacgagt atcaaattca gttgcaattt
     3241 gatactcatt taggtttaca ttttcacttt ttgtactcca aaactgaact acttaatatg
     3301 caagacgttg tctttttttt gtatattcaa ttttgaaaac atttccgcaa agttcagacg
     3361 gacctcgatg tgttgatgaa taaaatttat tagtaataac ttctataagg ttatcaatcc
     3421 ctatattact ttttgcactc ccatgataaa gagggaacag agaacaattc tgaaatctta
//
LOCUS       DASJPC010000323         1781 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.209, whole genome shotgun sequence.
ACCESSION   DASJPC010000323 DASJPC010000000
VERSION     DASJPC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.209"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            24..416
                     /locus_tag="U4A13_003069"
     CDS             24..416
                     /locus_tag="U4A13_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN1781282.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="U4A13_003070"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="U4A13_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HEN1781283.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DASJPC010000324         1504 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.218, whole genome shotgun sequence.
ACCESSION   DASJPC010000324 DASJPC010000000
VERSION     DASJPC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.218"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            262..345
                     /locus_tag="U4A13_003071"
                     /pseudo
     CDS             262..345
                     /locus_tag="U4A13_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="U4A13_003072"
     CDS             394..477
                     /locus_tag="U4A13_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEN1781284.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="U4A13_003073"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="U4A13_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN1781285.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DASJPC010000325         1380 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.226, whole genome shotgun sequence.
ACCESSION   DASJPC010000325 DASJPC010000000
VERSION     DASJPC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.226"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1311
                     /locus_tag="U4A13_003074"
     CDS             <1..1311
                     /locus_tag="U4A13_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEN1781286.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          583 a          108 c          233 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
//
LOCUS       DASJPC010000326         1354 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.229, whole genome shotgun sequence.
ACCESSION   DASJPC010000326 DASJPC010000000
VERSION     DASJPC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.229"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..302
                     /locus_tag="U4A13_003075"
                     /pseudo
     CDS             51..302
                     /locus_tag="U4A13_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            395..1189
                     /locus_tag="U4A13_003076"
                     /note="aph(3')-IIIa"
     CDS             395..1189
                     /locus_tag="U4A13_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEN1781287.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          426 a          234 c          323 g          371 t
ORIGIN      
        1 ttagacattt tcctctagga aaaaattaac tagcaccacg ggttatcttt tatatagaag
       61 atatcgccgt atgtaaggat ttcagggggc aaggcatagg cagcgcgctt atcaatatat
      121 ctatagaatg ggcaaagcat aaaaacttgc atggactaat gcttgaaacc caggacaata
      181 accttatagc ttgtaaattc tatcataatt gtggtttcaa aatcggctcc gtcgatacta
      241 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
      301 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
      361 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
      421 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
      481 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
      541 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
      601 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
      661 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
      721 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
      781 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
      841 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
      901 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
      961 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     1021 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     1081 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     1141 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctagattt
     1201 agatgtctaa aaagctttaa ctacaagctt tttagacatc taatcttttc tgaagtacat
     1261 ccgcaactgt ccatactctg atgttttata tcttttctaa aagttcgcta gataggggtc
     1321 ccgagcgcct acgaggaatt tgtatcgata agaa
//
LOCUS       DASJPC010000327         1198 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VE1354
            SAMN38756146-rid20574853.guided.239, whole genome shotgun sequence.
ACCESSION   DASJPC010000327 DASJPC010000000
VERSION     DASJPC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756146
            Sequence Read Archive: SRR27168097
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028250.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028250.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:22:29
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,077
            CDSs (total)                      :: 3,019
            Genes (coding)                    :: 2,950
            CDSs (with protein)               :: 2,950
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 27 of 69
            Pseudo Genes (incomplete)         :: 29 of 69
            Pseudo Genes (internal stop)      :: 20 of 69
            Pseudo Genes (multiple problems)  :: 6 of 69
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756146-rid20574853.guided.239"
                     /isolate="VE1354"
                     /isolation_source="Rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2019-01-10"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            259..>1198
                     /gene="vanY"
                     /locus_tag="U4A13_003077"
     CDS             259..>1198
                     /gene="vanY"
                     /locus_tag="U4A13_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEN1781288.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSNK"
BASE COUNT          428 a          173 c          240 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaataagg
//
